sp|Q0DJ99|COPD2_ORYSJ ----> Coatomer subunit delta-2

Intron 1 GTACAATAGA ... TATGTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.3	189	24.3	64
A2	21.13	168	37.3	45
A3	24.87	145	21	48
A4	25.14	137	19.9	46
A5	22.03	46	10.6	13
A6	25.9	329	43.4	115
A7	22.54	336.9	50.1	98
A8	25.43	302	42.4	103
A9	24.27	337	53.9	108
A10	22.78	278	44.3	82

>TRINITY_DN116364_c0_g2_i1 from sample A1 / Example spliced isoform

CCTCTCGCTCTCACCACTCCCAAAAATCCCGAGAACTACATTTGTTTATACAGCCATTTGCAAGGTTTTACGGTCGGGTTTCCATTTAACCTTTATTCATATAGAAAATAAAACAAGCATCCCATTATACGGAACACTCCGCAAGGATTATCGCAGGGTGCAATTACAGTCTTTGTCTCTAAATCTTCAGATCCGGGAGA
ACTATCGAGATCGAGCGATGGCGGGGGTTTTGTAATTGCGATTCGAAGTTTTCATTTGACCTTTAATTCTTTCCGGCGATCGACTGAGCACGTTCTGGAATTATCAGGATCTTCAAAATGGTGGTTCTAGCAGCATCCATTATTACGAAAACAGGGAAAGCCCTGGTGTCAAGGCAGTTTGTTGACATGTCGAGAATCAG
GATTGAAGGATTACTTGCGGCTTTCCCCAAATTGGTTGGCATTGGAAAACAACATACATATGTGGAGACAGAAAATGTTCGTTATGTGTACCAGCCAATGGAAGCTTTGTACTTGCTGCTTGTTACCAATAAGCAGAGCAACATCCTTGATGATCTTGAAACCTTAAGGGTGCTTTCCAAAATTGTGCCTGAATACTCAG
CTTCATTGGACGAAGAAGGGATTTGCAGAATGGCATTTGAGCTAATTTTTGCCTTTGATGAAGTCATCTCCCTTGGGCACAAAGAGAGCGTCACAATTGCACAGGTCAAGCAATACTGTGAGATGGAGAGTCATGAAGAACGACTCCACAAACTAATAATGCAAAGCAAGATAAATGAGACCAAGGATGTTATGAAACGA
AAAGCAAGTGAGATTGATAAAAGCAAGATTGATAGGAACAGGTCTGATAAAGGAGGCTTAATGTCATTACAATCATCTATGTCGACAAGAATTGAAAATGGTTTTGGTGATATGAGTATATCTGGTAGTGGAGGTGGCTTTGGGAGCAACTCTAGTTACGGCGGATCAATGGATTTGGATTCATTTTCAAGTAAACCAAA
AGGTCGCACATCTACAGCCGCTACAGCTCCTTCTAAGGGTCTTGGCATGCAACTAGGCAAGGCTCAAAAAACAAACCAGTTTTTGGAATCTTTGAAGGCAGAAGGAGAAGTTATTGTTGAGGATGTGCAGCCTGTTGTGGGCCAATCTAGAGGAACTGTTCCTGTTGCAACAGATCCCGTTACATTAACAGTTGAAGAAA
AGCTCAATGTGGTTCTCAAGCGTGATGGTGGATTGAATAACTTAGAAGTACAAGGTACCCTTGCCCTTCAAGTTCTGCAGAAAGACGATGGATTCATACAGGTTCAGATTGAGACCGGAGCAAATAAAGGCTTTCAATTTAAGACACATCCAAACATCAACAAGGAGTTATTTTCAAAGGAGAATTTGTTAGGACTCAAG
GATTCTAGCAGGCCTTTTCCTGCTGGTCCAACTGGTGATGCACTAGGTGTGGGTCTGGTAAAGTGGAGAATGCAAAGTGCGGATGAGACACTGGTGCCTTTGAGTATCAATTGTTGGCCATCCATTTCTGGTGGGGAAACATATGTTAATATCGAGTATGAAGCATCAAAGATGTTTGATCTACAGAATGTTATAATATC
AATACCGTTACCTGCCATCCGAGACCCACCAACTGTGAATCAGGTCGATGGAGATTGGAGATATGATTCAAGGAGGTCTGTATTAGAGTGGACCATTCTTCTTATAGATAATTCAAATTCCAGTGGATCTATGGAGTTTGTGGTGCCTAAAGCAGACCCATCAGCATTCTTTCCAATAGATGTGAGATTTACAGCTGCCA
GAACTTTCTGTGATATCAAGGTTGCTAGTGTGTTACCACTAAAAGGAGGACCACCTCCAAAATTTGCTTATAGAACACAGATGGTGACAGAGAACTACCAGGTTGTGTGAGTGTTAAAAATCCCTTTTTGTAGGGATTATTTTTGAAGATGTTGTAACAGTGGAAAGTTTTTAAAATAAACATTTAATTCTGTTATCTGT
TTTAATTTACCATCCAGTCGAACACTACAATGATACTTGTCTTTGTTCAACAAGTTTCCACCCATTTTTACTAGCTATCGCCATTCTTTTTGATTTGGTACTATTTTCCGTTCAATATTTCCGTAATTATTCTTTGATGCGAATATATGTATTTTTTTCAAACCAAGGAACAACTTCCAAAGAGCTTCTGACATTGATTT
TGCAGATTTTGAAAGAAACATGATAAATACTTCTCTCTTTAATCATTATTTTGTTTCTAGTTATACTTTTGGCCTGTGGAGTATTCTTATCATGTAAATGATTCAACCCTACTGTT

>TRINITY_DN116364_c0_g2_i2 from sample A1 / Example unspliced isoform:

CAAGTGAGATTGATAAAAGCAAGgtacaatagagaatgtattttttgaactaccggcttgccctgcaaactgacaggttactatttaagtttgtattcagattttttacctatattctatgtgacagATTGATAGGAACAGGTCTGATAAAGGAGGCTTAATGTCATTACAATCATCTATGTCGACAAGAATTGAAAATG
GTTTTGGTGATATGAGTATATCTGGTAGTGGAGGTGGCTTTGGGAGCAACTCTAGTTACGGCGGATCAATGGATTTGGATTCATTTTCAAGTAAACCAAAAGGTCGCACATCTACAGCCGCTACAGCTCCTTCTAAGGGTCTTGGCATGCAACTAGGCAAGGCTCAAAAAACAAACCAGTTTTTGGAATCTTTGAAGGCA
GAAGGAGAAGTTATTGTTGAGGATGTGCAGCCTGTTGTGGGCCAATCTAGAGGAACTGTTCCTGTTGCAACAGATCCCGTTACATTAACAGTTGAAGAAAAGCTCAATGTGGTTCTCAAGCGTGATGGTGGATTGAATAACTTAGAAGTACAAGGTACCCTTGCCCTTCAAGTTCTGCAGAAAGACGATGGATTCATACA
GGTTCAGATTGAGACCGGAGCAAATAAAGGCTTTCAATTTAAGACACATCCAAACATCAACAAGGAGTTATTTTCAAAGGAGAATTTGTTAGGACTCAAGGATTCTAGCAGGCCTTTTCCTGCTGGTCCAACTGGTGATGCACTAGGTGTGGGTCTGGTAAAGTGGAGAATGCAAAGTGCGGATGAGACACTGGTGCCTT
TGAGTATCAATTGTTGGCCATCCATTTCTGGTGGGGAAACATATGTTAATATCGAGTATGAAGCATCAAAGATGTTTGATCTACAGAATGTTATAATATCAATACCGTTACCTGCCATCCGAGACCCACCAACTGTGAATCAGGTCGATGGAGATTGGAGATATGATTCAAGGAGGTCTGTATTAGAGTGGACCATTCTT
CTTATAGATAATTCAAATTCCAGTGG

Intron 2 GTACAGCAAA ... TCCGTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	13.4	168	0	26
A2	11.58	168	0	22
A3	6.18	167	0	11
A4	9.26	147	1	15
A5	6.67	70	0	5
A6	13.64	551	0	87
A7	11.55	490	0	64
A8	12.22	352	0	49
A9	12.93	451	0	67
A10	14.75	370	0	64

>TRINITY_DN104196_c10_g1_i3 from sample A4 / Example spliced isoform

AGGTACAATAGAGAATGTATTTTTTGAACTACCGGCTTGCCCTGCAAACTGACAGGTTACTATTTAAGTTTGTATTCAGATTTTTTACCTATATTCTATGTGACAGATTGATAGGAACAGGTCTGATAAAGGAGGCTTAATGTCATTACAATCATCTATGTCGACAAGAATTGAAAATGGTTTTGGTGATATGAGTATAT
CTGGTAGTGGAGGTGGCTTTGGGAGCAACTCTAGTTACGGCGGATCAATGGATTTGGATTCATTTTCAAGTAAACCAAAAGGTCGCACATCTACAGCCGCTACAGCTCCTTCTAAGGGTCTTGGCATGCAACTAGGCAAGGCTCAAAAAACAAACCAGTTTTTGGAATCTTTGAAGGCAGAAGGAGAAGTTATTGTTGAG
GATGTGCAACCTGTTGTGGGCCAATCTAGAGGAACTGTTCCTGTTGCAACAGATCCCATTACATTAACAGTTGAAGAAAAGCTCAATGTGGTTCTCAAGCGTGATGGTGGATTGAATAACTTAGAAGTACAAGGTACCCTTGCCCTTCAAGTTCTGCAGAAAGACGATGGATTCATACAGGTTCAGATTGAGACCGGAGC
AAATAAAGGCTTTCAATTTAAGACACATCCAAACATCAACAAGGAGTTATTTTCAAAGGAGAATTTGTTAGGACTCAAGGATTCTAGCAGGCCTTTTCCTGCTGGTCCAACTGGTGATGCACTGGGTGTGGGTCTGGTAAAGTGGAGAATGCAAAGTGCGGATGAGACACTGGTGCCTTTGAGTA

>TRINITY_DN104196_c10_g1_i2 from sample A4 / Example unspliced isoform:

ACGATGGATTCATACAGGTTCAGgtacagcaaaatatttttttctgcaagttttattcttcttaaatcttgggttttgcaatacagcatgctgtttttacatgttaaaaggttggtacaaatgttggtcatttaatataaatgaactctaatgagagttccgtgtcagATTGAGACCGGAGCAAATAAAGGCTTTCAATT
TAAGACACATCCAAACATCAACAAGGAGTTATTTTCAAAGGAGAATTTGTTAGGACTCAAGGATTCTAGCAGGCCTTTTCCTGCTGGTCCAACTGGTGATGCACTGGGTGTGGGTCTGGTAAAGTGGAGAATGCAAAGTGCGGATGAGACACTGGTGCCTTTGAGTATCAATTGTTGGCCATCAATTTCTGGTGGGGAAA
CATATGTTAATATCGAGTATGAAGCATCAAAGATGTTTGATCTACAGAATGTTATAATATCAATACCGTTACCTGCCATCCGAGACCCACCAACTGTGAATCAGGTCGATGGAGATTGGAGATATGATTCAAGGAGGTCTGTATTAGAGTGGACCATTCTTCTTATAGATAATTCAAATTCCAGTGGATCTATGGAGTTT
GTGGTGCCTAAAGCAGACCCATCAGCATTCTTTCCAATAGATGTGAGATTTACAGCTGCCAGAACTTTCTGTGATATCAAGGTTGCTAGTGTGTTACCACTAAAAGGAGGACCACCTCCAAAATTTGCTTATAGAACACAGATGGTGACAGAGAACTACCAGGTTGTGTGAGTGTTAAAAATCCCTTTTTGTAGGGATTA
TTTTTGAAGATGTTGTAACAGTGGAAAGTTTTTAAAATAAACATTTAATTCTGTTATCTGTTTTAATTTACCATCCAGTCGAACACTACAATGATACTTGTCTTTGTTCAACAAGTTTCCACCCATTTTTACTTGCTATCGCCATTCTTTTTGATTTGGTACTATTTTCCGTTCAATATTTCCGTAATTATTCTTTGATG
CGAATATATGTATTTTTTTCAAACCAAGGAACAACTTCCAAAGAGCTTCTGACATTGATTTTGCAGATTTTGAAAGAAACATGATAAATACTTC

Intron 3 GTTAGTTGTT ... GATCATTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.71	222	58	0
A2	25.5	225	77	1
A3	18.31	241	54	0
A4	25.18	208	70	2
A5	26.67	110	40	1
A6	17.2	597	124	1
A7	20.1	648	163	2
A8	14.62	444	76	0
A9	17.13	508	105	0
A10	21.28	429	116	1

>TRINITY_DN42374_c0_g1_i1 from sample A10 / Example spliced isoform

GTTTTACGGCTTAAAAAAACCCTCTCGCTCTCACCACTCCCAAAAATCCCGAGAACTACATTTGTTTCTACAGCCATTTGTAAGGTTTTACGGTCGGGTTTCCATTTAACCTTTATTCATATAGAAAATAAAACAAGCATCCCATTATACGGAACACTCCGCAAGGATTATCACAGGGTGCAATTACAGTCTTTGTCTCT
AAATCTTCAGATCCGGGAGAACTATCGAGATCGAGCGATGGCGGGGGTTTTGTAATTGCGATTCGAAGTTTTCATTTGACCTTTAATTCTTTCCGGCGATCGACTGAGCACGTTCTGGAATTATCAGGATCTTCAAAATGTTGGAATTGAGGTAGATGCTTTCTACAAGTTTTGAGATTTTGGCTGCAGGTGGTTCTAGC
AGCATCCATTATTACGAAAACAGGGAAAGCCCTGGTGTCAAGGCAGTTTGTTGACATGTCGAGAATCAGGATTGAAGGATTACTTGCGGCTTTCCCCAAATTGGTTGGCATTGGAAAACAACATACATATGTGGAGACAGAAAATGTTCGTTATGTGTACCAGCCAATGGAAGCTTTGTACTTGCTGCTTGTTACCAATA
AGCAGAGCAACATCCTTGATGATCTTGAAACCTTAAGGGTGCTTTCCAAAATTGTGCCTGAATACTCAGCTTCATTGGACGAAGAAGGGATTTGCAGAATGGCATTTGAGCTAATTTTTGCCTTTGATGAAGTCATCTCCCTTGGGCACAAAGAGAGCGTCACAATTGCACAGGTCAAGCAATACTGTGAGATGGAGAGT
CATGAAGAACGACTCCACAAACTAATAATGCAAAGCAAGATAAATGAGACCAAGGATGTTATGAAACGAAAAGCAAGTGAGATTGATAAAAGCAAGATTGATAGGAACAGGTCTGATAAAGGAGGCTTAATGTCATTACAATCATCTATGTCGACAAGAATTGAAAATGGTTTTGGTGATATGAGTATATCTGGTAGTGG
AGGTGGCTTTGGGAGCAACTCTAGTTACGGCGGATCAATGGATTTGGATTCATTTTCAAGTAAACCAAAAGGTCGCACATCTACAGCCGCTACAGCTCCTTCTAAGGGTCTTGGCATGCAACTAGGCAAGGCTCAAAAAACAAACCAGTTTTTGGAATCTTTGAAGGCAGAAGGAGAAGTTATTGTTGAGGATGTGCAGC
CTGTTGTGGGCCAATCTAGAGGAACTGTTCCTGTTGCAACAGATCCCATTACATTAACAGTTGAAGAAAAGCTCAATGTGGTTCTCAAGCGTGATGGTGGATTGAATAACTTAGAAGTACAAGGTACCCTTGCCCTTCAAGTTCTGCAGAAAGACGATGGATTCATACAGGTTCAGATTGAGACCGGAGCAAATAAAGGC
TTTCAATTTAAGACACATCCAAACATCAACAAGGAGTTATTTTCAAAGGAGAATTTGTTAGGACTCAAGGATTCTAGCAGGCCTTTTCCTGCTGGTCCAACTGGTGATGCACTAGGTGTGGGTCTGGTAAAGTGGAGAATGCAAAGTGCGGATGAGACACTGGTGCCTTTGAGTATCAATTGTTGGCCATCCATTTCTGG
TGGGGAAACATATGTTAATATCGAGTATGAAGCATCAAAGATGTTTGATCTACAGAATGTTATAATATCAATACCGTTACCTGCCATCCGAGACCCACCAACTGTGAATCAGGTCGATGGAGATTGGAGATATGATTCAAGGAGGTCTGTATTAGAGTGGACCATTCTTCTTATAGATAATTCAAATTCCAGTGGATCTA
TGGAGTTTGTGGTGCCTAAAGCAGACCCATCAGCATTCTTTCCAATAGATGTGAGATTTACAGCTGCCAGAACTTTCTGTGATATCAAGGTTGCTAGTGTGTTACCACTAAAAGGAGGACCACCTCCAAAATTTGCTTATAGAACACAGATGGTGACAGAGAACTACCAGGTTGTGTGAGTGTTAAAAATCCCTTTTTGT
AGGGATTATTTTTGAAGATGTTGTAACAGTGGAAAGTTTTTAAAATAAACATTTAATTCTGTTATCTGTTTTAATTTACCATCCAGTCGAACACTACAATGATACTTGTCTTTGTTCAACAAGTTTCCACCCATTTTTACTTGCTATCGCCATTCTTTTTGATTTGGTACTATTTTCCGTTCAATATTTCCGTAATTATT
CTTTGATGCGAATATATGTATTTTTTTCAAACCAAGGAACAACTTCCAAAGAGCTTCTGACATTGATTTTGCAGATTTTGAAAGAAACATGATAAATAATTCTCTCTTTAATCATTATTTTGTTTCTAGTTATACTTTTGGCCTGTGGAGTATTCTTATCATGTAAATGATTCAACCCTACTGTTAGACAT

>TRINITY_DN42374_c0_g1_i2 from sample A10 / Example unspliced isoform:

GTTTTACGGCTTAAAAAAACCCTCTCGCTCTCACCACTCCCAAAAATCCCGAGAACTACATTTGTTTCTACAGCCATTTGTAAGGTTTTACGGTCGGGTTTCCATTTAACCTTTATTCATATAGAAAATAAAACAAGCATCCCATTATACGGAACACTCCGCAAGGATTATCACAGGGTGCAATTACAGTCTTTGTCTCT
AAATCTTCAGATCCGGGAGAACTATCGAGATCGAGCGATGGCGGGGGTTTTGTAATTGCGATTCGAAGTTTTCATTTGACCTTTAATTCTTTCCGGCGATCGACTGAGCACGTTCTGGAATTATCAGGATCTTCAAAATGGTGGTTCTAGCAGCATCCATTATTACGAAAACAGGGAAAGCCCTGGTGTCAAGGCAGTTT
GTTGACATGTCGAGAATCAGGATTGAAGGATTACTTGCGGCTTTCCCCAAATTGGTTGGCATTGGAAAACAACATACATATGTGGAGACAGAAAATGTTCGTTATGTGTACCAGCCAATGGAAGCTTTGTACTTGCTGCTTGTTACCAATAAGCAGAGCAACATCCTTGATGATCTTGAAACCTTAAGGGTGCTTTCCAA
AATTGTGCCTGAATACTCAGCTTCATTGGACGAAGAAGGGATTTGCAGAATGGCATTTGAGCTAATTTTTGCCTTTGATGAAGTCATCTCCCTTGGGCACAAAGAGAGCGTCACAATTGCACAGGTCAAGCAATACTGTGAGATGGAGAGTCATGAAGAACGACTCCACAAACTAATAATGCAAAGCAAGATAAATGAGA
CCAAGGATGTTATGAAACGAAAAGCAAGTGAGATTGATAAAAGCAAGATTGATAGGAACAGGTCTGATAAAGGAGGCTTAATGTCATTACAATCATCTATGTCGACAAGAATTGAAAATGGTTTTGGTGATATGAGTATATCTGGTAGTGGAGGTGGCTTTGGGAGCAACTCTAGTTACGGCGGATCAATGGATTTGGAT
TCATTTTCAAGTAAACCAAAAGgttagttgttattttgcaaatttcaattcaagcaggcctcaagtgaactataatgtatactaatattttatatctattttgaatcattattgcctgatatttttatacttaaattattcgatcatttagGTCGCACATCTACAGCCGC

Intron 4 GTGAGTCTAA ... TGCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.82	210	11	29
A2	11.17	197	17	28
A3	7.26	206	18.5	17.5
A4	14.98	144	14.5	29
A5	10.94	75	7	10.5
A6	9.46	978.5	75	114
A7	9.79	722.5	48.5	88
A8	9.92	742	56	90.5
A9	9.54	649	41	76.5
A10	8.65	545	39	57

>TRINITY_DN104196_c10_g1_i1 from sample A4 / Example spliced isoform

CTCGCTCTCACCACTCCCAAAAATCCCGAGAACTACATTTGTTTCTACAGCCATTTGTAAGGTTTTACGGTCGGGTTTCCATTTAACCTTTATTCATATAGAAAATAAAACAAGCATCCCATTATACGGAACACTCCGCAAGGATTATCACAGGGTGCAATTACAGTCTTTGTCTCTAAATCTTCAGATCCGGGAGAACT
ATCGAGATCGAGCGATGGCGGGGGTTTTGTAATTGCGATTCGAAGTTTTCATTTGACCTTTAATTCTTTCCGGCGATCGACTGAGCACGTTCTGGAATTATCAGGATCTTCAAAATGGTGGTTCTAGCAGCATCCATTATTACGAAAACAGGGAAAGCCCTGGTGTCAAGGCAGTTTGTTGACATGTCGAGAATCAGGAT
TGAAGGATTACTTGCGGCTTTCCCCAAATTGGTTGGCATTGGAAAACAACATACATATGTGGAGACAGAAAATGTTCGTTATGTGTACCAGCCAATGGAAGCTTTGTACTTGCTGCTTGTTACCAATAAGCAGAGCAACATCCTTGATGATCTTGAAACCTTAAGGGTGCTTTCCAAAATTGTGCCTGAATACTCAGCTT
CATTGGACGAAGAAGGGATTTGCAGAATGGCATTTGAGCTAATTTTTGCCTTTGATGAAGTCATCTCCCTTGGGCACAAAGAGAGCGTCACAATTGCACAGGTCAAGCAATACTGTGAGATGGAGAGTCATGAAGAACGACTCCACAAACTAATAATGCAAAGCAAGATAAATGAGACCAAGGATGTTATGAAACGAAAA
GCAAGTGAGATTGATAAAAGCAAGATTGATAGGAACAGGTCTGATAAAGGAGGCTTAATGTCATTACAATCATCTATGTCGACAAGAATTGAAAATGGTTTTGGTGATATGAGTATATCTGGTAGTGGAGGTGGCTTTGGGAGCAACTCTAGTTACGGCGGATCAATGGATTTGGATTCATTTTCAAGTAAACCAAAAGG
TCGCACATCTACAGCCGCTACAGCTCCTTCTAAGGGTCTTGGCATGCAACTAGGCAAGGCTCAAAAAACAAACCAGTTTTTGGAATCTTTGAAGGCAGAAGGAGAAGTTATTGTTGAGGATGTGCAACCTGTTGTGGGCCAATCTAGAGGAACTGTTCCTGTTGCAACAGATCCCATTACATTAACAGTTGAAGAAAAGC
TCAATGTGGTTCTCAAGCGTGATGGTGGATTGAATAACTTAGAAGTACAAGGTACCCTTGCCCTTCAAGTTCTGCAGAAAGACGATGGATTCATACAGGTTCAGATTGAGACCGGAGCAAATAAAGGCTTTCAATTTAAGACACATCCAAACATCAACAAGGAGTTATTTTCAAAGGAGAATTTGTTAGGACTCAAGGAT
TCTAGCAGGCCTTTTCCTGCTGGTCCAACTGGTGATGCACTGGGTGTGGGTCTGGTAAAGTGGAGAATGCAAAGTGCGGATGAGACACTGGTGCCTTTGAGTATCAATTGTTGGCCATCAATTTCTGGTGGGGAAACATATGTTAATATCGAGTATGAAGCATCAAAGATGTTTGATCTACAGAATGTTATAATATCAAT
ACCGTTACCTGCCATCCGAGACCCACCAACTGTGAATCAGGTCGATGGAGATTGGAGATATGATTCAAGGAGGTCTGTATTAGAGTGGACCATTCTTCTTATAGATAATTCAAATTCCAGTGGATCTATGGAGTTTGTGGTGCCTAAAGCAGACCCATCAGCATTCTTTCCAATAGATGTGAGATTTACAGCTGCCAGAA
CTTTCTGTGATATCAAGGTTGCTAGTGTGTTACCACTAAAAGGAGGACCACCTCCAAAATTTGCTTATAGAACACAGATGGTGACAGAGAACTACCAGGTTGTGTGAGTGTTAAAAATCCCTTTTTGTAGGGATTATTTTTGAAGATGTTGTAACAGTGGAAAGTTTTTAAAATAAACATTTAATTCTGTTATCTGTTTT
AATTTACCATCCAGTCGAACACTACAATGATACTTGTCTTTGTTCAACAAGTTTCCACCCATTTTTACTTGCTATCGCCATTCTTTTTGATTTGGTACTATTTTCCGTTCAATATTTCCGTAATTATTCTTTGATGCGAATATATGTATTTTTTTCAAACCAAGGAACAACTTCCAAAGAGCTTCTGACATTGATTTTGC
AGATTTTGAAAGAAACATGATAAATACTTC

>TRINITY_DN104196_c10_g1_i5 from sample A4 / Example unspliced isoform:

CTCGCTCTCACCACTCCCAAAAATCCCGAGAACTACATTTGTTTCTACAGCCATTTGTAAGGTTTTACGGTCGGGTTTCCATTTAACCTTTATTCATATAGAAAATAAAACAAGCATCCCATTATACGGAACACTCCGCAAGGATTATCACAGGGTGCAATTACAGTCTTTGTCTCTAAATCTTCAGATCCGGGAGAACT
ATCGAGATCGAGCGATGGCGGGGGTTTTGTAATTGCGATTCGAAGTTTTCATTTGACCTTTAATTCTTTCCGGCGATCGACTGAGCACGTTCTGGAATTATCAGGATCTTCAAAATGGTGGTTCTAGCAGCATCCATTATTACGAAAACAGGGAAAGCCCTGGTGTCAAGGCAGTTTGTTGACATGTCGAGAATCAGGAT
TGAAGGATTACTTGCGGCTTTCCCCAAATTGGTTGGCATTGGAAAACAACATACATATGTGGAGACAGAAAATGTTCGTTATGTGTACCAGCCAATGGAAGCTTTGTACTTGCTGCTTGTTACCAATAAGCAGAGCAACATCCTTGATGATCTTGAAACCTTAAGGGTGCTTTCCAAAATTGTGCCTGAATACTCAGCTT
CATTGGACGAAGAAGGGATTTGCAGAATGGCATTTGAGCTAATTTTTGCCTTTGATGAAGTCATCTCCCTTGGGCACAAAGAGAGCGTCACAATTGCACAGGTCAAGCAATACTGTGAGATGGAGAGTCATGAAGAACGACTCCACAAACTAATAATGCAAAGCAAGATAAATGAGACCAAGGATGTTATGAAACGAAAA
GCAAGTGAGATTGATAAAAGCAAGATTGATAGGAACAGGTCTGATAAAGGAGGCTTAATGTCATTACAATCATCTATGTCGACAAGAATTGAAAATGGTTTTGGTGATATGAGTATATCTGGTAGTGGAGGTGGCTTTGGGAGCAACTCTAGTTACGGCGGATCAATGGATTTGGATTCATTTTCAAGTAAACCAAAAGG
TCGCACATCTACAGCCGCTACAGCTCCTTCTAAGGGTCTTGGCATGCAACTAGGCAAGGCTCAAAAAACAAACCAGTTTTTGGAATCTTTGAAGGCAGAAGGAGAAGTTATTGTTGAGGATGTGCAACCTGTTGTGGGCCAATCTAGAGGAACTGTTCCTGTTGCAACAGATCCCATTACATTAACAGTTGAAGAAAAGC
TCAATGTGGTTCTCAAGCGTGATGGTGGATTGAATAACTTAGAAGTACAAGGTACCCTTGCCCTTCAAGTTCTGCAGAAAGACGATGGATTCATACAGGTTCAGATTGAGACCGGAGCAAATAAAGGCTTTCAATTTAAGACACATCCAAACATCAACAAGGAGTTATTTTCAAAGGAGAATTTGTTAGGACTCAAGGAT
TCTAGCAGGCCTTTTCCTGCTGGTCCAACTGGTGATGCACTGGGTGTGGGTCTGGTAAAGTGGAGAATGCAAAGTGCGGATGAGACACTGGTGCCTTTGAGTAgtgagtctaatcgtttttaaattttttaatatcttaaaactttttattacttttttttctttggttacgatgatgttgaaagtggacaatatttttg
ttggagaactatttcgtctaaaagactaaaatatgtgtgcgaattatgaaagcagtctttgctctttttgttctcacagatttagattttcattttccaggtacaccatttgttaggaaagtgatatacacaaaatattgggatataaacgtcgtaatgactatttgttttatgtgctttgcagTCAATTGTTGGCCATC
CATTTCTGGTGGGGAAACATATGT

Intron 5 GTTAGCTTAG ... CCATATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.81	138	0	48
A2	23.79	173	1	54
A3	20.88	144	1	38
A4	22.73	102	0	30
A5	33.33	64	0	32
A6	26.05	1042	1	367
A7	25.54	726	0	249
A8	26.58	721	1	261
A9	25.87	705	1	246
A10	23.93	550	0	173

>TRINITY_DN48807_c0_g6_i2 from sample A7 / Example spliced isoform

AAACCCTTTCGCTCTCACCTCTCCCAAAAATCCCGAGAACTACATTTGTTTCTACAGCCATTTGTAAGGTTTTACGGTCGGGTTTCCATTTAACCTTTATTCATATAGAAAATAAAACAAGCATCCCATTATACGGAACACTCCGCAAGGATTATCACAGGGTGCAATTACAGTCTTTGTCTCTAAATCTTCAGATCCGG
GAGAACTATCGAGATCGAGCGATGGCGGGGGTTTTGTAATTGCGATTCGAAGTTTTCATTTGACCTTTAATTCTTTCCGGCGATCGACTGAGCACGTTCTGGAATTATCAGGATCTTCAAAATGGTGGTTCTAGCAGCATCCATTATTACGAAAACAGGGAAAGCCCTGGTGTCAAGGCAGTTTGTTGACATGTCGAGAA
TCAGGATTGAAGGATTACTTGCGGCTTTCCCCAAATTGGTTGGCATTGGAAAACAACATACATATGTGGAGACAGAAAATGTTCGTTATGTGTACCAGCCAATGGAAGCTTTGTACTTGCTGCTTGTTACCAATAAGCAGAGCAACATCCTTGATGATCTTGAAACCTTAAGGGTGCTTTCCAAAATTGTGCCTGAATAC
TCAGCTTCATTGGACGAAGAAGGGATTTGCAGAATGGCATTTGAGCTAATTTTTGCCTTTGATGAAGTCATCTCCCTTGGGCACAAAGAGAGCGTCACAATTGCACAGGTCAAGCAATACTGTGAGATGGAGAGTCATGAAGAACGACTCCACAAACTAATAATGCAAAGCAAGATAAATGAGACCAAGGATGTTATGAA
ACGAAAAGCAAGTGAGATTGATAAAAGCAAGATTGATAGGAACAGGTCTGATAAAGGAGGCTTAATGTCATTACAATCATCTATGTCGACAAGAATTGAAAATGGTTTTGGTGATATGAGTATATCTGGTAGTGGAGGTGGCTTTGGGAGCAACTCTAGTTACGGCGGATCAATGGATTTGGATTCATTTTCAAGTAAAC
CAAAAGGTCGCACATCTACAGCCGCTACAGCTCCTTCTAAGGGTCTTGGCATGCAACTAGGCAAGGCTCAAAAAACAAACCAGTTTTTGGAATCTTTGAAGGCAGAAGGAGAAGTTATTGTTGAGGATGTGCAACCTGTTGTGGGCCAATCTAGAGGAACTGTTCCTGTTGCAACAGATCCCGTTACATTAACAGTTGAA
GAAAAGCTCAATGTGGTTCTCAAGCGTGATGGTGGATTGAATAACTTAGAAGTACAAGGTACCCTTGCCCTTCAAGTTCTGCAGAAAGACGATGGATTCATACAGGTTCAGATTGAGACCGGAGCAAATAAAGGCTTTCAATTTAAGACACATCCAAACATCAACAAGGAGTTATTTTCAAAGGAGAATTTGTTAGGACT
CAAGGATTCTAGCAGGCCTTTTCCTGCTGGTCCAACTGGTGATGCACTGGGTGTGGGTCTGGTAAAGTGGAGAATGCAAAGTGCGGATGAGACACTGGTGCCTTTGAGTATCAATTGTTGGCCATCAATTTCTGGTGGGGAAACATATGTTAATATCGAGTATGAAGCATCAAAGATGTTTGATCTACAGAATGTTATAA
TATCAATACCGTTACCTGCCATCCGAGACCCACCAACTGTGAATCAGGTCGATGGAGATTGGAGATATGATTCAAGGAGGTCTGTATTAGAGTGGACCATTCTTCTTATAGATAATTCAAATTCCAGTGGATCTATGGAGTTTGTGGTGCCTAAAGCAGACCCATCAGCATTCTTTCCAATAGATGTGAGATTTACAGCT
GCCAGAACTTTCTGTGATATCAAGGTTGCTAGTGTGTTACCACTAAAAGGAGGACCACCTCCAAAATTTGCTTATAGAACACAGATGGTGACAGAGAACTACCAGGTTGTGTGAGTGTTAAAAATCCCTTTTTGTAGGGATTATTTTTGAAGATGTTGTAACAGTGGAAAGTTTTTAAAATAAACATTTAATTCTGTTAT
CTGTTTTAATTTACCATCCAGTCGAACACTACAATGATACTTGTCTTTGTTCAACAAGTTTCCACCCATTTTTACTAGCTATCGCCATTCTTTTTGATTTGGTACTATTTTCCGTTCAATATTTCCGTAATTATTCTTTGATGCGAATATATGTATTTTTTTCAAACCAAGGAACAACTTCCAAAGAGCTTCTGACATTG
ATTTTGCAGATTTTGAAAGAAACATGATAAATACTTCTCTCTTTAATCATTATTTTGTTTCTAGTTATACTTTTGGCCTGTGGAGTATTCTTATCATGTAAATGATTCAACCCTACTGTTAGACATACTTTTTTTGTACTTTTGATATTTAATATAGTTTTACATGGATATGGTTTTGCTCAGGAAGGCATTCCTCATGT
TTACTCACTTAGCCTTATATGTCATTACAATACCCAGGAAACGGA

>TRINITY_DN48807_c0_g6_i1 from sample A7 / Example unspliced isoform:

AATCAGGTCGATGGAGATTGGAGgttagcttagatagtagacactcttttttgttccttcaccaccttttttttgtggttttagttattaggttccttggcagccattcttttcaagtctgtaagaaattgtataagcaggatgttcacacagtagttcagcacagttttctcatgaatgattgtgattcgtgtcccata
tacagATATGATTCAAGGAGGTCTGTATTAGAGTGGACCATTCTTCTTATAGATAATTCAAATTCCAGTGGATCTATGGAGTTTGTGGTGCCTAAAGCAGACCCATCAGCATTCTTTCCAATAGATGTGAGATTTACAGCTGCCAGAACTTTCTGTGATATCAAGGTAAAGCCATATTTTACGATTTTTTCTGTAAAGCG
CAGTCATGCTGATGTACTGACATCTTCTAATGTTGGTTACACATATTACTTATGGTGTTACATGGATGCAGGTTGC