NA ----> NA

Intron 1 GTACTCTTCC ... TGATTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.92	37	1	11
A2	0	14	5	0
A3	25.49	38	1	13
A4	37.74	33	2	20
A5	34.21	25	2	13
A6	33.26	305	14	152
A7	29.97	278	12	119
A8	27.64	267	25	102
A9	30.09	244	24	105
A10	30.54	332	29	146

>TRINITY_DN40018_c0_g1_i4 from sample A8 / Example spliced isoform

ATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAA
GAAGTACAATGCATTTCCAAAAATGTGTTGGCATAGGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCAT
GGGTTTCAAGTAGCTGTTCACCCACAGGTTAAGGCTTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCA
GTGATCGATTTTTCACTTCTTCCTGCAATCACACACTGCGAACGGTGAGAGGGTAACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAGGTGTGTAGTTTGTAGATTCCGAAGGGCTAAGGAGGATTGTACTCGTTCGCAAGAACTCTCAAACCGCTATCACTTTCGATTGTTTTACCAACCATGTCTG
GGGTGTGTTTGACATAAGCAATAAGGTACGTCTGAAATATCTTAGAGACGGAGAAATAATAGTGGTAGATGATGAGGTGGGGTGGGAAGAGACCTCCATGAGTTTAAAGAACCAAGAAACCCTCAACGTCGTTCTTGAACGTTTGAATTTTGATGTGAAACTTTTGGAATGTGATGATTACAAGGGAGAGTGGGAACAGA
TCGGCTTGAATTCATTGAATTCATTTACAACTGTATGAGGAGAGATCCCTAGTTTTCTCTCACAGTAGAAATCAGACCAATTTCTGTTTATCTACAGAAAACCAGAAAATGAATTACTTCTACTAGGGCTCGGCCTGGGCAGCCATGGCTGGCCTAATTTTTTTGTTTTAGTGATAATGTGCCTCAAATATTAGGCTTCT
GTGTGGCTATCCTATCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTGATGATATGGCATATTCATCCATTGTATTTTGCTCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTG

>TRINITY_DN40018_c0_g1_i3 from sample A8 / Example unspliced isoform:

ATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAA
GAAGTACAATGCATTTCCAAAAATGTGTTGGCATAGGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCAT
GGGTTTCAAGTAGCTGTTCACCCACAGGTTAAGGCTTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCA
GTGATCGGTGAGACAAAAAGAAAGAAGATATCGAATTAACTTTCCATTTTTCACTTCTTCCTGCAATCACACACTGCGAACGGTGAGAGGGTAACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAGGTGTGTAGgtactcttcccggcctttgtgtaggaatcagaaattcagttttctatgttttct
gtgtttctttttttcttttcggcagggataggatcggtgtttctgttttactgagaaaaaataggatttaatgttctttctttgttggctgtgattatcagTTTGTAGATTCCGAAGGGCTAAGGAGGATTGTACTCGTTCGCAAGAACTCTCAAACCGCTATCACTTTCGATTGTTTTACCAACCATGTCTGGGGTGTG
TTTGACATAAGCAATAAGGTACGTCTGAAATATCTTAGAGACGGAGAAATAATAGTGGTAGATGATGAGGTGGGGTGGGAAGAGACCTCCATGAGTTTAAAGAACCAAGAAACCCTCAACGTCGTTCTTGAACGTTTGAATTTTGATGTGAAACTTTTGGAATGTGATGATTACAAGGGAGAGTGGGAACAGATCGGCTT
GAATTCATTGAATTCATTTACAACTGTATGAGGAGAGATCCCTAGTTTTCTCTCACAGTAGAAATCAGACCAATTTCTGTTTATCTACAGAAAACCAGAAAATGAATTACTTCTACTAGGGCTCGGCCTGGGCAGCCATGGCTGGCCTAATTTTTTTGTTTTAGTGATAATGTGCCTCAAATATTAGGCTTCTGTGTGGC
TATCCTATCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTGATGATATGGCATATTCATCCATTGTATTTTGCTCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTG

Intron 2 GTGTGTAGGT ... TGATTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	1.3	37.5	0.5	5.5
A2	10.64	15	2	0
A3	1.28	38	0.5	6.5
A4	6.74	34.5	2.5	10.5
A5	9.06	25	2.5	7
A6	5.04	311	16.5	76
A7	5.29	286	16	60
A8	6.29	286	19.5	51
A9	4.7	262	13.5	53.5
A10	6.38	349	24	75.5

>TRINITY_DN40018_c0_g1_i1 from sample A8 / Example spliced isoform

ATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAA
GAAGTACAATGCATTTCCAAAAATGTGTTGGCATAGGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCAT
GGGTTTCAAGTAGCTGTTCACCCACAGGTTAAGGCTTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCA
GTGATCGGTGAGACAAAAAGAAAGAAGATATCGAATTAACTTTCCGTGCGTATATCATTATATTAACTTTCGGCTGATAGATTCGTTCAGTTGCTTTCTACCTTCAGATGTTGTTCTATGGCCCGTTATTTTGTAGAGTGGTTTACCCATTTTTAAAACTTGTTCGTCTGAGCTTTCTTGCCCCTAGATTTTTCACTTCT
TCCTGCAATCACACACTGCGAACGGTGAGAGGGTAACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAGTTTGTAGATTCCGAAGGGCTAAGGAGGATTGTACTCGTTCGCAAGAACTCTCAAACCGCTATCACTTTCGATTGTTTTACCAACCATGTCTGGGGTGTGTTTGACATAAGCAATAAGGTA
CGTCTGAAATATCTTAGAGACGGAGAAATAATAGTGGTAGATGATGAGGTGGGGTGGGAAGAGACCTCCATGAGTTTAAAGAACCAAGAAACCCTCAACGTCGTTCTTGAACGTTTGAATTTTGATGTGAAACTTTTGGAATGTGATGATTACAAGGGAGAGTGGGAACAGATCGGCTTGAATTCATTGAATTCATTTAC
AACTGTATGAGGAGAGATCCCTAGTTTTCTCTCACAGTAGAAATCAGACCAATTTCTGTTTATCTACAGAAAACCAGAAAATGAATTACTTCTACTAGGGCTCGGCCTGGGCAGCCATGGCTGGCCTAATTTTTTTGTTTTAGTGATAATGTGCCTCAAATATTAGGCTTCTGTGTGGCTATCCTATCCAAACTCTGTGA
CTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTGATGATATGGCATATTCATCCATTGTATTTTGCTCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTG

>TRINITY_DN40018_c0_g1_i3 from sample A8 / Example unspliced isoform:

ATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAA
GAAGTACAATGCATTTCCAAAAATGTGTTGGCATAGGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCAT
GGGTTTCAAGTAGCTGTTCACCCACAGGTTAAGGCTTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCA
GTGATCGGTGAGACAAAAAGAAAGAAGATATCGAATTAACTTTCCATTTTTCACTTCTTCCTGCAATCACACACTGCGAACGGTGAGAGGGTAACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAGgtgtgtaggtactcttcccggcctttgtgtaggaatcagaaattcagttttctatgttttct
gtgtttctttttttcttttcggcagggataggatcggtgtttctgttttactgagaaaaaataggatttaatgttctttctttgttggctgtgattatcagTTTGTAGATTCCGAAGGGCTAAGGAGGATTGTACTCGTTCGCAAGAACTCTCAAACCGCTATCACTTTCGATTGTTTTACCAACCATGTCTGGGGTGTG
TTTGACATAAGCAATAAGGTACGTCTGAAATATCTTAGAGACGGAGAAATAATAGTGGTAGATGATGAGGTGGGGTGGGAAGAGACCTCCATGAGTTTAAAGAACCAAGAAACCCTCAACGTCGTTCTTGAACGTTTGAATTTTGATGTGAAACTTTTGGAATGTGATGATTACAAGGGAGAGTGGGAACAGATCGGCTT
GAATTCATTGAATTCATTTACAACTGTATGAGGAGAGATCCCTAGTTTTCTCTCACAGTAGAAATCAGACCAATTTCTGTTTATCTACAGAAAACCAGAAAATGAATTACTTCTACTAGGGCTCGGCCTGGGCAGCCATGGCTGGCCTAATTTTTTTGTTTTAGTGATAATGTGCCTCAAATATTAGGCTTCTGTGTGGC
TATCCTATCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTGATGATATGGCATATTCATCCATTGTATTTTGCTCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTG

Intron 3 GTGCGTATAT ... TTGCCCCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.64	16.9	9	7.4
A2	17.33	20	6	2.4
A3	28.77	12	4	5.8
A4	45.45	6	5	5
A5	66.68	4	4	12.1
A6	32.3	99.9	36	59.9
A7	34.92	119.8	75	54
A8	33.97	110.1	50	63.9
A9	27.87	108.8	47	37.4
A10	32.69	144.7	72	69.1

>TRINITY_DN113574_c0_g1_i1 from sample A1 / Example spliced isoform

ATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGGCATA
GGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCATGGGTTTCAAGTAGCTGTTCACCCACAGGTTAAGGC
TTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCAGTGATCGGTGAGACAAAAAGAAAGAAGATATCGAA
TTAACTTTCCATTTTTCACTTCTTCCTGCAATCACACACTGCGAACGGTGAGAGGGTAACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAGGTGTGTAGTTTGTAGATTCCGAAGGGCTAAGGAGGATTGTACTCGTTCGCAAGAACTCTCAAACCGCTATCACTTTCGATTGTTTTACCAACCATGT
CTGGGGTGTGTTTGACATAAGCAATAAGGTACGTCTGAAATATCTTAGAGACGGAGAAATAATAGTGGTAGATGATGAGGTGGGGTGGGAAGAGACCTCCATGAGTTTAAAGAACCAAGAAACCCTCAACGTCGTTCTTGAACGTTTGAATTTTGATGTGAAACTTTTGGAATGTGATGATTACAAGGGAGAGTGGGAAC
AGATCGGCTTGAATTCATTGAATTCATTTACAACTGTATGAGGAGAGATCCCTAGTTTTCTCTCACAGTAGAAATCAGACCAATTTCTGTTTATCTACAGAAAACCAGAAAATGAATTACTTCTACTAGGGCTCGGCCTGGGCAGCCATGGCTGGCCTAATTTTTTTGTTTTAGTGATAATGTGCCTCAAATATTAGGCT
TCTGTGTGGCTATCCTATCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTGATGATATGGCATATTCATCCATTGTATTTTGCTCCAAACTCTGTGACTTCACAGCCCTTTTGTTATG

>TRINITY_DN113574_c0_g1_i6 from sample A1 / Example unspliced isoform:

ATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGGCATA
GGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCATGGGTTTCAAGTAGCTGTTCACCCACAGGTTAAGGC
TTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCAGTGATCGGTGAGACAAAAAGAAAGAAGATATCGAA
TTAACTTTCCgtgcgtatatcattatattaactttcggctgatagattcgttcagttgctttctaccttcagatgttgttctatggcccgttattttgtagagtggtttacccatttttaaaacttgttcgtctgagctttcttgcccctagATTTTTCACTTCTTCCTGCAATCACACACTGCGAACGGTGAGAGGGTA
ACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAG

Intron 4 GTGTGTAGGT ... TGATTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.3	37.7	0	5.5
A2	0	13.7	0	0
A3	12.74	38	0	6.5
A4	19.82	34.3	3	10.5
A5	19.03	25	2	7
A6	16.54	308.5	16	76
A7	14.9	285	14	60
A8	13.4	281	10	51
A9	14.8	260.5	1	53.5
A10	15.67	344.3	16	75.5

>TRINITY_DN44172_c0_g1_i5 from sample A7 / Example spliced isoform

ACCATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGGC
ATAGGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCATGGGTTTCAAGTAGCTGTTCACCCACAGGTTAA
GGCTTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCAGTGATCGGTGAGACAAAAAGAAAGAAGATATC
GAATTAACTTTCCGTGCGTATATCATTATATTAACTTTCGGCTGATAGATTCGTTCAGTTGCTTTCTACCTTCAGATGTTGTTCTATGGCCCGTTATTTTGTAGAGTGGTTTACCCATTTTTAAAACTTGTTCGTCTGAGCTTTCTTGCCCCTAGATTTTTCACTTCTTCCTGCAATCACACACTGCGAACGGTGAGAGG
GTAACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAGTTTGTAGATTCCGAAGGGCTAAGGAGGATTGTACTCGTTCGCAAGAACTCTCAAACCGCTATCACTTTCGATTGTTTTACCAACCATGTCTGGGGTGTGTTTGACATAAGCAATAAGGTACGTCTGAAATATCTTAGAGACGGAGAAATAAT
AGTGGTAGATGATGAGGTGGGGTGGGAAGAGACCTCCATGAGTTTAAAGAACCAAGAAACCCTCAACGTCGTTCTTGAACGTTTGAATTTTGATGTGAAACTTTTGGAATGTGATGATTACAAGGGAGAGTGGGAACAGATCGGCTTGAATTCATTGAATTCATTTACAACTGTATGAGGAGAGATCCCTAGTTTTCTCT
CACAGTAGAAATCAGACCAATTTCTGTTTATCTACAGAAAACCAGAAAATGAATTACTTCTACTAGGGCTCGGCCTGGGCAGCCATGGCTGGCCTAATTTTTTTGTTTTAGTGATAATGTGCCTCAAATATTAGGCTTCTGTGTGGCTATCCTATCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTAT
ATATTTGATACTGATGATATGGCATATTCATCCATTGTATTTTGCTCCAAACTCTGTGACTTCACAGCCCTTTTGTTATGGTTCTTGTTATATATTTGATACTGATGATATGGCATATTCATCCATTGTACTTTGCTCTGTATATCAGTCGCTGGGTTTCCTTCGCTTGGTTGAGGTTGGATACAGTTCTTCCATTTAAC
TAAAGTTGC

>TRINITY_DN44172_c0_g1_i6 from sample A7 / Example unspliced isoform:

ACCATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGGC
ATAGGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCATGGGTTTCAAGTAGCTGTTCACCCACAGGTTAA
GGCTTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCAGTGATCGGTGAGACAAAAAGAAAGAAGATATC
GAATTAACTTTCCGTGCGTATATCATTATATTAACTTTCGGCTGATAGATTCGTTCAGTTGCTTTCTACCTTCAGATGTTGTTCTATGGCCCGTTATTTTGTAGAGTGGTTTACCCATTTTTAAAACTTGTTCGTCTGAGCTTTCTTGCCCCTAGATTTTTCACTTCTTCCTGCAATCACACACTGCGAACGGTGAGAGG
GTAACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAGgtgtgtaggtactcttcccggcctttgtgtaggaatcagaaattcagttttctatgttttctgtgtttcttttttcttttcgacagggataggatcggtgtttctgttttactgagaaaaaataggatttaatgttctttctttgttggctg
tgattatcagTTTGTAGATTCCGAAGGGCT

Intron 5 GTGAGACAAA ... TTGCCCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.73	17.6	14	7.2
A2	39.23	11.7	12.9	2.2
A3	28.09	13.3	4.9	5.6
A4	56.18	7.9	14	5
A5	46.89	11	7.4	11.9
A6	41.16	89.4	66.9	58.3
A7	40.1	118.6	106	52.3
A8	46.27	81.6	77.3	61.7
A9	36.12	95.3	72.4	35.5
A10	43.72	111.7	105.3	67.1

>TRINITY_DN113574_c0_g1_i4 from sample A1 / Example spliced isoform

ATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGGCATA
GGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCATGGGTTTCAAGTAGCTGTTCACCCACAGGTTAAGGC
TTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCAGTGATCGATTTTTCACTTCTTCCTGCAATCACACA
CTGCGAACGGTGAGAGGGTAACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAGTTTGTAGATTCCGAAGGGCTAAGGAGGATTGTACTCGTTCGCAAGAACTCTCAAACCGCTATCACTTTCGATTGTTTTACCAACCATGTCTGGGGTGTGTTTGACATAAGCAATAAGGTACGTCTGAAATATCTT
AGAGACGGAGAAATAATAGTGGTAGATGATGAGGTGGGGTGGGAAGAGACCTCCATGAGTTTAAAGAACCAAGAAACCCTCAACGTCGTTCTTGAACGTTTGAATTTTGATGTGAAACTTTTGGAATGTGATGATTACAAGGGAGAGTGGGAACAGATCGGCTTGAATTCATTGAATTCATTTACAACTGTATGAGGAGA
GATCCCTAGTTTTCTCTCACAGTAGAAATCAGACCAATTTCTGTTTATCTACAGAAAACCAGAAAATGAATTACTTCTACTAGGGCTCGGCCTGGGCAGCCATGGCTGGCCTAATTTTTTTGTTTTAGTGATAATGTGCCTCAAATATTAGGCTTCTGTGTGGCTATCCTATCCAAACTCTGTGACTTCACAGCCCTTTT
GTTATGGTTCTTGTTATATATTTGATACTGATGATATGGCATATTCATCCATTGTATTTTGCTCCAAACTCTGTGACTTCACAGCCCTTTTGTTATG

>TRINITY_DN113574_c0_g1_i6 from sample A1 / Example unspliced isoform:

ATTTCCTCTACCTAGAATTCCCAATGCCTTCCAACATTTCCTCTACCTAGAATTCCCACGGGAGAGATCATTGCATTTGGACCTAGATGGGGGCATAGGAGAAGTCCTCTTTGTCACTGTTGGATCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGGCATA
GGAGAAGTCCTCTTTGCCACTGTTGGACCTGTAAGTGTAGTATCATAGATGGCGGCCAACGAAGTGAAGAAGTACAATGCATTTCCAAAAATGTGTTGCCTGTGCACCGGTGAATGCCAGTATGAAGAATCCTTTAGATCCAAGCTTGAGTTCAACAATACCCATGGGTTTCAAGTAGCTGTTCACCCACAGGTTAAGGC
TTTTTCAGATGGCCAGATCCCCAGGTACTCTTGATGATTGCGGGTTGCTCCCTGTTCGTAACGCCATTCTTAATGGAGAAACAAAATATTTGCTGCCTGCTCCGTATGGGGATCGCTTCCTTTGGAACCCTCCGGCCGCGCGCCTGTTCATCATACTCCCATTCAGTGATCGgtgagacaaaaagaaagaagatatcgaa
ttaactttccgtgcgtatatcattatattaactttcggctgatagattcgttcagttgctttctaccttcagatgttgttctatggcccgttattttgtagagtggtttacccatttttaaaacttgttcgtctgagctttcttgcccctagATTTTTCACTTCTTCCTGCAATCACACACTGCGAACGGTGAGAGGGTA
ACGAGAGGCAAACAAGGTAGCGCTATGGATCTGGGACGGATAAAG