sp|Q8L539|5FCL_ARATH ----> 5-formyltetrahydrofolate cyclo-ligase, mitochondrial

Intron 1 GTAATTGGTA ... AACATGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.69	23	6	0.4
A2	11.11	24	3	3.4
A3	44.19	24	19	3.2
A4	11.64	22.8	3	0.4
A5	18.92	30	7	0
A6	20.09	200.8	50.5	3.4
A7	17.64	130.7	28	1
A8	10.35	155.9	18	5.2
A9	13.38	174.8	27	3.6
A10	14.73	110	19	2.2

>TRINITY_DN107687_c0_g1_i5 from sample A3 / Example spliced isoform

CATTTGCAGATATATGTGAAATCACAGCATGGATAAGTGAAAATGGTTAGCAGGTTCGAGAATGCATTTTCCACTACTTACTTTGTATTTTTGAAAGTTGAGCAGGTGATATGCAGATCCCAAAGAAGCTCTATGTTCCTCGTGTTGAAGACAAGGAAAGTAATATGAGAATGTTGCATATTACTAGTAGTGCAGACTTT
GTTGCCAATTCAATGAATATATTGGAGCCAACACCATTGGATAATAGTGGAAATCAACGAGAAAATGTTATGCAGGCAAATGAACCAGTAGATTTGCTTCTCTTGCCTGGGCTTGCCTTTGATCGCTCGGGGAGACGCTTGGGTCGTGGTGGAGGATATTATGATCTTTTTCTCAAAAATTACCTGGAGCTCGCTGGAGA
AAGAGGCTGGCAGCCACCTCTACTAGTTGCATTGACATACTCTACACAAATATTGGACGAACCAATCCCTGTTTCTCCACATGATGTTCTGGTAGATGCTCTTGTTTCACCATCTGGTGTGTTCACGATAAGCCCAAATGCATTGAAAAGAATGTGATCTCAAATAATAGTACCGACTCCTTTGCTGGTCAAAGAGAGAC
TTCAAGGAAATGGCTTCTAATATCTTATGGGAAATTTCCTGCATTTGAAGCTGACTGCAAGTTTTCCCAAGCTAATGGAAATTGTATTCATTATGAGTGTTGGATTATGTATTTGTAAAAGGATCCTCTTCATCAATGCAGATGTAGCTGAATGCTTCATCCAATCAAAAGAACAAATCTGATCACATGACGAATAACTA
TGAACATGGCTCTCAGTGGCCTACAATTGAATAGATTTGTTGTATGACAAAG

>TRINITY_DN107687_c0_g1_i1 from sample A3 / Example unspliced isoform:

GATATGCAGATCCCAAAGAAGCTCTATGTTCCTCGTGTTGAAGACAAGGAAAGTAATATGAGAATGTTGCATATTACTAGTAGTGCAGACTTTGTTGCCAATTCAATGAATATATTGGAGCCAACACCATTGGATAATAGTGGAAATCAACGAGAAAATGTTATGCAGGCAAATGAACCAGTAGATTTGCTTCTCTTGCC
TGgtaattggtactttacactaaagattgacctgaatttttcctttttctaaaaatcatcctgagaatagcatagttatctagatcagatgtttaaaagtagcaaacaaacagtggaaatagtgaagactgaagggagtaaacatataatatatgtgattgaactcctaatctacttttttcctttttcaattcaacatg
gaagGGCTTGCCTTTGATCGCTCGGGGAGACGCTTGGGTCGTGGTGGAGGGTAAGCTTAATCGTGATCTTAATTCTTAGTAGTTTCACTTCCGATGCTAGTTGAAGCAAACCATAGATATATTAGAGGGTTGCTGATTGATTCTTTAATTTGATGGGGA

Intron 2 GTAAGCTTAA ... CTCATGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40	51	1	34
A2	40	51	4	34
A3	36.84	36	2	21
A4	35.71	27	0	15
A5	30.91	38	0	17
A6	36.13	320	0	181
A7	39.92	283	3	188
A8	35.49	289	3	159
A9	33.41	285	0	143
A10	40.68	226	0	155

>TRINITY_DN38246_c0_g1_i1 from sample A8 / Example spliced isoform

TTCCAACTACATAAAACGCCAAAACAACTCCAAAAAAACCCTTAATGAATAAAAATGCCAAAACAACTCGCAAAAAATAAAACCTTAATGAATGACGAAAAATCCCCATGGCAGACCAGGGTTTAAACAAATGTATTTATCAACTTTGCCCATTAACTAAGTAAATGAAGTAACCCTTCAACGATCTTACCGACCGTAAC
CCAAAACCTCAATTTGTAACAGTGGCTACCACCCAGTGACCCACGTCTCAGCCCGAGAGCGCACTACGGTAGCAAGAAAGACGAACAATTTATCGTCCTCAAATAGGAGTTTCGGACATTAAGTCAAATGAAATGCTGATCAATTTGTTTCTCCCCTCAAGCGTTCACTTTCTGAGGACTCTGGTCAACAAACCCTTCTT
GTCCATAAGAATGGCAACGCAGACAACAGCGGCCTCCGATTCCATTTTCCATAGAAAGCGCGCCCTTCGATTCAGAGTGCGCAAGGAGCTCAAGACATTAGATCCCATTCAAAGGCAGCAAGAAGGATCAAGTTTGTAGATTATATGTATGTTCTTGTTGATGATTTTAGATGAAGCTGTCCAAGAGCTGGTCCTTGACG
CACCATGGTTCAAATCCTGCAGTCGGTTGTGTGCATATATAAGCTGCGAGGGTCTAAGAGAAGTCAATACTTCAAAAATAATCTCTAACATTCTGGACAAGCCTAATTCTGGTGATATGCAGATCCCAAAGAAGCTCTATGTTCCTCGTGTTGAAGACAAGGAAAGTAATATGAGAATGTTGCATATTACTAGTAGTGCA
GACTTTGTTGCCAATTCAATGAATATATTGGAGCCAACACCATTGGATAATAGTGGAAATCAACGAGAAAATGTTATGCAGGCAAATGAACCAGTAGATTTGCTTCTCTTGCCTGGGCTTGCCTTTGATCGCTCGGGGAGACGCTTGGGTCGTGGTGGAGGATATTATGATCTTTTTCTCAAAAATTACCTGGAGCTCGC
TGGAGAAAGAGGCTGGCAGCCACCTCTACTAGTTGCATTGACATACTCTACACAAATATTGGACGAACCAATCCCTGTTTCTCCACATGATGTTCTGGTAGATGCTCTTGTTTCACCATCTGGTGTGTTCACGATAAGCCCAAATGCATTGAAAAGAATGTGATCTCAAATAATAGTACCGACTCCTTTGCTGGTCAAAG
AGAGACTTCAAGGAAATGGCTTCTAATATCTTATGGGAAATTTCCTGCATTTGAAGCTGACTGCAAGTTTTCCCAAGCTAATGGAAATTGTATTCATTATGAGTGTTGGATTATGTATTTGTAAAAGGATCCTCTTCATCAATGCAGATGTAGCTGAATGCTTCATCCAATCAAAAGAACAAATCTGATCACATGACGAA
TAACTATGAACATGGCTCTCAGTGGCCTACAATTGAATAGATTTGTTGTATGACAAAGTAGTCATTTTTATTTTCGATTATATTTTTCACATCTTTAAATCAGATTGTGCCATTCAAAATATTTTTGTCAATTTTGAATATTACTTGTAGTTTCTGCACACCACTTCAGTGCCTTTTTTTCCTCAG

>TRINITY_DN38246_c0_g1_i4 from sample A8 / Example unspliced isoform:

TTCCAACTACATAAAACGCCAAAACAACTCCAAAAAAACCCTTAATGAATAAAAATGCCAAAACAACTCGCAAAAAATAAAACCTTAATGAATGACGAAAAATCCCCATGGCAGACCAGGGTTTAAACAAATGTATTTATCAACTTTGCCCATTAACTAAGTAAATGAAGTAACCCTTCAACGATCTTACCGACCGTAAC
CCAAAACCTCAATTTGTAACAGTGGCTACCACCCAGTGACCCACGTCTCAGCCCGAGAGCGCACTACGGTAGCAAGAAAGACGAACAATTTATCGTCCTCAAATAGGAGTTTCGGACATTAAGTCAAATGAAATGCTGATCAATTTGTTTCTCCCCTCAAGCGTTCACTTTCTGAGGACTCTGGTCAACAAACCCTTCTT
GTCCATAAGAATGGCAACGCAGACAACAGCGGCCTCCGATTCCATTTTCCATAGAAAGCGCGCCCTTCGATTCAGAGTGCGCAAGGAGCTCAAGACATTAGATCCCATTCAAAGGCAGCAAGAAGATGAAGCTGTCCAAGAGCTGGTCCTTGACGCACCATGGTTCAAATCCTGCAGTCGGTTGTGTGCATATATAAGCT
GCGAGGGTCTAAGAGAAGTCAATACTTCAAAAATAATCTCTAACATTCTGGACAAGCCTAATTCTGGTGATATGCAGATCCCAAAGAAGCTCTATGTTCCTCGTGTTGAAGACAAGGAAAGTAATATGAGAATGTTGCATATTACTAGTAGTGCAGACTTTGTTGCCAATTCAATGAATATATTGGAGCCAACACCATTG
GATAATAGTGGAAATCAACGAGAAAATGTTATGCAGGCAAATGAACCAGTAGATTTGCTTCTCTTGCCTGGTAATTGGTACTTTACACTAAAGATTGACCTGAATTTTTCCTTTTTCTAAAAATCATCCTGAGAATAGCATAGTTATCTAGATCAGATGTTTAAAAGTAGCAAACAAACAGTGGAAATAGTGAAGACTGA
AGGGAGTAAACATATAATATATGTGATTGAACTCCTAATCTACTTTTTTCCTTTTTCAATTCAACATGGAAGGGCTTGCCTTTGATCGCTCGGGGAGACGCTTGGGTCGTGGTGGAGGgtaagcttaatcgtgatcttaattcttagtagtttcacttccgatgctagttgaagcaaaccatagatatattagagggttg
ctgattgattctttaattttatggggaattctgacagtaaatttttgattggatatgtctcatggaagATATTATGATCTTTTTCTCAAAAATTACCTGGAGCTCGCTGGAGAAAGAGGCTGGCAGCCACCTCTACTAGTTGCATTGACATACTCTACACAAATATTGGACGAACCAATCCCTGTTTCTCCACATGATGT
TCTGGTAGATGCTCTTGTTTCACCATCTGGTGTGTTCACGATAAGCCCAAATGCATTGAAAAGAATGTGATCTCAAATAATAGTACCGACTCCTTTGCTGGTCAAAGAGAGACTTCAAGGAAATGGCTTCTAATATCTTATGGGAAATTTCCTGCATTTGAAGCTGACTGCAAGTTTTCCCAAGCTAATGGAAATTGTAT
TCATTATGAGTGTTGGATTATGTATTTGTAAAAGGATCCTCTTCATCAATGCAGATGTAGCTGAATGCTTCATCCAATCAAAAGAACAAATCTGATCACATGACGAATAACTATGAACATGGCTCTCAGTGGCCTACAATTGAATAGATTTGTTGTATGACAAAGTAGTCATTTTTATTTTCGATTATATTTTTCACATC
TTTAAATCAGATTGTGCCATTCAAAATATTTTTGTCAATTTTGAATATTACTTGTAGTTTCTGCACACCACTTCAGTGCCTTTTTTTCCTCAG

Intron 3 GTAGGAATAT ... GGTTCTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	6.74	83	6	0
A2	1.19	83	1	0
A3	6.98	80	6	0
A4	3.39	57	2	0
A5	5.41	35	2	0
A6	3.6	563	21	0
A7	3.39	513	18	0
A8	3.39	484	17	0
A9	4.25	428	19	0
A10	3.51	385	14	2

>TRINITY_DN44232_c0_g1_i1 from sample A10 / Example spliced isoform

CTTCAACGATCTTACCGACCGTAACCCAAAACCTCAATTTGTAACAGTGGCTACCACCCAGTGACCCACGTCTCAGCCCGAGAGCGCACTACGGTAGCAAGAAAGACGAACAATTTATCGTCCTCAAATAGGAGTTTCGGACATTAAGTCAAATGAAATGCTGATCAATTTGTTTCTCCCCTCAAGCGTTCACTTTCTGA
GGACTCTGGTCAACAAACCCTTCTTGTCCATAAGAATGGCAACGCAGACAACAGCGGCCTCCGATTCCATTTTCCATAGAAAGCGCGCCCTTCGATTCAGAGTGCGCAAGGAGCTCAAGACATTAGATCCCATTCAAAGGCAGCAAGAAGATGAAGCTGTCCAAGAGCTGGTCCTTGACGCACCATGGTTCAAATCCTGC
AGTCGGTTGTGTGCATATATAAGCTGCGAGGGTCTAAGAGAAGTCAATACTTCAAAAATAATCTCTAACATTCTGGACAAGCCTAATTCTGGTGATATGCAGATCCCAAAGAAGCTCTATGTTCCTCGTGTTGAAGACAAGGAAAGTAATATGAGAATGTTGCATATTACTAGTAGTGCAGACTTTGTTGCCAATTCAAT
GAATATATTGGAGCCAACACCATTGGATAATAGTGGAAATCAACGAGAAAATGTTATGCAGGCAAATGAACCAGTAGATTTGCTTCTCTTGCCTGGGCTTGCCTTTGATCGCTCGGGGAGACGCTTGGGTCGTGGTGGAGGATATTATGATCTTTTTCTCAAAAATTACCTGGAGCTCGCTGGAGAAAGAGGCTGGCAGC
CACCTCTACTAGTTGCATTGACATACTCTACACAAATATTGGACGAACCAATCCCTGTTTCTCCACATGATGTTCTGGTAGATGCTCTTGTTTCACCATCTGGTGTGTTCACGATAAGCCCAAATGCATTGAAAAGAATGTGATCTCAAATAATAGTACCGACTCCTTTGCTGGTCAAAGAGAGACTTCAAGGAAATGGC
TTCTAATATCTTATGGGAAATTTCCTGCATTTGAAGCTGACTGCAAGTTTTCCCAAGCTAATGGAAATTGTATTCATTATGAGTGTTGGATTATGTATTTGTAAAAGGATCCTCTTCATCAATGCAGATGTAGCTGAATGCTTCATCCAATCAAAAGAACAAATCTGATCACATGACGAATAACTATGAACATGGCTCTC
AGTGGCCTACAATTGAATAGATTTGTTGTATGACAAAGTAGTCATTTTTATTTTCGATTATATTTTTCACATCTTTAAATCAGATTGTGCCATTCAAAATATTCTTGTCAATTTTGAATATTACTTGTAGTTTCTGCACACCACTTCAG

>TRINITY_DN44232_c0_g1_i3 from sample A10 / Example unspliced isoform:

ATGAAGCTGTCCAAGAGCTGGTCCTTGACGCACCATGGTTCAAATCCTGCAGTCGGTTGTGTGCATATATAAGCTGCGAGGGTCTAAGAGAAGTCAATACTTCAAAAATAATCTCTAACATTCTGGACAAGCCTAATTCTGGTGATATGCAGATCCCAAAGAAGCTCTATGTTCCTCGTGTTGAAGACAAGGAAAGTAAT
ATGAGAATGTTGCATATTACTAGTAGTGCAGACTTTGTTGCCAATTCAATGAATATATTGGAGCCAACACCATTGGATAATAGTGGAAATCAACGAGAAAATGTTATGCAGGCAAATGAACCAGTAGATTTGCTTCTCTTGCCTGGTAATTGGTACTTTACACTAAAGATTGACCTGAATTTTTCCTTTTTCTAAAAATC
ATCCTGAGAATAGCATAGTTATCTAGATCAGATGTTTAAAAGTAGCAAACAAACAGTGGAAATAGTGAAGACTGAAGGGAGTAAACATATAATATATGTGATTGAACTCCTAATCTACTTTTTTCCTTTTTCAATTCAACATGGAAGGGCTTGCCTTTGATCGCTCGGGGAGACGCTTGGGTCGTGGTGGAGGATATTAT
GATCTTTTTCTCAAAAATTACCTGGAGCTCGCTGGAGAAAGAGGCTGGCAGCCACCTCTACTAGgtaggaatatatgagttagtgatggaagaaaatatttcatttctttctaatgtattcatggctgttcttgtaacctttctctatttggttctgtagTTGCATTGACATACTCT