NA ----> NA

Intron 1 GTGATCATTA ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	90.91	1	2	10
A2	75	4	6	12
A3	100	0	2	5
A4	100	0	1	5
A5	100	0	1	3
A6	90.91	5	3	50
A7	83.96	17	18	89
A8	88.42	11	31	84
A9	91.67	3	18	33
A10	84.21	9	17	48

>TRINITY_DN48601_c0_g2_i12 from sample A10 / Example spliced isoform

AACAAACACATTTTACTGCGAGAATTCATGAATATTAAAAATCGCCCAATTGCTGCCAGTTTGCCATCACAATATATTACGGAGTTGCTCTATTCGAGATTTATTGAATTCATTTGATCGCTCTAATTTCCAGCAAATCCCCTTATTTTCTACCAGATCATCAGTGAAGGAAAACACCTGGCCACAAAAACGCAATTGTA
AAGATCTTGATAGAGTTTTGCCAGAAAGATAACGATTGATTGCCATGCAGAAGGAGAAGGAGATACAAAACGTTCGATGGCGAAGTAGTGAAAATGCGTTGGAATAATTTCCTGGAAGATAGCATGAAAATGGTCTCGGATCAATCTACACGAGAATGGTCATGTTAATAATTCACACACTATTTACCGTGGATTCAAGG
TTCGATGAAGAAAGGAATGGTCAGTTTCTCGATAACGGAATGTCTTTTGAAATGTGCCACGATGGAGAGATATTTTCAAATCGCTCAAAACAAGCAAATCTTGGAGCCTGAAGTCAAGAAGAAATAACTTATTCTGTTCTACCCGCTACAGTAGGATGAGGAGTCTGTTATTTGTCCTTCTGGTCTATATAAACACAGAG
TTCCATTGTACAGGGGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTCAATTCTGTCTCTGCTTGTAAATCTTGATATTTGCAGATAGAACGAGAGATT

>TRINITY_DN48601_c0_g2_i2 from sample A10 / Example unspliced isoform:

AACAAACACATTTTACTGCGAGAATTCATGAATATTAAAAATCGCCCAATTGCTGCCAGTTTGCCATCACAATATATTACGGAGTTGCTCTATTCGAGATTTATTGAATTCATTTGATCGCTCTAATTTCCAGCAAATCCCCTTATTTTCTACCAGATCATCAGTGAAGGAAAACACCTGGCCACAAAAACGCAATTGTA
AAGATCTTGATAGAGTTTTGCCAGAAAGATAACGATTGATTGCCATGCAGAAGGAGAAGGAGATACAAAACgtgatcattacatcaatgcatctggtttttaattagttatcgggatttgaggagtttagttatgtagaatcgccagcaattgattttttttggatttttgtggcgaacgtggacttatattgacatgaa
agtatcattttattaattatatgaaagtatccttttgacaggtaagatcccgaaagcatctgatatgcaaggatcgttaagtttgttttaacaaaattagcgagattgcagtcccatcttcagttgaaacttaaagattgttaaagagtgtttaatcacaattgctggaaaagaggaacgcaacgaatagatctcgatgg
aagatcataggtggcatcattaggaaaagtcatgcaatatcaataatatgggagtttacgaatgaattgtaaataacatgctgtaaaatttcttatgaagtgcattttaccaaacagaagcctgcacctattactgcatacgatttgcccgaattttcttttctagcgtgacattcagaaataaatgatcattttaccaa
caaattgatcgcataagaacgaagaaaatgctgagtatatcaagcaattgtcggaaatttactataagggaagtaccttatctatttatttctattattttatctttcaagataaattcatttatttcccttctttcttattttgcagGTTCGATGGCGAAGTAGTGAAAATGCGTTGGAATAATTTCCTGGAAGATAGC
ATGAAAATGGTCTCGGATCAATCTACACGAGAATGGTCATGTTAATAATTCACACACTATTTACCGTGGATTCAAGGTTCGATGAAGAAAGGAATGGTCAGTTTCTCGATAACGGAATGTCTTTTGAAATGTGCCACGATGGAGAGATATTTTCAAATCGCTCAAAACAAGCAAATCTTGGAGCCTGAAGTCAAGAAGAA
ATAACTTATTCTGTTCTACCCGCTACAGTAGGATGAGGAGTCTGTTATTTGTCCTTCTGGTCTATATAAACACAGAGTTCCATTGTACAGGGGGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTCAATTCTGTCTCTGCTTGTAAATCTTGATATTTGCAGATAGAACGAGAGATT

Intron 2 GTGATCATTA ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	90.91	1	2	10
A2	75	4	4	12
A3	100	0	2	5
A4	100	0	0	5
A5	100	0	1	3
A6	90.91	5	1	50
A7	83.88	17	16	88.5
A8	88.54	11	18	85
A9	91.67	3	5	33
A10	84.21	9	16	48

>TRINITY_DN48601_c0_g2_i12 from sample A10 / Example spliced isoform

AACAAACACATTTTACTGCGAGAATTCATGAATATTAAAAATCGCCCAATTGCTGCCAGTTTGCCATCACAATATATTACGGAGTTGCTCTATTCGAGATTTATTGAATTCATTTGATCGCTCTAATTTCCAGCAAATCCCCTTATTTTCTACCAGATCATCAGTGAAGGAAAACACCTGGCCACAAAAACGCAATTGTA
AAGATCTTGATAGAGTTTTGCCAGAAAGATAACGATTGATTGCCATGCAGAAGGAGAAGGAGATACAAAACGTTCGATGGCGAAGTAGTGAAAATGCGTTGGAATAATTTCCTGGAAGATAGCATGAAAATGGTCTCGGATCAATCTACACGAGAATGGTCATGTTAATAATTCACACACTATTTACCGTGGATTCAAGG
TTCGATGAAGAAAGGAATGGTCAGTTTCTCGATAACGGAATGTCTTTTGAAATGTGCCACGATGGAGAGATATTTTCAAATCGCTCAAAACAAGCAAATCTTGGAGCCTGAAGTCAAGAAGAAATAACTTATTCTGTTCTACCCGCTACAGTAGGATGAGGAGTCTGTTATTTGTCCTTCTGGTCTATATAAACACAGAG
TTCCATTGTACAGGGGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTCAATTCTGTCTCTGCTTGTAAATCTTGATATTTGCAGATAGAACGAGAGATT

>TRINITY_DN48601_c0_g2_i11 from sample A10 / Example unspliced isoform:

AACAAACACATTTTACTGCGAGAATTCATGAATATTAAAAATCGCCCAATTGCTGCCAGTTTGCCATCACAATATATTACGGAGTTGCTCTATTCGAGATTTATTGAATTCATTTGATCGCTCTAATTTCCAGCAAATCCCCTTATTTTCTACCAGATCATCAGTGAAGGAAAACACCTGGCCACAAAAACGCAATTGTA
AAGATCTTGATAGAGTTTTGCCAGAAAGATAACGATTGATTGCCATGCAGAAGGAGAAGGAGATACAAAACgtgatcattacatcaatgcatctggtttttaattagttatcgggatttgaggagtttagtaagatcccgaaagcatctgatatgcaaggatcgttaagtttgttttaacaaaattagcgagattgcagt
cccatcttcagttgaaacttaaagattgttaaagagtgtttaatcacaattgctggaaaagaggaacgcaacgaatagatctcgatggaagatcataggtggcatcattaggaaaagtcatgcaatatcaataatatgggagtttacgaatgaattgtaaataacatgctgtaaaatttcttatgaagtgcattttacca
aacagaagcctgcacctattactgcatacgatttgcccgaattttcttttctagcgtgacattcagaaataaatgatcattttaccaacaaattgatcgcatcagaacgaagaaaatgctgagtatatcaagcaattgtcggaaatttactataagggaagtaccttatctatttatttctattattttatctttcaaga
taaattcatttatttcccttctttcttattttgcagGTTCGATGGCGAAGTAGTGAAAATGCGTTGGAATAATTTCCTGGAAGATAGCATGAAAATGGTCTCGGATCAATCTACACGAGAATGGTGAATAAAGGATCGTCATGTTAATAATTATCTCACTATTTACCGTGGATTCAAGGTTCGATGAAGAAAGGAATGGT
CAGTTTCTCGATAACGGAATGTCTTTTGAAATGTGCCACGATGGAGATGGAGAGACATTTTCAAATCGCTCAAAACAAGCAAATCTTGGAGCCTGAAGTCAAGAAGAAATAACTTATTCTGTTCTACCCGCTACAGTAGGATGAGGAGTCTGTTATTTGTCCTTCTGGTCTATATAAACACAGAGTTCCATTGTACAGGG
GGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTCAATTCTGTCTCTGCTTGTAAATCTTGATATTTGCAGATAGAACGAGAGATT

Intron 3 GTAAATAACA ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	0	9
A2	87.5	1	5	7
A3	100	0	2	2
A4	100	0	1	5
A5	100	0	0	3
A6	100	0	6	32
A7	93.1	4	12	54
A8	98.18	1	7	54
A9	95.65	1	6	22
A10	100	0	5	32

>TRINITY_DN49023_c0_g2_i8 from sample A7 / Example spliced isoform

TGAGGATTCATTACACCAGATAGTTAAGGGTTTTCCAGTACATTGGTTTGTTTCAAACTTATAGTGCATGCCGTTAACATCAAATGCCACGTACTGTCTGTGTTTGGATGGTGCCATGATATGCTGCTACATTGCTGGAGATTGTTCGATTTTCCATAAAGAAGCTCGCATCACAGGTATCTCACAAATGATGACCTGTG
CCAAATCCGAAGCATGAAATCATGCAGAGTTGGCCCAGATTCGCCGCAACAATGACACCAAGTTAATGCTGTGGTTTGATCACTTATTGTTAACTGCCAGCGTAAGATCCCCAAAGCATCTGAGCTACAAGGATCGTTAAATTTGTTTTAACAAAATTAGCGAGATTGCAATCCAATCTTCAGTTGAAACTTAAAGATTG
TTAAAGAGTGTTTAATCACAATTGCTGGAAAAGAAAAGAGGAATCTCGATGGAAGATCGTAGGTGGCATTATCTGGAAAAGTCAAAGTCAGGCAATATCAATAGTATGGGATTTTACGAATGAATTGTTCGATGGCGAAGTAGTGA

>TRINITY_DN49023_c0_g2_i7 from sample A7 / Example unspliced isoform:

TGAGGATTCATTACACCAGATAGTTAAGGGTTTTCCAGTACATTGGTTTGTTTCAAACTTATAGTGCATGCCGTTAACATCAAATGCCACGTACTGTCTGTGTTTGGATGGTGCCATGATATGCTGCTACATTGCTGGAGATTGTTCGATTTTCCATAAAGAAGCTCGCATCACAGGTATCTCACAAATGATGACCTGTG
CCAAATCCGAAGCATGAAATCATGCAGAGTTGGCCCAGATTCGCCGCAACAATGACACCAAGTTAATGCTGTGGTTTGATCACTTATTGTTAACTGCCAGCGTAAGATCCCCAAAGCATCTGAGCTACAAGGATCGTTAAATTTGTTTTAACAAAATTAGCGAGATTGCAATCCAATCTTCAGTTGAAACTTAAAGATTG
TTAAAGAGTGTTTAATCACAATTGCTGGAAAAGAAAAGAGGAATCTCGATGGAAGATCGTAGGTGGCATTATCTGGAAAAGTCAAAGTCAGGCAATATCAATAGTATGGGATTTTACGAATGAATTgtaaataacatgctgtaaaatttcttatgaagtgcattttaccaaacacgatttgcccgaattttcttttgtag
cgtgacatttagaaacaaatgatcattttaccaacaaattgatcgcatcagaacgaagaaaatgctgagtatatcaagcaattgtcggaaatttactataagggaagtaccttatctatttatttctattattttatctttcaagataaattcatttatttcccttctttcttattttgcagGTTCGATGGCGAAGTGGT
GAAAATGCGTTGGAATAATTTCCTGGAAGATAGCAT

Intron 4 GTATGGGATT ... TCTTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40	3	1	3
A2	52.38	5	5	6
A3	100	0	2	1
A4	86.67	1	1	12
A5	100	0	0	1
A6	58.62	6	5	12
A7	64.71	9	15	18
A8	65.38	9	8	26
A9	60	5	5	10
A10	52.94	12	7	20

>TRINITY_DN48601_c0_g2_i4 from sample A10 / Example spliced isoform

TGAGGATTCATTACACCAGATAGTTAAGGGTTTTCCAGTACATTGGTTTGTTTCAAACTTATAGTGCATGCCGTTAACATCAAATGCCACGTACTGTCTGTGTTTGGATGGTGCCATGATATGCTGCTACATTGCTGGAGATTGTTCGATTTTCCATAAAGAAGCTCGCATCACAGGTATCTCACAAATGATGACCTGTG
CCAAATCCGAAGCATGAAATCATGCAGAGTTGGCCCAGATTCGCCGCAACAATGACACCAAGTTAATGCTGTGGTTTGATCACTTATTGTTAACTGCCAGCGTAAGATCCCCAAAGCATCTGAGCTACAAGGATCGTTAAATTTGTTTTAACAAAATTAGCGAGATTGCAATCCAATCTTCAGTTGAAACTTAAAGATTG
TTAAAGAGTGTTTAATCACAATTGCTGGAAAAGAAAAGAGGAATCTCGATGGAAGATCGTAGGTGGCATTATCTGGAAAAGTCAAAGTCAGGCAATATCAATACGTGACATTTAGAAACAAATGATCATTTTACCAGCAAATTGATCGCATCAGAACGAAGAAAATGCTGAGTATATCAAGCAATTGTCGGAAATTTACT
ATAAGGGAAGTACCTTATCTATTTATTTCTATTATTTTATCTTTCAAGATAAATTCATTTATTTCCCTTCTTTCTTATTTTGCAGGT

>TRINITY_DN48601_c0_g2_i7 from sample A10 / Example unspliced isoform:

TGAGGATTCATTACACCAGATAGTTAAGGGTTTTCCAGTACATTGGTTTGTTTCAAACTTATAGTGCATGCCGTTAACATCAAATGCCACGTACTGTCTGTGTTTGGATGGTGCCATGATATGCTGCTACATTGCTGGAGATTGTTCGATTTTCCATAAAGAAGCTCGCATCACAGGTATCTCACAAATGATGACCTGTG
CCAAATCCGAAGCATGAAATCATGCAGAGTTGGCCCAGATTCGCCGCAACAATGACACCAAGTTAATGCTGTGGTTTGATCACTTATTGTTAACTGCCAGCGTAAGATCCCCAAAGCATCTGAGCTACAAGGATCGTTAAATTTGTTTTAACAAAATTAGCGAGATTGCAATCCAATCTTCAGTTGAAACTTAAAGATTG
TTAAAGAGTGTTTAATCACAATTGCTGGAAAAGAAAAGAGGAATCTCGATGGAAGATCGTAGGTGGCATTATCTGGAAAAGTCAAAGTCAGGCAATATCAATAgtatgggattttacgaatgaattgtaaataacatgctgtaaaatttcttatgaagtgcattttaccaaacacgatttgcccgaattttcttttgtag


CGTGACATTTAGAAACAAATGATCATTTTACCAGCAAATTGATCGCATCAGAACGAAGAAAATGCTGAGTATATCAAGCAATTGTCGGAAATTTACTATAAGGGAAGTACCTTATCTATTTATTTCTATTATTTTATCTTTCAAGATAAATTCATTTATTTCCCTTCTTTCTTATTTTGCAGGTTCGATGGCGAAGTAGT
GAAAATGCGTTGGAATAATTTCCTGGAAGATAGCATGAAAATGGTCTCGGATCAATCTACACGAGAATGGTCATGTTAATAATTCACACACTATTTACCGTGGATTCAAGGTTCGATGAAGAAAGGAATGGTCAGTTTCTCGATAACGGAATGTCTTTTGAAATGTGCCACGATGGAGAGATATTTTCAAATCGCTCAAA
ACAAGCAAATCTTGGAGCCTGAAGTCAAGAAGAAATAACTTATTCTGTTCTACCCGCTACAGTAGGATGAGGAGTCTGTTATTTGTCCTTCTGGTCTATATAAACACAGAGTTCCATTGTACAGGGGGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTCAATTCTGTCTCTGCTTGTAAATCTTGATATTTG
CAGATAGAACGAGAGATT

Intron 5 GTAAATAACA ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	1	9
A2	88.89	1	9	7
A3	100	0	6	2
A4	100	0	2	5
A5	100	0	5	3
A6	100	0	23	32
A7	92.31	4	42	54
A8	97.75	1	33	54
A9	95.74	1	23	22
A10	100	0	19	32

>TRINITY_DN49023_c0_g2_i8 from sample A7 / Example spliced isoform

TGAGGATTCATTACACCAGATAGTTAAGGGTTTTCCAGTACATTGGTTTGTTTCAAACTTATAGTGCATGCCGTTAACATCAAATGCCACGTACTGTCTGTGTTTGGATGGTGCCATGATATGCTGCTACATTGCTGGAGATTGTTCGATTTTCCATAAAGAAGCTCGCATCACAGGTATCTCACAAATGATGACCTGTG
CCAAATCCGAAGCATGAAATCATGCAGAGTTGGCCCAGATTCGCCGCAACAATGACACCAAGTTAATGCTGTGGTTTGATCACTTATTGTTAACTGCCAGCGTAAGATCCCCAAAGCATCTGAGCTACAAGGATCGTTAAATTTGTTTTAACAAAATTAGCGAGATTGCAATCCAATCTTCAGTTGAAACTTAAAGATTG
TTAAAGAGTGTTTAATCACAATTGCTGGAAAAGAAAAGAGGAATCTCGATGGAAGATCGTAGGTGGCATTATCTGGAAAAGTCAAAGTCAGGCAATATCAATAGTATGGGATTTTACGAATGAATTGTTCGATGGCGAAGTAGTGA

>TRINITY_DN49023_c0_g2_i6 from sample A7 / Example unspliced isoform:

TGAGGATTCATTACACCAGATAGTTAAGGGTTTTCCAGTACATTGGTTTGTTTCAAACTTATAGTGCATGCCGTTAACATCAAATGCCACGTACTGTCTGTGTTTGGATGGTGCCATGATATGCTGCTACATTGCTGGAGATTGTTCGATTTTCCATAAAGAAGCTCGCATCACAGGTATCTCACAAATGATGACCTGTG
CCAAATCCGAAGCATGAAATCATGCAGAGTTGGCCCAGATTCGCCGCAACAATGACACCAAGTTAATGCTGTGGTTTGATCACTTATTGTTAACTGCCAGCGTAAGATCCCCAAAGCATCTGAGCTACAAGGATCGTTAAATTTGTTTTAACAAAATTAGCGAGATTGCAATCCAATCTTCAGTTGAAACTTAAAGATTG
TTAAAGAGTGTTTAATCACAATTGCTGGAAAAGAAAAGAGGAATCTCGATGGAAGATCGTAGGTGGCATTATCTGGAAAAGTCAAAGTCAGGCAATATCAATAGTATGGGATTTTACGAATGAATTgtaaataacatgctgtaaaatttcttatgaagtgcattttaccaaacagaagcctgcacctattactgcatacg
atttgcccgaattttcttttctagcgtgacattcagaaataaatgatcattttaccaacaaattgatcgcatcagaacgaagaaaatgctgagtatatcaagcaattgtcggaaatttactataagggaagtaccttatctatttatttctattattttatctttcaagataaattcatttatttcccttctttcttatt
ttgcagGTTCGATGGCGAAGTGGTGAAAATGCGTTGGAATAATTTCCTGGAAGATAGCAT

Intron 6 GTAAGATCCC ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.82	7	6	10
A2	92.31	1	5	12
A3	55.56	4	4	5
A4	100	0	0	5
A5	42.86	4	4	3
A6	87.72	7	7	50
A7	85.58	15	19	89
A8	94.38	5	11	84
A9	100	0	2	33
A10	77.42	14	12	48

>TRINITY_DN48601_c0_g2_i5 from sample A10 / Example spliced isoform

TGAGGATTCATTACACCAGATAGTTAAGGGTTTTCCAGTACATTGGTTTGTTTCAAACTTATAGTGCATGCCGTTAACATCAAATGCCACGTACTGTCTGTGTTTGGATGGTGCCATGATATGCTGCTACATTGCTGGAGATTGTTCGATTTTCCATAAAGAAGCTCGCATCACAGGTATCTCACAAATGATGACCTGTG
CCAAATCCGAAGCATGAAATCATGCAGAGTTGGCCCAGATTCGCCGCAACAATGACACCAAGTTAATGCTGTGGTTTGATCACTTATTGTTAACTGCCAGCGTTCGATGGCGAAGTAGTGAAAATGCGTTGGAATAATTTCCTGGAAGATAGCATGAAAATGGATCAAGTTTTACCACATAACAATTTACCCATTGCTCA
GCATATGAAAGTAATGACATCATACGCTCTACCTTCGACAACACGCTGGGCGAGGTTGTAAATTAAAGGGAAGATTT

>TRINITY_DN48601_c0_g2_i7 from sample A10 / Example unspliced isoform:

TGAGGATTCATTACACCAGATAGTTAAGGGTTTTCCAGTACATTGGTTTGTTTCAAACTTATAGTGCATGCCGTTAACATCAAATGCCACGTACTGTCTGTGTTTGGATGGTGCCATGATATGCTGCTACATTGCTGGAGATTGTTCGATTTTCCATAAAGAAGCTCGCATCACAGGTATCTCACAAATGATGACCTGTG
CCAAATCCGAAGCATGAAATCATGCAGAGTTGGCCCAGATTCGCCGCAACAATGACACCAAGTTAATGCTGTGGTTTGATCACTTATTGTTAACTGCCAGCgtaagatccccaaagcatctgagctacaaggatcgttaaatttgttttaacaaaattagcgagattgcaatccaatcttcagttgaaacttaaagattg
ttaaagagtgtttaatcacaattgctggaaaagaaaagaggaatctcgatggaagatcgtaggtggcattatctggaaaagtcaaagtcaggcaatatcaatagtatgggattttacgaatgaattgtaaataacatgctgtaaaatttcttatgaagtgcattttaccaaacacgatttgcccgaattttcttttgtag
cgtgacatttagaaacaaatgatcattttaccagcaaattgatcgcatcagaacgaagaaaatgctgagtatatcaagcaattgtcggaaatttactataagggaagtaccttatctatttatttctattattttatctttcaagataaattcatttatttcccttctttcttattttgcagGTTCGATGGCGAAGTAGT
GAAAATGCGTTGGAATAATTTCCTGGAAGATAGCATGAAAATGGTCTCGGATCAATCTACACGAGAATGGTCATGTTAATAATTCACACACTATTTACCGTGGATTCAAGGTTCGATGAAGAAAGGAATGGTCAGTTTCTCGATAACGGAATGTCTTTTGAAATGTGCCACGATGGAGAGATATTTTCAAATCGCTCAAA
ACAAGCAAATCTTGGAGCCTGAAGTCAAGAAGAAATAACTTATTCTGTTCTACCCGCTACAGTAGGATGAGGAGTCTGTTATTTGTCCTTCTGGTCTATATAAACACAGAGTTCCATTGTACAGGGGGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTCAATTCTGTCTCTGCTTGTAAATCTTGATATTTG
CAGATAGAACGAGAGATT

Intron 7 GTTATGTAGA ... CTTTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	3	4
A2	71.43	1	3	2
A3	33.33	5	2	3
A4	100	0	1	2
A5	71.43	1	2	3
A6	100	0	5	3
A7	71.43	8	19	21
A8	65.63	11	27	15
A9	95.74	1	29	16
A10	50.77	16	17	16

>TRINITY_DN49023_c0_g2_i1 from sample A7 / Example spliced isoform

TGTATGTCACCAATAAAAGGAAATCTAAATCAGATCTCCTGGAAAGGTGCAACAAACACATTTTACTGCGAGAATTCATGAATATTAAAAATCGCCCAATTGCTGCCAGTTTGCCATCACAATATATTACGGAGTTGCTCTATTCGAGATTTATTGAATTCATTTGATCGCTCTAATTTCCAGCAAATCCCCTTATTTTC
TACCAGATCATCAGTGAAGGAAAACACCTGGCCACAAAAACGCAATTGTAAAGATCTTGATAGAGTTTTGCCAGAAAGATAACGATTGATTGCCATGCAGAAGGAGAAGGAGATACAAAACGTGATCATTACATCAATGCATCTGGTTTTTAATTAGTTATCGGGATTTGAGGAGTTTAGTAAGATCCCGAAAGCATCTG
ATATGCAAGGATCGTTAAGTTTGTTTTAACAAAATTAGCGAGATTGCAGTCCCATCTTCAGTTGAAACTTAAAGATTGTTAAAGAGTGTTTAATCACAATTGCTGGAAAAGAGGAACGCAACGAATAGATCTCGATGGAAGATCATAGGTGGCATCATTAGGAAAAGTCATGCAATATCAATAATATGGGAGTTTACGAA
TGAATTGTAAATAACATGCTGTAAAATTTCTTATGAAGTGCATTTTACCAAACAGAAGCCTGCACCTATTACTGCATACGATTTGCCCGAATTTTCTTTTCTAGCGTGACATTCAGAAATAAATGATCATTTTACCAACAAATTGATCGCATAAGAACGAAGAAAATGCTGAGTATATCAAGCAATTGTCGGAAATTTAC
TATAAGGGAAGTACCTTATCTATTTATTTCTATTATTTTATCTTTCAAGATAAATTCATTTATTTCCCTTCTTTCTTATTTTGCAGGTTCGATGGCGAAGTGGTGAAAATGCGTTGGAATAATTTCCTGGAAGATAGCAT

>TRINITY_DN49023_c0_g2_i2 from sample A7 / Example unspliced isoform:

TGTATGTCACCAATAAAAGGAAATCTAAATCAGATCTCCTGGAAAGGTGCAACAAACACATTTTACTGCGAGAATTCATGAATATTAAAAATCGCCCAATTGCTGCCAGTTTGCCATCACAATATATTACGGAGTTGCTCTATTCGAGATTTATTGAATTCATTTGATCGCTCTAATTTCCAGCAAATCCCCTTATTTTC
TACCAGATCATCAGTGAAGGAAAACACCTGGCCACAAAAACGCAATTGTAAAGATCTTGATAGAGTTTTGCCAGAAAGATAACGATTGATTGCCATGCAGAAGGAGAAGGAGATACAAAACGTGATCATTACATCAATGCATCTGGTTTTTAATTAGTTATCGGGATTTGAGGAGTTTAgttatgtagaatcgccagcaa
ttgattttttttggatttttgtggcgaacgtggacttatattgacatgaaagtatcattttattaattatatgaaagtatccttttgacagGTAAGATCCCGAAAGCATCTGATATGCAAGGATCGTTAAGTTTGTTTTAACAAAATTAGCGAGATTGCAGTCCCATCTTCAGTTGAAACTTAAAGATTGTTAAAGAGTG
TTTAATCACAATTGCTGGAAAAGAGGAACGCAACGAATAGATCTCGATGGAAGATCATAGGTGGCATCATTAGGAAAAGTCATGCAATATCAATAATATGGGAGTTTACGAATGAATTGTAAATAACATGCTGTAAAATTTCTTATGAAGTGCATTTTACCAAACAGAAGCCTGCACCTATTACTGCATACGATTTGCCC
GAATTTTCTTTTCTAGCGTGACATTCAGAAATAAATGATCATTTTACCAACAAATTGATCGCATAAGAACGAAGAAAATGCTGAGTATATCAAGCAATTGTCGGAAATTTACTATAAGGGAAGTACCTTATCTATTTATTTCTATTATTTTATCTTTCAAGATAAATTCATTTATTTCCCTTCTTTCTTATTTTGCAGGT
TCGATGGCGAAGTGGTGAAAATGCGTTGGAATAATTTCCTGGAAGATAGCAT

Intron 8 GTAAGATCCC ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.71	3	4	7
A2	75	1	2	4
A3	100	0	3	0
A4	100	0	2	5
A5	55.56	2	3	2
A6	73.33	4	3	19
A7	69.23	14	21	42
A8	93.44	2	15	42
A9	67.44	7	16	13
A10	81.82	5	16	29

>TRINITY_DN44591_c0_g1_i6 from sample A6 / Example spliced isoform

CACATTTTACTGCGAGAATTCATGAATATTAAAAATCGCCCAATTGCTGCCAGTTTGCCATCACAATATATTACGGAGTTGCTCTATTCGAGATTTATTGAATTCATTTGATCGCTCTAATTTCCAGCAAATCCCCTTATTTTCTACCAGATCATCAGTGAAGGAAAACACCTGGCCACAAAAACGCAATTGTAAAGATC
TTGATAGAGTTTTGCCAGAAAGATAACGATTGATTGCCATGCAGAAGGAGAAGGAGATACAAAACGTGATCATTACATCAATGCATCTGGTTTTTAATTAGTTATCGGGATTTGAGGAGTTTAGTTATGTAGAATCGCCAGCAATTGATTTTTTTTGGATTTTTGTGGCGAACGTGGACTTATATTGACATGAAAGTATC
ATTTTATTAATTATATGAAAGTATCCTTTTGACAGGTTCGATGGCGAAGTGGTGAAAATGCTTGGAATCTACCGTGGATTCAAGGATAGCGTTGGAATAATTTCCTGGAAGATAGCATGAAAATGGATGAAAATGGTCTCGGATCAATCTACACGAGAATGGTCATGTTAATAATTCACACACTATTTACCGTGGATTCA
AGGTTCGATGAAGAAAGGAATGGTCAGTTTCTCGATAACGGAATGTCTTTTGAAATGTGCCACGATGGAGAGATATTTTCAAATCGCTCAAAACAAGCAAATCTTGGAGCCTGAAGTCAAGAAGAAATAACTTATTCTGTTCTACCAGCTACAGTAGGATGAGGAGTCTGTTATTTGTCCTTCTGGTCTATATAAACACA
GAGTTCCATTGTACAGGGGGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTCAATTCTGTCTCTGCTTGTAAATCTTGATATTTGCAG

>TRINITY_DN44591_c0_g1_i8 from sample A6 / Example unspliced isoform:

CACATTTTACTGCGAGAATTCATGAATATTAAAAATCGCCCAATTGCTGCCAGTTTGCCATCACAATATATTACGGAGTTGCTCTATTCGAGATTTATTGAATTCATTTGATCGCTCTAATTTCCAGCAAATCCCCTTATTTTCTACCAGATCATCAGTGAAGGAAAACACCTGGCCACAAAAACGCAATTGTAAAGATC
TTGATAGAGTTTTGCCAGAAAGATAACGATTGATTGCCATGCAGAAGGAGAAGGAGATACAAAACGTGATCATTACATCAATGCATCTGGTTTTTAATTAGTTATCGGGATTTGAGGAGTTTAGTTATGTAGAATCGCCAGCAATTGATTTTTTTTGGATTTTTGTGGCGAACGTGGACTTATATTGACATGAAAGTATC
ATTTTATTAATTATATGAAAGTATCCTTTTGACAGgtaagatcccgaaagcatctgatatgcaaggatcgttaagtttgttttaacaaaattagcgagattgcagtcccatcttcagttgaaacttaaagattgttaaagagtgtttaatcacaattgctggaaaagaggaacgcaacgaatagatctcgatggaagatc
ataggtggcatcattaggaaaagtcatgcaatatcaataatatgggagtttacgaatgaattgtaaataacatgctgtaaaatttcttatgaagtgcattttaccaaacagaagcctgcacctattactgcatacgatttgcccgaattttcttttctagcgtgacattcagaaataaatgatcattttaccaacaaatt
gatcgcatcagaacgaagaaaatgctgagtatatcaagcaattgtcggaaatttactataagggaagtaccttatctatttatttctattattttatctttcaagataaattcatttatttcccttctttcttattttgcagGTTCGATGGCGAAGTGGTGAAAATGCTTGGAATCTACCGTGGATTCAAGGATAGCGTT
GGAATAATTTCCTGGAAGATAGCATGAAAATGGATGAAAATGGTCTCGGATCAATCTACACGAGAATGGTCATGTTAATAATTCACACACTATTTACCGTGGATTCAAGGTTCGATGAAGAAAGGAATGGTCAGTTTCTCGATAACGGAATGTCTTTTGAAATGTGCCACGATGGAGAGATATTTTCAAATCGCTCAAAA
CAAGCAAATCTTGGAGCCTGAAGTCAAGAAGAAATAACTTATTCTGTTCTACCAGCTACAGTAGGATGAGGAGTCTGTTATTTGTCCTTCTGGTCTATATAAACACAGAGTTCCATTGTACAGGGGGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTCAATTCTGTCTCTGCTTGTAAATCTTGATATTTGC
AG