sp|Q6YYC0|C3H55_ORYSJ ----> Zinc finger CCCH domain-containing protein 55


Intron 1 GCAAGTGACA ... GCTCTCCGAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A122.86108632
A229.280433
A326.9684231
A428.458323
A525.7649517
A617.783788
A721.01109729
A827.271606
A924.322829
A1023.4775623



>TRINITY_DN104690_c3_g1_i9 from sample A5 / Example spliced isoform
ATCAGAAGCATGGAAGTATGTGAGATGTTTCTTGTTGACCTTCTGATAGTATATTTAAAGCTTTGACTGCCATTTCTAAGAGTTTTAATTTTGGGATATGGCTATTATGTTTTATTGCTTAATTGAATGATTGAATGCTTTTGACCTTGCTTTGGGTTCTCTTGGCTATGGCATGCCATTGTGATGGTCACAAAAAATTC
TATGTCTAGAAGTATTGTTATTTTTTGATTCACTTTCAGACATTGTGGCATTTTAGTGACCATGTCTGGATAATCTGAGCCCAGTCCCATCTTTGCCAGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCA
ATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATGAAAACAGTAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGA
GACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGATGGAGAAGTTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTC
GCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAG
GCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAA
AAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCC
CCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTA
CAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAG
AGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGA
GACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAAT
TCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAAC
ACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTC
ATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGAC
TGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAA
AACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTT
TTTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTT
TGTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGGGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAA
TGGTGTTATATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTATCCTCAGGATAGATATGATGCTTAACTGTGCCCCCAGCTTCTGAGGGTGTGCTCATAAGTTCATTATTTGGT


>TRINITY_DN104690_c3_g1_i3 from sample A5 / Example unspliced isoform:
ATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGgcaagtgacagtgaatcagatgaggtgctgtatagtgcaaatgaattaagaaaaggtcgcagaagcagcaaaagccggtcaccagaaggctc
taggttgagatctcgcaggtttgatgcaaaacctgttcaccctagtttagttctgaggtatcttggcaggcattgtgtgtcattcataaatttgttttattatttttagaacataaacagttattttactaagatctatttttgattagatttatgttcaatatgggggtatgagattattatatatggcatggtatgat
ttaattatctgggaagatccttgatcttcatggtttctttatatttcgaaattgtcacatgtgttactgtgcatgtttccgctctccgagGCAAGGCGGGAGCAGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGT
CGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGA
GAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCG
AAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGG
CACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAA
GATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGA
AGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTC
ATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTAT
AGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATT
TGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAA
GCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAA
GGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGG



Intron 2 GTACTTGTGC ... TTTTCTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A173.0318.339.160
A271.6114.739.733
A367.9219.948.735
A469.516.238.535
A573.727.71528
A688.321.71015
A774.856.21918
A851.072.423
A966.718.311.122
A1050.8715.113.218



>TRINITY_DN131401_c4_g2_i5 from sample A1 / Example spliced isoform
GCCGAAGGTTAGTTTACTTGCATGCTTATGCCATGTTAGGGTTTTAACATTTTTCTTCTGCCGTGCTTTTGCAAGAATGGAATCCACTGCTCTGTTATGTTGTATTCTTATTCCAATTGTTGAAGTCGGCTGCTCTCAGCGGATGTTTTTGGTCAACATTGCCAATCTCACATGATTAGGGCCGGTGCTCCTGCTTGCAT
GCCTTTTGAAGAAACAGTCGGCCCAAGAATGAGAGCAAGATCCATACAATGAGAGAAGACATAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTA
GACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGTTTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTT
GGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCACCTGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTT
TTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTCCTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATT
TGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTCTTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCT
AAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGATGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAA
ACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATGATGATTTTGTGCTGCCTGCTACATCAAATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTG
AGAAAAAGGAGTTAATGCCTACAAATATATCTTGGATGGATTCTTGGCATTCAGATCATCAGGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAG
GACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATGAAAACAGTAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTT
CTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGATGGAGAAGTTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTA
GGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAA
GAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACC
AGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAG
ACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACA
AAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGA
TTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGG
CAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGT
TCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGA
GTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATC
CTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACA
GGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGG
GACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCTCTGGTTG
GGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGAGAGAATT
AGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAATGGTTTTTTATAGCACGCAGTCTTTGATGACTTATT
TACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTGTCCTCAGGATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCA


>TRINITY_DN131401_c4_g2_i6 from sample A1 / Example unspliced isoform:
GAAAAAATAAATTTCTATTATATATCCTCACATTCTTGCAATTCTTTCGTTTCGAATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAA
GAATGAGAGCAAGATCCATACAATGAGAGAAGACATgtacttgtgcattaatctcaaaatatttttgcttggattgcctctaattcggttttgcattgggatttttaacattagagagagttcagtccaatttttataagccaaggcgatggatgctttttgggcatttacttgctaaaatggggaaactttgaaatttt
ctgcagAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGT
TTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCACC
TGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTC
CTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTC
TTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGA
TGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATG
ATGATTTTGTGCTGCCTGCTACATCAAATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGATGGATTCTTGGCATTCAGAT
CATCAGGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATGAAAACAG
TAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGATGGAGAAG
TTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGGTTTGATGCAAAACCTGTTCACCCTAGTTTAGTTCTGAGG
TATCTTGGCAGGCATTGTGTGTCATTCATAAATTTGTTTTATTATTTTTAGAACATAAACAGTTATTTTACTAAGATCTATTTTTGATTAGATTTATGTTCAATATGGGGGTATGAGATTATTATATATGGCATGGTATGATTTAATTATCTGGGAAGATCCTTGATCTTCATGGTTTCTTTATATTTCGAAATTGTCAC
ATGTGTTACTGTGCATGTTTCAGCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTC
TGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGA
AATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAA
AGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGT
ACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTG
GGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGT
TTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGAT
GAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATAT
CTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGAT
ATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGC
AACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGG
TTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCT
CTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGA
GAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAATGGTTTTTTATAGCACGCAGTCTTTGATG
ACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTGTCCTCAGGATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCA



Intron 3 GTTAGTTTAC ... ACATGATTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A133.847824
A223.436411
A34428222
A425451015
A54813512
A642.86403
A731.581316
A850323
A941.181007
A1033.33603



>TRINITY_DN104690_c3_g1_i10 from sample A5 / Example spliced isoform
ATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAAGAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTC
AAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTGAAATTTTCTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAAT
CAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGTTTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTC
CACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCGCCTGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGT
TTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTCCTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACAT
GCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTCTTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTAC
CAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGATGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAA
ATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATGATGATTTTGTGCTGCCTGCTACATCAAATGAGGATGATAAGAATCTGCACTCTAG
TCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGATGGATTCTTGGCATTCAGATCATCAGGGTAAAAGCGTTATTAACTGTTTGGTGGTTTTGTTCATCTCAAACTTTT
GATATCCCATTTTTTCAATTCTTAAAATTGTGATGTTCTTGAATAAAACTGCAAAAATTACAGAGTGTTATTAGTACTCGATGGGCAAT


>TRINITY_DN104690_c3_g1_i8 from sample A5 / Example unspliced isoform:
ATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGgttagtttacttgcatgcttatgccatgttagggttttaacatttttcttctgccgtgcttttgcaagaatggaatccactgctctgttatgttgtattctt
attccaattgttgaagtcggctgctctcagcggatgtttttggtcaacattgccaatctcacatgattagGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAAGAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAA
TTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTGAAATTTTCTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGAT
CCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGTTTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTT
TCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCGCCTGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTC
CTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTCCTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCA
TTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTCTTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATAT
TGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGATGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATC
AGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATGATGATTTTGTGCTGCCTGCTACATCAAATGAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCT
GATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGATGGATTCTTGGCATTCAGATCATCAGGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGA
AGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATGAAAACAGTAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAG
CTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGATGGAGAAGTTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTAT
AGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTC
GTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAG
AGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGA
AAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGC
ACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAG
ATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAA
GACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCA
TCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATA
GGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTT
GGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAG
CCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAG
GCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGA
CACAAATCATTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAA
ACAAGGGCACAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGGGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGA
TAATAATGACTCGAAAACAACAGAATGGTGTTATATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTATCCTCAGGATAGATATGATGCTTAACTGTGCCCCCAGCTTCTGAGGGTGTGCTCATAAGTTCATTATTTGGT



Intron 4 GTAGATTTCC ... TTCTGGGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A187.5177
A2100043
A366.675146
A480.952134
A533.33110
A6-1000
A7100033
A8-1000
A9100010
A10100011



>TRINITY_DN119561_c2_g1_i2 from sample A3 / Example spliced isoform
GGTTAGTTTACTTGCATGCTTATGCCATGTTAGGGTTTTAACATTTTTCTTCTGCCGTGCTTTTGCAAGAATGGAATCCACTGCTCTGTTATGTTGTATTCTTATTCCAATTGTTGAAGTCGGCTGCTCTCAGCGGATGTTTTTGGTCAACATTGCCAATCTCACATGATTAGGTTTTTGAGGGTTCACTTTGTTGTTCA
GTGGTTTGGGTTTCTGTTTTCTTTGGCATTTAATTAGGGGTCACTGTTGCAGTAAGTATCGTTGAGGGCCGGTGCTCCTGCTTGCAT


>TRINITY_DN119561_c2_g1_i3 from sample A3 / Example unspliced isoform:
GGTTAGTTTACTTGCATGCTTATGCCATGTTAGGGTTTTAACATTTTTCTTCTGCCGTGCTTTTGCAAGAATGGAATCCACTGCTCTGTTATGTTGTATTCTTATTCCAATTGTTGAAGTCGGCTGCTCTCAGCGGATGTTTTTGGTCAACATTGCCAATCTCACATGATTAGGTTTTTGAGGGTTCACTTTGTTGTTCA
GTGGTTTGGGTTTCTGTTTTCTTTGGCATTTAATTAGGGGTCACTGTTGCAGTAAGTATCGTTGAGgtagatttccagagctgtttatgcatcaaatgttcaatcccaccatcatactgaaaatactttttgacagtagaagttcaccagattaatgtacacaatacatatggtattccatagtggacatgaaaatgaat
atgggattccctttaatggacatggcaatcccattgagttttggttgaacttgagatttctgtttgccatattttgaaaaggaaaaataattgcaatatatagaatatgcggttgattaagttacactgaatatgcattaatggatggttttctgggcagGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTC
GGCCCAAGAATGAGAGCAAGATCCATACAATGAGAGAAGACATAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCAT
GATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGTTTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGC
CGCCATTTGAACATCATCATTTCCCGGGGCCACCACCTGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCA
TATACATTACCTCAGCACATGCTGCCGCCACCACCTCCTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATT
GGAATTTTCTCAACCACCCCCACCTTCAACAGATGTCTTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAA
AGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGATGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTA
CCATCATCTCCTGCAGTGTCAGACATGGATATGGATGATGATTTTGTGCTGCCTGCTACATCAAATGAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCC
TACAAATATATCTTGGATGGATTCTTGGCATTCAGATCATCAGGGTAAAAGCGTTATTAACTGTTTGGTGGTTTTGTTCATCTCAAACTTTTGATATCCCATTTTTTCAATTCTTAAAATTGTGATGTTCTTGAATAAAACTGCAAAAATTACAGAGTGTTATTAGTACTCGATGGGCAAAATAAAAAAGTGTTAAACAT
GTATTCCTTGGCCAAGATCGATTCAAAGTTTGCATGAGATAAAAAATAAACTTCAAACTCCATAGTAATCTGCATAATGTTCATCAAAATTACAATAAGTCAAAAAACATGGGTTGGGCAGTGATTTTGCTTTCAGTCAATGTGACAGTAAGTTCTGGGGCCTGCCTGTCTAGCTTTTTAATCCCAAGGAGGAGTTGCCT
TTCTGGTAATAGTGTAACTTCTTTTGCCATATTGTCCCCTTCAAATTACATTGACATCTAATTGCTCTATTTTCCACAACCATGAGGTCATATTTCTCACCTGAGCTTTTCCATATAACTACTGACAGACTAGCTGTGAATCTCAATAAACTTGTAACCTTGGATGTTCCTGCTCAAGTCTATTGACATCAGATTGATAA
CTACCTTTACTTCTGCTGGATTATTGGTGCAAATCCCCATGCCGAATTGGAAGTATCCTTTGTGTTCTTCTGAAAAGCATATTGTTCCCACAGCCCCCCAAGGTCTGGATTCCCTGTGGAGTCTCTGTCAAAAAATCCTTTTATCTTCGAGTAATCAGTGGCAAGCATCTTAATCTTTTTCTGAAATATTTTTTTAAAAG
CTTTGGCATATTGGAGGGATTGTTTGAGTTTGCAGAAAACAGCAATGGGTGATGTGTTCTCTTCCCTAAACAGTTTTACATTCTTTTGAATCAAAATATACCAAATGAAGTAGAATGGTATGGATAGGATGCTTGATCTGTCTTTGTATTCTATATATCAGCCTTTGTCTCCTCCAAAGTTCCAAACCCTCCCCTTCCAT
CCTTTATTCCAATCATGGT



Intron 5 GTGAGAAAAA ... TCTTTGCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A183.4710.55848
A278.49103835
A361.9243939
A480.8273524
A584.623249
A6100092
A778.3882632
A880.653169
A956.458.5616
A1064.98172538



>TRINITY_DN131401_c4_g2_i4 from sample A1 / Example spliced isoform
ATTTATCTTACAACTCGACAATACGAGCTGCTAATGGTGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCC
CATAATCATGTAGGGCCAACATATGAAAACAGTAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAA
AGAACCTGGTCAAGAAATCTTTGATGGAGAAGTTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAG


>TRINITY_DN131401_c4_g2_i11 from sample A1 / Example unspliced isoform:
GAAAAAATAAATTTCTATTATATATCCTCACATTCTTGCAATTCTTTCGTTTCGAATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAA
GAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTGAAATTTT
CTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGT
TTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCACC
TGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTC
CTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTC
TTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGA
TGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATG
ATGATTTTGTGCTGCCTGCTACATCAAATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGgtgagaaaaaggagttaatgcctacaaatatatcttggatggattcttggcattcagat
catcaggtcccatctttgccagGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCC
AACATATGAAAACAGTAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAA
TCTTTGATGGAGAAGTTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGAGC
AGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGA
TTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCAC
ATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCT
CATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTC
CAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTG
TGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGAT
ACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTT
ATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTAC
CCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGA
ATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGA
GAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAA
TTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCC
TCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATAGC
AACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAATGGTTTTTTATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTG
TCCTCAGGATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCA



Intron 6 GTTTGATGCA ... CATGTTTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A119.391062625
A223.3791830
A321.78881732
A422.76561221
A525.2461813
A614.943749
A715.27111931
A819.051726
A922.0830611
A1013.0470714



>TRINITY_DN131401_c4_g2_i13 from sample A1 / Example spliced isoform
GAAAAAATAAATTTCTATTATATATCCTCACATTCTTGCAATTCTTTCGTTTCGAATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAA
GAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTGAAATTTT
CTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGT
TTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCACC
TGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTC
CTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTC
TTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGA
TGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATG
ATGATTTTGTGCTGCCTGCTACATCAAATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGATGGATTCTTGGCATTCAGAT
CATCAGGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATGAAAACAG
TAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGATGGAGAAG
TTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAGGTCACCT
GGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCT
TGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGA
TCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGA
GAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACA
TGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGA
AAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCA
TTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTT
ACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCC
TCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTG
CTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTAT
TAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGG
AGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGTGCGTTTGCCAAACTGAAATTTATGTCAACTTGAGGAGTTGTATTGACATCAAAATGAGTACTAATATGTCTAAACAATTTCTCCTTCAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTT
TTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTT
GTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAAT
GGTTTTTTATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTGTCCTCAGGATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCA


>TRINITY_DN131401_c4_g2_i6 from sample A1 / Example unspliced isoform:
GAAAAAATAAATTTCTATTATATATCCTCACATTCTTGCAATTCTTTCGTTTCGAATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAA
GAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTGAAATTTT
CTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGT
TTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCACC
TGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTC
CTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTC
TTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGA
TGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATG
ATGATTTTGTGCTGCCTGCTACATCAAATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGATGGATTCTTGGCATTCAGAT
CATCAGGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATGAAAACAG
TAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGATGGAGAAG
TTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGgtttgatgcaaaacctgttcaccctagtttagttctgagg
tatcttggcaggcattgtgtgtcattcataaatttgttttattatttttagaacataaacagttattttactaagatctatttttgattagatttatgttcaatatgggggtatgagattattatatatggcatggtatgatttaattatctgggaagatccttgatcttcatggtttctttatatttcgaaattgtcac
atgtgttactgtgcatgtttcagCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTC
TGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGA
AATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAA
AGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGT
ACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTG
GGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGT
TTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGAT
GAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATAT
CTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGAT
ATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGC
AACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGG
TTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCT
CTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGA
GAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAATGGTTTTTTATAGCACGCAGTCTTTGATG
ACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTGTCCTCAGGATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCA



Intron 7 GTTTTTGAGG ... TATCGTTGAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A129.4124101
A215.381120
A340.5422155
A434.781582
A553.8512141
A60300
A725620
A80300
A90700
A100300



>TRINITY_DN119561_c2_g1_i5 from sample A3 / Example spliced isoform
GGTTAGTTTACTTGCATGCTTATGCCATGTTAGGGTTTTAACATTTTTCTTCTGCCGTGCTTTTGCAAGAATGGAATCCACTGCTCTGTTATGTTGTATTCTTATTCCAATTGTTGAAGTCGGCTGCTCTCAGCGGATGTTTTTGGTCAACATTGCCAATCTCACATGATTAGGGCCGGTGCTCCTGCTTGCATGCCTTT
TGAAGAAACAGTCGGCCCAAGAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAA
TGGGGAAACTTTGAAATTTTCTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTC
CTCCAACCATGCCACCTGGTTTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCAT
CATTTCCCGGGGCCACCACCTGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCA
CATGCTGCCGCCACCACCTCCTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCAC
CCCCACCTTCAACAGATGTCTTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTT
GATGGAGCACCTAGCAGTGATGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGT
GTCAGACATGGATATGGATGATGATTTTGTGCTGCCTGCTACATCAAATGAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGA
TGGATTCTTGGCATTCAGATCATCAGGGTAAAAGCGTTATTAACTGTTTGGTGGTTTTGTTCATCTCAAACTTTTGATATCCCATTTTTTCAATTCTTAAAATTGTGATGTTCTTGAATAAAACTGCAAAAATTACAGAGTGTTATTAGTACTCGATGGGCAAAATAAAAAAGTGTTAAACATGTATTCCTTGGCCAAGA
TCGATTCAAAGTTTGCATGAGATAAAAAATAAACTTCAAACTCCATAGTAATCTGCATAATGTTCATCAAAATTACAATAAGTCAAAAAACATGGGTTGGGCAGTGATTTTGCTTTCAGTCAATGTGACAGTAAGTTCTGGGGCCTGCCTGTCTAGCTTTTTAATCCCAAGGAGGAGTTGCCTTTCTGGTAATAGTGTAA
CTTCTTTTGCCATATTGTCCCCTTCAAATTACATTGACATCTAATTGCTCTATTTTCCACAACCATGAGGTCATATTTCTCACCTGAGCTTTTCCATATAACTACTGACAGACTAGCTGTGAATCTCAATAAACTTGTAACCTTGGATGTTCCTGCTCAAGTCTATTGACATCAGATTGATAACTACCTTTACTTCTGCT
GGATTATTGGTGCAAATCCCCATGCCGAATTGGAAGTATCCTTTGTGTTCTTCTGAAAAGCATATTGTTCCCACAGCCCCCCAAGGTCTGGATTCCCTGTGGAGTCTCTGTCAAAAAATCCTTTTATCTTCGAGTAATCAGTGGCAAGCATCTTAATCTTTTTCTGAAATATTTTTTTAAAAGCTTTGGCATATTGGAGG
GATTGTTTGAGTTTGCAGAAAACAGCAATGGGTGATGTGTTCTCTTCCCTAAACAGTTTTACATTCTTTTGAATCAAAATATACCAAATGAAGTAGAATGGTATGGATAGGATGCTTGATCTGTCTTTGTATTCTATATATCAGCCTTTGTCTCCTCCAAAGTTCCAAACCCTCCCCTTCCATCCTTTATTCCAATCATG
GT


>TRINITY_DN119561_c2_g1_i2 from sample A3 / Example unspliced isoform:
GGTTAGTTTACTTGCATGCTTATGCCATGTTAGGGTTTTAACATTTTTCTTCTGCCGTGCTTTTGCAAGAATGGAATCCACTGCTCTGTTATGTTGTATTCTTATTCCAATTGTTGAAGTCGGCTGCTCTCAGCGGATGTTTTTGGTCAACATTGCCAATCTCACATGATTAGgtttttgagggttcactttgttgttca
gtggtttgggtttctgttttctttggcatttaattaggggtcactgttgcagtaagtatcgttgagGGCCGGTGCTCCTGCTTGCAT



Intron 8 GTGAGAAAAA ... TCTTTGCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A150.79314915
A247.37202511
A346.88343327
A443.5324298
A555.111234
A641.18570
A748.28151711
A851.359163
A936.3614610
A1042.86241818



>TRINITY_DN43381_c0_g1_i11 from sample A9 / Example spliced isoform
CTCACATTCTTGCAATTCTTTCGTTTCGAATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAAGAATGAGAGCAAGATCCATACAATGA
GAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTGAAATTTTCTGCAGAAACTTCAATTTATTGGCAC
TTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGTTTTCCAGGTGGACAACAACAGAATTT
CGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCACCTGGACCGCCATTACCTGGTCCACCTC
CTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTCCTGCAGTGGGGATTTCTCAATCAAGT
CAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTCTTGGACCCACCTCCTTCTTCACATTC
AACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGATGCTGCCATTGGATATCAGTATTTTC
AGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATGATGATTTTGTGCTGCCTGCTACATCA
AATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGA
AGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATGAAAACAGTAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATA
GGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGATGGAGAAGTTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAAT
GAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAA
GTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACA
GCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGAC
TTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTT
TCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTA
CTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCAT
TTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAG
TTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGAT
CACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGT
GGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCT
CGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGA
GCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCA
TTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCA
CAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGA
CTCGAAAACAACAGAATGGTGTTATATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTGTCCTCAGGATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCATAAGTTCATTATTTGGTTCCTTTAACATTTGCAACCTTTTTATTGTGGTTGA
TGCTATTTCTGACTTTCCATATGTCCTATCTGGTTTGATTAATTTTGTACATGATGTGTTCTATCACTATGAAATCTTGGATGTACTGTCAATGTTTCTGCTCTTGATGTTCTAGCTGACATCAACATGATTATCTTTGTTTTTCAGGCTTATCTTGAAAGTTGTTTTGACTTTTGATAACTTCTATATTTTGTTACTTT
TAGTAAGTAAGAAATTTTTTGTCCACACATATTCTTCATCTGAACTGTTTGAACATATAAGGGTATTGAGCTGGTTTCACTGTCTCTGAAATCTTGGCCTACTTTGGTTACATACAATGGCAAATATAAA


>TRINITY_DN43381_c0_g1_i9 from sample A9 / Example unspliced isoform:
CTCACATTCTTGCAATTCTTTCGTTTCGAATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAAGAATGAGAGCAAGATCCATACAATGA
GAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTGAAATTTTCTGCAGAAACTTCAATTTATTGGCAC
TTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGTTTTCCAGGTGGACAACAACAGAATTT
CGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCACCTGGACCGCCATTACCTGGTCCACCTC
CTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTCCTGCAGTGGGGATTTCTCAATCAAGT
CAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTCTTGGACCCACCTCCTTCTTCACATTC
AACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGATGCTGCCATTGGATATCAGTATTTTC
AGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATGATGATTTTGTGCTGCCTGCTACATCA
AATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGgtgagaaaaaggagttaatgcctacaaatatatcttggatggattcttggcattcagatcatcaggtgaccatgtctggataatc
tgagcccagtcccatctttgccagGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGG
CCAACATATGAAAACAGTAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGA
AATCTTTGATGGAGAAGTTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGA
GCAGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAA
GATTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGC
ACATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTG
CTCATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTT
TCCAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCG
TGTGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAG
ATACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCT
TTATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGT
ACCCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTA
GAATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACAT
GAGAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAG
AATTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATG
CCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATA
GCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAATGGTGTTATATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACC
TGTCCTCAGGATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCATAAGTTCATTATTTGGTTCCTTTAACATTTGCAACCTTTTTATTGTGGTTGATGCTATTTCTGACTTTCCATATGTCCTATCTGGTTTGATTAATTTTGTACATGATGTGTTCTATCACTATGAAATCTTGGATGTACTGTCA
ATGTTTCTGCTCTTGATGTTCTAGCTGACATCAACATGATTATCTTTGTTTTTCAGGCTTATCTTGAAAGTTGTTTTGACTTTTGATAACTTCTATATTTTGTTACTTTTAGTAAGTAAGAAATTTTTTGTCCACACATATTCTTCATCTGAACTGTTTGAACATATAAGGGTATTGAGCTGGTTTCACTGTCTCTGAAA
TCTTGGCCTACTTTGGTTACATACAATGGCAAATATAAA



Intron 9 GTTTTTGAGG ... TTCTGGGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A136241017
A238.8911311
A342.86221914
A44015911
A54012142
A625302
A740626
A814.29301
A922.22704
A1014.29301



>TRINITY_DN100814_c1_g1_i8 from sample A4 / Example spliced isoform
GGTTAGTTTACTTGCATGCTTATGCCATGTTAGGGTTTTAACATTTTTCTTCTGCCGTGCTTTTGCAAGAATGGAATCCACTGCTCTGTTATGTTGTATTCTTATTCCAATTGTTGAAGTCGGCTGCTCTCAGCGGATGTTTTTGGTCAACATTGCCAATCTCACATGATTAGGGCCGGTGCTCCTGCTTGCATGCCTTT
TGAAGAAACAGTCGGCCCAAGAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAA
TGGGGAAACTTTGAAATTTTCTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTC
CTCCAACCATGCCACCTGGTTTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCAT
CATTTCCCGGGGCCACCGCCTGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCA
CATGCTGCCGCCACCACCTCCTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCAC
CCCCACCTTCAACAGATGTCTTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTT
GATGGAGCACCTAGCAGTGATGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGT
GTCAGACATGGATATGGATGATGATTTTGTGCTGCCTGCTACATCAAATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGA
TGGATTCTTGGCATTCAGATCATCAGGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTA
GGGCCAACATATGAAAACAGTAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCA
AGAAATCTTTGATGGAGAAGTTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCG
GGAGCAGAAACAGGTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAA
CAAGATTTATTGGGGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTC
TGCACATTTAGATAGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTC
GTGCTCATCTTTTGCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCC
TTTTCCAGAGAAAATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCG
TCGTGTGAATCTGCTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAG
AAGATACACGCTTTGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCC
TCTTTATTAAGCAAGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATC
TGTACCCCTTCAATCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTG
GTAGAATCAAAACTGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCA
CATGAGAAAAGCAGAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGA
GAGAATTGAGCAGTATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCA
ATGCCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGA
ATAGCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAATGGTTTTTTATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAA
ACCTGTCCTCAGGATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCATAAGTTCATTATTTGGTTCCTTTAACATTTGCAACCTTTTTATTGTGGTTGATGCTATTTCTGACTTTCCATATGTCCTATCTGGTTTGATTAATTTTGTACATGATGTGTTCTATCA


>TRINITY_DN100814_c1_g1_i2 from sample A4 / Example unspliced isoform:
GGTTAGTTTACTTGCATGCTTATGCCATGTTAGGGTTTTAACATTTTTCTTCTGCCGTGCTTTTGCAAGAATGGAATCCACTGCTCTGTTATGTTGTATTCTTATTCCAATTGTTGAAGTCGGCTGCTCTCAGCGGATGTTTTTGGTCAACATTGCCAATCTCACATGATTAGgtttttgagggttcactttgttgttca
gtggtttgggtttctgttttctttggcatttaattaggggtcactgttgcagtaagtatcgttgaggtagatttccagagctgtttatgcatcaaatgttcaatcccaccatcatactgaaaatactttttgacagtagaagttcaccagattaatgtacacaatacatatggtattccatagtggacatgaaaatgaat
atgggattccctttaatggacatggcaatcccattgagttttggttgaacttgagatttctgtttgccatattttgaaaaggaaaaataattgcaatatatagaatatgcggttgattaagttacactgaatatgcattaatggatggttttctgggcagGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTC
GGCCCAAGAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTG
AAATTTTCTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCC
ACCTGGTTTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGC
CACCGCCTGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCA
CCACCTCCTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAAC
AGATGTCTTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTA
GCAGTGATGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGAT
ATGGATGATGATTTTGTGCTGCCTGCTACATCAAATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGATGGATTCTTGGCA
TTCAGATCATCAGGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATG
AAAACAGTAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGAT
GGAGAAGTTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAG
GTCACCTGGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGG
GGTTTCTTGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGAT
AGAGAGATCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTT
GCCAAGAGAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAA
ATCTACATGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTG
CTGAGGAAAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTT
TGAGTCATTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCA
AGCCTTTACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAA
TCAACCCTCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAAC
TGGTTTGCTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCA
GAAGTATTAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAG
TATCTGGAGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTT
TTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCA
GTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAATGGTTTTTTATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTGTCCTCAGG
ATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCATAAGTTCATTATTTGGTTCCTTTAACATTTGCAACCTTTTTATTGTGGTTGATGCTATTTCTGACTTTCCATATGTCCTATCTGGTTTGATTAATTTTGTACATGATGTGTTCTATCA



Intron 10 GTGCGTTTGC ... TCTCCTTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A123.91214333
A228.81633417
A327.271085130
A430.94482419
A522.0830134
A624.642087165
A724.342027456
A820.82579243
A922.142186856
A1019.41625424



>TRINITY_DN131401_c4_g2_i9 from sample A1 / Example spliced isoform
GAAAAAATAAATTTCTATTATATATCCTCACATTCTTGCAATTCTTTCGTTTCGAATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAA
GAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTGAAATTTT
CTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGT
TTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCACC
TGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTC
CTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTC
TTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGA
TGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATG
ATGATTTTGTGCTGCCTGCTACATCAAATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGATGGATTCTTGGCATTCAGAT
CATCAGGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATGAAAACAG
TAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGATGGAGAAG
TTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAGGTCACCT
GGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCT
TGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGA
TCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGA
GAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACA
TGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGA
AAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCA
TTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTT
ACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCC
TCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTG
CTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTAT
TAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGG
AGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTTTTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTTTTGATGT
CAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTTGTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCAGTTTTGC
CTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAATGGTTTTTTATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTGTCCTCAGGATAGATA
TGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCA


>TRINITY_DN131401_c4_g2_i13 from sample A1 / Example unspliced isoform:
GAAAAAATAAATTTCTATTATATATCCTCACATTCTTGCAATTCTTTCGTTTCGAATTTTATCTACTCCAAATTTTCTTCATTTCGGCTCTCGATTGAGAGCGTTGTTGTGCTTATCCGAGGCTCTCGGACGAATCACTCTTTTACGCCGAAGGGCCGGTGCTCCTGCTTGCATGCCTTTTGAAGAAACAGTCGGCCCAA
GAATGAGAGCAAGATCCATACAATGAGAGAAGACATGTACTTGTGCATTAATCTCAAAATATTTTTGCTTGGATTGCCTCTAATTCGGTTTTGCATTGGGATTTTTAACATTAGAGAGAGTTCAGTCCAATTTTTATAAGCCAAGGCGATGGATGCTTTTTGGGCATTTACTTGCTAAAATGGGGAAACTTTGAAATTTT
CTGCAGAAACTTCAATTTATTGGCACTTCTGTTGTATTGTGAAAGAAAGTAAAATCAGAATGTATAGGCCAGGAAGATATGATCCTCAATATGGGCATGGTGCTCCAGGGGTTCCCATTCAACAGTACCCTGGGCCTGAGCCTAGACCTTTCTACCCTCCCATGATGCAGGGAGGAGGTCCTCCAACCATGCCACCTGGT
TTTCCAGGTGGACAACAACAGAATTTCGGCTACGGGCCTCCTCCCGCAATACCTCCACAGGTATCTCCTATGTCACCTGGGTTTCCTCCAGTTTCAATGCAATCTGTACCGAATCATGGAAATCAATCCTCTGCACCTCCATTTGGAGTGTTTCCCTCTAGGCCGCCATTTGAACATCATCATTTCCCGGGGCCACCACC
TGGACCGCCATTACCTGGTCCACCTCCTCAGCTTCCTATACCTCGTGCTGTCAGTTTTATTGGTACACTTCCTCATGGTCCTCCTCCACCCCAACCTCCTTCATTTTCTCCATTATTGCCACCACATTCTTCAGCATTTGAGTTTTCTCAACCAGCTCCGCCATATACATTACCTCAGCACATGCTGCCGCCACCACCTC
CTGCAGTGGGGATTTCTCAATCAAGTCAATTTTCACAAACAGTACCTCAGAACATGCCACAACCTCCACTACTGTCTTCTGCATTCGATGCTTGTGAACTACCTCCACCTCCACACCCACAGCCACTCTATCCGCCACAGTATTTGCCGCAACCTCCTGCATTGGAATTTTCTCAACCACCCCCACCTTCAACAGATGTC
TTGGACCCACCTCCTTCTTCACATTCAACATCTTTACCTCCACCTCCACAGTTACCAAACGATGAAGAAATGGTTCAGAATATTGAAATTCTTTCTGAATTTGTTGTTAAGAATGGGCCTGAGTTTGAAAATATGGCCCGGGCTAAGCAAGCAGGTAATCCAAAGTTCTCATTTTTGTTTGATGGAGCACCTAGCAGTGA
TGCTGCCATTGGATATCAGTATTTTCAGTGGATGAAAAAAAAGTGGGAACTGCAAATAACTACAGGTCAGCAGGTGGAAAATCAGGGTCAGATTATAGAACCATTGGAGATAACCATGCATAGCCAACAATCTATAGATCCAAAACTTGCCGTGGAACAAGTACCATCATCTCCTGCAGTGTCAGACATGGATATGGATG
ATGATTTTGTGCTGCCTGCTACATCAAATAAGGATGATAAGAATCTGCACTCTAGTCATGCAACAGTACTATGCAAAACTTCTGATGAACAAGAAAGTGGGAATTTATCTTACAACTCGACAATAAGAGCTGCTAATGGTGGTGAGAAAAAGGAGTTAATGCCTACAAATATATCTTGGATGGATTCTTGGCATTCAGAT
CATCAGGGAGAACTTCGACAAACGACAAAGTTTGACGACCAACCTGTTGCCAATGAGAGTAACAGGAATTCTTCCTTCAATGAAGTGCCAGAAGCTGGATCTAATGCAATACCAGCAGAGAATGATCCACTCAGGAATAAACAGGACTCGAGGGTTGCGAATCTTCCCCATAATCATGTAGGGCCAACATATGAAAACAG
TAAAACACTGCAGACCAGTGGATTATCTCATAAGACTGAGGAGAACATGGCAGATGATGATATTGGATACAAGGAAATGCAAGCTCCCAATAGGAATGTATTGAGAGAGACTAATAACTATTCAAAGGATTGGGGTCAGCCTTTCTCATGGAATATTGATGTTACAAAAGAACCTGGTCAAGAAATCTTTGATGGAGAAG
TTCCTGCAAAGCAAGACGAGGAAATGGTTGAAAACTATGTAAAAGAGGGGGCAAGTGACAGTGAATCAGATGAGGTGCTGTATAGTGCAAATGAATTAAGAAAAGGTCGCAGAAGCAGCAAAAGCCGGTCACCAGAAGGCTCTAGGTTGAGATCTCGCAGCCGCTCTCCGAGGCAAGGCGGGAGCAGAAACAGGTCACCT
GGGATAGGAGATGACAAGAATAGTGATGAAAAAATCAATGCAATTTGTTTTTATTTTGCCAGAGGTGTGTGCGAGAAAGGGTCGTCCTGTAAGTTTCTTCATCAAAAGGCCACTGTTAAAAGTATTGTAGATTATTCTGATGAAGAAAGTGATAAGAGCAAACACACAAAAGAGGAGGAACAAGATTTATTGGGGTTTCT
TGGCGGTTTTGAACGCTCTGCTATAGTGGATGGCAAGCTCAAGAGTACTTCTCCTGGCGGGCATGAGAACCCTGGTGAAAGAGAGGAAAACAGCCAGGGGGAAACTAAAAGTGATGATTCTTCTGGGAAAGACCAGAAATCACCAGAATGGGATGTTGATATCTTTTCAGAGTCATATTCTGCACATTTAGATAGAGAGA
TCTTTCGACATTCTTTGCAGCAGAGAGAAAAGTCGGGGGCACAGCGCTTGCCAAGGGATGACTTTCGGCCACTTCCATTGCGAAAGGAAGACTTTGAACCACAGGCCCCCTCACGAGAAGACACAGGCTCCTTGCAAAGAGAAGACTCACGAGCACAATTTTTACGTGGAGATGATTCTCGTGCTCATCTTTTGCCAAGA
GAAGAGGTGCACCCTTTATCTTTGGCTCAGGAAGATATGCAAAGGTTGCGTTCACAACCTTTACACCGGGACGATGTACGGGCACACGCTTTTCTCCGAGAAGATTTACAGACACAGTATTTAATTCGTGAAGATGTACGTACAAAAGGTTTGCTTCGGGAAGGTGTATCTAGAGAACCCTTTTCCAGAGAAAATCTACA
TGCAGCTTCTTTGCTTCGAGAAGATTTACGTCCACATTCTTTACATCGTGAAGATTTACGTGCACATCCTTTACTTCGTGAAGATTTACGTACTCAGCCTATGTTCAGAGGAGGTGCCTATGGTCAGTCCTTGATTGGGGATGATTTACGACTGCCATCTTCTCTTAGGGATGATTACCGTCGTGTGAATCTGCTGAGGA
AAGATATTCGTTTAGAGCCACTGCTAAGAGGTGATTCACGATTTGAGGATGAAGACTTGCGATCTCATTCAATTCCCCGTGAAGACTTGCATTTTCAGCATTTACTGAGACAAAATTCTCGAGTTCGTAGTGAAAGTTTATTGGCAAGAGATGATTTTCGTCCAAATCTCTTTTCAAGAGAAGATACACGCTTTGAGTCA
TTGGCGAATGATTTGCCTGCTCATCGGCTAAATGTTCCAATGGGAAGATTTGAATCACTTCTATCTAGAAGTCCTTCTATTCATCATAACAGTTCCTTGCCTATGAATTCTTCTTTATCATCGCATGTGAATTTGATGAGTAGTTCTGATCCTCTTCGTCTGGGGCACTTAACACGTTCCTCTTTATTAAGCAAGCCTTT
ACAGCATTCCGAAATGGCAGAACGTCTATTTGCAATGCGAGAGCAAGATCGTAGGTCACCTCCTTCGCCACCAACGGTTTATAGGACTGGATCACAACTCCAGTCAACACCCTCTGGTTCAATGCCACTTGGTATATCTGAAGAGTTGCAGGCATACCGCTATTCACATGCAGGATTATCTGTACCCCTTCAATCAACCC
TCCGTGTATCTTCGTCTACTCCAGGAAGCCAATACGATCCTTTGTATGACAGTATTGAGCTGGGTTTTGGATCTAAGTTATTTGGCTCTGGTGGAGGGGAACATGGTCATGTTACGGGAGCTATGACAGAGTCGGATATTGATCCTCGATTGCAGAATGTTGGTCCTGAATATGATGGTGGTAGAATCAAAACTGGTTTG
CTTCGTCAGCCTGATCGTAAGTTTGAAGCTGATGGACTTGAAGCAGCTACAGATGCTGAAGTAGGTGTAGTTGATAATGAAAGCCCCCGCCTCGAAGGAGCGAAGGACTGGAGCCCTGGTCATCCATTGGAAGTAGCAACTACAGGTGCTGGAGAGATTGATGTGGATCAGACGCATGCACATGAGAAAAGCAGAAGTAT
TAAGGAAATCAAGGCATTGAAAATTTTTAGAACAGCATTGGCAGAGTTTGCAAAAGAGATACTGAAGCCAACATGGCGGGAAGGCCACATGAGCAAGGAGGAATTCAAAACAATTGTTAAAAAGGCTGTTGATAAGGTTTCAGGGACATTACAAAGTCATCAAATACCAAATACTCCAGAGAGAATTGAGCAGTATCTGG
AGTCATCCCGTGTTAAGTTTACAAAGCTTGTTGAGgtgcgtttgccaaactgaaatttatgtcaacttgaggagttgtattgacatcaaaatgagtactaatatgtctaaacaatttctccttcagGGGTATGTTGAAAAGTATGTCAAAGGTTGAATTACCTGTATCAGCAGACACAAATCATTTATTCCCTGGTGTTT
TTATATACAACTACATGCCCTGCACTCTCTGGTTGGGTTTGTTGAAGCCTTTATGATTGGGAGGTAAGTCAATGCCTCATTCTTTTGATGTCAATAGACATTTGGTCTTATAGTGGTAAGGTGCATGGGCAAGTTTGTCATAAACAAATTTTATGGGGTTCTTTTCTGAGGAAAACAAGGGCACAATTGTACAGAACTTT
GTAAGACATGATGCCCTAAATATCTTGAGAGAATTAGGAAAGAGGTGAGTTAAATATATGTCATCTTTTGAATAGCAACTTTCAGTTTTGCCTTGAAAATTGCATAATAGACTCAGTAAAGCCTCTGATTTGAGTAGGGTCTTATCCTTGAAGCTATTTGGAATGGATTCATGATAATAATGACTCGAAAACAACAGAAT
GGTTTTTTATAGCACGCAGTCTTTGATGACTTATTTACGTCATATGAATTTTCATTTGAGATCTTATGCAAACCTGTCCTCAGGATAGATATGATGCTTAACTGTGCTCCCAGCTTCTGAGGGTGTGCTCA