NA ----> NA

Intron 1 GCGCTGGAGC ... TGTTTTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	95.26	17	13	342
A2	96.3	16	15	416
A3	93.57	31	10	451
A4	95.47	13	10	274
A5	91.67	13	7	143
A6	97.86	10	21	458
A7	93.94	82	77	1272
A8	93.7	86	95	1278
A9	94.71	46	41	824
A10	96.1	47	56	1157

>TRINITY_DN43339_c1_g2_i5 from sample A9 / Example spliced isoform

AATTTATGTGTTTGTGTTATTGTTGTTTGTGTTGTTGTTTGTTTGTGTTGTTTGCAAATAATTTTATCATAGAACTGAATGTTGGTCTTAAGATCAATCTTTGTGAAGGTTCCTTTTTATGTATTTTTTGAGCTGTCTTCAGTGGCTACTTACACTATGTTTCTGAGACAGTTATGATCCAGGTTTGCTGGGTCTTTTGA
AGACGAGGACTTTATTGTACCATTTGATTTAATAAGAATAACAATGGTATTGGAAATGGACGTCTTATTCTTTGGCGAAGGAGAGAGGAGGCCCGGACGCCCGGGAGTTTGTACAGGAGAGGCGGGGAGTTCGTACATGAGAGGAGCGGAGCCGGGAGCCTGGAGTTCGTACATGAGAGGAGCGGAGCCGGGGAGCCTGG
AGCCCGGAGTTTGTACATGAGAGGCGCTGGAGCCTGGAGCCTGGAGTTCGTACATGAGAGGCGCTGGAGCCGGGAGCCTGGAGCATGGAGTTAGTACATGAGAGGAGCGGAGCCGGGAGCCTGGACTTTGTACATGAGAGGCGCTGGAGCGTGGAGCCTGGAGTTCGTACATGAGATACGCTGGAGCCGGGAGCCTGGAG
CCTGGAGTTTGTACAGGAGAGGCGCTGGAGCCAGGAGCGGAGCCGGGACCACTCCCAAATTTATGAGTTCTTTGAATTTGGGAGTCTTATTGTTTGCCGCCTGGAGTTCGTACATGAGAGGAGCGGAGCCGGGGAGCCTGGAGCCCGGAGTTTGTACATGAGAGGCGCTGGAGCCTGGAGCCTGGAGTTCGTACATGAGA
GGAGCGGTTGTTGGAAGAGTAAAAGAAAGAGGAG

>TRINITY_DN43339_c1_g2_i3 from sample A9 / Example unspliced isoform:

AGGAGCGGAGCCGGGGAGCCTGGAGCCCGGAGTTTGTACATGAGAGGCGCTGGAGCCTGGAGCCTGGAGTTCGTACATGAGAGgcgctggagccgggagcctggagcatggagttagtacatgagaggagcggagccgggagcctggactttgtacatgagaggcgctggagcgtggagcctggagttcgtacatgagat
acgctggagccgggagcctggagcctggagtttgtacaggagaggcgctggagccggtgagttcgaacagaggaggaacggttctggaggaggagccgggagttagtacaggagaggagcggagccgaacatgatttaggagttctttgaatctggaagtcttattgttttcaagGAGCGGAGCCGGGAGTTGTTGGAAG
AGTAAAAGAAAGAGGAGCAGCCACGAGTTCGTATAGGAGAGGAGCGGAGCCGAACATAATTTAGGAGTTCTTTGAATCTGGAAGTCTTATTGTTTTCAGCTGTTGGAAGAGTAAAAGAAAGAGGAGCAGCCACGAGTTCGTATAGGAGAGGAGCGGAGCCGAACATAATTTAGGAGTTCTCTGAATTTGGGAGTCTTTTT
GAGTCTTTTGCCT

Intron 2 ATGAGAGGCG ... GAGTTAGTAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.48	19	10	23
A2	52.78	17	14	24
A3	26.19	31	9	13
A4	51.72	14	10	20
A5	41.67	14	7	13
A6	75	10	21	39
A7	55.45	90	93	131
A8	57.7	81	99	122
A9	55.56	46	42	73
A10	58.54	51	57	87

>TRINITY_DN43339_c1_g2_i5 from sample A9 / Example spliced isoform

AATTTATGTGTTTGTGTTATTGTTGTTTGTGTTGTTGTTTGTTTGTGTTGTTTGCAAATAATTTTATCATAGAACTGAATGTTGGTCTTAAGATCAATCTTTGTGAAGGTTCCTTTTTATGTATTTTTTGAGCTGTCTTCAGTGGCTACTTACACTATGTTTCTGAGACAGTTATGATCCAGGTTTGCTGGGTCTTTTGA
AGACGAGGACTTTATTGTACCATTTGATTTAATAAGAATAACAATGGTATTGGAAATGGACGTCTTATTCTTTGGCGAAGGAGAGAGGAGGCCCGGACGCCCGGGAGTTTGTACAGGAGAGGCGGGGAGTTCGTACATGAGAGGAGCGGAGCCGGGAGCCTGGAGTTCGTACATGAGAGGAGCGGAGCCGGGGAGCCTGG
AGCCCGGAGTTTGTACATGAGAGGCGCTGGAGCCTGGAGCCTGGAGTTCGTACATGAGAGGCGCTGGAGCCGGGAGCCTGGAGCATGGAGTTAGTACATGAGAGGAGCGGAGCCGGGAGCCTGGACTTTGTACATGAGAGGCGCTGGAGCGTGGAGCCTGGAGTTCGTACATGAGATACGCTGGAGCCGGGAGCCTGGAG
CCTGGAGTTTGTACAGGAGAGGCGCTGGAGCCAGGAGCGGAGCCGGGACCACTCCCAAATTTATGAGTTCTTTGAATTTGGGAGTCTTATTGTTTGCCGCCTGGAGTTCGTACATGAGAGGAGCGGAGCCGGGGAGCCTGGAGCCCGGAGTTTGTACATGAGAGGCGCTGGAGCCTGGAGCCTGGAGTTCGTACATGAGA
GGAGCGGTTGTTGGAAGAGTAAAAGAAAGAGGAG

>TRINITY_DN43339_c1_g2_i6 from sample A9 / Example unspliced isoform:

AGGAGCGGAGCCGGGGAGCCTGGAGCCCGGAGTTTGTACATGAGAGGCGCTGGAGCCTGGAGCCTGGAGTTCGTACatgagaggcgctggagccgggagcctggagcatggagttagtacATGAGAGGAGCGGAGCCGGGAGCCTGGACTTTGTACATGAGAGGCGCTGGAGCGTGGAGCCTGGAGTTCGTACATGAGAT
ACGCTGGAGCCGGGAGCCTGGAGCCTGGAGTTTGTACAGGAGAGGCGCTGGAGCCTGGAGTTCGTACATGAGAGGAGCGGAGCCGGGACCACTCCCAAATTTATGAGTTCTTTGAATTTGGGAGTCTTATTGTTTGCCGTTGTTGGAAGAGTAAAAGAAAGAGGAGGAGCGGAGGAGAGGAACAGAGCCGAATATAATTT
AGGAGTTCTTTGAATTTGGGTGTCTTTTTGAGTCTTTTGCCTTTTTTATTTTTATATTTTTAAATAAGCATGGCCATGCTTTTACTTATTTTTACTTTTCAATTTGTTTGCGTTTTCTTGTGTTTTCGTTTGTTTGTTTGTGTTTTCGTTTGAATGCATTCTTTTATGTTTTGTGTTTCGGAGTCTGGATTTAGATTGTT
AGGCGGAGAAAAAAAAAAAAGCTACTACTGAAAAC

Intron 3 GTGAATTTCA ... GGAGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.44	66	87	84
A2	50.2	61	69	54
A3	54.7	53	61	67
A4	53.85	45	57	48
A5	50.9	41	41	44
A6	59.16	97	144	137
A7	53.13	243	273	278
A8	52.43	348	409	358
A9	51.71	240	281	233
A10	54.48	178	217	209

>TRINITY_DN43918_c4_g2_i6 from sample A6 / Example spliced isoform

GCAGAATCAAGTCGACTATTCAGCAATATTTTAATTTGGGAAGAACATCAAGATTATGTTGTTGATATGGGATCTCTGAGTAATCCTGGTTTTGGGTTTCCTTTTTGTCTAGATGTAGATATCTTTTTGCATGTCATAGAACTGAATGTTTGTCTTAAGATCAATCTTTGTGAAGGTTCCTTTTTATGTATTTTCTGAGC
TGTTTTCAGTGGCTACTGCTGCTATGTTTCTGAGACAGTTATGATCCAGGTTTGCTGGGTCTTTTGAAGACGAGGACTTTATTGTACCATTTGATTTAATAAGAATAACAATGGTATTGGAAATGGACGTCTTATTCTTTGGCGAAGGAGAGAGGAGGCCCGGACGCCCGGGAGTTTGTACAGGAGAGGCGGGGAGTTCG
TACATGAGAGGAGCGGAGCCGGGAGCCTGGAGTTCGTACATGAGAGGAGCGGAGCCGGGGAGCCTGGAGCCCGGAGTTTGTACATGAGAGGCGCTGGAGCCTGGAGCCTGGAGTTCGTACATGAGAGGCGCTGGAGCCGGGAGCCTGGAGCATGGAGTTAGTACATGAGAGGAGCGGAGCCGGGAGCCTGGAGTTTGTAC
ATGAGAGGCGCTGGAGCGTGGAGCCTGGAGTTCGTACATGAGATCCGCTGGAGCCGGGAGCCTGGAGCCTGGAGTTTGTACAGGAGAGGCGCTGGAGCCTGGAGTTCGTACATGAGAGGAGCGGAGCCGGGACCACTCCCAAATTTATGAGTTCTTTGAATTTGGGAGTCTTATTGTTTGCCGTTGTTGGAAGAGTAAAA
GAAAGAGGAGGAGCGGAGGAGAGGAACAGAGCCGAATATAATTTAGGAGTTCTTTGAATTTGGGTGTCTTTTTGAGTCTTTTGCCTTTTTGCCTGGAGTTCGTACATGAGTATTTTTATTTTTTTTTAAATAAGCATGGCCATGCTTTTACTTATTTTTACTTTTCAATTTGTTTGCGTTTTCTTGTGTTTTCGTTTGTT
TGTTTGTGTTTTCGTTTGAATGCATTCTTTTATGTTTTGTGTTTCGGAGTCTGGATTTAGATTGTTAGGCGGAGAAAAAAAAAAAAGCTACTACCGAAAACAGCATATGAGAGCTTATAAGACAAAGACGTATGTATAGAGAAAAAAAAAAGCTATTACCGAAAACAACAAATGAGATAAGGCAAAGACGTATATGTAGA
AGAATTTCAACTACCGCAGAATTTCCAGATTGCAGGGAGATGAATAGAGGATTTTTTGACCTATGCAAACATGGTTATGTCATAGGGGATTTCTTAGTTGTGTTGAATTGCATGGTAAATGAAGACTTAGAAAGGGTAAATGAGTGGAAGATTTTTTTGACCAAAAGTAAGATGCAAGGGAAGATGAAAAATATGCTTTA
ACAAACAGCATTATGGTAAATGTAGACTGAGAAAGGGTAAATGAGTGGAAGA

>TRINITY_DN43918_c4_g2_i8 from sample A6 / Example unspliced isoform:

GCAGAATCAAGTCGACTATTCAGCAATATTTTAATTTGGGAAGAACATCAAGATTATGTTGTTGATATGGGATCTCTGAGTAATCCTGGTTTTGGGTTTCCTTTTTGTCTAGATGTAGATATCTTTTTGCATGTCATAGAACTGAATGTTTGTCTTAAGATCAATCTTTGTGAAGGTTCCTTTTTATGTATTTTCTGAGC
TGTTTTCAGTGGCTACTGCTGCTATGTTTCTGAGACAGTTATGATCCAGGTTTGCTGGGTCTTTTGAAGACGAGGACTTTATTGTACCATTTGATTTAATAAGAATAACAATGGTATTGGAAATGGACGTCTTATTCTTTGGCGAAGGAGAGAGGAGGCCCGGACGCCCGGGAGTTTGTACAGGAGAGGCGGGGAGTTCG
TACATGAGAGGAGCGGAGCCGGGAGCCTGGAGTTCGTACATGAGAGGAGCGGAGCCGGGGAGCCTGGAGCCCGGAGTTTGTACATGAGAGGCGCTGGAGCCTGGAGCCTGGAGTTCGTACATGAGAGGCGCTGGAGCCGGGAGCCTGGAGCATGGAGTTAGTACATGAGAGGAGCGGAGCCGGGAGCCTGGAGTTTGTAC
ATGAGAGGCGCTGGAGCGTGGAGCCTGGAGTTCGTACATGAGATCCGCTGGAGCCGGGAGCCTGGAGCCTGGAGTTTGTACAGGAGAGGCGCTGGAGCCTGGAGTTCGTACATGAGAGGAGCGGAGCCGGGACCACTCCCAAATTTATGAGTTCTTTGAATTTGGGAGTCTTATTGTTTGCCGTTGTTGGAAGAGTAAAA
GAAAGAGGAGGAGCGGAGGAGAGGAACAGAGCCGAATATAATTTAGGAGTTCTTTGAATTTGGGTGTCTTTTTGAGTCTTTTGCCTTTTTTATTTTTATTTTTTTTTAAATAAGCATGGCCATGCTTTTACTTATTTTTACTTTTCAATTTGTTTGCGTTTTCTTGTGTTTTCGTTTGTTTGTTTGTGTTTTCGTTTGAA
TGCATTCTTTTATGTTTTGTGTTTCGGAGTCTGGATTTAGATTGTTAGGCGGAGAAAAAAAAAAAAGCTACTACCGAAAACAGCATATGAGAGCTTATAAGACAAAGACGTATGTATAGAGAAAAAAAAAGCTATTACCGAAAACAACAAATGAGATAAGACGTATATGTAGAAGAATTTCAACTACCGCAGAATTTCCA
GATTGCAGgtgaatttcagccactgcggagttgcagGGAGATGAATAGAGGATTTTTTGACCTATGCAAACATGGTTATGTCATAGGGGATTTCTTAGTTGTGTTGAATTGCATGGTAAATGAAGACTTAGAAAGGGTAAATGAGTGGAAGATTTTTTTGACCAAAAGTAAGATGCAAGGGAAGATGAAAAATATGCTTT
AACAAACAGCATTATGGTAAATGTAGACTGAGAAAGGGTAAATGAGTGGAAGA