sp|Q5ZC88|CUL1_ORYSJ ----> Cullin-1

Intron 1 GTACGCAAAA ... TTCTTCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.91	160.5	37	81
A2	29.72	145.5	32.5	65.5
A3	21.36	155	27.5	46.5
A4	34.49	78.5	17	44.5
A5	35.69	39	9	22.5
A6	40.64	52.5	11.5	38.5
A7	28.64	205.5	37.5	92.5
A8	29.32	168	22.5	80
A9	18.39	191	16	49
A10	29.97	181.5	30	88.5

>TRINITY_DN45219_c0_g5_i2 from sample A6 / Example spliced isoform

CTTCTTGCATATATTGGTGATAAAGATCTATTTTCAGAATTTTATAGGAAAAAACTAGCACGTCGGCTTATATTTGATAAAAGTGCAAATGATGAACATGAACGGAGTATCTTAACGAAATTGAAGCAACAATGTGGAGGCCAGTTTACTTCAAAGATGGAGGGAATGCTTACTGATTTGACTTTGGCACGGGAGAACGA
ACCAAAATTTCAAAAATATCTTGATGATAATGTGCATGCACATCCTGGAATAGAATTGACTGTAACAGTCTTGACTACTGGATTTTGGCCCAGCTACAAGTCATTTGATCTCAATCTGCCTGCTGAGATGGTAAAATGTGTAGAGGTATTTAAAGAATTCTACCAGACACAAACTAAACATCGAAAGCTTACATGGATAT
ACTCTTTAGGGACATGCAATATTTTTGGCAATTTTAATGCCAGGTCGGTGGAACTTATTGTGACTACATATCAGGGATCTGTATTATTGCTGTTCAATTCTGCAGAAAAATTGAGCTACTCAGAAATCAAATCTCAGTTGAATTTGTCTGATGAAGAGGTTGTTAGATTGCTACATTCACTTTCGTGTTTAAAACACAAA
GTCTTGAATAAGGAGCCAAACACAAAAACTGTCACACAATCTGATTACTTCGAATTTAATGCAAAATTTACTGACAAATCAAGAAAGATTAAGATTCCTCTTTCTCCTGTGGATGAGAAGAAGACAATAATTGAGGACGTGGACAAAGATAGACGATATGCAATTGATGCTTGTATTGTTCGAATCATGAAGAGCAGAAA
AGTTTTAGACCATGCAAAATTAGTAGCAGAGTGTGTTGAGCAACTTGGGCGAATGTTCAAGCCGGATTTTAAAG

>TRINITY_DN45219_c0_g5_i5 from sample A6 / Example unspliced isoform:

TTACTTCAAAGATGGAGGGAATGgtacgcaaaatttgtctattttttaaagtctgtaatgcttacagcttgtgtatttataatttaaaaccctgagaaataatttaaacctccttgatttgtgttgttctaatccatatatttcttctcagCTTACTGATTTGACTTTGGCACGGGAGAACGAACCAAAATTTCAAAAAT
ATCTTGATGATAATGTGCATGCACATCCTGGAATAGAATTGACTGTAACAGTCTTGACTACTGGATTTTGGCCCAGCTACAAGTCATTTGATCTCAATCTGCCTGCTGAGATGGTAAAATGTGTAGAGGTATTTAAAGAATTCTACCAGACACAAACTAAACATCGAAAGCTTACATGGATATACTCTTTAGGGACATGC
AATATTTTTGGCAATTTTAATGCCAGGTCGGTGGAACTTATTGTGACTACATATCAGGTAAATTCTTTGTTTATTTATTTTTATATATATTTTTTATGTTTTCCGTTCTTTTATTTGTTGCCATCCACCTTTTTTCCTGCCAATTTATTGCCATCC

Intron 2 GTGGCTCCTG ... AATACTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	4.69	61	0	3
A2	3.8	76	0	3
A3	7.41	50	0	4
A4	17.54	47	2	10
A5	13.04	20	2	3
A6	0	21	0	0
A7	4.48	64	0	3
A8	1.67	59	0	1
A9	1.56	63	0	1
A10	8.45	65	0	6

>TRINITY_DN107811_c0_g1_i6 from sample A4 / Example spliced isoform

GAGGGAACAACGCTTGTTAAACAAGCAGAAGATGCAGCAAGCAATAAGAAGTCGGAGAAAAAGGATATTGTTGGTCCACTAGAGGAGACATTTGTGTGGGAAGTTATTGGATTGCATGACAAGTACTCGCAGTATATCAGCACGTGCTTTGTGAACCATTCTCTTTTCCACAAGGTTTACTTGCGTATGCTCATTCTCTA
TGTTTTTTATTCCTAAGAATATTTTTTACAGATGTTCTGTATATAAGATTTATTTCATTTTATGAACTTTTGCAGGTCTTAAAAGAAGCATTCGAGGTATTTTGTAACAGAGTAACAGGAAACACAAGTGCAGAGTTATTGGCTACTTTTTGTGACAACCTATTGAAAAAGGGTAGCACTGAAAAATTAAGTGATGAAGA
TATTGAAGAGACACTTGAGAAGGTGGTAAAGTCTTTTGCATACATCGGTGATAAATATCTATTTTCAGAATTTTATAGGTAGGTTTCTCCATATCTTCCTCTTCAAAACTTATTTCCTGAATAAATCAGATATATGTCATAATAAGTAAGATCTTCTCTAGCTTTTGCTGGGCATTTAAAGAAAACTATTGAATGAAAAG
CTTCCAATTTTTTTAGCTTTTTCTGTTTTGAGTTGCGTTAACCTTTTTGTGAGAGAATTTGAGGAATAGATTGCAGATTATTTGTTATTTTTCCTGTGATAATATTTCAAATGTGTGCCAGCTAAGGTTGGCTAACAAACTTTGATGGACAAATAGTATTCAGTGAACAAGGTACATTGTATTTGAACATTGTAGGAAAA
AACTAGCACGTCGGCTTATATTTGATAAAACTACAAATGATGAACATGAACGGAGTATCTTAATGAAATTGAAGCAAAAATGTGGAGGCCAGTTTACTTCAAAGATGGAGGGAATGCTTACTGATTTGACTTTGGCACGGGAGAAC

>TRINITY_DN107811_c0_g1_i3 from sample A4 / Example unspliced isoform:

TGAAGAGACACTTGAGAAGgtggctcctgcatttcatttgtgattactgcaattataaaattacctagcttacttgagtgctgatatcatcattcataaatactgcagGTGGTAAAGTCTTTTGCATACATCGGTGATAAATATCTATTTTCAGAATTTTATAGGTAGGTTTCTCCATATCTTCCTCTTCAAAACTTATT
TCCTGAATAAATCAGATATATGTCATAATAAGTAAGATCTTCTCTAGCTTTTGCTGGGCATTTAAAGAAAACTATTGAATGAAAAGCTTCCAATTTTTTTAGCTTTTTCTGTTTTGAGTTGCGTTAACCTTTTTGTGAGAGAATTTGAGGAATAGATTGCAGATTATTTGTTATTTTTCCTGTGATAATATTTCAAATGT
GTGCCAGCTAAGGTTGGCTAACAAACTTTGATGGACAAATAGTATTCAGTGAACAAGGTACATTGTATTTGAACATTGTAGGAAAAAACTAGCACGTCGGCTTATATTTGATAAAACTACAAATGATGAACATGAACGGAGTATCTTAATGAAATTGAAGCAAAAATGTGGAGGCCAGTTTACTTCAAAGATGGAGGGAA
TGCTTACTGATTTGACTTTGGCACGGGAGAAC

Intron 3 GTCCGAGCTA ... TGCATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.67	135	22	32
A2	18.28	114	21	30
A3	21.34	94	21	30
A4	17.54	87	18	19
A5	14.29	36	2	10
A6	17.22	125	24	28
A7	17.66	282	54	67
A8	15.26	336	46	75
A9	13.85	280	35	55
A10	15.03	229	30	51

>TRINITY_DN45219_c0_g5_i4 from sample A6 / Example spliced isoform

CGCAAAATGTGACTGTTTTTTTAAGTCTGTGTAATGCTTACAGCTTGTGTATTTATAATTTAAAACCCTGAGAAATAATTTAATGCTCTTTGATTCATGTTATTCTAATCCATATATTTCTTCTCAGCTTACTGATTTGACTTTGGCACGGGAGAACGAACCAAAATTTCAAAAATATCTTGATGATAATGTGCATGCAC
ATCCTGGAATAGAATTGACTGTAACAGTCTTGACTACTGGATTTTGGCCCAGCTACAAGTCATTTGATCTCAATCTGCCTGCTGAGATGGTAAAATGTGTAGAGGTATTTAAAGAATTCTACCAGACACAAACTAAACATCGAAAGCTTACATGGATATACTCTTTAGGGACATGCAATATTTTTGGCAATTTTAATGCC
AGGTCGGTGGAACTTATTGTGACTACATATCAGGGATCTGTATTATTGCTGTTCAATTCTGCAGAAAAATTGAGCTACTCAGAAATCAAATCTCAGTTGAATTTGTCTGATGAAGAGGTTGTTAGATTGCTACATTCACTTTCGTGTTTAAAACACAAAGTCTTGAATAAGGAGCCAAACACAAAAACTGTCACACAATC
TGATTACTTCGAATTTAATGCAAAATTTACTGACAAATCAAGAAAGATTAAGATTCCTCTTTCTCCTGTGGATGAGAAGAAGACAATAATTGAGGACGTGGACAAAGATAGACGATATGCAATTGATGCTTGTATTGTTCGAATCATGAAGAGCAGAAAAGTTTTAGACCATGCAAAATTAGTAGCAGAGTGTGTTGAGC
AACTTGGGCGAATGTTCAAGCCGGATTTTAAAG

>TRINITY_DN45219_c0_g5_i7 from sample A6 / Example unspliced isoform:

CGCAAAATGTGACTGTTTTTTTAAGTCTGTGTAATGCTTACAGCTTGTGTATTTATAATTTAAAACCCTGAGAAATAATTTAATGCTCTTTGATTCATGTTATTCTAATCCATATATTTCTTCTCAGCTTACTGATTTGACTTTGGCACGGGAGAACGAACCAAAATTTCAAAAATATCTTGATGATAATGTGCATGCAC
ATCCTGGAATAGAATTGACTGTAACAGTCTTGACTACTGGATTTTGGCCCAGCTACAAGTCATTTGATCTCAATCTGCCTGCTGAGATGGTAAAATGTGTAGAGGTATTTAAAGAATTCTACCAGACACAAACTAAACATCGAAAGCTTACATGGATATACTCTTTAGGGACATGCAATATTTTTGGCAATTTTAATGCC
AGGTCGGTGGAACTTATTGTGACTACATATCAGGGATCTGTATTATTGCTGTTCAATTCTGCAGAAAAATTGAGCTACTCAGAAATCAAATCTCAGTTGAATTTGTCTGATGAAGAGGTTGTTAGATTGCTACATTCACTTTCGTGTTTAAAACACAAAGTCTTGAATAAGGAGCCAAACACAAAAACTGTCACACAATC
TGATTACTTCGAATTTAATGCAAAATTTACTGACAAATCAAGAAAGATTAAGgtccgagctaacaaaccttgattatttgatttcatgtctatcttgtcacattaaaggatatattgataacatcgtttttactgcattgtagATTCCTCTTTCTCCTGTGGATGAGAAGAAGACAATAATTGAGGACGTGGACAAAGAT
AGACGATATGCAATTGATGCTTGTATTGTTCGAATCATGAAGAGCAGAAAAGTTTTAGACCATGCAAAATTAGTAGCAGAGTGTGTTGAGCAACTTGGGCGAATGTTCAAGCCGGATTTTAAAG

Intron 4 GTAGGTTTCT ... AACATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.31	153	7	39
A2	8.62	159	8	15
A3	16.18	171	5	33
A4	25.64	87	10	30
A5	21.05	30	7	8
A6	6.38	44	1	3
A7	18.43	177	10	40
A8	14.09	128	3	21
A9	5.38	176	0	10
A10	21.72	155	17	43

>TRINITY_DN44460_c2_g1_i3 from sample A9 / Example spliced isoform

TACCAATGAGGGAACAACGCTTGTTAAACAAGCAGAAGATACAGCAAGCAATAAGAAGTCAGAGAAAAAGACTATTGTTGGTCCACTAGAGCAGACATTTGTGTGGGAAGCTATTGAATTGCATGACAAGTACTTACAGTATATCAGCACATGCTTTGTGAACCATTCTCTTTTCCACAAGGTCTTAAAAGAAGCATTCG
AGGTATTTTGTAACAGAGTAACAGGAAACACAAGTGCAGAGTTATTGGCTACTTTTTGTGACAACCTATTGAAAAAGGGTAGCACTGAAAAATTAAGTGATGAAGATATTGAAGAGACACTTGAGAAGGTGGTAAAGTCTTTTGCATACATCGGTGATAAATATCTATTTTCAGAATTTTATAGGAAAAAACTAGCACGT
CGGCTTATATTTGATAAAACTACAAATGATGAACATGAACGGAGTATCTTAATGAAATTGAAGCAAAAATGTGGAGGCCAGTTTACTTCAAAGATGGAGGGAATGCTTACTGATTTGACTTTGGCACGGGAGAACGAACCAAAATTTCAAAAATATCTTGATGATAATGTGCATGCACATCCTGGAATAGAATTGAGTGT
AACAGTCTTG

>TRINITY_DN44460_c2_g1_i1 from sample A9 / Example unspliced isoform:

AGTGATGAAGATATTGAAGAGACACTTGAGAAGGTGGTAAAGTCTTTTGCATACATCGGTGATAAATATCTATTTTCAGAATTTTATAGgtaggtttctccatatcttcctcttcaaaacttatttcctgaataaatcagatatatgtcataataagtaagatcttctctagcttttgctgggcatttaaagaaaactat
tgaatgaaaagcttccaatttttttagctttttctgttttgagttgcgttaacctttttgtgagagaatttgaggaatagattgcagattatttgttatttttcctgtgataatatttcaaatgtgtgccagctaaggttggctaacaaactttgatggacaaatagtattcagtgaacaaggtacattgtatttgaaca
ttgtagGAAAAAACTAGCACGTCGGCTTATATTTGATAAAACTACAAATGATGAACATGAACGGAGTATCTTAATGAAATTGAAGCAAAAATGTGGAGGCCAGTTTACTTCAAAGATGGAGGGAATG

Intron 5 GTACGCAAAA ... TTCTTCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.94	159.5	35.5	104.5
A2	38.3	147.5	35	91
A3	32.33	153.5	33.5	72.5
A4	41.86	80	18	57
A5	42.05	41	13	29.5
A6	44.8	55	11.5	44.5
A7	37.39	224.5	44.5	133.5
A8	38.63	189	26	119
A9	28.51	195.5	19	77.5
A10	40.53	201.5	31	137

>TRINITY_DN49100_c4_g1_i2 from sample A7 / Example spliced isoform

CTTACAGTATATCAGCACATGCTTTGTGAACCATTCTCTTTTCCACAAGGTCTTAAAAGAAGCATTCGAGGTATTTTGTAACAGAGTAACAGGAAACACAAGTGCAGAGTTATTGGCTACTTTTTGTGACAACCTATTGAAAAAGGGTAGCACTGAAAAATTAAGTGATGAAGATATTGAAGAGACACTTGAGAAGGTGG
TAAAGTCTTTTGCATACATCGGTGATAAATATCTATTTTCAGAATTTTATAGGAAAAAACTAGCACGTCGGCTTATATTTGATAAAACTACAAATGATGAACATGAACGGAGTATCTTAATGAAATTGAAGCAAAAATGTGGAGGCCAGTTTACTTCAAAGATGGAGGGAATGCTTACTGATTTGACTTTGGCACGGGAG
AACCAACCAAAATTTCAAAAATATCTTGATGATAATGTGCATGCACATCCTGGAATAGAATTGACTGTAACAGTCTTGACTACTGGATTTTGGCCCAGCTACAAGTCATTTGATCTCAATCTGCCTGCTGAGATGGTAAAATGTGTAGAGGTATTTAAAGAATTCTACCAGACACAAACTAAACATCGAAAGCTTACATG
GATATACTCTTTAGGGACATGCAATATTTTTGGCAATTTTAA

>TRINITY_DN49100_c4_g1_i4 from sample A7 / Example unspliced isoform:

CTTACAGTATATCAGCACATGCTTTGTGAACCATTCTCTTTTCCACAAGGTCTTAAAAGAAGCATTCGAGGTATTTTGTAACAGAGTAACAGGAAACACAAGTGCAGAGTTATTGGCTACTTTTTGTGACAACCTATTGAAAAAGGGTAGCACTGAAAAATTAAGTGATGAAGATATTGAAGAGACACTTGAGAAGGTGG
TAAAGTCTTTTGCATACATCGGTGATAAATATCTATTTTCAGAATTTTATAGGAAAAAACTAGCACGTCGGCTTATATTTGATAAAACTACAAATGATGAACATGAACGGAGTATCTTAATGAAATTGAAGCAAAAATGTGGAGGCCAGTTTACTTCAAAGATGGAGGGAATGgtacgcaaaatgtgactgtttttttaa
gtctgtgtaatgcttacagcttgtgtatttataatttaaaaccctgagaaataatttaaacctccttgatttgtgttgttctaatccatatatttcttctcagCTTACTGATTTGACTTTGGCACGGGAGAACCAACCAAAATTTCAAAAATATCTTGATGATAATGTGCATGCACATCCTGGAATAGAATTGACTGTAA
CAGTCTTGACTACTGGATTTTGGCCCAGCTACAAGTCATTTGATCTCAATCTGCCTGCTGAGATGGTAAAATGTGTAGAGGTATTTAAAGAATTCTACCAGACACAAACTAAACATCGAAAGCTTACATGGATATACTCTTTAGGGACATGCAATATTTTTGGCAATTTTAA