sp|Q9LU41|ACA9_ARATH ----> Calcium-transporting ATPase 9, plasma membrane-type

Intron 1 GTATGTGTAT ... AAACTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.37	35	9	23
A2	23.4	18	2	9
A3	26.32	21	10	5
A4	20	12	4	2
A5	10	9	2	0
A6	12.64	38	1	10
A7	19.67	49	10	14
A8	23.68	58	15	21
A9	24.41	48	22	9
A10	17.24	48	7	13

>TRINITY_DN41726_c2_g1_i13 from sample A8 / Example spliced isoform

CCAGGTAGGACCGCCAGAAGAACTATCTGTAATTGAAGGCAGGGTTTTTCGCAACTATACTAAAGAAGAGCAACTGGAAATTGTGGACAAAATTTGTGTAATGGGAAGATGCTCACCTACAGACAAACTTCACTTCGTGCAAGCATTGAAAAAAAGAGGTGATGTTGTGGCGGTCACTGGAGATGGAACGAATGATGCGC
CTGCACTTCATGAGGCAGACATAGGTCTCTCCATGGGCATCCAAGGAACGGAAGTTGCCAAGGAAAGCTCTGATATAATTATTCTAGATGATAATTTTGCCTCGGTTGTGAAGGTTGTTCGGTGGGGCCGTTCAGTTTATGCAAACATTCAGAAGTTCATTCAATTTCAACTTACTCTCAGCATTGCAGCAATTGGGACT
AATTTTGTTGCTGCTGTTTCGTCTGGAGATGTTCCTTTGTCTACTGTTCAGCTGCTTTGGGTCAACCTTATCCAGGATACTCTTGGAGCTCTTGCATTGGCAACCGAATCTCCAACTGATCTCCTCATGCACCGGCCTCCAGTTGGGCACAGGGAACCCATTATTACAAACGTTATGTGGAGAAATATTATGGCTCAAGG
TTTGTACCAAGTTGCAGTCCTCCTTGTACTTACATTTAAGGGCATGGACATTTTAAAGCTAAAGCATGATAACACAGGCCATGCAGTCACAGTGAGAAACACTCTAATTTTTAATGCCTTTGTCTTTTGCCAAATATTCAATGAATTCAATTCAAG

>TRINITY_DN41726_c2_g1_i7 from sample A8 / Example unspliced isoform:

CCAGGTAGGACCGCCAGAAGAACTATCTGTAATTGAAGGCAGGGTTTTTCGCAACTATACTAAAGAAGAGCAACTGGAAATTGTGGACAAAATTTGTGTAATGGGAAGATGCTCACCTACAGACAAACTTCACTTCGTGCAAGCATTGAAAAAAAGAGGTGATGTTGTGGCGGTCACTGGAGATGGAACGAATGATGCGC
CTGCACTTCATGAGGCAGACATAGGTCTCTCCATGGGCATCCAAGGAACGGAAGTTGCCAAGGAAAGCTCTGATATAATTATTCTAGATGATAATTTTGCCTCGGTTGTGAAGGTTGTTCGGTGGGGCCGTTCAGTTTATGCAAACATTCAGAAGTTCATTCAATTTCAACTTACTCTCAGCATTGCAGCAATTGGGACT
AATTTTGTTGCTGCTGTTTCGTCTGGAGATGTTCCTTTGTCTACTGTTCAGCTGCTTTGGGTCAACCTTATCCAGGATACTCTTGGAGCTCTTGCATTGGCAACCGAATCTCCAACTGATCTCCTCATGCACCGGCCTCCAGTTGGGCACAGGGAACCCATTATTACAAACGTTATGTGGAGAAATATTATGGCTCAAgt
atgtgtattcacttgccttttggtagtgattaagaaatctagtatggtttccaaatataacagtgaataagaaggcgagtattttctcagattataatagtaggtaaataattttaaggtgcactgatggctctggaaaacttgtagGGTTTGTACCAAGTTGCAGTCCTCCTTGTACTTACATTTAAGGGCATGGACAT
TTTAAAGCTAAAGCATGATAACACAGGCCATGCAGTCACAGTGAGAAACACTCTAATTTTTAATGCCTTTGTCTTTTGCCAAATATTCAATGAATTCAATTCAAG

Intron 2 GTTTGCATGG ... ATTTACACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	94.44	1	17	17
A2	86.96	3	20	20
A3	68.75	5	12	10
A4	60	5	7	8
A5	100	0	5	5
A6	77.78	7	23	26
A7	68.75	20	35	53
A8	84	6	30	33
A9	78.95	6	18	27
A10	78.02	10	33	38

>TRINITY_DN42398_c4_g1_i4 from sample A6 / Example spliced isoform

TTCATTTTTATTTTTAAATTTTGAGTTGCTCACGAATGTAGCCATCGTGTTGTAGGGGATCTACATTTTGAACAAAAACCTCACAGCAAAAGCTCATTCGCCGGAGGTTTTGGATCCACCAAAAGCTTCTCCCATTTTAGCCCTTGCATGAGCAGTTTCTATGCATTGGCTTTATTCAACTTGCATCTGAGGGAGTTACT
AAGCAAATGGGCACGCCTTTGCCTTAACTCAAGAGAAGAAGGGTTTGAGTTTTTAGGGGCCTGACCTTCTCGGGTGTCTTTCATTATCCCTGGAATTATGGATTTGCACACTCTCGGATCTATAGGGGTACTGAACGACAGATTGATGCAAGCCATGTTGGATAGTTGGGCGATCCAAAACTTCATGAAGGAAAGTGTTG
TGAGGGAGCTTGGGTTTCAGCTTGAGCCTGTGCAAACTTATTTCAAAGCATTGAACTCATAAGTGGCTAAGGATCCTGCGTTGCCAGGGAAATTTTGTACCATGGTCCAGCAAAATCTATAGCTCTTGAATGTGGCATACTCCAGACAAGACAACCTACAGAAGAGCTAACTATCATTGAAGGACCAATTTTTCGCAGCT
ACACTGAAGAAGAGAGAATGAAAATTGTGGATAAAATTTGTAATGGGAAGGTCCTCTCCTATAGACAAGACTTACTTTGTTCAAGCATTGAGAAAAAGAGGTCATGTTGTTGCAGTGACTGGAGATGCAACAAATGATGCACCTGCACTTCATGAGGAAGACATAGGTCTCTCCATGGGTACCCAAGGAAAGCTCATATA
TCATTATTCTAGAGGATAATTTTGCCTCCATTGTGAAGCAATTGCGATTAATTTTGTTGCTGCCGTTTCATCTGGAAATGTTCCATTATCTGAGGTTCAGCTTCTTTAGGTCAACCTTATCATGGATACTCTTGGAGCACTTACATTGGAAACTGAATCCCCAATTGATCATCTCATGCACCGCCCTCCAATAGAGCGCA
GGTAGGACATTGTCATCAAGAGCAGTTCCTCCTTTGGATTAAAAGCTATATAATTCTTGCAGATGCCAAAATATTTTGAAATAAAGAGCATGGGACTAACAGTGGATAAGTACACATACTTCTGTTCAAAATGAGGAACAACTAGATGCATGTACCAAGCCCACACTACAATCAATTGATATTATTAGCATGTACTTGTG
CAAATTTGATATAAATGATAGCTAGCATAAAGGTATGCTTTTGAAGAAG

>TRINITY_DN42398_c4_g1_i8 from sample A6 / Example unspliced isoform:

TTCATTTTTATTTTTAAATTTTGAGTTGCTCACGAATGTAGCCATCGTGTTGTAGGGGATCTACATTTTGAACAAAAACCTCACAGCAAAAGCTCATTCGCCGGAGGTTTTGGATCCACCAAAAGCTTCTCCCATTTTAGCCCTTGCATGAGCAGTTTCTATGCATTGGCTTTATTCAACTTGCATCTGAGGGAGTTACT
AAGCAAATGGGCACGCCTTTGCCTTAACTCAAGAGAAGAAGGGTTTGAGTTTTTAGGGGCCTGACCTTCTCGGGTGTCTTTCATTATCCCTGGAATTATGGATTTGCACACTCTCGGATCTATAGGGGTACTGAACGACAGATTGATGCAAGCCATGTTGGATAGTTGGGCGATCCAAAACTTCATGAAGGAAAGTGTTG
TGAGGGAGCTTGGGTTTCAGCTTGAGCCTGTGCAAACTTATTTCAAAGCATTGAACTCATAAGTGGCTAAGGATCCTGCGTTGCCAGGGAAATTTTGTACCATGGTCCAGgtttgcatggtgactggtgataacatttacacagCAAAATCTATAGCTCTTGAATGTGGCATACTCCAGACAAGACAACCTACAGAAGAG
CTAACTATCATTGAAGGACCAATTTTTCGCAGCTACACTGAAGAAGAGAGAATGAAAATTGTGGATAAAATTTGTAATGGGAAGGTCCTCTCCTATAGACAAGACTTACTTTGTTCAAGCATTGAGAAAAAGAGGTCATGTTGTTGCAGTGACTGGAGATGCAACAAATGATGCACCTGCACTTCATGAGGAAGACATAG
GTCTCTCCATGGGTACCCAAGGAAAGCTCATATATCATTATTCTAGAGGATAATTTTGCCTCCATTGTGAAGCAATTGCGATTAATTTTGTTGCTGCCGTTTCATCTGGAAATGTTCCATTATCTGAGGTTCAGCTTCTTTAGGTCAACCTTATCATGGATACTCTTGGAGCACTTACATTGGAAACTGAATCCCCAATT
GATCATCTCATGCACCGCCCTCCAATAGAGCGCAGGTAGGACATTGTCATCAAGAGCAGTTCCTCCTTTGGATTAAAAGCTATATAATTCTTGCAGATGCCAAAATATTTTGAAATAAAGAGCATGGGACTAACAGTGGATAAGTACACATACTTCTGTTCAAAATGAGGAACAACTAGATGCATGTACCAAGCCCACAC
TACAATCAATTGATATTATTAGCATGTACTTGTGCAAATTTGATATAAATGATAGCTAGCATAAAGGTATGCTTTTGAAGAAG

Intron 3 GTGAGTATGG ... TTGCCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	6.38	22	1	2
A2	20.69	23	8	4
A3	9.38	29	2	4
A4	17.24	12	3	2
A5	0	5	0	0
A6	17.95	16	4	3
A7	31.11	31	18	10
A8	42.86	20	12	18
A9	36.51	20	16	7
A10	18.07	34	13	2

>TRINITY_DN41726_c2_g1_i4 from sample A8 / Example spliced isoform

GGGAAATTTTGTACCATGGTCCAGGTTTGCATGGTGACTGGTGATAACATTTACACAGCAAAATCTATAGCTCTTGAATGTGGCATACTCCAGACAAGACAACCTACAGAAGAGCTAACTATCATTGAAGGACCAATTTTTCGCATCTACACTGAAGAAGAGAGAATGAAAATTGTGGATAAAATTTGTAATGGGAAGGT
CCTCTCCTATAGACAAGACTTACTTTGTTCAAGCATTGAGAAAAAGAGGTCATGTTGTTGCAGTGACTGGAGATGCAACAAATGATGCACCTGCACTTCATGAGGAAGACATAGGTCTCTCCATGGGTACCCAAGGAAAGCTCATATATCATTATTCTAGAGGATAATTTTGCCTCCATTGTGAAGCAATTGCGATTAAT
TTTGTTGCTGCCGTTTCATCTGGAAATGTTCCATTATCTGAGGTTCAGCTTCTTTAGGTCAACCTTATCATGGATACTCTTGGAGCACTTACATTGGAAACTGAATCCCCAATTGATCATCTCATGCACCGCCCTCTAATAGAGTGCAGGTAGGACATTGTTATCAAGAGCAGTTCCTTAAAATCTATATAATTCTTGCA
GATGCCAAAATATTTTGAAATAAAGAGCATGGGACTAACAGTGGATAAGTACACATACTTCTGTTCAAAATGAGGAACAACTAGATGCATGTACCAAGCCCACACTACAATCAATTGATATTATTAGCATGTACTTGTGCAAATTTGATATAAATGATAGCTAGCATAAAGGTATGCTTTTGAAGAAGAAAACTGCATAG
TGTCAAATGCATGCTCACAAGTCAAGTTTTCCATATTTAAATATTTCACCAGTTACTCTGATATACTGTTTCCTTCCATCCAAAGAGCTACATACAGGATAATCGTTCTCAGTTATCTGGCAGACCAAACACCGTTTGCTTCTTTTAATGGTGTGTTGTTTGTAGACATTAATGGAAATTTCAACACTGCAGATGAACTC
GCAAATTTTTATGGCCAAGTGAATTGTATATAGAAGTTTGTATTTTGCAAGGATATAAATACTCATGCCTATCATGTATTTATTTGAAAATTTTGTTGCATATGTATC

>TRINITY_DN41726_c2_g1_i1 from sample A8 / Example unspliced isoform:

GGGAAATTTTGTACCATGGTCCAGGTTTGCATGGTGACTGGTGATAACATTTACACAGCAAAATCTATAGCTCTTGAATGTGGCATACTCCAGACAAGACAACCTACAGAAGAGCTAACTATCATTGAAGGACCAATTTTTCGCATCTACACTGAAGAAGAGAGAATGAAAATTGTGGATAAAATTTgtgagtatggatg
tacctgtcccctgccaacacaacacacaaaaaaataagggaaatgcatacctacaacagcttcttacaaaataaaattgtaatggaagggacattaatttgtttgcctgcagGTAATGGGAAGGTCCTCTCCTATAGACAAGACTTACTTTGTTCAAGCATTGAGAAAAAGAGGTCATGTTGTTGCAGTGACTGGAGATG
CAACAAATGATGCACCTGCACTTCATGAGGCGGGTTTTCTTGTTGCATACTGTTGTAAATTCTTATTATGTAAGTAGGTTTTCCAGTGCAATGATATTTCCAGTTCTATAGAGTTATTGATGGAACTTGTTGATATATTATTTGGTGTTTAGGAAGACATAGGTCTCTCCATGGGTACCCAAGGAAAGCTCATATATCAT
TATTCTAGAGGATAATTTTGCCTCCATTGTGAAGGTTTGTTGCAGACATAGATATTGTTTTAATCCTTTAAAGCCACG