NA ----> NA

Intron 1 GTAAATTGAT ... AATTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.05	11.9	0.6	5
A2	35.71	9.1	2	5
A3	0	19	0	0
A4	14.8	17.3	0.9	3
A5	40	3	0	2
A6	24.43	126.9	4	41
A7	22.46	183	7	53
A8	25.91	137.3	2.9	48
A9	22.52	161.7	7.4	47
A10	24.32	146.3	6.3	47

>TRINITY_DN104936_c0_g1_i2 from sample A2 / Example spliced isoform

TCATTCACGAGTAAATAAGGTTCCGCGGATACAGAGACGCGATGGTGAGAGAAGCAACCGTGAAAAGAGAAGGGCAGGATTCTTTAAAATAATCTGACTAGACGGCAGACGCATATTACTCGGACTGAAGAAGAGAAGGCACGAAGGAAGGATGGTTTATTCATAATGAAGATAGAAGCAACTGCAGAGTATCATCCAGA
AGAAGGTATTTCTTATGCATACATCAGAAATCAGATAGAAGCAATTCCAGGACCGTCTAGCAGTAGAAGGTGACAGGGCTAAATGTAAGCTGTGATTTATGTTAAGTGGTTGTATCATTGTCAGCACAGAATACTATTTGTGGTTGGCACTATTGCCTGGATGAAATGAGGGCACTTATGGATGCACATACAGAAAGGGA
GAGAACAGTATTTGTGACAGTTGGTACTACTTCTTTTGACACCCTTGTCAAGGCAGTTGACACTATCCAATGTAAAGCAGCCCTAGCAAGCAAAGGGTACACCAATCTGCTTATCCAACTAGGCCGTGGAACATATTTCCCAACCAAGTCTCCAGGGGAAGATGGAGGTCTGAAAGTTGATTACTTCACTTTTTCACCAA
ATATTGCTGGGTTTTTGAGATCTGCAGCCTTGGTGATTAGTCATGCAGGTTCTGGCAGTATATTTGAAACTCTCCGTTTAGGTCAGCCACTTGTTGTCGTTGTGAATGAAGCATTGATGGATAATCATCAAAGTGAATTAGCTGAAGAACTTGCAGAGAGAAAGTTCTTATTTTGTGCTCGCCCCGAAACTCTAGAAGAA
ACTATACTACGCATGGATCCAGGGTCACTCCTCCCTTATCCACTATCAAGTCCTATGGCAGTAGTTCAATCAATCAACCAGTTTTTAGGCTTTCCTGACGACTGAGGTCCAGGGCAAGTTTGTTTCATGTGACCCCTGTAAAACATTTTACAACTGCTTCTATAAACCAGAATAGTAAGAAATGGTAAAGTTGCAGCCTC
AATTTCAATGCCCATTGATTGTCGAACTTCAATATTATGTGTCCGCATGTCTATTTTCTAGTTAAAAGCATGCTATGACTTGATAATCAATTATGTTTTATGAGATGATCAGTGCTAAAACTATTATTTTGTTTCAAGGG

>TRINITY_DN104936_c0_g1_i3 from sample A2 / Example unspliced isoform:

TCATTCACGAGTAAATAAGGTTCCGCGGATACAGAGACGCGATGGTGAGAGAAGCAACCGTGAAAAGAGAAGGGCAGGATTCTTTAAAATAATCTGACTAGACGGCAGACGCATATTACTCGGACTGAAGAAGAGAAGGCACGAAGGAAGGATGGTTTATTCATAATGAAGATAGAAGCAACTGCAGAGTATCATCCAGA
AGAAGGTATTTCTTATGCATACATCAGAAATCAGATAGAAGCAATTCCAGGACCGTCTAGCAGTAGAAGGTGACAGGGCTAAATGTAAGCTGTGATTTATGTTAAGTGGTTGTATCATTGTCAGCACAGAATACTATTTGTGGTTGGCACTATTGCCTGGATGAAATGAGGGCACTTATGGATGCACATACAGAAAGGGA
GAGAACAGTATTTGTGACAGTTGGTACTACTTCTTTTGACACCCTTGTCAAGGCAGTTGACACTATCCAATGTAAAGCAGCCCTAGCAAGCAAAGGGTACACCAATCTGCTTATCCAACTAGGCCGTGGAACATATTTCCCAACCAAGTCTCCAGGGGAAGATGGAGGTCTGAAAGTTGATTACTTCACTTTTTCACCAA
ATATTGCTGGGTTTTTGAGATCTGCAGCCTTGGTGATTAGTCATGCAGgtaaattgatattttagaaattatgaagagattacaaccatatcttaattttattgtataaagtaattagtttttgacatcatgataatcactgtaaaagactttaagaattgtgcagGTTCTGGCAGTATATTTGAAACTCTCCGTTTAGG
TCAGCCACTTGTTGTCGTTGTGAATGAAGCATTGATGGATAATCATCAAAGTGAATTAGCTGAAGAACTTGCAGAGAGAAAGTTCTTATTTTGTGCTCGCCCCGAAACTCTAGAAGAAACTATACTACGCATGGATCCAGGGTCACTCCTCCCTTATCCACTATCAAGTCCTATGGCAGTAGTTCAATCAATCAACCAGT
TTTTAGGCTTTCCTGACGACTGAGGTCCAGGGCAAGTTTGTTTCATGTGACCCCTGTAAAACATTTTACAACTGCTTCTATAAACCAGAATAGTAAGAAATGGTAAAGTTGCAGCCTCAATTTCAATGCCCATTGATTGTCGAACTTCAATATTATGTGTCCGCATGTCTATTTTCTAGTTAAAAGCATGCTATGACTTG
ATAATCAATTATGTTTTATGAGATGATCAGTGCTAAAACTATTATTTTGTTTCAAGGG

Intron 2 GTGACAGGGC ... CCCAACCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.92	3	9	10
A2	81.48	5	5	22
A3	82.61	4	5	19
A4	76.92	6	6	20
A5	78.57	3	0	11
A6	81.34	25	10	109
A7	73.49	44	21	122
A8	80.52	30	21	124
A9	83.87	25	24	130
A10	80.39	30	24	123

>TRINITY_DN95590_c0_g1_i6 from sample A4 / Example spliced isoform

AAGTAACTCCACTCGGGAATTAGTGCCTAAACCACCTCCCACTTCCCAAATTGAGCCAAAAAAAATACTGATGGTCTGTCAGCTTTAATACCATCCCTCCTCCACAACGCTCCATATTGGAGGCTCCATCGAAGTAGCCACATGGGACTCCTAACACAGGAACAGGTTCCTTAACAGCACGAGTACCTTTGAGGGTCACT
GGCTTAAAAAATTCCTTAAAAAACCTGTAGGCAGATCCCGATTCTTAAGTCTCCAGGGGAAGATGGAGGCCTAAAAGTTGATTACTTCACTTTTTCACCAAATATTGCTGGGTTTTTGAGATCTGCAGCCTTGGTGATTAGTCATGCAGGTTCTGGCAGTATATTTGAAACTCTCCGTTTAGGTCAGCCACTTGTTGTCG
TTGTGAATGAAGCATTGATGGATAATCATCAAAGTGAATTAGCTGAAGAACTTGCAGAGAGAAAGTTCTTATTTTGTGCTCGCCCCGAAACTCTAGAAGAAACTATACTACGCATGGATCCAGGGTCACTCCTCCCTTATCCACTATCAAGTCCTATGGCAGTAGTTCAATCAATCAACCAGTTTTTAGGCTTTCCTGAC
GACTGAGGTCCAGGGCAAGTTTGTTTCATGTGACCCCTGTAAAACATTTTACAACTGCTTCTATAAACCAGAATAGTAAGAAATGGTAAAGTTGCAGCCTCAATTTCAATGCCCATTGATTGTCGAACTTCAATATTATGTGTCCGCATGTCTATTTTCTAGTTAAAAGCATGCTATGACTTGATAATCAATTATGTTTT
ATGAGATGATCAGTGCT

>TRINITY_DN95590_c0_g1_i2 from sample A4 / Example unspliced isoform:

AAGTAACTCCACTCGGGAATTAGTGCCTAAACCACCTCCCACTTCCCAAATTGAGCCAAAAAAAATACTGATGGTCTGTCAGCTTTAATACCATCCCTCCTCCACAACGCTCCATATTGGAGGCTCCATCGAAGTAGCCACATGGGACTCCTAACACAGGAACAGGTTCCTTAACAGCACGAGTACCTTTGAGGGTCACT
GGCTTAAAAAATTCCTTAAAAAACCTGTAGGCAGATCCCGATTCTTAAGgtgacagggctaaatgtaagttgtgattcatgttaagtggatatatcattgttagtacagaatactctttgtgattggcactaattcctggatgaaatgagggcacttatggatgcacatagagaaagagggagaacagtgtttgtgacag
ttggtactacttcttttgacacccttgtcaaggcagttgacactatccaatgtaaagcagccctagcaagcaaagggtacaccaatctgcttatccaactaggccgtggaacatatttcccaaccaagTCTCCAGGGGAAGATGGAGGCCTAAAAGTTGATTACTTCACTTTTTCACCAAATATTGCTGGGTTTTTGAGA
TCTGCAGCCTTGGTGATTAGTCATGCAGGTTCTGGCAGTATATTTGAAACTCTCCGTTTAGGTCAGCCACTTGTTGTCGTTGTGAATGAAGCATTGATGGATAATCATCAAAGTGAATTAGCTGAAGAACTTGCAGAGAGAAAGTTCTTATTTTGTGCTCGCCCCGAAACTCTAGAAGAAACTATACTACGCATGGATCC
AGGGTCACTCCTCCCTTATCCACTATCAAGTCCTATGGCAGTAGTTCAATCAATCAACCAGTTTTTAGGCTTTCCTGACGACTGAGGTCCAGGGCAAGTTTGTTTCATGTGACCCCTGTAAAACATTTTACAACTGCTTCTATAAACCAGAATAGTAAGAAATGGTAAAGTTGCAGCCTCAATTTCAATGCCCATTGATT
GTCGAACTTCAATATTATGTGTCCGCATGTCTATTTTCTAGTTAAAAGCATGCTATGACTTGATAATCAATTATGTTTTATGAGATGATCAGTGCT

Intron 3 GCATGGCCTT ... TTGGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.57	10	7	1
A2	25.42	22	10	5
A3	33.33	19	15	4
A4	28.57	20	8	8
A5	38.89	11	7	7
A6	24.38	105.5	44	24
A7	28.4	121	57	39
A8	25.37	125	49	36
A9	27.02	131	71	26
A10	25.38	122	59	24

>TRINITY_DN37493_c0_g1_i3 from sample A8 / Example spliced isoform

TATAGCCCATGAAACCTTTGAGTCCATGAACTTTGAAGAAGTTTCAAATTTAAAGAGCCAAAGTTCATACCTCGAGTGAAGAGAGACTATAAAAGCTTTGGCAAACCTATTGAATATCACGACTCATTCACGAGTAAATAAGGTTCCGCGGATACAGAGACGCGATGGTGAGAGAAGCAACCGTGAAAAGAGAAGGGCAG
GATTCTTTAAAATAATCTGACTAGACGGCAGACGCATATTACTCGGACTGAAGAAGAGAAGGCACGAAGGAAGGATGGTTTATTCATAATGAAGATAGAAGCAACTGCAGAGTATCATCCAGAAGAAGGTATTTCTTATGCATACATCAGAAATCAGATAGAAGCAATTCCAGGACCGTCTAGCAGTAGAAGGTGACAGG
GCTAAATGTAAGCTGTGATTTATGTTAAGTGGTTGTATCATTGTCAGCACAGAATACTATTTGTGGTTGGCACTATTGCCTGGATGAAATGAGGGCACTTATGGATGCACATACAGAAAGGGAGAGAACAGTATTTGTGACAGTTGGTACTACTTCTTTTGACACCCTTGTCAAGGCAGTTGACACTATCCAATGTAAAG
CAGCCCTAGCAAGCAAAGGGTACACCAATCTGCTTATCCAACTAGGCCGTGGAACATATTTCCCAACCAAGTCTCCAGGGGAAGATGGAGGTCTGAAAGTTGATTACTTCACTTTTTCACCAAATATTGCTGGGTTTTTGAGATCTGCAGCCTTGGTGATTAGTCATGCAGGTTCTGGCAGTATATTTGAAACTCTCCGT
TTAGGTCAGCCACTTGTTGTCGTTGTGAATGAAGCATTGATGGATAATCATCAAAGTGAATTAGCTGAAGAACTTGCAGAGAGAAAGTTCTTATTTTGTGCTCGCCCCGAAACTCTAGAAGAAACTATACTACGCATGGATCCAGGGTCACTCCTCCCTTATCCACTATCAAATCCTATGGCAGTAGTTCAATCAATCAA
CCAGTTTTTAGGCTTTCCTGACGACTGAGGTCCAGGGCAAGTTTGTTTCATGTGACCCCTGTAAAACATTTTACAACTGCTTCTATAAACCAGAATAGTAAGAAATGGTAAAGTTGCAGCCTCAATTTCAATGCCCATTGATTGTCGAACTTCAATATTATGTGTCCGCATGTCTATTTTCTAGTTAAAAGCATGCTATG
ACTTGATAATCAATTATGTTTTATGAGATGATCAGTGCTAAAACTATTATTTTGTTTCAAGGGGTATTCTGTATGGGAGGTGCAGAAGATTGGAATTATAGTGTATATATATGTGTTGTTTGTCATGGTTGTGAGAATTCAACAAGGCATGTTGAGAAAAGTTTTTATTTTTATTTTTTTACTGTAAATGATTTTAGAGA
CAATGTTATTGCCAAGAGAAACAACATTCAATTCATTAGCGATCCCAAAGTTTATTGCAC

>TRINITY_DN37493_c0_g1_i2 from sample A8 / Example unspliced isoform:

TATAGCCCATGAAACCTTTGAGTCCATGAACTTTGAAGAAGTTTCAAATTTAAAGAGCCAAAGTTCATACCTCGAGTGAAGAGAGACTATAAAAGCTTTGGCAAACCTATTGAATATCACGACTCATTCACGAGTAAATAAGGTTCCGCGGATACAGAGACGCGATGGTGAGAGAAGCAACCGTGAAAAGAGAAGGGCAG
GATTCTTTAAAATAATCTGACTAGACGGCAGACGCATATTACTCGGACTGAAGAAGAGAAGGCACGAAGGAAGGATGGTTTATTCATAATGAAGATAGAAGCAACTGCAGAGTATCATCCAGAAGAAGGTATTTCTTATGCATACATCAGAAATCAGATAGAAGCAATTCCAGGACCGTCTAGCAGTAGAAGGTGACAGG
GCTAAATGTAAGCTGTGATTTATGTTAAGTGGTTGTATCATTGTCAGCACAGAATACTATTTGTGGTTGGCACTATTGCCTGGATGAAATGAGGGCACTTATGGATGCACATACAGAAAGGGAGAGAACAGTATTTGTGACAGTTGGTACTACTTCTTTTGACACCCTTGTCAAGGCAGTTGACACTATCCAATGTAAAG
CAGCCCTAGCAAGCAAAGGGTACACCAATCTGCTTATCCAACTAGGCCGTGGAACATATTTCCCAACCAAGgcatggccttcctcataaatgttttgtattgtatcaacgaagttgatttattgaagaaatatactaatcttgtatctgaggcagtcaaattgtcaaaatctatattttcaccgtcatatcatcacttta
agtccaagtccatggataagatgagccttttgacttgtaagattgccttctaattgattggtcttcccagttatatatgcttttagatctggtgtgcttgttcttttgaattatttagttggaagcagttttctttgttgatggatttttggactaaatctttggttgcagTCTCCAGGGGAAGATGGAGGTCTGAAAGT
TGATTACTTCACTTTTTCACCAAATATTGCTGGGTTTTTGAGATCTGCAGCCTTGGTGATTAGTCATGCAGGTTCTGGCAGTATATTTGAAACTCTCCGTTTAGGTCAGCCACTTGTTGTCGTTGTGAATGAAGCATTGATGGATAATCATCAAAGTGAATTAGCTGAAGAACTTGCAGAGAGAAAGTTCTTATTTTGTG
CTCGCCCCGAAACTCTAGAAGAAACTATACTACGCATGGATCCAGGGTCACTCCTCCCTTATCCACTATCAAATCCTATGGCAGTAGTTCAATCAATCAACCAGTTTTTAGGCTTTCCTGACGACTGAGGTCCAGGGCAAGTTTGTTTCATGTGACCCCTGTAAAACATTTTACAACTGCTTCTATAAACCAGAATAGTA
AGAAATGGTAAAGTTGCAGCCTCAATTTCAATGCCCATTGATTGTCGAACTTCAATATTATGTGTCCGCATGTCTATTTTCTAGTTAAAAGCATGCTATGACTTGATAATCAATTATGTTTTATGAGATGATCAGTGCTAAAACTATTATTTTGTTTCAAGGGGTATTCTGTATGGGAGGTGCAGAAGATTGGAATTATA
GTGTATATATATGTGTTGTTTGTCATGGTTGTGAGAATTCAACAAGGCATGTTGAGAAAAGTTTTTATTTTTATTTTTTTACTGTAAATGATTTTAGAGACAATGTTATTGCCAAGAGAAACAACATTCAATTCATTAGCGATCCCAAAGTTTATTGCAC

Intron 4 GTATTTCTTA ... GCAGTAGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.28	8.7	28	30
A2	79.21	10.7	38	37
A3	72.75	8.7	23	21
A4	68.78	18	38	39
A5	72.53	4.7	14	9
A6	80.04	32.7	130	123
A7	77.27	64	213	213
A8	75.4	52.7	160	151
A9	74.93	76.7	235	200.3
A10	76.64	83	271	258

>TRINITY_DN37493_c0_g1_i4 from sample A8 / Example spliced isoform

CAGAGTATCATCCAGAAGAAGGTGACAGGGCTAAATGTAAGCTGTGATTTATGTTAAGTGGTTGTATCATTGTCAGCACAGAATACTATTTGTGGTTGGCACTATTGCCTGGATGAAATGAGGGCACTTATGGATGCACATACAGAAAGGGAGAGAACAGTATTTGTGACAGTTGGTACTACTTCTTTTGACACCCTTGT
CAAGGCAGTTGACACTATCCAATGTAAAGCAGCCCTAGCAAGCAAAGGGTACACCAATCTGCTTATCCAACTAGGCCGTGGAACATATTTCCCAACCAAGTCTCCAGGGGAAGATGGAGGTCTGAAAGTTGATTACTTCACTTTTTCACCAAATATTGCTGGGTTTTTGAGATCTGCAGCCTTGGTGATTAGTCATGCAG
GTTCTGGCAGTATATTTGAAACTCTCCGTTTAGGTCAGCCACTTGTTGTCGTTGTGAATGAAGCATTGATGGATAATCATCAAAGTGAATTAGCTGAAGAACTTGCAGAGAGAAAGTTCTTATTTTGTGCTCGCCCCGAAACTCTAGAAGAAACTATACTACGCATGGATCCAGGGTCACTCCTCCCTTATCCACTATCA
AATCCTATGGCAGTAGTTCAATCAATCAACCAGTTTTTAGGCTTTCCTGACGACTGAGGTCCAGGGCAAGTTTGTTTCATGTGACCCCTGTAAAACATTTTACAACTGCTTCTATAAACCAGAATAGTAAGAAATGGTAAAGTTGCAGCCTCAATTTCAATGCCCATTGATTGTCGAACTTCAATATTATGTGTCCGCAT
GTCTATTTTCTAGTTAAAAGCATGCTATGACTTGATAATCAATTATGTTTTATGAGATGATCAGTGCTAAAACTATTATTTTGTTTCAAGGGGTATTCTGTATGGGAGGTGCAGAAGATTGGAATTATAGTGTATATATATGTGTTGTTTGTCATGGTTGTGAGAATTCAACAAGGCATGTTGAGAAAAGTTTTTATTTT
TATTTTTTTACTGTAAATGATTTTAGAGACAATGTTATTGCCAAGAGAAACAACATTCAATTCATTAGCGATCCCAAAGTTTATTGCAC

>TRINITY_DN37493_c0_g1_i2 from sample A8 / Example unspliced isoform:

TATAGCCCATGAAACCTTTGAGTCCATGAACTTTGAAGAAGTTTCAAATTTAAAGAGCCAAAGTTCATACCTCGAGTGAAGAGAGACTATAAAAGCTTTGGCAAACCTATTGAATATCACGACTCATTCACGAGTAAATAAGGTTCCGCGGATACAGAGACGCGATGGTGAGAGAAGCAACCGTGAAAAGAGAAGGGCAG
GATTCTTTAAAATAATCTGACTAGACGGCAGACGCATATTACTCGGACTGAAGAAGAGAAGGCACGAAGGAAGGATGGTTTATTCATAATGAAGATAGAAGCAACTGCAGAGTATCATCCAGAAGAAGgtatttcttatgcatacatcagaaatcagatagaagcaattccaggaccgtctagcagtagaagGTGACAGG
GCTAAATGTAAGCTGTGATTTATGTTAAGTGGTTGTATCATTGTCAGCACAGAATACTATTTGTGGTTGGCACTATTGCCTGGATGAAATGAGGGCACTTATGGATGCACATACAGAAAGGGAGAGAACAGTATTTGTGACAGTTGGTACTACTTCTTTTGACACCCTTGTCAAGGCAGTTGACACTATCCAATGTAAAG
CAGCCCTAGCAAGCAAAGGGTACACCAATCTGCTTATCCAACTAGGCCGTGGAACATATTTCCCAACCAAGGCATGGCCTTCCTCATAAATGTTTTGTATTGTATCAACGAAGTTGATTTATTGAAGAAATATACTAATCTTGTATCTGAGGCAGTCAAATTGTCAAAATCTATATTTTCACCGTCATATCATCACTTTA
AGTCCAAGTCCATGGATAAGATGAGCCTTTTGACTTGTAAGATTGCCTTCTAATTGATTGGTCTTCCCAGTTATATATGCTTTTAGATCTGGTGTGCTTGTTCTTTTGAATTATTTAGTTGGAAGCAGTTTTCTTTGTTGATGGATTTTTGGACTAAATCTTTGGTTGCAGTCTCCAGGGGAAGATGGAGGTCTGAAAGT
TGATTACTTCACTTTTTCACCAAATATTGCTGGGTTTTTGAGATCTGCAGCCTTGGTGATTAGTCATGCAGGTTCTGGCAGTATATTTGAAACTCTCCGTTTAGGTCAGCCACTTGTTGTCGTTGTGAATGAAGCATTGATGGATAATCATCAAAGTGAATTAGCTGAAGAACTTGCAGAGAGAAAGTTCTTATTTTGTG
CTCGCCCCGAAACTCTAGAAGAAACTATACTACGCATGGATCCAGGGTCACTCCTCCCTTATCCACTATCAAATCCTATGGCAGTAGTTCAATCAATCAACCAGTTTTTAGGCTTTCCTGACGACTGAGGTCCAGGGCAAGTTTGTTTCATGTGACCCCTGTAAAACATTTTACAACTGCTTCTATAAACCAGAATAGTA
AGAAATGGTAAAGTTGCAGCCTCAATTTCAATGCCCATTGATTGTCGAACTTCAATATTATGTGTCCGCATGTCTATTTTCTAGTTAAAAGCATGCTATGACTTGATAATCAATTATGTTTTATGAGATGATCAGTGCTAAAACTATTATTTTGTTTCAAGGGGTATTCTGTATGGGAGGTGCAGAAGATTGGAATTATA
GTGTATATATATGTGTTGTTTGTCATGGTTGTGAGAATTCAACAAGGCATGTTGAGAAAAGTTTTTATTTTTATTTTTTTACTGTAAATGATTTTAGAGACAATGTTATTGCCAAGAGAAACAACATTCAATTCATTAGCGATCCCAAAGTTTATTGCAC