NA ----> NA

Intron 1 GTATGTGTTT ... TGATATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	54.58	57	53	84
A2	61.9	28	35	56
A3	56.67	39	51	51
A4	57.89	28	24	53
A5	60.68	23	28	43
A6	42.22	39	19	38
A7	49.16	91	61	115
A8	47.85	79	47	98
A9	54.24	54	37	91
A10	46.13	87	32	117

>TRINITY_DN122610_c1_g2_i1 from sample A3 / Example spliced isoform

CTGTTGAATATTTTTGTGGAGAAGGGACAGAGTAGGAGTCCTCTTTTGCTGCAAAATTCGTGGAAATTAAGGCAGTCCCAAAGAGCCAGCTGATGCAGATTCTACCGATGCAGTGGGCACAGGTAAAGGTGAAAGAAAACGTATGTTGATGCAGATTCTATCGCAGTGGGTACAGGTGAAGGTGAAAGAAAACTCATAGG
TCTTCCTTCTGATTTGGTAAGGCAGCGCTGGCATTATCCGTTCGACTCCGACTATTCCCATGCTTTTTGGATCATGGCCGGGTCTCACTCGACCAGGGTAAGATGTCGGTAGGTGCCTGTGGATATATGCAGCTTTATGCAGGGAATCATATAAGCACCGAAGGGCCGACGAACTACTAGACACGAACAGCCGGTAAACG
CGAACTCAACTCAGGGTGCTTTAGATTGGTAGTAACCACCAATGACAGGACACCGATAGGGGAGAAGGACGGCAAGAGCGATGCTGTTCACACTTCTCATATGCTAGCCTGGGCTTCTTCGTGGTTTTCATTTAAAATTGGGTGGGGAAACTAAGGCAGTCCCAAACAGTCCAGGTGATGTAGATTCCACAGGTGCAGTG
GGTTCAAGCAAAGGTTAGTGAGAAGAAACATTGGTACACAAATCT

>TRINITY_DN122610_c1_g2_i16 from sample A3 / Example unspliced isoform:

CTGTTGAATATTTTTGTGGAGAAGGGACAGAGTAGGAGTCCTCTTTTGCTGCAAAATTCGTGGAAATTAAGGCAGTCCCAAAGAGCCAGCTGATGCAGATTCTACCGATGCAGTGGGCACAGGTAAAGGTGAAAGAAAACGTATGTTGATGCAGATTCTATCGCAGTGGGTACAGGTGAAGGTGAAAGAAAACgtatgtg
tttttaattcaactaattttttgcaaatatttatataatgtgtttttaaaaatttgaggcaaagcagataaagcgaagatggaggagctgtttgaatctgtgtgtgacattcaaacacaattgatatgtagTCATAGGTCTTCCTTCTGATTTGATAAGGCAGCGCTGGCATTATCCGTTCGACTCCGACTATTCCCATG
CTTTTTGGATCATGGCCGGGTCTCACTCGACCAGGGTAAGATGTCGGTAGGTGCCTGTGGATATATGCAGCTTTATGCAGGGAATCATATAAGCACCGAAGGGCCGACGAACTACTAGACACGAACAGCCGGTAAACGCGAACTCAACTCAGGGTGCTTTAGATTGGTAGTAACCACCAATGACAGGACACCGATAGGGG
AGAAGGACGGCAAGAGCGATGCTGTTCACACTTCTCATATGCTAGCCTGGGCTTCTTCGTGGTTTTCATTTAAAATTGGGTGGGGAAACTAAGGCAGTCCCAAACAGTCCAGGTGATGTAGATTCCACAGGTGCAGTGGGTTCAAGCAAAGGTTAGTGAGAAGAAACATTGGTACACAAATCT

Intron 2 GTAAAGGTGA ... GTGGGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.03	60	40	47
A2	40.69	43	19	40
A3	47.02	40	32	39
A4	37.18	49	14	44
A5	33.33	32	15	17
A6	41.05	28	18	21
A7	45.04	97	74	85
A8	46.38	74	59	69
A9	43.93	67	41	64
A10	36.32	128	64	82

>TRINITY_DN45493_c0_g3_i3 from sample A6 / Example spliced isoform

TTCTTCTTTCATTGATTGGAGATAATACAGAATCGCTGGAAAACAAGGGCGGGGCCGCGGGCTGGGTAGGAGAGCAGCGCCGTTCATTCATTAATTGAGGGCTGATGGGATGGTGTCCTTGGCTGGACGCAGAGCAGAGCTTGAATTCTGCTTTATTTTTGCTTTTGCTTTTATTGTTGTGTTTATGATGCTTTGATTTT
TGTGTTTCTGGTACTTGATTGTTATTTTTATTTTCCATTTCGAGCATGGCCATGCTATTTTGGCTGCTGTCTTGTCTCTTTTCTATTCATTTTTCATTTCTTCATAGTTTTTTCCTTTTTAAGTTTAATGGGTGGCCGTGGGATAATAAAGCAGTCTCAAACTGCCAAGGCGATGTCGATTTCAGGTGCATTCAGCGCAT
GTCAAGGTTAAAAAAGTAAAGGTAGAGAAGCTGTTGAATATTTTTGTGGAGAAGGGACAGAGTAGGAGTCCTCTCTTGCTGCAAAATTCGTGGAAATTAAGGCAGTCCAAAAGAGCCAGCTGATGCAGATTCTACCGATGCAGTGGGCACAGATAAAGCGAAGATGGAGGAGCTGTTTGAATCTGTGTGTGACATTCAAA
CACAATTGATATGTAGTCATAGGTCTTCCTTCTGATTTGGTAAGGCAGCGCTGGCATTATCCGTTCGACTCCGACTATTCCCATGCTTTTTGGATCATGGCCGGGTCTCACTCGACCAGGGTAAGATGTCGGTAGGTGCCTGTGGATATATGCAGCTTTATGCAGGGAA

>TRINITY_DN45493_c0_g3_i6 from sample A6 / Example unspliced isoform:

TTCTTCTTTCATTGATTGGAGATAATACAGAATCGCTGGAAAACAAGGGCGGGGCCGCGGGCTGGGTAGGAGAGCAGCGCCGTTCATTCATTAATTGAGGGCTGATGGGATGGTGTCCTTGGCTGGACGCAGAGCAGAGCTTGAATTCTGCTTTATTTTTGCTTTTGCTTTTATTGTTGTGTTTATGATGCTTTGATTTT
TGTGTTTCTGGTACTTGATTGTTATTTTTATTTTCCATTTCGAGCATGGCCATGCTATTTTGGCTGCTGTCTTGTCTCTTTTCTATTCATTTTTCATTTCTTCATAGTTTTTTCCTTTTTAAGTTTAATGGGTGGCCGTGGGATAATAAAGCAGTCTCAAACTGCCAAGGCGATGTCGATTTCAGGTGCATTCAGCGCAT
GTCAAGGTTAAAAAAGGAAATTAAGGCAGTCCAAAAGAGCCAGCTGATGCAGATTCTACCGATGCAGTGGGCACAGgtaaaggtgaaagaaaacgtatgttgatgcagattctatcgcagtgggtacagATAAAGCGAAGATGGAGGAGCTGTTTGAATCTGTGTGTGACATTCAAACACAATTGATATGTAGTCATAGG
TCTTCCTTCTGATTTGGTAAGGCAGCGCTGGCATTATCCGTTCGACTCCGACTATTCCCATGCTTTTTGGATCATGGCCGGGTCTCACTCGACCAGGGTAAGATGTCGGTAGGTGCCTGTGGATATATGCAGCTTTATGCAGGGAA