NA ----> NA

Intron 1 GTAAGTTCCG ... TTCGTAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.72	43	169	26
A2	76.92	48	160	16
A3	77.33	50	170.5	12
A4	82.64	39.5	188	6
A5	82.51	19.5	92	2
A6	70.66	114	274.5	127
A7	72.45	143	376	136
A8	69.39	67.5	153	154
A9	75.16	136.5	413	158
A10	78.17	100	358	58

>TRINITY_DN46479_c2_g3_i4 from sample A7 / Example spliced isoform

CTGAAATATTACCCAAACCCAAGATACCAATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTATCATCATTCATATTCGCAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCTCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGTAGAGA
AGGGTTCTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGAGAAACGCACCAACAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCCTTGATGAAGACTATCTACACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATTAGTGAA
TCTGTTCTTTTTTTTTATATATGTATGTGTATATTTACTGTTTTTTTTTTTT

>TRINITY_DN46479_c2_g3_i2 from sample A7 / Example unspliced isoform:

CTGAAATATTACCCAAACCCAAGATACCAATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTATCATCATTCATATTCGCAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCTCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGTAGAGA
AGGGTTCTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGgtaagttccggacttcatctttgcatatgttagtttgtgcttcacttagtttccatggataatgcaacgaataaaaacgatgaaccccaagaacccggtcgagtaagcaaagaaacagttgatttagcggtgaaactgctcctgaaaatgattagt
tcaaagcgtagtttaatatctttggtcgttattctgaagtcgagatctgacagtacagcaatgattcccagaacatcccagtacattccagtgcctcatcccctgtattctattgatggaagccaagagatctgtttgattattggtaataaaaaaaagggtttaaatgccgagttagccgagaacaagattaaggagga
gggtttacctatttcaaaggtctttcaattatccacgctcacggctgattataggaagtgtaagggctctaaaaagccttccctgtcgtttgatctgtttatggtctgcaaatctttattttccaaggcctctgggttttattggtcaataagaaatcatatgatccctgtggatttgacccacgagcgatggaagggac
agatggagttagcttgcagtttgacttctatctccatttgtcattcttctcgtgttgttaaagtaggtagggtttctcaggcaagtaaggaaattgtagagaatattattgccgtgatagatcgtcttgtgtccgagattccggaaaagtggaataacataagttcaatgcatctgcagtcctcaggccctttgcaatgc
ccactttaccagtcctttcctgtaccagaaacagaatctgaaaccgatgaaccagggatgaatgggaaaggaggaatttcgagtaagaaaaggactaaacatgcagaaaaaaccctggatgcaaagaggaagaaaggcttagcttcagagggcaaggcgggctcttttaatggtctagcggctggtgatggagttccaaa
ggatgaacctcctgcagcttcaggactcgaaaaggaaaacgggtctgtagattcgcaaggaggggtggtggccaatagtgtcgaggtcacagcaaagaagaaaaaattgagcagatagaaaaaaagctctgttagagatgtatgagtctgcagcttcaagctgcttttgttttggtactaacagatgcttttgtcaaaga
aaatgacggtgcctgggccatgcagtttctgcacctgctactggaattggatgcctgtcaatgctcgacagcggttaactgctttgggtttgagatgggttttaaacaaaaatgtttagcatctgacccttattatttttttctttgggcttgattacaatttactgtataagttgatgattacaatttactgtataagt
tgatgcagctaatcgtctacttcatattgtaacggtggatatttgtcaaactatttcctctttgtgcttatataagttatattgaatagatttataattgctgcttttattcgtaatagAGAAACGCACCAACAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCCTTGATGAAGACT
ATCTACACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATTAGTGAATCTGTTCTTTTTTTTTATATATGTATGTGTATATTTACTGTTTTTTTTTTTT

Intron 2 GTAAGTTCCG ... GCATCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.31	65.2	169	207.8
A2	72.18	72.8	160	217.2
A3	69.65	78.5	170.6	189
A4	71.02	73	188	169.8
A5	70.16	44.2	92	115.5
A6	65.68	236.3	274.6	629.5
A7	70.09	255	376	818.9
A8	73.41	166.2	153	763
A9	67.54	273.6	413	725.8
A10	65.37	238.9	358	543.8

>TRINITY_DN123803_c0_g1_i1 from sample A1 / Example spliced isoform

TATTACCCAAACCCAAGATACCGATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTTTCATCATTCATATTCACAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCGCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGTAGAGAAGGGTT
CTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGTCCTCAGGCCCTTTGCAATGCCCACTTTACCAGTCCTTTCCTGTACCAGAAACAGAATCTGAAACCGATGAACCAGGGATGAATGGGAAAGGAGGAATTTCGAGTAAGAAAAGGACTAAACATGCAGAAAAAACCCTGGATGCAAAGAGGAAGAAAGGCTTA
GCTTCAGAGGGCAAGGCGGGCTCTTTTAATGGTCTAGCGGCTGGTGATGGAGTTCCAAAGGATGAACCTCCTGCAGCTTCAGGACTCGAAAAGGAAAACGGGTCTGTAGATTCGCAAGGAGGGGTGGTGGCCAATAGTGTCGAGGTCACAGCAAAGAAGAAAAAATTGAGCAGATAGAAAAAAAGCTCTGTTAGAGATGT
ATGAGTCTGCAGCTTCAAGCTGCTTTTGTTTTGGTACTAACAGATGCTTTTGTCAAAGAAAATGACGGTGCCTGGGCCATGCAGTTTCTGCACCTGCTACTGGAATTGGATGCCTGTCAATGCTCGACAGCGGTTAACTGCTTTGGGTTTGAGATGGGTTTTAAACAAAAATGTTTAGCATCTGACCCTTATTATTTTTT
TCTTTGGGCTTGATTACAATTTACTGTATAAGTTGATGATTACAATTTACTGTATAAGTTGATGCAGCTAATCGTCTACTTCATATTGTAACGGTGGATATTTGTCAAACTATTTCCTCTTTGTGCTTATATAAGTTATATTGAATAGATTTATAATTGCTGCTTTTATTCGTAACAGAGAAACGCACCAACAAAGAAAC
TAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCCTTGATGAAGACTATCTACACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATT

>TRINITY_DN123803_c0_g1_i2 from sample A1 / Example unspliced isoform:

TATTACCCAAACCCAAGATACCGATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTTTCATCATTCATATTCACAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCGCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGTAGAGAAGGGTT
CTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGgtaagttccggacttcatctttgcatatgttagttcgtgcttcacttagtttccatggataatgcaacgaataaaaacgatgaaccccaagaacccggtcgagtaagcaaagaaacagttgatttagcggtgaaactgctcctgaaaatgattagttcaaag
cgtagtttaatatctttggtcgttatcctgaagtcgagatctgacagtacagcaatgattcccagaacatcccagtacattccagtgcctcatcccctgtattctattgatggaagccaagagatctgtttgattattggtaataaaaaaaagggtttaaatgccgagttagccgagaacaagattaaggaggagggttt
acctatttcaaaggtctttcaattatccacgctcacggctgattataggaagtgtaagggctctaaaaagccttccctgtcgtttgatctgtttatggtctgcaaatctttattttccaaggcctctgggttttattggtcaataagaaatcatatgatccctgtggatttgacccacgagcgatggaagggacagatgg
agttagcttgcagtttgacttctatctccatttgtcattcttctcgtgttgttaaagtaggtagggtttctcaggcaagtaaggaaattgtagagaatattattgccgtgatagatcgtcttgtgtccgagattccggaaaagtggaataacataagttcaatgcatctgcagTCCTCAGGCCCTTTGCAATGCCCACTT
TACCAGTCCTTTCCTGTACCAGAAACAGAATCTGAAACCGATGAACCAGGGATGAATGGGAAAGGAGGAATTTCGAGTAAGAAAAGGACTAAACATGCAGAAAAAACCCTGGATGCAAAGAGGAAGAAAGGCTTAGCTTCAGAGGGCAAGGCGGGCTCTTTTAATGGTCTAGCGGCTGGTGATGGAGTTCCAAAGGATGA
ACCTCCTGCAGCTTCAGGACTCGAAAAGGAAAACGGGTCTGTAGATTCGCAAGGAGGGGTGGTGGCCAATAGTGTCGAGGTCACAGCAAAGAAGAAAAAATTGAGCAGATAGAAAAAAAGCTCTGTTAGAGATGTATGAGTCTGCAGCTTCAAGCTGCTTTTGTTTTGGTACTAACAGATGCTTTTGTCAAAGAAAATGA
CGGTGCCTGGGCCATGCAGTTTCTGCACCTGCTACTGGAATTGGATGCCTGTCAATGCTCGACAGCGGTTAACTGCTTTGGGTTTGAGATGGGTTTTAAACAAAAATGTTTAGCATCTGACCCTTATTATTTTTTTCTTTGGGCTTGATTACAATTTACTGTATAAGTTGATGATTACAATTTACTGTATAAGTTGATGC
AGCTAATCGTCTACTTCATATTGTAACGGTGGATATTTGTCAAACTATTTCCTCTTTGTGCTTATATAAGTTATATTGAATAGATTTATAATTGCTGCTTTTATTCGTAACAGAGAAACGCACCAACAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCCTTGATGAAGACTATCTAC
ACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATT

Intron 3 GTAAGTTCCG ... TGATTCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	68.99	72.3	169	148
A2	70.5	67.2	160	153
A3	70.22	81.2	170.5	204
A4	76.18	57.3	188	172
A5	77	27.3	92	86
A6	70.31	126.2	274.5	308
A7	72.14	159.8	376	427
A8	69.7	85.2	153	233
A9	70	185.7	413	432
A10	73.84	140.8	358	419

>TRINITY_DN118734_c0_g2_i5 from sample A3 / Example spliced isoform

CGCCGCTGAAATATTACCCAAACCCAAGATACCGATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTATCATCATTCATATTCGCAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCTCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGT
AGAGAAGGGTTCTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGAACATCCCAGTACATTCCAGTGCCTCATCCCCTGTATTCTATTGATGGAAGCCAAGAGATCTGTTTGATTATTGGTAATAAAAAAAAGGGTTTAAATGCCGAGTTAGCCGAGAACAAGATTAAGGAGGAGGGTTTACCTATTTCAAAGGTC
TTTCAATTATCCACGCTCACGGCTGATTATAGGAAGTGTAAGGGCTCTAAAAAGCCTTCCCTGTCGTTTGATCTGTTTATGGTCTGCAAATCTTTATTTTCCAAGGTCTCTGGGTTTTATTGGTCAATAAAAAATCATATGATCCCTGTGGATTTGACCCACGAGCGATGGAAGGGACAGATGGAGTTAGCTTGCAGTTT
GACTTCTATCTCCATTTGTCATTCTTCTCGTGTTGTTAAAGTAGGTAGGGTTTCTCAGGCAAGTAAGGAAATTGTAGAGAATATTATTGCCGTGATAGATCGTCTTGTGTCCGAGATTCCGGAAAAGTGGAATAACATAAGTTCAATGCATCTGCAGTCCTCAGGCCCTTTGCAATGCCCACTTTACCAGTCCTTTCCTG
TACCAGAAACCGATGAACCAGGGATGAATGGGAAAGGAGGAATTACAGGAGGAATTTCGAGTAAGAAAAGGACTAAACATGCAGAAAAAACCCTGGATGCAAAGAGGAAGAAAGGCTTAGCTTCAGAGGGCAAGGCGGGCTCTTTTAATGGTCTAGCGGTCGGTGATGGAGTTCCGAAGGATGAACCTCCTGCAGCTTCA
GGACTCGAAAAGGAAAACGAGTCTGTAGATTCGCAAGGAGGGGTGGCGGCTAGTGTCGAGGTCACAGCAAAGAAAAAAAATTGAGCAGATAGAAAAAAAGCTCTGTTAGAGATGTATGAGTCTGCAGCTTCAAGCTGCTTTTGTTTTGGTACTAACAGATGCTTTTGTCAAAGAAAATGACGGTGCCTGGGCCATGCAGT
TTCTGCACCTGCTACTGGAATTGGATGCCTGTCAATGCTTGACAGCGGTTAACTGCTTTGGGTTTGAGATGGGTTTTAAACAAAATGTTTAGCATCTGACCCTTATTATTTTTTTCTTTATTATTTTTTTCTTTGGGCTTGATTACAATTTACTGTATAAGTTGATGATTACAATTTACTGTATAAGTTGATGCAGCTAA
TCGTCTACTTCATATTGTAACGGTGGATATTTGTCAAACTATTTCCTCTTTGTGCTTATATAAGTTATATTGAATAGATTTATAATTGCTGCTTTTATTCGTAATAGAGAAACGCACCAAAAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCCTTGATGAAGACTATCTACACCTCC
CTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATTAGTGAATCTGT

>TRINITY_DN118734_c0_g2_i1 from sample A3 / Example unspliced isoform:

CGCCGCTGAAATATTACCCAAACCCAAGATACCGATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTATCATCATTCATATTCGCAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCTCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGT
AGAGAAGGGTTCTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGgtaagttccggacttcatctttgcatatgttagttcgtgcttcacttagtttccatggataatgcaacgaataaaaacgatgaaccccaagaacccggtcgagtaagcaaagaaacagttgatttagcggtgaaactgctcctgaaaatga
ttagttcaaagcgtagtttaatatctttggtcgttatcctgaagtcgagatctgacagtacagcaatgattcccagAACATCCCAGTACATTCCAGTGCCTCATCCCCTGTATTCTATTGATGGAAGCCAAGAGATCTGTTTGATTATTGGTAATAAAAAAAAGGGTTTAAATGCCGAGTTAGCCGAGAACAAGATTAAG
GAGGAGGGTTTACCTATTTCAAAGGTCTTTCAATTATCCACGCTCACGGCTGATTATAGGAAGTGTAAGGGCTCTAAAAAGCCTTCCCTGTCGTTTGATCTGTTTATGGTCTGCAAATCTTTATTTTCCAAGGTCTCTGGGTTTTATTGGTCAATAAAAAATCATATGATCCCTGTGGATTTGACCCACGAGCGATGGAA
GGGACAGATGGAGTTAGCTTGCAGTTTGACTTCTATCTCCATTTGTCATTCTTCTCGTGTTGTTAAAGTAGGTAGGGTTTCTCAGGCAAGTAAGGAAATTGTAGAGAATATTATTGCCGTGATAGATCGTCTTGTGTCCGAGATTCCGGAAAAGTGGAATAACATAAGTTCAATGCATCTGCAGTCCTCAGGCCCTTTGC
AATGCCCACTTTACCAGTCCTTTCCTGTACCAGAAACCGATGAACCAGGGATGAATGGGAAAGGAGGAATTACAGGAGGAATTTCGAGTAAGAAAAGGACTAAACATGCAGAAAAAACCCTGGATGCAAAGAGGAAGAAAGGCTTAGCTTCAGAGGGCAAGGCGGGCTCTTTTAATGGTCTAGCGGTCGGTGATGGAGTT
CCGAAGGATGAACCTCCTGCAGCTTCAGGACTCGAAAAGGAAAACGAGTCTGTAGATTCGCAAGGAGGGGTGGCGGCTAGTGTCGAGGTCACAGCAAAGAAAAAAAATTGAGCAGATAGAAAAAAAGCTCTGTTAGAGATGTATGAGTCTGCAGCTTCAAGCTGCTTTTGTTTTGGTACTAACAGATGCTTTTGTCAAAG
AAAATGACGGTGCCTGGGCCATGCAGTTTCTGCACCTGCTACTGGAATTGGATGCCTGTCAATGCTTGACAGCGGTTAACTGCTTTGGGTTTGAGATGGGTTTTAAACAAAATGTTTAGCATCTGACCCTTATTATTTTTTTCTTTATTATTTTTTTCTTTGGGCTTGATTACAATTTACTGTATAAGTTGATGATTACA
ATTTACTGTATAAGTTGATGCAGCTAATCGTCTACTTCATATTGTAACGGTGGATATTTGTCAAACTATTTCCTCTTTGTGCTTATATAAGTTATATTGAATAGATTTATAATTGCTGCTTTTATTCGTAATAGAGAAACGCACCAAAAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAG
GCCTTGATGAAGACTATCTACACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATTAGTGAATCTGT

Intron 4 GTAAGTTCCG ... TTCGTAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.72	43	169	26
A2	76.92	48	160	16
A3	77.33	50	170.5	12
A4	82.64	39.5	188	6
A5	82.51	19.5	92	2
A6	70.66	114	274.5	127
A7	72.45	143	376	136
A8	69.39	67.5	153	154
A9	75.16	136.5	413	158
A10	78.17	100	358	58

>TRINITY_DN46479_c2_g3_i4 from sample A7 / Example spliced isoform

CTGAAATATTACCCAAACCCAAGATACCAATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTATCATCATTCATATTCGCAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCTCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGTAGAGA
AGGGTTCTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGAGAAACGCACCAACAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCCTTGATGAAGACTATCTACACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATTAGTGAA
TCTGTTCTTTTTTTTTATATATGTATGTGTATATTTACTGTTTTTTTTTTTT

>TRINITY_DN46479_c2_g3_i1 from sample A7 / Example unspliced isoform:

CTGAAATATTACCCAAACCCAAGATACCAATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTATCATCATTCATATTCGCAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCTCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGTAGAGA
AGGGTTCTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGgtaagttccggacttcatctttgcatatgttagtttgtgcttcacttagtttccatggataatgcaacgaataaaaacgatgaaccccaagaacccggtcgagtaagcaaagaaacagttgatttagcggtgaaactgctcctgaaaatgattagt
tcaaagcgtagtttaatatctttggtcgttattctgaagtcgagatctgacagtacagcaatgattcccagaacatcccagtacattccagtgcctcatcccctgtattctattgatggaagccaagagatctgtttgattattggtaataaaaaaaagggtttaaatgccgagttagccgagaacaagattaaggagga
gggtttacctatttcaaaggtctttcaattatccacgctcacggctgattataggaagtgtaagggctctaaaaagccttccctgtcgtttgatctgtttatggtctgcaaatctttattttccaaggcctctgggttttattggtcaataagaaatcatatgatccctgtggatttgacccacgagcgatggaagggac
agatggagttagcttgcagtttgacttctatctccatttgtcattcttctcgtgttgttaaagtaggtagggtttctcaggcaagtaaggaaattgtagagaatattattgccgtgatagatcgtcttgtgtccgagattccggaaaagtggaataacataagttcaatgcatctgcagtcctcaggccctttgcaatgc
ccactttaccagtcctttcctgtaccagaaaccgatgaaccagggatgaatgggaaaggaggaattacaggaggaatttcgagtaagaaaaggactaaacatgcagaaaaaaccctggatgcaaagaggaagaaaggcttagcttcagagggcaaggcgggctcttttaatggtctagcggtcggtgatggagttccgaa
ggatgaacctcctgcagcttcaggactcgaaaaggaaaacgggtctgtagattcgcaaggaggggtggcggctagtgtcgaggtcacagcaaagaaaaaaaattgagcagatagaaaaaaagctctgttagagatgtatgagtctgcagcttcaagctgcttttgttttggtactaacagatgcttttgtcaaagaaaat
gacggtgcctgggccatgcagtttctgcacctgctactggaattggatgcctgtcaatgctcgacagcggttaactgctttgggtttgagatgggttttaaacaaaatgtttagcatctgacccttattatttttttctttattatttttttctttgggcttgattacaatttactgtataagttgatgattacaattta
ctgtataagttgatgcagctaatcgtctacttcatattgtaacggtggatatttgtcaaactatttcctctttgtgcttatataagttatattgaatagatttataattgctgcttttattcgtaatagAGAAACGCACCAACAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCCTT
GATGAAGACTATCTACACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATTAGTGAATCTGTTCTTTTTTTTTATATATGTATGTGTATATTTACTGTTTTTTTTTTTT

Intron 5 GTTTAGCATC ... TTCGTAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.46	10	47	26
A2	94.44	3	51	16
A3	100	0	69	12
A4	100	0	44	6
A5	97.37	1	37	2
A6	96.75	24	714	127
A7	95.36	27	555	136
A8	96.7	26	761	154
A9	95.61	27	588	158
A10	96.95	11	350	58

>TRINITY_DN46479_c2_g3_i5 from sample A7 / Example spliced isoform

GTTAGAGATGGGTTTTAAACAAAATAGAAACGCACCAACAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCCTTGATGAAGACTATCTACACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATTAGTGAATCTGTTCTTTTTTTTTATA
TATGTATGTGTATATTTACTGTTTTTTTTTTTT

>TRINITY_DN46479_c2_g3_i1 from sample A7 / Example unspliced isoform:

CTGAAATATTACCCAAACCCAAGATACCAATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTATCATCATTCATATTCGCAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCTCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGTAGAGA
AGGGTTCTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGGTAAGTTCCGGACTTCATCTTTGCATATGTTAGTTTGTGCTTCACTTAGTTTCCATGGATAATGCAACGAATAAAAACGATGAACCCCAAGAACCCGGTCGAGTAAGCAAAGAAACAGTTGATTTAGCGGTGAAACTGCTCCTGAAAATGATTAGT
TCAAAGCGTAGTTTAATATCTTTGGTCGTTATTCTGAAGTCGAGATCTGACAGTACAGCAATGATTCCCAGAACATCCCAGTACATTCCAGTGCCTCATCCCCTGTATTCTATTGATGGAAGCCAAGAGATCTGTTTGATTATTGGTAATAAAAAAAAGGGTTTAAATGCCGAGTTAGCCGAGAACAAGATTAAGGAGGA
GGGTTTACCTATTTCAAAGGTCTTTCAATTATCCACGCTCACGGCTGATTATAGGAAGTGTAAGGGCTCTAAAAAGCCTTCCCTGTCGTTTGATCTGTTTATGGTCTGCAAATCTTTATTTTCCAAGGCCTCTGGGTTTTATTGGTCAATAAGAAATCATATGATCCCTGTGGATTTGACCCACGAGCGATGGAAGGGAC
AGATGGAGTTAGCTTGCAGTTTGACTTCTATCTCCATTTGTCATTCTTCTCGTGTTGTTAAAGTAGGTAGGGTTTCTCAGGCAAGTAAGGAAATTGTAGAGAATATTATTGCCGTGATAGATCGTCTTGTGTCCGAGATTCCGGAAAAGTGGAATAACATAAGTTCAATGCATCTGCAGTCCTCAGGCCCTTTGCAATGC
CCACTTTACCAGTCCTTTCCTGTACCAGAAACCGATGAACCAGGGATGAATGGGAAAGGAGGAATTACAGGAGGAATTTCGAGTAAGAAAAGGACTAAACATGCAGAAAAAACCCTGGATGCAAAGAGGAAGAAAGGCTTAGCTTCAGAGGGCAAGGCGGGCTCTTTTAATGGTCTAGCGGTCGGTGATGGAGTTCCGAA
GGATGAACCTCCTGCAGCTTCAGGACTCGAAAAGGAAAACGGGTCTGTAGATTCGCAAGGAGGGGTGGCGGCTAGTGTCGAGGTCACAGCAAAGAAAAAAAATTGAGCAGATAGAAAAAAAGCTCTGTTAGAGATGTATGAGTCTGCAGCTTCAAGCTGCTTTTGTTTTGGTACTAACAGATGCTTTTGTCAAAGAAAAT
GACGGTGCCTGGGCCATGCAGTTTCTGCACCTGCTACTGGAATTGGATGCCTGTCAATGCTCGACAGCGGTTAACTGCTTTGGGTTTGAGATGGGTTTTAAACAAAATgtttagcatctgacccttattatttttttctttattatttttttctttgggcttgattacaatttactgtataagttgatgattacaattta
ctgtataagttgatgcagctaatcgtctacttcatattgtaacggtggatatttgtcaaactatttcctctttgtgcttatataagttatattgaatagatttataattgctgcttttattcgtaatagAGAAACGCACCAACAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCCTT
GATGAAGACTATCTACACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATTAGTGAATCTGTTCTTTTTTTTTATATATGTATGTGTATATTTACTGTTTTTTTTTTTT

Intron 6 GTAAGGGCTC ... GCATCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	93.46	12	135	208
A2	90.48	18	126	216
A3	85.71	26	123	189
A4	89.14	17	108	171
A5	85.57	14	50	116
A6	89.63	49	217	630
A7	87.77	82	356	821
A8	93.6	34	231	763
A9	89.24	63	319	726
A10	86.75	64	292	546

>TRINITY_DN44291_c0_g2_i6 from sample A9 / Example spliced isoform

CTGAAATATTACCCAAACCCAAGATACCGATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTTTCATCATTCATATTCACAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCGCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGTAGAGA
AGGGTTCTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGAACATCCCAGTACATTCCAGTGCCTCATCCCCTGTATTCTATTGATGGAAGCCAAGAGATCTGTTTGATTATTGGTAATAAAAAAAAGGGTTTAAATGCCGAGTTAGCCGAGAACAAGATTAAGGAGGAGGGTTTACCTATTTCAAAGGTCTTTCA
ATTATCCACGCTCACGGCTGATTATAGGAAGTTCCTCAGGCCCTTTGCAATGCCCACTTTACCAGTCCTTTCCTGTACCAGAAACAGAATCTGAAACCGATGAACCAGGGATGAATGGGAAAGGAGGAATT

>TRINITY_DN44291_c0_g2_i4 from sample A9 / Example unspliced isoform:

CTGAAATATTACCCAAACCCAAGATACCGATTCATGGTTAAACCCTACCTACTCAAAACATACACATTACAAAACAAAGTTTAGGGTTTAAGTTGTGCAGGGTTTCATCATTCATATTCACAGTTTCTTAGTTTTGGCAATTGATGTACAGCATTCATCGCAACAGTTTCTCAGTTTTGCCAAGGGATTAAACGTAGAGA
AGGGTTCTTCTTCATTTCCACCGGCGTTCTGAGGAGCATTGCTGGTAAGTTCCGGACTTCATCTTTGCATATGTTAGTTCGTGCTTCACTTAGTTTCCATGGATAATGCAACGAATAAAAACGATGAACCCCAAGAACCCGGTCGAGTAAGCAAAGAAACAGTTGATTTAGCGGTGAAACTGCTCCTGAAAATGATTAGT
TCAAAGCGTAGTTTAATATCTTTGGTCGTTATCCTGAAGTCGAGATCTGACAGTACAGCAATGATTCCCAGAACATCCCAGTACATTCCAGTGCCTCATCCCCTGTATTCTATTGATGGAAGCCAAGAGATCTGTTTGATTATTGGTAATAAAAAAAAGGGTTTAAATGCCGAGTTAGCCGAGAACAAGATTAAGGAGGA
GGGTTTACCTATTTCAAAGGTCTTTCAATTATCCACGCTCACGGCTGATTATAGGAAGTgtaagggctctaaaaagccttccctgtcgtttgatctgtttatggtctgcaaatctttattttccaaggcctctgggttttattggtcaataagaaatcatatgatccctgtggatttgacccacgagcgatggaagggac
agatggagttagcttgcagtttgacttctatctccatttgtcattcttctcgtgttgttaaagtaggtagggtttctcaggcaagtaaggaaattgtagagaatattattgccgtgatagatcgtcttgtgtccgagattccggaaaagtggaataacataagttcaatgcatctgcagTCCTCAGGCCCTTTGCAATGC
CCACTTTACCAGTCCTTTCCTGTACCAGAAACAGAATCTGAAACCGATGAACCAGGGATGAATGGGAAAGGAGGAATTTCGAGTAAGAAAAGGACTAAACATGCAGAAAAAACCCTGGATGCAAAGAGGAAGAAAGGCTTAGCTTCAGAGGGCAAGGCGGGCTCTTTTAATGGTCTAGCGGCTGGTGATGGAGTTCCAAA
GGATGAACCTCCTGCAGCTTCAGGACTCGAAAAGGAAAACGAGTCTGTAGATTCGCAAGGAGGGGTGGTGGCCAATAGTGTCGAGGTCACAGCAAAGAAGAAAAAATTGAGCAGATAGAAAAAAAGCTCTGTTAGAGATGTATGAGTCTGCAGCTTCAAGCTGCTTTTGTTTTGGTACTAACAGATGCTTTTGTCAAAGA
AAATGACGGTGCCTGGGCCATGCAGTTTCTGCACCTGCTACTGGAATTGGATGCCTGTCAATGCTCGACAGCGGTTAACTGCTTTGGGTTTGAGATGGGTTTTAAACAAAAATGTTTAGCATCTGACCCTTATTATTTTTTTCTTTGGGCTTGATTACAATTTACTGTATAAGTTGATGATTACAATTTACTGTATAAGT
TGATGCAGCTAATCGTCTACTTCATATTGTAACGGTGGATATTTGTCAAACTATTTCCTCTTTGTGCTTATATAAGTTATATTGAATAGATTTATAATTGCTGCTTTTATTCGTAATAGAGAAACGCACCAACAAAGAAACTAAGGTGGCTTTAGCACATGATTTCACTACTCCTCTAGTTGAAGGCATTGATGAAGACT
ATCTACACCTCCCTCTTGAACATTTATTTTGATAAAAAATCTACAGCGTTTGAAGCACTTATTTGTATTAGTGAATCTGTTCTTTTTTTTATATATGTATGTGTATATTTACTGTTTTTTTTTTTCATTGGAATATGTCATGGGGAAATCCTCAAGTATTTCCCCTCATTTTGAGGATGGC