NA ----> NA

Intron 1 GTATGTATTT ... GTGATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.49	4	26	7
A2	28.57	5	3	1
A3	42.86	6	6	3
A4	88.89	1	9	7
A5	0	2	0	0
A6	25	9	5	1
A7	22.86	27	12	4
A8	39.78	28	24	13
A9	10.34	26	4	2
A10	9.09	15	2	1

>TRINITY_DN44478_c0_g2_i5 from sample A6 / Example spliced isoform

TGAGTTGCTACAAGAAGGGCATGAGGGGCAAGAGGCTCATGCAGCAGAACCTTCTAAGTTACAAGATGTTGTCATACCTTCAGCGACAACATCTCACGCTGAGTTGCTACAAGAAGGGCATGAGGGGCAAGAGGCTCATGCAGCAGAACCTTCTGAGTTACAAGATGTTGTCATACATTCAGTGACAACATCTCACACTG
AGGTTGTAGATCCCCATCAGAGGGAAATGATTTCATCTAGTGAGCCCCCCTTGGTGGGAATACAACCAGTAGCATTCCCATAGAGAAACAATGGACAGAAGACCATTTCTTAGAATTGATTGAAGTATACAGTACATTGCCACTAGCCTCTATACTTCATAGACTTTCAGTAGTTGGATCTCCTCATAGTCCAAATGTGA
AAAAGGAACAAGTGTATAAGAGAGGCTCCACTCCTAGAAAGGGTGTGGATGTCCTCTATAACAGACACACGAGAGATCCATCTATACCTCACCTAACAAGGGTCATTGATGCTCCAGTTAGTGCCGCATCTGCAATAGGCACACGTCATACAAAGGTAACTATTTCAACAATCACAATGCATAGTATTTAACCATGTTAC
TATATTGTTTATTCATGCATGTATAACTATTTCCCCATTTGGTGATTGCAGCCATTAGCGGCAGAAGTGCCCGGCCCCTCTACTCAATCCATGGTTCTGACTGGGTCTCCTCTTCTAGTGAGATGTTCATTAATGACCAAGTTTTTTTATTCAATTTACCCAAACATGTTGATCAATGTTGAATTGTTGTATTAATTGAG
ATATGATCTTTTATTTGCAAAGCACAACTATAGAGAGGCCTTCCATGATCACACCGCTCACATCTATACCCCCTGAGGATTTGGTGAGTGTTTGTCATAAATAATTTGGCCATAAGTTAATTTTTTAAGTTTGTTAATGGTAGGGTATTAGTACAGTTATTTGATAAAAATAATAAAAAAATTTTGTAGACTCCTGAGAT
GCAGAGTGATCCCAGGGAGATGGTCGATAGAGGAAAGTCAGGGAAAAACAACCAAAAGATTGATTTTTGGTGTTGCATAGTCTTGTATATATGTATTTTGACAT

>TRINITY_DN44478_c0_g2_i10 from sample A6 / Example unspliced isoform:

TGAGTTGCTACAAGAAGGGCATGAGGGGCAAGAGGCTCATGCAGCAGAACCTTCTAAGTTACAAGATGTTGTCATACCTTCAGCGACAACATCTCACGCTGAGTTGCTACAAGAAGGGCATGAGGGGCAAGAGGCTCATGCAGCAGAACCTTCTGAGTTACAAGATGTTGTCATACATTCAGTGACAACATCTCACACTG
AGGTTGTAGATCCCCATCAGAGGGAAATGATTTCATCTAGTGAGCCCCCCTTGGTGGGAATACAACCAGTAGCATTCCCATAGAGAAACAATGGACAGAAGACCATTTCTTAGAATTGATTGAAGTATACAGTACATTGgtatgtatttcatcattttcagtttgttaatacatgctatttcattaaccaatgcatttct
aactacgtcagtattggtgattgtagCCACTAGCCTCTATACTTCATA

Intron 2 GTGAGATGTT ... TTATTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	63.64	8	12	16
A2	71.43	5	12	13
A3	71.43	3	6	9
A4	36	8	2	7
A5	75	1	2	4
A6	24.14	22	7	7
A7	33.33	18	8	10
A8	46.03	34	25	33
A9	15.79	32	9	3
A10	43.48	13	8	12

>TRINITY_DN112479_c0_g1_i3 from sample A1 / Example spliced isoform

TTAATACATGCTATTTCATTAACCAATGCATTTCTAACTACGTCAGTATTGGTGATTGTAGCCACCAGCCTCTATACTTATGAGACTGTTAGTAGTTGGATCTCCTCGTAGTCCGAATGTGCAAAAGGGACAAGTGTATAAGAGAGGCTCCACTCCTAGAAAGGGTGTGGATGTCCTCTATAACAGACACACGAGAGATC
CATCTATACCTCACCTAACAAGGGTCATTGATGCTCCAGTTAGTGCCGCATCTGCAATAGGCACACGTCATACAAAGCCATTAGCGGCAGAAGTGCCCGGCCCCTCTACTCAATCCATGGTTCTGACTGGGTCTCCTCTTCTAAGCACAACTATAGAGAGGCCTTCCATGAGCACACTACTCACAACTATACCCCATGAG
GATTTGACTCGTGAGATGCAGAGCGATCCCAGGGAGATGGTCGATAGAGGAAAGTCATGGAAAAACAGGCGAAAGATTGATTTTTGATGTTGCATAGTCTTGTATATATGTATTTTGACATGTACATGTACACATTGTTGTACTTAGGGATACATGTACATTGACAGGCCATTTGAGGGCTGATTGCTTTGCCATATTTT
GTATTACAAACATTTGACTATCAATGTGTGAT

>TRINITY_DN112479_c0_g1_i5 from sample A1 / Example unspliced isoform:

TTAATACATGCTATTTCATTAACCAATGCATTTCTAACTACGTCAGTATTGGTGATTGTAGCCACCAGCCTCTATACTTATGAGACTGTTAGTAGTTGGATCTCCTCGTAGTCCGAATGTGCAAAAGGGACAAGTGTATAAGAGAGGCTCCACTCCTAGAAAGGGTGTGGATGTCCTCTATAACAGACACACGAGAGATC
CATCTATACCTCACCTAACAAGGGTCATTGATGCTCCAGTTAGTGCCGCATCTGCAATAGGCACACGTCATACAAAGCCATTAGCGGCAGAAGTGCCCGGCCCCTCTACTCAATCCATGGTTCTGACTGGGTCTCCTCTTCTAgtgagatgttcattaatgaccaagtttttttattcaatttatccaaacatgtttatc
aatgttgaattgttgaattaattgagatatgatcttttatttgcagAGCACAACTATAGAGAGGCCTTCCATGAGCACACTACTCACAACTATACCCCATGAGGATTTGGTAAGTGTTTGTCATAAATAATTTGGCCATAAGTTAATTTTTTAATTTTGTTAATGGTAGGGTATTAGTGCAGTTATTTGATAAAAATAAT
GATTTTTTTTGCAGACTCGTGAGATGCAGAGCGATCCCAGGGAGATGGTCGATAGAGGAAAGTCATGGAAAAACAGGCGAAAGATTGATTTTTGATGTTGCATAGTCTTGTATATATGTATTTTGACATGTACATGTACACATTGTTGTACTTAGGGATACATGTACATTGACAGGCCATTTGAGGGCTGATTGCTTTGC
CATATTTTGTATTACAAACATTTGACTATCAATGTGTGAT

Intron 3 GTAAGTGTTT ... TTTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.58	7	9	10
A2	54.84	7	7	10
A3	79.31	3	9	14
A4	50	7	6	8
A5	77.78	1	2	5
A6	23.94	27	6	11
A7	43.4	30	12	34
A8	41.24	52	27	46
A9	17.5	33	6	8
A10	17.65	21	2	7

>TRINITY_DN112479_c0_g1_i3 from sample A1 / Example spliced isoform

TTAATACATGCTATTTCATTAACCAATGCATTTCTAACTACGTCAGTATTGGTGATTGTAGCCACCAGCCTCTATACTTATGAGACTGTTAGTAGTTGGATCTCCTCGTAGTCCGAATGTGCAAAAGGGACAAGTGTATAAGAGAGGCTCCACTCCTAGAAAGGGTGTGGATGTCCTCTATAACAGACACACGAGAGATC
CATCTATACCTCACCTAACAAGGGTCATTGATGCTCCAGTTAGTGCCGCATCTGCAATAGGCACACGTCATACAAAGCCATTAGCGGCAGAAGTGCCCGGCCCCTCTACTCAATCCATGGTTCTGACTGGGTCTCCTCTTCTAAGCACAACTATAGAGAGGCCTTCCATGAGCACACTACTCACAACTATACCCCATGAG

GATTTGACTCGTGAGATGCAGAGCGATCCCAGGGAGATGGTCGATAGAGGAAAGTCATGGAAAAACAGGCGAAAGATTGATTTTTGATGTTGCATAGTCTTGTATATATGTATTTTGACATGTACATGTACACATTGTTGTACTTAGGGATACATGTACATTGACAGGCCATTTGAGGGCTGATTGCTTTGCCATATTTT
GTATTACAAACATTTGACTATCAATGTGTGAT

>TRINITY_DN112479_c0_g1_i5 from sample A1 / Example unspliced isoform:

TTAATACATGCTATTTCATTAACCAATGCATTTCTAACTACGTCAGTATTGGTGATTGTAGCCACCAGCCTCTATACTTATGAGACTGTTAGTAGTTGGATCTCCTCGTAGTCCGAATGTGCAAAAGGGACAAGTGTATAAGAGAGGCTCCACTCCTAGAAAGGGTGTGGATGTCCTCTATAACAGACACACGAGAGATC
CATCTATACCTCACCTAACAAGGGTCATTGATGCTCCAGTTAGTGCCGCATCTGCAATAGGCACACGTCATACAAAGCCATTAGCGGCAGAAGTGCCCGGCCCCTCTACTCAATCCATGGTTCTGACTGGGTCTCCTCTTCTAGTGAGATGTTCATTAATGACCAAGTTTTTTTATTCAATTTATCCAAACATGTTTATC
AATGTTGAATTGTTGAATTAATTGAGATATGATCTTTTATTTGCAGAGCACAACTATAGAGAGGCCTTCCATGAGCACACTACTCACAACTATACCCCATGAGGATTTGgtaagtgtttgtcataaataatttggccataagttaattttttaattttgttaatggtagggtattagtgcagttatttgataaaaataat
gattttttttgcagACTCGTGAGATGCAGAGCGATCCCAGGGAGATGGTCGATAGAGGAAAGTCATGGAAAAACAGGCGAAAGATTGATTTTTGATGTTGCATAGTCTTGTATATATGTATTTTGACATGTACATGTACACATTGTTGTACTTAGGGATACATGTACATTGACAGGCCATTTGAGGGCTGATTGCTTTGC
CATATTTTGTATTACAAACATTTGACTATCAATGTGTGAT

Intron 4 GTAACTATTT ... TATTCATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.65	6	25	2
A2	100	0	18	2
A3	60	8	12	6
A4	72.73	3	8	3
A5	93.75	1	15	8
A6	55.56	8	10	5
A7	52	12	13	3
A8	64.58	17	31	8
A9	50	9	9	1
A10	66.67	4	8	0

>TRINITY_DN44478_c0_g2_i14 from sample A6 / Example spliced isoform

TGAGTTGCTACAAGAAGGGCATGAGGGGCAAGAGGCTCATGCAGCAGAACCTTCTAAGTTACAAGATGTTGTCATACCTTCAGCGACAACATCTCACGCTGAGTTGCTACAAGAAGGGCATGAGGGGCAAGAGGCTCATGCAGCAGAACCTTCTGAGTTACAAGATGTTGTCATACATTCAGTGACAACATCTCACACTG
AGGTTGTAGATCCCCATCAAAGGGAAATGATTTCATTTAGTGAGCTCCCCCCTGGTGGGAATACAACCGATAGCGTGCCCATAGAGATACAACGGGTGGAAGATCGTTTCTTGGATTTGCTTGAAGTATACAGTACATCGCCACCATCCTCTATCCTTATGAGACAATCAATAGTTGGATCTCCTTGTAGTCCAAATGTG
CAAAAGGGACAAGTGTATAAGAGAGGCTCCACTCCTAGAAAGGGTGTGGATGTCCTCTATATTAGAAACACAAGAGATCCATCTACCCCTCACCTAGCAAGGGTCATTGATGCTGCAGTTAGTGTCGCATCTACAATAGCCACAAGTCATACAGAGCTGCAGAAGTTCCCAACCCCTCTTCTCAATCCATGGTTCTGACC
GGGTCTCCTCTTGAGCACAACTATAGAGAGGCCTTCCATGAGCACACTACTCACAACTATACCCCATGAGGATTTGACTCGTGAGATGCAGAGCGATCCCAGGGAGATGGTCGATAGAGGAAAGTCA

>TRINITY_DN44478_c0_g2_i9 from sample A6 / Example unspliced isoform:

TGAGTTGCTACAAGAAGGGCATGAGGGGCAAGAGGCTCATGCAGCAGAACCTTCTAAGTTACAAGATGTTGTCATACCTTCAGCGACAACATCTCACGCTGAGTTGCTACAAGAAGGGCATGAGGGGCAAGAGGCTCATGCAGCAGAACCTTCTGAGTTACAAGATGTTGTCATACATTCAGTGACAACATCTCACACTG
AGGTTGTAGATCCCCATCAAAGGGAAATGATTTCATTTAGTGAGCTCCCCCCTGGTGGGAATACAACCGATAGCGTGCCCATAGAGATACAACGGGTGGAAGATCGTTTCTTGGATTTGCTTGAAGTATACAGTACATCGCCACCATCCTCTATCCTTATGAGACAATCAATAGTTGGATCTCCTTGTAGTCCAAATGTG
CAAAAGGGACAAGTGTATAAGAGAGGCTCCACTCCTAGAAAGGGTGTGGATGTCCTCTATATTAGAAACACAAGAGATCCATCTACCCCTCACCTAGCAAGGGTCATTGATGCTGCAGTTAGTGTCGCATCTACAATAGCCACAAGTCATACAGAGgtaactatttcaacaatcacaatgcatagtatttaaccatgtta
ctatattgtttattcatgcatgtataactatttccccacttggtgattgtattcattagCTGCAGAAGTTCCC