sp|Q3ED78|C3H7_ARATH ----> Zinc finger CCCH domain-containing protein 7

Intron 1 GTAAGCTTCA ... TTTTTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.51	24.8	12.1	14
A2	32.99	16.9	4.8	10
A3	33.91	18.9	7.4	11
A4	21.33	11.2	2.7	3
A5	54.53	5.3	5.2	7
A6	28.83	48.4	13.7	23
A7	25.22	51.9	15.4	19
A8	26.84	63.7	17.8	27
A9	28.79	31.1	17.3	7
A10	41.78	29.1	2.9	32

>TRINITY_DN128189_c0_g1_i9 from sample A1 / Example spliced isoform

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGT
CTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAG
AGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGGCAATCAATATTTAAGGGTTG
GAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAACAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGTAGCTGTA
TGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTGCACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTGAAGGTGA
TAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGCCCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCATCTGCCT
TATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAATGAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAGCTGATGACATGGACAGTCTA
ATCAAACCAATTCGTTTGTTCCGGAAATAACAGTTTGGATCATCTCTTGAGCTGGTTATCAATGAATCATGTGCCAGAATGTCTTCTTTAGTTTGAGTGGTTATGCTTGATGTTACATTCTGTCAGCTTTGATGGGGTGTTCGATCTAATCTATCGAATTACCTTGATGGAGACAATAAGGTTTCCTTACTAGGTTATCA
TTTATTTTATTATTCAAATTCTATGATGATTATTGAGAGCTCATATAACTGTCCATTCAACAGGGTTTCTGCTTTTCTCATGGTTGGTCAAATTGACCAAGAACCATTTCCATGGTAATTCCTAACATGTTTTAGATCAATTTCCTGCTGCAATTTCTGCCACCTGTATAATAGCATCATTTTGAGTTGGTGATCTTCCT
GTACACTATTCAACCTGTCTCCTCATCTGTTTTCTATGTCAAAGACGTGGGTTTTGGGATGAGGAGGATGTTGCACTCAAGTGCCATGCAAGTTCACTCGGGGAACAGCAATGAACTGATCCAATGTAGAGATTCTTGGATCATTTTTTCAGGGCTAACTCAAGAATGGATTTAGGCTGCTGATGTGATGATTAATATGG
ATGTCTATAGAAGTGAGAAACAGTTTGTAGTACATTTTGCCCTTCCAAGAATTTGGAAGAGTTGAATGTTTTGCAAGTTTTTCTATTCTGTAAGTTCTTTTATTTAATTTAATTTTATGTTGAGTGGAATTTCTAAATGTGAAGAATGGTTCATATAAAATGGAGAGTCAAATGTTAACTGCTATCTCGATATTAGAAAT
GCTTAAATGTGCAATTTTCACATGGATTTGCACAATTTTATTGGAGTGAAGCTTTCACATATCACAAGACACACAACTGTAGTTGATGGTTTCTGTTCTATTGTGGTTGTCAAGAAATTTTGTTGGCAATTGTTCTACCTCTGCAGCATGCCCCCAGTGAAATCCATTTCAAGTAGCCCCTCCACTGTTAATGTTTACTA
TTCTGGATAGTGCCAGCAGAAGATCTTGAATTTGAGACTTTCTACACAAAAAATATTATCCTGAAAGAAGGTATTCGTG

>TRINITY_DN128189_c0_g1_i2 from sample A1 / Example unspliced isoform:

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGAAGCTCGATAGATTCTGAAGCTCAATCGAACCCAGT
ATAGAGATTTAGCAATACCTTTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAAT
CCCCATGTACACCCTCCTCCTCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGT
TACTGATGGAGACCATGAAATGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTT
CAAATGTCAGATTTCGATTGAATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCG
CCTCTTCAGTCAAGGGTTAAGTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATAT
GGCGGCGCTACCTAGTTTTTCTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGAC
TGGGAATCCCGGGCAACATGTCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTG
CGACAGCCATCTATGCAAGGTGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGA
TCATTCACTACCCAGAGAAGAGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTT
CTTCATACTTAGATGAGCTAGATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTT
GAAGAAAGACCACAGAAGAAGTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATC
ACGGGCATTCCAGGCTCAAAGCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGT
CATGCTTGAATCATTATTCTAATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATT
AGAAACCTATCAACAAGAGGAAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCA
GTCTCTTGCTCATGCACCGTTATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCG
AAAATGTTATGGCAGCTCAAGTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAA
ACCACTAAGGCCTCGGAGGTTCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACC
ACTTTTGCAATCAAAAAGGGTGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTAC
GTAGGTCAGGTGTTTTGAGTCTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAA
CCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGG
CAATCAATATTTAAGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAACAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACG
ATCGAGAAAAGGTAGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTGCACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAA
GGCTACTGTGCTGAAGGTGATAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGCCCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTAC
TAGTGAATCTGCATCTGCCTTATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAATGAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAG
CTGATGACATGGACAGTCTAATCAAACCAATTCGTTTGTTCCGGAAATAACAGGCAGTGACCGAACCATTAGGCCAAGTGAATCCTAAATCAAATGGACTATATCTACAATATCTACATGATTAATATGTATGCAGCAGATAGTGGTAATTGTCTGAAGTCTTTTTATTTCTACTTTTGTAGTTTGGATCATCTCTTGAG
CTGGTTATCAATGAATCATGTGCCAGAATGTCTTCTTTAGTTTGAGgtaagcttcatcctcttttcgcaaataatttcttgccaatcagcattctaagaattaattaaaatgttaaaatctaatatgattttgtttttggcagTGGTTATGCTTGATGTTACATTCTGTCAGCTTTGATGGGGTGTTCGATCTAATCTAT
CGAATTACCTTGATGGAGACAATAAGGTTTCCTTACTAGGTTATCATTTATTTTATTATTCAAATTCTATGATGATTATTGAGAGCTCATATAACTGTCCATTCAACAGGGTTTCTGCTTTTCTCATGGTTGGTCAAATTGACCAAGAACCATTTCCATGGTAATTCCTAACATGTTTTAGATCAATTTCCTGCTGCAAT
TTCTGCCACCTGTATAATAGCATCATTTTGAGTTGGTGATCTTCCTGTACACTATTCAACCTGTCTCCTCATCTGTTTTCTATGTCAAAGACGTGGGTTTTGGGATGAGGAGGATGTTGCACTCAAGTGCCATGCAAGTTCACTCGGGGAACAGCAATGAACTGATCCAATGTAGAGATTCTTGGATCATTTTTTCAGGG
CTAACTCAAGAATGGATTTAGGCTGCTGATGTGATGATTAATATGGATGTCTATAGAAGTGAGAAACAGTTTGTAGTACATTTTGCCCTTCCAAGAATTTGGAAGAGTTGAATGTTTTGCAAGTTTTTCTATTCTGTAAGTTCTTTTATTTAATTTAATTTTATGTTGAGTGGAATTTCTAAATGTGAAGAATGGTTCAT
ATAAAATGGAGAGTCAAATGTTAACTGCTATCTCGATATTAGAAATGCTTAAATGTGCAATTTTCACATGGATTTGCACAATTTTATTGGAGTGAAGCTTTCACATATCACAAGACACACAACTGTAGTTGATGGTTTCTGTTCTATTGTGGTTGTCAAGAAATTTTGTTGGCAATTGTTCTACCTCTGCAGCATGCCCC
CAGTGAAATCCATTTCAAGTAGCCCCTCCACTGTTAATGTTTACTATTCTGGATAGTGCCAGCAGAAGATCTTGAATTTGAGACTTTCTACACAAAAAATATTATCCTGAAAGAAGGTATTCGTG

Intron 2 GTGAGTTATC ... TGTTTGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.03	20	11	7
A2	13.89	31	5	5
A3	22.5	31	9	9
A4	19.27	44	12	9
A5	16.67	5	1	1
A6	4.76	10	1	0
A7	13.4	42	4	9
A8	18.18	18	3	5
A9	8.7	21	1	3
A10	23.81	16	7	3

>TRINITY_DN128189_c0_g1_i8 from sample A1 / Example spliced isoform

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGGTTTCAAACAGTCCGTTATCTCTATGGTTTTTGTGTACTAACTACTAAGTCGTTCTCTTTGTCAAATTCTTTTCAGAACTCTCACAAA
GTATAGTTTACTTATTTTTCAACGATAATTAACTTGAAGTGATTGGTGTGGGGATGCAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGTCTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGA
GGTGAATGAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGG
ATCCATCTAAGAGGACACTTCAGAGGATTCCTGGTGAGTTTTATTGTGTGGAAGTTTATTTGTTATCCAGTGCATGGCATATATCCTCATTTTTTTCTTATTCTTTTGAATGTGAATTTGAGTTTATTCTAGTCTATATAAATAGCAAATACCTATATTTGTGCACATACATGTCTGGGGGCATATTTTTGCTTGTTTCT
GAGCGCATGTCTGTGTGGGTTGCATCACATTAGCACTTCGAGATGCAAAAGGCTTGACTTGTCTGGTTGAAATACATGCAGCTAGTTGGTTTTTCTTCCATCATATAATTTGTTGATTTTATAGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAG
GAGGGTATCGATTGGTGGCAATCAGTAAGTGGACCTTCTCTTGTTCCCATTTG

>TRINITY_DN128189_c0_g1_i1 from sample A1 / Example unspliced isoform:

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGT
CTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGgtgagttatcctatccttgttaatttataatcaagatttactgggaatttccttgatccatatagatgatatttggctctatatgtttgaaagGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGA
AGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCA
TCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGGCAATCAATATTTAAGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAA
CAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGTAGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTG
CACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTGAAGGTGATAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGC
CCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCATCTGCCTTATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAAT
GAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAGCTGATGACATGGACAGTCTAATCAAACCAATTCGTTTGTTCCGGAAATAACAGTTTGGATCATCTCTTGAGCTGGTTATCAATGAATCATGTGCCAGAATGTCTTCTTTAGTTTGAGTGGTA

Intron 3 GTAAGTGGAC ... ATTTGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	36.17	30	12	17
A2	45.16	17	4	14
A3	25	15	4	5
A4	29.17	17	0	7
A5	52.38	10	0	11
A6	15	17	0	3
A7	21.74	36	0	10
A8	25.49	38	2	13
A9	28.95	27	1	11
A10	27.66	34	2	13

>TRINITY_DN46230_c0_g1_i9 from sample A8 / Example spliced isoform

AGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGAGTCTTCTTCATACTTAGAGGAGCTAGATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCAACGAAGAATGATAGTC
CTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAAGTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACA
CCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAAGCTCACAGCCAGATAACAAGTTCTCACAAAATCTGATGTCAGCACCTTCGTTGCCCCAAAGCAGGCCTAC
TTTTGCTCCCCCTATCCGAACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCATCGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCTAATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGA
ATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGGAAACATAAACAACATTGCAGCTGGTTCGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTA
TATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGTTATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAG
TAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAAGTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAG
CATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGTTCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATG
GCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGGTGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGC
TGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGTCTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGAAGCAA
CAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACA
CTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGGCAATCAATATTTAAGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATG
GAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAACAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGTAGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGC
GCATGGAAGATTGCTCATATTTTCTACAGGGTTATTGCACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTGAAGGTGATAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCA
ACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGCCCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCATCTGCCTTATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCAT
AAGTCTTGATGAATCAGGGGAAGATGCTAATAATAATGAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAGCTGATGACATGGACAGTCTAATCAAACCAATTCGTTTGTTCCGGAAATAACAGTTTGGATCATCTCTTGAGCTGGTTATCAATGAATCAT
GTGCCAGGATGTCTTCTTTAGTTTGAGGTAAGCTTCATCCTCTTTTCGCAAATAATTTCTTGCCAATCAGCATTCTAAGAATTAATTAAAATGTTAAAATCTAATATGATTTTGTTTTTGGCAGTGGTTATGCTTGATGTTACATTCTGTCAGCTTTGATGGGGTGTTCGATCTAATCTATCGAATTACCTTGATGGAGA
CAATAAGGTTTCCTTACTAGGTTATCATTTATTTTATTATTCAAATTCTATGATGATTATTGAGAGCTCATATAACTGTCCATTCAACAGGGTTTCTGCTTTTCTCATGGTTGGTCAAATTGACCAAGAACCATTTCCATGGTAATTCCTAACATGTTTTAGATCAATTTCCTGCTGCAATTTCTGCCACCTGTATAATA
GCATCATTTTGAGTTGGTGATCTTCCTGTACACTATTCAACCTGTCTCCTCATCTGTTTTCTATGTCAAAGACGTGGGTTTTGGGATGAGGAGGATGTTGCACTCAAGTGCCATGCAAGTTCACTCGGGGAACAGCAATGAACTGATCCAATGTAGAGATTCTTGGATCATTTTTTCAGGGCTAACTCAAGAATGGATTT
AGGCTGCTGATGTGATGATTAATATGGATGTCTATAGAAGTGAGAAACAGTTTGTAGTACATTTTGCCCTTCCAAGAATTTGGAAGAGTTGAATGTTTTGCAAGTTTTTCTATTCTGTAAGTTCTTTTATTTAATTTAATTTTATGTTGAGTGGAATTTCTAAATGTGAAGAATGGTTCATATAAAATGGAGAGTCAAAT
GTTAACTGCTATCTCGATATTAGAAATGCTTAAATGTGCAATTTTCACATGGATTTGCACAATTTTATTGGAGTGAAGCTTTCACATATCACAAGACACACAACTGTAGTTGATGGTTTCTGTTCTATTGTGGTTGTCAAGAAATTTTGTTGGCAATTGTTCTACCTCTGCAGCATGCCCCCAGTGAAATCCATTTCAAG
TAGCCCCTCCACTGTTAATGTTTACTATTCTGGATAGTGCCAGCAGAAGATCTTGAATTTGAGACTTTCTACACAAAAAATATTATCCTGAAAGAAGGTATTCGTGCTTGGATGGCGGTTCAAGATCAGCCTCATGAAAACCTTATATTCCCTGAGGAGCTTGGATTTCCATGGTTTAGATGGAATCTTGAACCTATCTG
GTCTGGTGATGATATAGAAGTATCCCATATCAAATTTATCCAGAAGCCCACTCATGACCCCTCTAAGAATGAATATTGAAAAAACCTAATCCATCTAGAGGGTCGAATGATTGGATAAATAAGATATAGAAATTAAAGGACGGGC

>TRINITY_DN46230_c0_g1_i4 from sample A8 / Example unspliced isoform:

GAGGACACTTCAGAGGATTCCTGGTGAGTTTTATTGTGTGGAAGTTTATTTGTTATCCAGTGCATGGCATATATCCTCATTTTTTTCTTATTCTTTTGAATGTGAATTTGAGTTTATTCTAGTCTATATAAATAGCAAATACCTATATTTGTGCACATACATGTCTGGGGGCATATTTTTGTTTGTTTCTGAGCGCATGT
CTGTGTGGGTTGCATCACATTAGCACTTCGAGATGCAAAAGGCTTGACTTGTCTGGTTGAAATACATGCAGCTAGTTGGTTTTTCTTCCATCATATAATTTGTTGATTTTATAGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCG
ATTGGTGGCAATCAgtaagtggaccttctcttgttcccatttggagttgtataatacagagtcatctgaaatggatctatgtttgtttttcaagcatttgagggttagtttatataatgttagggtttaaatcatggagctactactgtattttggacttcaataacaagttctttgcatgggatttgaacagATATTTA
AGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAACAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGT
AGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTGCACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTG
AAGGTGATAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGCCCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCA
TCTGCCTTATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAATGAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCGGCTGATGACATGGA
CAGTCTAATCAAACCAATTCGTTTGTTCCGGAAATAACAGGCAGTGACCGAACCATTAGGCCAAGTGAATCCTAAATCAAATGGACTATATCTACAATATCTACATGATTAATATGTATGCAGCAGATAGTGGTAATTGTCTGAAGTCTTTTTATTTCTACTTTTGTAGTTTGGATCATCTCTTGAGCTGGTTATCAATG
AATCATGTGCCAGGATGTCTTCTTTAGTTTGAGTGGTTATGCTTGATGTTACATTCTGTCAGCTTTGATGGGGTGTTCGATCTAATCTATCGAATTACCTTGATGGAGACAATAAGG

Intron 4 GTGAGTTTTA ... GATTTTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.51	43.7	22.6	14.1
A2	38.77	16	11.5	8.9
A3	25.94	28.7	12	8.3
A4	20.92	23.7	12.2	0.7
A5	37.83	5	3.3	3
A6	13.34	11	3.4	0
A7	16.99	30.7	8.1	4.4
A8	9.99	40.3	7.3	1.6
A9	16.3	24.7	7.3	2.3
A10	18.18	34	10.4	4.4

>TRINITY_DN128189_c0_g1_i1 from sample A1 / Example spliced isoform

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGT
CTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGTGAGTTATCCTATCCTTGTTAATTTATAATCAAGATTTACTGGGAATTTCCTTGATCCATATAGATGATATTTGGCTCTATATGTTTGAAAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGA
AGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCA
TCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGGCAATCAATATTTAAGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAA
CAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGTAGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTG
CACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTGAAGGTGATAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGC
CCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCATCTGCCTTATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAAT
GAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAGCTGATGACATGGACAGTCTAATCAAACCAATTCGTTTGTTCCGGAAATAACAGTTTGGATCATCTCTTGAGCTGGTTATCAATGAATCATGTGCCAGAATGTCTTCTTTAGTTTGAGTGGTA

>TRINITY_DN128189_c0_g1_i8 from sample A1 / Example unspliced isoform:

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGGTTTCAAACAGTCCGTTATCTCTATGGTTTTTGTGTACTAACTACTAAGTCGTTCTCTTTGTCAAATTCTTTTCAGAACTCTCACAAA
GTATAGTTTACTTATTTTTCAACGATAATTAACTTGAAGTGATTGGTGTGGGGATGCAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGTCTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGA
GGTGAATGAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGG
ATCCATCTAAGAGGACACTTCAGAGGATTCCTGgtgagttttattgtgtggaagtttatttgttatccagtgcatggcatatatcctcatttttttcttattcttttgaatgtgaatttgagtttattctagtctatataaatagcaaatacctatatttgtgcacatacatgtctgggggcatatttttgcttgtttct
gagcgcatgtctgtgtgggttgcatcacattagcacttcgagatgcaaaaggcttgacttgtctggttgaaatacatgcagctagttggtttttcttccatcatataatttgttgattttatagATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAG
GAGGGTATCGATTGGTGGCAATCAGTAAGTGGACCTTCTCTTGTTCCCATTTG

Intron 5 GTATTGTAAA ... TTACTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.08	40	12	2
A2	23.91	35	11	0
A3	5.66	50	3	0
A4	20.83	19	5	0
A5	22.22	14	4	0
A6	9.68	56	6	0
A7	11.88	89	12	0
A8	11.58	84	11	0
A9	12.5	63	9	0
A10	11.63	76	10	0

>TRINITY_DN119676_c1_g1_i1 from sample A2 / Example spliced isoform

TGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTATAGTTAAGATTGGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAAT
CATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCTTTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAA
TCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCCTCCTCCTCCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAA
GCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAATGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCA
TCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTGAATGAAAGGTTTGAGTTCGAGAAAATCAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTTTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGA
GGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAAGTGGCAGCAGAGTCAGAGCCATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTG
CCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTTCTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGC
AGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATGTCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCA
GCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGGTGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTAT
GATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAGAGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGATTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTC
AGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGAGTCTTCTTCATACTTAGAGGAGCTAGATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCAACGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTC
CACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAAGTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCT
GATGTTCAACTGCTTCAAAATAGAACTCTGGGGTCACATGTAACTCAAGAATCATCACGGGCATTCCAGACTCAAAGCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGAACCAAAGGAATAGCTAATCCAGTACATGCCACAAG
GCCTCGAACCTGGCATCGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCTAATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAG
ATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGGAAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGG
ACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGTTATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAA
TGTTCCATCTAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAAGTGATTTATCTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCACCATCCAATGGCCTGGTACAAGATCATT
ACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGTTCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTT
TTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGGTGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAA
GCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGTCTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCG
ATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTG
AGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGGCAATCAATATTTAAGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAACAACAGTATTGCCAATT
TTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGTAGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTGCACCAAAGAGAATTGTC
CATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTGAAGGTGATAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGCCCAAAGTTTCAAGTGAG
CATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCATCTGCCTTATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAATGAGTTGATCCAAGGTGC
TCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAGCTGATGACATGGACAGTCTAACCAAACCAATTCGTTTGTTCCGGAAATAACAGTTTGGATCATCTCTTGAGCTGGTTATCAATGAATCATGTGCCAGAATGTCTTCTTTAGTTTGAGTGGTTATGCTTGATGTTACATTCTGTC
AGCTTTGATGGGGTGTTCGATCTAATCTATTGAATTACCTTGATGGAGACAATAAGGTTTCCTTACTAGGTTATCATTTATTTTATTATTCAAATTCTATGATGATTATTGAGAGCTCATATAACTGTCCATTCAACAGGGTTTCTGCTTTTCTCATGGTTGGTCAAATTGACCAAGAACCATTTCCATGGTAATTCCTA
ACATGTTTTAGATCAATTTCCTGCTGCAATTTCTGCCACCTGTATAATAGCATCATTTTGAGTTGGTGATCTTCCTGTACACTATTCAACCTGTCTCCTCATCTGTTTTCTATGTCAAAGACGTGGGTTTTGGGATGAGGAGGATGTTGCACTCAAGTGCCATGCAAGTTCACTCGGGGAACAGCAATGAACTGATCCAA
TGTAGAGATTCTTGGATCATTTTTTCAGGGCTAACTCAAGAATGGATTTAGGCTGCTGATGTGATGATTAATATGGATGTCTATAGAAGTGAGAAACAGTTTGTAGTACATTTTGCCCTTCCAAGAATTTGGAAGAGTTGAATGTTTTGCAAGTTTTTCTATTCTGTAAGTTCTTTTATTTAATTTAATTTTATGTTGAG
TGGAATTTCTAAATGTGAAGAATGGTTCATATAAAATGGAGAGTCAAATGTTAACTGCTATCTCGATATTAGAAATGCTTAAATGTGCAATTTTCACATGGATTTGCACAATTTTATTGGAGTGAAGCTTTCACATATCACAAGACACACAACTGTAGTTGATGGTTTCTGTTCTATTGTGGTTGTCAAGAAATTTTGTT
GGCAATTGTTCTACCTCTGCAGCATGCCCCCAGT

>TRINITY_DN119676_c1_g1_i5 from sample A2 / Example unspliced isoform:

ATATTTAAGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAACAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAG
AAAAGGTAGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGgtattgtaaaactttaactccatacactaggcttggagatgtctagataaaagcaaaatgttgcagttgtcaagatcttatagtaggagtaattatgctgtatcttacttgttctgttatttttactca
cagATTATTCCAG

Intron 6 GTTTCCTTAC ... CATTCAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	78.89	8.8	39.8	23
A2	74.47	13.6	36.7	37
A3	73.79	16.3	50.4	36
A4	69.97	14.3	20.7	37
A5	55.8	8.2	8.3	5
A6	72.85	52.3	125.1	131.3
A7	73.62	45.8	145.8	93.9
A8	69.76	66.5	165.8	127.6
A9	76.39	39.4	117.2	121.7
A10	79.53	24.1	112.9	66

>TRINITY_DN128189_c0_g1_i14 from sample A1 / Example spliced isoform

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGT
CTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAG
AGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGGCAATCAATATTTAAGGGTTG
GAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAACAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGTAGCTGTA
TGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTGCACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTGAAGGTGA
TAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGCCCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCATCTGCCT
TATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAATGAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAGCTGATGACATGGACAGTCTA
ATCAAACCAATTCGTTTGTTCCGGAAATAACAGGCAGTGACCGAACCATTAGGCCAAGTGAATCCTAAATCAAATGGACTATATCTACAATATCTACATGATTAATATGTATGCAGCAGATAGTGGTAATTGTCTGAAGTCTTTTTATTTCTACTTTTGTAGTTTGGATCATCTCTTGAGCTGGTTATCAATGAATCATG
TGCCAGAATGTCTTCTTTAGTTTGAGTGGTTATGCTTGATGTTACATTCTGTCAGCTTTGATGGGGTGTTCGATCTAATCTATCGAATTACCTTGATGGAGACAATAAGGGTTTCTGCTTTTCTCATGGTTGGTCAAATTGACCAAGAACCATTTCCATGGTAATTCCTAACATGTTTTAGATCAATTTCCTGCTGCAAT
TTCTGCCACCTGTATAATAGCATCATTTTGAGTTGGTGATCTTCCTGTACACTATTCAACCTGTCTCCTCATCTGTTTTCTATGTCAAAGACGTGGGTTTTGGGATGAGGAGGATGTTGCACTCAAGTGCCATGCAAGTTCACTCGGGGAACAGCAATGAACTGATCCAATGTAGAGATTCTTGGATCATTTTTTCAGGG
CTAACTCAAGAATGGATTTAGGCTGCTGATGTGATGATTAATATGGATGTCTATAGAAGTGAGAAACAGTTTGTAGTACATTTTGCCCTTCCAAGAATTTGGAAGAGTTGAATGTTTTGCAAGTTTTTCTATTCTGTAAGTTCTTTTATTTAATTTAATTTTATGTTGAGTGGAATTTCTAAATGTGAAGAATGGTTCAT
ATAAAATGGAGAGTCAAATGTTAACTGCTATCTCGATATTAGAAATGCTTAAATGTGCAATTTTCACATGGATTTGCACAATTTTATTGGAGTGAAGCTTTCACATATCACAAGACACACAACTGTAGTTGATGGTTTCTGTTCTATTGTGGTTGTCAAGAAATTTTGTTGGCAATTGTTCTACCTCTGCAGCATGCCCC
CAGTGAAATCCATTTCAAGTAGCCCCTCCACTGTTAATGTTTACTATTCTGGATAGTGCCAGCAGAAGATCTTGAATTTGAGACTTTCTACACAAAAAATATTATCCTGAAAGAAGGTATTCGTG

>TRINITY_DN128189_c0_g1_i7 from sample A1 / Example unspliced isoform:

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGT
CTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAG
AGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGGCAATCAATATTTAAGGGTTG
GAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAACAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGTAGCTGTA
TGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTGCACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTGAAGGTGA
TAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGCCCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCATCTGCCT
TATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAATGAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAGCTGATGACATGGACAGTCTA
ATCAAACCAATTCGTTTGTTCCGGAAATAACAGGCAGTGACCGAACCATTAGGCCAAGTGAATCCTAAATCAAATGGACTATATCTACAATATCTACATGATTAATATGTATGCAGCAGATAGTGGTAATTGTCTGAAGTCTTTTTATTTCTACTTTTGTAGTTTGGATCATCTCTTGAGCTGGTTATCAATGAATCATG
TGCCAGAATGTCTTCTTTAGTTTGAGTGGTTATGCTTGATGTTACATTCTGTCAGCTTTGATGGGGTGTTCGATCTAATCTATCGAATTACCTTGATGGAGACAATAAGgtttccttactaggttatcatttattttattattcaaattctatgatgattattgagagctcatataactgtccattcaacagGGTTTCTG
CTTTTCTCATGGTTGGTCAAATTGACCAAGAACCATTTCCATGGTAATTCCTAACATGTTTTAGATCAATTTCCTGCTGCAATTTCTGCCACCTGTATAATAGCATCATTTTGAGTTGGTGATCTTCCTGTACACTATTCAACCTGTCTCCTCATCTGTTTTCTATGTCAAAGACGTGGGTTTTGGGATGAGGAGGATGT
TGCACTCAAGTGCCATGCAAGTTCACTCGGGGAACAGCAATGAACTGATCCAATGTAGAGATTCTTGGATCATTTTTTCAGGGCTAACTCAAGAATGGATTTAGGCTGCTGATGTGATGATTAATATGGATGTCTATAGAAGTGAGAAACAGTTTGTAGTACATTTTGCCCTTCCAAGAATTTGGAAGAGTTGAATGTTT
TGCAAGTTTTTCTATTCTGTAAGTTCTTTTATTTAATTTAATTTTATGTTGAGTGGAATTTCTAAATGTGAAGAATGGTTCATATAAAATGGAGAGTCAAATGTTAACTGCTATCTCGATATTAGAAATGCTTAAATGTGCAATTTTCACATGGATTTGCACAATTTTATTGGAGTGAAGCTTTCACATATCACAAGACA
CACAACTGTAGTTGATGGTTTCTGTTCTATTGTGGTTGTCAAGAAATTTTGTTGGCAATTGTTCTACCTCTGCAGCATGCCCCCAGTGAAATCCATTTCAAGTAGCCCCTCCACTGTTAATGTTTACTATTCTGGATAGTGCCAGCAGAAGATCTTGAATTTGAGACTTTCTACACAAAAAATATTATCCTGAAAGAAGG
TATTCGTG

Intron 7 GTTTCAAACA ... GGGGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30	21	8	10
A2	28.21	14	4	7
A3	30.34	31	14	13
A4	29.73	13	5	6
A5	20	12	3	3
A6	52.94	4	3	6
A7	14.61	38	5	8
A8	15.79	32	4	8
A9	21.95	16	4	5
A10	20.93	17	2	7

>TRINITY_DN128189_c0_g1_i1 from sample A1 / Example spliced isoform

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGT
CTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGTGAGTTATCCTATCCTTGTTAATTTATAATCAAGATTTACTGGGAATTTCCTTGATCCATATAGATGATATTTGGCTCTATATGTTTGAAAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGA
AGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCA
TCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGGCAATCAATATTTAAGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAA
CAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGTAGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTG
CACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTGAAGGTGATAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGC
CCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCATCTGCCTTATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAAT
GAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAGCTGATGACATGGACAGTCTAATCAAACCAATTCGTTTGTTCCGGAAATAACAGTTTGGATCATCTCTTGAGCTGGTTATCAATGAATCATGTGCCAGAATGTCTTCTTTAGTTTGAGTGGTA

>TRINITY_DN128189_c0_g1_i8 from sample A1 / Example unspliced isoform:

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGgtttcaaacagtccgttatctctatggtttttgtgtactaactactaagtcgttctctttgtcaaattcttttcagaactctcacaaa
gtatagtttacttatttttcaacgataattaacttgaagtgattggtgtggggatgcagCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGTCTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGA
GGTGAATGAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGG
ATCCATCTAAGAGGACACTTCAGAGGATTCCTGGTGAGTTTTATTGTGTGGAAGTTTATTTGTTATCCAGTGCATGGCATATATCCTCATTTTTTTCTTATTCTTTTGAATGTGAATTTGAGTTTATTCTAGTCTATATAAATAGCAAATACCTATATTTGTGCACATACATGTCTGGGGGCATATTTTTGCTTGTTTCT
GAGCGCATGTCTGTGTGGGTTGCATCACATTAGCACTTCGAGATGCAAAAGGCTTGACTTGTCTGGTTGAAATACATGCAGCTAGTTGGTTTTTCTTCCATCATATAATTTGTTGATTTTATAGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAG
GAGGGTATCGATTGGTGGCAATCAGTAAGTGGACCTTCTCTTGTTCCCATTTG

Intron 8 GCAGTGACCG ... ACTTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.84	35.4	39	28.5
A2	35.4	21.8	16	7.9
A3	40.54	22.5	15	15.6
A4	37.39	19.9	9	14.6
A5	58.71	6.3	10	7.9
A6	32.87	86.8	51.1	33.6
A7	43.17	74.7	70	43.3
A8	39.5	83.1	66.6	41.6
A9	37.91	70.3	59.9	25.9
A10	42.97	46.9	51.9	18.8

>TRINITY_DN128189_c0_g1_i1 from sample A1 / Example spliced isoform

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGCTCAATCGAACCCAGTATAGAGATTTAGCAATACCT
TTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAATCCCCATGTACACCCTCCTCC
TCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGTTACTGATGGAGACCATGAAA
TGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTTCAAATGTCAGATTTCGATTG
AATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCGCCTCTTCAGTCAAGGGTTAA
GTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATATGGCGGCGCTACCTAGTTTTT
CTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGACTGGGAATCCCGGGCAACATG
TCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTGCGACAGCCATCTATGCAAGG
TGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGATCATTCACTACCCAGAGAAG
AGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTTCTTCATACTTAGATGAGCTA
GATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTTGAAGAAAGACCACAGAAGAA
GTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATCACGGGCATTCCAGGCTCAAA
GCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGTCATGCTTGAATCATTATTCT
AATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATTAGAAACCTATCAACAAGAGG
AAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCAGTCTCTTGCTCATGCACCGT
TATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCGAAAATGTTATGGCAGCTCAA
GTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAAACCACTAAGGCCTCGGAGGT
TCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACCACTTTTGCAATCAAAAAGGG
TGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTACGTAGGTCAGGTGTTTTGAGT
CTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGTGAGTTATCCTATCCTTGTTAATTTATAATCAAGATTTACTGGGAATTTCCTTGATCCATATAGATGATATTTGGCTCTATATGTTTGAAAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGA
AGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAACCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCA
TCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGGCAATCAATATTTAAGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAA
CAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACGATCGAGAAAAGGTAGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTG
CACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAAGGCTACTGTGCTGAAGGTGATAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGC
CCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTACTAGTGAATCTGCATCTGCCTTATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAAT
GAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAGCTGATGACATGGACAGTCTAATCAAACCAATTCGTTTGTTCCGGAAATAACAGTTTGGATCATCTCTTGAGCTGGTTATCAATGAATCATGTGCCAGAATGTCTTCTTTAGTTTGAGTGGTA

>TRINITY_DN128189_c0_g1_i2 from sample A1 / Example unspliced isoform:

GGCATATCGTTAATATCTCCATCATAATTGCCGCAACACAGAGGCAAACTTGTAAGTGAAAGACAAAGTGAATTTCGTAGAAGCACGGCTTGAGTGACGTCCTCGTTAGAAAGACCGACAGTAATTGTTAATGAGGTCAATTATCATTTGATCACTAAATTGTAGTTGTGATGCTTTCACACTAAATTGTAGTTCAGATT
GGATGCACATTAGTAAAGCCAGCACTCAATCGTGCTTGATATTTAACTAATGGAACATTTGGAGGTTGAAAGCATTTGGATTCACGAAGAGGACCGACGACGAAACAAGGAGACTGCGAGTCAATCATTGGTAGATGTTCGATCGTTTATATCCGTTTTTGAAGAAGCTCGATAGATTCTGAAGCTCAATCGAACCCAGT
ATAGAGATTTAGCAATACCTTTCGACCATCCATTATTTTGTTATTTCCAAAATTCACAAACCCCAAGGGTTGGAGTGCAGTGGACTGCAGTAATGGACGCGAGGGCTCGCCAATTGCAATTCCAATTCCAGTTGCGCAATCCAAATCCACTGGTAGACTTTCCAGGAGGCGACAGAAGGTTTCGTCCTCCTCATCCCAAT
CCCCATGTACACCCTCCTCCTCCTCCTCCCCCCCCCCCGTTTGTTCTCCATGGAAACAATAATAGTATGAAGGATCACCATTTGGGTATTCAGAGTCCGAGGTACCCTTCTGCACCAGTTCCTCTAGAACCATGGGACCGCGAAGCTATCATGGCGTACAGAAACAGAGTCAATGCCAATTCTGCCAATCGGGCAGCCGT
TACTGATGGAGACCATGAAATGCCCATCAGGAGTAATTTACATAGAGCCATGGAGCGCCCAGAGGACATTTCTCCCACGGTGCGCCAACAGGATTATCGGGATTGGCCTGCCCAGCGATTCAGAGAGAACCACCACCATCGTCATCATCAGGTAAGAGGAAATCCAAACCCCAACCCTATTCTGGAACAGAATTCTTCTT
CAAATGTCAGATTTCGATTGAATGAAAGGTTTGAGTTCGAGAAAATTAGTAGGAAAAGAAGTCTAGAAGAAGATGGCGATGGGGAGGAGTCTTTGCGGAGAAGACCAAAGTTTGTGGATTTGGGTTTTGAGAGGACCGAGAGGAGGGATGCTCAATTTGATTACTCCCCTCCACGGGATATGTTTTTGCCTCCTCCCTCG
CCTCTTCAGTCAAGGGTTAAGTGGCAGCAGAGTCAGAGCGATATGCAGTTCAATAATAATTTCATTGAGCCAGGGGACCAGAGGAAGATGGTCCCAGAGACAGAATCTATTCGTTCTGCTGGCAATTCTTATGGTTTGGCCTTGCCTGGATCAAGAGAATTTCCAATAAGGGCAGAAAGTTTTTGTGATGAGGATCATAT
GGCGGCGCTACCTAGTTTTTCTAAAGGGCAAACCATGCAGACCCCACTGCAATTTGCAGCAGAGGATGTGGAAGTTGTCTCCGAAGAAGATGATCTTTTTCCTCAAGTTGTAAAAGAATATCCAACTTCTGCAGCTGCTGGTGCAGGGTTACATCAATTGGAAAAGCCAGCAACAAAGGATCACAACAAAGGCAAAAGAC
TGGGAATCCCGGGCAACATGTCTTCAAAGAATCAGAAAACCATAATGAAAAATAAAACTGCATCGCGGGATCAACCGAACAGGAAGAAACATAAGCGAAAAAGGAATCCAGTCGATGCATTTTCTGCTCAGACGCCTAGTAGCAGCCTAGCATCGGTCAAAGATGGGAGAATATCACAAAATGGATCTGTCGCCAATTTG
CGACAGCCATCTATGCAAGGTGAACAATCTGCTTACAAATTTGAAAAGATAGGTTCTGCCCAGCGTGAAAGGAACACAGCAGATCTTTCTGTTGTTTTTGGACCCAGTGGTATGATAGCACAGGAAGTTGTTACTTCAGATTATGATACTCTTCCTGAATACAGGACCAATTCATCGAGTTCTAAAGGATTTGTCCAAGA
TCATTCACTACCCAGAGAAGAGATTGAAGAACAAGCCAAGAAAGAAGTCATAGACATTCAAGGTGTTGTACTTCAGAAAAATGGGTTGGCAGACAATCCGAATACAGGTTCTCAGGCAAAAACCAGGCTCATGAATATTATTTCAGAAACCATTTCATGTGAGAAAGATTCAATTTTAGAAGCTGATTCATTTGGGTCTT
CTTCATACTTAGATGAGCTAGATGATAAAAATATAACTTTAGCAATGCAGTCCAAGGACAGGGCTGTAGCACCATCGAAGAATGATAGTCCTGCCTCTACAAAGGTCAATCCAGTTAATGATGGACCAACCACCATGGAATTTCCACAAGGTACGTCTTTTGCATCCTCAGATGGCAAGGCCACTGACATGGCAAAATTT
GAAGAAAGACCACAGAAGAAGTTGAGAGTTGAGCAGACAGACAAGCTTGCCATAACAGACAGTGAAAAGATGGAGCAAGGTTTTTTGACACCTGCATTTGTACAAACTGGTACTGAAGTAACTGTGTCACAGGGGCCTGTTGCTGATGTTCAACTGCTTCAAAATAGAACTCTGGGATCACATGTAACTCAAGAATCATC
ACGGGCATTCCAGGCTCAAAGCTCACAGCCAGATAACAAGTTTTCACAAAATCTGATGTCAGCATCTTCGTTGCCCCAAAGCAGGCCTACTTTTGCTCCCCCTATCCGGACCAAAGGAATAGCTAATCCAGTACATGCCACAAGGCCTCGAACCTGGCAACGGACTGGTAATGTGACTGGTTCTGCTATGGTATCTAGGT
CATGCTTGAATCATTATTCTAATGCTTTGGGTGAGCAGGTAGTGGCCAAGGTGGAGAATTCCCAATCACCAGCTTATTTTCGCAAGGGGAATAGTCTTGTAAGGGCTTCCCGTTCTTCCATTGGGTTCTCTTCTACAGTATCAGATTCGTCTGCATCCTTGAATGCTAATAAGGCATGTCTACCAAAATCTTCTCACATT
AGAAACCTATCAACAAGAGGAAACATAAACAACATTGCAGCTGGTTTGGGCACTAATGCTGTGTGCACAAGGCCTTCTTCTGCTACAGTATATGGGAGTGTAATGGCAGTTGAATGTGCTACACCTGCTTTAGAGAGACCTAGGACCCCACCTCTAGCACAAACTGTGAAATTGCCGGTCTGTGTAACCAGCTCTCCTCA
GTCTCTTGCTCATGCACCGTTATCAAGTGTTCCCTCCAAATTGCATATAATGATTACTGATTCATCTGTTGTGGATGCTGTCAAGATTAGTAATGAAAAGGTGCCTAGTCTGGTCAACAAATCTGTTGAAAGCCAAGTAACGAATGTTCCATCCAACAATTTGTCACTAGGTAGAAGCAATCTTAATGAAGATCAGGTCG
AAAATGTTATGGCAGCTCAAGTGATTTATTTGAAGCGAAAGTCAAATCAGTTAATTGCAGCTCCAACTTGTAAATTGCACTGTTCAACAGCATCTATTAGTGAAGGCAGCCAATCGCCATCCAATGGCCTGGTACAAGATCATTACTATAAGCGAAAGAAGAACCAGCTTGTTCGCAGCAGCTTGCCATTGGACACCAAA
ACCACTAAGGCCTCGGAGGTTCCTGGTTCAAGCTGTTTTTCGGGTACAAAGGAGTTGAAGGTTCCAAGATTGAAAACTCAACAAAATATGGCAGTATTCCAAACAAAGCTTGGCAGAGTTTTGAAGCAGAAGAAACATTGTGTTTTGCAATCTTCACGGGTATGGACTTTGAGTCAAACACAACATCCTTCGAGTTTACC
ACTTTTGCAATCAAAAAGGGTGGTTCCTTTGCTCTTTCCATGGAAAAGGCCATCATATGGAAGATCAGTTCGTTTAGGAAAAATCAAAGCTGTTCCAGATTACAACAAAGGCTCTTCACTTTCTCTAATGAGCAAGAAGCTGAAGCGTCTTCTGAAGAGGGACACTGTTTACACAAAATCACTTGATGGTTTCTCTTTAC
GTAGGTCAGGTGTTTTGAGTCTTGGAGGATCTAACTTAAAGTGGACCAAGTCTCTTGAACAACGCTCAAAAGAGGTGAATGAGGAAGCAACAATGGCAGTAGCTGCAGCAGCGAAGAAGAAAAGGGAAGAGAAAGCTATTGCCGATGTTGTTACTGCCACCAAGGTCAAAACATTGAAGCAGGCCACTAAAGCAATGAAA
CCACGTGCTCGCAAGTCCAGAGAAATCACAGGAGAACGCATTGTCCGTGTTGGTTTGTTTCGATATAAGATGGATCCATCTAAGAGGACACTTCAGAGGATTCCTGATGAAGAGTCTACTTCTAGCATCGATGCTTCATTGGTGAGTCAAACAATTAATACTGCAAAGGTTTTCACTCCAAGGAGGGTATCGATTGGTGG
CAATCAATATTTAAGGGTTGGAAATGGAAACCAACTTGTTAGGGATCCAAAGACAATAAGCCGTGCCCTAGCTAGTGAGAAAGTTAGATGGAGTTTACACACTGCCAGATGCCGTTTGGCTAAAAAACAACAGTATTGCCAATTTTTTACAAGATTTGGCAAATGCAATAAGGAGGGAGGTAAATGTCCTTACATTCACG
ATCGAGAAAAGGTAGCTGTATGCACTAAGTTCCTGAGGGGTACCTGTTCCAATGTCAACTGTGAGTTGACTCACAAGATTATTCCAGAGCGCATGGAAGATTGCTCATATTTTCTACAGGGTTATTGCACCAAAGAGAATTGTCCATACAGGCATGTAAATGTCAACCCAAAAGCTTCAGTTTGTGATGGATTTCTCAAA
GGCTACTGTGCTGAAGGTGATAAGTGTAATAAGAAGCACACATATGTATGCCCAGAGTATGCTGCAACAGGAGAATGTAAAGAAAGGTCAACTTGTAAGCTTCATCACCCAAAGAAGAAAGATAAGCCCAAAGTTTCAAGTGAGCATAAAGGTGGTACTAATACTAAAAGGCGCTACTTTGTAGCAGGGGATTTCGCTAC
TAGTGAATCTGCATCTGCCTTATTTAGTTTGCATTGTGCACAAACAGCTCTTGAAATGCCATCAGGGTCACCTGAGTGCCTTGAGTTCATAAGTCTTGATGAATCAGGGGAAGATGCTAATAATAATGAGTTGATCCAAGGTGCTCTTGGTCCCATGGAAAGGGTTGCATCAGATTTTTTAAGTAATCTGAGATTACCAG
CTGATGACATGGACAGTCTAATCAAACCAATTCGTTTGTTCCGGAAATAACAGgcagtgaccgaaccattaggccaagtgaatcctaaatcaaatggactatatctacaatatctacatgattaatatgtatgcagcagatagtggtaattgtctgaagtctttttatttctacttttgtagTTTGGATCATCTCTTGAG
CTGGTTATCAATGAATCATGTGCCAGAATGTCTTCTTTAGTTTGAGGTAAGCTTCATCCTCTTTTCGCAAATAATTTCTTGCCAATCAGCATTCTAAGAATTAATTAAAATGTTAAAATCTAATATGATTTTGTTTTTGGCAGTGGTTATGCTTGATGTTACATTCTGTCAGCTTTGATGGGGTGTTCGATCTAATCTAT
CGAATTACCTTGATGGAGACAATAAGGTTTCCTTACTAGGTTATCATTTATTTTATTATTCAAATTCTATGATGATTATTGAGAGCTCATATAACTGTCCATTCAACAGGGTTTCTGCTTTTCTCATGGTTGGTCAAATTGACCAAGAACCATTTCCATGGTAATTCCTAACATGTTTTAGATCAATTTCCTGCTGCAAT
TTCTGCCACCTGTATAATAGCATCATTTTGAGTTGGTGATCTTCCTGTACACTATTCAACCTGTCTCCTCATCTGTTTTCTATGTCAAAGACGTGGGTTTTGGGATGAGGAGGATGTTGCACTCAAGTGCCATGCAAGTTCACTCGGGGAACAGCAATGAACTGATCCAATGTAGAGATTCTTGGATCATTTTTTCAGGG
CTAACTCAAGAATGGATTTAGGCTGCTGATGTGATGATTAATATGGATGTCTATAGAAGTGAGAAACAGTTTGTAGTACATTTTGCCCTTCCAAGAATTTGGAAGAGTTGAATGTTTTGCAAGTTTTTCTATTCTGTAAGTTCTTTTATTTAATTTAATTTTATGTTGAGTGGAATTTCTAAATGTGAAGAATGGTTCAT
ATAAAATGGAGAGTCAAATGTTAACTGCTATCTCGATATTAGAAATGCTTAAATGTGCAATTTTCACATGGATTTGCACAATTTTATTGGAGTGAAGCTTTCACATATCACAAGACACACAACTGTAGTTGATGGTTTCTGTTCTATTGTGGTTGTCAAGAAATTTTGTTGGCAATTGTTCTACCTCTGCAGCATGCCCC
CAGTGAAATCCATTTCAAGTAGCCCCTCCACTGTTAATGTTTACTATTCTGGATAGTGCCAGCAGAAGATCTTGAATTTGAGACTTTCTACACAAAAAATATTATCCTGAAAGAAGGTATTCGTG