sp|Q8LGG8|USPAL_ARATH ----> Universal stress protein A-like protein

Intron 1 GTATAAGATT ... TGGTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.55	28	8.5	15
A2	27.27	24	4	14
A3	23.08	25	3	12
A4	22.89	32	13	6
A5	23.4	18	4	7
A6	20.37	349	59.5	119
A7	18.69	273	34.5	91
A8	19.57	222	31	77
A9	19.87	244	46	75
A10	17.68	149	26	38

>TRINITY_DN102951_c0_g1_i1 from sample A1 / Example spliced isoform

CTCCAACATACACGGTAGTCCACTAATATTAAAGTCTCCCGGAGCCCATGTTTGCTACAAATTCTCTGTTTGCACAGAAGTTCAGGCCTTGATTTCTGCACAATCCCATCCATTTGCATTCACAGGAGGAGGAAAATGGAAGAAAGTGGGAAGAAGAAAATAGTAGTAGGCGTGGACGATAGCGAGCAGAGCGTTTACGC
GTTACAATGGACTCTGGATTATCTGCTCGCTTCATTCCCTGCAGATCCGCCCTTCAAACTATTTCTGGTCCACGCTCGCCCTTCTGCAACCAGCGTTCTGCGCATGGCAGGCCCCGGCGTGGCGGAGGTCTTGCCTACTGTGGAGTTGGATCTGAAGAGAATCGGGGTCAGATCCCTCGAACATGCCAAAGGATTATGCC
AGAGCAGATCCATTGTTGATTTTGAAGCAGAGATGGTGGAGGGCGACGCCAGGAATGTCCTGTGCGAGGCCGTAGAGAGGCATGGCGCGGACATCTTAGCGGTGGGCAGCCATGGCTACGGAGCAATCAAGCGGGCAGTTTTGGGGAGTGTCAGCGACTACTGTGCGCATCATGCTAAGTGTACCGTCATGATTGTCAAG
AAGCCCAAGCACCACTGATCATAGGCAATTTATTCCATGCATCATGCTGTACTGTATTTATTTTTTATTTTGTGGCCCTTGCATCAAAGCTCCTGTGCTAGTAGATCTCAATGTTCAGAGATCAAAATTGGAATCATTCTGGGAATCCATAGCCATATGGGTTCCATATACTTTGTAACAGTTGTAATAGTGCATTGATC
TTCAATAGTATATTTAGAGTTTGTCAATTTTTCATAATAGTTGCTTTCAGTTTCATTTAAATGGTTTTAACTTTTCAGTTTCTTGAAATTATCTTTCAGTTTCTTTTAAATGGTTTTAACTTTTCAGAGCAATAATAGTTTTCAGAGCATTGAGTAGTTCTATTCAGATTTCATTCAATTAAATATTTTTGGGTATTTAT
CTATTTTATTTATTTATTTGTGTACAAGTTGGATTTCA

>TRINITY_DN102951_c0_g1_i3 from sample A1 / Example unspliced isoform:

CTCCAACATACACGGTAGTCCACTAATATTAAAGTCTCCCGGAGCCCATGTTTGCTACAAATTCTCTGTTTGCACAGAAGTTCAGGCCTTGATTTCTGCACAATCCCATCCATTTGCATTCACAGGAGGAGGAAAATGGAAGAAAGTGGGAAGAAGAAAATAGTAGTAGGCGTGGACGATAGCGAGCAGAGCGTTTACGC
GTTACAATGGACTCTGGATTATCTGCTCGCTTCATTCCCTGCAGATCCGCCCTTCAAACTATTTCTGGTCCACGCTCGCCCTTCTGCAACCAGCGTTCTGCGCATGGCAGGCCCCGGCGTGGCGGAGGTCTTGCCTACTGTGGAGTTGGATCTGAAGAGAATCGGGGTCAGATCCCTCGAACATGCCAAAGGATTATGCC
AGAGCAGATCCGTAAGTGCTCCCTTATTTTTCAGTCTTCGGCGCGGCCATGAGATTTCTGTTTTCTTTATTTTTTTTGGCCATCTGTGGTTGCAGATTGTTGATTTTGAAGCAGAGATGGTGGAGGGCGACGCCAGGAATGTCCTGTGCGAGGCCGTAGAGAGGCATGGCGCGGACATCTTAGCGGTGGGCAGCCATGGC
TACGGAGCAATCAAGCGgtataagattcttagttcttgatctttaatattagtcattagagaccaaatttgtgtgtatattactactacatagtgatggctggggattctgaggctgtgtgtatatgtcatggtgtgcagGGCAGTTTTGGGGAGTGTCAGCGACTACTGTGCGCATCATGCTAAGTGTACCGTCATGAT
TGTCAAGAAGCCCAAGCACCACTGATCATAGGCAATTTATTCCATGCATCATGCTGTACTGTATTTATTTTTTATTTTGTGGCCCTTGCATCAAAGCTCCTGTGCTAGTAGATCTCAATGTTCAGAGATCAAAATTGGAATCATTCTGGGAATCCATAGCCATATGGGTTCCATATACTTTGTAACAGTTGTAATAGTGC
ATTGATCTTCAATAGTATATTTAGAGTTTGTCAATTTTTCATAATAGTTGCTTTCAGTTTCATTTAAATGGTTTTAACTTTTCAGTTTCTTGAAATTATCTTTCAGTTTCTTTTAAATGGTTTTAACTTTTCAGAGCAATAATAGTTTTCAGAGCATTGAGTAGTTCTATTCAGATTTCATTCAATTAAATATTTTTGGG
TATTTATCTATTTTATTTATTTATTTGTGTACAAGTTGGATTTCA

Intron 2 GTATAAGATT ... TGGTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.88	28	2	15
A2	36.84	24	0	14
A3	32.43	25	0	12
A4	15.79	32	0	6
A5	28	18	0	7
A6	25.43	349	0	119
A7	25	273	0	91
A8	25.75	222	0	77
A9	23.51	244	0	75
A10	20.32	149	0	38

>TRINITY_DN34033_c0_g1_i2 from sample A9 / Example spliced isoform

CCCCAATTCTCGATCTCATTTTTCTTTTAAAGGTGACCTTTTTTGATCCCAGGGATTCGATCCCGTGACGTCGTTACAGGCGTGGCGGAGGTCTTGCCTACTGTGGAGTTGGATCTGAAGAGAATCGGGGTCAGATCCCTCGAACATGCCAAAGGATTATGCCAGAGCAGATCCGTAAGTGCTCCCTTATTTTTCAGTCT
TCGGCGCGGCCATGAGATTTCTGTTTTCTTTATTTTTTTTGGCCATTTGTGGTTGCAGATTGTTGATTTTGAAGCAGAGATGGTGGAGGGCGACGCCAGGAATGTCCTGTGCGAGGCCGTAGAGAGGCATGGCGCGGACATCTTAGCGGTGGGCAGCCATGGCTACGGAGCAATCAAGCGGGCAGTTTTGGGGAGTGTCA
GCGACTACTGTGCGCATCATGCTAAGTGTACCGTCATGATTGTCAAGAAGCCCAAGCACCACTGATCATAGGCAATTTATTCCATGCATCATGCTGTACTGTATTTATTTTTTATTTTGTGGCCCT

>TRINITY_DN34033_c0_g1_i3 from sample A9 / Example unspliced isoform:

ATGGCTACGGAGCAATCAAGCGgtataagatttttagttcttgatctttaatattagtcattagagaccaaatttgtgtgtatatactactacatagtgatggttggggattctgaggctgtgtgtatatgtcatggtgtgcagGGCAGTTTTGGGGAGTGTCAGCGACTACTGTGCGCATCATGCTAAGTGTACCGTCA
TGATTGTCAAGAAGCCCAAGCACCACTGATCATAGGCAATTTATTCCATGCATCATGCTGTACTGTATTTATTTTTTATTTTGTGGCCCTTGCATCAAAGCTCCTGTGCTAGTAGATCTCAATGTTCAGAGATCAAAATTGGAATCATTCTGGGAATCCATAGCCATATGGGTTCCATATACTTTGTAACAGTTGTAATA
GTGCATTGATCTTCAATAGTATATTTAGAGTTTGTCAATTTTTCATAATAGTTGCTTTCAGAC

Intron 3 GTAAGCAAAA ... GTCGTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20	16	4	1
A2	25	18	6	7
A3	17.65	14	3	2
A4	30.56	25	11	7
A5	11.11	8	1	2
A6	17.54	141	30	14.5
A7	21.56	131	36	12.5
A8	23.16	73	22	10.5
A9	15.63	108	20	13.5
A10	16.67	95	19	6.5

>TRINITY_DN40663_c0_g1_i3 from sample A7 / Example spliced isoform

CTATCCTTACGCGTGCTTAATACGCCGATTTGATTGAATTGGATCGATTCGAGAATTTTTGTACTTATTCGGAAGTGCTCCAACATACACGGTAGTCCACTAATATTAAAGTCTCCCGGAGCCCATGTTTGCTACAAATTCTCTGTTTGCACAGAAGTTCAGGCCTTGATTTCTGCACAATCCCATCCATTTGCATTCAC
AGGAGGAGGAAAATGGAAGAAAGTGGGAAGAAGAAAATAGTAGTAGGCGTGGACGATAGCGAGCAGAGCGTTTACGCGTTACAATGGACTCTGGATTATCTGCTCGCTTCATTCCCTGCAGATCCGCCCTTCAAACTATTTCTGGTCCACGCTCGCCCTTCTGCAACCAGCGTTCTGCGCATGGCAGGCCCCGGCGTGGC
GGAGGTCTTGCCTACTGTGGAGTTGGATCTGAAGAGAATCGGGGTCAGATCCCTCGAACATGCCAAAGGATTATGCCAGAGCAGATCCATTGTTGATTTTGAAGCAGAGATGGTGGAGGGCGACGCCAGGAATGTCCTGTGCGAGGCCGTAGAGAGGCATGGCGCGGACATCTTAGCGGTGGGCAGCCATGGCTACGGAG
CAATCAAGCGGGCAGTTTTGGGGAGTGTCAGCGACTACTGTGCGCATCATGCTAAGTGTACCGTCATGATTGTCAAGAAGCCCAAGCACCACTGATCATAGGCAATTTATTCCATGCATCATGCTGTACTGTATTTATTTTTTATTTTGTGGCCCTGCATCAAAGCTCCTGTGCTAGTATGATCTCAATGTTCAGAGATC
AAAATTGGAATCATTCTGGGAATCCATAGCCATATGGGTTCCATATACTTTGTAACAGTTGTAATAGTGCATTGATCTTCAATAGTATATTTAGAGTTTGTCAATTTTTCATAATAGTTGCTTTCAGTTTCATTTAAATGGTTTTAACTTTTCAGTTTCTTGAAATTATCTTTCAGTTTCTTTTAAATGGTTTTAACTTT
TCAGAG

>TRINITY_DN40663_c0_g1_i2 from sample A7 / Example unspliced isoform:

CTATCCTTACGCGTGCTTAATACGCCGATTTGATTGAATTGGATCGATTCGAGAATTTTTGTACTTATTCGGAAGTGCTCCAACATACACGGTAGTCCACTAATATTAAAGTCTCCCGGAGCCCATGTTTGCTACAAATTCTCTGTTTGCACAGAAGTTCAGGCCTTGATTTCTGCACAATCCCATCCATTTGCATTCAC
AGGAGGAGGAAAATGGAAGAAAGTGGGAAGAAGAAAATAGTAGTAGGCGTGGACGATAGCGAGCAGAGCGTTTACGCGTTACAATGGACTCTGGATTATCTGCTCGCTTCATTCCCTGCAGATCCGCCCTTCAAACTATTTCTGGTCCACGCTCGCCCTTCTGCAACCAGCGTTCTGCGCATGGCAGGCCCCGgtaagca
aaaaccccaattctcgatctcatttttcttttaaaggtgaccttttttgatcccagggattcgatcccgtgacgtcgttacagGCGTGGCGGAGGTCTTGCCTACTGTGGAGTTGGATCTGAAGAGAATCGGGGTCAGATCCCTCGAACATGCCAAAGGATTATGCCAGAGCAGATCCATTGTTGATTTTGAAGCAGAGA
TGGTGGAGGGCGACGCCAGGAATGTCCTGTGCGAGGCCGTAGAGAGGCATGGCGCGGACATCTTAGCGGTGGGCAGCCATGGCTACGGAGCAATCAAGCGGGCAGTTTTGGGGAGTGTCAGCGACTACTGTGCGCATCATGCTAAGTGTACCGTCATGATTGTCAAGAAGCCCAAGCACCACTGATCATAGGCAATTTAT
TCCATGCATCATGCTGTACTGTATTTATTTTTTATTTTGTGGCCCTGCATCAAAGCTCCTGTGCTAGTATGATCTCAATGTTCAGAGATCAAAATTGGAATCATTCTGGGAATCCATAGCCATATGGGTTCCATATACTTTGTAACAGTTGTAATAGTGCATTGATCTTCAATAGTATATTTAGAGTTTGTCAATTTTTC
ATAATAGTTGCTTTCAGTTTCATTTAAATGGTTTTAACTTTTCAGTTTCTTGAAATTATCTTTCAGTTTCTTTTAAATGGTTTTAACTTTTCAGAG

Intron 4 GTAAGTGCTC ... GTGGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.13	14	2.5	9
A2	11.76	15	6	2
A3	9.09	10	3	1
A4	33.33	30	4	15
A5	33.33	12	3	6
A6	23.27	136	12.5	41.3
A7	12.26	93	10	13
A8	21.05	90	13	24
A9	27.52	108	16.5	41
A10	28.89	64	6	26

>TRINITY_DN102951_c0_g1_i1 from sample A1 / Example spliced isoform

CTCCAACATACACGGTAGTCCACTAATATTAAAGTCTCCCGGAGCCCATGTTTGCTACAAATTCTCTGTTTGCACAGAAGTTCAGGCCTTGATTTCTGCACAATCCCATCCATTTGCATTCACAGGAGGAGGAAAATGGAAGAAAGTGGGAAGAAGAAAATAGTAGTAGGCGTGGACGATAGCGAGCAGAGCGTTTACGC
GTTACAATGGACTCTGGATTATCTGCTCGCTTCATTCCCTGCAGATCCGCCCTTCAAACTATTTCTGGTCCACGCTCGCCCTTCTGCAACCAGCGTTCTGCGCATGGCAGGCCCCGGCGTGGCGGAGGTCTTGCCTACTGTGGAGTTGGATCTGAAGAGAATCGGGGTCAGATCCCTCGAACATGCCAAAGGATTATGCC
AGAGCAGATCCATTGTTGATTTTGAAGCAGAGATGGTGGAGGGCGACGCCAGGAATGTCCTGTGCGAGGCCGTAGAGAGGCATGGCGCGGACATCTTAGCGGTGGGCAGCCATGGCTACGGAGCAATCAAGCGGGCAGTTTTGGGGAGTGTCAGCGACTACTGTGCGCATCATGCTAAGTGTACCGTCATGATTGTCAAG
AAGCCCAAGCACCACTGATCATAGGCAATTTATTCCATGCATCATGCTGTACTGTATTTATTTTTTATTTTGTGGCCCTTGCATCAAAGCTCCTGTGCTAGTAGATCTCAATGTTCAGAGATCAAAATTGGAATCATTCTGGGAATCCATAGCCATATGGGTTCCATATACTTTGTAACAGTTGTAATAGTGCATTGATC
TTCAATAGTATATTTAGAGTTTGTCAATTTTTCATAATAGTTGCTTTCAGTTTCATTTAAATGGTTTTAACTTTTCAGTTTCTTGAAATTATCTTTCAGTTTCTTTTAAATGGTTTTAACTTTTCAGAGCAATAATAGTTTTCAGAGCATTGAGTAGTTCTATTCAGATTTCATTCAATTAAATATTTTTGGGTATTTAT
CTATTTTATTTATTTATTTGTGTACAAGTTGGATTTCA

>TRINITY_DN102951_c0_g1_i3 from sample A1 / Example unspliced isoform:

CTCCAACATACACGGTAGTCCACTAATATTAAAGTCTCCCGGAGCCCATGTTTGCTACAAATTCTCTGTTTGCACAGAAGTTCAGGCCTTGATTTCTGCACAATCCCATCCATTTGCATTCACAGGAGGAGGAAAATGGAAGAAAGTGGGAAGAAGAAAATAGTAGTAGGCGTGGACGATAGCGAGCAGAGCGTTTACGC
GTTACAATGGACTCTGGATTATCTGCTCGCTTCATTCCCTGCAGATCCGCCCTTCAAACTATTTCTGGTCCACGCTCGCCCTTCTGCAACCAGCGTTCTGCGCATGGCAGGCCCCGGCGTGGCGGAGGTCTTGCCTACTGTGGAGTTGGATCTGAAGAGAATCGGGGTCAGATCCCTCGAACATGCCAAAGGATTATGCC
AGAGCAGATCCgtaagtgctcccttatttttcagtcttcggcgcggccatgagatttctgttttctttattttttttggccatctgtggttgcagATTGTTGATTTTGAAGCAGAGATGGTGGAGGGCGACGCCAGGAATGTCCTGTGCGAGGCCGTAGAGAGGCATGGCGCGGACATCTTAGCGGTGGGCAGCCATGGC
TACGGAGCAATCAAGCGGTATAAGATTCTTAGTTCTTGATCTTTAATATTAGTCATTAGAGACCAAATTTGTGTGTATATTACTACTACATAGTGATGGCTGGGGATTCTGAGGCTGTGTGTATATGTCATGGTGTGCAGGGCAGTTTTGGGGAGTGTCAGCGACTACTGTGCGCATCATGCTAAGTGTACCGTCATGAT
TGTCAAGAAGCCCAAGCACCACTGATCATAGGCAATTTATTCCATGCATCATGCTGTACTGTATTTATTTTTTATTTTGTGGCCCTTGCATCAAAGCTCCTGTGCTAGTAGATCTCAATGTTCAGAGATCAAAATTGGAATCATTCTGGGAATCCATAGCCATATGGGTTCCATATACTTTGTAACAGTTGTAATAGTGC
ATTGATCTTCAATAGTATATTTAGAGTTTGTCAATTTTTCATAATAGTTGCTTTCAGTTTCATTTAAATGGTTTTAACTTTTCAGTTTCTTGAAATTATCTTTCAGTTTCTTTTAAATGGTTTTAACTTTTCAGAGCAATAATAGTTTTCAGAGCATTGAGTAGTTCTATTCAGATTTCATTCAATTAAATATTTTTGGG
TATTTATCTATTTTATTTATTTATTTGTGTACAAGTTGGATTTCA