sp|P05621|H2B2_WHEAT ----> Histone H2B.2

Intron 1 GTAAGTCCTT ... CCATTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	51.85	7.4	8	2
A2	74.21	3.9	11	3
A3	83.18	1.4	7	3
A4	59.26	1.4	2	1
A5	61.69	2.4	3.9	1
A6	69.53	47.8	109	23
A7	64.37	31	56	14
A8	69.93	27.1	62.9	22
A9	61.93	30.8	50	15
A10	51.87	22.9	24	7

>TRINITY_DN44429_c0_g4_i3 from sample A6 / Example spliced isoform

CTGGAGTAGTGTGCGCACCAATCTGAGTTTGAATTTGTCTGTCGATCCCATTTCGGTTTGAGTTTTAGGAGAATTCGGTTGTGAAGAATGGCACCAAAGGCAGAGAAGAAGCCGGCGGAGAAGAAGCCCGCAGGCGGAGAGGAAGAGAGGAAGACTGTCGGAGAGAAGACTCCGGTTGGAAAGGCGGCGGCGGCAGCCGC
GGCGAAGGAGAAGGCTGCTCCTCCTGCTGCCTCCAAGGCAAAGAAGAAGATAACTAAAGAAGGAGGCGATAAAAAGAAGAAGAAGAGGGCAAAGAAGGGGGTGGAGACATACAAGATATATGTGTACAAGGTGCTGAAGCAGGTTCATCCTGACATTGGTATTTCCAGCAAGGCTATGTCCATCATGAACTCTTTTGTCA
ATGATATCTTTGAGAAAGTGGCGGCAGAGTCATCGAAGCTTACCCGCTATGGTAAGAGGGACACGCTTTCCTCCAGGGAGGTGCAGACTGCTGTTAAACTCGTTCTTCCGGGCGAGCTTTCTAAGCATGCTGTGTCCGAGGGGTATGGCGCTTTAACAGTAACATTTTGGGTGAAGGGCTGGAGGTTTCTCTCACTGATC
AGGTATTGAGGATGTTACCCACGATCTTGGAATCAATTCATGTACACTGCCTATTGTTGTGGAAATGCGAAACGCATTGGGAAGTATATTAAATGTGTTGTGTGTTCAGATTGAAGAAAGTGACAGTATAAGACAATATATGTGAATTTCTACCTTTGTGGGAAGTGTGATCGATATGTATCTACGTGTGAAGGACAGAT
TTATAGTTGCTGAAGTTTCTTCAATTATCTCTGGTGTAAATTAATTGAAGTAAGCAATATTTGATACCAGGATGAGAACCTTACCAAAATTTTATTATTAAAGGTTTGTTTTTCAAATCAATTGTCTCTGGTGTAAATTTATGGAAGTAAGCAATATTTGATACCAGGATGAGAACCTTACCAAAATTTTATTATTAAAG
GTTTGTTTTTCAAATCAATTGTCTCTGGTGTAAATTTATGGAAGTAAGCAATATTTGATACCA

>TRINITY_DN44429_c0_g4_i2 from sample A6 / Example unspliced isoform:

CTGGAGTAGTGTGCGCACCAATCTGAGTTTGAATTTGTCTGTCGATCCCATTTCGGTTTGAGTTTTAGGAGAATTCGGTTGTGAAGAATGGCACCAAAGGCAGAGAAGAAGCCGGCGGAGAAGAAGCCCGCAGGCGGAGAGGAAGAGAGGAAGACTGTCGGAGAGAAGACTCCGGTTGGAAAGGCGGCGGCGGCAGCCGC
GGCGAAGGAGAAGGCTGCTCCTCCTGCTGCCTCCAAGGCAAAGAAGAAGATAACTAAAGAAGGAGGCGATAAAAAGAAGAAGAAGAGGGCAAAGAAGGGGGTGGAGACATACAAGATATATGTGTACAAGGTGCTGAAGCAGGTTCATCCTGACATTGGTATTTCCAGCAAGGCTATGTCCATCATGAACTCTTTTGTCA
ATGATATCTTTGAGAAAGTGGCGGCAGAGTCATCGAAGCTTACCCGCTATGGTAAGAGGGACACGCTTTCCTCCAGGGAGGTGCAGACTGCTGTTAAACTCGTTCTTCCGGGCGAGCTTTCTAAGCATGCTGTGTCCGAGGGTACTAAGGCTGTCACTAAATTTACTTCCACTTGAGCTCAGCTCTTCTCTGCTCTGTGC
ATTTTGTGGATTTCTGCTTACGGTGTTTCTTGACACCACCTCTTCCTACAGAATTTTGGGCCCCTTTTTTCGTCTGCCGCTTTAACATTTTGGATGATAGTTTTCTTCGATGTGTTGTTGATTAGGGTATGGCGCTTTAACAGTAACATTTTGGGTGAAGGGCTGGAGGTTTCTCTCACTGATCAGGTATTGgtaagtcc
ttttctttttcccttgaagggcataccgaaaggtttttcaacttttgttattttcttgaaaagttctaccttcgaaggctatggcgaagggtatttctataggggccttttctcttaaagagctgttaaggtttgtacaagcgttgtggatgtaaaagttaataccaacagcagtcaatatctagtgaatttgaaggaat
attcctttttcgtgtgtgtattttcatcgggtttaaatgtttagctctattgtattgttctgttgtgtgagtggttccgtctactgaaactaaaagataaaagctttatgccattccagttagattattgtgctgtatgtagtgtaataaaacgcagatgcgttatttatttaaccaataagccgtctgtattgcactgg
aaattgaatactctggtacagacatgtaagtatctgtgcaagttttattaaaaagaacaaaaggaacctgcagattgcaactttcactattgttgtagtcttgtagccaattctggaatgtggaaagatagtggttttgttaatatgatactttctggcatattgagtagccaatatcttacttatgtatttctattttc
catttgtagAGGATGTTACCCACGATCTTGGAATCAATTCATGTACACTGCCTATTGTTGTGGAAATGCGAAACGCATTGGGAAGTATATTAAATGTGTTGTGTGTTCAGATTGAAGAAAGTGACAGTATAAGACAATATATGTGAATTTCTACCTTTGTGGGAAGTGTGATCGATATGTATCTACGTGTGAAGGACAGA
TTTATAGTTGCTGAAGTTTCTTCAATTATCTCTGGTGTAAATTAATTGAAGTAAGCAATATTTGATACCAGGATGAGAACCTTACCAAAATTTTATTATTAAAGGTTTGTTTTTCAAATCAATTGTCTCTGGTGTAAATTTATGGAAGTAAGCAATATTTGATACCAGGATGAGAACCTTACCAAAATTTTATTATTAAA
GGTTTGTTTTTCAAATCAATTGTCTCTGGTGTAAATTTATGGAAGTAAGCAATATTTGATACCA

Intron 2 GTACTAAGGC ... TGTTGATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.27	6	12	10.6
A2	59.06	4	5	6.6
A3	80.95	2	11	6
A4	81.01	1	6	2.6
A5	71.52	3	13	2.1
A6	63.29	45	102	53.4
A7	64.65	26	64	31.1
A8	75.36	16	63	35
A9	68.22	27.2	80.2	36.7
A10	66.81	17	42	26.4

>TRINITY_DN117261_c1_g2_i4 from sample A1 / Example spliced isoform

GTGCGCACCAATCTGAGTTTGAATTTGTCTGTCGATCCCATTTCGGTTTGAGTTTTAGGAGAATTCGGTTGTGAAGAATGGCACCAAAGGCAGAGAAGAAGCCGGCGGAGAAGAAGCCCGCAGGCGGAGAGGAAGAGAGGAAGACTGTCGGAGAGAAGACTCCGGTTGGAAAGGCGGCGGCGGCAGCCGCGGCGAAGGAG
AAGGCTGCTCCTCCTGCTGCCTCCAAGGCAAAGAAGAAGATAACTAAAGAAGGAGGCGATAAAAAGAAGAAGAAGAGGGCAAAGAAGGGGGTGGAGACATACAAGATATATGTGTACAAGGTTCTGAAGCAGGTTCATCCTGACATTGGTATTTCCAGCAAGGCTATGTCCATCATGAACTCTTTTGTCAATGATATCTT
TGAGAAAGTGGCGGCAGAGTCATCGAAGCTTACCCGCTATGGTAAGAGGGACACGCTTTCCTCCAGGGAGGTGCAGACTGCTGTTAAACTCGTTCTTCCGGGCGAGCTTTCTAAGCATGCTGTGTCCGAGGGGTATGGCGCTTTAACAGTAACATTTTGGGTGAAGGGCTGGAGGTTTCTCTCACTGATCAGGTATTGAG
GATGTTACCCACGATCTTGGAATCAATTCATGTACACTGCCTATTGTTGTGGAAATGCGAAACGCATTGGGAAGTATATTAAATGTGTTGTGTGTTCAGATTGAAGAAAGTGAC

>TRINITY_DN117261_c1_g2_i9 from sample A1 / Example unspliced isoform:

GTGCGCACCAATCTGAGTTTGAATTTGTCTGTCGATCCCATTTCGGTTTGAGTTTTAGGAGAATTCGGTTGTGAAGAATGGCACCAAAGGCAGAGAAGAAGCCGGCGGAGAAGAAGCCCGCAGGCGGAGAGGAAGAGAGGAAGACTGTCGGAGAGAAGACTCCGGTTGGAAAGGCGGCGGCGGCAGCCGCGGCGAAGGAG
AAGGCTGCTCCTCCTGCTGCCTCCAAGGCAAAGAAGAAGATAACTAAAGAAGGAGGCGATAAAAAGAAGAAGAAGAGGGCAAAGAAGGGGGTGGAGACATACAAGATATATGTGTACAAGGTTCTGAAGCAGGTTCATCCTGACATTGGTATTTCCAGCAAGGCTATGTCCATCATGAACTCTTTTGTCAATGATATCTT
TGAGAAAGTGGCGGCAGAGTCATCGAAGCTTACCCGCTATGGTAAGAGGGACACGCTTTCCTCCAGGGAGGTGCAGACTGCTGTTAAACTCGTTCTTCCGGGCGAGCTTTCTAAGCATGCTGTGTCCGAGGgtactaaggctgtcactaaatttacttccacttgagctcagctcttctctgctctgtgcattttgtgga
tttctgcttacggtgtttcttgacaccacctcttcctacagaattttgggccccttttttcgtctgccgctttaacattttggatgatagttttcttcgatgtgttgttgattagGGTATGGCGCTTTAACAGTAACATTTTGGGTGAAGGGCTGGAGGTTTCTCTCACTGATCAGGTATTGGTAAGTCCTTTTCTTTTT
CCCTTGAAGGGCATACCGAAAGGTTTTTCAACTTTTGTTATTTTCTTGAAAAGTTCTACCTTCGAAGGCTATGGCGAAGGGTATTTCTATAGGGGCCTTTTCTCTTAAAGAGCTGTTAAGGTTTGTACAAGCGTTGTGGATGTAAAAGTTAATACCAACAGCAGTCAATATCTAGTGAATTTGAAGGAATATTCCTTTTT
CGTGTGTGTATTTTCATCGGGTTTAAATGTTTAGCTCTATTGTATTGTTCTGTTGTGTGAGTGGTTCCGTCTACTGAAACTAAAAGATAAAAGCTTTATGCCATTCCAGTTAGATTATTGTGCTGTATGTAGTGTAATAAAACGCAGATGCGTTATTTATTTAACCAATAAGCCGTCTGTATTGCACTGGAAATTGAATA
CTCTGGTACAGACATGTAAGTATCTGTGCAAGTTTTATTAAAAAGAACAAAAG

Intron 3 GTATTGGTAA ... CCATTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.14	6	8	2
A2	66.67	6	12	3
A3	70	3	7	3
A4	50	3	3	1
A5	50	3	3	1
A6	68.13	51	109	23
A7	62.37	35	58	14
A8	64.29	35	63	22
A9	59.04	34	49	15
A10	46.67	32	28	7

>TRINITY_DN45122_c2_g1_i4 from sample A7 / Example spliced isoform

GGACGGTTCAGATCGCTGCAAAGTCCACGATCCGTGTCTCATCTCACAACCAATCAGAAGCGCCACAGCTCATATATATGGAGGCGATCACTTGCAGACAAAGCCATCAGCTCTGGAGTAGTGTGCGCACCAATCTGAGTTTGAATTTGTCTGTCGATCCCATTTCGGTTTGAGTTTTAGGAGAATTCGGTTGTGAAGAA
TGGCACCAAAGGCAGAGAAGAAGCCGGCGGAGAAGAAGCCCGCAGGCGGAGAGGAAGAGAGGAAGACTGTCGGAGAGAAGACTCCGGTTGGAAAGGCGGCGGCGGCAGCCGCGGCGAAGGAGAAGGCTGCTCCTCCTGCTGCCTCCAAGGCAAAGAAGAAGATAACTAAAGAAGGAGGCGATAAAAAGAAGAAGAAGAGG
GCAAAGAAGGGGGTGGAGACATACAAGATATATGTGTACAAGGTGCTGAAGCAGGTTCATCCTGACATTGGTATTTCCAGCAAGGCTATGTCCATCATGAACTCTTTTGTCAATGATATCTTTGAGAAAGTGGCGGCAGAGTCATCGAAGCTTACCCGCTATGGTAAGAGGGACACGCTTTCCTCCAGGGAGGTGCAGAC
TGCTGTTAAACTCGTTCTTCCGGGCGAGCTTTCTAAGCATGCTGTGTCCGAGGGTACTAAGGCTGTCACTAAATTTACTTCCACTTGAGCTCAGCTCTTCTCTGCTCTGTGCATTTTGTGGATTTCTGCTTACGGTGTTTCTTGACACCACCTCTTCCTACAGAATTTTGGGCCCCTTTTTTCGTCTGCCGCTTTAACAT
TTTGGATGATAGTTTTCTTCGATGTGTTGTTGATTAGGGTATGGCGCTTTAACAGTAATATTTTGGGTGAAGGGCTGGAGGTTTCTCTCACTGATCAGAGGATGTTACCCACGATCTTGGAATCAATTCATGTACACTGCCTATTGTTGTGGAAATGCGAAACGCATTGGGAAGTATATTAAATGTGTTGTGTGTTCAGA
TTGAAGAAAGTGACAGTATAAGACAATATATGTGAATTTCTACCTTTGTGGGAAGTGTGATCGATATGTATCTACGTGTGAAGGACAGATTTATAGTTGCTGAAGTTTCTTCAATTATCTCTGGTGTAAATTAATTGAAGTAAGCAATATTTGATACCAGGATGAGAACCTTACCAAAATTTTATTATTAAAGGTTTGTT
TTTCAAATCAATTGTCTCTGGTGTAAATTTATGGAAGTAAGCAATATTTGATACCAGGATGAGAACCTTACCAAAATTTTATTATTAAAGGTTTGTTTTTCAAATCAATTGTCTCTGGTGTAAATTTATGGAAGTAAGCAATATTTGATACCA

>TRINITY_DN45122_c2_g1_i6 from sample A7 / Example unspliced isoform:

TACTAAGGCTGTCACTAAATTTACTTCCACTTGAGCTCAGCTCTTCTCTGCTCTGTGCATTTTGTGGATTTCTGCTTACGGTGTTTCTTGACACCACCTCTTCCTACAGAATTTTGGGCCCCTTTTTTCGTCTGCCGCTTTAACATTTTGGATGATAGTTTTCTTCGATGTGTTGTTGATTAGGGTATGGCGCTTTAACA
GTAATATTTTGGGTGAAGGGCTGGAGGTTTCTCTCACTGATCAGgtattggtaagtccttttctttttcccttgaagggcataccgaaaggtttttcaacttttgttattttcttgaaaagttctaccttcgaaggctatggcgaagggtatttctataggggccttttctcttaaagagctgttaaggtttgtacaagc
gttgtggatgtaaaagttaataccaacagcagtcaatatctagtgaatttgaaggaatattcctttttcgtgtgtgtattttcatcgggtttaaatgtttagctctattgtattgttctgttgtgtgagtggttccgtctactgaaactaaaagataaaagctttatgccattccagttagattattgtgctgtatgtag
tgtaataaaacgcagatgcgttatttatttaaccaataagccgtctgtattgcactggaaattgaatactctggtacagacatgtaagtatctgtgcaagttttattaaaaagaacaaaaggaacctgcagattgcaactttcactattgttgtagtcttgtagccaattctggaatgtggaaagatagtggttttgtta
atatgatactttctggcatattgagtagccaatatcttacttatgtatttctattttccatttgtagAGGATGTTACCCACGATCTTGGAATCAATTCATGTACACTGCCTATTGTTGTGGAAATGCGAAACGCATTGGGAAGTATATTAAATGTGTTGTGTGTTCAGATTGAAGAAAGTGACAGTATAAGACAATATAT
GTGAATTTCTACCTTTGTGGGAAGTGTGATCGATATGTATCTACGTGTGAAGGACAGATTTATAGTTGCTGAAGTTTCTTCAATTATCTCTGGTGTAAATTAATTGAAGTAAGCAATATTTGATACCAGGATGAGAACCTTACCAAAATTTTATTATTAAAGGTTTGTTTTTCAAATCAATTGTCTCTGGTGTAAATTTA
TGGAAGTAAGCAATATTTGATACCAGGATGAGAACCTTACCAAAATTTTATTATTAAAGGTTTGTTTTTCAAATCAATTGTCTCTGGTGTAAATTTATGGAAGTAAGCAATATTTGATACCA