NA ----> NA

Intron 1 GCTTTAAAAC ... TATCGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.36	91	100	2
A2	51.44	101	107	4
A3	53.8	85	99	6
A4	51.06	69	72	0
A5	49.52	53	52	1
A6	51.54	362.5	385.5	8
A7	51.44	540	572	16
A8	52.47	423	467	12
A9	51.82	530	570	10
A10	52.93	474	533	9

>TRINITY_DN44189_c0_g1_i8 from sample A7 / Example spliced isoform

GAAGGATTAAGCGTGGTTGACGTGTGAAGGTGGTGGTGGAGGCTTTTTCAAATTTAACCGTAATGGAAGAGGAGCGTGTGGAGAAGCGTGGGATCGAGCCATTCATCAATCGATCACCGCTGGAGAGAACGAAAAAGCCGTTGCATCCCCGATCGATTCGTCGATTATATATCACTACGGTTCATTCAGCCAGGCCTCCA
CTTCCTGTCCGCCTTTGCCTTCTTGAGAATGGAGAGGAGGAGAGAAAACTATACGCCATATGGAGAATGGGAGGGAGGGATTTTTACATGAATGATCGGACGCAGACGAATTAACTTTCAGATTTCTAATGGTAAAATTATGGAAGAGGACAGCAGAAGCAGATGGCATGGGATGAACAGCAGCAGCAGATGGCGATGGA
TGCTGCTGAACTACAGATGCTGCTGAACTACAAGATTGATGATCTAACAGGCAAATATATCGCCGAGGATCAAAAAAACAACATATCAGATTTATCAAGGGAAATGTCAGCAAACATCGGCAATATATCAACGAACATAAAAAATACAAAAATATTGAACTCAATCAATATTTTTCCGATTTTGGCGATATTTTTCGATT
TTTGCCAATATCTTCAAAATATTGTGATATTTACAAACTACAATTTTTCTAGCGATAATATCACTGAATTGAGAGCATCGCGATATATCGCCAATGTTTGTAATACTGCTTTAAAACACATTAGGGCAAGTTATAATTCTGCAAAAACTTCTGTGCAAGTAAACATTTCAGATTTCACTGCTAAATTCATTTACATGGTC
CGTTCACCAAAATTCTAGAATTTTGAAATTTTCTGTTAATAATATCCTTTGTTACCATTAGGAGCAGCT

>TRINITY_DN44189_c0_g1_i1 from sample A7 / Example unspliced isoform:

GAAGGATTAAGCGTGGTTGACGTGTGAAGGTGGTGGTGGAGGCTTTTTCAAATTTAACCGTAATGGAAGAGGAGCGTGTGGAGAAGCGTGGGATCGAGCCATTCATCAATCGATCACCGCTGGAGAGAACGAAAAAGCCGTTGCATCCCCGATCGATTCGTCGATTATATATCACTACGGTTCATTCAGCCAGGCCTCCA
CTTCCTGTCCGCCTTTGCCTTCTTGAGAATGGAGAGGAGGAGAGAAAACTATACGCCATATGGAGAATGGGAGGGAGGGATTTTTACATGAATGATCGGACGCAGACGAATTAACTTTCAGATTTCTAATGGTAAAATTATGGAAGAGGACAGCAGAAGCAGATGGCATGGGATGAACAGCAGCAGCAGATGGCGATGGA
TGCTGCTGAACTACAGATGCTGCTGAACTACAAGATTGATGATCTAACAGgctttaaaacaaagttacaaatatcgtgtagGCAAATATATCGCCGAGGATCAAAAAAACAACATATCAGATTTATCAAGGGAAATGTCAGCAAACATCGGCAATATATCAACGAACATAAAAAATACAAAAATATTGAACTCAATCAAT
ATTTTTCCGATTTTGGCGATATTTTTCGATTTTTGCCAATATCTTCAAAATATTGTGATATTTACAAACTACAATTTTTCTAGCGATAATATCACTGAATTGAGAGCATCGCGATATATCGCCAATGTTTGTAATACTGCTTTAAAACACATTAGGGCAAGTTATAATTCTGCAAAAACTTCTGTGCAAGTAAACATTTC
AGATTTCACTGCTAAATTCATTTACATGGTCCGTTCACCAAAATTCTAGAATTTTGAAATTTTCTGTTAATAATATCCTTTGTTACCATTAGGAGCAGCT

Intron 2 GTTGTTAGCA ... ATGGTTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.79	8	9	18
A2	62.96	5	6	11
A3	92	1	8	15
A4	63.64	4	4	10
A5	100	0	4	3
A6	63.83	17	25	35
A7	73.58	14	31	47
A8	69.23	14	33	30
A9	63.04	17	31	27
A10	67.86	9	18	20

>TRINITY_DN39230_c1_g2_i8 from sample A6 / Example spliced isoform

CATTTACAAAGCAAACTTAAGCGTGGTGAAGGATTAAGCGTGGTTGACGTGTGAAGGTGGTGGTGGAGGCTTTTTCAAATTTAACCGTAATGGAAGAGGAGCGTGTGGAGAAGCGTGGGATCGAGCCATTCATCAATCGATCACCGCTGGAGAGAACGAAAAAGCCGTTGCATCCCCGATCGATTCGTCGATTATATATC
ACTACGGTTCATTCAGCCAGGCCTCCACTTCCTGTCCGCCTTTGCCTTCTTGAGAATGGAGAGGAGGAGAGAAAACTATACGCCATATGGAGAATGGGAGGGAGGGATTTTTACATGAATGATCGGACGCAGACGGATTAACTTTCAGATTTCTAATGGTAAAATTATGGAAGAGGACAGCAGAAGCAGATGGCATGGGA
TGAACAGCAGCAGCAGATGGCGATGGATGCTGCTGAACTACAGATGCTGCTGAACTACAAGATTGATGATCTAACAGGTTGGAACACTTTGAAAACTAATGTGCCCAGTTGGTAGAGTTGAGGATTTGAGAACTATTGCAAGGACGGGGTAGATAACTTCATCAACCGCCAGAACCGAGTAAGGCACTTTATGATGGAGC
ACTAAACTTGGGAGCTTATTTCCTTCACATGTTGGACTATTTTCACTTCCTAAGAGTTCATTCTACCTTGGATTGAGCACCTACACGAATTTTGGAGCCTAATTATGAGGAGGCGCCCTTGCTTTTTGCACCTGTTAGTTGGAGATTTGACCATATTGCTAGATTGTAGACACATTCTAGAAGTGCGCCCTCTCTTCAAG
GAGTGGTCCCATGGTATTAAAGAGCACCCTCTCTTCGTGAGCCCATTATGTATTATTTATCTATATTTTGACCATTTAAATCTGGTGCACCCTCCCTCATTGAGGAAAAGGTTAGGATTTGGCATTATTTTATTGTTTTTGACCATATCTTGGTGATTCCCTAGATCAAATCTAGGATTTTAGACATTTATTTCTTTTTT
ATTGTGAATGTAGTTGACTCCTTCTCCTATAAAACGAACTCACTCCTCACTTGTCATTTGTTGTTGTTGATGTTGGAGAAAGAATACAATCATTTCATTTCC

>TRINITY_DN39230_c1_g2_i2 from sample A6 / Example unspliced isoform:

CATTTACAAAGCAAACTTAAGCGTGGTGAAGGATTAAGCGTGGTTGACGTGTGAAGGTGGTGGTGGAGGCTTTTTCAAATTTAACCGTAATGGAAGAGGAGCGTGTGGAGAAGCGTGGGATCGAGCCATTCATCAATCGATCACCGCTGGAGAGAACGAAAAAGCCGTTGCATCCCCGATCGATTCGTCGATTATATATC
ACTACGGTTCATTCAGCCAGGCCTCCACTTCCTGTCCGCCTTTGCCTTCTTGAGAATGGAGAGGAGGAGAGAAAACTATACGCCATATGGAGAATGGGAGGGAGGGATTTTTACATGAATGATCGGACGCAGACGGATTAACTTTCAGATTTCTAATGGTAAAATTATGGAAGAGGACAGCAGAAGCAGATGGCATGGGA
TGAACAGCAGCAGCAGATGGCGATGGATGCTGCTGAACTACAGATGCTGCTGAACTACAAGATTGATGATCTAACAGGCACTTTATGATGGAGCACTAAACTTGGGAGCTTATTTCCTTCACATGTTGGACTATTTTCACTTCCTAAGAGTTCATTCTACCTTGGATTGAGCACCTACACGAATTTTGGAGCCTAATTAT
GAGgttgttagcatgtttgactacctcaagagcagtttgggaccacatgtgtggcagtttcaatttctcccatggttttggaacacagtttgatgatctttgctcatggttttagGAGGCGCCCTTGCTTTTTGCACCTGTTAGTTGGAGATTTGACCATATTGCTAGATTGTAGACACATTCTAGAAGTGCGCCCTCTC
TTCAAGGAGTGGTCCCATGGTATTAAAGAGCACCCTCTCTTCGTGAGCCCATTATGTATTATTTATCTATATTTTGACCATTTAAATCTGGTGCACCCTCCCTCATTGAGGAAAAGGTTAGGATTTGGCATTATTTTATTGTTTTTGACCATATCTTGGTGATTCCCTAGATCAAATCTAGGATTTTAGACATTTATTTC
TTTTTTATTGTGAATGTAGTTGACTCCTTCTCCTATAAAACGAACTCACTCCTCACTTGTCATTTGTTGTTGTTGATGTTGGAGAAAGAATACAATCATTTCATTTCC

Intron 3 GTTGGAACAC ... GTCTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.06	231	136	41
A2	39.15	230	148	21
A3	36.41	241	138	28
A4	39.25	178	115	21
A5	43.66	80	62	13
A6	38.43	886	553	93
A7	36.92	1324	775	140
A8	37.76	1060	643	158
A9	37.02	1283	754	155
A10	36.95	1169	685	124

>TRINITY_DN133041_c4_g2_i7 from sample A1 / Example spliced isoform

GAAGGATTAAGCGTGGTTGACGTGTGAAGGTGGTGGTGGAGGCTTTTTCAAATTTAACCGTAATGGAAGAGGAGCGTGTGGAGAAGCGTGGGATCGAGCCATTCATCAATCGATCACCGCTGGAGAGAACGAAAAAGCCGTTGCATCCCCGATCGATTCGTCGATTATATATCACTACGGTTCATTCAGCCAGGCCTCCA
CTTCCTGTCCGCCTTTGCCTTCTTGAGAATGGAGAGGAGGAGAGAAAACTATACGCCATATGGAGAATGGGAGGGAGGGATTTTTACATGAATGATCGGACGCAGACGGATTAACTTTCAGATTTCTAATGGTAAAATTATGGAAGAGGACAGCAGAAGCAGATGGCATGGGATGAACAGCAGCAGCAGATGGCGATGGA
TGCTGCTGAACTACAGATGCTGCTGAACTACAAGATTGATGATCTAACAGCTTGGGAAACAAGAAAACATGGACAATTCGGGGAACTATGGGAGCTACCCTGCACCAGATATCAGGGAAATTCAAAAGATTTTTCACAGGCATGAAGACCTCATCAAAGACAACAGAACTTCCATATTCTCTGTCACGGTTAAGGTTCAT
GCCAATGAGGAGGCCAACAGCAGTAAGCATGCAGCAATGATGAACTCCATACTTCAGTAAAGAGAGCAAATTCAGGCTCTTGAAGCTGAATATCTCTCCATTAAAACTCAGTTTACTAATCTGAACAGGGGTGCTCTCTCAGCAGCATGAAATTACAAAATGTCAAATGATTATGTTGTTGGAGAAATAAAGGAAGAGGA
AATGATCCATCAAGCTCAAGAAGATGAGGATATGGAGCCTAAAAACTCTCAGCCCTCCAATATCAGTCAGAACGAAGCTTTCGGTACTCATGCTTTCCATAAGAATAGTATCTCTCTATGTATTGCAAAATCTGAAAACAGTTATGATTTTAGAAATCTTTTTTTAAATCTGTACTAGCAGAAATCTAGGTATATGGATA
TCCTCTGCGAAGGATACTGTCACCTTGCTGGCATTATCTCTTTTTCATTTAGGTATTTTGTAGAATTTCAGATGCAGGGACTACTTCTTTTTGTATTATTAAGCTGACCAGCGAGGCTGCAGCATTTTCTCAATACAAATATCAACCATTTCTGTTAAAAATAATAAATAATAACATCATTAGAGAGCATCTAACTTTTT
CTATTACAATCTTTACTGTCTAAAATTACATCGGGAAATGTACTCA

>TRINITY_DN133041_c4_g2_i6 from sample A1 / Example unspliced isoform:

GAAGGATTAAGCGTGGTTGACGTGTGAAGGTGGTGGTGGAGGCTTTTTCAAATTTAACCGTAATGGAAGAGGAGCGTGTGGAGAAGCGTGGGATCGAGCCATTCATCAATCGATCACCGCTGGAGAGAACGAAAAAGCCGTTGCATCCCCGATCGATTCGTCGATTATATATCACTACGGTTCATTCAGCCAGGCCTCCA
CTTCCTGTCCGCCTTTGCCTTCTTGAGAATGGAGAGGAGGAGAGAAAACTATACGCCATATGGAGAATGGGAGGGAGGGATTTTTACATGAATGATCGGACGCAGACGGATTAACTTTCAGATTTCTAATGGTAAAATTATGGAAGAGGACAGCAGAAGCAGATGGCATGGGATGAACAGCAGCAGCAGATGGCGATGGA
TGCTGCTGAACTACAGATGCTGCTGAACTACAAGATTGATGATCTAACAGgttggaacactttgaaaactaatgtgcccagttggtagagttgaggatttgagaactattgcaaggacggggtagataacttcatcaaccgccagaaccgatccacttcgaatcaatccagcttccggggcatttggttttgattctatt
tatctggtctcttcagCTTGGGAAACAAGAAAACATGGACAATTCGGGGAACTATGGGAGCTACCCTGCACCAGATATCAGGGAAATTCAAAAGATTTTTCACAGGCATGAAGACCTCATCAAAGACAACAGAACTTCCATATTCTCTGTCACGGTTAAGGTTCATGCCAATGAGGAGGCCAACAGCAGTAAGCATGCAG
CAATGATGAACTCCATACTTCAGTAAAGAGAGCAAATTCAGGCTCTTGAAGCTGAATATCTCTCCATTAAAACTCAGTTTACTAATCTGAACAGGGGTGCTCTCTCAGCAGCATGAAATTACAAAATGTCAAATGATTATGTTGTTGGAGAAATAAAGGAAGAGGAAATGATCCATCAAGCTCAAGAAGATGAGGATATG
GAGCCTAAAAACTCTCAGCCCTCCAATATCAGTCAGAACGAAGCTTTCGGTACTCATGCTTTCCATAAGAATAGTATCTCTCTATGTATTGCAAAATCTGAAAACAGTTATGATTTTAGAAATCTTTTTTTAAATCTGTACTAGCAGAAATCTAGGTATATGGATATCCTCTGCGAAGGATACTGTCACCTTGCTGGCAT
TATCTCTTTTTCATTTAGGTATTTTGTAGAATTTCAGATGCAGGGACTACTTCTTTTTGTATTATTAAGCTGACCAGCGAGGCTGCAGCATTTTCTCAATACAAATATCAACCATTTCTGTTAAAAATAATAAATAATAACATCATTAGAGAGCATCTAACTTTTTCTATTACAATCTTTACTGTCTAAAATTACATCGG
GAAATGTACTCA

Intron 4 GTTGGAACAC ... ACCGAGTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.26	91	138	8
A2	59.6	101	149	6
A3	62.05	85	139	12
A4	62.7	69	116	6
A5	53.91	53	62	6
A6	60.84	363	564	42
A7	59.25	540	785	49
A8	60.61	423	651	51
A9	59.04	530	764	47
A10	59.28	474	690	37

>TRINITY_DN40837_c1_g1_i12 from sample A9 / Example spliced isoform

CTTCCATTTACAAAGCAAACTTAAGCGTGGTGAAGGATTAAGCGTGGTTGACGTGTGAAGGTGGTGGTGGAGGCTTTTTCAAATTTAACCGTAATGGAAGAGGAGCGTGTGGAGAAGCGTGGGATCGAGCCATTCATCAATCGATCACCGCTGGAGAGAACGAAAAAGCCGTTGCATCCCCGATCGATTCGTCGATTATA
TATCACTACGGTTCATTCAGCCAGGCCTCCACTTCCTGTCCGCCTTTGCCTTCTTGAGAATGGAGAGGAGGAGAGAAAACTATACGCCATATGGAGAATGGGAGGGAGGGATTTTTACATGAATGATCGGACGCAGACGGATTAACTTTCAGATTTCTAATGGTAAAATTATGGAAGAGGACAGCAGAAGCAGATGGCAT
GGGATGAACAGCAGCAGCAGATGGCGATGGATGCTGCTGAACTACAGATGCTGCTGAACTACAAGATTGATGATCTAACAGGCAAATATATCGCCGAGGATCAAAAAAACAACATATCAGATTTATCAAGGGAAATGTCAGCAAACATCGGCAATATATCAACGAACATAAAAAATACAAAAATATTGAACTCAATCAAT
ATTTTTCCGATTTTGGCGATATTTTTCGATTTTTGCCAATATCTTCAAAATATTGTGATATTTACAAACTACAATTTTTCTAGCGATAATATCACTGAATTGAGAGCATCGCGATATATCGCCAATGTTTGTAATACTGCTTTAAAACACATTAGGGCAAGTTATAATTCTGCAAAAACTTCTGTGCAAGTAAACATTTC
AGATTTCACTGCTAAATTCATTTACATGGTCCGTTCACCAAAATTCTAGAATTTTGAAATTTTCTGTTAATAATATCCTTTGTTACCATTAGGAGCAGCAAGATAATGAATTATGGTTTGAATTGCATGG

>TRINITY_DN40837_c1_g1_i16 from sample A9 / Example unspliced isoform:

CTTCCATTTACAAAGCAAACTTAAGCGTGGTGAAGGATTAAGCGTGGTTGACGTGTGAAGGTGGTGGTGGAGGCTTTTTCAAATTTAACCGTAATGGAAGAGGAGCGTGTGGAGAAGCGTGGGATCGAGCCATTCATCAATCGATCACCGCTGGAGAGAACGAAAAAGCCGTTGCATCCCCGATCGATTCGTCGATTATA
TATCACTACGGTTCATTCAGCCAGGCCTCCACTTCCTGTCCGCCTTTGCCTTCTTGAGAATGGAGAGGAGGAGAGAAAACTATACGCCATATGGAGAATGGGAGGGAGGGATTTTTACATGAATGATCGGACGCAGACGGATTAACTTTCAGATTTCTAATGGTAAAATTATGGAAGAGGACAGCAGAAGCAGATGGCAT
GGGATGAACAGCAGCAGCAGATGGCGATGGATGCTGCTGAACTACAGATGCTGCTGAACTACAAGATTGATGATCTAACAGgttggaacactttgaaaactaatgtgcccagttggtagagttgaggatttgagaactattgcaaagacggggtagataacttcatcaaccgccagaaccgagtaagGCAAATATATCGC
CGAGGATCAAAAAAACAACATATCAGATTTATCAAGGGAAATGTCAGCAAACATCGGCAATATATCAACGAACATAAAAAATACAAAAATATTGAACTCAATCAATATTTTTCCGATTTTGGCGATATTTTTCGATTTTTGCCAATATCTTCAAAATATTGTGATATTTACAAACTACAATTTTTCTAGCGATAATATCA
CTGAATTGAGAGCATCGCGATATATCGCCAATGTTTGTAATACTGCTTTAAAACACATTAGGGCAAGTTATAATTCTGCAAAAACTTCTGTGCAAGTAAACATTTCAGATTTCACTGCTAAATTCATTTACATGGTCCGTTCACCAAAATTCTAGAATTTTGAAATTTTCTGTTAATAATATCCTTTGTTACCATTAGGA
GCAGCAAGATAATGAATTATGGTTTGAATTGCATGG