sp|Q5K5B6|RH57_ORYSJ ----> DEAD-box ATP-dependent RNA helicase 57

Intron 1 GTTGGGAAAT ... TAGATTTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55	7.5	12	5.5
A2	57.27	5	11	2.5
A3	62.28	3.5	8	3.5
A4	50	8.5	12	5
A5	61.91	2.5	6	2
A6	52.98	46.5	64	40.5
A7	49.34	26.5	27	23
A8	55.09	34	46	35.5
A9	58.66	25.5	48	24.5
A10	55.84	23.5	38	20.5

>TRINITY_DN134185_c0_g2_i4 from sample A1 / Example spliced isoform

GAGAGAGAAGCAAAATTGAATGTTGCCCTACAAAATCTGGTTGAAACTGGTGATTCGCCCCGTATTTTGGGTATGAAGGCCACGAAATATTGAATTCACCCCATTTTCTCAGTACTGTATCCTCCAAATGGCGAGTTCGCCCCATTTTATCAATAATGTAGCCTCCAAATGGCGAGTTCGCCTCGATTGGAGGATGATGC
AGCTGGTTCGTGGACTTTATCAGCTAAATCGCCCTCGATTTGAGTTGGGAAATGGGCAGTGCCAGTCTCACCAAATAAGTGACCACGCCCTCACTTTCAGGTGAATTTGAAGGTGAAGGTAGTTTCAGTTGCATTACCTGGCACTGCAGCTGCTCAAGCGAAAAATGTGTCAACAACCTAGGTCATCTTAGACAAGTATG
TTTTCATCCTCTATACATACGAAGTTCCAACATTGTACATTGTGAATCACTGGAATGACAAA

>TRINITY_DN134185_c0_g2_i1 from sample A1 / Example unspliced isoform:

GAGAGAGAAGCAAAATTGAATGTTGCCCTACAAAATCTGGTTGAAACTGGTGATTCGCCCCGTATTTTGGGTATGAAGGCCACGAAATATTGAATTCACCCCATTTTCTCAGTACTGTATCCTCCAAATGGCGAGTTCGCCCCATTTTATCAATAATGTAGCCTCCAAATGGCGAGTTCGCCTCGATTGGAGGATGATGC
AGCTGGTTCGTGGACTTTATCAGCTAAATCGCCCTCGATTTGAGTTGGGAAATGGGCAGTGCCAGTCTCACCAAATAAGTGACCACGCCCTCACTTTCAGgttgggaaatgggcagtctccccgagtagacattcagttaccacgccctagatttgagGTGAATTTGAAGGTGAAGGTAGTTTCAGTTGCATTACCTGGC
ACTGCAGCTGCTCAAGCGAAAAATGTGTCAACAACCTAGGTCATCTTAGACAAG

Intron 2 GTTAGTTTTC ... AATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.3	27	11	27
A2	31.03	30	6	21
A3	37.21	27	9	23
A4	30.43	16	1	13
A5	26.03	27	9	10
A6	20.99	32	4	13
A7	19.35	25	4	8
A8	18.42	31	4	10
A9	21.05	30	3	13
A10	40	6	4	4

>TRINITY_DN46263_c0_g1_i19 from sample A8 / Example spliced isoform

GGGGTTTCAAACATTTGATGGGCGAATTTGTAACATGAAAAGAGTTTTTTTAAATTTAATAATTTAGCCCCAAATTTATTTGACCCTCGTCAATGGGGAATCGAATCGCCAAGGCTAGATAGGCACGAGAGAGAAGCAAAATTGAATTCGCCCTATCAAATCTGGTTGAAACTGATGATTCGCCCCGTAATTTGGGTATG
AAAGCCACGAAATATTGAATTCGCCCCATTTTTCAGTAATGTAGCTTGCAAATGGCAAGTTCGCCCCGTTTTATTAGTTATGTATCCTCCAAATGGCGAGTTCGCCGCGTTTTTTCAGTTATGTATCCTCCAAATGGCGAGTTCGCCCCGTTTTATCAATTATGTAGCCTCCAAATGGCGAGCTTGCCCCTATTGGACGA
TGATGCAGTTGGTTCGTGGATTCTGTCAGCTAAATCGCCCTCGATTTGAGTTGGGAAATGGGCAGTTCAGTTTATCCCTATATAATGTGGCAAGAGTCAATAGAAACCCACATCATCAGATCTTTCATCTTTGCATGAAGCTTGGTGGTGTTTTGCATAGTTTCCTTGGGGTTTGCAGTCTTTAGAGAGGTGAATTTGAA
AGTGAAGGTAGTTTCGGTTGCATTACCTATCACTGCAACTGCTCAAGCGAAAAATCCGTCAACAACCTGGGTCATCTTAGACAAGTATGTTTCCATCCTCTATACATATGAAGTTCCGACATTGTAAACTGTGAATCACTGGAATGACAAATTAGTATTTAATTTGGGGTTTTACAATGTCTTCCCGAATCCCCATTGAA
GACTGAAGTTTTAGGATTGTTATGAAGTGAGCTTCATGGTGTTGTCCTGTTTCTATGATTTGTCCTCCTGTAGATAGCTAAAGAAAGAATAAAATATTGCATGGAATGACTGCACTACAATCTCATGGTTATAAAGAGTTTTGGCCCCAAAATTCTTTGTA

>TRINITY_DN46263_c0_g1_i16 from sample A8 / Example unspliced isoform:

GGGTTTGCAGTCTTTAGAGAGgttagttttcttcaaaccaaatactgtgcagactgcagtggacattatgctatggatgcattcattgttgtattttctatagtctttaaactattgttgaagctttgaaatttaattttgcagGTGAATTTGAAAGTGAAGGTAGTTTCGGTTGCATTACCTATCACTGCAACTGCTCA
AGCGAAAAATCCGTCAACAACCTGGGTCATCTTAGACAAGT

Intron 3 GTGAGTTCGC ... CTTTAGAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.97	5	20	8
A2	82.97	6	29	9.5
A3	84.52	4	22	8
A4	88.28	5	36.5	14
A5	75.97	5	16	12
A6	70.3	18	43	18
A7	71.21	13.5	33	12
A8	88.31	4.5	34	20
A9	79.64	14	55	10
A10	84.79	8	46	5

>TRINITY_DN45661_c0_g3_i1 from sample A7 / Example spliced isoform

GGGAAATCAAATCGCCAAGGCCAGATAGGCACGAGAGAGAAGCAAAATTGAATTCGTCCTACCAAATCTGGTTGAAACTGGTGATTCATCCCGTAGTTTGGGTATGAAGGCCACGAAATATTGAATTCGCCCCATTTTTCCAGTAATGTAGCTTGCAAATGGTGAATTTGAAGGTGAAGGTAGTTTCAGTTGCATTACCT
GGCACTGCAGCTGCTCAAGCGAAAAATCTGTCAACAACTTGGGTCATCTTAGACAAGTTGAAAATGCCAATATTTCTGAAGTTGTGCGCATAATTGATTCAGGCTTCATTCCTGTGGTTGCATAAGAAGAATCAAATTTTCATTTTCATCTATTCCAATAACGAGCAACATCAAAACATACTACCACCAATTGGCACTAC
AAAGAGCCTACAAATTTTTTCCATGTAACATAGCAAGTGATTCTATTACATGAGGAGTGCCTCCAAGGAAATTATGATATGGTTTCTAAACTACAAATTGCATGCTCAACTGTGCAACCTACTTCTCAATGCCAAAGGGTAATCTATGATGAAGTTTTTTCTACATGAGATCATTCATAGTAAAAGAATAGAAATAAGTT
GTCAATTTTTTGTTGATTAACATACATGTTTCCTATCATGTGCTCAAG

>TRINITY_DN45661_c0_g3_i4 from sample A7 / Example unspliced isoform:

GGGAAATCAAATCGCCAAGGCCAGATAGGCACGAGAGAGAAGCAAAATTGAATTCGTCCTACCAAATCTGGTTGAAACTGGTGATTCATCCCGTAGTTTGGGTATGAAGGCCACGAAATATTGAATTCGCCCCATTTTTCCAGTAATGTAGCTTGCAAATGgtgagttcgccccgttttatcagttatgtagcctccaaa
tggcaagttcgccccattttatcagttatgtagcttccaaatggcgattttgccccgattggaggatgatgcagctggttcgtggactctgtcagctaaatcgcccttgatttgagttgggaaatgggcagttcaaacttcaatttatccctatataatgtggcaagagtcaatagaaacccacatcatcagatctttcg
tctttgcatgaagcttggttttttttatagtggatgtctctgggtttgttcaaatttccttggggttttcattctttagagagGTGAATTTGAAGGTGAAGGTAGTTTCAGTTGCATTACCTGGCACTGCAGCTGCTCAAGCGAAAAATCTGTCAACAACTTGGGTCATCTTAGACAAGTACGTTTCCATCCTCTATACA
TATGAAGTTCCAACATTGTAAACTGTGAATCACTGGAATGACAAATTCTAGTATTTAATTTGGGGTTTTACAATGTCTTCCCGAATCCCCATTGAAGTTTTAGGATTGTTATGAAGTGAGCTTCATGGTGTTGTCCTATTTCTATGATTTGTCCTCCTATAGATAGCTAAAGAAAGAAGAAAATATTGCATGGAATGACT
GCGTTACAATCTCATGGTTATAAAGAGTTTTGGCCACAAAATTCTTTGTATAAACCTTATAGATCTACTTTTTAATAGGCGGTTTGTGCCAGAAGACCTTTAGCTACAAGGCCATGATTAATTTAGCTATAAACTAAACTTTATGAAGCCTGCAATACAAACTTATTAATTTTGGGTAATACTAAAAGGATAAACTTGAA
GGAAAACATAGTAGAATCTAAAGAAATATATAAGGAAATGCCAACAGAAATAGAGTGGCTTTAGAAACCTGAGACACCATATACATATATTACTTAGTTTCCTATAACCAAAGAATGGAATTTGTTTTGAGGTTGAAACCAAATGGAGGTGTAGTAGAGAAACAGGGCACACTGAGCATGCAGCCTAAGGGATATATGTA
AGATTGGGTCTCTTTATGGTCACCTAAGTTTCCTGTCTCCTTGTGCATCTTAAGGTTTTGGGGCTTTTGTAGTGTTGTATGAGGCAGTGTTGTGATTCCTACCTTGCAAAACATGAGATTCTAGAAGTTGACAGTTACAAACAACTGCCGTACATAGAGAATACTGGACTCCAACTAGATCTGCA