sp|Q93WI9|HD3A_ORYSJ ----> Protein HEADING DATE 3A

Intron 1 GTATCATGGT ... ACAATATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.79	884	160	249
A2	17.32	924	163	224
A3	15.66	1034	168	216
A4	16.87	707	134	153
A5	22.52	535	121	190
A6	15.46	10029	1501	2167
A7	17.73	9076	1561	2350
A8	17.02	9441	1503	2369
A9	16.48	10350	1615	2468
A10	16.19	8265	1227	1967

>TRINITY_DN44718_c0_g2_i6 from sample A6 / Example spliced isoform

CTCTCATCAAATTCAGTTCCACTAGAAATTATTTAAATACTGCTTCCCAGTTTTCATTCCCTTCACAAGCGACCTCCATCGTTGAATTCTTCCTGATTCGGGATTGTGAAGCGAATGGAAGCGGTGACTAAGCTGCTTAGAGAAGCGGGAGTTCTCGGAAGCATTGTCGACAATGAGGCAGAGGGAGTGCAAGATGCAGA
TGCTGTTTTCGATGGTGTTGTCGAGGATTTCGGTCCACTAGAGTTTCAGTTGGATGTAATCTACGGCGATAAGCGAATCAGCCCAGGGGAGACTGTCGCCCCTTTTGAAGTGCAACACAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCCTGGAAACCTTCCGGCACCCCACATTTATAACTG
TAGTTATGACGGATCTTGATTCTGATGGGGGCGAGTATTTGCACTGGTTATCTACGGCCGCCCAACAAGCACCAGTGATGCAATGGCAGGATCTGGTAACCTATGAACCTCCAACGGGACGACTCATGGGTACCCGCAAGATAGTTTGCATAGTCTACAAATCGAATGAAGCTTTACCCAATATCATTGTACCTGAGTCG
CGGAACAATTTCAGTACCAGATTTTGGGCATACGATAATGAAATTCACGATGATCCGC

>TRINITY_DN44718_c0_g2_i8 from sample A6 / Example unspliced isoform:

CTCTCATCAAATTCAGTTCCACTAGAAATTATTTAAATACTGCTTCCCAGTTTTCATTCCCTTCACAAGCGACCTCCATCGTTGAATTCTTCCTGATTCGGGATTGTGAAGCGAATGGAAGCGGTGACTAAGCTGCTTAGAGAAGCGGGAGTTCTCGGAAGCATTGTCGACAATGAGGCAGAGGGAGTGCAAGATGCAGA
TGCTGTTTTCGATGGTGTTGTCGAGGATTTCGGTCCACTAGAGTTTCAGTTGGATGTAATCTACGGCGATAAGCGAATCAGCCCAGGGGAGACTGTCGCCCCTTTTGAAGTGCAACACAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCCTGGAAACCTTCCGGCACCCCACATTTATAACTG
TAGTTATGACGGATCTTGATTCTGATGGGGGCGAGTATTTGCACTGGTTATCTACGGCCGCCCAACAAGCACCAGgtatcatggttgtgtttctttagttgagctagaaatcgatcgatccaatgcgttccgtagtgttaaccgtcgtggatgttcgtttctttgtgctacaatattagTGATGCAATGGCAGGATCTGG
TAACCTATGAACCTCCAACGGGACGACTCATGGGTACCCGCAAGATAGTTTGCATAGTCTACAAATCGAATGAAGCTTTACCCAATATCATTGTA

Intron 2 GTAAGATTAA ... TTTTGTTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50.56	310	317	5
A2	50.45	331	337	10
A3	50.85	405	419	8
A4	50.08	314	315	0
A5	51.62	239	255	8
A6	50.21	2609	2631	63
A7	50.57	2568	2627	69
A8	51.11	1954	2043	83
A9	50.65	2727	2799	75
A10	50.35	2341	2374	63

>TRINITY_DN120409_c0_g4_i7 from sample A2 / Example spliced isoform

CTTATATGTATTTATGATGTCATATAATCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATAT
TGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCCGGGGACACTGTCGCCCCTGTTGA
AGTGCAACAGAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGTGGAAACCTTCCGGCACCCCACATTTATAACTGTCCTTGAAATCGATCGATCGAATGCGTTCCGTAGTGTTAACCGTGGTGGATGTTCGTTTCTTTGTGCTGCAATATTAGTGATGCAATGGCAG

>TRINITY_DN120409_c0_g4_i1 from sample A2 / Example unspliced isoform:

CTTATATGTATTTATGATGTCATATAATCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATAT
TGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCCGGGGACACTGTCGCCCCTGTTGA
AGTGCAACAGAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGTGGAAACCTTCCGGCACCCCACATTTATAACTGTCgtaagattaaatcatgtttcctccttatttttcccaataatttttttttatctatttggcaaatttaaatccatattattatctgttcttaaatccataactaggt
tatttggcggtgagcatttgcactggtatgctatcagtttcctcaagtcacatcaaattattgattgtatagcacaggaatctattaataacaaattattgattgtatcatggttgtgtttcttttgttgagCTTGAAATCGATCGATCGAATGCGTTCCGTAGTGTTAACCGTGGTGGATGTTCGTTTCTTTGTGCTGC
AATATTAGTGATGCAATGGCAG

Intron 3 GTAAGATTAA ... CCATAACTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.24	1465	284	6
A2	16.79	1526	308	4
A3	16.25	1959	380	7
A4	17.33	1326	278	4
A5	17.06	1099	226	8
A6	13.81	15797	2531	36
A7	14.27	15086	2511	44
A8	13.59	12365	1945	61
A9	14	16558	2696	52
A10	14	14014	2281	25

>TRINITY_DN118003_c2_g4_i3 from sample A3 / Example spliced isoform

ATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATATTGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTT
GTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCTCGGGGACACTGTCGCCCCTGTTGAAGTGCAACACAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGT
GGAAACCTTCCGGCACCCCACATTTATAACTGTCGTTATGACGGATCCTGACGCTGTTGGCGGTGAGCATTTGCACTGGTTATCTACGGCAACACAACAAGCACCAGTGATGCAATGGCAGGATATTGTAACCTATGAACCTCCGAGGGGACGACTCATGGGTACCCGCAAGATCGTTTGCACAGTCTACAAATCGAATG
AAGCTTTACCCAATATCATTGTACCTGAGTCGCGGAACAATTTCAGTACCAGATTTTGGGCATACAATAGTGTCATTAACGATGATCCGCTGGCTGTTACATTTTTCAAAATCATCGGTCCGGTGGAGAATGGTTAATATTCAAGAATTACCCGCATCTAACAGAGGATGAGAATGGTTATCAAGAATTATCCCCATGTA
ACGGTCATGTACGTTGAGTGCCGTTTTAATATCGGTGAACTGGCTACACGTTCTATGACATAAAACCATGATAGAATTTTCACAGCCATGAAAGTCTCAGTCGTGCTTATTATCATCTCAATATTTTTAATCAGGAGGTTCGAAAGGTTTTCAAAATTTCTCAACGTAATTTTATCTGTCATTTCTTAATAGTTATTCTA
CAGAGATGTGTTACATGTAAGACTGTGTTTAATCGTTGTAAAATATATATGATTTTTTTAGGTCGGCAAGTTTGGTATCTTGAAATTTCAATGAGATACGTTTTAAAGCTTACGGATATTGAGTATCTCAGGC

>TRINITY_DN118003_c2_g4_i5 from sample A3 / Example unspliced isoform:

ATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATATTGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTT
GTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCCGGGGACACTGTCGCCCCTGTTGAAGTGCAACACAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGTG
GAAACCTTCCGGCACCCCACATTTATAACTGTCgtaagattaaatcatgtttcctccttatttttcccaataatttttttttatctatttggcaaatttaaatccatattattatctgttcttaaatccataactagGTTATGACGGATCCTGACGCTG

Intron 4 GTTATGACGG ... CAAGCACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.47	349	692	113
A2	67.19	379	776	104
A3	68.59	457	998	95
A4	65.8	369	710	79
A5	69.36	258	584	32
A6	71.86	2912	7438	723
A7	71.6	2888	7281	739
A8	70.53	2282	5461	733
A9	71.81	3154	8036	826
A10	71.47	2762	6919	679

>TRINITY_DN120409_c0_g4_i6 from sample A2 / Example spliced isoform

CTTATATGTATTTATGATGTCATATAATCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATAT
TGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCCGGGGACACTGTCGCCCCTGTTGA
AGTGCAACAGAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGTGGAAACCTTCCGGCACCCCACATTTATAACTGTCTGATGCAATGGCAGGATCTTGTAAGCTATGAACCTCCAAGGGGACGACTCATGGGTACCCGCAAGATAGTTTACATAGTCTACAAATCGAATGAAGCTTTACCCAA
TATC

>TRINITY_DN120409_c0_g4_i5 from sample A2 / Example unspliced isoform:

CTTATATGTATTTATGATGTCATATAATCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATAT
TGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCTCGGGGACACTGTCGCCCCTGTTG
AAGTGCAACAGAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGTGGAAACCTTCCGGCACCCCACATTTATAACTGTCgttatgacggatcctgacgctgttggcggttatctacggtcgccctacaagcaccagTGATGCAATGGCAGGATCTTGTAAGCTATGAACCTCCAAGGGGACGAC
TCATGGGTACCCGCAAGATAGTTTACATAGTCTACAAATCGAATGAAGCTTTACCCAATATC

Intron 5 GTTATCTACG ... GAAGCACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.97	481	1201	1040
A2	67.34	541	1174	1057
A3	68.07	633	1516	1183
A4	65.91	464	976	818
A5	68.97	324	802	638
A6	67.21	6120	13431	11653
A7	66.73	5750	12491	10577
A8	68.42	5085	11292	10738
A9	67.1	6439	14033	12231
A10	66.91	5191	11476	9518

>TRINITY_DN47833_c1_g1_i3 from sample A7 / Example spliced isoform

TGAAGAGTTCGTATTACTGTTTGAACGATTTAAATATCTTGATCTCGCGAAAAATATAACTTAAATGTATTTATGATGTCATATATTCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTTTCCATCTCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGA
CAGTTTTCATTCCTTTGACAAGCGACCTCCATCATTGAATTCTTCCTGATTTCGGATATTGTCAAGAGAATGGAGGTGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGGCAGAGGGAGTGGTAGGCGCAGATGATGTTTTCGATGCTCTTGTCGACCATTTCGGATGTAATCTACG
GCGATAAACGAATAAGCCCAGGGGAAAGTGTCGCCCCTGTTGAAGTGCAACACAGGCCTGATTGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCCTGGAAACCTTCCGGCACCCCACATTTATAACTGTAGTTATGACGGATCCTGACGCTGTTGGCGGCGAGCATTTGCACTGTGATGCAATGGCAGGATTT
TGTAACCTATGAACCTCCAAGGGGACGACTCATGGGTACCCGCAAGATAGTTTACATAGTCTACAAATCGAATGAAGCTTTACCCAATATCATTGTACCTGAGTCTCGGAACAATTTCAGTAGCAGATTTTGGGCATATCATAG

>TRINITY_DN47833_c1_g1_i6 from sample A7 / Example unspliced isoform:

TGAAGAGTTCGTATTACTGTTTGAACGATTTAAATATCTTGATCTCGCGAAAAATATAACTTAAATGTATTTATGATGTCATATATTCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTTTCCATCTCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGA
CAGTTTTCATTCCTTTGACAAGCGACCTCCATCATTGAATTCTTCCTGATTTCGGATATTGTCAAGAGAATGGAGGTGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGGCAGAGGGAGTGGTAGGCGCAGATGATGTTTTCGATGCTCTTGTCGACCATTTCGGATGTAATCTACG
GCGATAAACGAATAAGCCCAGGGGAAAGTGTCGCCCCTGTTGAAGTGCAACACAGGCCTGATTGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCCTGGAAACCTTCCGGCACCCCACATTTATAACTGTAGTTATGACGGATCCTGACGCTGTTGGCGGCGAGCATTTGCACTGgttatctacggcaacacaa
gaagcaccagTGATGCAATGGCAGGATTTTGTAACCTATGAACCTCCAAGGGGACGACTCATGGGTACCCGCAAGATAGTTTACATAGTCTACAAATCGAATGAAGCTTTACCCAATATCATTGTACCTGAGTCTCGGAACAATTTCAGTAGCAGATTTTGGGCATATCATAG

Intron 6 GTTATGACGG ... CAAGCACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.84	419.5	1441.5	903
A2	72.26	463	1500	884.5
A3	73.83	524.5	1925	1017
A4	70.47	424	1310	702
A5	73.78	289.5	1077	545.5
A6	77.73	3665.5	15442.5	9807.5
A7	76.8	3647.5	14801	9013
A8	77.97	3055.5	12128.5	9057
A9	77.17	3969	16165	10293
A10	76.89	3319.5	13746	8150.5

>TRINITY_DN120409_c0_g4_i6 from sample A2 / Example spliced isoform

CTTATATGTATTTATGATGTCATATAATCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATAT
TGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCCGGGGACACTGTCGCCCCTGTTGA
AGTGCAACAGAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGTGGAAACCTTCCGGCACCCCACATTTATAACTGTCTGATGCAATGGCAGGATCTTGTAAGCTATGAACCTCCAAGGGGACGACTCATGGGTACCCGCAAGATAGTTTACATAGTCTACAAATCGAATGAAGCTTTACCCAA
TATC

>TRINITY_DN120409_c0_g4_i3 from sample A2 / Example unspliced isoform:

CTTATATGTATTTATGATGTCATATAATCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATAT
TGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCTCGGGGACACTGTCGCCCCTGTTG
AAGTGCAACAGAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGTGGAAACCTTCCGGCACCCCACATTTATAACTGTCgttatgacggatcctgacgctgttggcggtgagcatttgcactggttatctacggtcgccctacaagcaccagTGATGCAATGGCAGGATCTTGTAAGCTATGAA
CCTCCAAGGGGACGACTCATGGGTACCCGCAAGATAGTTTACATAGTCTACAAATCGAATGAAGCTTTACCCAATATC

Intron 7 GTAGCATGGT ... GCAATATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.43	1098	369	303
A2	22.06	1097	338	283
A3	20.38	1229	351	278
A4	22.56	853	270	227
A5	25.26	673	227	228
A6	20.89	12132	3609	2797
A7	22.87	11011	3579	2950
A8	22.32	11269	3481	2994
A9	21.78	12674	3916	3144
A10	22.15	9881	3121	2501

>TRINITY_DN118003_c2_g4_i2 from sample A3 / Example spliced isoform

GCAGATGATGTTTTCGATGGCGTTGTCGACGATTTCGGTCCCCTAGAGTTTCAGTTGGATGTAATCTACGGCGATAAACGAATAAGCCCAGGGGAGACTGTCGCCCCTGTTGAAGTGCAACACAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCTGAGATCCTCGAAACCTTCCGGGACCCCACATTTAT
AACTGTGGTTATGACGGATCCTGACGCTGTTGGCGGCGAGCATTTGCACTGGTTATCTACGGCAACACAACAAGCACCAGTGATGCAATGGCAGGATATTGTAACCTATGAACCTCCGAGGGGACGACTCATGGGTACCCGCAAGATCGTTTGCACAGTCTACAAATCGAATGAAGCTTTACCCAATATCATTGTACCTG
AGTCGCGGAACAATTTCAGTACCAGATTTTGGGCATACAATAGTGTCATTAACGATGATCCGCTGGCTGTTACATTTTTCAAAATCATCGGTCCGGTGGAGAATGGTTATTCAAGAATTACCCGCATCTAACAGAGGATGAGAATGGTTATCAAGAATTATCCCCATGTAACGGTCATGTACGTTGAGTGCCGTTTTAAT
ATCGGTGAACTGGCTACACGTTCTATGACATAAAACCAGAATAGAATTTTCACAGCCATGAAAGTCTCAGTCGTGCTTATTATCATCTCAATATTTTTAAT

>TRINITY_DN118003_c2_g4_i7 from sample A3 / Example unspliced isoform:

GCAGATGATGTTTTCGATGGCGTTGTCGACGATTTCGGTCCCCTAGAGTTTCAGTTGGATGTAATCTACGGCGATAAACGAATAAGCCCAGGGGAGACTGTCGCCCCTGTTGAAGTGCAACACAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCTGAGATCCTCGAAACCTTCCGGGACCCCACATTTAT
AACTGTGGTTATGACGGATCCTGACGCTGTTGGCGGCGAGCATTTGCACTGGTTATCTACGGCAACACAACAAGCACCAGgtagcatggttgtgtttcttttgttgagcttgaaatcgatcgatcgaatgcgttccgtagtgttaaccgtggtggatgttcgtttctttgtgctgcaatattagTGATGCAATGGCAGGA
TCTTGTAAGCTATGAACCTCCAAGGGGACGACTCATGGGTACCCGCAAGATCGTTTGCACAGTCTACAAATCGAATGAAGCTTTACCCAATATCATTGTA

Intron 8 GTAAGATTAA ... GCAATATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.6	349	317	2
A2	47.07	379	337	7
A3	47.83	457	419	1
A4	46.05	369	315	0
A5	49.71	258	255	1
A6	47.47	2912	2631	38
A7	47.63	2888	2627	38
A8	47.24	2282	2043	51
A9	47.02	3154	2799	54
A10	46.22	2762	2374	40

>TRINITY_DN120409_c0_g4_i6 from sample A2 / Example spliced isoform

CTTATATGTATTTATGATGTCATATAATCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATAT
TGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCCGGGGACACTGTCGCCCCTGTTGA
AGTGCAACAGAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGTGGAAACCTTCCGGCACCCCACATTTATAACTGTCTGATGCAATGGCAGGATCTTGTAAGCTATGAACCTCCAAGGGGACGACTCATGGGTACCCGCAAGATAGTTTACATAGTCTACAAATCGAATGAAGCTTTACCCAA
TATC

>TRINITY_DN120409_c0_g4_i1 from sample A2 / Example unspliced isoform:

CTTATATGTATTTATGATGTCATATAATCTCGGCTCCTCCGTTTTTCAAGATCAAGATTCCATTAAGAGCGGTCGAGATCGCAAGCTCTCCATATCTCTCATCAATTCAGTTCTACTGGAAAAGATTTAAATACCGCTTGCCAGTTTTCATTCCTTTCACAAGCGACCTCCATCATTGAATTCTTCCTGATTCCGGATAT
TGCCAAGACAATGGAGGCGGTGAATCAGCTGCTTAGAGAAGCGGGAGTTCTCGGAGGCTTTGTCGACAGCGAGACAGAGGGAGTGGTAGGGGCAGATGATGTTTTCGATGCCGTTGTCGACGAGTTCAGTCCAGAATTTCAGTTGGATGTAATCTACGGCCATAAACGAATAAGCCCGGGGACACTGTCGCCCCTGTTGA
AGTGCAACAGAGGCCTGATCGTTTTGAGTTCCTTCGCGATCCTTCTCATCCCGAGATCGTGGAAACCTTCCGGCACCCCACATTTATAACTGTCgtaagattaaatcatgtttcctccttatttttcccaataatttttttttatctatttggcaaatttaaatccatattattatctgttcttaaatccataactaggt
tatttggcggtgagcatttgcactggtatgctatcagtttcctcaagtcacatcaaattattgattgtatagcacaggaatctattaataacaaattattgattgtatcatggttgtgtttcttttgttgagcttgaaatcgatcgatcgaatgcgttccgtagtgttaaccgtggtggatgttcgtttctttgtgctgc
aatattagTGATGCAATGGCAG