sp|P29344|RR1_SPIOL ----> Small ribosomal subunit protein bS1c

Intron 1 GTAAGTTTCT ... CTCATGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.54	28	31	8
A2	55.17	26	32	17
A3	53.06	23	26	26
A4	40.74	16	11	14
A5	53.57	13	15	4
A6	51.24	235	247	30
A7	52.93	217	244	32
A8	50.91	244	253	34
A9	49.68	237	234	42
A10	52.94	152	171	31

>TRINITY_DN45068_c0_g2_i3 from sample A6 / Example spliced isoform

GGAAGATTAATTGGCTTTCAAAAGAATGATACCCAAAGATATCCTTGGTGGTATTTTCAGCTTTCTTATCCAAAACCCACCTACGATAGTCCACTCTCCTCTCATTCTCATTTCACATTTCAGACGCTCACTGCTGCTGATTGTCTTACGATCATGGCAGCAGTAACCCAGCAATTGGTCCTCTCAGGGGTCTATTTCAC
ATGCTCCCGCAGTAGCAAACTGTGCTGCTCAAGGCCGAGGCAATGGCAGTCACAGTACCAGTCCCTGTCTCACCCCCAGGGGAAGAACCAGAAGAAGAACAAGAGCAGAGGCGTTGTGTGTGCAGTGGCGATTTCGAATGCAAAGACGCGAGAGAGGGAATCCCTCATGGAGGAAATCGAAGAGAGATTGAGGAAAATAC
GAGAAAATCCCATGGAAGGAGTCCCCTTCGATCTCGAGCAATTCTCTGAATCTCTCGCTAAGAACGATGGCGTCAATTGCGAGATTGGCACTACGGTTAAAGGTATGGTATTTAAGACCGATGGAAATGGGGCAATGGTTGACATCCGTGATAAGGTTCCTGCATTTTTACCTCTAGTAGAGGCGTGTATTTATAACATT
AAGACCCCAGAAGAAGTAGGCCTTTACCCAGGCATAGAAGAGCATTTCATGGTCCTTCGAGAGGATGATACAAATAGCAGGATGATTTTAAGCCTACAAAAACTTCAGTGCGACCTTGCATGGGAACGATGCAGACAACTCCAGACTGAGGATGTTGTTGTCAAGGGAAAGGTGGTATCTGCTAACAAAGGGGGACTTAA
TGTACTTGTAGAGTGTCTCAGAGGGTTTGTCCCTTTTTCACAAATCTCAACTAAATTAAATATAGAAGTGCTAGTGAACAAGGAAATCCCTCTGAAATTCATGGAGGTGGACGAGGAACAAACGCGTCTTGTATTGAGCAATCGGAAAGCTATGGCAGACAGTCAGGCTCAATTAGGAATTGGATCTGTGGTTCTTGGAA
CTGTGCAAACTATAAAGCCTTATGGCGCCTTTATTGATCTTGGAGGTATTAATGGCCTTCTGCATGTCAGTCAAATCAGCCATGACCGTGTTGCAGATGTTGGTACTATATTACAGCCAGGTGACACACTGAAGGTAATGATTCTCAGTCATGATCGGGAAAGAGGCAGGGTGAGCCTCTCAACAAAGAAATTGGAGCCC
ACTCCTGGTGACATGCTTCGCAACCCAAAGCTTGTATATGAAAAGGCTGACGAGATGGCTCAAACGTTTAGGCAAAGAATAGCACAAGCTGAAGCCATGGCTCGTGCTGATATGTTGCGGTTCCAACCAGAGGGTGGATTGACTATTAGCTCAGATGGGGTTTTAGAATCTACAGGATTGCGTGTTGATGAGGTAGACCT
TGAAGATGTTGTTCCCGCAGGGGAAGTATAGAAATGGCAGGGCTTACAAAGTTCTTATCTTCTGCAGATGACTGTGTGGAGGGTGGAGTTTATTCCACTGTATTGCTAAATTGAAGATGCCGTTGAATGTGTGGTTTGTTACTTGACAGGTTCTGAAGTGTTCACAACAGAAATACTATTAGATGCCTACAAAGAAAATT
TCTGCTCAACTTCAGATGATGGAAAATGTTAAATTTGCTGTTTGAAAATCTCGTGTAAATGTTCTACATGTAAAGTTGTTTTAAATACAGTGTTTATTTTGATCAATTCAAATGCCCAGCTTTTATTTTTGTGGTATATGGACTGATATTCATCTTTTGTGTTCACAATTCAGAGGCATCAGAGGTAATTGTGATAGTTC
TTGAAGTATTTTCTTGTTCCTACAATTTTTCATGCTTGGTTCTTGATGTGGTTGAATGCTAGAATTTGTGCGTGCAATCCACATTGATTGGGCG

>TRINITY_DN45068_c0_g2_i4 from sample A6 / Example unspliced isoform:

GGAAGATTAATTGGCTTTCAAAAGAATGATACCCAAAGATATCCTTGGTGGTATTTTCAGCTTTCTTATCCAAAACCCACCTACGATAGTCCACTCTCCTCTCATTCTCATTTCACATTTCAGACGCTCACTGCTGCTGATTGTCTTACGATCATGGCAGCAGTAACCCAGCAATTGGTCCTCTCAGGGGTCTATTTCAC
ATGCTCCCGCAGTAGCAAACTGTGCTGCTCAAGGCCGAGGCAATGGCAGTCACAGTACCAGTCCCTGTCTCACCCCCAGGGGAAGAACCAGAAGAAGAACAAGAGCAGAGGCGTTGTGTGTGCAGTGGCGATTTCGAATGCAAAGACGCGAGAGAGGGAATCCCTCATGGAGGAAATCGAAGAGAGATTGAGGAAAATAC
GAGAAAATCCCATGGAAGGAGTCCCCTTCGATCTCGAGCAATTCTCTGAATCTCTCGCTAAGAACGATGGCGTCAATTGCGAGATTGGCACTACGGTTAAAGGTATGGTATTTAAGACCGATGGAAATGGGGCAATGGTTGACATCCGTGATAAGGTTCCTGCATTTTTACCTCTAGTAGAGGCGTGTATTTATAACATT
AAGACCCCAGAAGAAGTAGGCCTTTACCCAGGCATAGAAGAGCATTTCATGGTCCTTCGAGAGGATGATACAAATAGCAGGATGATTTTAAGCCTACAAAAACTTCAGTGCGACCTTGCATGGGAACGATGCAGACAACTCCAGACTGAGGATGTTGTTGTCAAGGGAAAGGTGGTATCTGCTAACAAAGGGGGACTTAA
TGTACTTGTAGAGTGTCTCAGAGGGTTTGTCCCTTTTTCACAAATCTCAACTAAATTAAATATAGAAGTGCTAGTGAACAAGGAAATCCCTCTGAAATTCATGGAGGTGGACGAGGAACAAACGCGTCTTGTATTGAGCAATCGGAAAGCTATGGCAGACAGTCAGGCTCAATTAGGAATTGGATCTGTGGTTCTTGGAA
CTGTGCAAACTATAAAGCCTTATGGCGCCTTTATTGATCTTGGAGGTATTAATGGCCTTCTGCATGTCAGTCAAATCAGCCATGACCGTGTTGCAGATGTTGGTACTATATTACAGCCAGGTGACACACTGAAGGTAATGATTCTCAGTCATGATCGGGAAAGAGGCAGGGTGAGCCTCTCAACAAAGAAATTGGAGCCC
ACTCCTGGTGACATGCTTCGCAACCCAAAGCTTGTATATGAAAAGGCTGACGAGATGGCTCAAACGTTTAGGCAAAGAATAGCACAAGCTGAAGCCATGGCTCGTGCTGATATGTTGCGGTTCCAACCAGAGGGTGGATTGACTATTAGCTCAGATGGGGTTTTAGAATCTACAGGATTGCGTGTTGATGAGGTAGACCT
TGAAGATGTTGTTCCCGCAGGGGAAGTATAGAAATGGCAGGGCTTACAAAGTTCTTATCTTCTGCAGATGACTgtaagtttcttatcagcctgctttgtctctgcataagaaaatgctcaagggtgtttcacaagtaacaaaggttatttctgctactgttttttcttctcatgcaagGTGTGGAGGGTGGAGTTTATTC
CACTGTATTGCTAAATTGAAGATGCCGTTGAATGTGTGGTTTGTTACTTGACAGGTTCTGAAGTGTTCACAACAGAAATACTATTAGATGCCTACAAAGAAAATTTCTGCTCAACTTCAGATGATGGAAAATGTTAAATTTGCTGTTTGAAAATCTCGTGTAAATGTTCTACATGTAAAGTTGTTTTAAATACAGTGTTT
ATTTTGATCAATTCAAATGCCCAGCTTTTATTTTTGTGGTATATGGACTGATATTCATCTTTTGTGTTCACAATTCAGAGGCATCAGAGGTAATTGTGATAGTTCTTGAAGTATTTTCTTGTTCCTACAATTTTTCATGCTTGGTTCTTGATGTGGTTGAATGCTAGAATTTGTGCGTGCAATCCACATTGATTGGGCG

Intron 2 GTAAGTTTCT ... TACTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.03	83	26.9	9.4
A2	17.14	100	27.7	21.1
A3	9.82	99	22.3	10.9
A4	20.41	32	9.4	8.6
A5	15.03	47	12.9	8.6
A6	12.53	927	212.9	135.9
A7	12.78	814	210	122.1
A8	15.1	984	217.9	180
A9	13.27	823	202	129
A10	13.82	495	147.7	81.3

>TRINITY_DN102817_c0_g1_i2 from sample A1 / Example spliced isoform

TTTCAGCTTTCTTATCCAAAACCCACCTACGATAGTCCACTCTCCTCTCATTCTCATTTCACATTTCAGACGCTCACTGCTGCTGATTGTCTTACGATCATGGCAGCAGTAACCCAGCAATTGGTCCTCTCAGGGGTCTATTTCACATGCTCCCGCAGTAGCAAACTGTGCTGCTCAAGGCCGAGGCAATGGCAGTCACA
GTACCAGTCCCTGTCTCACCCCCAGGGGAAGAACCAGAAGAAGAACAAGAGCAGAGGCGTTGTGTGTGCAGTGGCGATTTCGAATGCAAAGACGCGAGAGAGGGAATCCCTCATGGAGGAAATCGAAGAGAGATTGAGGAAAATACGAGAAAATCCCATGGAAGGAGTCCCCTTCGATCTCGAGCAATTCTCTGAATCTC
TCGCTAAGAACGATGGCGTCAATTGCGAGATTGGCACTACGGTTAAAGGTATGGTATTTAAGACCGATGGAAATGGGGCAATGGTTGACATCCGTGATAAGGTTCCTGCATTTTTACCTCTAGTAGAGGCGTGTATTTATAACATTAAGACCCCAGAAGAAGTAGGCCTTTACCCAGGCATAGAAGAGCATTTCATGGTC
CTTCGAGAGGATGATACAAATAGCAGGATGATTTTAAGCCTACAAAAACTTCAGTGCGACCTTGCATGGGAACGATGCAGACAACTCCAGACTGAGGATGTTGTTGTCAAGGGAAAGGTGGTATCTGCTAACAAAGGGGGACTTAATGTACTTGTAGAGTGTCTCAGAGGGTTTGTCCCTTTTTCACAAATCTCAACTAA
ATTAAATATAGAAGTGCTAGTGAACAAGGAAATCCCTCTGAAATTCATGGAGGTGGACGAGGAACAAACGCGTCTTGTATTGAGCAATCGGAAAGCTATGGCAGACAGTCAGGCTCAATTAGGAATTGGATCTGTGGTTCTTGGAACTGTGCAAACTATAAAGCCTTATGGCGCCTTTATTGATCTTGGAGGTATTAATG
GCCTTCTGCATGTCAGTCAAATCAGCCATGACCGTGTTGCAGATGTTGGTACTATATTACAGCCAGGTGACACACTGAAGGTAATGATTCTCAGTCATGATCGGGAAAGAGGCAGGGTGAGCCTCTCAACAAAGAAATTGGAGCCCACTCCTGGTGACATGCTTCGCAACCCAAAGCTTGTATATGAAAAGGCTGACGAG
ATGGCTCAAACGTTTAGGCAAAGAATAGCACAAGCTGAAGCCATGGCTCGTGCTGATATGTTGCGGTTCCAACCAGAGGGTGGATTGACTATTAGCTCAGATGGGGTTTTAGAATCTACAGGATTGCGTGTTGATGAGGTAGACCTTGAAGATGTTGTTCCCGCAGGGGAAGTATAGAAATGGCAGGGCTTACAAAGTTC
TTATCTTCTGCAGATGACTGTTCTGAAGTGTTCACAACAGAAATACTATTAGATGCCTACAAAGAAAATTTCTGCTCAACTTCAGATGATGGAAAATGTTAAATTTGCTGTTTGAAAATCTCGTGTAAATGTTCTACATGTAAAGTTGTTTTAAATACAGTGTTTATTTTGATCAATTCAAATGCCCAGCTTTTATTTTT
GTGGTATATGGACTGATATTCATCTTTTGTGTTCACAATTCAGAGGCATCAGAGGTAATTGTGATAGTTCTTGAAAAAAAA

>TRINITY_DN102817_c0_g1_i5 from sample A1 / Example unspliced isoform:

TTGCAATTGAGAGAGTCTTATAGTTAGTTGATATAGATATTGTATCATCAGGATATGCATGTATCATCATGATATAGAGATTGTGCCCATTTTACTAGAAATCAGTAATTAATTTGTTCTGAATAATGTGGAGGGCTAATCAATATAACTACTTGCAGGGTGGATTGACTATTAGCTCAGATGGGGTTTTAGAATCTACA
GGATTGCGTGTTGATGAGGTAGACCTTGAAGATGTTGTTCCCGCAGGGGAAGTATAGAAATGGCAGGGCTTACAAAGTTCTTATCTTCTGCAGATGACTgtaagtttcttatcagcctgctttgtctctgcataagaaaatgctcaagggtgtttcacaagtaacaaaggttatttctgctactgttttttcttctcatg
caaggtgtggagggtggagtttattccactgtattgctaaattgaagatgccgttgaatgtgtggtttgttacttgacagGTTCTGAAGTGTTCACAACAGAA

Intron 3 GTGTGGAGGG ... TACTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.02	83	28	11
A2	20.32	100	26	25
A3	13.54	99	23	8
A4	30.43	32	16	12
A5	19.66	47	13	10
A6	17.78	927	235	166
A7	18.48	814	217	152
A8	19.18	984	244	223
A9	19.51	823	237	162
A10	20.03	495	152	96

>TRINITY_DN107033_c1_g3_i1 from sample A4 / Example spliced isoform

CTTATCCAAAACCCACCTACGATAGTCCACTCTCCTCTCATTCTCATTTCACATTTCAGACGCTCACTGCTGCTGATTGTCTTACGATCATGGCAGCAGTAACCCAGCAATTGGTCCTCTCAGGGGTCTATTTCACATGCTCCCGCAGTAGCAAACTGTGCTGCTCAAGGCCGAGGCAATGGCAGTCACAGTACCAGTCC
CTGTCTCACCCCCAGGGGAAGAACCAGAAGAAGAACAAGAGCAGAGGCGTTGTGTGTGCAGTGGCGATTTCGAATGCAAAGACGCGAGAGAGGGAATCCCTCATGGAGGAAATCGAAGAGAGATTGAGGAAAATACGAGAAAATCCCATGGAAGGAGTCCCCTTCGATCTCGAGCAATTCTCTGAATCTCTCGCTAAGAA
CGATGGCGTCAATTGCGAGATTGGCACTACGGTTAAAGGTATGGTATTTAAGACCGATGGAAATGGGGCAATGGTTGACATCCGTGATAAGGTTCCTGCATTTTTACCTCTAGTAGAGGCGTGTATTTATAACATTAAGACCCCAGAAGAAGTAGGCCTTTACCCAGGCATAGAAGAGCATTTCATGGTCCTTCGAGAGG
ATGATACAAATAGCAGGATGATTTTAAGCCTACAAAAACTTCAGTGCGACCTTGCATGGGAACGATGCAGACAACTCCAGACTGAGGATGTTGTTGTCAAGGGAAAGGTGGTATCTGCTAACAAAGGGGGACTTAATGTACTTGTAGAGTGTCTCAGAGGGTTTGTCCCTTTTTCACAAATCTCAACTAAATTAAATATA
GAAGTGCTAGTGAACAAGGAAATCCCTCTGAAATTCATGGAGGTGGACGAGGAACAAACGCGTCTTGTATTGAGCAATCGGAAAGCTATGGCAGACAGTCAGGCTCAATTAGGAATTGGATCTGTGGTTCTTGGAACTGTGCAAACTATAAAGCCTTATGGCGCCTTTATTGATCTTGGAGGTATTAATGGCCTTCTGCA
TGTCAGTCAAATCAGCCATGACCGTGTTGCAGATGTTGGTACTATATTACAGCCAGGTGACACACTGAAGGTAATGATTCTCAGTCATGATCGGGAAAGAGGCAGGGTGAGCCTCTCAACAAAGAAATTGGAGCCCACTCCTGGTGACATGCTTCGCAACCCAAAGCTTGTATATGAAAAGGCTGACGAGATGGCTCAAA
CGTTTAGGCAAAGAATAGCACAAGCTGAAGCCATGGCTCGTGCTGATATGTTGCGGTTCCAACCAGAGGGTGGATTGACTATTAGCTCAGATGGGGTTTTAGAATCTACAGGATTGCGTGTTGATGAGGTAGACCTTGAAGATGTTGTTCCCGCAGGGGAAGTATAGAAATGGCAGGGCTTACAAAGTTCTTATCTTCTG
CAGATGACTGTTCTGAAGTGTTCACAACAGAAATACTATTAGATGCCTACAAAGAAAATTTCTGCTCAACTTCAGATGATGGAAAATGTTAAATTTGCTGTTTGAAAATCTCGTGTAAATGTTCTACATGTAAAGTTGTTTTAAATACAGTGTTTATTTTGATCAATTCAAATGCCCAGCTTTTATTTTTGTGGTATATG
GACTGATATTCATCTTTTGTGTTCACAATTCAGAGGCATCAGAGGTAATTGTGATAGTTCTTGAAGTATTTTCTTGTTCCTAAAAAA

>TRINITY_DN107033_c1_g3_i4 from sample A4 / Example unspliced isoform:

CTTATCCAAAACCCACCTACGATAGTCCACTCTCCTCTCATTCTCATTTCACATTTCAGACGCTCACTGCTGCTGATTGTCTTACGATCATGGCAGCAGTAACCCAGCAATTGGTCCTCTCAGGGGTCTATTTCACATGCTCCCGCAGTAGCAAACTGTGCTGCTCAAGGCCGAGGCAATGGCAGTCACAGTACCAGTCC
CTGTCTCACCCCCAGGGGAAGAACCAGAAGAAGAACAAGAGCAGAGGCGTTGTGTGTGCAGTGGCGATTTCGAATGCAAAGACGCGAGAGAGGGAATCCCTCATGGAGGAAATCGAAGAGAGATTGAGGAAAATACGAGAAAATCCCATGGAAGGAGTCCCCTTCGATCTCGAGCAATTCTCTGAATCTCTCGCTAAGAA
CGATGGCGTCAATTGCGAGATTGGCACTACGGTTAAAGGTATGGTATTTAAGACCGATGGAAATGGGGCAATGGTTGACATCCGTGATAAGGTTCCTGCATTTTTACCTCTAGTAGAGGCGTGTATTTATAACATTAAGACCCCAGAAGAAGTAGGCCTTTACCCAGGCATAGAAGAGCATTTCATGGTCCTTCGAGAGG
ATGATACAAATAGCAGGATGATTTTAAGCCTACAAAAACTTCAGTGCGACCTTGCATGGGAACGATGCAGACAACTCCAGACTGAGGATGTTGTTGTCAAGGGAAAGGTGGTATCTGCTAACAAAGGGGGACTTAATGTACTTGTAGAGTGTCTCAGAGGGTTTGTCCCTTTTTCACAAATCTCAACTAAATTAAATATA
GAAGTGCTAGTGAACAAGGAAATCCCTCTGAAATTCATGGAGGTGGACGAGGAACAAACGCGTCTTGTATTGAGCAATCGGAAAGCTATGGCAGACAGTCAGGCTCAATTAGGAATTGGATCTGTGGTTCTTGGAACTGTGCAAACTATAAAGCCTTATGGCGCCTTTATTGATCTTGGAGGTATTAATGGCCTTCTGCA
TGTCAGTCAAATCAGCCATGACCGTGTTGCAGATGTTGGTACTATATTACAGCCAGGTGACACACTGAAGGTAATGATTCTCAGTCATGATCGGGAAAGAGGCAGGGTGAGCCTCTCAACAAAGAAATTGGAGCCCACTCCTGGTGACATGCTTCGCAACCCAAAGCTTGTATATGAAAAGGCTGACGAGATGGCTCAAA
CGTTTAGGCAAAGAATAGCACAAGCTGAAGCCATGGCTCGTGCTGATATGTTGCGGTTCCAACCAGAGGGTGGATTGACTATTAGCTCAGATGGGGTTTTAGAATCTACAGGATTGCGTGTTGATGAGGTAGACCTTGAAGATGTTGTTCCCGCAGGGGAAGTATAGAAATGGCAGGGCTTACAAAGTTCTTATCTTCTG
CAGATGACTgtgtggagggtggagtttattccactgtattgctaaattgaagatgccgttgaatgtgtggtttgttacttgacagGTTCTGAAGTGTTCACAACAGAAATACTATTAGATGCCTACAAAGAAAATTTCTGCTCAACTTCAGATGATGGAAAATGTTAAATTTGCTGTTTGAAAATCTCGTGTAAATGTTC
TACATGTAAAGTTGTTTTAAATACAGTGTTTATTTTGATCAATTCAAATGCCCAGCTTTTATTTTTGTGGTATATGGACTGATATTCATCTTTTGTGTTCACAATTCAGAGGCATCAGAGGTAATTGTGATAGTTCTTGAAGTATTTTCTTGTTCCTAAAAAA