NA ----> NA

Intron 1 GTAAGTTATC ... CAACTGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.48	46	14	5.5
A2	25.7	45	14	17.2
A3	14.46	62	13	8
A4	19.27	55	14	12.5
A5	23.91	31	14	5.5
A6	15.27	253.7	50	41.7
A7	12.74	217.7	34	29.7
A8	12.03	172	25	22.2
A9	12.31	233	35	30.5
A10	9.42	218	16	29.5

>TRINITY_DN108605_c0_g1_i9 from sample A2 / Example spliced isoform

CATCTATGAAAGTATAACCGGGCTTCCAATGATCCTTTAACAACATGCTTCCTTGCCTAGCCCTGAATTCGTCTGAATGAAATAAATCTCAAAATAAGTCGCACGTCAATGCCATGGCTGACTTTGGACATGAAACGGAAGATGTCACAAGGAGGAGGAGGAAAACCATCTGTTGGCATCCTTTCACAATAATGCTGGTA
TTTTATTCCAGTGGTATCCGGGCTGCCTTGGAGGAAGGCGACCATCTATCTAAGTCATGCAAGAATACAATCCAGGGGAGGTACATGTTGACTGATGAGCGGGGTATGTATGTGGTGTCATGTCTGTTAATCCATCCTCTGGTTGCTGTCCTGAAGCTGGTGAACAGTTTCCCTGTCAGGGATGCAATATTAGTTCACAG
TGTTGTGATTCTTATGAATTTTGTGTATCATGCTGCTTAAATCCAGCCCGGACACACAAAGAAACCGTAATTAAGATGAAGGTGGCTAAACCAACTACTGCAGGCACCTATGGGAGTATTTTCAATTTTTGTGCTGGAAGGTGCCGCCATAATTCAGCGTGCGTGGGAGGAAGGCGACCATCTATCTAAGTCATGCAAGA
ATACAATCCAGGGGAGGTACATGTTGACTGATGAGCGGGG

>TRINITY_DN108605_c0_g1_i14 from sample A2 / Example unspliced isoform:

CATCTATGAAAGTATAACCGGGCTTCCAATGATCCTTTAACAACATGCTTCCTTGCCTAGCCCTGAATTCGTCTGAATGAAATAAATCTCAAAATAAGTCGCACGTCAATGCCATGGCTGACTTTGGACATGAAACGGAAGATGTCACAAGGAGGAGGAGGAAAACCATCTGTTGGCATCCTTTCACAATAATGCTGGTA
TTTTATTCCAGTGGTATCCGGGCTGCCTTgtaagttatcgtatcctctgtttagtttaatctctcttaaatcaaatcggggaaaattcccagactacaacaataaatttcaactcggacgcaatatattcaaaataccttgatacttttcaactgaaagGGAGGAAGGCGACCATCTATCTAAGTCATGCAAGAATACAA
TCCAGGGGAGGTACATGTTGACTGATGAGCGGGGTATGCACTAACCCTAGTTAGTTTTAATGCAGATCCTTGTCAACATATTGAAAATTATTGAACCCATTTTTATATGGAACATGATTCATGGTATCTTCTGGTAATTCTTTATGCATTTGCAGATATGAACATATCTATTTACAATGTTATCCAAAAAGTGGGATTGT
TCTAGTGGTTAATCTGTAATAGTTACCAGGTGCTGGTCAAAATATC

Intron 2 GTATATTTCT ... TTTGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.81	32	3.5	10
A2	19.51	33	2	8
A3	25.86	43	2.5	15
A4	23.53	26	3	8
A5	0	6	0.5	0
A6	22.07	286	16.5	81
A7	18.86	228	19.5	53
A8	25.22	252	17	85
A9	19.35	175	11	42
A10	19.12	110	6	26

>TRINITY_DN108605_c0_g1_i4 from sample A2 / Example spliced isoform

CATCTATGAAAGTATAACCGGGCTTCCAATGATCCTTTAACAACATGCTTCCTTGCCTAGCCCTGAATTCGTCTGAATGAAATAAATCTCAAAATAAGTCGCACGTCAATGCCATGGCTGACTTTGGACATGAAACGGAAGATGTCACAAGGAGGAGGAGGAAAACCATCTGTTGGCATCCTTTCACAATAATGCTGGTA
TTTTATTCCAGTGGTATCCGGGCTGCCTTGTAAGTTATCGTATCCTCTGTTTAGTTTAATCTCTCTTAAATCAAATCGGGGAAAATTCCCAGACTACAACAATAAATTTCAACTCGGACGCAATATATTCAAAATACCTTGATACTTTTCAACTGAAAGGGAGGAAGGCGACCATCTATCTAAGTCATGCAAGAATACAA
TCCAGGGGAGGTACATGTTGACTGATGAGCGGGGGTATGTATGTGGTGTCATGTCTGTTAATCCATCCTCTGGTTGCTGTCCTGAAGCTGGTGAACAGTTTCCCTGTCAGGGATGCAATATTAGTTCACAGTGTTGTGATTCTTATGAATTTTGTGTATCATGCTGCTTAAATCCAGCCCGGACACACAAAGAAACCGTA
ATTAAGATGAAGGTGGCTAAACCAACTACTGCAGGCACCTATGGGAGTATTTTCAATTTTTGTGCTGGAAGGTGCCGCCATAATTCAGCGTGCGTGGTCCATGAGAATGCTTATGCAAGTGAAATGCATCACTGCTTTTCCTCTCAGCTAAATTCATCTGGTACTGTCAAACCTCAATCTGAGGTGGAATTGTCAGACAT
TTCCATTATAATAGGCAGGCAAGGTGAATCATGTACCTTGGCTTGCAAGTCCAGGGGACAGTCTTGCATATCGAACAGGTTTTTGGCCCTAAATAACTGTGGAGTTCTACAGAAATATATGAGCTGTAAAGGGGCATGTATAGCCAGCATAGGGTCAGATCAACCAGCAGAAGTTATCGATAATGCCCCAAGACATCTGC
ATCCTGGGGCATGCATGTATAGCAAAAAGCAGGCTATTTTGTCATGTGATGGTTCCCATCCATACACCCGCAGGCTTTGTCCATGTGCATAGCTGGCAGATTAAGTACAGTTTGTTTCTAGAAAGCTTCCGGAATGATTCTCTAAATAGTTCCATGCAACTAGAAGATTATGAATAGAGGCAAATCTGGGATTAAGCTCC
TCGGTACTTTTGAGGGTTGCATCCTCAATGTATTCCAGTGTTGCGATTAAATCAATCTACTTATTTCATGATTGGCTGGATATCATAATACCATCTTCGTGACAGCTTTTCTGATGCTGCATCTGGTGAGTTGAATTTGAAGGTAAAATGTATATTCCCAAAATTGTAGTGATATGGCATTAACTTGGATTCCGGGTAG

>TRINITY_DN108605_c0_g1_i2 from sample A2 / Example unspliced isoform:

CATCTATGAAAGTATAACCGGGCTTCCAATGATCCTTTAACAACATGCTTCCTTGCCTAGCCCTGAATTCGTCTGAATGAAATAAATCTCAAAATAAGTCGCACGTCAATGCCATGGCTGACTTTGGACATGAAACGGAAGATGTCACAAGGAGGAGGAGGAAAACCATCTGTTGGCATCCTTTCACAATAATGCTGGTA
TTTTATTCCAGTGGTATCCGGGCTGCCTTGGAGGAAGGCGACCATCTATCTAAGTCATGCAAGAATACAATCCAGGGGAGGTACATGTTGACTGATGAGCGGGGGTATGTATGTGGTGTCATGTCTGTTAATCCATCCTCTGGTTGCTGTCCTGAAGCTGGTGAACAGTTTCCCTGTCAGGGATGCAATATTAGTTCACA
GTGTTGTGATTCTTATGAATTTTGTGTATCATGCTGCTTAAATCCAGCCCGGACACACAAAGAAACCGTAATTAAGATGAAGGTGGCTAAACCAACTACTGCAGGCACCTATGGGAGTATTTTCAATTTTTGTGCTGGAAGGTGCCGCCATAATTCAGCGTGCGTGGTCCATGAGAATGCTTATGCAAGTGAAATGCATC
ACTGCTTTTCCTCTCAGCTAAATTCATCTGGTACTGTCAAACCTCAATCTGAGGTGGAATTGTCAGACATTTCCATTATAATAGGCAGGCAAGGTGAATCATGTACCTTGGCTTGCAAGTCCAGGGGACAGTCTTGCATATCGAACAGGTTTTTGGCCCTAAATAACTGTGGAGTTCTACAGAAATATATGAGCTGTAAA
GGGGCATGTATAGCCAGCATAGGGTCAGATCAACCAGCAGAAGTTATCGATAATGCCCCAAGACATCTGgtatatttctaacttttgtcatataattctttatataaatgttcttattctcacgcatttcattacttagccgttactgttggatattgtggacatggattatttccctacagaatggcatcgtattgatg
tatgcagctgtttgcatgtgggcatttgtttcagCATCCTGGGGCATGCATGTATAGCAAAAAGCAGGCTATTTTGTCATGTGATGGTTCCCATCCATACACCCGCAGGCTTTGTCCATGTGCATAGCTGGCAGATTAAGTACAGTTTGTTTCTAGAAAGCTTCCGGAATGATTCTCTAAATAGTTCCATGCAACTAGAA
GATTATGAATAGAGGCAAATCTGGGATTAAGCTCCTCGGTACTTTTGAGGGTTGCATCCTCAATGTATTCCAGTGTTGCGATTAAATCAATCTACTTATTTCATGATTGGCTGGATATCATAATACCATCTTCGTGACAGCTTTTCTGATGCTGCATCTGGTGAGTTGAATTTGAAGGTAAAATGTATATTCCCAAAATT
GTAGTGATATGGCATTAACTTGGATTCCGGGTAG

Intron 3 GTATGCTACC ... TGTGGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.81	46	16	6
A2	25.71	26	9	1
A3	11.29	55	7	0
A4	11.9	37	5	0
A5	22.22	7	2	4
A6	13.77	238	38	2
A7	20.17	186	47	1
A8	17.67	177	38	0
A9	11.32	188	24	4
A10	15.28	122	22	0

>TRINITY_DN116282_c1_g1_i4 from sample A1 / Example spliced isoform

CTCTACTCTGCAATTTGGTTATAGATAACTTCTTAACTCATTTAGTAGTTTGTTTGGAATTCATTCAGCATATAAATTGACTTGTTTCTTGTACAGGGGATGCAATATTAGTTCACAGTGTTGTGATTCTTATGAATTTTGTGTATCATGCTGCTTAAATCCAGCCCGGACACACAAAGAAACCGTAATTAAGATGAAGG
TGGCTAAACCAACTACTGCAGGCACCTATGGGAGTATTTTCAATTTTTGTGCTGGAAGGTGCCGCCATAATTCAGCGTGCGTGGTCCATGAGAATGCTTATGCAAGTGAAATGCATCACTGCTTTTCCTCTCAGCTAAATTCATCTGGTACTGTCAAACCTCAATCTGAGGTGGAATTGTCAGACATTTCCATTATAATA
GGCAGGCAAGGTGAATCATGTACCTTGGCTTGCAAGTCCAGGGGACAGTCTTGCATATCGAACAGGTTTTTGGCCCTAAATAACTGTGGAGTTCTACAGAAATATATGAGCTGTAAAGGGGCATGTATAGCCAGCATAGGGTCAGATCAACCAGCAGAAGTTATCGATAATGCCCCAAGACATCTGCATCCTGGGGCATG
CATGTATAGCAAAAAGCAGGCTATTTTGTCATGTGATGGTTCCCATCCATACACCCGCAGGCTTTGTCCATGTGCATAGCTGGCAGATTAAGTACAGTTTGTTTCTAGAAAGCTTCCGGAATGATTCTCTAAATAGTTCCATGCAACTAGAAGATTATGAATAGAGGCAAATCTGGGATTAAGCTCCTCGGTACTTTTGA
GGGTTGCATCCTCAATGTATTCCAGTGTTGCGATTAAATCAATCTACTTATTTCATGATTGGCTGGATATCATAATACCATCTTCGTGACAGCTTTTCTGATGCTGCATCTGGTGAGTTGAATTTGAAGGTAAAATGTATATTCCCAAAATTGTAGTGATATGGCATTAACTTGGATTCCGGGTAGATGTTTGAATTTTG
AGTTGTATTTAACACTTTCAGCATAACATGATCTTTCAATATTACAAGATAGTTACTGGAA

>TRINITY_DN116282_c1_g1_i1 from sample A1 / Example unspliced isoform:

CCGCCAATTCTCATCTATGAAAGTATAACCGGGCTTCCAATGATCCTTTAACAACATGCTTCCTTGCCTAGCCCTGAATTCGTCTGAATGAAATAAATCTCAAAATAAGTCGCACGTCAATGCCATGGCTGACTTTGGACATGAAACGGAAGATGTCACAAGGAGGAGGAGGAAAACCATCTGTTGGCATCCTTTCACAA
TAATGCTGGTATTTTATTCCAGTGGTATCCGGGCTGCCTTGGAGGAAGGCGACCATCTATCTAAGTCATGCAAGAATACAATCCAGGGGAGGTACATGTTGACTGATGAGCGGGGGTATGTATGTGGTGTCATGTCTGTTAATCCATCCTCTGGTTGCTGTCCTGAAGCTGGTGAACAGTTTCCCTGTCAGGGATGCAAT
ATTAGTTCACAGTGTTGTGATTCTTATGAATTTTGTGTATCATGCTGCTTAAATCCAGCCCGGACACACAAAGAAACCGTAATTAAGATGAAGGTGGCTAAACCAACTACTGCAGGCACCTATGGGAGTATTTTCAATTTTTGTGCTGGAAGGTGCCGCCATAATTCAGCGTGCGTGGTCCATGAGAATGCTTATGCAAG
TGAAATGCATCACTGCTTTTCCTCTCAGCTAAATTCATCTGGTACTGTCAAACCTCAATCTGAGGTGGAATTGTCAGACATTTCCATTATAATAGGCAGgtatgctaccctcgcataatttaggttttggatctgagcagttgttgagatttccatgtggttcctaatgctaaagtttacaaatgtgtggaacagGCAAG
GTGAATCATGTACCTTGGCTTGCAAGTCCAGGGGACAGTCTTGCATATCGAACAGGTTTTTGGCCCTAAATAACTGTGGAGTGTACGTTTCAGCTCCATTTGTTATTTGTCACAACAATCTCATCTTGTTTTCTTCCG