NA ----> NA

Intron 1 GTGCGTACTT ... AGGATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.4	93	4	244
A2	69.19	114	9	256
A3	71.43	104	9.9	260
A4	72.22	85	9	221
A5	69.24	72.9	13	164
A6	65.44	319.6	34	605
A7	67.49	484	31.8	1005
A8	69.14	304	25	681
A9	70.31	434.9	17.8	1030
A10	70.43	342.9	13	817

>TRINITY_DN103417_c0_g1_i1 from sample A1 / Example spliced isoform

TGTTGCGCTCGTTACTTGCAGAGGACGGAACTTCAGTGGGAAGTATTGTGAAAACGCAACAAGGAATACCTACCACTGCCTATATAAACTCCTCGAGGCAGATATAGCTCATCAGGAGGCAGATATAGCTCATCAGGTGGAGGAGAGTGGGGAGATGGCTAGCCCAGGAACTGGATGGGCCGGTTGGGCTCGTCGCAAGC
TAGATCCTCTTATCGTAATTCTCAAAAGGGGTGCAGAGCCAAAGCAATTAGCGTTGTCTTCAGCTGTGGGCGTTACCTTGGGTATTTTCCCTATTTGCGGTACAACAGTGATACTATGTGGGGTGGCTGCTGCTTTGCTTGGTGCCAGTATTCACACACCCACCCTGATGCTTGCCAACTTTGTTGCCATGCCCATAGAG
TTGAG

>TRINITY_DN103417_c0_g1_i3 from sample A1 / Example unspliced isoform:

TGTTGCGCTCGTTACTTGCAGAGGACGGAACTTCAGTGGGAAGTATTGTGAAAACGCAACAAGGAATACCTACCACTGCCTATATAAACTCCTCGAGgtgcgtactttctaatacgcccttcattgtgctgaacagaacaacatattgttagggacgtgtcctaaggagtcatagtaagttcaaggattgcagGCAGATA
TAGCTCATCAGGAGGCAGATATAGCTCATCAGGTGGAGGAGAGTGGGGAGATGGCTAGCCCAGGAACTGGATGGGCCGGTTGGGCTCGTCGCAAGCTAGATCCTCTTATCGTAATTCTCAAAAGGGGTGCAGAGCCAAAGCAATTAGCGTTGTCTTCAGCTGTGGGCGTTACCTTGGGTATTTTCCCTATTTGCGGTACA
ACAGTGATACTATGTGGGGTGGCTGCTGCTTTGCTTGGTGCCAGTATTCACACACCCACCCTGATGCTTGCCAACTTTGTTGCCATGCCCATAGAGTTGAGGACATTTGGAGAGCAACAGTTTCCTTAATGCCATCAGTCTTTATCCTGCTTTGTAGTTTGGTGGTGCCTTTCTTGCGTCTTGGTGAAGTCATTTCTGGT
GGGCCTCATTTTGCTTTGACTTCAGACGCACTGAAGATGGTCATAACTGGTAAAGCTTCTCGTGATGTTCTCCTTGGAGTCTCTCACGCGATGCTTGGCTGGCTGGTTGCAGCTCCCTTTATCTTGGTTGTACTGTATGTTGTTTTTATTCCCTGCTTCAAATTCTTAGTTCAGAAATTCAGTTCTCATCCAACAAGTCC
AAAGAAGGCACATCCTCACCCAGAACCCAAGCCCAAGATCAGGGATGCCTGATTTCTTTTAGATAAATAAGAACAGAGAGAAGTTCAAAAGGGATGGACATTTAACAGCTCATGTGAATTGTTCTAGGTTGAGGTGGTTTGAGCGACTTGTACATAAGTATGCAACATTGACAAATTTCAATCTCTAAGGACTTTCACAT
TTCTTGGTGCCTCAAGAAATCTTTCTGGCTAATCACATGCAATTATATCGGGTTATATATTTTACAAGATGTTACATTATGTATTTCACGGTTGAACATGAAGAATTCCAAAGAGTTGTACTGCACTTGATTTTATCAAGTTGGTTGCCGGTTTGTAGAGTTGGTGAGAATTCTGAAAAAGTCTATTTCTTTACAAACTA
TCTGGTCTGATAAATTTTAATCAATGGCAAGCGGCATGAACCTTATGGGTGAAGTAGGTCGCACCTGCTAGAAAAGTTATAATGTGTACACTACAAATAGAGGCTAGGTTTGATGAAATAAAATAACTCTCTGATGATATATAGCAAATTCTTATGGGTGAAGTAGGTCGCAGCTGCTAGAAAAGTTATAATGTGTACAC
TACAAATAGAGGCTAGGTCAAAGGTTATAGTAACTTTTATCTACTGATTAAATTTGAATGCTTCTTTATGTGCTTTGAGTCAAATCACATGTATCTATTTTTGAGAAAAATAATGGATGTATGCAAATCCCTTCTCAAGTAAAGAACAAGATTTACAGAGACC

Intron 2 GTGCGTACTT ... GGAGTCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.82	83	4	192
A2	73.44	64	9	177
A3	71.58	79	10	199
A4	70.09	70	9	164
A5	67.39	60	13	124
A6	63.22	260	34	447
A7	66.23	388	32	761
A8	66.03	268	25	521
A9	68.85	356	18	787
A10	68.43	292	13	633

>TRINITY_DN100499_c0_g1_i1 from sample A5 / Example spliced isoform

ATTATACTGCCATGTTGCGCTCGTTACTTGCAGAGGACGGAACTTCAGTGGGAAGTATTGTGAAAACGCAACAAGGAATACCTACCACTGCCTATATAAACTCCTCGAGTAAGTTCAAGGATTGCAGGCAGATATAGCTCATCAGGAGGCAGATATAGCTCATCAGGTGGAGGAGAGTGGGGAGATGGCTAGCCCAGGAA
CTGGATGGGCCGGTTGGGCTCGTCGCAAGCTAGATCCTCTTATCGTAATTCTCAAAAGGGGTGCAGAGCCAAAGCAATTAGCGTTGTCTTCAGCTGTGGGCGTTACCTTGGGTATTTTCCCTATTTGCGGTACAACAGTGATACTATGTGGGGTGGCTGCTGCTTTGCTTGGTGCCAGTATTCACACACCCACCCTGATG
CTTGCCAACTTTGTTGCCATGCCCATAGAGTTGAGTTTGGTGGTGCCTTTCTTGCGTCTTGGTGAAGTCATTTCTGGTGGGCCTCATTTTGCTTTGACTTCAGACGCACTGAAGATGGTCATAACTGGTAAAGCTTCTCGTGATGTTCTCCTTGGAGTCTCTCACGCGATGCTTGGCTGGCTGGTTGCAGCTCCCTTTAT
CTTGGTTGTACTGTATGTTGTTTTTATTCCCTGCTTCAAATTCTTAGTTCAGAAATTCAGTTCTCATCCAACAAGTCCAAAGAAGGCACATCCTCACCCAGAACCCAAGCCCAAGATCAGGGATGCCTGATTTCTTTTAGATAAATAAGAACAGAGAGAAGTTCAAAAGGGATGGACATTTAACAGCTCATGTGAATTGT
TCTAGGTTGAGGTGGTTTGAGCGACTTGTACATAAGTATGCAACATTGACAAATTTCAATCTCTAAGGACTTTCACATTTCTTGGTGCCTCAAGAAATCTTTCTGGCTAATCACATGCAATTATATCGGGTTATATATTTTACAAGATGTTACATTATGTATTTCACGGTTGAACATGAAGAATTCCAAAGAGTTGTACT
GCACTTGATTTTATCAAGTTGGTTGCCGGTTTGTAGAGTTGGTGAGAATTCTGAAAAAGTCTATTTCTTTACAAACTATCTGGTCTGATAAATTTTAATCAATGGCAAGCGGCATGAACCTTATGGGTGAAGTAGGTCGCACCTGCTAGAAAAGTTATAATGTGTACACTACAAATAGAGGCTAGGTTTGATGAAATAAA
ATAACTCTCTGATGATATATAGCAAATTCTTATGGGTGAAGTAGGTCGCAGCTGCTAGAAAAGTTATAATGTGTACACTACAAATAGAGGCTAGGT

>TRINITY_DN100499_c0_g1_i3 from sample A5 / Example unspliced isoform:

ATTATACTGCCATGTTGCGCTCGTTACTTGCAGAGGACGGAACTTCAGTGGGAAGTATTGTGAAAACGCAACAAGGAATACCTACCACTGCCTATATAAACTCCTCGAGgtgcgtactttctaatacgcccttcattgtgctgaacagaacaacatattgttagggacgtgtcctaaggagtcatagTAAGTTCAAGGAT
TGCAGGCAGATATAGCTCATCAGGAGGCAGATATAGCTCATCAGGTGGAGGAGAGTGGGGAGATGGCTAGCCCAGGAACTGGATGGGCCGGTTGGGCTCGTCGCAAGCTAGATCCTCTTATCGTAATTCTCAAAAGGGGTGCAGAGCCAAAGCAATTAGCGTTGTCTTCAGCTGTGGGCGTTACCTTGGGTATTTTCCCT
ATTTGCGGTACAACAGTGATACTATGTGGGGTGGCTGCTGCTTTGCTTGGTGCCAGTATTCACACACCCACCCTGATGCTTGCCAACTTTGTTGCCATGCCCATAGAGTTGAGTTTGGTGGTGCCTTTCTTGCGTCTTGGTGAAGTCATTTCTGGTGGGCCTCATTTTGCTTTGACTTCAGACGCACTGAAGATGGTCAT
AACTGGTAAAGCTTCTCGTGATGTTCTCCTTGGAGTCTCTCACGCGATGCTTGGCTGGCTGGTTGCAGCTCCCTTTATCTTGGTTGTACTGTATGTTGTTTTTATTCCCTGCTTCAAATTCTTAGTTCAGAAATTCAGTTCTCATCCAACAAGTCCAAAGAAGGCACATCCTCACCCAGAACCCAAGCCCAAGATCAGGG
ATGCCTGATTTCTTTTAGATAAATAAGAACAGAGAGAAGTTCAAAAGGGATGGACATTTAACAGCTCATGTGAATTGTTCTAGGTTGAGGTGGTTTGAGCGACTTGTACATAAGTATGCAACATTGACAAATTTCAATCTCTAAGGACTTTCACATTTCTTGGTGCCTCAAGAAATCTTTCTGGCTAATCACATGCAATT
ATATCGGGTTATATATTTTACAAGATGTTACATTATGTATTTCACGGTTGAACATGAAGAATTCCAAAGAGTTGTACTGCACTTGATTTTATCAAGTTGGTTGCCGGTTTGTAGAGTTGGTGAGAATTCTGAAAAAGTCTATTTCTTTACAAACTATCTGGTCTGATAAATTTTAATCAATGGCAAGCGGCATGAACCTT
ATGGGTGAAGTAGGTCGCACCTGCTAGAAAAGTTATAATGTGTACACTACAAATAGAGGCTAGGTTTGATGAAATAAAATAACTCTCTGATGATATATAGCAAATTCTTATGGGTGAAGTAGGTCGCAGCTGCTAGAAAAGTTATAATGTGTACACTACAAATAGAGGCTAGGT

Intron 3 GTAAATCTAC ... TTATTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.91	348	0	61
A2	19.33	338	1	81
A3	22.96	302	0	90
A4	19.48	186	0	45
A5	18.62	153	0	35
A6	18.88	1173	0	273
A7	18.23	1570	0	350
A8	18.28	1520	1	340
A9	19.32	1470	0	352
A10	19.19	1238	1	294

>TRINITY_DN105244_c0_g1_i5 from sample A2 / Example spliced isoform

GTAGGGTTCTGATTTGTGGGTATTAGTCTGAAATTTGCTTAATATAACCAGCTAACGGGGGTGCAGAGCCAAAGCAATTAGCGTTGTCTTCAGCTGTGGGCGTTACCTTGGGTATTTTCCCTATTTGCGGTACAACAGTGATACTATGTGGGGTGGCTGCTGCTTTGCTTGGTGCCAGTATTCACACACCCACCCTGATG
CTTGCCAACTTTGTTGCCATGCCCATAGAGTTGAGTTTGGTGGTGCCTTTCTTGCGTCTTGGTGAAGTCATTTCTGGTGGGCCTCATTTTGCTTTGACTTCAGACGCACTGAAGATGGTCATAACTGGTAAAGCTTCTCGTGATGTTCTCCTTGGAGTCTCTCACGCGATGCTTGGCTGGCTGGTTGCAGCTCCCTTTAT
CTTGGTTGTACTGTATGTTGTTTTTATTCCCTGCTTCAAATTCTTAGTTCAGAAATTCAGTTCTCATCCAACAAGTCCAAAGAAGGCACATCCTCACCCAGAACCCAAGCCCAAGATCAGGGATGCCTGATTTCTTTTAGATAAATAAGAACAGAGAGAAGTTCAAAAGGGATGGACATTTAACAGCTCATGTGAATTGT
TCTAGGTTGAGGTGGTTTGAGCGACTTGTACATAAGTATGCAACATTGACAAATTTCAATCTCTAAGGACTTTCACATTTCTTGGTGCCTCAAGAAATCTTTCTGGCTAATCACATGCAATTATATCGGGTTATATATTTTACAAGATGTTACATTATGTATTTCACGGTTGAACATGAAGAATTCCAAAGAGTTGTACT
GCACTTGATTTTATCAAGTTGGTTGCCGGTTTGTAGAGTTGGTGAGAATTCTGAAAAAGTCTATTTCTTTACAAACTATCTGGTCTGATAAATTTTAATCAATGGCAAGCGGCATGAACCTTATGGGTGAAGTAGGTCGCACCTGCTAGAAAAGTTATAATGTGTACACTACAAATAGAGGCTAGGTTTGATGAAATAAA
ATAACTCTCTGATGATATATAGCAAATTCTTATGGGTGAAGTAGGTCGCAGCTGCTAGAAAAGTTATAATGTGTACACT

>TRINITY_DN105244_c0_g1_i4 from sample A2 / Example unspliced isoform:

TTCTCCTTGGAGTCTCTCACGCGgtaaatctacttgtcagacttgcatgttacagaagcttcactttcagtattggagtttatctatgaagtgctgacaatttttctttccttattggcagATGCTTGGCTGGCTGGTTGCAGCTCCCTTTATCTTGGTTGTACTGTATGTTGTTTTTATTCCCTGCTTCAAATTCTTAG
TTCAGAAATTCAGTTCTCATCCAACAAGTCCAAAGAAGGCACATCCTCACCCAGAACCCAAGCCCAAGATCAGGGATGCCTGATTTCTTTTAGATAAATAAGAACAGAGAGAAGTTCAAAAGGGATGGACATTTAACAGCTCATGTGAATTGTTCTAGGTTGAGGTGGTTTGAGCGACTTGTACATAAGTATGCAACATT
GACAAATTTCAATCTCTAAGGACTTTCACATTTCTTGGTGCCTCAAGAAATCTTTCTGGCTAATCACATGCAATTATATCGGGTTATATATTTTACAAGATGTTACATTATGTATTTCACGGTTGAACATGAAGAATTCCAAAGAGTTGTACTGCACTTGATTTTATCAAGTTGGTTGCCGGTTTGTAGAGTTGGTGAGA
ATTCTGAAAAAGTCTATTTCTTTACAAACTATCTGGTCTGATAAATTTTAATCAATGGCAAGCGGCATGAACCTTATGGGTGAAGTAGGTCGCACCTGCTAGAAAAGTTATAATGTGTACACTACAAATAGAGGCTAGGTTTGATGAAATAAAATAACTCTCTGATGATATATAGCAAATTCTTATGGGTGAAGTAGGTC
GCAGCTGCTAGAAAAGTTATAATGTGTACACT