sp|Q8GW48|HSBP_ARATH ----> Heat shock factor-binding protein

Intron 1 GTAAGATTTG ... TGAATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.38	5	6	5
A2	-1	0	0	0
A3	50	1	1	1
A4	55.55	0.7	1	0
A5	33.33	4	0	4
A6	31.43	19.7	4	14
A7	34.27	20.3	8	13
A8	49.7	11.7	13	9
A9	37.14	22	11	15
A10	52.95	14.7	21	12

>TRINITY_DN46084_c1_g4_i9 from sample A7 / Example spliced isoform

TTTTAACAGTTGCTCCCTCTGCAGCCATTTTTCCTCCCTCTTCTTCCTCTGTCGCTGTTTGCGCCTTTCACTTGCCATGGACATCCTCTAATTTAACTACGACTCTCTCTTGACTTTGCTTGGAGCATCTTTCCATGTCATCAACCTGGAACTCTGAAAAATCTGCAGCAAATATATTTCGTTCTTTTCCCTTGGATCTT
TCTATTTCAGGATTCATTTAATTCCATGTATGGATGTCTTGGAATAGACAAGCCTGTAATTGCTTACATTAATTTGTTTCGAAGGATGATTCAGCCTAAAGAGCAAAAATGGTCTTGAAGTTTGGACGCTATTCCTATATTGCTTCATTTTGAGCACTGCAGAATGCAATTGCACCTAGTATCTGTAGAGGTTTTTGACA
AAAACAAATTTATCAATTCAACGGAGCTCTTAAAAGAAGCTCCTGAAGGTTTCTATTATGCTCTTCTTCCTTGTTGTTTCCTCCAGGATTATTCTAATCTAAGCTTGGATTGGCTTGTTTGGATTGATTGATCATTGATGATGTGTATTTCTCATCCAAAATCAATGAGTGACTTGTGCAATTATGGCCCAAGAAACAAA
GAATTATCAAGCATCAAGTGACATGATGAGAATTATAAAATCCTCCTGGGATGATACATAGTATGCTAAAGATGGAGAAAATATTCTTGGAAGGATTACATTCCACCAATCAGAGCCTAGGTCATCTTGAATTAAGCAATTCCTGTTACTGGGAATAAAGGTACATAGTAAGCTAAAGATGGAGATTCTCCTTGGAAGGA
TTCCTTTCCACCAATCAGTGCCTAGATCATCTTGAATTAAGCAATTCCTGTAACTGGGAATAATTTGTTTTCATTATTTCATGCTTTGGTTATTTGATTCCAACCGTTGATGTCTTTTTAAGTTTACCCTTATGGTTATTCTAATCTAAATGGTAGCTCATATTTAGGGCTTGTGGC

>TRINITY_DN46084_c1_g4_i1 from sample A7 / Example unspliced isoform:

ATTCAATTACTGCAGATAAGGTAGAACTCTAAACGATATCATCTGCAAGCCTGGATCAATTGCAGGATCTTTCTATTTCAGGATTCATTTAATTCCATGTATGGATGTCTTGGAATAGACAAGCCTGTAATTGCTTACATTAATTTGTTTCGAAGGATGATTCAGCCTAAAGAGCAAAAATGGTCTTGAAGTTTGGACGC
TATTCCTATgtaagatttgaccacctcgatagcaatctacagtttttcttaatagtatcaagtaattgaaggcagtgctagaaatgactgtcttatgttattaactttttacatttttctcctaaatgtttagttctagttctgatagatttgtcgtcttgattgatttaatttgatggcagaatttgaatttcagATTG
CTTCATTTTGAGCACTGCAGAATGCAATTGCACCTAGTATCTGTAGAGGTTTTTGACAAAAACAAATTTATCAATTCAACGGAGCTCTTAAAAGAAGCTCCTGAAGGTTTCTATTATGCTCTTCTTCCTTGTTGTTTCCTCCAGGATTATTCTAATCTAAGCTTGGATTGGCTTGTTTGGATTGATTGATCATTGATGAT
GTGTATTTCTCATCCAAAATCAATGAGTGACTTGTGCAATTATGGCCCAAGAAACAAAGAATTATCAAGCATCAAGTGACATGATGAGAATTATAAAATCCTCCTGGGATGATACATAGTATGCTAAAGATGGAGAAAATATTCTTGGAAGGATTACATTCCACCAATCAGAGCCTAGGTCATCTTGAATTAAGCAATTC
CTGTTACTGGGAATAAAGGTACATAGTAAGCTAAAGATGGAGATTCTCCTTGGAAGGATTCCTTTCCACCAATCAGTGCCTAGATCATCTTGAATTAAGCAATTCCTGTAACTGGGAATAATTTGTTTTCATTATTTCATGCTTTGGTTATTTGATTCCAACCGTTGATGTCTTTTTAAGTTTACCCTTATGGTTATTCT
AATCTAAATGGTAGCTCATATTTAGGGCTTGTGGC

Intron 2 ATATCCTTTG ... TGATTTTCAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.31	442.6	117.1	128.8
A2	23.98	490.6	130.8	139.3
A3	25.44	525.3	153.6	161.1
A4	23.57	394.2	103.1	110.8
A5	18.32	220.7	60	43.6
A6	24.67	3570.7	966.5	1055.5
A7	24.09	3945.9	1028.8	1134.2
A8	25.4	2867.4	697.4	894.4
A9	23.9	3840.1	978.5	1095.7
A10	23.28	3678.4	951.2	1012.8

>TRINITY_DN44265_c1_g1_i5 from sample A6 / Example spliced isoform

TCAAAATTGAATTCAAAATTCTTTGTAGAGGGAAAGCATAAAGCATTTCGGACATGGCAGGGGATGAGTCACGACCCTTTTTTTTTTAAGCTGCCCAAATTACATTATATTGAGTTGTCCATACAAATCCGACCCACCTTCAATCGAGAGAAGGCTGCTTATAAATCTTCACCCCATTGCATTATTTTCTCAAAAATTGA
CAGAAAACTCATTGTTGTACTGCGTCCGATTCAGGCCCTTGATCATGGAAAGCAGACCGGGGACTAACCAAAGTCAGGAGCAGAGGGAGACAACAAAGAACCCTGAGGATCTAACTGCATTTGTTAAAAATCTTCTTGAACAAATGCAAACCAGGTTCCAGACTATGTCACAATCTATCATCACAAGAATCGACGAGATG
GGATCTCGTATTGAGGATCTCGAAAAGAGTATTGAAGATCTTTCCATCGAAACAGCAGGGGAAGTTTCACCATCTAGTTCTTTGTCTCCTTCAGATCGGGGTGGCACAAACTGAAACAGTACTTGGAAGCAGAGGCATTCGAAACTTCGAAAGACAATGATTTCAAGTTCTATAATATATAGTAATTTTGTTTGGAAAGA
CTGGAGAAAGAGCTCTTTCTTGAAGTCATGGCTGGTTATATTGAACTTATATTTGGATATAGGTAATACTGATTTTTTTTAGTAGGTCAACATAACATGTATTGATGGAAACATACGGCAAGGTTTTGTCCAAGGAGTCGAGGTGTTGCATATTATGTTTGATTCAATTAGTACATGGTTTACAAAGGGGACTCAGCCAT
GTTCTCCTTTTGGATATATTAAGCAGGTCCTTTCAGTGATGCTGGTACTGTATTTACTTTTTCTTTTTTCCTTTCTGACGGGCTACATATTGTTTACTAGTGAAGCCGTAAGAAGGCATTGTATGGCATATCTCATAAATTGTTAGCTCCTGAACTTTGAATATGGATTGCATTTGACTTACAGGGTTTGATTACATATG
TGCCTGTCTTGGTGTTATTTATGTTTAGAAGCATGTCACTCTTGCAAGAGGAGGGATTGATTAGCCCAATTCGGGTGAATTTGGTAACTATGTTTTTGGTTATTCTTTCTGTATTGGTTTCCATTTTCCTATCTTGATTTTTATAAAAATCTATAAATTTTTACTTCAA

>TRINITY_DN44265_c1_g1_i6 from sample A6 / Example unspliced isoform:

GGAGTAATGTCTTTATCAAATTTTACATTTCCTCTACTTTCCAATTACAATATTTCTTTCTTCTTGTATATGTATAACAGGAAAGCAGACCGGGGACTAACCAAAGTCAGGAGCAGAGGGAGACAACAAAGAACCCTGAGGATCTAACTGCATTTGTTAAAAATCTTCTTGAACAAATGCAAACCAGGTTCCAGACTATG
TCACAATCTATCATCACAAGAAatatcctttgtttcatcttcactacttgtctatatacatttctattggatgaattatcttcactcatgatctgcttatgcgtttaaagatttccttttgttgtttcgcctgcgtaacatttgttgaaaaaaataagctttgcaaatgcattattcttgtacctttcaacatctgattt
gttcttccgtagttgtcttttcttgactgattttcacTCGACGAGATGGGATCTCGTA

Intron 3 GTAATCTCTT ... TGTATAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	5.48	392.9	3	34.9
A2	5.79	536.4	1.6	48.7
A3	5.52	530.9	4	47.7
A4	7.41	359.6	7.5	37.2
A5	9.27	245.8	4	30.1
A6	4.42	3200.9	0	279
A7	4.54	3787.9	0	336.3
A8	4.26	2627.2	0	210.6
A9	4.29	3655.8	1	297.1
A10	4.76	3403.3	1	310.9

>TRINITY_DN113319_c9_g2_i1 from sample A2 / Example spliced isoform

GCTGCTTATAAATCTTCACCCCATTGCATTATTTTCTCAAAAATTGACAGAAAACTCATTGTTGTACTGCGTCCGATTCAGGCCCTTGATCATGGAAAGCAGACCGGGGACTAACCAAAGTCAGGAGCAGAGGGAGACAACAAAGAACCCTGAGGATCTAACTGCATTTGTTAAAAATCTTCTTGAACAAATGCAAACCA
GGTTCCAGACTATGTCACAATCTATCATCACAAGAATCGACGAGATGGGATCTCGTATTGAGGATCTCGAAAAGAGTATTGAAGATCTTTCCATCGAAACAGCAGGGGAAGTTTCACCATCTAGTTCTTTGTCTCCTTCAGATCGGGGTGGCACAAACTGAAACAGTACTTGGAAGCAGAGGCATTCGAAACTTCGAAAG
ACAATGATTTCAAGTTCTATAATATATAGTAATTTTGTTTGGAAAGACTGGAGAAAGAGCTCTTTCTTGAAGTCATGGCTGGTTATATTGAACTTATATTTGGATATAGGTAATACTGATTTTTTTTAGTAGGTCAACATAACATGTATTGATGGAAACATACGGCAAGGTTTTGTCCAAGGAGTCGAGGTGTTGCATAT
TATGTTTGATTCAATTAGTACATGGTTTACAAAGGGGACTCAGCCATGTTCTCCTTTTGGATATATTAAGCAGGTCCTTTCAGTGATGCTGGTACTGTATTTACTTTTTCTTTTTTCCTTTCTGACGGGCTACATATTGTTTACTAGTGAAGCCGTAAGAAGGCATTGTATGGCATATCTCATAAATTGTTAGCTCCTGA
ACTTTGAATATGGATTGCATTTGACTTACAGGGTTTGATTACATATGTGCCTGTCTTGGTGTTATTTATGTTTAGAAGCATGTCACTCTTGCAAGAGGAGGGATTGATTAGCCCAATTCGGGTGAATTTGGTAACTATGTTTTTGGTTATTCTTTCTGTATTGGTTTCCATTTTCCTATCTTGATTTTTATAAAAATCTA
TAAATTTTTACTTCTATCTAATTGAATTTTCAGAAATCTTTATTTGTATTTGTGCAAAACCACTCAGGCCAAATCCAGTAAAGTGCTATGCAACTTTATTTTACAATTTCTTTCTAAAAGAGTATGATATTTCACCATGCTTTCTTTTTTTCATAGACAGGTGTGTATATGGCATTTTACGATGTTCTTTTTTTCATGGA
AATAGGTGC

>TRINITY_DN113319_c9_g2_i2 from sample A2 / Example unspliced isoform:

GCTGCTTATAAATCTTCACCCCATTGCATTATTTTCTCAAAAATTGACAGAAAACTCATTGTTGTACTGCGTCCGATTCAGGCCCTTGATCATGgtaatctctttccctacattgttatgccctggagtaatgtctttatcaaattttacatttcctctactttccaattacaatatttctttcttcttgtatatgtata
acagGAAAGCAGACCGGGGACTAACCAAAGTCAGGAGCAGAGGGAGACAACAAAGAACCCTGAGGATCTAACTGCATTTGTAAGTTTTCTCGTTTGATTTTAGATAAGTTGGGCTTAACCCACGCACCCACGCACATTAGAACAACAGATAGATGATCTCCCATTATCTGTAAAACCAATAATGGAAATGCAGAATTTGG
TATGTCTTTCTGACTTGGATGTTAATTTTTCAATGGGTAATATTTGTTCTTTGTTGTTCAAGTACGCCCAACTGCACTGCTGACCATGTAGGAATGATATACTGCATTGATTTGAGCACACTGACATTATTTCCAGAGTCGGTTCTTATTTATTTATTTTTCCTAGTGTTCAGCATTTCCAACTGCACCGCC

Intron 4 GTAAGATTTG ... TTGATGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.38	5	6	5
A2	-1	0	0	0
A3	100	0	1	0
A4	66.66	0.5	1	0
A5	-1	0	0	0
A6	31.63	10	4	5
A7	37.72	13.5	8	8
A8	56.06	8.5	13	7
A9	39.53	13	11	6
A10	61.39	8.5	21	6

>TRINITY_DN46084_c1_g4_i5 from sample A7 / Example spliced isoform

TTTTAACAGTTGCTCCCTCTGCAGCCATTTTTCCTCCCTCTTCTTCCTCTGTCGCTGTTTGCGCCTTTCACTTGCCATGGACATCCTCTAATTTAACTACGACTCTCTCTTGACTTTGCTTGGAGCATCTTTCCATGTCATCAACCTGGAACTCTGAAAAATCTGCAGCAAATATATTTCGTTCTTTTCCCTTGGATGAT
TCAGCCTAAAGAGCAAAAATGGTCTTGAAGTTTGGACGCTATTCCTATAATTTGAATTTCAGATTGCTTCATTTTGAGCACTGCAGAATGCAATTGCACCTAGTATCTGTAGAGGTTTTTGACAAAAACAAATTTATCAATTCAACGGAGCTCTTAAAAGAAGCTCCTGAAGGTTTCTATTATGCTCTTCTTCCTTGTTG
TTTCCTCCAGGATTATTCTAATCTAAGCTTGGATTGGCTTGTTTGGATTGATTGATCATTGATGATGTGTATTTCTCATCCAAAATCAATGAGTGACTTGTGCAATTATGGCCCAAGAAACAAAGAATTATCAAGCATCAAGTGACATGATGAGAATTATAAAATCCTCCTGGGATGATACATAGTATGCTAAAGATGGA
GAAAATATTCTTGGAAGGATTACATTCCACCAATCAGAGCCTAGGTCATCTTGAATTAAGCAATTCCTGTTACTGGGAATAAAGGTACATAGTAAGCTAAAGATGGAGATTCTCCTTGGAAGGATTCCTTTCCACCAATCAGTGCCTAGATCATCTTGAATTAAGCAATTCCTGTAACTGGGAATAATTTGTTTTCATTA
TTTCATGCTTTGGTTATTTGATTCCAACCGTTGATGTCTTTTTAAGTTTACCCTTATGGTTATTCTAATCTAAATGGTAGCTCATATTTAGGGCTTGTGGC

>TRINITY_DN46084_c1_g4_i1 from sample A7 / Example unspliced isoform:

ATTCAATTACTGCAGATAAGGTAGAACTCTAAACGATATCATCTGCAAGCCTGGATCAATTGCAGGATCTTTCTATTTCAGGATTCATTTAATTCCATGTATGGATGTCTTGGAATAGACAAGCCTGTAATTGCTTACATTAATTTGTTTCGAAGGATGATTCAGCCTAAAGAGCAAAAATGGTCTTGAAGTTTGGACGC
TATTCCTATgtaagatttgaccacctcgatagcaatctacagtttttcttaatagtatcaagtaattgaaggcagtgctagaaatgactgtcttatgttattaactttttacatttttctcctaaatgtttagttctagttctgatagatttgtcgtcttgattgatttaatttgatggcagAATTTGAATTTCAGATTG
CTTCATTTTGAGCACTGCAGAATGCAATTGCACCTAGTATCTGTAGAGGTTTTTGACAAAAACAAATTTATCAATTCAACGGAGCTCTTAAAAGAAGCTCCTGAAGGTTTCTATTATGCTCTTCTTCCTTGTTGTTTCCTCCAGGATTATTCTAATCTAAGCTTGGATTGGCTTGTTTGGATTGATTGATCATTGATGAT
GTGTATTTCTCATCCAAAATCAATGAGTGACTTGTGCAATTATGGCCCAAGAAACAAAGAATTATCAAGCATCAAGTGACATGATGAGAATTATAAAATCCTCCTGGGATGATACATAGTATGCTAAAGATGGAGAAAATATTCTTGGAAGGATTACATTCCACCAATCAGAGCCTAGGTCATCTTGAATTAAGCAATTC
CTGTTACTGGGAATAAAGGTACATAGTAAGCTAAAGATGGAGATTCTCCTTGGAAGGATTCCTTTCCACCAATCAGTGCCTAGATCATCTTGAATTAAGCAATTCCTGTAACTGGGAATAATTTGTTTTCATTATTTCATGCTTTGGTTATTTGATTCCAACCGTTGATGTCTTTTTAAGTTTACCCTTATGGTTATTCT
AATCTAAATGGTAGCTCATATTTAGGGCTTGTGGC