sp|Q5QMN3|RH20_ORYSJ ----> DEAD-box ATP-dependent RNA helicase 20

Intron 1 GTTGTGGTTT ... TCTTTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.59	89.2	9.6	45
A2	32.94	110.4	6	54.4
A3	30.44	118.2	8.2	52
A4	23.38	92.6	3.2	28.2
A5	36.09	58.8	1.6	33.2
A6	29.87	386.4	0	165.2
A7	29.75	397	9	168.8
A8	30.51	284.2	11	125
A9	28.76	354.4	3.6	143.4
A10	26.65	323	0	117.8

>TRINITY_DN118460_c1_g1_i9 from sample A3 / Example spliced isoform

GCGAGTGATGGTCTATGTTATGTTTGCAACTTTATTATTGTTGGGGTCTATTGGAGGTTTTCTATTACACTATCTAAAACGCATGTCTTTCTAGGTAATAATTGGTTCATCAGACCTCAAGGCAAACCATGCAATTGAGCAAATTGTGGAGATTGTTTCTGAACAAGAGAAGTATACAAGGTTAATACATCTCTTAGAGG
AGATCATGGATGGTAGCCGCTTACTTATATTTTTGGAAACTAAGAAAGGCTGTGACCAAGTTACTAGGAAACTTCGTATGGATGGATGGCCAGCACTATCAATTCATGGAGATAAAAGCCAAGCCGAGAGAGACTGGGTCTTATCAGAATTTAAGGCTGGCAAGAGCCCTATTATGACTGCTACAGATGTCGCTGCACGG
GGCTTGGATGTTAAGGACATAAAATGTGTTATAAACTATGATTTTCCTGGCTCCATGGAGGATTATGTGCATCGTATTGGCCGTACTGGCCGAGCAGGTGCCAAGGGCAAGGCATATACATACTTCACTGCCGCAAATGCAAGGTTTGCCAAAGATCTGATAAAAATCCTTCAAGAAGCAGGGCAATCTATTAGCCCCAG
TTTGGCTGAAATGGGTCGTAGCTCTGCAGCTAGTGGAGGTTATGGAGGGTTTCGGGACCGTGGAAGAGGTTCTGGAAATCTACCATGGGGAAGATAGAGCTATTCCTGTCTTCTATGCAAATGATGGATGATAGGT

>TRINITY_DN118460_c1_g1_i1 from sample A3 / Example unspliced isoform:

AGGTAATAATTGGTTCATCAGACCTCAAGGCAAACCATGCAATTGAGCAAATTGTGGAGATTGTTTCTGAACAAGAGAAGTATACAAGgttgtggtttatgtgttttctactggctgtgttgtttatagcttttttgttgtgcaatgcacccatgaatatagacatttcttttccagGTTAATACATCTCTTAGAGGAGA
TCATGGATGGTAGCCGCTTACTTACCTTTTTGGAAACTAAGAAAGGTTGTCACCAAGTTACTAGGAAACTTCGTATGGATGGATGGCCAGCACTATCAATTCATGGAGATAAAAGCCAAGCCGAGAGAGACTGGGTCTTATCAGAATTTAAGGCTGGCAAGAGCCCTATTATGACTGCTACAGATGTCGCTGCACGGGGC
TTGGATGTTAAGGACATAAAATGTGTTATAAACTATGATTTTCCTGGCTCCATGGAGGATTATGTGCATCGTATTGGCCGTACTGGCCGAGCAGGTGCCAAGGGCAAGGCATATACATACTTCACTGCCGCAAATGCAAGGTTTGCCAAAGATCTGATAAAAATCCTTCAAGAAGCAGGGCAATCTATTAGCCCCAGTTT
GGCTGAAATGGGTCGTAGCTCTGCAGCTAGTGGAGGTTATGGAGGGTTTCGGGACCGTGGAAGAGGTTCTGGAAATCTACCATGGGGAAGATAGAGCTATTCCTGTCTTCTATGCAAATGATGGATGATAGGT

Intron 2 GTGAAATTAT ... GTGTTTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.37	34	19	31
A2	59.38	13	25	13
A3	38.6	35	11	33
A4	49.32	37	34	38
A5	86.67	1	11	2
A6	48.45	50	51	43
A7	47.25	48	42	44
A8	40.57	63	33	53
A9	41.7	65	53	40
A10	47.78	53	52	45

>TRINITY_DN48745_c2_g1_i1 from sample A7 / Example spliced isoform

ATTGCGTATCCATACGGCCAAATATTGGGTGTCTCTAATCCATTGATATTCATTATCTATCCGTTGAACTGGCTATTGGGTTTCTTCAATAAGGTAATAATTGGCTCACCAGACCTCAAGGAAAACCATGCAATTGAGCAAATTGTGGAGATTGTTTTTGAACATGAGAAGTATACAAGGTTAATACATCTCTTAGAGGA
GATCATGGATGGTAGCCGCTTACTTACCTTTTTGGAAACTAAGAAAGGTTGTCACCAAGTTACTAGGAAACTTCGTATGGATGGATGGCCAGCACTATCAATTCATGGAGATAAAAGCCAAGCCGAGAGAGACTGGGTCTTATCAGAATTTAAGGCTGGCAAGAGCCCTATTATGACTGCTACAGATGTCGCTGCACGGG
GCTTGGATGTTAAGGACATAAAATGTGTTATCAACTATGATTTTCCTGGCTCCATGGAGGATTATGTGCATCGTATTGGCCGTACTGGCCGAGCAGGTGCCAAGGGCAAGGCATATACATACTTCACTGCCGCAAATGCAAGGTTTGCCAAAGATCTGATAAAAATCCTTCAAGAAGCAGGGCAATCTATTAGCCCCAGT
TTGGCTGAAATGGGTCGTAGCTCTGCAGCTAGTGGAGGTTATGGAGGGTTTCGGGACCGTGGAAGAGTTTCTGGAAATCTGCGATGGGGAAGATAGAGTTGTTCCTGTCTTCTATGCAAATGATGGATGATAGGTTGTTGTGTCCTCTTAAGCGGTTCATTTGAATAATTAAATATTCTGTAACATAATAATTTTGAAAT
TTTTGGAATGAAAGACTTCATATCTAAAAAAAATGGAAGGGCATTTCCATGTCTACCATATTGTAAGAGAAAATGTAAAAATCAGGTAGAGACCACGACAATTTTTGTTGTTTCATATAATCTATAAGCATTATTTTTGTTGTATCAAAGACCTAAATTTTGGTGTAGTAGGATAAGCATTCTGCTCCCAACCCACCCAA
GTCAATGTATCTTAACAATTATTATTGGCAAAAGAACACT

>TRINITY_DN48745_c2_g1_i5 from sample A7 / Example unspliced isoform:

ATTGCGTATCCATACGGCCAAATATTGGGTGTCTCTAATCCATTGATATTCATTATCTATCCGTTGAACTGGCTATTGGGTTTCTTCAATAAGgtgaaattatttgtgcggctatccattcaggtcttgggttcttgtttagtctcaatctctgcataatccttacaaggggatttcttttcatctagtttgcatgtgag
aaaattaggatctgcttttctggggtggcaagtgacggtctatgttatgtttgcaactttattattgttgggtctattggaggttttctattacactatctaaaactcatgtgtttctagGTAATAATTGGCTCACCAGACCTCAAGGAAAACCATGCAATTGAGCAAATTGTGGAGATTGTTTTTGAACATGAGAAGTA
TACAAGGTTAATACATCTCTTAGAGGAGATCATGGATGGTAGCCGCTTACTTACCTTTTTGGAAACTAAGAAAGGTTGTCACCAAGTTACTAGGAAACTTCGTATGGATGGATGGCCAGCACTATCAATTCATGGAGATAAAAGCCAAGCCGAGAGAGACTGGGTCTTATCAGAATTTAAGGCTGGCAAGAGCCCTATTA
TGACTGCTACAGATGTCGCTGCACGGGGCTTGGATGTTAAGGACATAAAATGTGTTATCAACTATGATTTTCCTGGCTCCATGGAGGATTATGTGCATCGTATTGGCCGTACTGGCCGAGCAGGTGCCAAGGGCAAGGCATATACATACTTCACTGCCGCAAATGCAAGGTTTGCCAAAGATCTGATAAAAATCCTTCAA
GAAGCAGGGCAATCTATTAGCCCCAGTTTGGCTGAAATGGGTCGTAGCTCCGCAGCTAGTGGAGGTACAGTTGCAGGCCTCGCTCTATTGTTTTGACATAAGATTGGTGTTTCTTTTAATAATTCATCATTGGATAGATGGATGTTTAGCTGACTACATAATTCTCTGCTAGGTTATGGAGGGTTTCGGGACCGTGGAAG
AGTTTCTGGAAATCTGCGATGGGGAAGATAGAGTTGTTCCTGTCTTCTATGCAAATGATGGATGATAGGTTGTTGTGTCCTCTTAAGCGGTTCATTTGAATAATTAAATATTCTGTAACATAATAATTTTGAAATTTTTGGAATGAAAGACTTCATATCTAAAAAAAATGGAAGGGCATTTCCATGTCTACCATATTGTA
AGAGAAAATGTAAA

Intron 3 GTACAGTTGC ... TCTCTGCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.63	175.3	112.3	28.3
A2	26.1	131.7	69	24
A3	22.54	161	70	23.7
A4	25.43	100.7	51.7	17
A5	20.06	83	32.7	9
A6	20.56	747.3	220.7	166.3
A7	20.42	647	204.3	127.7
A8	20.1	767.3	187.3	198.7
A9	22.02	681.7	206	179
A10	20.06	437.7	126	93.7

>TRINITY_DN48745_c2_g1_i17 from sample A7 / Example spliced isoform

TTGATATGTTGGAGTCACAACATACGAACTTGCGAAGAGTGACATATCTGGTGTTAGACGAAGCTGATCGTATGCTCGACATGGGTTTTGAACCACAGATAAAGAAAATTGTTTCTCAAATTCGGCCAGATCGACAGACGTTATACTGGAGTGCAACCTGGCCAAAGGAAGTCGAACTTCTTGCCATGCAATCTCTGCAT
AATCCTTACAAGGTAATAATTGGTTCATCAGACCTCAAGGCAAACCATGCAATTGAGCAAATTGTGGAGATTGTTTCTGAACAAGAGAAGTATACAAGGTTAATACATCTCTTAGAGGAGATCATGGATGGTAGCCGCTTACTTATATTTTTGGAAACTAAGAAAGGCTGTGACCAAGTTACTAGGAAACTTCGTATGGA
TGGATGGCCAGCACTATCAATTCATGGAGATAAAAGCCAAGCCGAGAGAGACTGGGTCTTATCAGAATTTAAGGCTGGCAAGAGCCCTATTATGACTGCTACAGATGTCGCTGCACGGGGCTTGGATGTTAAGGACATAAAATGTGTTATCAACTATGATTTTCCTGGCTCCATGGAGGATTATGTGCATCGTATTGGCC
GTACTGGCCGAGCAGGTGCCAAGGGCAAGGCATATACATACTTCACTGCCGCAAATGCAAGGTTTGCCAAAGATCTGATAAAAATCCTTCAAGAAGCAGGGCAATCTATTAGCCCCAGTTTGGCTGAAATGGGTCGTAGCTCCGCAGCTAGTGGAGGTTATGGAGGGTTTCGGGACCGTGGAAGAGGTTCTGGAAATATG
CCATGGGGAAGATAGAGCTATTCCTGTCTTCTATGCAAATGATGGATGATAGGTCGTTGTGTCCTGTTAAGCGGTTCATTTAAATATTCTGTAACATAATAATTTTGAAATTAAGCGGTTCATTTATAGATCTGTATTTATTTGGGTGACTCATTATTTATGTTTGCAAATTTAGCAATGTTGTCGTTTGATGCATTTGA
TTGATTGCGTTATCATTATTTTTAATGCAATTGCATTCAATAATGTTAATTGGGATTTTAAATAGATTTATCCAAGTTCAAAAACCCATTACCTGACAGGACGAAAACACTATAATAATAC

>TRINITY_DN48745_c2_g1_i8 from sample A7 / Example unspliced isoform:

TTGATATGTTGGAGTCACAACATACGAACTTGCGAAGAGTGACATATCTGGTGTTAGACGAAGCTGATCGTATGCTCGACATGGGTTTTGAACCACAGATAAAGAAAATTGTTTCTCAAATTCGGCCAGATCGACAGACGTTATACTGGAGTGCAACCTGGCCAAAGGAAGTCGAACTTCTTGCCATGCAATCTCTGCAT
AATCCTTACAAGGTAATAATTGGTTCATCAGACCTCAAGGCAAACCATGCAATTGAGCAAATTGTGGAGATTGTTTCTGAACAAGAGAAGTATACAAGGTTAATACATCTCTTAGAGGAGATCATGGATGGTAGCCGCTTACTTATATTTTTGGAAACTAAGAAAGGCTGTGACCAAGTTACTAGGAAACTTCGTATGGA
TGGATGGCCAGCACTATCAATTCATGGAGATAAAAGCCAAGCCGAGAGAGACTGGGTCTTATCAGAATTTAAGGCTGGCAAGAGCCCTATTATGACTGCTACAGATGTCGCTGCACGGGGCTTGGATGTTAAGGACATAAAATGTGTTATCAACTATGATTTTCCTGGCTCCATGGAGGATTATGTGCATCGTATTGGCC
GTACTGGCCGAGCAGGTGCCAAGGGCAAGGCATATACATACTTCACTGCCGCAAATGCAAGGTTTGCCAAAGATCTGATAAAAATCCTTCAAGAAGCAGGGCAATCTATTAGCCCCAGTTTGGCTGAAATGGGTCGTAGCTCCGCAGCTAGTGGAGgtacagttgcaggcctcgctctattgttttgacataagattggt
gtttcttttaataattcatcattggatagatggatgtttagctgactacataattctctgctagGTTATGGAGGGTTTCGGGACCGTGGAAGAGGTTCTGGAAATATGCCATGGGGAAGATAGAGCTATTCCTGTCTTCTATGCAAATGATGGATGATAGGTCGTTGTGTCCTGTTAAGCGGTTCATTTAAATATTCTGT
AACATAATAATTTTGAAATTAAGCGGTTCATTTATAGATCTGTATTTATTTGGGTGACTCATTATTTATGTTTGCAAATTTAGCAATGTTGTCGTTTGATGCATTTGATTGATTGCGTTATCATTATTTTTAATGCAATTGCATTCAATAATGTTAATTGGGATTTTAAATAGATTTATCCAAGTTCAAAAACCCATTAC
CTGACAGGACGAAAACACTATAATAATAC