sp|Q2V0Z5|MIS12_ARATH ----> Protein MIS12 homolog

Intron 1 GTATACTATA ... TGCATTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.73	46.2	33	14.6
A2	40.81	46.5	32	5.2
A3	50.36	40.5	41	9.2
A4	37.9	26.7	16	10.3
A5	53.38	16.6	19	2.2
A6	33.35	96.2	48	9.9
A7	28.44	178.8	71	12.6
A8	32.01	142.9	67	23.8
A9	32.59	151.1	73	13.3
A10	32.59	147.3	71	14.5

>TRINITY_DN114906_c0_g1_i8 from sample A1 / Example spliced isoform

GTTTTTATTTAGATCTCGATCCTCAAGTCGATCTTACATTTGTAATAAGCGAGGAGAAGGAGATGGAGTCAGCAGCTGAAGGTGAAAGCAGGAGCAGAAGGGGAGAGGAAGGGGAGAGGCAAAGCGGGAGCAGAATAGCAGAAGGGGGGTTTGGTCCATCGTCGGAGGAAGGAAGTGACAGCAGCAGCATATTTGGGTCG
TTCAATTTCGATCCACAATTGTTCATAAACGATTTGCGAAATGCTGTCGACGATGTTATAGAAGAAGGATTTGATTTCTTTAAGGATCAAGCTCCAAAGGACTTAGGTGTCAAGGATGCTGATATATTAAACAATTTAGCTGAGGGTGTGGATGCTGTTCATCATGTTGTTCGGTTAAAACTTGACAAACAACTAGATAT
ATGGGAGAAGTACTGCCTTATGCATTGTTTTGCTAGTCCAGAGGGACTTGCATTGCCTGAAATGAAGAGTACCAAGGGAAGTGTTTTTGGATCATGCTATGCAGACAGATCAGCATATATGGATTTGGACACTCAACTTGAGTCTTTGCGTGACCAGCTTGTTGGTGTAGGAAGGGAATCAGCTGAGTTTCACAGAGAAC
TTTGTGTCCTAGAGAGGCAGACAGCAGTAAGCACACAATATGCAGAAGCCTTAGACGAGGCCCTACGCCCTTTGGAAGAGAATTCCACACATGATGTTATGAAAGCAGAGATTCTGAGAATAGCTTCAGAGCTTCATGAGAAGATGGAAAAGTTGAAGACTGAAAGAAGGGGAAAAATGGAAAAGTCACGCCTTGAGAGG
ATGAGAAGCCTGAACCTAACTCACACCAATAAAAAAGAAATTCAAGGTTTCGAAGATAGTCTGGAAGACTTGCAACAATTTTTAAACAATTTCGAGGTTCTGAATCAAAGCTAACATATAATGATTTGGGGAGAATGGCTGTCTTCAGTGCGCTCTGTAGCTTCTTAGTCTACTCAGGCTAACAATCAGGAACAATGTAA
TGAATATGCATACTTAACAGATGGCACAAGTTTCACACTAAATAGCTTTGTAATAATCTTTATGCTTGAAGTCTCTCTTCGATATTGGAATCGTAAGATGATCAAACTATTAAACTTTTATAC

>TRINITY_DN114906_c0_g1_i5 from sample A1 / Example unspliced isoform:

GTTTTTATTTAGATCTCGATCCTCAAGTCGATCTTACATTTGTAATAAGCGAGGAGAAGGAGATGGAGTCAGCAGCTGAAGGTGAAAGCAGGAGCAGAAGGGGAGAGGAAGGGGAGAGGCAAAGCGGGAGCAGAATAGCAGAAGGGGGGTTTGGTCCATCGTCGGAGGAAGGAAGTGACAGCAGCAGCATATTTGGGTCG
TTCAATTTCGATCCACAATTGTTCATAAACGATTTGCGAAATGCTGTCGACGATGTTATAGAAGAAGGATTTGATTTCTTTAAGGAgtatactatacacggcttgctttctttaccattgttcatattatctttaaagctcttccctagcatatttattttcagaaattcatctggtaggtattaacagggcttcaaaaa
aaataatttatggtgtattgcattacagTCAAGCTCCAAAGGACTTAGGTGTCAAGGATGCTGATATATTAAACAATTTAGCTGAGGGTGTGGATGCTGTTCATCATGTTGTTCGGTTAAAACTTGACAAACAACTAGATATATGGGAGAAGTACTGCCTTATGCATTGTTTTGCTAGTCCAGAGGGACTTGCATTGCCT
GAAATGAAGAGTACCAAGGGAAGTGTTTTTGGATCATGCTATGCAGACAGATCAGCATATATGGATTTGGACACTCAACTTGAGTCTTTGCGTGACCAGCTTGTTGGTGTAGGAAGGGAATCAGCTGAGTTTCACAGAGAACTTTGTGTCCTAGAGAGGCAGACAGCAGTAAGCACACAATATGCAGAAGCCTTAGACGA
GGCCCTACGCCCTTTGGAAGAGAATTCCACACATGATGTTATGAAAGCAGAGATTCTGAGAATAGCTTCAGAGCTTCATGAGAAGATGGAAAAGTTGAAGACTGAAAGAAGGGGAAAAATGGAAAAGTCACGCCTTGAGAGGATGAGAAGCCTGAACCTAACTCACACCAATAAAAAAGAAATTCAAGGTTTCGAAGATA
GTCTGGAAGACTTGCAACAATTTTTAAACAATTTCGAGGTTCTGAATCAAAGCTAACATATAATGATTTGGGGAGAATGGCTGTCTTCAGTGCGCTCTGTAGCTTCTTAGTCTACTCAGGCTAACAATCAGGAACAATGTAATGAATATGCATACTTAACAGATGGCACAAGTTTCACACTAAATAGCTTTGTAATAATC
TTTATGCTTGAAGTCTCTCTTCGATATTGGAATCGTAAGATGATCAAACTATTAAACTTTTATAC

Intron 2 GTGAGTGAGT ... CATGTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.59	21	7	8
A2	12.5	21	0	3
A3	22.22	21	0	6
A4	25.93	20	1	7
A5	0	10	0	0
A6	8.82	62	0	6
A7	14.47	130	0	22
A8	17.42	109	0	23
A9	14.6	117	2	20
A10	8.79	83	0	8

>TRINITY_DN114906_c0_g1_i8 from sample A1 / Example spliced isoform

GTTTTTATTTAGATCTCGATCCTCAAGTCGATCTTACATTTGTAATAAGCGAGGAGAAGGAGATGGAGTCAGCAGCTGAAGGTGAAAGCAGGAGCAGAAGGGGAGAGGAAGGGGAGAGGCAAAGCGGGAGCAGAATAGCAGAAGGGGGGTTTGGTCCATCGTCGGAGGAAGGAAGTGACAGCAGCAGCATATTTGGGTCG
TTCAATTTCGATCCACAATTGTTCATAAACGATTTGCGAAATGCTGTCGACGATGTTATAGAAGAAGGATTTGATTTCTTTAAGGATCAAGCTCCAAAGGACTTAGGTGTCAAGGATGCTGATATATTAAACAATTTAGCTGAGGGTGTGGATGCTGTTCATCATGTTGTTCGGTTAAAACTTGACAAACAACTAGATAT
ATGGGAGAAGTACTGCCTTATGCATTGTTTTGCTAGTCCAGAGGGACTTGCATTGCCTGAAATGAAGAGTACCAAGGGAAGTGTTTTTGGATCATGCTATGCAGACAGATCAGCATATATGGATTTGGACACTCAACTTGAGTCTTTGCGTGACCAGCTTGTTGGTGTAGGAAGGGAATCAGCTGAGTTTCACAGAGAAC
TTTGTGTCCTAGAGAGGCAGACAGCAGTAAGCACACAATATGCAGAAGCCTTAGACGAGGCCCTACGCCCTTTGGAAGAGAATTCCACACATGATGTTATGAAAGCAGAGATTCTGAGAATAGCTTCAGAGCTTCATGAGAAGATGGAAAAGTTGAAGACTGAAAGAAGGGGAAAAATGGAAAAGTCACGCCTTGAGAGG
ATGAGAAGCCTGAACCTAACTCACACCAATAAAAAAGAAATTCAAGGTTTCGAAGATAGTCTGGAAGACTTGCAACAATTTTTAAACAATTTCGAGGTTCTGAATCAAAGCTAACATATAATGATTTGGGGAGAATGGCTGTCTTCAGTGCGCTCTGTAGCTTCTTAGTCTACTCAGGCTAACAATCAGGAACAATGTAA
TGAATATGCATACTTAACAGATGGCACAAGTTTCACACTAAATAGCTTTGTAATAATCTTTATGCTTGAAGTCTCTCTTCGATATTGGAATCGTAAGATGATCAAACTATTAAACTTTTATAC

>TRINITY_DN114906_c0_g1_i4 from sample A1 / Example unspliced isoform:

AAGAGTACCAAGGGAAGTGTTTTTGGATCATGCTATGCAGACAGATCAGCATATATGGATTTGGACACTCAACTTGAGTCTTTGCGTGACCAGCTTGTTGGTgtgagtgagtgcgattcttcttaccattcataattatattcagagtgattatgttaattatattttccaaacagtactttgactcatggccttttctg
ggttagaaatggaacaataccatgcagatatccagtggctcttttgcaataattatatggttgtgagattcctgcattttgcttttaaaaatattgctgaaatagggatgattaacatgtatcagGTAGGAAGGGAATCAGCTGAGTTTCACAGAGAACTTTGTGTCCTAGAGAGGCAGACAGCAGTAAGCACACAATAT
GCAGAAGCCTTAGACGAGGCCCTACGCCCTTTGGAAGAGAATTCCACACATGATGTTATGAAAGGTGATGCTCTCTTGGTGTTTGTGCCATCGTGGAAGACTAAGCAAACCATGATACATCCTGTTAATAATTAACATTGATGATTATTAACATTTTAGAGGTGAACTAGAAAAATGTCTTAATTATGACATTGTAAGAT
TCCAATTAGTAGAATTTGCCTTCTTACTGTGTTACTTCCA

Intron 3 GTCAAGTACG ... AACAAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.81	17.8	10	3
A2	14.29	18	2	4
A3	26.98	23	13	4
A4	22.31	5.3	1	2
A5	20	4	0	2
A6	19.5	64	19	12
A7	23.43	116	39	32
A8	19.57	189	57	35
A9	19.2	141	37	30
A10	18.67	98	27	18

>TRINITY_DN43005_c14_g1_i3 from sample A7 / Example spliced isoform

AGAGATTCTGAGAATAGCTTCAGAGCTTCATGAGAAGATGGAAAAGTTGAAGACTGAAAGAAGGGGAAAAATGGAAAAGTCACGCCTTGAGAGGATGAGAAGCCTGAACCTAACTCACACCAATAAAAAAGAAATTCAAGGTTTCGAAGATAGTCTGGAAGACTTGCAACAATTTTTAAACAATTTCGAGGTTCTGAATC
AAAGCTAACATATAATGATTTGGGGAGAATGGCTGTCTTCAGTGCGCTCTGTAGCTTCTTAGTCTACTCAGGCTAACAATCAGGAACAATGTAATGAATATGCATACTTAACAGATGGCACAATTTAGCTGAGGGTGTGGATGCTGTTCATCATGTTGTTCGGTTAAAACTTGACAAACAACTAGATATATGGGAGAAGT
ACTGCCTTATGCATTGTTTTGCTAGTCCAGAGGGACTTGCATTGCCTGAAATGAAGAGTACCAAGGGAAGTGTTTTTGGATCATGCTATGCAGACAGATCAGCATATATGGATTTGGACACTCAACTTGAGTCTTTGCGTGACCAGCTTGTTGGTGTAGGAAGGGAATCAGCTGAGTTTCACAGAGAACTTTGTGTCCTA
GAGAGGCAGACAGCAGTAAGCACACAATATGCAGAAGCCTTAGACGAGGCCCTACGCCCTTTGGAAGAGAATTCCACACATGATGTTATGAAAGAGATTCTGAGAATAGCTTCAGAGCTTCATGAGAAGATGGAAAAGTTGAAGACTGAAAGAAGGGGAAAAATGGAAAAGTCACGCCTTGAGAGGATGAGAAGCCTGAA
CCTAACTCACACCAATAAAAAAGAAATTCAAGGTTTCGAAGATAGTCTGGAAGACTTGCAACAATTTTTAAACAATTTCGAGGTTCTGAATCAAAGCTAACATATAATGATTTGGGGAGAATGGCTGTCTTCAGTGCGCTCTGTAGCTTCTTAGTCTACTCAGGCTAACAATCAGGAACAATGTAATGAATATGCATACT
TAACAGATGGCACAAGTTTCACACTAAATAGCTTTGTAATAATCTTTATGCTTGAAGTCTCTCTTCGATATTGGAATC

>TRINITY_DN43005_c14_g1_i2 from sample A7 / Example unspliced isoform:

AGGGTGTGGATGCTGTTCATCATGTTGTTCGGTTAAAACTTGACAAACAACTAGATATATGGGAGAAGTACTGCCTTATGCATTGTTTTGCTAGTCCAGAGGGACTTGCATTGCCTGAAATGAAGAGTACCAAGGGAAGTGTTTTTGGATCATGCTATGCAGACAGATCAGCATATATGGATTTGGACACTCAACTTGAG
TCTTTGCGTGACCAGCTTGTTGGTGTAGGAAGGGAATCAGCTGAGTTTCACAGAGAACTTTGTGTCCTAGAGAGGCAGACAGCAGTAAGCACACAATATGCAGAAGCCTTAGACGAGGCCCTACGCCCTTTGGAAGAGAATTCCACACATGATGTTATGAAAGgtcaagtacgacgcagatggcacacttggcaagcaca
aggcactgttggtagaaaagggtttctccctacgaaagggcattgattatgttgtgaccttttctcctacaacaaagcagAGATTCTGAGAATAGCTTCAGAGCTTCATGAGAAGATGGAAAAGTTGAAGACTGAAAGAAGGGGAAAAATGGAAAAGTCACGCCTTGAGAGGATGAGAAGCCTGAACCTAACTCACACCA
ATAAAAAAGAAATTCAAGGTTTCGAAGATAGTCTGGAAGACTTGCAACAATTTTTAAACAATTTCGAGGTTCTGAATCAAAGCTAACATATAATGATTTGGGGAGAATGGCTGTCTTCAGTGCGCTCTGTAGCTTCTTAGTCTACTCAGGCTAACAATCAGGAACAATGTAATGAATATGCATACTTAACAGATGGCACA
AGTTTCACACTAAATAGCTTTGTAATAATCTTTATGCTTGAAGTCTCTCTTCGATATTGGAATCGTAAGATGATCAAACTATTAAACTTTTATACTGACATCTTTATTATACTATATAGCTATCTATTTATTTCAAGTTGAATTTATAGTAATTTTGATGTGTTTTGAATTTTTTATTAGATATAGCTTGGGTGGTTGGG
AGGAATGATTGTTACTCTCCTTACCTAGGTACAATTCTTATAAGTGACATCATGCTCTTGTTGAGCCTTTATGTATCGACTAGTTTTCCTTTATTCCTTTGGAATAGCAGAAAGGTGATTTTTCGGCTGAAAAAAATGTAATGTTTTGAATATTAAATATAATTTTTGAAAAATAAAAATTATTTTCAAATTATTAAAAT
TGAA

Intron 4 GTAAGATGAT ... TTTTTATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	2	2	0
A2	80	1	8	0
A3	55.56	4	7	3
A4	100	0	3	4
A5	-1	0	0	0
A6	53.85	21	41	8
A7	58.28	34	71	24
A8	58.1	44	99	23
A9	53.64	51	80	38
A10	68.42	12	36	16

>TRINITY_DN43005_c14_g1_i4 from sample A7 / Example spliced isoform

AGATTCTGAGAATAGCTTCAGAGCTTCATGAGAAGATGGAAAAGTTGAAGACTGAAAGAAGGGGAAAAATGGAAAAGTCACGCCTTGAGAGGATGAGAAGCCTGAACCTAACTCACACCAATAAAAAAGAAATTCAAGGTTTCGAAGATAGTCTGGAAGACTTGCAACAATTTTTAAACAATTTCGAGGTTCTGAATCAA
AGCTAACATATAATGATTTGGGGAGAATGGCTGTCTTCAGTGCGCTCTGTAGCTTCTTAGTCTACTCAGGCTAACAATCAGGAACAATGTAATGAATATGCATACTTAACAGATGGCACAAGTTTCACACTAAATAGCTTTGTAATAATCTTTATGCTTGAAGTCTCTCTTCGATATTGGAATCATATAGCTTGGGTGGT
TGGGAGGAATGATTGTTACTCTCCTTACCTAGGTACAATTCTTATAAGTGACATCATGCTCTTGTTGAGCCTTTATGTATCGACTAGTTTTCCTTTATTCCTTTGGAATAGCAGAAAGGTGATTTTTCGGCTGAAAAAAATGTAATGTTTTGAATATTAAATATAATTTTTGAAAAATAAAAATTATTTTCAAATTATTA
AAATTGAA

>TRINITY_DN43005_c14_g1_i6 from sample A7 / Example unspliced isoform:

TTTTGAATTGTACCCGCTCTTATTAATGCCCGTGTCTATTTAATATATCTCTATCGCTACCACTGGCGTTCCGTGTTTTTATTTAGATCTCGATCCTCAAGTCGATCTTACATTTGTAATAAGCGAGGAGAAGGAGATGGAGTCAGCAGCTGAAGGTGAAAGCAGGAGCAGAAGGGGAGAGGAAGGGGAGAGGCAAAGCG
GGAGCAGAATAGCAGAAGGGGGGTTTGGTCCATCGTCGGAGGAAGGAAGTGACAGCAGCAGCATATTTGGGTCGTTCAATTTCGATCCACAATTGTTCATAAACGATTTGCGAAATGCTGTCGACGATGTTATAGAAGAAGGATTTGATTTCTTTAAGGAGTATACTATACACGGCTTGCTTTCTTTACCATTGTTCATA
TTATCTTTAAAGCTCTTCCCTAGCATATTTATTTTCAGAAATTCATCTGGTAGGTATTAACAGGGCTTCAAAAAAAATAATTTATGGTGTATTGCATTACAGTCAAGCTCCAAAGGACTTAGGTGTCAAGGATGCTGATATATTAAACAATTTAGCTGAGGGTGTGGATGCTGTTCATCATGTTGTTCGGTTAAAACTTG
ACAAACAACTAGATATATGGGAGAAGTACTGCCTTATGCATTGTTTTGCTAGTCCAGAGGGACTTGCATTGCCTGAAATGAAGAGTACCAAGGGAAGTGTTTTTGGATCATGCTATGCAGACAGATCAGCATATATGGATTTGGACACTCAACTTGAGTCTTTGCGTGACCAGCTTGTTGGTGTAGGAAGGGAATCAGCT
GAGTTTCACAGAGAACTTTGTGTCCTAGAGAGGCAGACAGCAGTAAGCACACAATATGCAGAAGCCTTAGACGAGGCCCTACGCCCTTTGGAAGAGAATTCCACACATGATGTTATGAAAGAGATTCTGAGAATAGCTTCAGAGCTTCATGAGAAGATGGAAAAGTTGAAGACTGAAAGAAGGGGAAAAATGGAAAAGTC
ACGCCTTGAGAGGATGAGAAGCCTGAACCTAACTCACACCAATAAAAAAGAAATTCAAGGTTTCGAAGATAGTCTGGAAGACTTGCAACAATTTTTAAACAATTTCGAGGTTCTGAATCAAAGCTAACATATAATGATTTGGGGAGAATGGCTGTCTTCAGTGCGCTCTGTAGCTTCTTAGTCTACTCAGGCTAACAATC
AGGAACAATGTAATGAATATGCATACTTAACAGATGGCACAAGTTTCACACTAAATAGCTTTGTAATAATCTTTATGCTTGAAGTCTCTCTTCGATATTGGAATCgtaagatgatcaaactattaaacttttatactgacatctttattatactatatagctatctatttatttcaagttgaatttatagtaattttgat
gtgttttgaattttttattagATATAGCTTGGGTGGTTGGGAGGAATGATTGTTACTCTCCTTACCTAGGTACAATTCTTATAAGTGACATCATGCTCTTGTTGAGCCTTTATGTATCGACTAGTTTTCCTTTATTCCTTTGGAATAGCAGAAAGGTGATTTTTCGGCTGAAAAAAATGTAATGTTTTGAATATTAAATA
TAATTTTTGAAAAATAAAAATTATTTTCAAATTATTAAAATTGAA