sp|Q9C7U5|E132_ARATH ----> Glucan endo-1,3-beta-glucosidase 2

Intron 1 GCAAGTGAAC ... CTGGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.23	4	14	4
A2	65.22	4	6	9
A3	55.99	9.8	12	13
A4	56.76	8	6	15
A5	83.33	1	5	5
A6	73.29	21	52	63.3
A7	60.62	44.8	72	66
A8	57.17	37.8	48	53
A9	62.74	49	86	79
A10	68.7	34.2	81	69

>TRINITY_DN98931_c0_g1_i1 from sample A1 / Example spliced isoform

GCCAGGGCTCCTCCGTCGATGTGAAGCTGACGAGTTAATGCAGCCCGGGGGTTGGTGAAAGCAACACCCTCCAGGTTGGCAAGAGACACCCATCGCTGTAAACTCCATGAACCGGTATCCTGGTCCGATCTCCTGCAACTCCCATCATTGCGACCTCCGCGAAGACGAAGACTCCACAACCGGGTAGTGTGCTAGAACAC
TGCGTTTAACCCACTCATCAGCATTCAGTATGCTAGATGCAACGGTCAGAATCTCCTCATTCTTCACAGCTATTACAACTGAGAGACCTGTGTTGGCCTTTCACATAGAAAGCGCAGCTACAACAACAATCCCTAGTTTTCTGAATTTGGTATACCGTCCATTTCAATTTTCAATATGGATTTTCAATCTCATTGTTTAT
GAGCTCTCAAACCTTGTTTATCCATATCCCTGTAATGGCAACCATGGAAGAAGCAAAAAAAAATGCTTGATCTGAATTTATTTTTATTTTTGGGGGTTTGTTTGGATTTGCATTTAGGGTTGACAATTTAAGGTGGCGTTAACAAGAATGGTTGTTGATTTACCTCCTGCCTTTGGATGGGTCGTCTTGGCTGCTATTGT
ATACTTCGTGCTCAATATTTGGATGGCCGTTCAGGTCGGAAAGGCTAGAAAATGGTATTTATAATTATATCACAATTGCTTACGCTCTTTCTTAGTAGAAATACAAATCTAAAATGCGAATATTATGAAGGATGAAATATTTTGAGGTTATTGTGGTTTGAAAAATATCTATACTTTATATACTATCAATCCATATACAT
GAACTTATTTCAGATTGCAATATACTACCAATTCATACCGTTGAATATGGACTACTTATTTCAAATTGCAATATACTACCA

>TRINITY_DN98931_c0_g1_i2 from sample A1 / Example unspliced isoform:

GCCAGGGCTCCTCCGTCGATGTGAAGCTGACGAGTTAATGCAGCCCGGGGGTTGGTGAAAGCAACACCCTCCAGGTTGGCAAGAGACACCCATCGCTGTAAACTCCATGAACCGGTATCCTGGTCCGATCTCCTGCAACTCCCATCATTGCGACCTCCGCGAAGACGAAGACTCCACAACCGgcaagtgaactgtgaatg
ttctggatagcacgaactaaattagaccatctatcctgattattgtagttggtgaggattttgtttccaacagttatggcgttaattctggttgcagGGTAGTGTGCTAGAACACTGCGTTTAACCCACTCATCAACATTCAGTATGCTAGATGCAACAGTTAGAATCTCCTCATTCTTCACAGCTATTACAACTGAGAG
ACCTGTGTTGGCCTTTCACATAGAAAGCGCAGCTACAACAACAATCCCTAGTTTTCTGAATTTGGTATACCGTCCATTTCAATTTTCAATATGGATTTTCAATCTCATTGTTTATGAGCTCTCAAACCTTGTTTATCCATATCCCTGTAATGGCAACCATGGAAGAAGCAAAAAAAAATGCTTGATCTGAATTTATTTTT
ATTTTTGGGGGTTTGTTTGGATTTGCATTTAGGGTTGACAATTTAAGGTGGCGTTAACAAGAATGGTTGTTGATTTACCTCCTGCCTTTGGATGGGTCGTCTTGGCTGCTATTGTATACTTCGTGCTCAATATTTGGATGGCCGTTCAGGTCGGAAAGGCTAGAAAATGGTATTTATAATTATATCACAATTGCTTACGC
TCTTTCTTAGTAGAAATACAAATCTAAAATGCGAATATTATGAAGGATGAAATATTTTGAGGTTATTGTGGTTTGAAAAATATCTATACTTTATATACTATCAATCCATATACATGAACTTATTTCAGATTGCAATATACTACCAATTCATACCGTTGAATATGGACTACTTATTTCAAATTGCAATATACTACCA

Intron 2 GTATACCGTC ... TTGCATTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.92	0.4	3	4
A2	83.84	0.7	3	2
A3	92.8	0.6	7	7
A4	81.82	0.9	4	4
A5	100	0	2	0
A6	77.23	15.8	58.7	44
A7	75.09	16.2	54.6	39
A8	82.36	15.5	84	54
A9	81.34	14.2	73.9	39
A10	84.18	8.5	46	42

>TRINITY_DN39470_c1_g1_i3 from sample A6 / Example spliced isoform

CACTCCGACACTTCGGCACCGTCCCCATCCCCCTCCGCTAGATCGCCAGTCTAACACTCCTTCCACACGAATCTAGATTGCGAATCTCGAAGTGAGCCAGGGCTCCTCCGTCGATGTGAAGCTGACGAGTTAATGCAGCCCGGGGGTTGGTGAAAGCAACACCCTCCAGGTTGGCAAGAGACACCCATCGCTGTAAACTC
CATGAACCGGTATCCTGGTCCGATCTCCTGCAACTCCCATCATTGCGACCTCCGCGAAGACGAAGACTCCACAACCGGCAAGTGAACTGTGAATGTTCTGGATAGCACGAACTAAATTAGACCATCTATCCTGATTATTGTAGTTGGTGAGGATTTTGTTTCCAACAGTTATGGCGTTAATTCTGGTTGCAGGGTAGTGT
GCTAGAACACTGCGTTTAACCCACTCATCAGCATTCAGTATGCTAGATGCAACGGTCAGAATCTCCTCATTCTTCACAGCTATTACAACTGAGAGACCCGTGTTGGCCTTTCACATAGAAAGCGCAGCGACAACAAGAATCCCCAGTTTTCTGAATTTGGGTTGACAATTTAAGGTGGC

>TRINITY_DN39470_c1_g1_i1 from sample A6 / Example unspliced isoform:

CACTCCGACACTTCGGCACCGTCCCCATCCCCCTCCGCTAGATCGCCAGTCTAACACTCCTTCCACACGAATCTAGATTGCGAATCTCGAAGTGAGCCAGGGCTCCTCCGTCGATGTGAAGCTGACGAGTTAATGCAGCCCGGGGGTTGGTGAAAGCAACACCCTCCAGGTTGGCAAGAGACACCCATCGCTGTAAACTC
CATGAACCGGTATCCTGGTCCGATCTCCTGCAACTCCCATCATTGCGACCTCCGCGAAGACGAAGACTCCACAACCGGCAAGTGAACTGTGAATGTTCTGGATAGCACGAACTAAATTAGACCATCTATCCTGATTATTGTAGTTGGTGAGGATTTTGTTTCCAACAGTTATGGCGTTAATTCTGGTTGCAGGGTAGTGT
GCTAGAACACTGCGTTTAACCCACTCATCAGCATTCAGTATGCTAGATGCAACGGTCAGAATCTCCTCATTCTTCACAGCTATTACAACTGAGAGACCCGTGTTGGCCTTTCACATAGAAAGCGCAGCGACAACAAGAATCCCCAGTTTTCTGAATTTGgtataccgtccatttcaattttcaatatggattttcaatct
cattgtttatgagctctcaaaccttgtttatccatatccctgtaatggcaaccatggaagaagcaaaaaaaaatgcttgatctgaatttatttttatttttgggggtttgtttggatttgcatttagGGTTGACAATTTAAGGTGGCGTTAACAAGAATGGTTGTTGATTTACCTCCTGCCTTTGGATGGGTCGTCTTGG
CTGCTATTGTATACTTCGTGCTCAATATTTGGATGGCCGTTCAGGTCGGAAAGGCTAGAAAATGGTATTTATAATTATATCACAATTGCTTACGCTCTTTCTTAGTAGAAATACAAATCTAAAATGCGAATATTATGAAGGATGAAATATTTTGAGGTTATCGTGGTTTGAAAAATATCTATACTTTATATATTATCAAT
CCATATACATGAAATTATTTCAGATTGCAATATACTACCAATTCATACCCTTGAATATGGACTACTTATTTCAAATTGCAATATACTATCAATCTATATACAAATTCTGTTGAATACGAATTTATTTCAAAGTGTATAAAATATGAAATATAGGGATATTTTT