sp|Q9FQ08|KU70_ARATH ----> ATP-dependent DNA helicase 2 subunit KU70

Intron 1 GTTGGCTTTT ... GATTCATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.08	30	12	5
A2	33.33	21	11	10
A3	24.14	33	7	14
A4	20.93	17	5	4
A5	25	6	4	0
A6	27.66	17	6	7
A7	25.84	66	8	38
A8	26.98	46	7	27
A9	22.95	47	13	15
A10	25.53	35	10	14

>TRINITY_DN116711_c1_g1_i13 from sample A2 / Example spliced isoform

GTGAGTTACAAAGAATTTTACATGTTAATGCAAACTATCTCTCTGCTGTTTTTAATTATGAATCCTCCTTCTGCATCTTCAGGATCAATCACTTGGCTTGATTCTGTGACCAACATTCCCTTGAAGTCAGAAAGCTCTTATATTTGTGCAGACACTGGTGCCTTGCTCACAGAGCCATTACCACGGTTCCATATGTATAA
GAATGAAAAGGTTAAACTCACTATTGATGAACTATCTGAAATAAAAAAGGTCACTAGAGTGCCTTTGCGACTTTTAGGATTTAAGCCATTACATTGTTTGAAAGATTACCATAATTTGCGGCCAGCAACTTTTCTCTACCCAAGTGATGAGGAGATTAGTGGAAGTACATGTGCATTTATTGCTCTCTATAGGGCAATGT
TGCGTTTGCAAAAATATGCTGTGGCATTTTATGGAAAAACATCAATTCCGGAACTGGTTGCTCTAGTTGCACGGGAAGAAATAACAAGTTCAAGTGGCCAAATTGAACCTCCTGGGATGCATATGATCTATCTGCCTTATTCAGATGATATTCGTCATGTTGAAAATCTTCATATGAGTGCAAATGGTCCAGCACCTCGA
GCATCAGAGGAACAAATAGATAGGGCTATATCTATGATGAGAAAGTTAGAATTAAAGGACTTTTCTGTTTATCAAATCTCAAACCCTGCTTTGCAGAGGCATTATGCAATATTGCAAGTTTTAGCCCTTGAAGAAGATGATTTGCCAGAGTCAAAAGATGAGACCATGCCAGATGAAGAGGGCATGCAAAGACCAGGAAT
CACGAAAGCTGTACAGGCATTTAAAGATGCTGTATATGGTGAAAATCATGACTTTGAGGAAGCTGAGGCAGAGGCAACTAAAGCCAAAGGCAGTGAAGCATCTCAAAAGAGAAAAGCATCAGCTGAAGTAGCAGCAAAGGAGGCAGCGAGTTATAATTGGAGTGAACTTGCAGACACGGGAAAG

>TRINITY_DN116711_c1_g1_i2 from sample A2 / Example unspliced isoform:

TGCAGTTTATAAATTTGGCCCTAAATTTCTCTGGTGTTTGGCGCCCTTTTATACACCTCCAGAATCCTCTGAGTCCCGTTTTACCTTTCAAGCTGACGATATTGCGCAGAAGGAAGTGATACCGCCGTGGTACAGACGGGCTCGTTTCTAGATTTTGTTCAGTTGGATGATCAAGCTTAACCGATGGATTGGGATTCAAA
TGACTTGTTCCAAGATGATGAGGAAGATCAGGATAATACCTATACACAGGACCGAGAAGCCAACAAGGAGTTTGTTATTTACATGATAGATGCTGGGCCTGATATGTTTTTCCAAATAAAACAAGAGGATGAAGGGAAGACAGAAACACATTTCAGTACTGTGGTCAAGTGTATTGTTGAATCTCTGAAGATTCGCATCA
TCAACAAAGACTCTGATGAAGTTGCAATATGTTTCTATAACACTAGAGAGAAGAAAAACATGCAGGAATCTGAGGGTGTCTATGTTTTCAATGTTTCTGAAAGAGAAGAACTTGACCGTCCAACAGCACGATTGATCAAAGACTTCAGCCACATTGAAGATAAGTTCAAAAGGGAAATTGGGAGTCAAAGCGGAATTATT
CCTGGATCAAGAGAAAATCCATTATACAATGCTCTTTGGGTTGCACAGGGACTCTTACGCAAGGGGTCCACTCGCAGTGCTGACAAGCGTATACTATTATTTACCAATAATGATGATCCTTTTGGAAATGTCGATGGTGGTGCCAAAGCAGACATGAGGAGGACAACAATTCAGCGAGCCAAAGATGCACAAGATTTAGG
TATCTCAATTGAGCTTTTCCCCTTGAATAGGCCAGGCGAAGAATTCAATGTTTCCATTTTCTATTCTGATATGCTTGAGTTGGAGAAGGAGGAAATATCTCAGTTCATGCCGATGGCAGTTGCAAAGTTTGAAGATCTTAAGGACCAATTAAGGAAAAGGATGTACAAGAAGCGTACCGTTCGAAAGATTACTTTAGTAT
TAGCTGATGGGATGTCTATTGGGTTGCATACATATGCTTTAATTCGGCCTGCAATACCAGGATCAATCACTTGGCTTGATTCTGTGACCAACATTCCCTTGAAGTCAGAAAGCTCTTATATTTGTGCAGACACTGGTGCCTTGCTCACAGAGCCATTACCACGGTTCCATATGTATAAGAATGAAAAGGTTAAACTCACT
ATTGATGAACTATCTGAAATAAAAAAGGTCACTAGAGTGCCTTTGCGACTTTTAGGATTTAAGCCATTACATTGTTTGAAAGATTACCATAATTTGCGGCCAGCAACTTTTCTCTACCCAAGTGATGAGGAGATTAGTGGAAGTACATGTGCATTTATTGCTCTCTATAGGGCAATGTTGCGTTTGCAAAAATATGCTGT
GGCATTTTATGGAAAAACATCAATTCCGGAACTGGTTGCTCTAGTTGCACGGgttggcttttcctttcacgttgtaagatgattcatcagGAAGAAATAACAAGTTCAAGTGGCCAAATTGAACCTCCTGGGATGCATATGATCTATCTGCCTTATTCAGATGATATTCGTCATGTTGAAAATCTTCATATGAGTGCAAA
TGGTCCAGCACCTCGAGCATCAGAGGAACAAATAGATAGGGCTATATCTATGATGAGAAAGTTAGAATTAAAGGACTTTTCTGTTTATCAAATCTCAAACCCTGCTTTGCAGAGGCATTATGCAATATTGCAAGTTTTAGCCCTTGAAGAAGATGATTTGCCAGAGTCAAAAGATGAGACCATGCCAGATGAAGAGGGCA
TGCAAAGACCAGGAATCACGAAAGCTGTACAGGCATTTAAAGATGCTGTATATGGTGAAAATCATGACTTTGAGGAAGCTGAGGCAGAGGCAACTAAAGCCAAAGGCAGTGAAGCATCTCAAAAGAGAAAAGCATCAGCTGAAGTAGCAGCAAAGGAGGCAGCGAGTTATAATTGGAGTGAACTTGCAGACACGGGAAAG
CTGAAGGACTTAACCGTGGTTGAACTAAAGTATTATTTGACTGCTCACAATCTGCCAGTTACTGGCAAAAAGGAAGTCTTGATCAACAGAATCCTGACACATTTGGGAAAGTGAAGTCTTATGATGTGACCATGATTAAGTTTGTACAGTCGTTCATGTTCATCAGTGAACTAGACCATATGAATACTTCTCCAAAATTT
CCAAGGGAGCATTTGACTTCCGGAACTCTTACACCAATTTTGGGACCATATCCAAGTCCAGGTGGTTTCATTGAACTATATGGGGACTCGCTTCTTTCAAATATGCACATGTACGATTTTGTAAGAAAGCTTTGGGATGTTATTCACTGAGCATAATGATTATCTGTATCACAAAAGTTTTTTATCTTGTAAC

Intron 2 GTATAGTAGA ... GCATCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.95	47	4	24
A2	12.5	28	4	4
A3	24.44	17	7	4
A4	28.57	20	6	10
A5	23.81	8	3	2
A6	23.26	33	6	14
A7	17.99	57	2	23
A8	20.79	40	7	14
A9	14.48	62	12	9
A10	19.54	35	9	8

>TRINITY_DN108810_c0_g1_i6 from sample A4 / Example spliced isoform

GTTCATGAGAAAAGTATCCTTCATATATTGTGATAAGTTGTGGGACTGTTACATGGAAATTGGATTGCAGTGTTACTCAAATTGAATGGATATTTTTCTGGTATTCATTTTAACTGTGATGTGTGTTGAATGAAAACAGTTGCTTCCATCTAGAGGCAAGATAGCATACCTAGTGATTGAAATTTAGCCTGTTCACCACC
TAACGATTAGTTAGAGGCAATTCCTTCACTCTGTAGGTTTGAAGATCTTAAGGACCAATTAAGGAAAAGGATGTACAAGAAGCGTACTGTTCGAAAGATTACTTTAGTATTAGCTGATGGGATGTCTATTGGGTTGCATACATATGCTTTAATTCGGCCTGCAATACCAGGATCAATCACTTGGCTTGATTCTGTGACCA
ACATTCCCTTGAAGTCAGAAAGCTCTTATATTTGTGCAGACACTGGTGCCTTGCTCACAGAGCCATTACCACGGTTCCATATGTATAAGAATGAAAAGGTTAAACTCACTATTGATGAACTATCTGAAATAAAAAAGGTCACTAGAGTGCCTTTGCGACTTTTAGGATTTAAGCCATTACATTGTTTGAAAGATTACCAT
AATTTGCGGCCAGCAACTTTTCTCTACCCAAGTGATGAGGAGATTAGTGGAAGTACATGTGCATTTATTGCTCTCTATAGGGCAATGTTGCGTTTGCAAAAATATGCTGTGGCATTTTATGGAAAAACATCAATTCCGGAACTGGTTGCTCTAGTTGCACGGGTTGGCTTTTCCTTTCACGTTGTAGTAATTTTTTGGCA
CGATATATAAGAGTGCATTATTTAAGTTGATCTAAAGTTCTTCAAAGGAAAACAGATTATTAATGTTTCTTCAAGTGACTAGATAGAGGTGTTACAAAAAACAAGACAAACCTTACATGGATAGTAAACCAAGATGAACATTTTGATAACTAAATGTCAAATTTGGTTAGAGAGAACATTCATTGAAATCTCCTATCACC
AATAATATAGATCATATTTCTAGGCTAATGACATGGGAAAGAGCATTTGGGAAATGGTATAGTATGATCAAAGTTAGTTCAACATGAC

>TRINITY_DN108810_c0_g1_i10 from sample A4 / Example unspliced isoform:

GTTCATGAGAAAAGTATCCTTCATATATTGTGATAAGTTGTGGGACTGTTACATGGAAATTGGATTGCAGTGTTACTCAAATTGAATGGATATTTTTCTGGTATTCATTTTAACTGTGATGTGTGTTGAATGAAAACAGTTGCTTCCATCTAGAGGCAAGATAGCATACCTAGTGATTGAAATTTAGCCTGTTCACCACC
TAACGATTAGTTAGAGGCAATTCCTTCACTCTGTAGGTTTGAAGATCTTAAGGACCAATTAAGGAAAAGGATGTACAAGAAGCGTACTGTTCGAAAGATTACTTTAGTATTAGCTGATGGGATGTCTATTGGGTTGCATACATATGCTTTAATTCGGCCTGCAATACCAGgtatagtagattctacttttctgttgcatg
gttgaaaattatccgtgagggtgagttacaaagaattttacatgttaatgcaaactatctctctgctgtttttaattatgaatcctccttctgcatcttcagGATCAATCACTTGGCTTGATTCTGTGACCAACATTCCCTTGAAGTCAGAAAGCTCTTATATTTGTGCAGACACTGGTGCCTTGCTCACAGAGCCATTA
CCACGGTTCCATATGTATAAGAATGAAAAGGTTAAACTCACTATTGATGAACTATCTGAAATAAAAAAGGTCACTAGAGTGCCTTTGCGACTTTTAGGATTTAAGCCATTACATTGTTTGAAAGATTACCATAATTTGCGGCCAGCAACTTTTCTCTACCCAAGTGATGAGGAGATTAGTGGAAGTACATGTGCATTTAT
TGCTCTCTATAGGGCAATGTTGCGTTTGCAAAAATATGCTGTGGCATTTTATGGAAAAACATCAATTCCGGAACTGGTTGCTCTAGTTGCACGGGAAGAAATAACAAGTTCAAGTGGCCAAATTGAACCTCCTGGGATGCATATGATCTATCTGCCTTATTCAGATGATATTCGTCATGTTGAAAATCTTCATATGAGTG
CAAATGGTCCAGCACCTCGAGCATCAGAGGAACAAATAGATAGGGCTATATCTATGATGAGAAAGTTAGAATTAAAGGACTTTTCTGTTTATCAAATCTCAAACCCTGCTTTGCAGAGGCATTATGCAATATTGCAAGTTTTAGCCCTTGAAGAAGATGATTTGCCAGAGTCAAAAGATGAGACCATGCCAGATGAAGAG
GGCATGCAAAGACCAGGAATCACAAAAGCTGTACAGGCATTTAAAGATGCTGTATATGGTGAAAATCATGACTTTGAGGAAGCTGAGGCAGAGGCAACTAAAGCCAAAGGCAGTGAAGCATCTCAAAAGAGAAAAGCATCAGCTGAAGTAGCAGCAAAGGAGGCAGCGAGTTATAATTGGAGTGAACTTGCAGACACGGG
AAAG

Intron 3 GTAGTCAAAT ... ATTGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.09	29	4	15
A2	29.03	22	0	9
A3	19.05	34	2	8
A4	45.45	18	3	15
A5	85.71	1	0	6
A6	33.33	14	0	7
A7	26.67	33	0	12
A8	32	17	1	8
A9	32.5	27	0	13
A10	23.33	23	0	7

>TRINITY_DN124441_c3_g1_i12 from sample A1 / Example spliced isoform

GCGCCCTTTTATACACCTCCAGAATCCTCTTAGTCCCGTTTTACCTTTCAAGCTGACGATATTGCGCAGAAGGAAGTGATACCGCCGTGGTACAGACGGGCTCGTTTCTAGATTTTGTTCAGTTGGATGATCAAGCTTAACCGATGGATTGGGATTCAAATGACTTGTTCCAAGATGATGAGGAAGATCAGGATAATACC
TATACACAGGACCGAGAAGCCAACAAGGAGTTTGTTATTTACATGATAGATGCTGGGCCTGATATGTTTTTCCAAATAAAACAAGAGGATGAAGGGAAGACAGAAACACATTTCAGTACTGTGGTCAAGTGTATTGTTGAATCTCTGAAGATTCGCATCATCAACAAAGACTCTGATGAAGTTGCAATATGTTTCTATAA
CACTAGAGAGAAGAAAAACATGCAGGAATCTGAGGGTGTCTATGTTTTCAATGTTTCTGAAAGAGAAGAACTTGACCGTCCAACAGCACGATTGATCAAAGACTTCAGCCACATTGAAGATAAGTTCAAAAGGGAAATTGGGAGTCAAAGCGGAATTATTCCTGGATCAAGAGAAAATCCATTATACAATGCTCTTTGGG
TTGCACAGGGACTCTTACGCAAGGGGTCCACTCGCAGTGCTGACAAGCGTATACTATTATTTACCAATAATGATGATCCTTTTGGAAATGTCGATGGTGGTGCCAAAGCAGACATGAGGAGGACAACAATTCAGCGAGCCAAAGATGCACAAGATTTAGGTATCTCAATTGAGCTTTTCCCCTTGAATAGGCCAGGCGAA
GAATTCAATGTTTCCATTTTCTATTCTGATATGCTTGAGTTGGAGAAGGAGGAAATATCTCAGTTCATGCCGATGGCAGTTGCAAAGTTTGAAGATCTTAAGGACCAATTAAGGAAAAGGATGTACAAGAAGCGTACCGTTCGAAAGATTACTTTAGTATTAGCTGATGGGATGTCTATTGGGTTGCATACATATGCTTT
AATTCGGCCTGCAATACCAGGATCAATCACTTGGCTTGATTCTGTGACCAACATTCCCTTGAAGTCAGAAAGCTCTTATATTTGTGCAGACACTGGTGCCTTGCTCACAGAGCCATTACCACGGTTCCATATGTATAAGAATGAAAAGGTTAAACTCACTATTGATGAACTATCTGAAATAAAAAAGGTCACTAGAGTGC
CTTTGCGACTTTTAGGATTTAAGCCATTACATTGTTTGAAAGATTACCATAATTTGCGGCCAGCAACTTTTCTCTACCCAAGTGATGAGGTATTATCAGCTTGTTTGTATTGCAATTTTCCAATTAGAAAAAAGTGAATGTTATTTGGGAAATTTTCAGTTTGGTTGCTATGTACTCAATGCTAGCCCTCT

>TRINITY_DN124441_c3_g1_i1 from sample A1 / Example unspliced isoform:

CTCTTACGCAAGGGgtagtcaaatggagatggtttttccttacaaggattatacaattttcattcgtggttttcatattttgttttttggtatttctcatcgaatctgaaacttggccagatagttgttgtatctatgttcactgtgtttattgtttcagGTCCACTCGCAGTGCTGACAAGCGTATACTATTATTTACC
AATAATGATGATCCTTTTGGAAATGTCGATGGTGGTGCCAAAGCAGACATGAGGAGGACAACAATTCAGCGAGCCAAAGATGCACAAGATTTAGGTATCTCAATTGAGCTTTTCCCCTTGAATAGGCCAGGCGAAGAATTCAATGTTTCCATTTTCTATTCTGATATGCTTGAGTTGGAGAAGGAGGAAATATCTCAGTT
CATGCCGATGGCAGTTGCAAAGTATGTATCTTCTATTCTAATTCATATTGAAAGCATAAACTCTTGTTACAAAACTGTCGAACCAGCAAAGTGAATTCACTTTGCCTTCTTATAATGGGCACAGGCTATTTCTTA