NA ----> NA


Intron 1 GTTTGCTGTT ... GTCCATGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A124.4125743.183
A216.3320546.740
A314.432774.155
A421.117243.346
A511.0211323.314
A625.53358.612
A719.2118540.644
A825.76496.917
A923.8913730.743
A1014.2321147.435



>TRINITY_DN117915_c1_g2_i2 from sample A3 / Example spliced isoform
ATCAAATGCTATGAGTCTAAAAGTTTCTACACCCAATCGTTCCCCAGCCTATATAATTGCTCTAGAACAGACCCAATCTTTCTCCAGCCTAGATAATTGTTCTAGAACAGAACAAACCTCAGATATATGGAAACATGAATTCTATGTCAGTTCTTCTTGTTAGCAATAGTGTCAAGCATGTGATGTTGAAATGCTGGTAA
CTTTGGAATGGCAGAGTCAATGGAGGCTGGCAGAGCAAATGAGAAGCATTTGGTATTACATGATGGTCTTAACTGTGGGGCCGACAAACAGAACAATGGTACTAGTTTGGCCATGGAGGGGGAACAACCACAACTATGTATAGAAGCAGTTCAGCCCAAAAGGAAAAGAAGGGCAGGCCAATGCAACTTGAGGAAAAGTC
TAGCATGGGACAAAGCCTTCTTCACTAGTGAAGGGGTTCTCAATCCTGAAGAACTATCACTTGTGAACAAGACTTTCAAGAAATCAAATGAGACTGTGTTACCTATTATTGAAGAGGAAGACTCCGTGAGAAGTTCTTTTGGTTCAAACGGCAGCCTTGGGGAAGAAGATCCGGCTTTACAATGTCTCGAAGAAAAACTA
TTTACAGAGCTCCAAACTTCAAAGCCTAAACTTGATAATGAAGTTGATAAGATATGCAATGCTGCATCTTGTGAAACTTTTCCTGCTCAACCATTAGGACTTCCACCATCCAATGTAAAACATTTATCTACAGAAAAAAGGCACATTGCCATGAAAGTTCTTGAGACTCCTTCACAGAAAAAGGCTGCCCTTGGACCCCA
AGCTCCCTTCACTTCAAAAAGGTCAAGTGCTAAAGCCACCGAGGAATTGGAAAAGGCAAGCCAGTCAAATGATGCATATGGAAAGAATTGCTCTGCTGCACCTCCAGTAGTTACAAAAAATTTGTCTAAAGGTCATAAACGCATGAAATATACACATGGAAATGCTGTGCCAGCTAGCTTGACCAGGGCTTCCCATCACA
GAAACTCGCCAGCAAAGATAAAAGAAAATTTTGGCATCACTAGTAGATCTGCTCCATCGCCAAATTCTTCTGCATCTGACAATTTGAATTCTGAAAAACGTCCAGCTTCTTCTTCTTGTAGCCTTGAAAAGTTAAGGAAGTCTCTATTTGAAATGACAAAGGAAGGTACACCTGGGACTAAAGACTCAGACAGAAATGGG
AATTTTCTTGGCACATCTTCTGTGCAATTTTGTCATGGGATGCCTGCATTTCATTCTTCAATGGTTTCTGCTACCTCGACAGCCAGTGGTTCATTATTTCTGACCCACACATCTCGTGAGCAGTTTGCGCCCACCACAGGGGCACGTACATCTGCAGATTCAACAGTTTCTCAATTTGGCAAGAACCATGAATTTTGGAA
CACAATGGCAGGGCACTTGCCTACAAAAGATGATTCTATAAGCATTGCTGGTAATAGTGTGATTTTTAGTGCCAAGAATCCTCCAAGTAATTCTGGCCATGCTTTGCCTGTAACTTTTACTGCAAAGCCCTCAGCCTCAAGAATTCCTAGAAAAGATGATCATATAAGCACTGCTTCTAATAGCATGGTTTTTAGTGCCA
AGGATCCTCCAAGTAATTCTAGTAATGCTTTGCCTGTCACTTTTGCTGCAAAGCCCTCGGGCTTGAGAATGCCTTCACCAAAACTAGGATTTTTTGATTCGACAAAAGTGATCCGTGCACCATATTCCTCTTCCTTACACAGAAGATCTGCCGGCAATCGTGTCCTTCCACCCAGGGCTATGCCCAGAGGGCCAAGCAGT
ATTCATCAGGGTAATAGATTGAAACCCCCAGGTGTTCCTAGTGCAATACCATCCTCCATTTCTGTGAGCAATTTTAGTTTTCCAAACTATAATGCTATTGTACCACCCAGCACTCCAGTGACTTCCAATTTGTTGTCTGATGCTGCCCTGAAGAGTGCTATCACATCCAATCCAGCTCCTCCATTTCAGCCACAGGTTAA
ACCAGACTCTGAAGTTTCTGGTTTGGTCTTGTTGGCACAGAAAAAATTTGGTGTTTCTAAGGTTCAGCAAACTTCATCTGTCCAAAGAAGTGATCAGAAAAAGGTCCCTTCAGATGTTTGTACAGATGCCTCATCAACAGTGGAGATTGGTAAAGCTTCGAATGCGAGTAGTACTAACATTCCTCATCCTGGTCATGGAC
GTGGAATGGCTGTTCTTTCTGATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGAT
AAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGCTCGTGTCTTGGCCTGTGAATTTAGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTGCAAGAGAATGAAATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAA
GGAAATAGAGAAGGTATCTATTGGGTTAGACATTGTTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCAGGATGGTGCTTCCTGTCAAGTTTCTCAG


>TRINITY_DN117915_c1_g2_i7 from sample A3 / Example unspliced isoform:
TACACATGGAAATGCTGTGCCAGgtttgctgttaccagcatatcattcagtttggtatttcctgttagggatctagaatatgcaatatcattccagggggtaccatggcatttattctagggatgtatgcatatgtattaaattggttacaaacttacagacaagctgcttacttgcattaggtttctaggttttgtatg
tttcaaattggaattttatacataaaaatgccatattggttataactgtgcagtgacaattgtatgcaatgaccatggttgtccatgcagCTAGCTTGACCAGGGCTTCCCATCACAGAAACTCGCCAGCAAAGATAAAAGAAAATTTTGGCATCACTAGTAGATCTGCTCCATCGCCAAATTCTTCTGCATCTGACAAT
TTGAATTCTGAAAAACGTCCAGCTTCTTCTTCTTGTAGCCTTGAAAAGTTAAGGAAGTCTCTATTTGAAATGACAAAGGAAGGTACACCTGGGACTAAAGACTCAGACAGAAATGGGAATTTTCTTGGCACATCTTCTGTGCAATTTTGTCATGGGATGCCTGCATTTCATTCTTCAATGGTTTCTGCTACCTCGACAGC
CAGTGGTTCATTATTTCTGACCCACACATCTCGTGAGCAGTTTGCGCCCACCACAGGGGCACGTACATCTGCAGATTCAACAGTTTCTCAATTTGGCAAGAACCATGAATTTTGGAACACAATGGCAGGGCACTTGCCTACAAAAGATGATTCTATAAGCATTGCTGGTAATAGTGTGATTTTTAGTGCCAAGAATCCTC
CAAGTAATTCTGGCCATGCTTTGCCTGTAACTTTTACTGCAAAGCCCTCAGCCTCAAGAATTCCTAGAAAAGATGATCATATAAGCACTGCTTCTAATAGCATGGTTTTTAGTGCCAAGGATCCTCCAAGTAATTCTAGTAATGCTTTGCCTGTCACTTTTGCTGCAAAGCCCTCGGGCTTGAGAATGCCTTCACCAAAA
CTAGGATTTTTTGATTCGACAAAAGTGATCCGTGCACCATATTCCTCTTCCTTACACAGAAGATCTGCCGGCAATCGTGTCCTTCCACCCAGGGCTATGCCCAGAGGGCCAAGCAGTATTCATCAGGGTAATAGATTGAAACCCCCAGGTGTTCCTAGTGCAATACCATCCTCCATTTCTGTGAGCAATTTTAGTTTTCC
AAACTATAATGCTATTGTACCACCCAGCACTCCAGTGACTTCCAATTTGTTGTCTGATGCTGCCCTGAAGAGTGCTATCACATCCAATCCAGCTCCTCCATTTCAGCCACAGGTTAAACCAGACTCTGAAGTTTCTGGTTTGGTCTTGTTGGCACAGAAAAAATTTGGTGTTTCTAAGGTTCAGCAAACTTCATCTGTCC
AAAGAAGTGATCAGAAAAAGGTCCCTTCAGATGTTTGTACAGATGCCTCATCAACAGTGGAGATTGGTAAAGCTTCGAATGCGAGTAGTACTAACATTCCTCATCCTGGTCATGGACGTGGAATGGCTGTTCTTTCTGATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGC
AGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGCTCGTGTCTTGGCCTGTGAATTT
AGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTGCAAGAGAATGAAATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAAGGAAATAGAGAAGGTATCTATTGGGTTAGACATTGTTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCAGGATGGTG
CTTCCTGTCAAGTTTCTCAG



Intron 2 GTTAGTCCAC ... CTGTTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A116.9323333.547.5
A219.313826.533
A314.291922032
A421.7111916.533
A515.22789.514
A626.672228
A719.27881021
A826.8749718
A920.9811312.530
A1015.821331925



>TRINITY_DN100922_c0_g1_i4 from sample A4 / Example spliced isoform
CATAAACGGCTCAAAGAGTTTCGTTCAATCTTTTTTCTTTCACGTTTGCTTAGCCCTTTTCAGATCTGCTTTTCAAGTTTTGCCAAAAGAAACACCGGGGTTCGAATCTCGTGGGGAGCTTGGAATGAGAGATCCAGTCGATGGATCACCAAACGGATGTGGACGGCCTGAGTCTCATAGACGTATCTGCAGAAGATGAC
AGCCTCTTATCATCGCCCGCTTTAAAGTCGCATAACGATGCCGATCGAACTCTTGATTTCCATTCCTCGCCAGCAACCACATTTCGAGTGCAGCAAAGTGCTCTCGCGTCTACCCACCACCGCATATCTCCGTCAGAGTCAATGGAGGCTGGCAGAGCAAATGAGAAGCATTTGGTATTACATGATGGTCTTAACTGTGG
GGCCGACAAACAGAACAATGGTACTAGTTTGGCCATGGAGGGGGAACAACCACAACTATGTATAGAAGCAGTTCAGCCCAAAAGGAAAAGAAGGGCAGGCCAATGCAACTTGAGGAAAAGTCTAGCATGGGACAAAGCCTTCTTCACTAGTGAAGGGGTTCTCAATCCTGAAGAACTATCACTTGTGAACAAGACTTTCA
AGAAATCAAATGAGACTGTGTTACCTATTATTGAAGAGGAAGACTCCGTGAGAAGTTCTTTTGGTTCAAACGGCAGCCTTGGGGAAGAAGATCCGGCTTTACAATGTCTCGAAGAAAAACTATTTACAGAGCTCCAAACTTCAAAGCCTAAACTTGATAATGAAGTTGATAAGATATGCAATGCTGCATCTTGTGAAACT
TTTCCTGCTCAACCATTAGGACTTCCACCATCCAATGTAAAACATTTATCTACAGAAAAAAGGCACATTGCCATGAAAGTTCTTGAGACTCCTTCACAGAAAAAGGCTGCCCTTGGACCCCAAGCTCCCTTCACTTCAAAAAGGTCAAGTGCTAAAGCCACCGAGGAATTGGAAAAGGCAAGCCAGTCAAATGATGCATA
TGGAAAGAATTGCTCTGCTGCACCTCCAGTAGTTACAAAAAATTTGTCTAAAGGTCATAAACGCATGAAATATACACATGGAAATGCTGTGCCAGGTTTGCTGTTACCAGCATATCATTCAGTTTGGTATTTCCTGTTAGGGATCTAGAATATGCAATATCATTCCAGGGGGTACCATGGCATTTATTCTAGGGATGTAT
GCATATGTATTAAATTGGTTACAAACTTACAGACAAGCTGCTTACTTGCATTAGGTTTCTAGGTTTTGTATGTTTCAAATTGGAATTTTATACATAAAAATGCCATATTGGTTATAACTGTGCAGTGACAATTGTATGCAATGACCATGGTTGTCCATGCAGCTAGCTTGACCAGGGCTTCCCATCACAGAAACTCGCCA
GCAAAGATAAAAGAAAATTTTGGCATCACTAGTAGATCTGCTCCATCGCCAAATTCTTCTGCATCTGACAATTTGAATTCTGAAAAACGTCCAGCTTCTTCTTCTTGTAGCCTTGAAAAGTTAAGGAAGTCTCTATTTGAAATGCCAAAGGAAGGTACACCTGGGACTAAAGACTCAGACAGAAATGGGAATTTTCTTGG
CACATCTTCTGTGCAATTTTGTCATGGGATGCCTGCATTTCATTCTTCAATGGTTTCTGCTACCTCGACAGCCAGTGGTTCATTATTTCTGACCCACACATCTCGTGAGCAGTTTGCGCCCACCACAGGGGCACGTACATCTGCAGATTCAACAGTTTCTCAATTTGGCAAGAACCATGAATTTTGGAACACAATGGCAG
GGCACTTGCCTACAAAAGATGATTCTATAAGCATTGCTGGTAATAGTGTGATTTTTAGTGCCAAGAATCCTCCAAGTAATTCTGGCCATGCTTTGCCTGTAACTTTTACTGCAAAGCCCTCAGCCTCAAGAATTCCTAGAAAAGATGATCATATAAGCACTGCTTCTAATAGCATGGTTTTTAGTGCCAAGGATCCTCCA
AGTAATTCTAGTAATGCTTTGCCTGTCACTTTTGCTGCAAAGCCCTCGGGCTTGAGAATGCCTTCACCAAAACTAGGATTTTTTGATTCGACAAAAGTGATCCGTGCACCATATTCCTCTTCCTTACACAGAAGATCTGCCGGCAATCGTGTCCTTCCACCCAGGGCTATGCCCAGAGGGCCAAGCAGTATTCATCAGGG
TAATAGATTGAAACCCCCAGGTGTTCCTAGTGCAATACCATCCTCCATTTCTGTGAGCAATTTTAGTTTTCCAAACTATAATGCTATTGTACCACCCAGCACTCCAGTGACTTCCAATTTGTTGTCTGATGCTGCCCTGAAGAGTGCTATCACATCCAATCCAGCTCCTCCATTTCAGCCACAGGTTAAACCAGACTCTG
AAGTTTCTGGTTTGGTCTTGTTGGCACAGAAAAAATTTGGTGTTTCTAAGGTTCAGCAAACTTCATCTGTCCAAAGAAGTGATCAGAAAAAGGTCCCTTCAGATGTTTGTACAGATGCCTCATCAACAGTGGAGATTGGTAAAGCTTCGAATGCGAGTAGTACTAACATTCCTCATCCTGGTCATGGACGTGGAATGGCT
GTTCTTTCTGATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGATAAGGTTGAGAA
TGGAATCTCTGATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGCTTGTGTCTTGGCCTGTGAATTTAGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTGCAAGAGAATGAAATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAAGGAAATAGAGA
AGGTATCTATTGGGTTAGACATTGTTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCAGGATGGTGCTTCCTGTCAAGTTTCTCAGGCAAGTATCTCTATGCAAGATGAGGATTTACATGATAATGTTAGAAATGGAAATTCTAATTTGGTTTGTCAGTTGCAGTTGGAAAATGAAATGGAGACTACATCTCAG
CCTGTAAAATTTGAGCAGTCCCTGGCTACAAGAAAAGGAGAAACACAGAAGGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGATAAGGGTTTGGATGTAGTTCAAAATGAAGTAAATGATAGAGCTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAATC
CATATTATCTGATGTTACTGATAGCTTGGAATTCAAAACTGGAGTGGTGTTTAATCTTAAAGAGAATGTCACAAATTCTTCCAACAATTATCAAAATAATACCACCGATACGCTTATTACTCCATCCCTATTGTCAGGCTGTCCTCCATGTGAAGCTTCTCCTTCAGTCATAGAACATAAATCTATAGATAAAGATGATT
CTTCATTACGTAATATGAAATTGTCTGATAGCTCGGTTACGCTAAAAGTGCAAATGATGTCTGGGCTCAAAGTTGCTGGTAATGAAGTTAAATTTAAGGGAAGAAGTCCTTTGGCTACCAATTACGAGCTGCTGAACTCTACTGCTAAGGAAATATCCCAGAAACATGTCAAAGAACAGAGGTGTTCAATGGATGGTTCA
GAGAGATTGACATCCTTGCCTAGGTCATGTCCTGTATTGGATGATACAGCAGAGAAAGAGAACAGCAGGCCAGAAGGGCAAGATGGAGTTGGCATGTCAGAAGTGCATGGTGCAGATGGTCATTATGTAAGCTCTGGAAGAAAGTCAATGGGTAACCTAAGTGTTCAAGTACAGAAAATAAAGAACCCTTTAGATGCTTC
GCCATTTTCAGAAGAGTGGATTGCTGCTATACAAGCTGTGGGTGAGGAATGGCTTCAAATGAAAAGAGGACCAGTGCAGAACTCACCACCAAACAAAATGGTTCCCCAACCAGGTCCATGGTCTCCGGTTCGGCGCCCTAACCAGCAACTAGGTCCATTTGATTGTACTAAATATACAAATGCAGCGGACAGTACTGATG
TTTATGAAGCTGAATAAGATGCCTGTGGTAGGCATGCTTTTCATGTATGGCAGAAGGGTGATCAGTCTTGTACATAGTTCCTAATTACCAATATGTACGAACTATGAAGTTACCATCTGCGGCAAACAAAGGCATGGAATCTGGCTTGCAGGGAACTCTTATTATATGCTATGATGCCCTTATGCCAGGGTCTGTTCCAG
TAGAATCTCCCATGTTTCTTGTTTTGAAAGATTGTGGTCAAGAACGATACAATAGCAGGCTAAAAGTCTGTTGGTGGAGCAGGATCTGATAGTTTGTTGCAAGTGACCTTAGTGTTGATCATTTTAGATATTTTGTGATTTATTCTGTTTACAAACTTGTCTTGTAAGTGAACCCATCTCAGGGCTATTGTAATCTGTTT
TCATTAGGCTTTCCTTTGTTGGCTGAGAAAATTTCTCAGAAGGATCAATTTTTTGAGCATTTCAGAAATATTTCTGCATTCCAGGCTCCTAAGACAATCCAAATTACACAATGCATTGAACGTTGCAGTTGTGTATGGGTACTCTTATTTACTTCTGTACATGAGTGCTTGATGGTGCAGAAGAAATGATGGAATTCTTT
TCATAAGTAGCGGTTTGGTGCACCAGCTACAGGAAATATATCTGGATGTTAAGAAGGTACTTGCAGCAATTGTTCATGATGTCTTTGTAATTTGGATGTATAAAACTGTAGATAATAAGCTTCCTTTAACACATCTAATTTAAAAAAGCTGTTAAGTGTAGGTCTCTGTG


>TRINITY_DN100922_c0_g1_i2 from sample A4 / Example unspliced isoform:
CCTTCACCCAAACTAGGATTTTTTGATTCGgttagtccacattctctttctcagatctcatgcattgctacattttgtcgtgtgacaaccgtgaagggacatttgactgcaaatgtaatatcaccagactaattgcgttttggctgtttgcagACAAAAGTGATCCGTGCACCATATTCCTCTTCCTTACACAGAAGATC
TGCCGGCAATCGTGTCCTTCCACCCAGGGCTATGCCCAGAGGGCCAAGCAGTATTCATCAGGGTAATAGATTGAAACCCCCAGGTGTTCCTAGTGCAATACCATCCTCCATTTCTGTGAGCAATTTTAGTTTTCCAAACTATAATGCTATTGTACCACCCAGCACTCCAGTGACTTCCAATTTGTTGTCTGATGCTGCCC
TGAAGAGTGCTATCACATCCAATCCAGCTCCTCCATTTCAGCCACAGGTTAAACCAGACTCTGAAGTTTCTGGTTTGGTCTTGTTGGCACAGAAAAAATTTGGTGTTTCTAAGGTTCAGCAAACTTCATCTGTCCAAAGAAGTGATCAGAAAAAGGTCCCTTCAGATGTTTGTACAGATGCCTCATCAACAGTGGAGATT
GGTAAAGCTTCGAATGCGAGTAGTACTAACATTCCTCATCCTGGTCATGGACGTGGAATGGCTGTTCTTTCTGATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGA
AGAACAATATGGACTTTCAAATAGTAATTCAGAAGGAACCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGCTTGTGTCTTGGCCTGTGAATTTAGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTGCAAGAGAATGAAATGG
AGCCTGAGTCTTGGCGTGTAAGATCAGGACATTTTCCATCCCTAGGTCATGGACGTGAAATGGCTGTTCTTTCTGATGCCA



Intron 3 GCAAGTATCT ... TCAGTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A160.3238399324
A259.38194290277.3
A355.84227302272
A461.48199.6334.6302.6
A562.8363112101
A66481139149
A765.96201365414
A866.12143254304.3
A967.02167342336.6
A1064.29170308304



>TRINITY_DN119871_c0_g1_i2 from sample A1 / Example spliced isoform
TTTCCCGCTCGTGGAGAGCTTTGAATTTCTTCTACCATAAACGGCTCAAAGAGTTTCGTTCAATCTTTTTTCTTTCACGTTTGCTTAGCCCTTTTCAGATCTGCTTTTCAAGTTTTGCCAAAAGAAACACCGGGGTTCGAATCTCGTGGGGAGCTTGGAATGAGAGATCCAGTCGATGGATCACCAAACGGATGTGGACG
GCCTGAGTCTCATAGACGTATCTGCAGAAGATGACAGCCTCTTATCATCGCCCGCTTTAAAGTCGCATAACGATGCCGATCGAACTCTTGATTTCCATTCCTCGCCAGCAACCACATTTCGAGTGCAGCAAAGTGCTCTCGCGTCTACCCACCACCGCATATCTCCGTCAGAGTCAATGGAGGCTGGCAGAGCAAATGAG
AAGCATTTGGTATTACATGATGGTCTTAACTGTGGGGCCGACAAACAGAACAATGGTACTAGTTTGGCCATGGAGGGGGAACAACCACAACTATGTATAGAAGCAGTTCAGCCCAAAAGGAAAAGAAGGGCAGGCCAATGCAACTTGAGGAAAAGTCTAGCATGGGACAAAGCCTTCTTCACTAGTGAAGGGGTTCTCAA
TCCTGAAGAACTATCACTTGTGAACAAGACTTTCAAGAAATCAAATGAGACTGTGTTACCTATTATTGAAGAGGAAGACTCCGTGAGAAGTTCTTTTGGTTCAAACGGCAGCCTTGGGGAAGAAGATCCGGCTTTACAATGTCTCGAAGAAAAACTATTTACAGAGCTCCAAACTTCAAAGCCTAAACTTGATAATGAAG
TTGATAAGATATGCAATGCTGCATCTTGTGAAACTTTTCCTGCTCAACCATTAGGACTTCCACCATCCAATGTAAAACATTTATCTACAGAAAAAAGGCACATTGCCATGAAAGTTCTTGAGACTCCTTCACAGAAAAAGGCTGCCCTTGGACCCCAAGCTCCCTTCACTTCAAAAAGGTCAAGTGCTAAAGCCACCGAG
GAATTGGAAAAGGCAAGCCAGTCAAATGATGCATATGGAAAGAATTGCTCTGCTGCACCTCCAGTAGTTACAAAAAATTTGTCTAAAGGTCATAAACGCATGAAATATACACATGGAAATGCTGTGCCAGCTAACTTGACCAGGGCTTCCCATCACAGAAACTCGCCAGCAAAGATAAAAGAAAATTTTGGCATCACTAG
TAGATCTGCTCCATCGCCAAATTCTTCTGCATCTGACAATTTGAATTCTGAAAAACGTCCAGCTTCTTCTTCTTGTAGCCTTGAAAAGTTAAGGAAGTCTCTATTTGAAATGACAAAGGAAGGTACACCTGGGACTAAAGACTCAGACAGAAATGGGAATTTTCTTGGCACATCTTCTGTGCAATTTTGTCATGGGATGC
CTGCATTTCATTCTTCAATGGTTTCTGCTACCTCGACAGCCAGTGGTTCATTATTTCTGACCCACACATCTCGTGAGCAGTTTGCGCCCACCACAGGGGCACGTACATCTGCAGATTCAACAGTTTCTCAATTTGGCAAGAACCATGAATTTTGGAACACAATGGCAGGGCACTTGCCTACAAAAGATGATTCTATAAGC
ATTGCTGGTAATAGTGTGATTTTTAGTGCCAAGAATCCTCCAAGTAATTCTGGCCATGCTTTGCCTGTAACTTTTACTGCAAAGCCCTCAGCCTCAAGAATTCCTAGAAAAGATGATCATATAAGCACTGCTTCTAATAGCATGGTTTTTAGTGCCAAGGATCCTCCAAGTAATTCTAGTAATGCTTTGCCTGTCACTTT
TGCTGCAAAGCCCTCGGGCTTGAGAATGCCTTCACCAAAACTAGGATTTTTTGATTCGACAAAAGTGATCCGTGCACCATATTCCTCTTCCTTACACAGAAGATCTGCCGGCAATCGTGTCCTTCCACCCAGGGCTATGCCCAGAGGGCCAAGCAGTATTCATCAGGGTAATAGATTGAAACCCCCAGGTGTTCCTAGTG
CAATACCATCCTCCATTTCTGTGAGCAATTTTAGTTTTCCAAACTATAATGCTATTGTACCACCCAGCACTCCAGTGACTTCCAATTTGTTGTCTGATGCTGCCCTGAAGAGTGCTATCACATCCAATCCAGCTCCTCCATTTCAGCCACAGGTTAAACCAGACTCTGAAGTTTCTGGTTTGGTCTTGTTGGCACAGAAA
AAATTTGGTGTTTCTAAGGTTCAGCAAACTTCATCTGTCCAAAGAAGTGATCAGAAAAAGGTCCCTTCAGATGTTTGTACAGATGCCTCATCAACAGTGGAGATTGGTAAAGCTTCGAATGCGAGTAGTACTAACATTCCTCATCCTGGTCATGGACGTGGAATGGCTGTTCTTTCTGATGCCAGGACAAATGATCTGAC
CAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAACCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCAGC
AGGAGAACAAAATGAAGCTTGTGTCTTGGCCTGTGAATTTAGAACATCTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTCTGCAAGAGAATGAAATGGAGCCTGAGTCTTGGCGTGTAAGATCAGGACATTTTCCATCCCTAGGTCATGGACGTGAAATGGCTGTTCTTTCTGATGCCAGGACAAATGATCTG
ACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCA
GCAGGAGAACAAAATGAAGCTCGTGTCTTGGCCTGTGAATTTAGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTGCAAGAGAATGAAATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAAGGAAATAGAGAAGGTATCTATTGGGTTAGACATTGTTTCAA
CCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCAGGATGGTGCTTCCTGTCAAGTTTCTCAGTTGGAAAATGAAATGGAGACTACATCTCAGCCTGTAAAATTTGAGCAGTCCCTGGCTACAAGAAAAGGAGAAACACAGAAGGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGATAAGGGTTTGGATGTA
GTTCAAAATGAAGTAAATGATAGAGCTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAATCCATATTATCTGATGTTACTGATAGCTTGGAATTCAAAACTGGAGTGGTGTTTAATCTTAAAGAGAATGTCACAAATTCTTCCAACAATTATCAAAATAATACCACCGA
TACGCTTATTACTCCATCCCTATTGTCAGGCTGTCCTCCATGTGAAGCTTTTCCTTCAGTCATAGAACATAAATCTATAGATAAAGATGATTCTTCATTACGTAATATGAAATTGTCTGATAGCTCGGTTACGCTAAAAGTGCAAATGATGTCTGGGCTCAAAGTTGCTGGTAATGAAGTTAAATTTAAGGGAAGAAGTC
CTTTGGCTACCAATTACGAGCTGCTGAACTCTACTGCTAAGGAAATATCCCAGAAACATGTCAAAGAACAGAGGTGTTCAATGGATGGTTCAGAGAGATTGACATCCTTGCCTAGGTCATGTCCTGTATTGGATGATACAGCAGAGAAAGAGAACAGCAGGCCAGAAGGGCAAGATGGAGTTGGCATGTCAGAAGTGCAT
GGTGCAGATGGTCATTATGTAAGCTCTGGAAGAAAGTCAATGGGTAACCTAAGTGTTCAAGTACAGAAAATAAAGAACCCTTTAGATGCTTCGCCATTTTCAGAAGAGTGGATTGCTGCTATACAAGCTGTGGGTGAGGAATGGCTTCAAATGAAAAGAGGACCAGTGCAGAACTCACCACCAAACAAAATGGTTCCCCA
ACCAGGTCCATGGTCTCCGGTTCGGCGCCCTAACCAGCAACTAGGTCCATTTGATTGTACTAAATATACAAATGCAGCGGACAGTACTGATGTTTATGAAGCTGAATAAGATGCCTGTGGTAGGCATGCTTTTCATGTATGGCAGAAGGGTGATCAGTCTTGTACATAGTTCCTAATTACCAATATGTACGAACTATGAA
GTTACCATCTGCGGCAAACAAAGGCATGGAATCTGGCTTGCAGGGAACTCTTATTATATGCTATGATGCCCTTATGCCAGGGTCTGTTCCAGTAGAATCTCCCATGTTTCTTGTTTTGAAAGATTGTGGTCAAGAACGATACAATAGCAGGCTAAAAGTCTGTTGGTGGAGCAGGATCTGATAGTTTGTTGCAAGTGACC
TTAGTGTTGATCATTTTAGATATTTTGTGATTTATTCTGTTTACAAACTTGTCTTGTAAGTGAACCCATCTCAGGGCTATTGTAATCTGTTTTCATTAGGCTTTCCTTTGTTGGCTGAGAAAATTTCTCAGAAGGATCAATTTTTTGAGCATTTCAGAAATATTTCTGCATTCCAGGCTCCTAAGACAATCCAAATTACA
CAATGCATTGAACGTTGCAGTTGTGTATGGGTACTCTTATTTACTTCTGTACATGAGTGCTTGATGGTGCAGAAGAAATGATGGAATTCTTTTCATAAGTAGCGGTTTGGTGCACCAGCTACAGGAAATATATCTGGATGTTAAGAAGGTACTTGCAGCAATTGTTCATGATGTCTTTGTAATTTGGATGTATAAAACTG
TAGATAATAAGCTTCC


>TRINITY_DN119871_c0_g1_i9 from sample A1 / Example unspliced isoform:
TTTTACTTTAAGAATTTAATATTACCATTTAGTATTACCAAATACTTCTGCATAACTCAACATAGTGTATTTTTTTCAGTCAATTCAGCCGGTTCAGGTTGTGATTCGGTTGAGTTAAACGGTTTGGTACCAAACTGGACTCCAACTCGGCTGTTTCACTACCGAACCGGCCACCTGATCCGGTTTGAAGGCCGAGTCAT
CTCAGTCAACTCGGCCAAGTCGGAGTCCAAGTTGACCGCTTTTTAAAACAGAGTCTTAATCAAAGCAGAGTCAATGGAGGCTGGCAGAGCAAATGAGAAGCATTTGGTATTACATGATGGTCTTAACTGTGGGGCCGACAAACAGAACAATGGTACTAGTTTGGCCATGGAGGGGGAACAACCACAACTATGTATAGAAG
CAGTTCAGCCCAAAAGGAAAAGAAGGGCAGGCCAATGCAACTTGAGGAAAAGTCTAGCATGGGACAAAGCCTTCTTCACTAGTGAAGGGGTTCTCAATCCTGAAGAACTATCACTTGTGAACAAGACTTTCAAGAAATCAAATGAGACTGTGTTACCTATTATTGAAGAGGAAGACTCCGTGAGAAGTTCTTTTGGTTCA
AACGGCAGCCTTGGGGAAGAAGATCCGGCTTTACAATGTCTCGAAGAAAAACTATTTACAGAGCTCCAAACTTCAAAGCCTAAACTTGATAATGAAGTTGATAAGATATGCAATGCTGCATCTTGTGAAACTTTTCCTGCTCAACCATTAGGACTTCCACCATCCAATGTAAAACATTTATCTACAGAAAAAAGGCACAT
TGCCATGAAAGTTCTTGAGACTCCTTCACAGAAAAAGGCTGCCCTTGGACCCCAAGCTCCCTTCACTTCAAAAAGGTCAAGTGCTAAAGCCACCGAGGAATTGGAAAAGGCAAGCCAGTCAAATGATGCATATGGAAAGAATTGCTCTGCTGCACCTCCAGTAGTTACAAAAAATTTGTCTAAAGGTCATAAACGCATGA
AATATACACATGGAAATGCTGTGCCAGCTAACTTGACCAGGGCTTCCCATCACAGAAACTCGCCAGCAAAGATAAAAGAAAATTTTGGCATCACTAGTAGATCTGCTCCATCGCCAAATTCTTCTGCATCTGACAATTTGAATTCTGAAAAACGTCCAGCTTCTTCTTCTTGTAGCCTTGAAAAGTTAAGGAAGTCTCTA
TTTGAAATGACAAAGGAAGGTACACCTGGGACTAAAGACTCAGACAGAAATGGGAATTTTCTTGGCACATCTTCTGTGCAATTTTGTCATGGGATGCCTGCATTTCATTCTTCAATGGTTTCTGCTACCTCGACAGCCAGTGGTTCATTATTTCTGACCCACACATCTCGTGAGCAGTTTGCGCCCACCACAGGGGCACG
TACATCTGCAGATTCAACAGTTTCTCAATTTGGCAAGAACCATGAATTTTGGAACACAATGGCAGGGCACTTGCCTACAAAAGATGATTCTATAAGCATTGCTGGTAATAGTGTGATTTTTAGTGCCAAGAATCCTCCAAGTAATTCTGGCCATGCTTTGCCTGTAACTTTTACTGCAAAGCCCTCAGCCTCAAGAATTC
CTAGAAAAGATGATCATATAAGCACTGCTTCTAATAGCATGGTTTTTAGTGCCAAGGATCCTCCAAGTAATTCTAGTAATGCTTTGCCTGTCACTTTTGCTGCAAAGCCCTCGGGCTTGAGAATGCCTTCACCAAAACTAGGATTTTTTGATTCGACAAAAGTGATCCGTGCACCATATTCCTCTTCCTTACACAGAAGA
TCTGCCGGCAATCGTGTCCTTCCACCCAGGGCTATGCCCAGAGGGCCAAGCAGTATTCATCAGGGTAATAGATTGAAACCCCCAGGTGTTCCTAGTGCAATACCATCCTCCATTTCTGTGAGCAATTTTAGTTTTCCAAACTATAATGCTATTGTACCACCCAGCACTCCAGTGACTTCCAATTTGTTGTCTGATGCTGC
CCTGAAGAGTGCTATCACATCCAATCCAGCTCCTCCATTTCAGCCACAGGTTAAACCAGACTCTGAAGTTTCTGGTTTGGTCTTGTTGGCACAGAAAAAATTTGGTGTTTCTAAGGTTCAGCAAACTTCATCTGTCCAAAGAAGTGATCAGAAAAAGGTCCCTTCAGATGTTTGTACAGATGCCTCATCAACAGTGGAGA
TTGGTAAAGCTTCGAATGCGAGTAGTACTAACATTCCTCATCCTGGTCATGGACGTGGAATGGCTGTTCTTTCTGATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAAT
GAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAACCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGCTTGTGTCTTGGCCTGTGAATTTAGAACATCTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTCTGCAAGAGAATGAAAT
GGAGCCTGAGTCTTGGCGTGTAAGATCAGGACATTTTCCATCCCTAGGTCATGGACGTGAAATGGCTGTTCTTTCTGATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAA
ATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGCTCGTGTCTTGGCCTGTGAATTTAGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTGCAAGAGAATGAA
ATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAAGGAAATAGAGAAGGTATCTATTGGGTTAGACATTGTTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCAGGATGGTGCTTCCTGTCAAGTTTCTCAGgcaagtatctctatgcaagatgaggatttacatgataatgt
tagaaatggaaattctaatttggtttgtcagttgcagTTGGAAAATGAAATGGAGACTACATCTCAGCCTGTAAAATTTGAGCAGTCCCTGGCTACAAGAAAAGGAGAAACACAGAAGGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGATAAGGGTTTGGATGTAGTTCAAAATGAAGTAAATGATAGAG
CTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAATCCATATTATCTGATGTTACTGATAGCTTGGAATTCAAAACTGGAGTGGTGTTTAATCTTAAAGAGAATGTCACAAATTCTTCCAACAATTATCAAAATAATACCACCGATACGCTTATTACTCCATCCCTATTG
TCAGGCTGTCCTCCATGTGAAGCTTTTCCTTCAGTCATAGAACATAAATCTATAGATAAAGATGATTCTTCATTACGTAATATGAAATTGTCTGATAGCTCGGTTACGCTAAAAGTGCAAATGATGTCTGGGCTCAAAGTTGCTGGTAATGAAGTTAAATTTAAGGGAAGAAGTCCTTTGGCTACCAATTACGAGCTGCT
GAACTCTACTGCTAAGGAAATATCCCAGAAACATGTCAAAGAACAGAGGTGTTCAATGGATGGTTCAGAGAGATTGACATCCTTGCCTAGGTCATGTCCTGTATTGGATGATACAGCAGAGAAAGAGAACAGCAGGCCAGAAGGGCAAGATGGAGTTGGCATGTCAGAAGTGCATGGTGCAGATGGTCATTATGTAAGCT
CTGGAAGAAAGTCAATGGGTAACCTAAGTGTTCAAGTACAGAAAATAAAGAACCCTTTAGATGCTTCGCCATTTTCAGAAGAGTGGATTGCTGCTATACAAGCTGTGGGTGAGGAATGGCTTCAAATGAAAAGAGGACCAGTGCAGAACTCACCACCAAACAAAATGGTTCCCCAACCAGGTCCATGGTCTCCGGTTCGG
CGCCCTAACCAGCAACTAGGTCCATTTGATTGTACTAAATATACAAATGCAGCGGACAGTACTGATGTTTATGAAGCTGAATAAGATGCCTGTGGTAGGCATGCTTTTCATGTATGGCAGAAGGGTGATCAGTCTTGTACATAGTTCCTAATTACCAATATGTACGAACTATGAAGTTACCATCTGCGGCAAACAAAGGC
ATGGAATCTGGCTTGCAGGGAACTCTTATTATATGCTATGATGCCCTTATGCCAGGGTCTGTTCCAGTAGAATCTCCCATGTTTCTTGTTTTGAAAGATTGTGGTCAAGAACGATACAATAGCAGGCTAAAAGTCTGTTGGTGGAGCAGGATCTGATAGTTTGTTGCAAGTGACCTTAGTGTTGATCATTTTAGATATTT
TGTGATTTATTCTGTTTACAAACTTGTCTTGTAAGTGAACCCATCTCAGGGCTATTGTAATCTGTTTTCATTAGGCTTTCCTTTGTTGGCTGAGAAAATTTCTCAGAAGGATCAATTTTTTGAGCATTTCAGAAATATTTCTGCATTCCAGGCTCCTAAGACAATCCAAATTACACAATGCATTGAACGTTGCAGTTGTG
TATGGGTACTCTTATTTACTTCTGTACATGAGTGCTTGATGGTGCAGAAGAAATGATGGAATTCTTTTCATAAGTAGCGGTTTGGTGCACCAGCTACAGGAAATATATCTGGATGTTAAGAAGGTACTTGCAGCAATTGTTCATGATGTCTTTGTAATTTGGATGTATAAAACTGTAGATAATAAGCTTCC



Intron 4 GTCATAAACG ... GCTGTGCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A169.44107.9230255.9
A273.4271.9187205
A380.3279.2313.7326.5
A474.3857157172
A577.334.6117113
A678.2610.13735
A776.7253.4165185
A875.2215.94749
A970.5351.8110137
A1079.854.1213211



>TRINITY_DN118351_c0_g2_i3 from sample A2 / Example spliced isoform
CAAATTTGTGGACTTATATATCTTTCCCGCTCGTGGAGAGCTTTGAATTTCTTCTACCATAAACGGCTCAAAGAGTTTCGTTCAATCTTTTTTCTTTCACGTTTGCTTAGCCCTTTTCAGATCTGCTTTTCAAGTTTTGCCAAAAGAAACACCGGGGTTCGAATCTCGTGGGGAGCTTGGAATGAGAGATCCAGTCGATG
GATCACCAAACGGATGTGGACGGCCTGAGTCTCATAGACGTATCTGCAGAAGATGACAGCCTCTTATCATCGCCCGCTTTAAAGTCGCATAACGATGCCGATCGAACTCTTGATTTCCATTCCTCGCCAGCAACCACATTTCGAGTGCAGCAAAGTGCTCTCGCGTCTACCCACCACCGCATATCTCCGTCAGAGTCAAT
GGAGGCTGGCAGAGCAAATGAGAAGCATTTGGTATTACATGATGGTCTTAACTGTGGGGCCGACAAACAGAACAATGGTACTAGTTTGGCCATGGAGGGGGAACAACCACAACTATGTATAGAAGCAGTTCAGCCCAAAAGGAAAAGAAGGGCAGGCCAATGCAACTTGAGGAAAAGTCTAGCATGGGACAAAGCCTTCT
TCACTAGTGAAGGGGTTCTCAATCCTGAAGAACTATCACTTGTGAACAAGACTTTCAAGAAATCAAATGAGACTGTGTTACCTATTATTGAAGAGGAAGACTCCGTGAGAAGTTCTTTTGGTTCAAACGGCAGCCTTGGGGAAGAAGATCCGGCTTTACAATGTCTCGAAGAAAAACTATTTACAGAGCTCCAAACTTCA
AAGCCTAAACTTGATAATGAAGTTGATAAGATATGCAATGCTGCATCTTGTGAAACTTTTCCTGCTCAACCATTAGGACTTCCACCATCCAATGTAAAACATTTATCTACAGAAAAAAGGCACATTGCCATGAAAGTTCTTGAGACTCCTTCACAGAAAAAGGCTGCCCTTGGACCCCAAGCTCCCTTCACTTCAAAAAG
GTCAAGTGCTAAAGCCACCGAGGAATTGGAAAAGGCAAGCCAGTCAAATGATGCATATGGAAAGAATTGCTCTGCTGCACCTCCAGTAGTTACAAAAAATTTGTCTAAAGCTAACTTGACCAGGGCTTCCCATCACAGAAACTCGCCAGCAAAGATAAAAGAAAATTTTGGCATCACTAGTAGATCTGCTCCATCGCCAA
ATTCTTCTGCATCTGACAATTTGAATTCTGAAAAACGTCCAGCTTCTTCTTCTTGTAGCCTTGAAAAGTTAAGGAAGTCTCTATTTGAAATGACAAAGGAAGGTACACCTGGGACTAAAGACTCAGACAGAAATGGGAATTTTCTTGGCACATCTTCTGTGCAATTTTGTCATGGGATGCCTGCATTTCATTCTTCAATG
GTTTCTGCTACCTCGACAGCCAGTGGTTCATTATTTCTGACCCACACATCTCGTGAGCAGTTTGCGCCCACCACAGGGGCACGTACATCTGCAGATTCAACAGTTTCTCAATTTGGCAAGAACCATGAATTTTGGAACACAATGGCAGGGCACTTGCCTACAAAAGATGATTCTATAAGCATTGCTGGTAATAGTGTGAT
TTTTAGTGCCAAGAATCCTCCAAGTAATTCTGGCCATGCTTTGCCTGTAACTTTTACTGCAAAGCCCTCAGCCTCAAGAATTCCTAGAAAAGATGATCATATAAGCACTGCTTCTAATAGCATGGTTTTTAGTGCCAAGGATCCTCCAAGTAATTCTAGTAATGCTTTGCCTGTCACTTTTGCTGCAAAGCCCTCGGGCT
TGAGAATGCCTTCACCAAAACTAGGATTTTTTGATTCGACAAAAGTGATCCGTGCACCATATTCCTCTTCCTTACACAGAAGATCTGCCGGCAATCGTGTCCTTCCACCCAGGGCTATGCCCAGAGGGCCAAGCAGTATTCATCAGGGTAATAGATTGAAACCCCCAGGTGTTCCTAGTGCAATACCATCCTCCATTTCT
GTGAGCAATTTTAGTTTTCCAAACTATAATGCTATTGTACCACCCAGCACTCCAGTGACTTCCAATTTGTTGTCTGATGCTGCCCTGAAGAGTGCTATCACATCCAATCCAGCTCCTCCATTTCAGCCACAGGTTAAACCAGACTCTGAAGTTTCTGGTTTGGTCTTGTTGGCACAGAAAAAATTTGGTGTTTCTAAGGT
TCAGCAAACTTCATCTGTCCAAAGAAGTGATCAGAAAAAGGTCCCTTCAGATGTTTGTACAGATGCCTCATCAACAGTGGAGATTGGTAAAGCTTCGAATGCGAGTAGTACTAACATTCCTCATCCTGGTCATGGACGTGGAATGGCTGTTCTTTCTGATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGAAC
AATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGCTT
GTGTCTTGGCCTGTGAATTTAGAACATCTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTCTGCAAGAGAATGAAATGGAGCCTGAGTCTTGGCGTGTAAGATCAGGACATTTTCCATCCCTAGGTCATGGACGTGAAATGGCTGTTCTTTCTGATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGA
ACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGC
TTGTGTCTTGGCCTGTGAATTTAGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTGCAAGAGAATGAAATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAAGGAAATAGAGAAGGTATCTATTGGGTTAGACATTGTTTCAACCACTGGTTCCGTTTCAGTT
CAGATTGAAAAGCAGGATGGTGCTTCCTGTCAAGTTTCTCAGGCAAGTATCTCTATGCAAGATGAGGATTTACATGATAATGTTAGAAATGGAAATTCTAATTTGGTTTGTCAGTTGCAGTTGGAAAATGAAATGGAGACTACATCTCAGCCTGTAAAATTTGAGCAGTCCCTGGCTACAAGAAAAGGAGAAACACAGAA
GGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGATAAGGGTTTGGATGTAGTTCAAAATGAAGTAAATGATAGAGCTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAATCCATATTATCTGATGTTACTGATAGCTTGGAATTCAAAACTGGAGTGGTGT
TTAATCTTAAAGAGAATGTCACAAATTCTTCCAACAATTATCAAAATAATACCACCGATACGCTTATTACTCCATCCCTATTGTCAGGCTGTCCTCCATGTGAAGCTTTTCCTTCAGTCATAGAACATAAATCTATAGATAAAGATGATTCTTCATTACGTAATATGAAATTGTCTGATAGCTCGGTTACGCTAAAAGTG
CAAATGATGTCTGGGCTCAAAGTTGCTGGTAATGAAGTTAAATTTAAGGGAAGAAGTCCTTTGGCTACCAATTACGAGCTGCTGAACTCTACTGCTAAGGAAATATCCCAGAAACATGTCAAAGAACAGAGGTGTTCAATGGATGGTTCAGAGAGATTGACATCCTTGCCTAGGTCATGTCCTGTATTGGATGATACAGC
AGAGAAAGAGAACAGCAGGCCAGAAGGGCAAGATGGAGTTGGCATGTCAGAAGTGCATGGTGCAGATGGTCATTATGTAAGCTCTGGAAGAAAGTCAATGGGTAACCTAAGTGTTCAAGTACAGAAAATAAAGAACCCTTTAGATGCTTCGCCATTTTCAGAAGAGTGGATTGCTGCTATACAAGCTGTGGGTGAGGAAT
GGCTTCAAATGAAAAGAGGACCAGTGCAGAACTCACCACCAAACAAAATGGTTCCCCAACCAGGTCCATGGTCTCCGGTTCGGCGCCCTAACCAGCAACTAGGTCCATTTGATTGTACTAAATATACAAATGCAGCGGACAGTACTGATGTTTATGAAGCTGAATAAGATGCCTGTGGTAGGCATGCTTTTCATGTATGG
CAGAAGGGTGATCAGTCTTGTACATAGTTCCTAATTACCAATATGTACGAACTATGAAGTTACCATCTGCGGCAAACAAAGGCATGGAATCTGGCTTGCAGGGAACTCTTATTATATGCTATGATGCCCTTATGCCAGGGTCTGTTCCAGTAGAATCTCCCATGTTTCTTGTTTTGAAAGATTGTGGTCAAGAACGATAC
AATAGCAGGCTAAAAGTCTGTTGGTGGAGCAGGATCTGATAGTTTGTTGCAAGTGACCTTAGTGTTGATCATTTTAGATATTTTGTGATTTATTCTGTTTACAAACTTGTCTTGTAAGTGAACCCATCTCAGGGCTATTGTAATCTGTTTTCATTAGGCTTTCCTTTGTTGGCTGAGAAAATTTCTCAGAAGGATCAATT
TTTTGAGCATTTCAGAAATATTTCTGCATTCCAGGCTCCTAAGACAATCCAAATTACACAATGCATTGAACGTTGCAGTTGTGTATGGGTACTCTTATTTACTTCTGTACATGAGTGCTTGATGGTGCAGAAGAAATGATGGAATTCTTTTCATAAGTAGCGGTTTGGTGCACCAGCTACAGGAAATATATCTGGATGTT
AAGAAGGTACTTGCAGCAATTGTTCATGATGTCTTTGTAATTTGGATGTATAAAACTGTAGATAATAAGCTTCCTTTAACACATCT


>TRINITY_DN118351_c0_g2_i8 from sample A2 / Example unspliced isoform:
CAAATTTGTGGACTTATATATCTTTCCCGCTCGTGGAGAGCTTTGAATTTCTTCTACCATAAACGGCTCAAAGAGTTTCGTTCAATCTTTTTTCTTTCACGTTTGCTTAGCCCTTTTCAGATCTGCTTTTCAAGTTTTGCCAAAAGAAACACCGGGGTTCGAATCTCGTGGGGAGCTTGGAATGAGAGATCCAGTCGATG
GATCACCAAACGGATGTGGACGGCCTGAGTCTCATAGACGTATCTGCAGAAGATGACAGCCTCTTATCATCGCCCGCTTTAAAGTCGCATAACGATGCCGATCGAACTCTTGATTTCCATTCCTCGCCAGCAACCACATTTCGAGTGCAGCAAAGTGCTCTCGCGTCTACCCACCACCGCATATCTCCGTCAGAGTCAAT
GGAGGCTGGCAGAGCAAATGAGAAGCATTTGGTATTACATGATGGTCTTAACTGTGGGGCCGACAAACAGAACAATGGTACTAGTTTGGCCATGGAGGGGGAACAACCACAACTATGTATAGAAGCAGTTCAGCCCAAAAGGAAAAGAAGGGCAGGCCAATGCAACTTGAGGAAAAGTCTAGCATGGGACAAAGCCTTCT
TCACTAGTGAAGGGGTTCTCAATCCTGAAGAACTATCACTTGTGAACAAGACTTTCAAGAAATCAAATGAGACTGTGTTACCTATTATTGAAGAGGAAGACTCCGTGAGAAGTTCTTTTGGTTCAAACGGCAGCCTTGGGGAAGAAGATCCGGCTTTACAATGTCTCGAAGAAAAACTATTTACAGAGCTCCAAACTTCA
AAGCCTAAACTTGATAATGAAGTTGATAAGATATGCAATGCTGCATCTTGTGAAACTTTTCCTGCTCAACCATTAGGACTTCCACCATCCAATGTAAAACATTTATCTACAGAAAAAAGGCACATTGCCATGAAAGTTCTTGAGACTCCTTCACAGAAAAAGGCTGCCCTTGGACCCCAAGCTCCCTTCACTTCAAAAAG
GTCAAGTGCTAAAGCCACCGAGGAATTGGAAAAGGCAAGCCAGTCAAATGATGCATATGGAAAGAATTGCTCTGCTGCACCTCCAGTAGTTACAAAAAATTTGTCTAAAGgtcataaacgcatgaaatatacacatggaaatgctgtgccagCTAACTTGACCAGGGCTTCCCATCACAGAAACTCGCCAGCAAAGATAA
AAGAAAATTTTGGCATCACTAGTAGATCTGCTCCATCGCCAAATTCTTCTGCATCTGACAATTTGAATTCTGAAAAACGTCCAGCTTCTTCTTCTTGTAGCCTTGAAAAGTTAAGGAAGTCTCTATTTGAAATGACAAAGGAAGGTACACCTGGGACTAAAGACTCAGACAGAAATGGGAATTTTCTTGGCACATCTTCT
GTGCAATTTTGTCATGGGATGCCTGCATTTCATTCTTCAATGGTTTCTGCTACCTCGACAGCCAGTGGTTCATTATTTCTGACCCACACATCTCGTGAGCAGTTTGCGCCCACCACAGGGGCACGTACATCTGCAGATTCAACAGTTTCTCAATTTGGCAAGAACCATGAATTTTGGAACACAATGGCAGGGCACTTGCC
TACAAAAGATGATTCTATAAGCATTGCTGGTAATAGTGTGATTTTTAGTGCCAAGAATCCTCCAAGTAATTCTGGCCATGCTTTGCCTGTAACTTTTACTGCAAAGCCCTCAGCCTCAAGAATTCCTAGAAAAGATGATCATATAAGCACTGCTTCTAATAGCATGGTTTTTAGTGCCAAGGATCCTCCAAGTAATTCTA
GTAATGCTTTGCCTGTCACTTTTGCTGCAAAGCCCTCGGGCTTGAGAATGCCTTCACCAAAACTAGGATTTTTTGATTCGACAAAAGTGATCCGTGCACCATATTCCTCTTCCTTACACAGAAGATCTGCCGGCAATCGTGTCCTTCCACCCAGGGCTATGCCCAGAGGGCCAAGCAGTATTCATCAGGGTAATAGATTG
AAACCCCCAGGTGTTCCTAGTGCAATACCATCCTCCATTTCTGTGAGCAATTTTAGTTTTCCAAACTATAATGCTATTGTACCACCCAGCACTCCAGTGACTTCCAATTTGTTGTCTGATGCTGCCCTGAAGAGTGCTATCACATCCAATCCAGCTCCTCCATTTCAGCCACAGGTTAAACCAGACTCTGAAGTTTCTGG
TTTGGTCTTGTTGGCACAGAAAAAATTTGGTGTTTCTAAGGTTCAGCAAACTTCATCTGTCCAAAGAAGTGATCAGAAAAAGGTCCCTTCAGATGTTTGTACAGATGCCTCATCAACAGTGGAGATTGGTAAAGCTTCGAATGCGAGTAGTACTAACATTCCTCATCCTGGTCATGGACGTGGAATGGCTGTTCTTTCTG
ATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGATAAGGTTGAGAATGGAATCTCT
GATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGCTTGTGTCTTGGCCTGTGAATTTAGAACATCTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTCTGCAAGAGAATGAAATGGAGCCTGAGTCTTGGCGTGTAAGATCAGGACATTTTCCATCCCTAGGTCATGGACGTGAAATGGCTGTTCTTTC
TGATGCCAGGACAAATGATCTGACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGATAAGGTTGAGAATGGAATCT
CTGATTTGGGTTCTAGTTTGCAGCAGGAGAACAAAATGAAGCTTGTGTCTTGGCCTGTGAATTTAGAACATTTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTTTGCAAGAGAATGAAATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAAGGAAATAGAGAAGGTATCT
ATTGGGTTAGACATTGTTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCAGGATGGTGCTTCCTGTCAAGTTTCTCAGGCAAGTATCTCTATGCAAGATGAGGATTTACATGATAATGTTAGAAATGGAAATTCTAATTTGGTTTGTCAGTTGCAGTTGGAAAATGAAATGGAGACTACATCTCAGCCTGTAAA
ATTTGAGCAGTCCCTGGCTACAAGAAAAGGAGAAACACAGAAGGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGATAAGGGTTTGGATGTAGTTCAAAATGAAGTAAATGATAGAGCTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAATCCATATTAT
CTGATGTTACTGATAGCTTGGAATTCAAAACTGGAGTGGTGTTTAATCTTAAAGAGAATGTCACAAATTCTTCCAACAATTATCAAAATAATACCACCGATACGCTTATTACTCCATCCCTATTGTCAGGCTGTCCTCCATGTGAAGCTTTTCCTTCAGTCATAGAACATAAATCTATAGATAAAGATGATTCTTCATTA
CGTAATATGAAATTGTCTGATAGCTCGGTTACGCTAAAAGTGCAAATGATGTCTGGGCTCAAAGTTGCTGGTAATGAAGTTAAATTTAAGGGAAGAAGTCCTTTGGCTACCAATTACGAGCTGCTGAACTCTACTGCTAAGGAAATATCCCAGAAACATGTCAAAGAACAGAGGTGTTCAATGGATGGTTCAGAGAGATT
GACATCCTTGCCTAGGTCATGTCCTGTATTGGATGATACAGCAGAGAAAGAGAACAGCAGGCCAGAAGGGCAAGATGGAGTTGGCATGTCAGAAGTGCATGGTGCAGATGGTCATTATGTAAGCTCTGGAAGAAAGTCAATGGGTAACCTAAGTGTTCAAGTACAGAAAATAAAGAACCCTTTAGATGCTTCGCCATTTT
CAGAAGAGTGGATTGCTGCTATACAAGCTGTGGGTGAGGAATGGCTTCAAATGAAAAGAGGACCAGTGCAGAACTCACCACCAAACAAAATGGTTCCCCAACCAGGTCCATGGTCTCCGGTTCGGCGCCCTAACCAGCAACTAGGTCCATTTGATTGTACTAAATATACAAATGCAGCGGACAGTACTGATGTTTATGAA
GCTGAATAAGATGCCTGTGGTAGGCATGCTTTTCATGTATGGCAGAAGGGTGATCAGTCTTGTACATAGTTCCTAATTACCAATATGTACGAACTATGAAGTTACCATCTGCGGCAAACAAAGGCATGGAATCTGGCTTGCAGGGAACTCTTATTATATGCTATGATGCCCTTATGCCAGGGTCTGTTCCAGTAGAATCT
CCCATGTTTCTTGTTTTGAAAGATTGTGGTCAAGAACGATACAATAGCAGGCTAAAAGTCTGTTGGTGGAGCAGGATCTGATAGTTTGTTGCAAGTGACCTTAGTGTTGATCATTTTAGATATTTTGTGATTTATTCTGTTTACAAACTTGTCTTGTAAGTGAACCCATCTCAGGGCTATTGTAATCTGTTTTCATTAGG
CTTTCCTTTGTTGGCTGAGAAAATTTCTCAGAAGGATCAATTTTTTGAGCATTTCAGAAATATTTCTGCATTCCAGGCTCCTAAGACAATCCAAATTACACAATGCATTGAACGTTGCAGTTGTGTATGGGTACTCTTATTTACTTCTGTACATGAGTGCTTGATGGTGCAGAAGAAATGATGGAATTCTTTTCATAAGT
AGCGGTTTGGTGCACCAGCTACAGGAAATATATCTGGATGTTAAGAAGGTACTTGCAGCAATTGTTCATGATGTCTTTGTAATTTGGATGTATAAAACTGTAGATAATAAGCTTCCTTTAACACATCT



Intron 5 GTATTTTCTC ... CTTTTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A113.726108097
A210.044936355
A312.8651561.376
A412.234025656
A516.9117224.735
A614.2290594.7150
A712.081565213215
A815.951792233.3340
A914.941366165.7240
A1011.421109163.3143



>TRINITY_DN47440_c1_g1_i8 from sample A7 / Example spliced isoform
CTTATATATCTTTCCCGCTCGTGGAGAGCTTTGAATTTCTTCTACCATAAACGGCTCAAAGAGTTTCGTTCAATCTTTTTTCTTTCACGTTTGCTTAGCCCTTTTCAGATCTGCTTTTCAAGTTTTGCCAAAAGAAACACCGGGGTTCGAATCTCGTGGGGAGCTTGGAATGAGAGATCCAGTCGATGGATCACCAAACG
GATGTGGACGGCCTGAGTCTCATAGATGTATCTGCAGAAGATGACAGCCTCTTATCATCGCCCGCTTTAAAGTCGCATAACGATGCCGATCGAACTCTTGATTTCCATTCCTCGCCAGCAACCACATTTCGAGTGCAGCAAAGTGCTCTCGCGTCTACCCACCACCGCATATCTCCGTCAGAGTCAATGGAGGCTGGCAG
AGCAAATGAGAAGCATTTGGTATTACATGATGGTCTTAACTGTGGGGCCGACAAACAGAACAATGGTACTAGTTTGGCCATGGAGGGGGAACAACCACAACTATGTATAGAAGCAGTTCAGCCCAAAAGGAAAAGAAGGGCAGGCCAATGCAACTTGAGGAAAAGTCTAGCATGGGACAAAGCCTTCTTCACTAGTGAAG
GGGTTCTCAATCCTGAAGAACTATCACTTGTGAACAAGACTTTCAAGAAATCAAATGAGACTGTGTTACCTATTATTGAAGAGGAAGACTCCGTGAGAAGTTCTTTTGGTTCAAACGGCAGCCTTGGGGAAGAAGATCCGGCTTTACAATGTCTCGAAGAAAAACTATTTACAGAGCTCCAAACTTCAAAGCCTAAACTT
GATAATGAAGTTGATAAGATATGCAATGCTGCATCTTGTGAAACTTTTCCTGCTCAACCATTAGGACTTCCACCATCCAATGTAAAACATTTATCTACAGAAAAAAGGCACATTGCCATGAAAGTTCTTGAGACTCCTTCACAGAAAAAGGCTGCCCTTGGACCCCAAGCTCCCTTCACTTCAAAAAGGTCAAGTGCTAA
AGCCACCGAGGAATTGGAAAAGGCAAGCCAGTCAAATGATGCATATGGAAAGAATTGCTCTGCTGCACCTCCAGTAGTTACAAAAAATTTGTCTAAAGGTCATAAACGCATGAAATATACACATGGAAATGCTGTGCCAGCTAACTTGACCAGGGCTTCCCATCACAGAAACTCGCCAGCAAAGATAAAAGAAAATTTTG
GCATCACTAGTAGATCTGCTCCATCGCCAAATTCTTCTGCATCTGACAATTTGAATTCTGAAAAACGTCCAGCTTCTTCTTCTTGTAGCCTTGAAAAGTTAAGGAAGTCTCTATTTGAAATGACAAAGGAAGGTACACCTGGGACTAAAGACTCAGACAGAAATGGGAATTTTCTTGGCACATCTTCTGTGCAATTTTGT
CATGGGATGCCTGCATTTCATTCTTCAATGGTTTCTGCTACCTCGACAGCCAGTGGTTCATTATTTCTGACCCACACATCTCGTGAGCAGTTTGCGCCCACCACAGGGGCACGTACATCTGCAGATTCAACAGTTTCTCAATTTGGCAAGAACCATGAATTTTGGAACACAATGGCAGGGCACTTGCCTACAAAAGATGA
TTCTATAAGCATTGCTGGTAATAGTGTGATTTTTAGTGCCAAGAATCCTCCAAGTAATTCTGGCCATGCTTTGCCTGTAACTTTTACTGCAAAGCCCTCAGCCTCAAGAATTCCTAGAAAAGATGATCATATAAGCACTGCTTCTAATAGCATGGTTTTTAGTGCCAAGGATCCTCCAAGTAATTCTAGTAATGCTTTGC
CTGTCACTTTTGCTGCAAAGCCCTCGGGCTTGAGAATGCCTTCACCAAAACTAGGATTTTTTGATTCGACAAAAGTGATCCGTGCACCATATTCCTCTTCCTTACACAGAAGATCTGCCGGCAATCGTGTCCTTCCACCCAGGGCTATGCCCAGAGGGCCAAGCAGTATTCATCAGGGTAATAGATTGAAACCCCCAGGT
GTTCCTAGTGCAATACCATCCTCCATTTCTGTGAGCAATTTTAGTTTTCCAAACTATAATGCTATTGTACCACCCAGCACTCCAGTGACTTCCAATTTGTTGTCTGATGCTGCCCTGAAGAGTGCTATCACATCCAATCCAGCTCCTCCATTTCAGCCACAGGTTAAACCAGACTCTGAAGTTTCTGGTTTGGTCTTGTT
GGCACAGAAAAAATTTGGTGTTTCTAAGGTTCAACAAACTTCATCTGTCCAAAGAAGTGATCAGAAAAAGGTCCCTTCAGATGTTTGTACAGATGCCTCATCAACAGTGGAGATTGGTAAAGCTTCGAATGCGAGTAGTACTAACATTCCTCATCCTGGTCATGGACGTGGAATGGCTGTTCTTTCTGATGCCAGGACAA
ATGATCTGACCAAGCCTTTACAATCTGAACAATTGTCATCCCTCTCATGCAGCAAAATAACAAAAATATCTGAAAAGTTCCAGCTTGAGACTTGTCCTGATTTAATTCGAAATGAAGAACACTATGGACTTTCAAACAGTAATTCAGAAGGAATCATGAGGGATGATAAGGTTGAGAATGGAATCTCTGATTTGGGTTCT
AGTTTGCAGCAGGAGAACAAAATGAAGCTCGTGTCTTGGCCTGTGAATTTAGAACATCTGCCATCTCCAAGACGGAACTCTGGTTTGTGTTCTACTCTGCAAGAGAATGAAATGGAGCCTGTGTCTTGGCATGTAAGATCAGAACATTTGCCATCCCTAGGCCAGAAGGAAATAGAGAAGGTATCTATTGGGTTAGACAT
TGTTTCAACCACTGGTTCCGTTTCAGTTCAGATTGAAAAGCAGGATGGTGCTTCCTGTCAAGTTTCTCAGGCAAGTATCTCTATGCAAGATGAGGATTTACATGATAATGTTAGAAATGGAAATTCTAATTTGGTTTGTCAGTTGCAGTTGGAAAATGAAATGGAGACTACATCTCAGCCTGTAAAATTTGAGCAGTCCC
TGGCTACAAGAAAAGGAGAAACACAGAAGGTAACTGAAGAATTCCTCTTTTCTGAAGCCTCTAGTCTTGATAAGGGTTTGGATGTAGTTCAAAATGAAGTAAATGATAGAGCTTGCACCAAAGCTTGCCAAGGAAGTATGAATATCCAGAGTGACTATTCAAATGACAAGGTCAAATCCATATTATCTGATGTTACTGAT
AGCTTGGAATTCAAAACTGGAGTGGTGTTTAATCTTAAAGAGAATGTCACAAATTCTTCCAACAATTATCAAAATAATACCACCGATACGCTTATTACTCCATCCCTATTGTCAGGCTGTCCTCCATGTGAAGCTTTTCCTTCAGTCATAGAACATAAATCTATAGATAAAGATGATTCTTCATTACGTAATATGAAATT
GTCTGATAGCTCGGTTACGCTAAAAGTGCAAATGATGTCTGGGCTCAAAGTTGCTGGTAATGAAGTTAAATTTAAGGGAAGAAGTCCTTTGGCTACCAATTACGAGCTGCTGAACTCTACTGCTAAGGAAATATCCCAGAAACATGTCAAAGAACAGAGGTGTTCAATGGATGGTTCAGAGAGATTGACATCCTTGCCTA
GGTCATGTCCTGTATTGGATGATACAGCAGAGAAAGAGAACAGCAGGCCAGAAGGGCAAGATGGAGTTGGCATGTCAGAAGTGCATGGTGCAGATGGTCATTATGTAAGCTCTGGAAGAAAGTCAATGGGTAACCTAAGTGTTCAAGTACAGAAAATAAAGAACCCTTTAGATGCTTCGCCATTTTCAGAAGAGTGGATT
GCTGCTATACAAGCTGTGGGTGAGGAATGGCTTCAAATGAAAAGAGGACCAGTGCAGAACTCACCACCAAACAAAATGGTTCCCCAACCAGGTCCATGGTCTCCGGTTCGGCGCCCTAACCAGCAACTAGGTCCATTTGATTGTACTAAATATACAAATGCAGCGGACAGTACTGATGTTTATGAAGCTGAATAAGATGC
CTGTGGTAGGCATGCTTTTCATGTATGGCAGAAGGGTGATCAGTCTTGTACATAGTTCCTAATTACCAATATGTACGAACTATGAAGTTACCATCTGCGGCAAACAAAGGCATGGAATCTGGCTTGCAGGGAACTCTTATTATATGCTATGATGCCCTTATGCCAGGGTCTGTTCCAGTAGAATCTCCCATGTTTCTTGT
TTTGAAAGATTGTGGTCAAGAACGATACAATAGCAGGCTAAAAGTCTGTTGGTGGAGCAGGATCTGATAGTTTGTTGCAAGTGACCTTAGTGTTGATCATTTTAGATATTTTGTGATTTATTCTGTTTACAAACTTGTCTTGTAAGTGAACCCATCTCAGGGCTATTGTAATCTGTTTTCATTAGGCTTTCCTTTGTTGG
CTGAGAAAATTTCTCAGAAGGATCAATTTTTTGAGCATTTCAGAAATATTTCTGCATTCCAGGCTCCTAAGACAATCCAAATTACACAATGCATTGAACGTTGCAGTTGTGTATGGGTACTCTTATTTACTTCTGTACATGAGTGCTTGATGGTGCAGAAGAAATGATGGAATTCTTTTCATAAGTAGCGGTTTGGTGCA
CCAGCTACAGGAAATATATCTGGATGTTAAGAAGGTACTTGCAGCAATTGTTCATGATGTCTTTGTAATTTGGATGTATAAAACTGTAGATAATAAGCTTCCTTTAACACATCTAATTTAAAAAAGC


>TRINITY_DN47440_c1_g1_i3 from sample A7 / Example unspliced isoform:
CAACCAGGTCCATGGTCTCCGgtattttctcatccactaggttgtgtagtaacaaaatgtatgtaaacataattagtaaacactgagctgaatattccagagagatgactaatgtacctaagattactacaccctgttggtttagaatccactattttagtgctgatgtagccctttttgcagGTTCGGCGCCCTAACCA
GCAACTAGGTCCATTTGATTGTACTAAATATACAAATGCAGCGGACAGTACTGATGTTTATGAAGCTGAATAAGATGCCTGTGGTAGGCATGCTTTTCATGTATGGCAGAAGGGTGATCAGTCTTGTACATAGTTCCTAATTACCAATATGTACGAACTATGAAGTTACCATCTGCGGCAAACAAAGGCATGGAATCTGG
CTTGCAGGGAACTCTTATTATATGCTATGATGCCCTTATGCCAGGGTCTGTTCCAGTAGAATCTCCCATGTTTCTTGTTTTGAAAGATTGTGGTCAAGAACGATACAATAGCAGGCTAAAAGTCTGTTGGTGGAGCAGGATCTGATAGTTTGTTGCAAGTGACCTTAGTGTTGATCATTTTAGATATTTTGTGATTTATT
CTGTTTACAAACTTGTCTTGTAAGTGAACCCATCTCAGGGCTATTGTAATCTGTTTTCATTAGGCTTTCCTTTGTTGGCTGAGAAAATTTCTCAGAAGGATCAATTTTTTGAGCATTTCAGAAATATTTCTGCATTCCAGGCTCCTAAGACAATCCAAATTACACAATGCATTGAACGTTGCAGTTGTGTATGGGTACTC
TTATTTACTTCTGTACATGAGTGCTTGATGGTGCAGAAGAAATGATGGAATTCTTTTCATAAGTAGCGGTTTGGTGCACCAGCTACAGGAAATATATCTGGATGTTAAGAAGGTACTTGCAGCAATTGTTCATGATGTCTTTGTAATTTGGATGTATAAAACTGTAGATAATAAGCTTCCTTTAACACATCTAATTTAAA
AAAGC