sp|Q680K2|GPPL1_ARATH ----> Haloacid dehalogenase-like hydrolase domain-containing protein At4g39970

Intron 1 GTGGGTTCTC ... TGTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.13	13	2	3
A2	31.71	14	5	8
A3	34.69	16	3	14
A4	0	7	0	0
A5	25	6	2	2
A6	25.64	29	3	17
A7	25	39	9	17
A8	18.37	20	2	7
A9	22.92	37	6	16
A10	26.98	23	2	15

>TRINITY_DN107592_c1_g1_i8 from sample A3 / Example spliced isoform

CAGGACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATC
AACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCAC
TTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGA
ATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGT

>TRINITY_DN107592_c1_g1_i6 from sample A3 / Example unspliced isoform:

CAGGACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATC
AACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCAC
TTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGgtgggttctctcctcagttcataattcatatctaatcatttctaagtcccattgtttctgaaaccccgccttccaccttgttttgcagGTACTTTA
AGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTT
GGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGT

Intron 2 GTCGGTATCT ... TCCTCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40.74	8	2	9
A2	52.01	6.5	6	8
A3	30.35	12.5	7	4
A4	26.66	6	0	4.5
A5	57.14	3	6	2
A6	8	31.8	0	6
A7	28.26	27.3	12	9.5
A8	30.46	43.5	13	24.5
A9	9.66	35.8	5	2.5
A10	19.14	22.5	6	4.5

>TRINITY_DN105905_c0_g1_i3 from sample A2 / Example spliced isoform

GTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTA
CTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACAC
CTTGTCAACAAAAGATCAAGACTTCAAAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGC

>TRINITY_DN105905_c0_g1_i4 from sample A2 / Example unspliced isoform:

GTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTA
CTTGGGCTGCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCTGGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTTGACATCTATAACATGACCAGACTAGAGGTGGAATAGTTTAATTCAAACAGTCATGATTTTGGAAGGCCACCAATATTTTCTA
AAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGCTTCTGAAATTGGGGATAAAACAAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGGTATTCGAATAAAAAGAAAAACATCATATATGCATGTCATTTATGATTTAACAGATCCAAGAAAGGTAACATGTTAT
CACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGAGCAATTCTAAATAACAAGCTACATTTCTGGGTGCCCTTGACATTGAAAGAAAAGCCTATCATCCAAATTTATACCCTGATTGAAATGCTTACTTGCTTACTTGTGAACTGCTGGATGTCATCAATAGTTGCAAGAATCCCATATTAGATAATTA
CTGTTGCCCCTTTTTTTAGCAATTTTTCTAACTGAATCCAAATTTGATGCTCCAACCTATGCTGGTAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGgtcggtatctttcaaaaacaaacttactaggttttcataatgcaccaaacaaaaatttaagcacctcgtgcttttg
ggaatatattcatgcaatgtttccgtcatttcaatacttgagttttcttgatgttgttcctctacagAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGTATGAAATAAAATTTTAGCCATGACAATCTCCTCCAAAAAATATTTGTAATCCTTTATTTTATATTTGCACC
ATTATGCTGCTGCTTATATTGGGGTATTATAAGGTTGTTACCTTCATAGTTTGATGGGAAATCTGTGCATCTTTATTGATCAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAG

Intron 3 GTTAAGAATT ... TGTGATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.46	4	4	1
A2	60	4	4	8
A3	61.54	5	11	5
A4	57.14	3	3	5
A5	48.15	7	7	6
A6	80	1	6	2
A7	50	3	5	1
A8	60	1	0	3
A9	74.19	4	6	17
A10	55.56	6	12	3

>TRINITY_DN96792_c0_g1_i1 from sample A5 / Example spliced isoform

GGCGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCA
CGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTG
ATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCA
AACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGTATGATAACTAGCTTATTATTCCCTCACAATCTTCTCTGGACCAAGGCAGT
AATCTGCCTAATAATAAAAAATTCCCCATAATGTAGATACATTAGTCAGGCAGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGCTTTCAACAGCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCTGGTTCTGTATCTTGAATTGTA
TCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTTGACATCTATAACATGACCAGACTAGAGGTGGAATAGTTTAATTCAAACAGTCATGATTTTGGAAGGCCACCAATATTTTCTAAAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGCTTCTGAAATTGGGGATAAAAC
AAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGGTATTCGAATAAAAAGAAAAACATCATATATGCATGTCATTTATGATTTAACAGATCCAAGAAAGGTAACATGTTATCACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGAGCAATTCTAAATAACAAGCTA
CATTTCTGGGTGCCCTTGACATTGAAAGAAAAGCCTATCATCCAAATTTATACCCTGATTGAAATGCTTACTTGCTTACTTGTGAACTGCTGGATGTCATCAATAGTTGCAAGAATCCCATATTAGATAATTACTGTTGCCCCTTTTTTTAGCAATTTTTCTAACTGAATCCAAATTTGATGCTCCAACCTATGCTGGTA
GGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAGACTTCAAAGGTGCAAAAGCAAT
TTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGAC
TTGATTTTCAATTTGGA

>TRINITY_DN96792_c0_g1_i2 from sample A5 / Example unspliced isoform:

GGCGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCA
CGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTG
ATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCA
AACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGTATGATAACTAGCTTATTATTCCCTCACAATCTTCTCTGGACCAAGGCAGT
AATCTGCCTAATAATAAAAAATTCCCCATAATGTAGAGATACATTAGTCAGGCAgttaagaattacatttccaaattggttataacaagcgttacattcacatgcgatctaatcctatatggcaacagtagacagtgtccacgtctcagaaacccaggcctgaatcaattggcaagcacggcttaatacaatctctttgt
tctgtgatgtagGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGTACTGCATCCCTTGAGTCTATATTCTATTACCGATGTTTATTGGTTTCTCTGATGTTACAGCCTGC

Intron 4 GTCGGTATCT ... TAGAGGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	2	6
A2	78.95	2	6	9
A3	85.71	1	7	5
A4	100	0	0	2
A5	100	0	6	1
A6	33.33	1	0	1
A7	56.76	8	12	9
A8	41.67	14	13	7
A9	25.93	10	5	2
A10	45.45	6	6	4

>TRINITY_DN41381_c0_g2_i1 from sample A6 / Example spliced isoform

TCAGTATATATGAGTCAGGCTTACTTCCTTTCCATTGCTTTCCATGACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGT
CTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAG

>TRINITY_DN41381_c0_g2_i3 from sample A6 / Example unspliced isoform:

AATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCA
ATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCT
GCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCA
GAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGC
AGTTAATGGATGAAGCCAGGGCAGCTGTATGATAACTAGCTTATTATTCCCTCACAATCTTCTCTGGACCAAGGCAGTAATCTGCCTAATAATAAAAAATTCCCCATAATGTAGAGATACATTAGTCAGGCAGTTAAGAATTACATTTCCAAATTGGTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGG
CAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGTACTGCATCCCTTGAGTCTATATTCTATTACCGA
TGTTTATTGGTTTCTCTGATGTTACAGCCTGCATTCTTTTCCGTAATAGATTTTCCTCTTCTGCAGGAGCGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTTGACATCTATAACATGACCAGACTAGAGGTGGAATAGTTTAATTCAAACAGTCATGATTT
TGGAAGGCCACCAATATTTTCTAAAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGCTTCTGAAATTGGGGATAAAACAAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGGTATTCGAATAAAAAGAAAAACATCATATATGCATGTCATTTATGATTTAACAG
ATCCAAGAAAGGTAACATGTTATCACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGAGCAATTCTAAATAACAAGCTACATTTCTGGGTGCCCTTGACATTGAAAGAAAAGCCTATCATCCAAATTTATACCCTGATTGAAATGCTTACTTGCTTACTTGTGAACTGCTGGATGTCATCAATAGTTGC
AAGAATCCCATATTAGATAATTACTGTTGCCCCTTATTTTTAGCAATTTTTCTAACTGAATCCAAATTTGATGCTCCAACCTATGCTGGTAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGgtcggtatctttcaaaaacaaacttactaggttttcataatgcaccaaacaa
aaatttaagcacctcgtgcttttgggaatatattcatgcaatgttttccgtcatttcaatacttgagttttcttgatgttgttcctctacagagattgggaaagtcagctaaaaattgtcttgttgtagaggacagCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCA
ACAAAAGATCAAG

Intron 5 GTATGATAAC ... CTGGACCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.36	5	3	2
A2	67	1.7	3	2
A3	45.83	8.7	2	4
A4	29.07	5.3	2	2
A5	50	2	2	2
A6	3.35	18.3	0	1
A7	27.39	19.7	4	4
A8	15.72	23.7	5	1
A9	12.82	27.3	3	3
A10	6.39	21.7	0	2

>TRINITY_DN43144_c0_g1_i4 from sample A7 / Example spliced isoform

CTTTCCTTAATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAA
ATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGG
CGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGG
TACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACT
TGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCG
TTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAGACTTCAGAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACT
TCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAATGATAGTACTTTCTTTTTCTCTTCGATTAGCAAAACC

>TRINITY_DN43144_c0_g1_i5 from sample A7 / Example unspliced isoform:

AGCTTGAGGTTGCATACTTGAATTCGAAGTTATTTGTGTGAGCTTCAGATATGCTTTATGGATCTTAGCAGTTCAATGACCCTATCAAACCCAGGTTTATATGGGTCTGCACTGCTCTCTATACTCAAATGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGC
AGTTAATGGATGAAGCCAGGGCAGCTgtatgataactagcttattattccctcacaatcttctctggaccaagGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCT
GGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTTGACATCTATAACATGACCAGACTAGAGGTGGAATAGTTTAATTCAAACAGTCATGATTTTGGAAGGCCACCAATATTTTCTAAAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGC
TTCTGAAATTGGGGATAAAACAAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGGTATTCGAATAAAAAGAAAAACATCATATATGCATGTCATTTATGATTTAACAGATCCAAGAAAGGTAACATGTTATCACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGA
GCAATTCTAAATAACAAGCTACAT

Intron 6 GTATGATAAC ... TGTGATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.52	5.6	5.8	1.7
A2	82.1	2	10.5	7.6
A3	41.06	10.5	4	7.1
A4	39.2	6	2.5	5
A5	71.35	2.1	6	4.6
A6	8.32	20.2	0.7	2.7
A7	27.52	23	11.9	1.6
A8	18.18	25.5	3.9	5.2
A9	32.5	29.8	8.7	17.6
A10	21.85	23.8	7.1	4.4

>TRINITY_DN107592_c1_g1_i13 from sample A3 / Example spliced isoform

ATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTG
CAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAGACTTCAGAGGTGCAAAAGCA
ATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAG
ACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAATGAT

>TRINITY_DN107592_c1_g1_i11 from sample A3 / Example unspliced isoform:

ATGGAACAATTTCTTTTTCCATTTCTGTAGGAGTTATATTCCAGTTTAAAATATGTGAAATTTCTCTTTGCTTACCAGGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTgtatgataactagcttattattccctcacaatcttctctggaccaaggcagtaatctgcctaataataaaa
aattccccataatgtagagatacattagtcaggcagttaagaattacatttccaaattggttataacaagcgttacattcacatgcgatctaatcctatatggcaacagtagacagtgtccacgtctcagaaacccaggcctgaatcaattggcaagcacggcttaatacaatctctttgttctgtgatgtagGGAATTA
AACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCTGGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTT

Intron 7 GTAAAGCCAA ... TGTGATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.41	6	3	2
A2	44	7	4	7
A3	47.62	11	11	9
A4	63.64	2	2	5
A5	71.43	1	2	3
A6	14.29	9	0	3
A7	16.13	13	5	0
A8	30.77	9	5	3
A9	50	10	5	15
A10	17.65	14	1	5

>TRINITY_DN107592_c1_g1_i8 from sample A3 / Example spliced isoform

CAGGACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATC
AACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCAC
TTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGA
ATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGT

>TRINITY_DN107592_c1_g1_i3 from sample A3 / Example unspliced isoform:

CAGGACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATC
AACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCAC
TTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGA
TGAGACTTGACTGCTAACAAGTCACTCTTTTATTGACTTCCACGGGATCACTGCATGTATACATAATATTTGCTCAATAGAATTCAATCCCATGGAAAGTATCTGTATATATGAGTCAGGCTTACTTCCTTTCCATTGCTTTCCATGACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAgtaa
agccaaggccaggggtattgcagttaatggatgaagccagggcagctgtatgataactagcttattattccctcacaatcttctctggaccaaggcagtaatctgcctaataataaaaaattccccataatgtagagatacattagtcaggcagttaagaattacatttccaaattggttataacaagcgttacattcac
atgcgatctaatcctatatggcaacagtagacagtgtccacgtctcagaaacccaggcctgaatcaattggcaagcacggcttaatacaatctctttgttctgtgatgtagGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACA
GCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCTGGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTT

Intron 8 GTAAGTTGCT ... TGCTTACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.72	4.6	7	1.7
A2	71.45	4.4	7	3.1
A3	63.05	5.4	7	3.6
A4	48.71	4.6	4	5.9
A5	26.19	3.3	1	2
A6	45.71	13.1	8	3.3
A7	58.68	12.4	15	5.9
A8	46.24	11.4	9	1.4
A9	45.08	15.1	10	3.9
A10	51.89	13.4	11	2.4

>TRINITY_DN43144_c0_g1_i9 from sample A7 / Example spliced isoform

CTTTCCTTAATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAA
ATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGG
CGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGG
TACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGTATGATAACT
AGCTTATTATTCCCTCACAATCTTCTCTGGACCAAGGCAGTAATCTGCCTAATAATAAAAAATTCCCCATAATGTAGAGATACATTAGTCAGGCAGTTAAGAATTACATTTCCAAATTGGTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGC
CTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGTACTGCATCCCTTGAGTCTATATTCTATTACCGATGTTTATTGGTTTCTCTGATGTTACAGCCTGCATTCT
TTTCCGTAATAGATTTTCCTCTTCTGCAGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAG

>TRINITY_DN43144_c0_g1_i1 from sample A7 / Example unspliced isoform:

CTTTCCTTAATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAA
ATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGG
CGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGG
TACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAgtaagttgcttgcttcctaaattttatcctgtatctgtgttataatggaacaatttcttttt
ccatttctgtaggagttatattccagtttaaaatatgtgaaatttctctttgcttaccagGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGTATGATAACTAGCTTATTATTCCCTCACAATCTTCTCTGGACCAAGGCAGTAATCTGCCTAATAATAAAAAATTCCCCATAATGTAGA
GATACATTAGTCAGGCAGTTAAGAATTACATTTCCAAATTGGTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCG
CGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGTACTGCATCCCTTGAGTCTATATTCTATTACCGATGTTTATTGGTTTCTCTGATGTTACAGCCTGCATTCTTTTCCGTAATAGATTTTCCTCTTCTGCAGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAG

Intron 9 GTGATGATGT ... CTTGTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	7	10
A2	100	0	10	13
A3	100	0	7	17
A4	80	1	0	8
A5	100	0	3	4
A6	92.98	2	17	36
A7	94.2	2	32	33
A8	96	2	54	42
A9	98.04	1	46	54
A10	96.83	1	32	29

>TRINITY_DN43144_c0_g1_i10 from sample A7 / Example spliced isoform

AGCTTGAGGTTGCATACTTGAATTCGAAGTTATTTGTGTGAGCTTCAGATATGCTTTATGGATCTTAGCAGTTCAATGACCCTATCAAACCCAGGTTTATATGGGTCTGCACTGCTCTCTATACTCAAATGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGC
AGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGAGGACAGCGTTATTGGTTTAC

>TRINITY_DN43144_c0_g1_i6 from sample A7 / Example unspliced isoform:

AGCTTGAGGTTGCATACTTGAATTCGAAGTTATTTGTGTGAGCTTCAGATATGCTTTATGGATCTTAGCAGTTCAATGACCCTATCAAACCCAGGTTTATATGGGTCTGCACTGCTCTCTATACTCAAATGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGC
AGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGgtgatgatgtaaagaataagaagcctgatccttcaatttatttagaagctgcaaagagattggga
aagtcagctaaaaattgtcttgttgtagAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAGACTTCAGAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGT
TTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAATGATAGTACTTTCTTTTTCTCTTCGATT
AGCAAAACC

Intron 10 GTCTGTTTTC ... ATGCTGGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.13	6.3	3.6	6
A2	27.46	8.5	1.8	4
A3	47.38	6.2	3	8
A4	96	0.1	1.4	2
A5	55	2.7	1	5
A6	16.34	14.5	4.6	1
A7	16.11	27.2	0	9
A8	11.34	46.2	1.6	9
A9	13.45	40.1	5.6	6
A10	8.29	27.5	2.8	2

>TRINITY_DN105905_c0_g1_i3 from sample A2 / Example spliced isoform

GTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTA
CTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACAC
CTTGTCAACAAAAGATCAAGACTTCAAAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGC

>TRINITY_DN105905_c0_g1_i4 from sample A2 / Example unspliced isoform:

GTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTA
CTTGGGCTGCTAGATTGCTTCCTTGCAGgtctgttttcagcaatctggttctgtatcttgaattgtatcttagtattctttggttcctgattgaattgtcgaacttattgtgcactttgacatctataacatgaccagactagaggtggaatagtttaattcaaacagtcatgattttggaaggccaccaatattttcta
aaatctctacaagacaataatccaatcgatttaataattgtaatgcttctgaaattggggataaaacaaaggactttgatccctgtgagagatcttgtgttcaaaaagacccctattgttctggtattcgaataaaaagaaaaacatcatatatgcatgtcatttatgatttaacagatccaagaaaggtaacatgttat
cactgctaaaaaacaacaataacatacagcaactgaaaagtagggagcaattctaaataacaagctacatttctgggtgcccttgacattgaaagaaaagcctatcatccaaatttataccctgattgaaatgcttacttgcttacttgtgaactgctggatgtcatcaatagttgcaagaatcccatattagataatta
ctgttgcccctttttttagcaatttttctaactgaatccaaatttgatgctccaacctatgctggtagGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGGTCGGTATCTTTCAAAAACAAACTTACTAGGTTTTCATAATGCACCAAACAAAAATTTAAGCACCTCGTGCTTTTG
GGAATATATTCATGCAATGTTTCCGTCATTTCAATACTTGAGTTTTCTTGATGTTGTTCCTCTACAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGTATGAAATAAAATTTTAGCCATGACAATCTCCTCCAAAAAATATTTGTAATCCTTTATTTTATATTTGCACC
ATTATGCTGCTGCTTATATTGGGGTATTATAAGGTTGTTACCTTCATAGTTTGATGGGAAATCTGTGCATCTTTATTGATCAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAG

Intron 11 GTGTGTTCAA ... TATTGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.76	12.9	0.8	4.3
A2	8.51	13.7	3.6	1.3
A3	9.75	11.3	0	1.3
A4	60.32	5.7	2.8	8.6
A5	25.03	7.9	0	2.6
A6	20.6	25.7	2.8	6.6
A7	8.23	48.4	5.7	4.3
A8	12.85	67.3	5.1	10.6
A9	15.98	55.6	3.4	10.9
A10	20.6	32.9	0	8.6

>TRINITY_DN91300_c0_g1_i1 from sample A4 / Example spliced isoform

ATTCAAACAGTCATGATTTTGGAAGGCCACCAATATTTTCTAAAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGCTTCTGAAATTGGGGATAAAACAAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGGTATTCGAATAAAAAGAAAAACATCATATATGCAT
GTCATTTATGATTTAACAGATCCAAGAAAGGTAACATGTTATCACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGAGCAATTCTAAATAACAAGCTACATTTCTGGGTGCCCTTGACATTGAAAGAAAAGCCTATCATCCAAATTTATACCCTGATTGAAATGCTTACTTGCTTACTTGTGAACTGCT
GGATGTCATCAATAGTTGCAAGAATCCCATATTAGATAATTACTGTTGCCCCTTATTTTTAGCAATTTTTCTAACTGAATCCAAATTTGATGCTCCAACCTATGCTGGTAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTT
GTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAGACTTCAAAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGA
TGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAA

>TRINITY_DN91300_c0_g1_i2 from sample A4 / Example unspliced isoform:

CGGTATCTTTCAAAAACAAACTTACTAGGTTTTCATAATGCACCAAACAAAAATTTAAGCACCTCGTGCTTTTGGGAATATATTCATGCAATGTTTTCCGTCATTTCAATACTTGAGTTTTCTTGATGTTGTTCCTCTACAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTAC
AGGTATGAAATAAAATTTTAGCCATGACAATCTCCTCCAAAAAATATTTGTAATCCTTTATTTTATATTTGCACCATTATGCTGCTGCTTATATTGGGGTATTATAAGGTTGTTACCTTCATAGTTTGATGGGAAATCTGTGCATCTTTATTGATCAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACC
TTGTCAACAAAAGATCAAgtgtgttcaattattattgcgttctcctctttaagtcatatttatgaagctgcagaaagaaaaatcagatattaacatcaaaggcctgagtatggtttaaactttttattgtgtagGACTTCAAAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCA
CTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAA

Intron 12 GCAGTAATCT ... TGTGATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60	2	5	1
A2	80.95	2	9	8
A3	52.94	4	4	5
A4	65.16	2	2.5	5
A5	73.33	2	5	6
A6	66.67	1	2	2
A7	57.89	4	10	1
A8	60	1	0	3
A9	79.31	3	6	17
A10	75	2	9	3

>TRINITY_DN43144_c0_g1_i5 from sample A7 / Example spliced isoform

AGCTTGAGGTTGCATACTTGAATTCGAAGTTATTTGTGTGAGCTTCAGATATGCTTTATGGATCTTAGCAGTTCAATGACCCTATCAAACCCAGGTTTATATGGGTCTGCACTGCTCTCTATACTCAAATGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGC
AGTTAATGGATGAAGCCAGGGCAGCTGTATGATAACTAGCTTATTATTCCCTCACAATCTTCTCTGGACCAAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCT
GGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTTGACATCTATAACATGACCAGACTAGAGGTGGAATAGTTTAATTCAAACAGTCATGATTTTGGAAGGCCACCAATATTTTCTAAAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGC
TTCTGAAATTGGGGATAAAACAAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGGTATTCGAATAAAAAGAAAAACATCATATATGCATGTCATTTATGATTTAACAGATCCAAGAAAGGTAACATGTTATCACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGA
GCAATTCTAAATAACAAGCTACAT

>TRINITY_DN43144_c0_g1_i9 from sample A7 / Example unspliced isoform:

CTTTCCTTAATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAA
ATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGG
CGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGG
TACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGTATGATAACT
AGCTTATTATTCCCTCACAATCTTCTCTGGACCAAGgcagtaatctgcctaataataaaaaattccccataatgtagagatacattagtcaggcagttaagaattacatttccaaattggttataacaagcgttacattcacatgcgatctaatcctatatggcaacagtagacagtgtccacgtctcagaaacccaggc
ctgaatcaattggcaagcacggcttaatacaatctctttgttctgtgatgtagGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGTACTGCATCCCTTGAGTCTATATTCTATTACCGATGTTTATTGGTTTCTCTGATGTTACAGCCTGCATTCT
TTTCCGTAATAGATTTTCCTCTTCTGCAGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAG

Intron 13 GTAATACTCT ... TCTTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.56	2	2	2.7
A2	94.44	0.3	5	0.3
A3	88.89	1	6	1
A4	72.73	3	8	4.3
A5	71.43	2	5	0.3
A6	21.79	7.7	2	0.7
A7	47.62	3.3	3	0
A8	40	6	3	0
A9	66.88	8.3	16	1
A10	54.29	5.3	6	0.3

>TRINITY_DN43144_c0_g1_i10 from sample A7 / Example spliced isoform

AGCTTGAGGTTGCATACTTGAATTCGAAGTTATTTGTGTGAGCTTCAGATATGCTTTATGGATCTTAGCAGTTCAATGACCCTATCAAACCCAGGTTTATATGGGTCTGCACTGCTCTCTATACTCAAATGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGC
AGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGAGGACAGCGTTATTGGTTTAC

>TRINITY_DN43144_c0_g1_i1 from sample A7 / Example unspliced isoform:

CTTTCCTTAATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAA
ATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGG
CGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGG
TACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAGTTGCTTGCTTCCTAAATTTTATCCTGTATCTGTGTTATAATGGAACAATTTCTTTTT
CCATTTCTGTAGGAGTTATATTCCAGTTTAAAATATGTGAAATTTCTCTTTGCTTACCAGGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGTATGATAACTAGCTTATTATTCCCTCACAATCTTCTCTGGACCAAGGCAGTAATCTGCCTAATAATAAAAAATTCCCCATAATGTAGA
GATACATTAGTCAGGCAGTTAAGAATTACATTTCCAAATTGGTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCG
CGGCTACAAAAAGCTCAgtaatactctgtcttgaaaatctacttgggctggtactgcatcccttgagtctatattctattaccgatgtttattggtttctctgatgttacagcctgcattcttttccgtaatagattttcctcttctgcagGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAG

Intron 14 GTATGAAATA ... TATTGATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	51.9	7.3	2.5	5.7
A2	34.49	9	8.1	1.6
A3	10.5	11.1	1.6	1
A4	41.34	5.2	1.6	2.1
A5	11.44	4.2	0.8	0.6
A6	39.87	20.9	0.8	5.5
A7	31.41	23.2	2.4	5.6
A8	32.07	35.1	5.7	9.9
A9	28.54	32.1	0	5.7
A10	8.19	18	6.4	1.5

>TRINITY_DN105905_c0_g1_i3 from sample A2 / Example spliced isoform

GTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTA
CTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACAC
CTTGTCAACAAAAGATCAAGACTTCAAAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGC

>TRINITY_DN105905_c0_g1_i4 from sample A2 / Example unspliced isoform:

GTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTA
CTTGGGCTGCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCTGGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTTGACATCTATAACATGACCAGACTAGAGGTGGAATAGTTTAATTCAAACAGTCATGATTTTGGAAGGCCACCAATATTTTCTA
AAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGCTTCTGAAATTGGGGATAAAACAAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGGTATTCGAATAAAAAGAAAAACATCATATATGCATGTCATTTATGATTTAACAGATCCAAGAAAGGTAACATGTTAT
CACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGAGCAATTCTAAATAACAAGCTACATTTCTGGGTGCCCTTGACATTGAAAGAAAAGCCTATCATCCAAATTTATACCCTGATTGAAATGCTTACTTGCTTACTTGTGAACTGCTGGATGTCATCAATAGTTGCAAGAATCCCATATTAGATAATTA
CTGTTGCCCCTTTTTTTAGCAATTTTTCTAACTGAATCCAAATTTGATGCTCCAACCTATGCTGGTAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGGTCGGTATCTTTCAAAAACAAACTTACTAGGTTTTCATAATGCACCAAACAAAAATTTAAGCACCTCGTGCTTTTG
GGAATATATTCATGCAATGTTTCCGTCATTTCAATACTTGAGTTTTCTTGATGTTGTTCCTCTACAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGgtatgaaataaaattttagccatgacaatctcctccaaaaaatatttgtaatcctttattttatatttgcacc
attatgctgctgcttatattggggtattataaggttgttaccttcatagtttgatgggaaatctgtgcatctttattgatcagGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAG

Intron 15 GTGATGATGT ... TAGAGGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.05	1.7	6	9.7
A2	85.04	2.3	7.3	13
A3	83.66	3.3	6	16
A4	80.91	1.7	0.3	7.3
A5	92.5	0.3	3	3.8
A6	82.5	7	15.7	32.8
A7	74.89	11.3	23.7	31.5
A8	70.08	21.3	42	44.3
A9	78.54	14	36.7	49
A10	72.63	12	26	28

>TRINITY_DN39194_c0_g1_i8 from sample A8 / Example spliced isoform

AGGTTCCTCCTTTCCTTAATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTT
AAACACCAAATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTC
CTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAA
ATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGG
AATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAG

>TRINITY_DN39194_c0_g1_i6 from sample A8 / Example unspliced isoform:

AGGTTCCTCCTTTCCTTAATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTT
AAACACCAAATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTC
CTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAA
ATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGG
AATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGgtgatgatgtaaagaataagaagcctgatccttcaatttatttagaagctgcaaagagattgggaaagtcagctaaaaattgtcttgttgtag
aggacagCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAGACTTCAGAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGA
ATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAATGATAGTACTTTCTTTTTCTCTTCGATTAGCAAAACCTGTTTTTTAAATGAATCAT
TCAGAAGATGCTTGC

Intron 16 GTCGGTATCT ... TCCTCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.95	8	2	8.7
A2	53.08	6.3	6	8.1
A3	31.14	12.6	7	4.4
A4	28.44	5.7	0	4.6
A5	55.33	3.3	6	2
A6	8.7	30.2	0	5.9
A7	29.35	26.7	12	10.3
A8	31.66	41.1	13	24.2
A9	10.12	34.3	5	2.6
A10	19.85	21.7	6	4.6

>TRINITY_DN41381_c0_g2_i7 from sample A6 / Example spliced isoform

TCAGTATATATGAGTCAGGCTTACTTCCTTTCCATTGCTTTCCATGACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGT
CTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGT
CATATCATACACCTTGTCAACAAAAGATCAAGACTTCAGAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGG
TCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAATGATAGTACTTTCTTTTTCTCTTCGATTAGCAAAG

>TRINITY_DN41381_c0_g2_i8 from sample A6 / Example unspliced isoform:

AATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCA
ATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCT
GCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCA
GAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGGAATTAAACTTGCAGTTTGTTCCG
CGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCTGGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTTGACATCTATAACATGACCAGACTAGA
GGTGGAATAGTTTAATTCAAACAGTCATGATTTTGGAAGGCCACCAATATTTTCTAAAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGCTTCTGAAATTGGGGATAAAACAAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGGTATTCGAATAAAAAGAAAAA
CATCATATATGCATGTCATTTATGATTTAACAGATCCAAGAAAGGTAACATGTTATCACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGAGCAATTCTAAATAACAAGCTACATTTCTGGGTGCCCTTGACATTGAAAGAAAAGCCTATCATCCAAATTTATACCCTGATTGAAATGCTTACTTGCTT
ACTTGTGAACTGCTGGATGTCATCAATAGTTGCAAGAATCCCATATTAGATAATTACTGTTGCCCCTTATTTTTAGCAATTTTTCTAACTGAATCCAAATTTGATGCTCCAACCTATGCTGGTAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGgtcggtatctttcaaaaac
aaacttactaggttttcataatgcaccaaacaaaaatttaagcacctcgtgcttttgggaatatattcatgcaatgttttccgtcatttcaatacttgagttttcttgatgttgttcctctacagAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCA
GGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAG

Intron 17 GTACTGCATC ... TCTTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.5	7.2	4.2	3.1
A2	44.05	7.2	4.8	0.5
A3	28.87	13.1	5.1	1.6
A4	70.87	4.4	10.2	4.5
A5	95.38	0.2	4	0.5
A6	15.09	15.1	2.5	1.1
A7	24.33	29.3	3.9	0
A8	20.3	38.5	3.2	0
A9	31.6	32	11.8	1.6
A10	22.07	23.8	4.8	0.5

>TRINITY_DN112951_c0_g1_i4 from sample A1 / Example spliced isoform

AAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAA
CACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTC
TATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAG
GCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTG
CAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAGACTTCAAAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAG
GACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGAC

>TRINITY_DN112951_c0_g1_i1 from sample A1 / Example unspliced isoform:

ATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGgtactgcatcccttgagtctatattctatta
ccgatgtttattggtttctctgatgttacagcctgcattcttttccgtaatagattttcctcttctgcagGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCTGGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTTGACATC
TATAACATGACCAGACTAGAGGTGGAATAGTTTAATTCAAACAGTCATGATTTTGGAAGGCCACCAATATTTTCTAAAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGCTTCTGAAATTGGGGATAAAACAAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGG
TATTCGAATAAAAAGAAAAACATCATATATGCATGTCATTTATGATTTAACAGATCCAAGAAAGGTAACATGTTATCACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGAGCAATTCTAAATAACAAGCTACATTTCTGGGTGCCCG

Intron 18 GTAAGTTTTA ... TTCCATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	5.93	11.1	3	0.6
A2	0	3.8	0	0
A3	18.34	10.4	0	2.5
A4	14.77	6.4	6	1.3
A5	19.48	8.5	2	2
A6	17.59	21	2	4.5
A7	14.64	39.4	2	6.3
A8	16.98	86.8	0	18.8
A9	17.95	51.5	3.4	11.4
A10	22.43	47.8	8	15.1

>TRINITY_DN43144_c0_g1_i4 from sample A7 / Example spliced isoform

CTTTCCTTAATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAA
ATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGG
CGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGG
TACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACT
TGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCG
TTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAGACTTCAGAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACT
TCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAATGATAGTACTTTCTTTTTCTCTTCGATTAGCAAAACC

>TRINITY_DN43144_c0_g1_i2 from sample A7 / Example unspliced isoform:

CAAGCTACATTTCTGGGTGCCCTTGACATTGAAAGAAAAGCCTATCATCCAAATTTATACCCTGATTGAAATGCTTACTTGCTTACTTGTGAACTGCTGGATGTCATCAATAGTTGCAAGAATCCCATATTAGATAATTACTGTTGCCCCTTATTTTTAGCAATTTTTCTAACTGAATCCAAATTTGATGCTCCAACCTA
TGCTGGTAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGGTCGGTATCTTTCAAAAACAAACTTACTAGGTTTTCATAATGCACCAAACAAAAATTTAAGCACCTCGTGCTTTTGGGAATATATTCATGCAATGTTTTCCGTCATTTCAATACTTGAGTTTTCTTGATGTTGTT
CCTCTACAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGTATGAAATAAAATTTTAGCCATGACAATCTCCTCCAAAAAATATTTGTAATCCTTTATTTTATATTTGCACCATTATGCTGCTGCTTATATTGGGGTATTATAAGGTTGTTACCTTCATAGTTTGATGGG
AAATCTGTGCATCTTTATTGATCAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAGTGTGTTCAATTATTATTGCGTTCTCCTCTTTAAGTCATATTTATGAAGCTGCAGAAAGAAAAATCAGATATTAACATCAAAGGCCTGAGTATGGTTTAAACTTTTTATTGTGTA
GGACTTCAGAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACAgtaagttttacactattaaaatcatcttgacttgtgtgagagtgggtgtgtgttttaccatttttagattttgattgggataaattttagctatcatgaacttgttaaggcctaattttatttaccaatttctgcacattgatttcactatc
tgtttccatgcagCTTGAAAGACCTTGACGCAC

Intron 19 GTCTGTTTTC ... TAGAGGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.73	3	3.5	8
A2	76.19	5	2	16
A3	61.11	7	3	11
A4	66.67	3	1	6
A5	66.67	1	1	2
A6	58.62	12	5	17
A7	56.25	21	0	27
A8	46.05	41	1.5	35
A9	51.61	30	6	32
A10	45	22	4	18

>TRINITY_DN38637_c0_g1_i5 from sample A9 / Example spliced isoform

TTTGTATCTGAGTGCCTGTATTTATTTCTATGGAATATACATTCGTTAGTGTCATCCAAAGGATTTTGTATGCCATCCGAGTATTGTCTCAATCATTAAAAGTTGTATTATACGATCAGTGGTAGCAACATAGAAGATTAGCATCTTGTTTGACATACGCAATGTTTTTCCACTAGCACAAACCTATTTAAACCTGGGCC
TGATATGCACCTCAAGGTAGAGAAGCTTGAGGTTGCATACTTGAATTCGAAGTTATTTGTGTGAACTTCAGATATGCTTTATGGATCTTAGCAGTTCAATGACCCTATCAAACCCAGGTTTATATGGGTCTGCACTGCTCTCTATACTCAAATGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGT
AAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTG
TGTCATATCATACACCTTGTCAACAAAAGATCAAGACTTCAAAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATT
TGGTCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAATGATAGTACTTTCTTTTTCTCTTCGATTAGCAAAACCTGTTTTTTAAATGAATCATTCAGAAGATGCTTGCAGTAGAACACATTGTGTAGTATACGATACACCCT
AAGCATCACATGTACAAAGTGAACATAACATACACATTTCTTTAA

>TRINITY_DN38637_c0_g1_i9 from sample A9 / Example unspliced isoform:

CCTTTCCTTAATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCA
AATTCTTCAATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAG
GCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTG
GTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGTATGATAAC
TAGCTTATTATTCCCTCACAATCTTCTCTGGACCAAGGCAGTAATCTGCCTAATAATAAAAAATTCCCCATAATGTAGAGATACATTAGTCAGGCAGTTAAGAATTACATTTCCAAATTGGTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGG
CCTGAATCAATTGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGTACTGCATCCCTTGAGTCTATATTCTATTACCGATGTTTATTGGTTTCTCTGATGTTACAGCCTGCATTC
TTTTCCGTAATAGATTTTCCTCTTCTGCAGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGgtctgttttcagcaatctggttctgtatcttgaattgtatcttagtattctttggttcctgattgaattgtcgaacttattgtgcactttgacatctataacatgaccagactagaggtggaatagtttaattcaa
acagtcatgattttggaaggccaccaatattttctaaaatctctacaagacaataatccaatcgatttaataattgtaatgcttctgaaattggggataaaacaaaggactttgatccctgtgagagatcttgtgttcaaaaagacccctattgttctggtattcgaataaaaagaaaaacatcatatatgcatgtcatt
tatgatttaacagatccaagaaaggtaacatgttatcactgctaaaaaacaacaataacatacagcaactgaaaagtagggagcaattctaaataacaagctacatttctgggtgcccttgacattgaaagaaaagcctatcatccaaatttataccctgattgaaatgcttacttgcttacttgtgaactgctggatgt
catcaatagttgcaagaatcccatattagataattactgttgcccctttttttagcaatttttctaactgaatccaaatttgatgctccaacctatgctggtaggtgatgatgtaaagaataagaagcctgatccttcaatttatttagaagctgcaaaggtcggtatctttcaaaaacaaacttactaggttttcataa
tgcaccaaacaaaaatttaagcacctcgtgcttttgggaatatattcatgcaatgttttccgtcatttcaatacttgagttttcttgatgttgttcctctacagagattgggaaagtcagctaaaaattgtcttgttgtagaggacagCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCA
TACACCTTGTCAACAAAAGATCAAGACTTCAGAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTA
CACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAATGATAGTACTTTCTTTTTCTCTTCGATTAGCAAAACCTGTTTTTTAAATGAATCATTCAGAAGATGCTTGCAGTAGAACACATTGTGTAGTATACGATACACCCTAAGCATCACA
TGTACAAAGTGAACATAACATACACATTTCTTTAA

Intron 20 GTCTGTTTTC ... ATGCTGGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50.69	4.7	3	6
A2	36.78	6.7	2	4
A3	49.91	5.7	3	8
A4	86.67	0.3	1	2
A5	66.67	2	1	5
A6	24.45	13	7	1
A7	21.77	22.3	0	9
A8	15.09	36.7	2	9
A9	23.25	31.3	11	6
A10	14.92	22	5	2

>TRINITY_DN41381_c0_g2_i7 from sample A6 / Example spliced isoform

TCAGTATATATGAGTCAGGCTTACTTCCTTTCCATTGCTTTCCATGACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGT
CTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGT
CATATCATACACCTTGTCAACAAAAGATCAAGACTTCAGAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGG
TCTCTTACACCTTATGGCATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAATGATAGTACTTTCTTTTTCTCTTCGATTAGCAAAG

>TRINITY_DN41381_c0_g2_i8 from sample A6 / Example unspliced isoform:

AATGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCA
ATCCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCT
GCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTTGGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCA
GAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGGAGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGGAATTAAACTTGCAGTTTGTTCCG
CGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGGAGCGTTTTCAACAGCTAGATTGCTTCCTTGCAGgtctgttttcagcaatctggttctgtatcttgaattgtatcttagtattctttggttcctgattgaattgtcgaacttattgtgcactttgacatctataacatgaccagactaga
ggtggaatagtttaattcaaacagtcatgattttggaaggccaccaatattttctaaaatctctacaagacaataatccaatcgatttaataattgtaatgcttctgaaattggggataaaacaaaggactttgatccctgtgagagatcttgtgttcaaaaagacccctattgttctggtattcgaataaaaagaaaaa
catcatatatgcatgtcatttatgatttaacagatccaagaaaggtaacatgttatcactgctaaaaaacaacaataacatacagcaactgaaaagtagggagcaattctaaataacaagctacatttctgggtgcccttgacattgaaagaaaagcctatcatccaaatttataccctgattgaaatgcttacttgctt
acttgtgaactgctggatgtcatcaatagttgcaagaatcccatattagataattactgttgccccttatttttagcaatttttctaactgaatccaaatttgatgctccaacctatgctggtagGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGGTCGGTATCTTTCAAAAAC
AAACTTACTAGGTTTTCATAATGCACCAAACAAAAATTTAAGCACCTCGTGCTTTTGGGAATATATTCATGCAATGTTTTCCGTCATTTCAATACTTGAGTTTTCTTGATGTTGTTCCTCTACAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCA
GGAATGGCTTGTGTCATATCATACACCTTGTCAACAAAAGATCAAG

Intron 21 GTACTGCATC ... TTTTCAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40	6	4	4
A2	33.33	6	5	1
A3	22.22	14	5	3
A4	52.38	5	10	1
A5	100	0	4	1
A6	13.51	16	2	3
A7	4.62	31	3	0
A8	4.76	30	3	0
A9	22.39	26	12	3
A10	10.34	26	4	2

>TRINITY_DN43427_c0_g1_i4 from sample A10 / Example spliced isoform

TGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCAAT
CCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTT
GGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGG
AGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGGAATTAAACTTGCAGTTTGT
TCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGCTAGATTGCTTCCTTGCAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAG

>TRINITY_DN43427_c0_g1_i2 from sample A10 / Example unspliced isoform:

TGTGGGGTCACACAAAAGAGATGAGGGATGGAACGTGAGAAGTTCAGTCATACAATAAATCATTGTTGGTTTTGGACCTTATCTTCATTACCCACATACCATGTAACAGAAACAAGAATACCTACATACCATGTAACAGAAACAACACAGAAGAAATGGGTTTCCTTCCTATGCTGAGCTTAAACACCAAATTCTTCAAT
CCTACTCACACCATTCATTCATATCCTTCCACGCAAATCTCAATTACAGTCGGCCAGCTGAATTCATGTCTTCCCCCACAACATCAACAGAACCAACTTCCTAACACCATAACCATTTCAAACCATGGGAAATTAAAAACACGGATTATTTCTGCTTCAGCAGCAGCAGCAGCATCTTCTCCTTCAAAGGCGACGGCGTT
GGAAACTCTCATTTTCGACTGTGATGGTGTGATTCTGGAATCTGAACATCTCCACAGAAATGCATATAATGCAACGTTCACCCACTTCAATGTCTGCTGTCCTTCTTCATCAAAACCGGTGGATTGGGATGCAGAATTCTATGATCAACTGCAGAACCAGATTGGTGGTGGGAAGCCCAAAATGCGCTGGTACTTTAAGG
AGAATGGTTGGCCTTCTTCAACTATCTTTCAAACACCTCCAGCTAATGAGACTGATAAAATCAAACTCATTGATAGTTTACAGGAATGGAAAACAGAGAAATACAAAGATATCATCCGTTCGGGGGCAGTAAAGCCAAGGCCAGGGGTATTGCAGTTAATGGATGAAGCCAGGGCAGCTGTATGATAACTAGCTTATTAT
TCCCTCACAATCTTCTCTGGACCAAGGCAGTAATCTGCCTAATAATAAAAAATTCCCCATAATGTAGAGATACATTAGTCAGGCAGTTAAGAATTACATTTCCAAATTGGTTATAACAAGCGTTACATTCACATGCGATCTAATCCTATATGGCAACAGTAGACAGTGTCCACGTCTCAGAAACCCAGGCCTGAATCAAT
TGGCAAGCACGGCTTAATACAATCTCTTTGTTCTGTGATGTAGGGAATTAAACTTGCAGTTTGTTCCGCGGCTACAAAAAGCTCAGTAATACTCTGTCTTGAAAATCTACTTGGGCTGgtactgcatcccttgagtctatattctattaccgatgtttattggtttctctgatgttacagcctgcattcttttccgtaat
agattttcctcttctgcaggagcgttttcaacagCTAGATTGCTTCCTTGCAGGTCTGTTTTCAGCAATCTGGTTCTGTATCTTGAATTGTATCTTAGTATTCTTTGGTTCCTGATTGAATTGTCGAACTTATTGTGCACTTTGACATCTATAACATGACCAGACTAGAGGTGGAATAGTTTAATTCAAACAGTCATGAT
TTTGGAAGGCCACCAATATTTTCTAAAATCTCTACAAGACAATAATCCAATCGATTTAATAATTGTAATGCTTCTGAAATTGGGGATAAAACAAAGGACTTTGATCCCTGTGAGAGATCTTGTGTTCAAAAAGACCCCTATTGTTCTGGTATTCGAATAAAAAGAAAAACATCATATATGCATGTCATTTATGATTTAAC
AGATCCAAGAAAGGTAACATGTTATCACTGCTAAAAAACAACAATAACATACAGCAACTGAAAAGTAGGGAGCAATTCTAAATAACAAGCTACATTTCTGGGTGCCCTTGACATTGAAAGAAAAGCCTATCATCCAAATTTATACCCTGATTGAAATGCTTACTTGCTTACTTGTGAACTGCTGGATGTCATCAATAGTT
GCAAGAATCCCATATTAGATAATTACTGTTGCCCCTTTTTTTAGCAATTTTTCTAACTGAATCCAAATTTGATGCTCCAACCTATGCTGGTAGGTGATGATGTAAAGAATAAGAAGCCTGATCCTTCAATTTATTTAGAAGCTGCAAAGGTCGGTATCTTTCAAAAACAAACTTACTAGGTTTTCATAATGCACCAAACA
AAAATTTAAGCACCTCGTGCTTTTGGGAATATATTCATGCAATGTTTTCCGTCATTTCAATACTTGAGTTTTCTTGATGTTGTTCCTCTACAGAGATTGGGAAAGTCAGCTAAAAATTGTCTTGTTGTAGAGGACAGCGTTATTGGTTTACAGGCAGCAATAGGGGCAGGAATGGCTTGTGTCATATCATACACCTTGTC
AACAAAAGATCAAGACTTCAGAGGTGCAAAAGCAATTTATCCGGACTTGAGTAATGTACACTTGAAAGACCTTGACGCACTACTTGAGGACAACCTGGTTGTTTCATAAGAAGAAAAGGATGAAGATGGAATAATCACTTCCAATATCTGGCATTCCTTGGCATCTAATTTTGTCAATTTGGTCTCTTACACCTTATGGC
ATTCACAGCAATTGTTGTTTCAGCAGAGAAATAGACTTGATTTTCAATTTGGACAAATCAATATGTTGTGTCAA