NA ----> NA

Intron 1 GTATGTACAG ... ACAGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.42	19	0	14
A2	57.45	20	8	27
A3	40.34	35.5	3	24
A4	43.48	13	3	10
A5	48.15	14	0	13
A6	46.09	138	9	118
A7	47.16	190.5	8	170
A8	45.69	145	5	122
A9	46.33	142.5	13	123
A10	44.95	98	10	80

>TRINITY_DN42489_c2_g1_i4 from sample A9 / Example spliced isoform

TGGGAATGAATAGACTACTAAGCGTCTCCCCTTTGCTCTCCTGCTCCGGCATTATAAGACCTTCACTCTCGAGAGTTCGGTCCTTTCGAAGAATCGGGATTTGTAAGGCGATGGCGGGAATCGATGGCGCGACCTCCCATGGGGCGGATGGTCCCTCCCCTTCTTCGGCCATTGATTTTCTCACTCTCTGCCAGCGCCTC
AAGACTACAAAACGAACTGGTTGGATTTACCGTGATATAAGGGATCCTGAATCTATTGCTGATCACATGTACCGGATGGCTGCTATGGCTCTTATTGCTGTGGATATTCCTGGTATCAATAGAGACAAGTGCATAAGGATGGCAATTGTCCATGACATTGCTGAAGCCATTGTTGGTGATATTACCCCTTCAGATGGTGT
CCCCAAGGAAGAAAAAAGCCGGAGAGAGAGGAAAGCTTTGGATGAAATGTGCGGAATACTGGGTGGAGGATTGAGAGCTGATGAAATACACCAGTTATGGAATGAGTATGAAGAGAATTCTTCTCCAGAAGCAAAAATTGTGAAAGATTTGGACAAGGTGGAAATGATTCTTCAAGCATTGGAGTATGAAACCGAGTCGA
GCCATTGAGAAGAGCCAAGAGCCTGTGTAGACACCGCATGAAGAGGCCGACTAGTCTACGTCGTGTGAAGAAGTAATATTGTTGATTCAAGGTTGCAGAAGAGCCATAGGGAGGAGCCGAAGGAAAGAGTTACCCATTGAAGTGTTGAGGGCACGCAGAATCAAAGCATTGAGGGCACACTAGTAGATTACCACCATCAA
TGTTGAGGGCACGTAGAATCGAAATATCGAGGGCACGCTAACATACCACCTCTAAGAATACTCAAACAAACTATAAAGAAAGGGCATGCAGACTATAAAGAAACACAAAACTGAAAATTACCACCTCCATAATTACTCCACGGCGCATGCAGGCTATAAAGAAATGTTGAAGTATTTAAAAAAATGGCCCCTGTCTGTTG
ATTTGAAGGAAGAATTATTTGAAAAGGGTTGCCGTCTGCTGATTGAAGGAAAGTCGAAATCCATCTTTTGCTCTAGTCTGTTTGTCGATTTGAAGGAAAGTCAAAGTCCATTTTTGCTCCAGTATTATCTGCCTATGTGAAGGAAAGTCGAGGTACCTTCATTTTTGCTACAGTATTTAAAATTCATGTTCAAAGATTTG
AAGATTTTCAACAGTCTAATTCGTTGATTTAGGACGACATAAGGGATTCTGGAAGTCATAAGCAACTACAGATTTAAAGCATTTTCAAAGACACAGAGCTTATATCTGTTCAGTCTCATGTCAGGACTGAGCCTCATGATTTCAGGTCGATGACTGGGTTAGCCATTCTTCTATCATACAAAGTAGATATGATGACAATG

CCTATGCCCAAGGCAAGAATTTAGATGAGTTTTTCCAGTCAACAGCAGGAAAGTTCCAAACTGATATTGGTAAAGCCTGGGCTGCTGAGATAGTTTCTAGGAGAAACAGAAGCTAACAACATGTGTGTAAATGATGTTATTCATCTAAGAGAACTGAACTGTTTGGCCTGGCAAACCGGTCTATCCATCTTCCAAGGTCA
TTCTTTATTTTCATAACCTCAAATAATTGTAAAGGGGCTTATTTGAGGTCATAGATGTCTGCAACATCTCTTAACATGTTATTGTACCAGTTAGTCTGGATGAAAATAAAATCACTTCATTTTATAGAAGAAAGGAAATTTGCATTCTGTGAGTCTAGAGGAATGTTGGAAATGAGGTGCCATCTTTTTTCTTCCTCTGG
TAATGGAGCCAATTTTAATCGGGTTACAAGCTGTGGGTGGAAAGACCTTGTATGATGTTTTTCCTCTCTTAGAGATCTTATGTTATATGGCAATTAGCATTGTTCCCATGACACATTGAAAATAATGAAATTTGGTTTATGACTAGAAAA

>TRINITY_DN42489_c2_g1_i5 from sample A9 / Example unspliced isoform:

TGGGAATGAATAGACTACTAAGCGTCTCCCCTTTGCTCTCCTGCTCCGGCATTATAAGACCTTCACTCTCGAGAGTTCGGTCCTTTCGAAGAATCGGGATTTGTAAGGCGATGGCGGGAATCGATGGCGCGACCTCCCATGGGGCGGATGGTCCCTCCCCTTCTTCGGCCATTGATTTTCTCACTCTCTGCCAGCGCCTC
AAGACTACAAAACGAACTGGTTGGATTTACCGTGATATAAGGGATCCTGAATCTATTGCTGATCACATGTACCGGATGGCTGCTATGGCTCTTATTGCTGTGGATATTCCTGGTATCAATAGAGACAAGTGCATAAGGATGGCAATTGTCCATGACATTGCTGAAGCCATTGTTGGTGATATTACCCCTTCAGATGGTGT
CCCCAAGGAAGAAAAAAGCCGGAGAGAGAGGAAAGCTTTGGATGAAATGTGCGGAATACTGGGTGGAGGATTGAGAGCTGATGAAATACACCAGTTATGGAATGAGTATGAAGAGAATTCTTCTCCAGAAGCAAAAATTGTGAAAGATTTGGACAAGGTGGAAATGATTCTTCAAGCATTGGAGTATGAAACCGCAGACA
CAGAGCTTATATCTGTTCAGTCTCATGTCAGGACTGAGCCTCATGATTTCAGGTCGATGACTGGGTTAGCCATTCTTCTATCATACAAAGTAGATATGATGACAATGCCTATGgtatgtacagtagcaatgttccagtgtcattcagtggttacatattacagatgcagCCCAAGGCAAGAATTTAGATGAGTTTTTCCA
GTCAACAGCAGGAAAGTTCCAAACTGATATTGGTAAAGCCTGGGCTGCTGAGATAGTTTCTAGGAGAAACAGAAGCTAACAACATGTGTGTAAATGATGTTATTCATCTAAGAGAACTGAACTGTTTGGCCTGGCAAACCGGTCTATCCATCTTCCAAGGTCATTCTTTATTTTCATAACCTCAAATAATTGTAAAGGGG
CTTATTTGAGGTCATAGATGTCTGCAACATCTCTTAACATGTTATTGTACCAGTTAGTCTGGATGAAAATAAAATCACTTCATTTTATAGAAGAAAGGAAATTTGCATTCTGTGAGTCTAGAGGAATGTTGGAAATGAGGTGCCATCTTTTTTCTTCCTCTGGTAATGGAGCCAATTTTAATCGGGTTACAAGCTGTGGG
TGGAAAGACCTTGTATGATGTTTTTCCTCTCTTAGAGATCTTATGTTATATGGCAATTAGCATTGTTCCCATGACACATTGAAAATAATGAAATTTGGTTTATGACTAGAAAA

Intron 2 GTTGTTCAAA ... GTGCTAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.73	50.5	15	16
A2	22.16	45	19	6
A3	23.62	53	14	17
A4	23.96	20	6	6
A5	25.21	33	10	11
A6	26.09	373	132	110
A7	22.55	456	114	134
A8	26	400	129	132
A9	26.53	404.5	122	152
A10	25.59	250	90	66

>TRINITY_DN42614_c2_g1_i3 from sample A6 / Example spliced isoform

AGAATCTGATTGAATTGGAGATAGTTGATCGAATAGATAGCAAAAATTAAACGCATATACCATTGCACGGAGGGAATGGGAATGAATAGACTACTAAGCGTCTCCCCTTTGCTCTCCTGCTCCGGCATTATAAGACCTTCACTCTCGAGAGTTCGGTCCTTTCGAAGAATCGGGATTTGTAAGGCGATGGCGGGAATCGA
TGGCGCGACCTCCCATGGGGCGGATGGTCCCTCCCCTTCTTCGGCCATTGATTTTCTCACTCTCTGCCAGCGCCTCAAGACTACAAAACGAACTGGTTGGATTTACCGTGATATAAGGGATCCTGAATCTATTGCTGATCACATGTACCGGATGGCTGCTATGGCTCTTATTGCTGTGGATATTCCTGGTATCAATAGAG
ACAAGTGCATAAGGATGGCAATTGTCCATGACATTGCTGAAGCCATTGTTGGTGATATTACCCCTTCAGATGGTGTCCCCAAGGAAGAAAAAAGCCGGAGAGAGAGGAAAGCTTTGGATGAAATGTGCGGAATACTGGGTGGAGGATTGAGAGCTGATGAAATACACCAGTTATGGAATGAGTATGAAGAGAATTCTTCT
CCAGAAGCAAAAATTGTGAAAGATTTGGACAAGGTGGAAATGATTCTTCAAGCATTGGAGTATGAAACCGCAGACACAGAGCTTATATCTGTTCAGTCTCATGTCAGGACTGAGCCTCATGATTTCAGGTCGATGACTGGGTTAGCCATTCTTCTATCATACAAAGTAGATATGATGACAATGCCTATGGTATGTACAGT
AGCAATGTTCCAGTGTCATTCAGTGGTTACATATTACAGATGCAGCCCAAGGCAAGAATTTAGATGAGTTTTTCCAGTCAACAGCAGGAAAGTTCCAAACTGATATTGGTAAAGCCTGGGCTGCTGAGATAGTTTCTAGGAGAAACAGAAGCTAACAACATGTGTGTAAATGATGTTATTCATCTAAGAGAACTGAACTG
TTTGGCCTGGCAAACCGGTCTATCCATCTTCCAAGGTCATTCTTTATTTTCATAACCTCAAATAATTGTAAAGGGGCTTATTTGAGGTCATAGATGTCTGCAACATCTCTTAACATGTTATTGTACCAGTTAGTCTGGATGAAAATAAAATCACTTCATTTTATAGAAGAAAGGAAATTTGCATTCTGTGAGTCTAGAGG
AATGTTGGAAATGAGGTGCCATCTTTTTTCTTCCTCTGGTAATGGAGCCAATTTTAATCGGGTTACAAGCTGTGGGTGGAAAGACCTTGTATGATGTTTTTCCTCTCTTAGAGATCTTATGTTATATGGCAATTAGCATTGTTCCCATGACACATTGAAAATAATGAAATTTGGTTTATGACTAAAAAAA

>TRINITY_DN42614_c2_g1_i2 from sample A6 / Example unspliced isoform:

AGAATCTGATTGAATTGGAGATAGTTGATCGAATAGATAGCAAAAATTAAACGCATATACCATTGCACGGAGGGAATGGGAATGAATAGACTACTAAGCGTCTCCCCTTTGCTCTCCTGCTCCGGCATTATAAGACCTTCACTCTCGAGAGTTCGGTCCTTTCGAAGAATCGGGATTTGTAAGGCGATGGCGGGAATCGA
TGGCGCGACCTCCCATGGGGCGGATGGTCCCTCCCCTTCTTCGGCCATTGATTTTCTCACTCTCTGCCAGCGCCTCAAGACTACAAAACGAACTGGTTGGATTTACCGTGATATAAGGGATCCTGAATCTATTGCTGATCACATGTACCGGATGGCTGCTATGGCTCTTATTGCTGTGGATATTCCTGGTATCAATAGAG
ACAAGTGCATAAGGATGGCAATTGTCCATGACATTGCTGAAGCCATTGTTGGTGATATTACCCCTTCAGATGGTGTCCCCAAGGAAGAAAAAAGCCGGAGAGAGAGGAAAGCTTTGGATGAAATGTGCGGAATACTGGGTGGAGGATTGAGAGCTGATGAAATACACCAGTTATGGAATGAGTATGAAGAGAATTCTTCT
CCAGAAGCAAAAATTGTGAAAGATTTGGACAAGGTGGAAATGATTCTTCAAGCATTGGAGTATGAAACCGCCCAAGGCAAGAATTTAGATGAGTTTTTCCAGTCAACAGCAGGgttgttcaaacttcagacatgacgagtcaccagtgctaaaagAAAGTTCCAAACTGATATTGGTAAAGCCTGGGCTGCTGAGATAGT
TTCTAGGAGAAACAGAAGCTAACAACATGTGTGTAAATGATGTTATTCATCTAAGAGAACTGAACTGTTTGGCCTGGCAAACCGGTCTATCCATCTTCCAAGGTCATTCTTTATTTTCATAACCTCAAATAATTGTAAAGGGGCTTATTTGAGGTCATAGATGTCTGCAACATCTCTTAACATGTTATTGTACCAGTTAG
TCTGGATGAAAATAAAATCACTTCATTTTATAGAAGAAAGGAAATTTGCATTCTGTGAGTCTAGAGGAATGTTGGAAATGAGGTGCCATCTTTTTTCTTCCTCTGGTAATGGAGCCAATTTTAATCGGGTTACAAGCTGTGGGTGGAAAGACCTTGTATGATGTTTTTCCTCTCTTAGAGATCTTATGTTATATGGCAAT
TAGCATTGTTCCCATGACACATTGAAAATAATGAAATTTGGTTTATGACTAAAAAAA

Intron 3 GTGCATAAGG ... ATTGCTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76	24	72	80
A2	78.57	18	63	69
A3	78.64	22	82	80
A4	78.31	18	63	67
A5	62.89	18	25	36
A6	76.79	110	366	362
A7	78.12	113	390	417
A8	74.92	114	330	351
A9	74.14	139	379	418
A10	79.89	73	296	284

>TRINITY_DN96678_c0_g1_i3 from sample A5 / Example spliced isoform

GGGAATGAATAGACTACTAAGCGTCTCCCCTTTGCTCTCCTGCTCCGGCATTATAAGACCTTCACTCTCGAGAGTTCGGTCCTTTCGAAGAATCGGGATTTGTAAGGCGATGGCGGGAATCGATGGCGCGACCTCCCATGGGGCGGATGGTCCCTCCCCTTCTTCGGCCATTGATTTTCTCACTCTCTGCCAGCGCCTCA
AGACTACAAAACGAACTGGTTGGATTTACCGTGATATAAGGGATCCTGAATCTATTGCTGATCACATGTACCGGATGGCTGCTATGGCTCTTATTGCTGTGGATATTCCTGGTATCAATAGAGACAACCATTGTTGGTGATATTACCCCTTCAGATGGTGTCCCCAAGGAAGAAAAAAGCCGGAGAGAGAGGAAAGCTTT
GGATGAAATGTGCGGAATACTGGGTGGAGGATTGAGAGCTGATGAAATACACCAGTTATGGAATGAGTATGAAGAGAATTCTTCTCCAGAAGCAAAAATTGTGAAAGATTTGGACAAGGTGGAAATGATTCTTCAAGCATTGGAGTATGAAACCGCAGACACAGAGCTTATATCTGTTCAGTCTCATGTCAGGACTGAGC
CTCATGATTTCAGGTCGATGACCGGG

>TRINITY_DN96678_c0_g1_i2 from sample A5 / Example unspliced isoform:

GGGAATGAATAGACTACTAAGCGTCTCCCCTTTGCTCTCCTGCTCCGGCATTATAAGACCTTCACTCTCGAGAGTTCGGTCCTTTCGAAGAATCGGGATTTGTAAGGCGATGGCGGGAATCGATGGCGCGACCTCCCATGGGGCGGATGGTCCCTCCCCTTCTTCGGCCATTGATTTTCTCACTCTCTGCCAGCGCCTCA
AGACTACAAAACGAACTGGTTGGATTTACCGTGATATAAGGGATCCTGAATCTATTGCTGATCACATGTACCGGATGGCTGCTATGGCTCTTATTGCTGTGGATATTCCTGGTATCAATAGAGACAAgtgcataaggatggcaattgtccatgacattgctgaagCCATTGTTGGTGATATTACCCCTTCAGATGGTGTC
CCCAAGGAAGAAAAAAGCCGGAGAGAGAGGAAAGCTTTGGATGAAATGTGCGGAATACTGGGTGGAGGATTGAGAGCTGATGAAATACACCAGTTATGGAATGAGTATGAAGAGAATTCTTCTCCAGAAGCAAAAATTGTGAAAGATTTGGACAAGGTGGAAATGATTCTTCAAGCATTGGAGTATGAAACCGCAGACAC
AGAGCTTATATCTGTTCAGTCTCATGTCAGGACTGAGCCTCATGATTTCAGGTCGATGACCGGG