NA ----> NA

Intron 1 GTAGGTTTCA ... TAGTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.79	132.7	23	4
A2	15.96	121.3	23	2
A3	20.65	123	32	4
A4	10.24	96.7	11	3
A5	15.86	37.7	7	0
A6	12.49	238.7	34	0
A7	9.51	400.3	42	0
A8	12.3	285.7	40	10
A9	11.56	360.7	47	0
A10	15.43	362.7	66	2

>TRINITY_DN119480_c0_g1_i1 from sample A1 / Example spliced isoform

AATTATTGCCATTAATTACAGGTAAGCTTATTCTGTGTTTACTGTTGGTTCTAAAATGCCATTTGACATATATTTGCAGGCCTCTGCAGTTGCTAGAAGTGCAATTTCAGATTTGGAAAGTGCAGCTGAGCCATTGTCAGAGCCTGTTAAAGAAGATGAAACAGAGGCAGAGAGTGTAGCAACACAACCTGAAGATGATA
ATGAAGAGGAGAAACGACGGAAGGCTGCACTGGATCGACTGGAGAAAGCCAGCCAAGATACATTACTGGGGCAGGGACTCAGGGCTTTTGATAGTTCAGTGGAGAGTTTTGCATCTGGAGCCTGGCAAGCTTTTGGCAGTGCTTTGAAAGAAGGCACCAACTTGGTGCAGAAGATTGAGCATTCAGCTGTGAATCTTGCT
GATACTATTCAACAAGGTGGCCTAACCACTAAAGCTAGCTCCATTGCCCCAACTTTGATAGAGAGTGGAAAAGCCTTTACAGCCAAGGGAATGCAAGTGCTAGAGCTTGTTGGTAGAGAGACCATTGATCTGCTAGCTTCAGAGACAGGCATTGAGATTGAGAAAGATGGTAGGTTGACTGAACAAGGTGGGGGTGAGGA
ACAGTTCTCTGAAGAAGCAACTTTTGACCGATGCTTTTGGATTTATGGAGGTCCTGAGCAACTTGAGGAGCTGGAAGCTTTGTCCAATCATTATGCATTACTATGCAATAGAACAAAAGCCAAATTATCAGGAGAAGATAAAGCTGCCTTTGATGGAAGGCTCAAGCAGGTCCAACAGATCTTTACATTAAGTAATGAAA
GCGATGGAGGTAACTTGGATTTTAACAAGGGTAAGAATGTTGAGAGTGCAGAAATGGGCAATGGAAATGAAATAAAAACCCTTTGTGAATCCAGTGTCAACAAAGCAGCTGATATGGCAGCAGGATTTACAGCGGCTCTTGGTGGACTTGCTTTTGATGAGATAGTTCAGAAAACATCTGGTCGTCTTGAGGCAATTAGA
GCTGAAGCAGTGCATAGGCTTTCAGAGATGTGTTGTCTTGGGATATCTGATCTCCTTCTTCTTGGCAAATCTGTCCTGTCAACGAGTCCTAAGGCACAAAATGACAAAGTAATGGAAGATTCTTTGAAAATTGAATGGCCAGATGATTATATTTTGAAGGCAAAGTTTATCAGATCAAAAGCACAGGCTATGACTGATGA
TATTGAGGCTGTTTCAAACAGTTTTACCACTGGGATATCAGATATTATTGCTGCCTTTCAAGAAGCCATCAGGAATGCATCACAAAATGGACGAGAAGCCAGACAGGGAATTTTGCAAGAAAGCTCTATTGAAGACAAGGCACAAATTCTCACAAGTGACATAGAGACTGATGGAAGCACAGCCATTGAGAAAATTCAGG
ATGCACTTCAGCATTTAGTTTTTGTGGTGTTCTCTAGCACATTGAAGAGCTGAGGACTAACAATATGCTTACAGCCAGCTCAACTCAATATTTGAATTCAAATGATGTGCAGATTTCCTAAGAACCGTAATGATGTTGTATCTATGTCATTCTTAATTGTTTTTTTTACTTGAATTTTAAATTAGATGTATACATATTTA
GGTCTCTTGTTGAACTGACTCATTTGTACAAATGTATTTCATTTTGGTAAATAAAAGGAGATTTATTAGAGTAAAAA

>TRINITY_DN119480_c0_g1_i4 from sample A1 / Example unspliced isoform:

CCTCTGCAGTTGCTAGAAGTGCAATTTCAGATTTGGAAAGTGCAGCTGAGCCATTGTCAGAGCCTGTTAAAGAAGATGAAACAGAGGCAGAGAGTGTAGCAACACAACCTGAAGATGATAATGAAGAGGAGAAACGACGGAAGGCTGCACTGGATCGACTGGAGAAAGCCAGCCAAGATACATTACTGGGGCAGgtaggt
ttcagtcatattctccagttactgcttttatagatccagaggaatatatccggttattcttcagttactttttttctagatccagggtggttgaagtgcacagctttgcttgtaggtgtctgtgtgattcatttctgttagtttacagGGACTCAGGGCTTTTGATAGTTCAGTGGAGAGTTTTGCATCTGGAGCCTGGC
AAGCTTTTGGCAGTGCTTTGAAAGAAGGCACCAACTTGGTGCAGAAG

Intron 2 GTAAGGTGCT ... ATATCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	9.57	328	38.3	36.7
A2	8.16	278	27.2	25.7
A3	12.23	271	28.2	40
A4	8.97	216	39.2	22
A5	9.12	164	6.5	17.3
A6	8.6	586	38.2	57.7
A7	7.43	1108.2	95.2	92.7
A8	7.76	843	66.7	74
A9	8.27	1010	118.3	95.3
A10	7.86	1055	92.5	94

>TRINITY_DN110702_c0_g1_i1 from sample A3 / Example spliced isoform

GTCAGACACAGGAGGTGAACTTGTAAGTAATAAGAAACGATTGGCACCGGATCGGAGCAATCGAATGACGAAGACGGCCCGGATGGATGTCTGAACAGAACGAGTGAATCGAAGATCGAGCAGAAGCGAAATACCCAGGTCGACCGAATGGCGGATTCACATTCTCCCTCTTCAGATTCGACAGAGCCCAGAGGATGGGG
GACATGGCGCACATCTGCTTTCTCAGTGTTTTCTGATCTTCAAAAGGCAGCAGCTCAGGCAGCCGACGAAATTACTCGCAATGCCTCTGCAGTTGCTAGAAGTGCAATTTCAGATTTGGAAAGTGCAGCTGAGCCATTGTCAGAGCCTGTTAAAGAAGATGAAACAGAGGCAGAGAGTGTAGCAACACAACCTGAAGATG
ATAATGAAGAGGAGAAACGACGGAAGGCTGCACTGGATCGACTGGAGAAAGCCAGCCAAGATACATTACTGGGGCAGGGACTCAGGGCTTTTGATAGTTCAGTGGAGAGTTTTGCATCTGGAGCCTGGCAAGCTTTTGGCAGTGCTTTGAAAGAAGGCACCAACTTGGTGCAGAAGATTGAGCATTCAGCTGTGAATCTT
GCTGATACTATTCAACAAGGTGGCCTAACCACTAAAGCTAGCTCCATTGCCCCAACTTTGATAGAGAGTGGAAAAGCCTTTACAGCCAAGGGAATGCAAGTGCTAGAGCTTGTTGGTAGAGAGACCATTGATCTGCTAGCTTCAGAGACAGGCATTGAGATTGAGAAAGATGGTAGGTTGACTGAACAAGGTGGGGGTGA
GGAACAGTTCTCTGAAGAAGCAACTTTTGACCGATGCTTTTGGATTTATGGAGGTCCTGAGCAACTTGAGGAGCTGGAAGCTTTGTCCAATCATTATGCATTACTATGCAATAGAACAAAAGCCAAATTATCAGGAGAAGATAAAGCTGCCTTTGATGGAAGGCTCAAGCAGGTCCAACAGATCTTTACATTAAGTAATG
AAAGCGATGGAGGTAACTTGGATTTTAACAAGGGTAAGAATGTTGAGAGTGCAGAAATGGGCAATGGAAATGAAATAAAAACCCTTTGTGAATCCAGTGTCAACAAAGCAGCTGATATGGCAGCAGGATTTACAGCGGCTCTTGGTGGACTTGCTTTTGATGAGATAGTTCAGAAAACATCTGGTCGTCTTGAGGCAATT
AGAGCTGAAGCAGTGCATAGGCTTTCAGAGCTGTGTTGTCTTGGGATATCTGATCTCCTTCTTCTTGGCAAATCTGTCCTGTCAACGAGTCCTAAGGCACAAAATGACAAAGTAATGGAAGATTCTTTGAAAATTGAATGGCCAGATGATTATATTTTGAAGGCAAAGTTTATCAGATCAAAAGCACAGGCTATGACTGA
TGATATTGAGGCTGTTTCAAACAGTTTTACCACTGGGATATCAGATATTATTGCTGCCTTTCAAGAAGCCATCAGGAATGCATCACAAAATGGACGAGAAGCCAGACAGGGAATTTTGCAAGAAAGCTCTATTGAAGACAAGGCACAAATTCTCACAAGTGACATAGAGACTGATGGAAGCACAGCCATTGAGAAAATTC
AGGATGCACTTCAGCATTTAGTTTTTGTGGTGTTCTCTAGCACATTGAAGAGCTGAGGACTAACAATATGCTTACAGCCAGCTCAACTCAATATTTGAATTCAAATGATGTGCAGATTTCCTAAGAACCGTAATGATGTTGTATCTATGTCATTCTTAATTGTTTTTTTTACTTGAATTTTAAATTAGATGTATACATAT
TTAGGTCTCTTGTTGAACTGACTCATTTGTACAAATGTATTTCATTTTGGTAAATAAAAGGAGATTTATTAGAGTAAAAA

>TRINITY_DN110702_c0_g1_i4 from sample A3 / Example unspliced isoform:

GTCAGACACAGGAGGTGAACTTGTAAGTAATAAGAAACGATTGGCACCGGATCGGAGCAATCGAATGACGAAGACGGCCCGGATGGATGTCTGAACAGAACGAGTGAATCGAAGATCGAGCAGAAGCGAAATACCCAGGTCGACCGAATGGCGGATTCACATTCTCCCTCTTCAGATTCGACAGAGCCCAGAGGATGGGG
GACATGGCGCACATCTGCTTTCTCAGTGTTTTCTGATCTTCAAAAGGCAGCAGCTCAGGCAGCCGACGAAATTACTCGCAATGCCTCTGCAGTTGCTAGAAGTGCAATTTCAGATTTGGAAAGTGCAGCTGAGCCATTGTCAGAGCCTGTTAAAGAAGATGAAACAGAGGCAGAGAGTGTAGCAACACAACCTGAAGATG
ATAATGAAGAGGAGAAACGACGGAAGGCTGCACTGGATCGACTGGAGAAAGCCAGCCAAGATACATTACTGGGGCAGGGACTCAGGGCTTTTGATAGTTCAGTGGAGAGTTTTGCATCTGGAGCCTGGCAAGCTTTTGGCAGTGCTTTGAAAGAAGGCACCAACTTGGTGCAGAAGATTGAGCATTCAGCTGTGAATCTT
GCTGATACTATTCAACAAGGTGGCCTAACCACTAAAGCTAGCTCCATTGCCCCAACTTTGATAGAGAGTGGAAAAGCCTTTACAGCCAAGGGAATGCAAGTGCTAGAGCTTGTTGGTAGAGAGACCATTGATCTGCTAGCTTCAGAGACAGGCATTGAGATTGAGAAAGATGGTAGGTTGACTGAACAAGGTGGGGGTGA
GGAACAGTTCTCTGAAGAAGCAACTTTTGACCGATGCTTTTGGATTTATGGAGGTCCTGAGCAACTTGAGgtaaggtgctttttagtgtcctgttatctaaatgctatcaagtgtcatctgaaggactgaacaattttgatgtctgcagGAGCTGGAAGCTTTGTCCAAT

Intron 3 GTGAGAGCAT ... ATTTCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.25	172	0	41
A2	20.64	173	0	45
A3	20.71	157	0	41
A4	19.89	149	0	37
A5	18.87	86	0	20
A6	20.18	609	0	154
A7	17.39	798	0	168
A8	22.51	802	0	233
A9	22.19	712	0	203
A10	19.76	743	0	183

>TRINITY_DN103443_c0_g1_i8 from sample A4 / Example spliced isoform

GTCAGACACAGGAGGTGAACTTGTAAGTAATAAGAAACGATTGGCACCGGATCGGAGCAATCGAATGACGAAGACGGCCCGGATGGATGTCTGAACAGAACGAGTGAATCGAAGATCGAGCAGAAGCGAAATACCCAGGTCGACCGAATGGCGGATTCACATTCTCCCTCTTCAGATTCGACAGAGCCCAGAGGATGGGG
GACATGGCGCACATCTGCTTTCTCAGTGTTTTCTGATCTTCAAAAGGCAGCAGCTCAGGCAGCCGACGAAATTACTCGCAATGCCTCTGCAGTTGCTAGAAGTGCAATTTCAGATTTGGAAAGTGCAGCTGAGCCATTGTCAGAGCCTGTTAAAGAAGATGAAACAGAGGCAGAGAGTGTAGCAACACAACCTGAAGATG
ATAATGAAGAGGAGAAACGACGGAAGGCTGCACTGGAACGACTGGAGAAAGCCAGCCAAGATACATTACTGGGGCAGGGACTCAGGGCTTTTGATAGTTCAGTGGAGAGTTTTGCATCTGGAGCCTGGCAAGCTTTTGGCAGTGCTTTGAAAGAAGGCACCAACTTGGTGCAGAAGATTGAGCATTCAGCTGTGAATCTT
GCTGATACTATTCAACAAGGTGGCCTAACCACTAAAGCTAGCTCCATTGCCCCAACTTTGATAGAGAGTGGAAAAGCCTTTACAGCCAAGGGAATGCAAGTGCTAGAGCTTGTTGGTAGAGAGACCATTGATCTGCTAGCTTCAGAGACAGGCATTGAGATTGAGAAAGATGGTAGGTTGACTGAACAAGGTGGGGGTGA
GGAACAGTTCTCTGAAGAAGCAACTTTTGACCGATGCTTTTGGATTTATGGAGGTCCTGAGCAACTTGAGGAGCTGGAAGCTTTGTCCAATCATTATGCATTACTATGCAATAGAACAAAAGCCAAATTATCAGGAGAAGATAAAGCTGCCTTTGATGGAAGGCTCAAGCAGGTCCAACAGATCTTTACATTAAGTAATG
AAAGCGATGGAGGTAACTTGGATTTTAACAAGGGTAAGAATGTTGAGAGTGCAGAAATGGGCAATGGAAATGAAATAAAAACCCTTTGTGAATCCAGTGTCAACAAAGCAGCTGATATGGCAGCAGGATTTACAGCGGCTCTTGGTGGACTTGCTTTTGATGAGATAGTTCAGAAAACATCTGGTCGTCTTGAGGCAATT
AGAGCTGAAGCAGTGCATAGGCTTTCAGAGATGTGTTGTCTTGGGATATCTGATCTCCTTCTTCTTGGCAAATCTGTCCTGTCAACGAGTCCTAAGGCACAAAATGACAAAGTAATGGAAGATTCTTTGAAAATTGAATGGCCAGATGATTATATTTTGAAGGCAAAGTTTATCAGATCAAAAGCACAGGCTATGACTGA
TGATATTGAGGCTGTTTCAAACAGTTTTACCACTGGTATGTAGATATTACATTCCTGGAAACTTTTTACTATTATGAAACCTGACTGTCCACCATGCTAATTGTGTTCAGATGGAAAAAG

>TRINITY_DN103443_c0_g1_i2 from sample A4 / Example unspliced isoform:

CAATTAGAGCTGAAGCAGTGCATgtgagagcatcagcaaatccctagtaacccaagcttgtcataagatgcacatgttcaagcagaaatttatcctggattttgttacaattttaatggtactatttgatatctatatagaaaaccttataccatctctgaatgttttttatgcttcatttctacagAGGCTTTCAGAGA
TGTGTTGTCTTGGGATATCTGATCTCCTTCTTCTTGGCAAATCTGTCCTGTCAACGAGTCCTAAGGCACAAAATGACAAAGTAATGGAAGATTCTTTGAAAATTGAATGGCCAGATGATTATATTTTGAAGGCAAAGTTTATCAGATCAAAAGCACAGGCTATGACTGATGATATTGAGGCTGTTTCAAACAGTTTTACC
ACTGG