sp|Q76H85|H4_SILLA ----> Histone H4

Intron 1 GTTCTTACCT ... ACCTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.54	5	8	5
A2	52.38	10	11	6
A3	60	8	12	11
A4	42.86	12	9	10
A5	52.17	11	12	11
A6	63.06	41	70	20
A7	54.94	36.5	44.5	8
A8	68.67	26	57	6
A9	68.21	31	66.5	13
A10	57.86	33.5	46	13

>TRINITY_DN120707_c7_g1_i2 from sample A1 / Example spliced isoform

GGAACAGACAGAAAGAAATAGAACCATGTCTGGGCGTGGAAAGGGAGGCAAGGGTTTGGGTAAAGGAGGGGCCAAGAGGCACAGGAAGGTTTTGAGAGACAACATTCAGGGCATAACGAAGCCGGCCATACGGCGTCTTGCCAGGAGGGGAGGTGTCAAGCGAATCAGCGGCCTTATTTACGAAGAAACGAGGGGGGTTT
TGAAGATCTTCTTGGAACATGTTATCAAGGATGCCGTCACTTACACTGAGCACGCCCGAAGGAAGACCGTCACTGCCATGGATGTTGTATATTCCCTCAAGCGGCAGGGCAGGACTCTCTACGGATTCGGTGGTTGAATAACTTCCAATGGACCGCCCAAACTCTATTTGTTCAAATCCAATCTCTCGGTCCATTTATTT
CTCCTTTCATGGTGTTTCTGGACACCACCCGCTGCATCTCTGGAGGTATATCGAAGACTTACATTTACCCAGATGTCTTTGGAGCATTTGCATTTGCTATAGGTATATCGAAGACTTACATTTACCCAGATGTCTTTGGAGCATTTGCATTTGCTATAGGTATATCGAAGACTTACATTT

>TRINITY_DN120707_c7_g1_i1 from sample A1 / Example unspliced isoform:

GGAACAGACAGAAAGAAATAGAACCATGTCTGGGCGTGGAAAGGGAGGCAAGGGTTTGGGTAAAGGAGGGGCCAAGAGGCACAGGAAGGTTTTGAGAGACAACATTCAGGGCATAACGAAGCCGGCCATACGGCGTCTTGCCAGGAGGGGAGGTGTCAAGCGAATCAGCGGCCTTATTTACGAAGAAACGAGGGGGGTTT
TGAAGATCTTCTTGGAACATGTTATCAAGGATGCCGTCACTTACACTGAGCACGCCCGAAGGAAGACCGTCACTGCCATGGATGTTGTATATTCCCTCAAGCGGCAGGGCAGGACTCTCTACGGATTCGGTGGTTGAATAACTTCCAATGGACCGCCCAAACTCTATTTGTTCAAATCCAATCTCTCGGTCCATTTATTT
CTCCTTTCATGGTGTTTCTGGACACCACCCGCTGCATCTCTGGAGgttcttaccttttgctgtctgtcataaccagaagcaaaaatagaaaacacgggattaagatataaatgtattcgcaatgatgtgtgcctcgcaatttgttttggttttattgtcgtttgtattcagcaaacacttttgttttcaaatgtcaaaat
agtgtgtgaagttttgtccaggattagtaatcaggaaattgtttagggttagtgggaagggtaagaatcttgagggacttaaccatggcttatataggcctaaaattgcaatttttttcctttaatttagggacagctttgttaatttcgaaatttagcaatggttttggtgtaagtttacaataacatattcctggtca
atgtttaagtgtatttattttgaaatttggcaatggttgtgcacctgtacagGTATATCGAAGACTTACATTTACCCAGATGTCTTTGGAGCATTTGCATTTGCTATAGGTATATCGAAGACTTACATTTACCCAGATGTCTTTGGAGCATTTGCATTTGCTATAGGTATATCGAAGACTTACATTTAGAGCATTTGCTA
ATCCCTGAGTTAGTGATTAACAGTTATAATTAGATTTAAGAAACGAACAACATAGTATCAGTCTGCAGCATATGAAGATAACATGGGGATGTTTATAATGTAAGTGCGTTACGTGATTTTGTTAGTTTCAATTCGCGGTTGACAATGATGATGGAAATGATTAAAGAAATTGGTATCAGGGTTTCTGAGCAGTGACTTCT
GTTTCCTCTGTTGTAACCACATCGATGTTCTCTCCTTTGTTGCCCAATTCCTTGTTTTTGTCATTGTTTTCCCCATTGTTCCTTATTTTCTAGTCCGAATATTTTTAAAGTATTTTTTTTTTAAACTATGGGAATAGCAATCTCTCAAGGGTATATCTAAGAGTCTGTAAGTCAGATATACCAGAAATTTGTGATATCCC
GAGACTCTTTATCCCAAAGAAGAGCCTCTATCTCAG

Intron 2 GTTCTTACCT ... ACCTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.21	3	8	5
A2	55.13	9	11	6
A3	60	8	12	11
A4	47.9	10	9	10
A5	56.58	9.5	12	12
A6	67.25	34.5	70	22
A7	58.7	31.9	44.9	8
A8	71.35	23	57	5
A9	69.59	29	66.1	13
A10	61.7	28.9	46	13

>TRINITY_DN42792_c0_g1_i3 from sample A6 / Example spliced isoform

GTTCTTCTTTACAGAGCAAAGAAAGGCAAAGCAGATTTTAGAAGAAAACAGGAACGGACAGAAAGAAATAGAACCATGTCTGGGCGTGGAAAGGGAGGCAAGGGTTTGGGTAAAGGAGGGGCCAAGAGGCACAGGAAGGTTTTGAGAGACAACATTCAGGGCATAACGAAGCCGGCCATACGGCGTCTTGCCAGGAGGGG
AGGTGTCAAGCGAATCAGCGGCCTTATTTACGAAGAAACGAGGGGGGTTTTGAAGATCTTCTTGGAACATGTTATCAAGGATGCCGTCACTTACACTGAGCACGCCCGAAGGAAGACCGTCACTGCCATGGATGTTGTATATTCCCTCAAGCGGCAGGGCAGGACTCTCTACGGATTCGGTGGTTGAATAACTTCCAATG
GACCGCCCAAACTCTATTTGTTCAAATCCAATCTCTCGGTCCATTTATTTCTCCTTTCATGGTGTTTCTGGACACCACCCGCTGCATCTCTGGAGGTATATCGAAGACTTACATTTACCCAGATGTCTTTGGAGCATTTGCATTTGCTATAGGTATATCGAAGACTTACATTTACCCAGATGTCTTTGGAGCATTCACAT
TTGCTATAGGTATATGGAAGATTTACATTTACCCAGATGTCTTTGGAGCATTTGCTAATCCCTGAGTTAGTGATTAACAGTTATAATTAGATTTAAGAAACGAACAACATAGTATCAGTCTGCAGCATATGAAGATAACATGGGGATGTTTATAATGTAAGTACGTTACGTGATTTTGTTAGTTTCAATTCGCGGTTGAC
AACGATGATGGAAATGATTAAAGAAATTGGTATCAGGGTTTCTGAGCAGTGACTTCTGTTTCCTCTGTTGTAACCACATCGATGTTCTCTCCTTTGTTGCCCAATTCCTTGTTTTTGTCATTGTTTTCCCCATTGTTCCTTATTTTCTAGTCCGAATATTTTTAAAGTATTTTTTTTTTAAACTATGGGAATAGCAATCT
CTCAAGGGTATATCTAAGAGTCTGTAAGTCAGATATACCAGAAATTTGTGATATCCCGAGACTCTTTATCCCAAAGAAGAGCCTCTATCTCAGGGGTTACCTATCCTTTCAACCTTCCAAGTTCTTCTCCAGCGACCTTAAGTTTCTGAGGGACACCTTGACCATGATACGGTTACCATTTCTGACTCCATTAGATATTA
GATTTATACAGAGTATTAGGGTTTACATTGTTGGCTTTTTTAATAAAAATACAGAGAAATCAAACGGGC

>TRINITY_DN42792_c0_g1_i2 from sample A6 / Example unspliced isoform:

GTTCTTCTTTACAGAGCAAAGAAAGGCAAAGCAGATTTTAGAAGAAAACAGGAACGGACAGAAAGAAATAGAACCATGTCTGGGCGTGGAAAGGGAGGCAAGGGTTTGGGTAAAGGAGGGGCCAAGAGGCACAGGAAGGTTTTGAGAGACAACATTCAGGGCATAACGAAGCCGGCCATACGGCGTCTTGCCAGGAGGGG
AGGTGTCAAGCGAATCAGCGGCCTTATTTACGAAGAAACGAGGGGGGTTTTGAAGATCTTCTTGGAACATGTTATCAAGGATGCCGTCACTTACACTGAGCACGCCCGAAGGAAGACCGTCACTGCCATGGATGTTGTATATTCCCTCAAGCGGCAGGGCAGGACTCTCTACGGATTCGGTGGTTGAATAACTTCCAATG
GACCGCCCAAACTCTATTTGTTCAAATCCAATCTCTCGGTCCATTTATTTCTCCTTTCATGGTGTTTCTGGACACCACCCGCTGCATCTCTGGAGgttcttaccttttgctgtctgtcataaccagaagcaaaaatagaaaacacgggattaaaatataaatgtattcggaatgatgtgtgcctcgcaatttgttttggt
tttattgtcgtttgtattcagcaaacacttttgttttcaaatgtcaaaatagtgtgtgaagttttgtccaggattagtaatcaggaaattgtttagggttagtgggaagggtaagaatcttgagggacatagccatggcttatataggcctaaaattgcaagttttttcctttaatttagggacagagctttgctaattt
caaaatttggcaatggttttggtgtaagtttacaataacatattcctggtcaatgtttaagtgtatttattttgaaatttggcaatggttgtgcacctgtacagGTATATCGAAGACTTACATTTACCCAGATGTCTTTGGAGCATTTGCATTTGCTATAGGTATATCGAAGACTTACATTTACCCAGATGTCTTTGGAG
CATTCACATTTGCTATAGGTATATGGAAGATTTACATTTACCCAGATGTCTTTGGAGCATTTGCTAATCCCTGAGTTAGTGATTAACAGTTATAATTAGATTTAAGAAACGAACAACATAGTATCAGTCTGCAGCATATGAAGATAACATGGGGATGTTTATAATGTAAGTACGTTACGTGATTTTGTTAGTTTCAATTC
GCGGTTGACAACGATGATGGAAATGATTAAAGAAATTGGTATCAGGGTTTCTGAGCAGTGACTTCTGTTTCCTCTGTTGTAACCACATCGATGTTCTCTCCTTTGTTGCCCAATTCCTTGTTTTTGTCATTGTTTTCCCCATTGTTCCTTATTTTCTAGTCCGAATATTTTTAAAGTATTTTTTTTTTAAACTATGGGAA
TAGCAATCTCTCAAGGGTATATCTAAGAGTCTGTAAGTCAGATATACCAGAAATTTGTGATATCCCGAGACTCTTTATCCCAAAGAAGAGCCTCTATCTCAGGGGTTACCTATCCTTTCAACCTTCCAAGTTCTTCTCCAGCGACCTTAAGTTTCTGAGGGACACCTTGACCATGATACGGTTACCATTTCTGACTCCAT
TAGATATTAGATTTATACAGAGTATTAGGGTTTACATTGTTGGCTTTTTTAATAAAAATACAGAGAAATCAAACGGGC