NA ----> NA

Intron 1 GTAAGTTTTC ... TGATATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.97	124.7	434.7	550.3
A2	81.22	63.7	250.3	298.7
A3	76.51	103.7	318	349.3
A4	84.64	58.3	314.3	323
A5	73.04	38	93.7	112
A6	82.89	55.3	226.3	304.3
A7	80.55	229	782.3	1078.3
A8	80.81	327.7	1148	1586
A9	81.32	190.7	714.3	930.3
A10	82.67	130.3	501.7	733

>TRINITY_DN123802_c0_g2_i7 from sample A2 / Example spliced isoform

TACTGTTACAGCAGAAATATTTGTTGTTGAGTACACAGTTAGGTTCTGTTCAGTTCAATGATATATCAGCCCTTTGCAGCCAATGTGGTATTCCTCTATGCAGTGTTCATCAATTTCTCAAGCTTTTCATGCATCAGTGCCTACTCCAACCCATCCAGATGCTTAGTAGACTGCACAACTGCCACATCTATCCCCTTGTT
AAAGGTGGAGATCAGTGTCCATATCCATTGTTTCCTCCAGAGATTATACCAGGAATGTTTTTGAAAATGCCAAATGGGAAGTGGCTGCCTTAATGAGCCCTCTGCATATCCGATAGTGATTCCTGCACTTTCATATATCTTTGAAAGAGTTTCACAATTGTGATGCAGATACTGGCACACTTCCTGATGGAAGCCAGGAT
CAACTAGTACGGAAGGTTGGGCAGTGGAGCTACTGCTGATCCAAATGAAGTGAGCCAGTGTTATGGTGTTTATACTTCATACAGGAAGCAAAGGATATCATTATACAAAGGAGATCAAGTCAACAAGTGCCAGAGGTTCAGCAAGGTTGGGCCAGGATCAACTCGTACATGAAGGTTGGGCATTGGAGTTATTGCTGATC
CTAGTTAAGTGAGCCAGGATCAACTAGTACAGGAAGGTTGTCATTGGAGTAACCGCTGATCCTAGTGAAGTGAGACAGTATTATGATGTTTATATTTCATACATGAGATCAAGCTATAAATACATCAATGAGTGCATTTGAATAACAGGATGAGATCAAGCTATAGATACATCAATGAGTGCATTCGAATAACAGGAAGG
TTCGGCATTCGAGTATTATGATGTTATCGAGGGAGAGTATTATGATGTTATCGAGGGAGAGATCCAGCTATGAGTGCCAAAGCTTCAGCAGGATGGTTGACCCTCCTTTTCATTGGCATTTGCATAATGCTGTGTAATGCTTAGTATCAAGGGAGAGATCCAGCTATGAGTGCCAGAGCTTCAGCAGGATGGTTGACACT
CCTTTTCATTGGCATTTGCATAATGCTTTGTAATGTTGTGTAATGCTTAGTAACGTCAACTTTTTGTAAAGCATCACTTGTGAAGTTCACGAGTCCTAACAATTAAACTGTTTTTCTGTGTGTAAACAACAAATTACACAAATTTATGAAATATAAAATGGTGAAGAATTCTTGGTAGAGGAGCATCATTCAATGTAGAA
ATGATCTTGAGATCTTCATGAAGTTCACGAGTCCTAACAGTT

>TRINITY_DN123802_c0_g2_i2 from sample A2 / Example unspliced isoform:

TTAAAGGTGGAGATCAGTGTCCATATCCATTGTTTCCTCCAGAGATTATACCAGGAATGTTTTTGAAAATGCCAAATGGGAAGTGGCTGCCTTACTGAGCCCTCTGCATATCCGATAGTGATTCCTGCACTTTCATATATCTTTGAAAGAGTTTCACAgtaagttttcaatttaatcctttagagagtcagacaacttta
tctggtacaataaaggattatgaaagagatccaaccaagtcgtacaggtggtgatggtattccactgctgcttgatatgcagATTGTGATGCAGATACTGGCACACTTCCTGATGGAAGCCAGGATCAACTAGTACGGAAGGTTGGGCAGTGGAGCTACTGCTGATCCAAATGAAGTGAGCCAGTGTTATGGTGTTTATA
CTTCATACAGGAAGCAAAGGATATCATTATACAAAGGAGATCAAGTCAACAAGTGCCAGAGGTTCAGCAAGGTTGGGCCAGGATCAACTCGTACATGAAGGTTGGGCATTGGAGTTATTGCTGATCCTAGTTAAGTGAGCCAGGATCAACTAGTACAGGAAGGTTGTCATTGGAGTAACCGCTGATCCTAGTGAAGTGAG
ACAGTATTATGATGTTTATATTTCATACATGAGATCAAGCTATAAATACATCAATGAGTGCATTTGAATAACAGGATGAGATCAAGCTATAGATACATCAATGAGTGCATTCGAATAACAGGAAGGTTCGGCATTCGAGTATTATGATGTTATCGAGGGAGAGTATTATGATGTTATCGAGGGAGAGATCCAGCTATGAG
TGCCAAAGCTTCAGCAGGATGGTTGACCCTCCTTTTCATTGGCATTTGCATAATGCTGTGTAATGCTTAGTATCAAGGGAGAGATCCAGCTATGAGTGCCAGAGCTTCAGCAGGATGGTTGACACTCCTTTTCATTGGCATTTGCATAATGCTTTGTAATGTTGTGTAATGCTTAGTAACGTCAACTTTTTGTAAAGCAT
CACTTGTGAAGTTCACGAGTCCTAACAATTAAACTGTTTTTCTGTGTGTAAACAACAAATTACACAAATTTATGAAATATAAAATGGTGAAGAATTCTTGGTAGAGGAGCATCATTCAATGTAGAAATGATCTTGAGATCTTCATGAAGTTCACGAGTCCTAACAGTT

Intron 2 GTAAGTTTTC ... TGATATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.39	105	259	550
A2	79.93	58	164	298
A3	75.63	87	188	352
A4	84.22	49	202	321
A5	71.08	36	65	112
A6	83.48	46	160	305
A7	82.11	179	559	1084
A8	81.01	277	771	1593
A9	81.38	161	473	934
A10	83.55	112	404	734

>TRINITY_DN122753_c0_g1_i14 from sample A3 / Example spliced isoform

TTGCTTAGTAGACTGCACAACTGCCACATCTATCCCCTTGTTAAAGGTGGAGATCAGTGTCCATATCCATTGTTTCCTCCAGAGATTATACCAGGAATGTTTTTGAAAATGCCAAATGGGAAGTGGCTGCCTTAATGAGCCCTCTGCATATCCGATAGTGATTCCTGCACTTTCATATATCTTTGAAAGAGTTTCACAAT
TGTGATGCAGATACTGGCACACTTCCTGATGGAAGCCAGGATCAACTAGTACGGAAGGTTGGGCAGTGGGGAAAGGTTGGCCAGTGGAGCTACTGCTGATCCAAATGAAGTGAGCCAGTGTTATGGTGTTTATACTTCATACAGGAAGCAAAGGATATCA

>TRINITY_DN122753_c0_g1_i7 from sample A3 / Example unspliced isoform:

TAAGGAATTCCAGAAGCATTAGGTTCACAATGAAAGTGTCAGCCATCAACAAAGCAGGAACAGAAAAATAAACCTATACTACTGTTACAGCAGAAATATTTGTTGTTGAGTACACAGTTAGGTTCTGTTCAGTTCAATGATATATCAGCCCTTTGCAGCCAATGTGGTATTCCTCTATGCAGTGTTCATCAATTTCTCAA
GCTTTTCATGCATCAGTGCCTACTCCAACCCATCCAGTTGCTTAGTAGACTGCACAACTGCCACATCTATCCCCTTGTTAAAGGTGGAGATCAGTGTCCATATCCATTGTTTCCTCCAGAGATTATACCAGGAATGTTTTTGAAAATGCCAAATGGGAAGTGGCTGCCTTACTGAGCCCTCTTTCATATATCTTTGAAAG
AGTTTCACAgtaagttttcaattcaatcctttagagtcagacaactttatctggtgcaatagaggatgatgaaagagatccaaccaagtcgtacaggtggtgattgtattccactgctacttgatatgcagATTGTGATGCAGATACTGGCACACTTCCTGATGGAAGCCAGGATCAACTAGTACGGAAGGTTGGGCAGT
GGGGAAAGGTTGGCCAGTGGAGCTACTGCTGATCCAAATGAAGTGAGCCAGTGTTATGGTGTTTATACTTCATACAGGAAGCAAAGGATATCAAGTCAACAAGTGCCAGAGGTTCAGCAAGGTTGGGCCAGGATCAACTAGTACATGAAGGTTGGGCATTGGAGTTATTGCTGATCCTAGTTAAGTGAGCCAGGATCAAC
TAGTACAGGAAGGTTGTCATTGGAGTAACTGCTGATCCTAGTGAAGTGAGCCAGTATTATGATGTTTATATTTCATACATGAGATCAAGCTATAGATACATCAATTAGTGCATTTGAATAAAAGGAAGGTTCGGCATTCGAGTATTATGATGTTATCGAGGGAGAGTATTATGATGTTATCGAGGGAGAGATCCAGCTAT
GAGTGCTAAAGCTTCAGCAGGATGGTTGACCCTCCTTTTCATTGGCATTTGCATAATGCTGTGTAATGTTTAGTATCGAGGGAGAGATGCAGCTATGAGTGCCAAAGCTTCAGCAAGATGGTTGATACTCCTTTTCATTGGCATTTGCATAATGCTTTGTAATGTTGTGTAATGCTTAGTAACGTCAACTTTTTGTAAAG
CATCACTTGTGAAGTTCACGAGTCCTAACAATTAAACTGTTTTTCTGTGTGTAAACAACAAATTACACAAATTTATGAAATATAAAATGGTGAAGAATTCTTGGTAGAGGAGCATCATTCAATGTAGAAATGATCTTGAGATCTTCATGAAGTTCACGAGTCCTAACAGTTGAACAGTTTTTCTGTGTGTAAACAGCAAA
TTACACAAATTTATGAAATATAAAATGGTGAAGAATTCTTGGTAGAGGAGCATCATTCCATGTAGAAATGATCTTGATCTTCATTAGAAAGCACTTTGTATGCTTTTATTTTGACTTGACTTCATGTTGTGTCTACAAATTTTCAGTAACATTTAAATTATAGAGATTGGTTGCTTTGTAAATTGTATCTCTGTTGATGA
CATATTCCAGCATCAAAGAATTGACATATACTAAATATTCCAGCATCAAAGAATTGACATATACTAAATATTCCAGCATCAAAGAATTGA

Intron 3 ATCAACTAGT ... TATTTCATAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	54.69	179	216	30
A2	53.03	101	114	12
A3	53.7	119	138	29
A4	57.5	85	115	24
A5	60.81	29	45	7
A6	52.24	203	222	32
A7	54.08	648.8	764	125
A8	54.26	962.8	1142	151
A9	56.23	441.5	567	99
A10	55.83	417	527	79

>TRINITY_DN123802_c0_g2_i10 from sample A2 / Example spliced isoform

ATTGTGATGCAGATACTGGCACACTTCCTGATGGAAGCCAGGATCAACTAGTACGGAAGGTTGGGCAGTGGAGCTACTGCTGATCCAAATGAAGTGAGCCAGTGTTATGGTGTTTATACTTCATACAGGAAGCAAAGGATATCAAGTCAACAAGTGCCAGAGGTTCAGCAAGGTTGGGCCAGGATCAACTAGTACATGAA
GGTTGGGCATTGGAGTTATTGCTGATCCTAGTTAAGTGAGCCAGGATCAACTAGTACAGGAATGTTGTCATTGGAGTAACCGCTGATCCTAGTGAAGTGAGCCAGTATTATGATGTTTATATTTCATACATGAGATCAAGCTATAAATACATCAATGAGTGCATTTGAATAACAGGATGAGATCAAGCTATAGATACATC
AATTAGTGCATTTGAATAAAAGGAAGGTTCGGCATTCGAGTATTATGATGTTATCGAGGGAGAGTATTATGATGTTATCGAGGGAGAGATCCAGCTATGAGTGCTAAAGCTTCAGCAGGATGGTTGACCCTCCTTTTCATTGGCATTTGCATAATGCTGTGTAATGTTTAGTATCGAGGGAGAGATGCAGC

>TRINITY_DN123802_c0_g2_i6 from sample A2 / Example unspliced isoform:

GTACATGAAGGTTGGGCATTGGAGTTATTGCTGATCCTAGTTAAGTGAGCCAGGATCAACTAGTACAGGAAGGTTGTCATTGGAGTAACCGCTGATCCTAGTGAAGTGAGCCAGTATTATGATGTTTATATTTCATACATGAGATCAAGCTATAAATACATCAATGAGTGCATTTGAATAACAGGatcaactagtacagg
aaggttgtcattggagtaaccgctgatcctagtgaagtgagccagtgttatgatgtttatatttcatacATGAGATCAAGCTATAGATACATCAAT