sp|Q96291|BAS1A_ARATH ----> 2-Cys peroxiredoxin BAS1, chloroplastic

Intron 1 GCATGCTTGA ... TCTGTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.22	173	44	23
A2	17.41	121	37	14
A3	17.28	146	38	23
A4	22.81	66	28	11
A5	20	44	11	11
A6	17.23	610	125	129
A7	17.13	723	168	131
A8	17.95	704	174	134
A9	16.7	591	130	107
A10	19.12	442	113	96

>TRINITY_DN95922_c0_g2_i2 from sample A5 / Example spliced isoform

GTGATAAGTAAGTTCCTATTTAAGTGAGCCTTGCTGCATGTCCCCTATTATCCACATATCTCGAGGGTTTTGTTCTTTCATTTCAGTTTGTTGTTCTTCCATTTCAGTTTGGTGAAGTGGCAACCATGGCCTGTGCAACATCTCCATCTTCACCGCTACTGCTATCGTCTACTCACGAGAAGGTGAGGGCAGGTTGTTCA
TCTTCAGGCAGGTGGAGGGTAACCAAGGGGGGTTTACAAGGCCTGAGGAGTGTACCCAACCGGGGTTTTGAAGGCCTGCGCAGAACCTTCAGTACCCGATTGGCAGGCCCCCGGACCGCTGCCGCCCGCACCTCTAACAATGAGAACTTAGGGAGGAGGCTTGTCGTCAATGCAGGTGAACTTCCATTGGTCGGAAACAA
GGCACCTGATTTCGAAGCAGAGGCTGTTTTTGATCAAGAGTTCATCAAGGTCAAGCTCTCTGAATACATTGGGAAAAAATATGTTATTCTTTTCTTCTATCCGTTGGACTTCACTTTTGTCTGCCCCACAGAAATCACTGCTTTCAGTGACAGACATTCTGAATTTGAGAAGTTGAACACAGAAATTCTTGGTGTTTCTA
TTGATAGTGTGTTCTCCCACCTTGCTTGGGTTCAAACTGATAGGAAAGCTGGAGGTCTCGGTGATTTGAAATATCCTCTTATATCTGATGTTACAAAGGGTATTTCAAAGGCCTACAATGTTTTGATTGCAGATCAGGGAATAGCATTACGAGGACTTTTTATCATCGACAAAGAGGGTGTGATTCAACACTCCACAATC
AACAATCTAGCAATTGGTCGGAGTGTGGATGAAACGTTGCGTACATTACAGGCTCTTCAATATGTTCAGGATAACCCCGATGAAGTTTGCCCAGCTGGGTGGAAGCCAGGAGACAAATCAATGAAGCCAGACCCCAAATTGAGTAAAGAGTATTTCGAAGCTATCTGAAAATCTAGATGAAGATTAAATACGGTGTTGGT
CCATCGAGCTGCATTATTGTCCAAGTAGAGGTTTACTAGGGATATTTAACAATAAGGCATTCAATTTTGATTATCCTCTAAATAGGTAGTTATGTTGAAGTTCCCTATATTGAAGGCCCTGACTGCTAAGATTAACGTTATTTAGTACCTATTTAAGAAATCCATTCATTTTTTTTGTATCCAGCTCATTAGATGCAACC
ATTGTTTGGAATCTTTCCGTTCCAAGTGTTTGATTGCCTTTAGTGTATAAAGAAGTATGTTAGAGGTTATAAAAACAGTGGCACCCATGGTCTATAATAATATCTTCAATTTTTATATATGAACGTGAAAAAACACTATCTTGTGC

>TRINITY_DN95922_c0_g2_i3 from sample A5 / Example unspliced isoform:

AAAATTTAAATTGCGGTTTTGCGTTGGAGAATAATGACATCTTACAGGGCGGTAGTTTTACTGCTAATGTGTTGCATTTTATTTGTTTGCTAATAGGGAATAGCATTACGAGGACTTTTTATCATCGACAAAGAGGGTGTGATTCAACACTCCACAATCAACAATCTAGCAATTGGTCGGAGTGTGGATGAAACGTTGCG
TACATTACAGgcatgcttgaacctaagtttgactttcacactctattgtgaacacaatcgagtgtattgatcactttcagtttgacttgctttagtactgattccctttttctgtgtcagGCTCTTCAATATGTTCAGGATAACCCCGATGAAGTTTGCCCAGCTGGGTGGAAGCCAGGAGACAAATCAATGAAGCCAGA
CCCCAAATTGAGTAAAGAGTATTTCGAAGCTATCTGAAAATCTAGATGAAGATTAAATACGGTGTTGGTCCATCGAGCTGCATTATTGTCCAAGTAGAGGTTTACTAGGGATATTTAACAATAAGGCATTCAATTTTGATTATCCTCTAAATAGGTAGTTATGTTGAAGTTCCCTATATTGAAGGCCCTGACTGCTAAGA
TTAACGTTATTTAGTACCTATTTAAGAAATCCATTCATTTTTTTTGTATCCAGCTCATTAGATGCAACCATTGTTTGGAATCTTTCCGTTCCAAGTGTTTGATTGCCTTTAGTGTATAAAGAAGTATGTTAGAGGTTATAAAAACAGTGGCACCCATGGTCTATAATAATATCTTCAATTTTTATATATGAACGTGAAAA
AACACTATCTTGTGC

Intron 2 GTTGTCACAT ... TTTCTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.15	107	22	32
A2	23.75	61	20	18
A3	24.89	86	24	33
A4	23.5	83	21	30
A5	23.36	41	11	14
A6	20.74	237	56	68
A7	19.08	229	53	55
A8	19.74	244	46	74
A9	22.08	217	58	65
A10	20.31	153	35	43

>TRINITY_DN108438_c9_g1_i4 from sample A3 / Example spliced isoform

AGTGGGTATGTGGTGATAAGTAAGTTCCTATTTAAGTGAGCCTTGCTGCATGTCCCCTATTATCCACATATCTCGAGGGTTTTGTTCTTTCATTTCAGTTTGTTGTTCTTCCATTTCAGTTTGGTGAAGTGGCAACCATGGCCTGTGCAACATCTCCATCTTCACCGCTACTGCTATCGTCTACTCACGAGAAGGTGAGG
GCAGGTTGTTCATCTTCAGGCAGGTGGAGGGTAACCAAGGGGGGTTTACAAGGCCTGAGGAGTGTACCCAACCGGGGTTTTGAAGGCCTGCGCAGAACCTTCAGTACCCGATTGGCAGGCCCCCGGACCGCTGCCGCCCGCACCTCTAACAATGAGAACTTAGGGAGGAGGCTTGTCGTCAATGCAGGTGAACTTCCATT
GGTCGGAAACAAGGCACCTGATTTCGAAGCAGAGGCTGTTTTTGATCAAGAGTTCATCAAGGTCAAGCTCTCTGAATACATTGGGAAAAAATATGTTATTCTTTTCTTCTATCCGTTGGACTTCACTTTTGTCTGCCCCACAGAAATCACTGCTTTCAGTGACAGACATTCTGAATTTGAGAAGTTGAACACAGAAATTC
TTGGTGTTTCTATTGATAGTGTGTTCTCCCACCTTGCTTGGGTTCAAACTGATAGGAAAGCTGGAGGTCTCGGTGATTTGAAATATCCTCTTATATCTGATGTTACAAAGGGTATTTCAAAGGCCTACAATGTTTTGATTGCAGATCAGGGAATAGCATTACGAGGACTTTTTATCATCGACAAAGAGGGTGTGATTCAA
CACTCCACAATCAACAATCTAGCAATTGGTCGGAGTGTGGATGAAACGTTGCGTACATTACAGGCTCTTCAATATGTTCAGGATAACCCCGATGAAGTTTGCCCAGCTGGGTGGAAGCCAGGAGACAAATCAATGAAGCCAGACCCCAAATTGAGTAAAGAGTATTTCGAAGCTATCTGAAAATCTAGATGAAGATTAAA
TACGGTGTTGGTCCATCGAGCTGCATTATTGTCCAAGTAGAGGTTTACTAGGGATATTTAACAATAAGGCATTCAATTTTGATTATCCTCTAAATAGGTAGTTATGTTGAAGTTCCCTATATTGAAGGCCCTGACTGCTAAGATTAACGTTATTTAGTACCTATTTAAGAAATCCATTCATTTTTTTTGTATCCAGCTCA
TTAGATGCAACCATTGTTTGGAATCTTTCCGTTCCAAGTGTTTGATTGCCTTTAGTGTATAAAGAAGTATGTTAGAGGTTATAAAAACAGTGGCACCCATGGTCTATAATAATATCTTCAATTTTTATATATGAACGTGAAAAAACACTATCTTGTGCAAATTTCTTATTCTGATATGAATTTGTTTGGGTTGCATTCAT
TCGCCATTGTTTAATGAAACAATTAACGTTATAATGCATTAGAG

>TRINITY_DN108438_c9_g1_i2 from sample A3 / Example unspliced isoform:

AGTGGGTATGTGGTGATAAGTAAGTTCCTATTTAAGTGAGCCTTGCTGCATGTCCCCTATTATCCACATATCTCGAGGGTTTTGTTCTTTCATTTCAGTTTGTTGTTCTTCCATTTCAGTTTGGTGAAGTGGCAACCATGGCCTGTGCAACATCTCCATCTTCACCGCTACTGCTATCGTCTACTCACGAGAAGGTGAGG
GCAGGTTGTTCATCTTCAGGCAGGTGGAGGGTAACCAAGGGGGGTTTACAAGGCCTGAGGAGTGTACCCAACCGGGGTTTTGAAGGCCTGCGCAGAACCTTCAGTACCCGATTGGCAGGCCCCCGGACCGCTGCCGCCCGCACCTCTAACAATGAGAACTTAGGGAGGAGGCTTGTCGTCAATGCAGGTGAACTTCCATT
GGTCGGAAACAAGGCACCTGATTTCGAAGCAGAGGCTGTTTTTGATCAAGAGTTCATCAAGGTCAAGCTCTCTGAATACATTGGGAAAAAATATGTTATTCTTTTCTTCTATCCGTTGGACTTCACTTTTGTCTGCCCCACAGgttgtcacatgatcggatatctttcttgtttgcattacttgaaaagtattagttcaa
gaagagttttctaagcattttcaaaaatataaaaaagcgaatatagattttcataattcagggcggtggttcatcaacttatgaatttcctattatgtttggtttctggcagAAATCACTGCTTTCAGTGACAGACATTCTGAATTTGAGAAGTTGAACACAGAAATTCTTGGTGTTTCTATTGATAGTGTGTTCTCCCA
CCTTGCTTGGGTTCAAACTGATAGGAAAGCTGGAGGTCTCGGTGATTTGAAATATCCTCTTATATCTGATGTTACAAAGGGTATTTCAAAGGCCTACAATGTTTTGATTGCAGATCAGGGAATAGCATTACGAGGACTTTTTATCATCGACAAAGAGGGTGTGATTCAACACTCCACAATCAACAATCTAGCAATTGGTC
GGAGTGTGGATGAAACGTTGCGTACATTACAGGCTCTTCAATATGTTCAGGATAACCCCGATGAAGTTTGCCCAGCTGGGTGGAAGCCAGGAGACAAATCAATGAAGCCAGACCCCAAATTGAGTAAAGAGTATTTCGAAGCTATCTGAAAATCTAGATGAAGATTAAATACGGTGTTGGTCCATCGAGCTGCATTATTG
TCCAAGTAGAGGTTTACTAGGGATATTTAACAATAAGGCATTCAATTTTGATTATCCTCTAAATAGGTAGTTATGTTGAAGTTCCCTATATTGAAGGCCCTGACTGCTAAGATTAACGTTATTTAGTACCTATTTAAGAAATCCATTCATTTTTTTTGTATCCAGCTCATTAGATGCAACCATTGTTTGGAATCTTTCCG
TTCCAAGTGTTTGATTGCCTTTAGTGTATAAAGAAGTATGTTAGAGGTTATAAAAACAGTGGCACCCATGGTCTATAATAATATCTTCAATTTTTATATATGAACGTGAAAAAACACTATCTTGTGCAAATTTCTTATTCTGATATGAATTTGTTTGGGTTGCATTCATTCGCCATTGTTTAATGAAACAATTAACGTTA
TAATGCATTAGAG

Intron 3 GTAAGTTCCT ... TCCATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.52	43	7	108
A2	82.01	25	4	114
A3	73.11	57	6	155
A4	73.47	39	2	108
A5	74.31	28	1	81
A6	75.46	66	17	203
A7	75.37	84	22	257
A8	78.29	56	5	202
A9	75.95	76	19	240
A10	80.08	53	27	213

>TRINITY_DN31529_c0_g1_i1 from sample A9 / Example spliced isoform

AGGGACCTAAAATTCGTCATATGACCAACTCCCAACATCATCACAGCTATATGATGAGGCATAAAGCAGAGCACACCCACATCCGCAAGAGAGAGTGAGGGTAGTGGACTAGTGAGAATATTCCCCCCCTGTTCGTTGTATTCTCCACCCCTCATCGAAGTGGGTATGTGGTGATAATTTGGTGAAGTGGCAACCATGGC
CTGTGCAACATCTCCATCTTCACCGCTACTGCTATCGTCTACTCACGAGAAGGTGAGGGCAGGTTGTTCATCTTCAGGCAGGTGGAGGGTAACCAAGGGGGGTTTACAAGGCCTGAGGAGTGTACCCAACCGGGGTTTTGAAGGCCTGCGCAGAACCTTCAGTACCCGATTGGCAGGCCCCCGGACCGCTGCCGCCCGCA
CCTCTAACAATGAGAACTTAGGGAGGAGGCTTGTCGTCAATGCAGGTGAACTTCCATTGGTCGGAAACAAGGCACCTGATTTCGAAGCAGAGGCTGTTTTTGATCAAGAGTTCATCAAGGTCAAGCTCTCTGAATACATTGGGAAAAAATATGTTATTCTTTTCTTCTATCCGTTGGACTTCACTTTTGTCTGCCCCACA
GAAATCACTGCTTTCAGTGACAGACATTCTGAATTTGAGAAGTTGAACACAGAAATTCTTGGTGTTTCTATTGATAGTGTGTTCTCCCACCTTGCTTGGGTTCAAACTGATAGGAAAGCTGGAGGTCTCGGTGATTTGAAATATCCTCTTATATCTGATGTTACAAAGGGTATTTCAAAGGCCTACAATGTTTTGATTGC
AGATCAGGGAATAGCATTACGAGGACTTTTTATCATCGACAAAGAGGGTGTGATTCAACACTCCACAATCAACAATCTAGCAATTGGTCGGAGTGTGGATGAAACGTTGCGTACATTACAGGCTCTTCAATATGTTCAGGATAACCCCGATGAAGTTTGCCCAGCTGGGTGGAAGCCAGGAGACAAATCAATGAAGCCAG
ACCCCAAATTGAGTAAAGAGTATTTCGAAGCTATCTGAAAATCTAGATGAAGATTAAATACGGTGTTGGTCCATCGAGCTGCATTATTGTCCAAGTAGAGGTTTACTAGGGATATTTAACAATAAGGCATTCAATTTTGATTATCCTCTAAATAGGTAGTTATGTTGAAGTTCCCTATATTGAAGGCCCTGACTGCTAAG
ATTAACGTTATTTAGTACCTATTTAAGAAATCCATTCATTTTTTTTGTATCCAGCTCATTAGATGCAACCATTGTTTGGAATCTTTCCGTTCCAAGTGTTTGATTGCCTTTAGTGTATAAAGAAGTATGTTAGAGGTTATAAAAACAGTGGCACCCATGGTCTATAATAATATCTTCAATTTTTATATATGAACGTGAAA
AAACACTATCTTGTGCAAATTTCTTATTCTGATATGAATTTGTTTGGGTTGCATTCATTCGCCATTGTTTAATGAAACAATTAACGTTATAATGCATTAGAGGTGCTCAAAACTTGAGAAGTAAATAAAAAATTGATGGTTG

>TRINITY_DN31529_c0_g1_i2 from sample A9 / Example unspliced isoform:

AGGGACCTAAAATTCGTCATATGACCAACTCCCAACATCATCACAGCTATATGATGAGGCATAAAGCAGAGCACACCCACATCCGCAAGAGAGAGTGAGGGTAGTGGACTAGTGAGAATATTCCCCCCCTGTTCGTTGTATTCTCCACCCCTCATCGAAGTGGGTATGTGGTGATAAgtaagttcctatttaagtgagcc
ttgctgcatgtcccctattatccacatatctcgagggttttgttctttcatttcagtttgttgttcttccatttcagTTTGGTGAAGTGGCAACCATGGCCTGTGCAACATCTCCATCTTCACCGCTACTGCTATCGTCTACTCACGAGAAGGTGAGGGCAGGTTGTTCATCTTCAGGCAGGTGGAGGGTAACCAAGGGG
GGTTTACAAGGCCTGAGGAGTGTACCCAACCGGGGTTTTGAAGGCCTGCGCAGAACCTTCAGTACCCGATTGGCAGGCCCCCGGACCGCTGCCGCCCGCACCTCTAACAATGAGAACTTAGGGAGGAGGCTTGTCGTCAATGCAGGTGAACTTCCATTGGTCGGAAACAAGGCACCTGATTTCGAAGCAGAGGCTGTTTT
TGATCAAGAGTTCATCAAGGTCAAGCTCTCTGAATACATTGGGAAAAAATATGTTATTCTTTTCTTCTATCCGTTGGACTTCACTTTTGTCTGCCCCACAGAAATCACTGCTTTCAGTGACAGACATTCTGAATTTGAGAAGTTGAACACAGAAATTCTTGGTGTTTCTATTGATAGTGTGTTCTCCCACCTTGCTTGGG
TTCAAACTGATAGGAAAGCTGGAGGTCTCGGTGATTTGAAATATCCTCTTATATCTGATGTTACAAAGGGTATTTCAAAGGCCTACAATGTTTTGATTGCAGATCAGGTTTGTATGTTTGGTATATCTGTGCCTTTGTAATAATGGAAGTATCAAAATTCAGACAGAATGCAGCATAATATATA