sp|Q6ZCX3|FH6_ORYSJ ----> Formin-like protein 6

Intron 1 GCCCCCAGAA ... GGTGGATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.94	25	30	48
A2	61.29	12	17	21
A3	71.93	8	23	18
A4	60.61	13	11	29
A5	57.14	3	4	4
A6	50	3	1	5
A7	60.49	16	25	24
A8	70.37	4	8	11
A9	73.58	7	16	23
A10	72	7	18	18

>TRINITY_DN46164_c0_g1_i2 from sample A8 / Example spliced isoform

CTTGCACCTTCCCCACCTCCTCCTCCTCCTCTTCCTCGATCTGGACCTCTTGCAACACCCCAACCTCCTCCTTTACCCCCTGGAGCCGGTGGAATCCGTAACCCTCCCCCAGCTCCACCTTTGCCACCTTTTGTGTCAAGTGGGGGAGCCCCTCCTCCTCCACCCCCTCCTCTTGGAGGACGCAACATTCCTCCACCACC
ACCACCATCAAGAGGTCATGGAGTTCCCCCATCACCGCCATCACTAGCTGGAAATTTTCCAAATGGTGCCATAGGAGCTTTTGCACCTCCACCACCTCCTCAGGGAAGAGGCCGAGGATCTGTAAGCCGTGGTGGATTTATGTCTCCTTCAACGCCTAGACGAGCATCATTGAAGCCATTGCACTGGATCAAAGTAACAA
GGGCAGTGAAGGGCAGCTTATGGGCAGAGGCTCAGATGTATGATGAAGCAATAAAGTGAAAGATCAGGAGGACGTCGCACAGCAAGAGGGGCTAAATCAGATAGAGTGCATTTGGTTGATTTAAGAAGGGCTAATAACTGCGAAATTATGCTTACTAAGGTCAAAATGCCATTACCGGATGTAATGAGTGCAGTACTGGC
TTTGGATGACACAGTTTTAGATATTGATCAGGTAGAAAACTTGATAAAGTTTTGCCCCACAAAAGAAGAAATGGATCTCCTCAAGGGATATAATGGAGAGAGAGAGAATCTGGGCAAATGTGAGCAGTTTTTCTTGGAGATGATGAAG

>TRINITY_DN46164_c0_g1_i1 from sample A8 / Example unspliced isoform:

CTTGCACCTTCCCCACCTCCTCCTCCTCCTCTTCCTCGATCTGGACCTCTTGCAACACCCCAACCTCCTCCTTTACCCCCTGGAGCCGGTGGAATCCGTAACCCTCCCCCAGCTCCACCTTTGCCACCTTTTGTGTCAAGTGGGGGAGCCCCTCCTCCTCCACCCCCTCCTCTTGGAGGACGCAACATTCCTCCACCACC
ACCACCATCAAGAGGTCATGGAGTTCCCCCATCACCGCCATCACTAGCTGGAAATTTTCCAAATGGTGCCATAGGAGCTTTTGCACCTCCACCACCTCCTCAGGGAAGAGGCCGAGGATCTGTAAGCCGTGGTGGATTTATGTCTCCTTCAACGCCTAGACGAGCATCATTGAAGCCATTGCACTGGATCAAAGTAACAA
GGGCAGTGAAGGGCAGCTTATGGGCAGAGGCTCAGATGTATGATGAAGCAATAAAgcccccagaatttgacatgtcagaacttgagaatctgttttctgctgctgtgccaacttctggtcatggtggatcagGTGAAAGATCAGGAGGACGTCGCACAGCAAGAGGGGCTAAATCAGATAGAGTGCATTTGGTTGATTTA
AGAAGGGCTAATAACTGCGAAATTATGCTTACTAAGGTCAAAATGCCATTACCGGATGTAATGAGTGCAGTACTGGCTTTGGATGACACAGTTTTAGATATTGATCAGGTAGAAAACTTGATAAAGTTTTGCCCCACAAAAGAAGAAATGGATCTCCTCAAGGGATATAATGGAGAGAGAGAGAATCTGGGCAAATGTGA
GCAGTTTTTCTTGGAGATGATGAAG

Intron 2 GCTATTGAAA ... ATTTGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.14	27	37.9	63
A2	88.64	5	37	41
A3	64.86	13	20	28
A4	63.41	15	17	35
A5	61.11	7	8	14
A6	67.03	15	22	39
A7	65.33	26	30	68
A8	78.1	15	47	60
A9	68.79	27	49	70
A10	62.03	15	21	28

>TRINITY_DN126477_c2_g1_i15 from sample A1 / Example spliced isoform

GCTGGAAATTTTCCAAATGGTGCCATAGGAGCTTTTGCACCTCCACCACCTCCTCAGGGAAGAGGCCGAGGATCTGTAAGCCGTGGTGGATTTATGTCTCCTTCAACGCCTAGACGAGCATCATTGAAGCCATTGCACTGGATCAAAGTAACAAGGGCAGTGAAGGGCAGCTTATGGGCAGAGGCTCAGATGTATGATGA
AGCAATAAAGCCCCCAGAATTTGACATGTCAGAACTTGAGAATCTGTTTTCTGCTGCTGTGCCAACTTCTGGTCATGGTGGATCAGGTGAAAGATCAGGAGGACGTCGCACAGCAAGAGGGGCTAAATCAGATAGAGTGCATTTGGTAATGTGTCCATGCCTGCACTATGATGAGGTTGATTTAAGAAGGGCTAATAACT
GCGAAATTATGCTTACTAAGGTCAAAATGCCATTACCGGATGTAATGAGTGCAGTACTGGCTTTGGATGACACAGTTTTAGATATTGATCAGGTAGAAAACTTGATAAAGTTTTGCCCCACAAAAGAAGAAATGGATCTCCTCAAGGGATATAATGGAGAGAGAGAGAATCTGGGCAAATGTGAGCAGTTTTTCTTGGAG
ATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTATGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACG
GGGTTCTGCGATTGGCTTTAGGCTAGATAGTCTTCTGAAATTGATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAGGTTCTTGCTGAGAAACTTCCAGAGCTCCTTGATTTCAATAAGGATCTTGCTCATTTGGAAGCTGCATCAAAGAGACAATTGAAGACATTAGCAGAGGAGATGC
AAGCTGTAAGCAAAGGTATAGAAAAGGTTGAGCAGGAACTAACTGCATCAGAGAATGATGGTCCAGTATCTGAAGGGTTTCGGAAGTGGTTTCTACTCTTCTCAACTTTGTGACAATGTTTCGGCGGGCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGA
TGAAACTTTCTCCCTTGAGAATAGATGCTGGAGAACAGCCTTCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCTTTTTGGCTTATTGTAACACTAAAGTGCATATTCATTTTTGCCCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAAT
GACACAGATATCATTTACCCACATGCCAATTGTGCTTGTAAAACTATCAAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTACTATAGGCTGAACAAGTATATGGGGCAACAGCAAAAATAAGGGCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAAT
TTTGATGTAATGATAGCACACCATTTGTTTCGGATATTCGATAAACTAATTGATCATCCCAGGAATGTGCTTGAAGAGATGCATTGTGTTTATTCAGTACTTCCCTCTTTGAGAATATCCTCAGATTCAGTTCTTATCTGCAGTCCTCAATTCTTCAACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTT
ACTCTTCCAAGAATATATTTTTGCTGGAGAGAACAACAACAAAGGGTATACCGACTCTTCTTTTATGAGTGCCAGGCGAAGGGCTTATTCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTGGTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATA
ATTGTTAGATAAAATTTTAAGCTCCCTTGTGAAATAAGAAACACATTGGGTAAACTTGCCAAGTTGCCATGTTTATCAGCATTGTAAACTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATATTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTC
TTTCTCTAGGCATTGGCTGCAACAGGTAAATTTTTACATGATCAAGATGCATACTGTAGATAGTATAGAATTTGAACAGCATACCAAGCTTGTCAAAGAAATGAGAAATGGCAAGACTGAAACAATGGTGGAAGGCTGGAAAAGGGGGAAAATTT

>TRINITY_DN126477_c2_g1_i3 from sample A1 / Example unspliced isoform:

CATAACCAGATTAATGTTTTTTGAATGCGGGGTCATTGCAGTTTTTCTTGGAGATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTCTGTGGACAATACTCATTTTTCATTTCCAGTTCAGCTTTCGTAAGTGAAATAGAAGTTCTAGACTTATGTGAGAAA
CACCTTGCACTACAATTAATGCTGTTTGTTGTTTTATTTTTTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTATGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACGGGGTTCTGCGATTGGCTTTAGGCT
AGATAGTCTTCTGAAATTGATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAGGTTCTTGCTGAGAAACTTCCAGAGCTCCTTGATTTCAATAAGGATCTTGCTCATTTGGAAGCTGCATCAAAGAGACAATTGAAGACATTAGCAGAGGAGATGCAAGCTGTAAGCAAAGGTATAGAAA
AGGTTGAGCAGGAACTAACTGCATCAGAGAATGATGGTCCAGTATCTGAAGGGTTTCGGAAgctattgaaaggtttccttgttgatgctgaagctgatgtgaaatctctgacctacctctattcagaagtgggtcggaatgcagatgcacttgctcttttttttggggaagaccctgcacgatgtccatttgaacagGTG
GTTTCTACTCTTCTCAACTTTGTGACAATGTTTCGGCGGGCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGATGAAACTTTCTCCCTTGAGAATAGATGCTGGAGAACAGCCTTCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCTTT
TTGGCTTATTGTAACACTAAAGTGCATATTCATTTTTGCCCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAATGACACAGATATCATTTACCCACATGCCAATTGTGCTTGTAAAACTATCAAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTACTA
TAGGCTGAACAAGTATATGGGGCAACAGCAAAAATAAGGGCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAATTTTGATGTAATGATAGCACACCATTTGTTTCGGATATTCGATAAACTAATTGATCATCCCAGGAATGTGCTTGAAGAGATGCATTGTG
TTTATTCAGTACTTCCCTCTTTGAGAATATCCTCAGATTCAGTTCTTATCTGCAGTCCTCAATTCTTCAACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTTACTCTTCCAAGAATATATTTTTGCTGGAGAGAACAACAACAAAGGGTATACCGACTCTTCTTTTATGAGTGCCAGGCGAAGGGCTTAT
TCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTGGTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATAATTGTTAGATAAAATTTTAAGCTCCCTTGTGAAATAAGAAACACATTGGGTAAACTTGCCAAGTTGCCATGTTTATCAGCATTGTAAA
CTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATATTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTCTTTCTCTAGGCATTGGCTGCAACAGGTAAATTTTTACATGATCAAGATGCATACTGTAGATAGTATAGAATTTGAACAGCATACCAAG
CTTGTCAAAGAAATGAGAAATGGCAAGACTGAAACAATGGTGGAAGGCTGGAAAAGGGGGAAAATTT

Intron 3 GTAAGTTAAA ... ATGATTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.7	48	14	0
A2	25.88	40.2	14	5
A3	13.25	39.3	6	7.5
A4	12.04	43.8	6	0
A5	24.76	18.3	6	4
A6	5.23	18.2	1	0
A7	5.13	55.4	3	0
A8	3.81	50.5	2	0
A9	11.59	30.6	4	2
A10	11.71	37.8	5	0

>TRINITY_DN114463_c1_g1_i7 from sample A2 / Example spliced isoform

ACCAGATTAATGTTTTTTGAATGCGGGGTCATTGCAGTTTTTCTTGGAGATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTA
TGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACGGGGTTCTGCGATTGGCTTTAGGCTAGATAGTCTTCTGAAATTGATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAGGTTCTTGCTGAGAAACTTCCAGAGCTCCTTGATTTCAATAAGGATCTTGCTCAT
TTGGAAGCTGCATCAAAGAGACAATTGAAGACATTAGCAGAGGAGATGCAAGCTGTAAGCAAAGGTATAGAAAAGGTTGAGCAGGAACTAACTGCATCAGAGAATGATGGTCCAGTATCTGAAGGGTTTCGGAAGCTATTGAAAGGTTTCCTTGTTGATGCTGAAGCTGATGTGAAATCTCTGACCTACCTCTATTCAGA
AGTGGGTCGGAATGCAGATGCACTTGCTCTTTTTTTTGGGGAAGACCCTGCACGATGTCCATTTGAACAGGTGGTTTCTACTCTTCTCAACTTTGTGACAATGTTTCGGCGGGCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGATGAAACTTTCTCCCT
TGAGAATAGATGCTGGAGAACAGCCTTCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCTTTTTGGCTTATTGTAACACTAAAGTGCATATTCATTTTTGCCCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAATGACACAGATATCGTT
TACCAACATGCCAATTGTGCTTGTAAAACTATCGAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTACTATAGGCTGAACAAGTATATGGGGCAACAGCAAAAATAAGGGCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAATTTTGATGTAATGATA
GCACACCATTTGTTTCGGATATTCGATAAACTAATTGATCATCCCAGGAATGTGCTTGAAGAGATGCATTGTGTTTATTCAGTACTTCCCTCTTTGAGAATATCCTCAGATTCAGTTCTTATCTGCAGTCCTCAATTCTTCAACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTTACTCTTCCAAGAATA
TATTTTTGCTGGAGAGAACAACAACAAAGGGTATACCGACTCTTCTTTTATGAGTGCCAGGCGAAGGGCTTATTCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTGGTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATAATTGTTAGATAAAAT
TTTAAGCTCCCTTACGAAATAAGAAACACATTGGGTAAACTTGCCAAGTTGCCATGTTTATCAGCATTGTAAACTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATATTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTCTTTCTC

>TRINITY_DN114463_c1_g1_i11 from sample A2 / Example unspliced isoform:

ACCAGATTAATGTTTTTTGAATGCGGGGTCATTGCAGTTTTTCTTGGAGATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTA
TGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACGGGgtaagttaaagtgatttctacctggtttgttccccatctttggaaataatgtttgtaggtgggagaggaataagagagaaaaagttagtatattcaactattcttcaaatcatatatgaactaacctccaccttctgttcctttcctcc
atgtgtcattggagacaaattgaagtgctgggcaatatatattttggatttcctgtatttagaatattgcagccctattggttctctttaatctgattgttgctttacatacttctattactttaatcactatttttctcactgatttctatgattttagGTTCTGCGATTGGCTTTAGGCTAGATAGTCTTCTGAAATT
GATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAG

Intron 4 GTAATGTGTC ... CTATGATGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.81	43	21	21
A2	28.3	38	14	16
A3	38.1	13	6	10
A4	18.31	29	10	3
A5	41.67	7	3	7
A6	10	9	0	2
A7	21.13	28	5	10
A8	24.32	14	2	7
A9	31.34	23	8	13
A10	34.55	18	9	10

>TRINITY_DN126477_c2_g1_i11 from sample A1 / Example spliced isoform

GCTGGAAATTTTCCAAATGGTGCCATAGGAGCTTTTGCACCTCCACCACCTCCTCAGGGAAGAGGCCGAGGATCTGTAAGCCGTGGTGGATTTATGTCTCCTTCAACGCCTAGACGAGCATCATTGAAGCCATTGCACTGGATCAAAGTAACAAGGGCAGTGAAGGGCAGCTTATGGGCAGAGGCTCAGATGTATGATGA
AGCAATAAAGCCCCCAGAATTTGACATGTCAGAACTTGAGAATCTGTTTTCTGCTGCTGTGCCAACTTCTGGTCATGGTGGATCAGGTGAAAGATCAGGAGGACGTCGCACAGCAAGAGGGGCTAAATCAGATAGAGTGCATTTGGTTGATTTAAGAAGGGCTAATAACTGCGAAATTATGCTTACTAAGGTCAAAATGC
CATTACCGGATGTAATGAGTGCAGTACTGGCTTTGGATGACACAGTTTTAGATATTGATCAGGTAGAAAACTTGATAAAGTTTTGCCCCACAAAAGAAGAAATGGATCTCCTCAAGGGATATAATGGAGAGAGAGAGAATCTGGGCAAATGTGAGCAGTTTTTCTTGGAGATGATGAAAGTTCCAAGAGTGGAGTCTAAA
TTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTATGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACGGGGTTCTGCGATTGGCTTTAGGCTAGATAG
TCTTCTGAAATTGATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAGGTTCTTGCTGAGAAACTTCCAGAGCTCCTTGATTTCAATAAGGATCTTGCTCATTTGGAAGCTGCATCAAAGAGACAATTGAAGACATTAGCAGAGGAGATGCAAGCTGTAAGCAAAGGTATAGAAAAGGTTG
AGCAGGAACTAACTGCATCAGAGAATGATGGTCCAGTATCTGAAGGGTTTCGGAAGCTATTGAAAGGTTTCCTTGTTGATGCTGAAGCTGATGTGAAATCTCTGACCTACCTCTATTCAGAAGTGGGTCGGAATGCAGATGCACTTGCTCTTTTTTTTGGGGAAGACCCTGCACGATGTCCATTTGAACAGGTGGTTTCT
ACTCTTCTCAACTTTGTGACAATGTTTCGGCGGGCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGATGAAACTTTCTCCCTTGAGAATAGATGCTGGAGAACAGCCTTCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCTTTTTGGCT
TATTGTAACACTAAAGTGCATATTCATTTTTGCCCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAATGACACAGATATCATTTACCCACATGCCAATTGTGCTTGTAAAACTATCAAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTACTATAGGCT
GAACAAGTATATGGGGCAACAGCAAAAATAAGGGCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAATTTTGATGTAATGATAGCACACCATTTGTTTCGGATATTCGATAAACTAATTGATCATCCCAGATTCAGTTCTTATCTGCAGTCCTCAATTCTTC
AACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTTACTCTTCCAAGAATATATTTTTGCTGGAGAGAACAACAACAAAGGGTATACCGACTCTTCTTTTATGAGTGCCAGGCGAAGGGCTTATTCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTG
GTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATAATTGTTAGATAAAATTTTAAGCTCCCTTGTGAAATAAGAAACACATTGGGTAAACTTGCCAAGTTGCCATGTTTATCAGCATTGTAAACTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATA
TTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTCTTTCTCTAGGCATTGGCTGCAACAGGTAAATTTTTACATGATCAAGATGCATACTGTAGATAGTATAGAATTTGAACAGCATACCAAGCTTGTCAAAGAAATGAGAAATGGCAAGACTGAAACAATGGTGGAAGGCTGGAAAAGGGGGAAAATTT

>TRINITY_DN126477_c2_g1_i15 from sample A1 / Example unspliced isoform:

GCTGGAAATTTTCCAAATGGTGCCATAGGAGCTTTTGCACCTCCACCACCTCCTCAGGGAAGAGGCCGAGGATCTGTAAGCCGTGGTGGATTTATGTCTCCTTCAACGCCTAGACGAGCATCATTGAAGCCATTGCACTGGATCAAAGTAACAAGGGCAGTGAAGGGCAGCTTATGGGCAGAGGCTCAGATGTATGATGA
AGCAATAAAGCCCCCAGAATTTGACATGTCAGAACTTGAGAATCTGTTTTCTGCTGCTGTGCCAACTTCTGGTCATGGTGGATCAGGTGAAAGATCAGGAGGACGTCGCACAGCAAGAGGGGCTAAATCAGATAGAGTGCATTTGgtaatgtgtccatgcctgcactatgatgagGTTGATTTAAGAAGGGCTAATAACT
GCGAAATTATGCTTACTAAGGTCAAAATGCCATTACCGGATGTAATGAGTGCAGTACTGGCTTTGGATGACACAGTTTTAGATATTGATCAGGTAGAAAACTTGATAAAGTTTTGCCCCACAAAAGAAGAAATGGATCTCCTCAAGGGATATAATGGAGAGAGAGAGAATCTGGGCAAATGTGAGCAGTTTTTCTTGGAG
ATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTATGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACG
GGGTTCTGCGATTGGCTTTAGGCTAGATAGTCTTCTGAAATTGATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAGGTTCTTGCTGAGAAACTTCCAGAGCTCCTTGATTTCAATAAGGATCTTGCTCATTTGGAAGCTGCATCAAAGAGACAATTGAAGACATTAGCAGAGGAGATGC
AAGCTGTAAGCAAAGGTATAGAAAAGGTTGAGCAGGAACTAACTGCATCAGAGAATGATGGTCCAGTATCTGAAGGGTTTCGGAAGTGGTTTCTACTCTTCTCAACTTTGTGACAATGTTTCGGCGGGCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGA
TGAAACTTTCTCCCTTGAGAATAGATGCTGGAGAACAGCCTTCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCTTTTTGGCTTATTGTAACACTAAAGTGCATATTCATTTTTGCCCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAAT
GACACAGATATCATTTACCCACATGCCAATTGTGCTTGTAAAACTATCAAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTACTATAGGCTGAACAAGTATATGGGGCAACAGCAAAAATAAGGGCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAAT
TTTGATGTAATGATAGCACACCATTTGTTTCGGATATTCGATAAACTAATTGATCATCCCAGGAATGTGCTTGAAGAGATGCATTGTGTTTATTCAGTACTTCCCTCTTTGAGAATATCCTCAGATTCAGTTCTTATCTGCAGTCCTCAATTCTTCAACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTT
ACTCTTCCAAGAATATATTTTTGCTGGAGAGAACAACAACAAAGGGTATACCGACTCTTCTTTTATGAGTGCCAGGCGAAGGGCTTATTCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTGGTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATA
ATTGTTAGATAAAATTTTAAGCTCCCTTGTGAAATAAGAAACACATTGGGTAAACTTGCCAAGTTGCCATGTTTATCAGCATTGTAAACTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATATTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTC
TTTCTCTAGGCATTGGCTGCAACAGGTAAATTTTTACATGATCAAGATGCATACTGTAGATAGTATAGAATTTGAACAGCATACCAAGCTTGTCAAAGAAATGAGAAATGGCAAGACTGAAACAATGGTGGAAGGCTGGAAAAGGGGGAAAATTT

Intron 5 GCAAGTTATT ... GTTTGGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.95	29	1	13
A2	28.57	20	0	8
A3	33.33	16	0	8
A4	15.38	22	2	4
A5	33.33	4	0	2
A6	25.71	52	1	18
A7	26.53	72	2	26
A8	26.51	122	2	44
A9	26.21	76	1	27
A10	22.86	27	6	8

>TRINITY_DN47769_c0_g3_i5 from sample A7 / Example spliced isoform

CTGCACCTTCCCCACCTCCTCCTCCTCCTCTTCCTCGATCTGGACCTCTTGCAACACCCCAACCTCCTCCTTTACCCCCTGGAGCCGGTGGAATCCGTAACCCTCCCCCAGCTCCACCTTTGCCACCTTTTGTGTCAAGTGGGGGAGCCCCTCCTCCTCCACCCCCTCCTCTTGGAGGACGCAACATTCCTCCACCACCA
CCACCATCAAGAGGTCATGGAGTTCCCCCATCACCGCCATCACTAGCTGGAAATTTTCCAAATGGTGCCATAGGAGCTTTTGCACCTCCACCACCTCCTCAGGGAAGAGGCCGAGGATCTGTAAGCCGTGGTGGATTTATGTCTCCTTCAACGCCTAGACGAGCATCATTGAAGCCATTGCACTGGATCAAAGTAACAAG
GGCAGTGAAGGGCAGCTTATGGGCAGAGGCTCAGATGTATGATGAAGCAATAAAGCCCCCAGAATTTGACATGTCAGAACTTGAGAATCTGTTTTCTGCTGCTGTGCCAACTTCTGGTCATGGTGGATCAGGTGAAAGATCAGGAGGACGTCGCACAGCAAGAGGGGCTAAATCAGATAGAGTGCATTTGGTAATGTGTC
CATGCCTGCACTATGATGAGGTGTAATTATGTTGATTTAAGAAGGGCTAATAACTGCGAAATTATGCTTACTAAGGTCAAAATGCCATTACCGGATGTAATGAGTGCAGTACTGGCTTTGGATGACACAGTTTTAGATATTGATCAGGTAGAAAACTTGATAAAGTTTTGCCCCACAAAAGAAGAAATGGATCTCCTCAA
GGGATATAATGGAGAGAGAGAGAATCTGGGCAAATGTGAGCAGTTTTTCTTGGAGATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGA
GAATTATGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACGGGGTTCTGCGATTGGCTTTAGGCTAGATAGTCTTCTGAAATTGATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAGGTTCTTGCTGAGAAACTTCCAGAGCTCCTTGATTTCAATAAGGATCTT
GCTCATTTGGAAGCTGCATCAAAGAGACAATTGAAGACATTAGCAGAGGAGATGCAAGCTGTAAGCAAAGGTATAGAAAAGGTTGAGCAGGAACTAACTGCATCAGAGAATGATGGTCCAGTATCTGAAGGGTTTCGGAAGCTATTGAAAGGTTTCCTTGTTGATGCTGAAGCTGATGTGAAATCTCTGACCTACCTCTA
TTCAGAAGTGGGTCGGAATGCAGATGCACTTGCTCTTTTTTTTGGGGAAGACCCTGCACGATGTCCATTTGAACAGGTGGTTTCTACTCTTCTCAACTTTGTGACAATGTTTCGGCGGGCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGATGAAACTTT
CTCCCTTGAGAATAGATGCTGGAGAACAGCCTTCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCTTTTTGGCTTATTGTAACACTAAAGTGCATATTCATTTTTGCCCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAATGACACAGAT
ATCATTTACCCACATGCCAATTGTGCTTGTAAAACTATCAAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTACTATAGGCTGAACAAGTATATGGGGCAACAGCAAAAATAAGGGCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAATTTTGATGTA
ATGATAGCACACCATTTGTTTCGGATATTCGATAAACTAATTGATCATTCCAGGAATGTGCTTGAAGAGATGCATTGTGTTTATTCAGTACTTCCCTCTTTGAGAATATCCTCAGATTCAGTTCTTATCTGCAGTCCTCAATTCTTCAACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTTACTCTTCCA
AGAATATATTTTTGCTGGAGAGAACAACAACAAAGGGTATACCGACTCTTCTTTTATGAGTGCCAGGCGAAGGGCTTATTCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTGGTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATAATTGTTAGA
TAAAATTTTAAGCTCCCTTGTGAAATAAGAAACACATTGGGTAAACTTGCCAAGTTGCCATGTTTATCAGCATTGTAAACTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATATTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTCTTTCTCTAG
GCATTGGCTGCAACAGGTAAATTTTTACATGATCAAGATGCATACTGTAGATAGTATAGAATTTGAACA

>TRINITY_DN47769_c0_g3_i1 from sample A7 / Example unspliced isoform:

ATTGGGTTTTGCATCAGGAATGTGCTTGAAGAGATGCATTGTGTTTATTCAGTACTTCCCTCTTTGAGAATATCCTCAGgcaagttattgagggagcatcattgataggctatttaatcactttatgacgtgggcttatttggtctcattatggtagacctattgatatacgaaagcattcagtgttacaaatttatttg
tttattttggattaaagaagaatgacaaactatgttttaatttattcttgtcctttgtttggacagATTCAGTTCTTATCTGCAGTCCTCAATTCTTCAACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTTACTCTTCCAAGAATATATTTTTGCTGGAGAGAACAACAACAAAGGGTATACCGACTCT
TCTTTTATGAGTGCCAGGCGAAGGGCTTATTCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTGGTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATAATTGTTAGATAAAATTTTAAGCTCCCTTACGAAATAAGAAACACATTGGGTAAACTTG
CCAAGTTGCCATGTTTATCAGCATTGTAAACTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATATTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTCTTTCTCTAGGCATTGGCTGCAACAGGTAAATTTTTACATGATCAAGATGCATACTGTA
GATAGTATAGAATTTGAACA

Intron 6 GTTAGTATAT ... ATGATTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	1	4	0
A2	62.96	5	12	5
A3	53.85	6	7	7
A4	100	0	1	0
A5	100	0	3	4
A6	-1	0	0	0
A7	-1	0	0	0
A8	100	0	2	0
A9	-1	0	0	0
A10	-1	0	0	0

>TRINITY_DN114463_c1_g1_i14 from sample A2 / Example spliced isoform

ACCAGATTAATGTTTTTTGAATGCGGGGTCATTGCAGTTTTTCTTGGAGATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTA
TGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACGGGGTAAGTTAAAGTGATTTCTACCTGGTTTGTTCCCCATCTTTGGAAATAATGTTTGTAGGTGGGAGAGGAATAAGAGAGAAAAAGTTCTGCGATTGGCTTTAGGCTAGATAGTCTTCTGAAATTGATAGATACACGTGCTCGCAACAACA
AGATGACTCTGATGCACTATCTTTGTAAG

>TRINITY_DN114463_c1_g1_i11 from sample A2 / Example unspliced isoform:

ACCAGATTAATGTTTTTTGAATGCGGGGTCATTGCAGTTTTTCTTGGAGATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTA
TGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACGGGGTAAGTTAAAGTGATTTCTACCTGGTTTGTTCCCCATCTTTGGAAATAATGTTTGTAGGTGGGAGAGGAATAAGAGAGAAAAAgttagtatattcaactattcttcaaatcatatatgaactaacctccaccttctgttcctttcctcc
atgtgtcattggagacaaattgaagtgctgggcaatatatattttggatttcctgtatttagaatattgcagccctattggttctctttaatctgattgttgctttacatacttctattactttaatcactatttttctcactgatttctatgattttagGTTCTGCGATTGGCTTTAGGCTAGATAGTCTTCTGAAATT
GATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAG

Intron 7 GTATAGAAAA ... GTTTCGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.38	10	39	38
A2	80.49	8	29	37
A3	80.39	5	21	20
A4	88.46	3	29	17
A5	75	3	10	8
A6	82.98	4	17	22
A7	75.82	11	38	31
A8	75.81	15	46	48
A9	71.43	17	36	49
A10	96.23	1	30	21

>TRINITY_DN114463_c1_g1_i6 from sample A2 / Example spliced isoform

GGAGATGCAAGCTGTAAGCAAAGCTATTGAAAGGTTTCCTTGTTGATGCTGAAGCTGATGTGAAATCTCTGACCTACCTCTATTCAGAAGTGGGTCGGAATGCAGATGCACTTGCTCTTTTTTTTGGGGAAGACCCTGCACGATGTCCATTTGAACAGGTGGTTTCTACTCTTCTCAACTTTGTGACAATGTTTCGGCGG
GCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGATGAAACTTTCTCCCTTGAGAATAGATGCTGGAGAACAGCCTTCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCTTTTTGGCTTATTGTAACACTAAAGTGCATATTCATTTTTGC
CCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAATGACACAGATATCGTTTACCAACATGCCAATTGTGCTTGTAAAACTATCGAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTACTATAGGCTGAACAAGTATATGGGGCAACAGCAAAAATAAGG
GCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAATTTTGATGTAATGATAGCACACCATTTGTTTCGGATATTCGATAAACTAATTGATCAT

>TRINITY_DN114463_c1_g1_i15 from sample A2 / Example unspliced isoform:

GTGATTTATTTTCTCTCATATCCAAAGAAACTATTGTAGTTGCACTTTCATGTTTAACTACTTCATCATTGTTATTTTGAGCTTTTGGAATTTCACAGGTTAGGGAATCTATCAAGCTACGGAGAATTATGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACGGGGTTCTGCGATTGGCTTTAGG
CTAGATAGTCTTCTGAAATTGATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAGGTTCTTGCTGAGAAACTTCCAGAGCTCCTTGATTTCAATAAGGATCTTGCTCATTTGGAAGCTGCATCAAAGAGACAATTGAAGACATTAGCAGAGGAGATGCAAGCTGTAAGCAAAGgtataga
aaaggttgagcaggaactaactgcatcagagaatgatggtccagtatctgaagggtttcggaagCTATTGAAAGGTTTCCTTGTTGATGCTGAAGCTGATGTGAAATCTCTGACCTACCTCTATTCAGAAGTGGGTCGGAATGCAGATGCACTTGCTCTTTTTTTTGGGGAAGACCCTGCACGATGTCCATTTGAACAGG
TGGTTTCTACTCTTCTCAACTTTGTGACAATGTTTCGGCGGGCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGATGAAACTTTCTCCCTTGAGAATAGATGCTGGAGAACAGCCTTCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCT
TTTTGGCTTATTGTAACACTAAAGTGCATATTCATTTTTGCCCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAATGACACAGATATCGTTTACCAACATGCCAATTGTGCTTGTAAAACTATCGAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTAC
TATAGGCTGAACAAGTATATGGGGCAACAGCAAAAATAAGGGCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAATTTTGATGTAATGATAGCACACCATTTGTTTCGGATATTCGATAAACTAATTGATCATCCCAGGAATGTGCTTGAAGAGATGCATTG
TGTTTATTCAGTACTTCCCTCTTTGAGAATATCCTCAGATTCAGTTCTTATCTGCAGTCCTCAATTCTTCAACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTTACTCTTCCAAGAATATATTTTTGCTGGAGAGAACAACAACAAAGGGTATACCGACTCTTCTTTTATGAGTGCCAGGCGAAGGGCTT
ATTCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTGGTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATAATTGTTAGATAAAATTTTAAGCTCCCTTACGAAATAAGAAACACATTGGGTAAACTTGCCAAGTTGCCATGTTTATCAGCATTGTA
AACTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATATTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTCTTTCTC

Intron 8 GTCTGTGGAC ... ATTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.71	65.9	9	24
A2	25.24	23.7	2	8
A3	13.51	32.1	0	5
A4	21.43	22	6	6
A5	40	6	1	4
A6	12.62	13.9	0	2
A7	19.25	29.4	0	7
A8	25.96	31.4	0	11
A9	14.13	30.7	0	5
A10	18.35	26.7	2	6

>TRINITY_DN126477_c2_g1_i19 from sample A1 / Example spliced isoform

ACAAGGATAGGAGTTCCACAACTTCAAATGTAAATTACATTGTTTTAGAAGACTTTTGTAATATTTATACAGGTTGAAAACTTGAAATCTTTTCTTCGTTAGTATTTGTGGTCTGACATTACAGGATACAATTTTCAGTTCAAAGCATTTGTATTATTTTAAGGTTATAAGAAAGTTTGTTATAATGGATGTGTGCAGGT
TGATTTAAGAAGGGCTAATAACTGCGAAATTATGCTTACTAAGGTCAAAATGCCATTACCGGATGTAATGAGTGCAGTACTGGCTTTGGATGACACAGTTTTAGATATTGATCAGGTAGAAAACTTGATAAAGTTTTGCCCCACAAAAGAAGAAATGGATCTCCTCAAGGGATATAATGGAGAGAGAGAGAATCTGGGCA
AATGTGAGCAGTTTTTCTTGGAGATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTATGCAAACGATTCTTTCTTTAGGAAAT
GCATTGAATCAGGGAACTGCACGGGGTTCTGCGATTGGCTTTAGGCTAGATAGTCTTCTGAAATTGATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAGGTTCTTGCTGAGAAACTTCCAGAGCTCCTTGATTTCAATAAGGATCTTGCTCATTTGGAAGCTGCATCAAAGAGACAATT
GAAGACATTAGCAGAGGAGATGCAAGCTGTAAGCAAAGGTATAGAAAAGGTTGAGCAGGAACTAACTGCATCAGAGAATGATGGTCCAGTATCTGAAGGGTTTCGGAAGCTATTGAAAGGTTTCCTTGTTGATGCTGAAGCTGATGTGAAATCTCTGACCTACCTCTATTCAGAAGTGGGTCGGAATGCAGATGCACTTG
CTCTTTTTTTTGGGGAAGACCCTGCACGATGTCCATTTGAACAGGTGGTTTCTACTCTTCTCAACTTTGTGACAATGTTTCGGCGGGCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGATGAAACTTTCTCCCTTGAGAATAGATGCTGGAGAACAGCCT
TCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCTTTTTGGCTTATTGTAACACTAAAGTGCATATTCATTTTTGCCCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAATGACACAGATATCATTTACCCACATGCCAATTGTGCTTGTAA
AACTATCAAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTACTATAGGCTGAACAAGTATATGGGGCAACAGCAAAAATAAGGGCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAATTTTGATGTAATGATAGCACACCATTTGTTTCGGATATTCGA
TAAACTAATTGATCATTCCAGGAATGTGCTTGAAGAGATGCATTGTGTTTATTCAGTACTTCCCTCTTTGAGAATATCCTCAGATTCAGTTCTTATCTGCAGTCCTCAATTCTTCAACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTTACTCTTCCAAGAATATATTTTTGCTGGAGAGAACAACAACA
AAGGGTATACCGACTCTTCTTTTATGAGTGCCAGGCGAAGGGCTTATTCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTGGTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATAATTGTTAGATAAAATTTTAAGCTCCCTTGTGAAATAAGAAA
CACATTGGGTAAACTTGCCAAGTTGCCATGTTTATCAGCATTGTAAACTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATATTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTCTTTCTCTAGGCATTGGCTGCAACAGGTAAATTTTTACATGA
TCAAGATGCATACTGTAGATAGTATAGAATTTGAACAGCATACCAAGCTTGTCAAAGAAATGAGAAATGGCAAGACTGAAACAATGGTGGAAGGCTGGAAAAGGGGGAAAATTT

>TRINITY_DN126477_c2_g1_i3 from sample A1 / Example unspliced isoform:

CATAACCAGATTAATGTTTTTTGAATGCGGGGTCATTGCAGTTTTTCTTGGAGATGATGAAAGTTCCAAGAGTGGAGTCTAAATTAAGAGTATTCTCCTTCAAGAGTCAGTTTATCTCTCAGgtctgtggacaatactcatttttcatttccagttcagctttcgtaagtgaaatagaagttctagacttatgtgagaaa
caccttgcactacaattaatgctgtttgttgttttattttttcagGTTTCTGACCTACGAAAAAGTCTAAACATTGTAAAGTATGCATCTCAGGAGGTTAGGGAATCTATCAAGCTACGGAGAATTATGCAAACGATTCTTTCTTTAGGAAATGCATTGAATCAGGGAACTGCACGGGGTTCTGCGATTGGCTTTAGGCT
AGATAGTCTTCTGAAATTGATAGATACACGTGCTCGCAACAACAAGATGACTCTGATGCACTATCTTTGTAAGGTTCTTGCTGAGAAACTTCCAGAGCTCCTTGATTTCAATAAGGATCTTGCTCATTTGGAAGCTGCATCAAAGAGACAATTGAAGACATTAGCAGAGGAGATGCAAGCTGTAAGCAAAGGTATAGAAA
AGGTTGAGCAGGAACTAACTGCATCAGAGAATGATGGTCCAGTATCTGAAGGGTTTCGGAAGCTATTGAAAGGTTTCCTTGTTGATGCTGAAGCTGATGTGAAATCTCTGACCTACCTCTATTCAGAAGTGGGTCGGAATGCAGATGCACTTGCTCTTTTTTTTGGGGAAGACCCTGCACGATGTCCATTTGAACAGGTG
GTTTCTACTCTTCTCAACTTTGTGACAATGTTTCGGCGGGCACATGAGGAGAACATCAAACAAGCTGAACTTGAAAGAAAGAAAGCAGAAAAGGAGGCAGAAAAGGAGAGGATGAAACTTTCTCCCTTGAGAATAGATGCTGGAGAACAGCCTTCATCTCCTCTCAGAAGGAAGTCGAAATAATAAAGATGAACACCTTT
TTGGCTTATTGTAACACTAAAGTGCATATTCATTTTTGCCCATGGTGCAGGAAAATGCCCTTTACTCATACAGTGCATGAGGTCATGCATTTATTGGAATGTTGGCAACAATGACACAGATATCATTTACCCACATGCCAATTGTGCTTGTAAAACTATCAAAATTTTATTTGCAAAATTGCAAAGTTCTGTATTTACTA
TAGGCTGAACAAGTATATGGGGCAACAGCAAAAATAAGGGCATCGTAAGTCAGGATTTTAATATTGGCTGATGTACAGATGTGCTTATGCGGTTTCTGGTGGTATGCAGAATTTTGATGTAATGATAGCACACCATTTGTTTCGGATATTCGATAAACTAATTGATCATCCCAGGAATGTGCTTGAAGAGATGCATTGTG
TTTATTCAGTACTTCCCTCTTTGAGAATATCCTCAGATTCAGTTCTTATCTGCAGTCCTCAATTCTTCAACCTCTGCTGGATGCTTCTCTATAAATTGTACTCAAATTATTTACTCTTCCAAGAATATATTTTTGCTGGAGAGAACAACAACAAAGGGTATACCGACTCTTCTTTTATGAGTGCCAGGCGAAGGGCTTAT
TCACCGGCAACCTGAGTTTGGATATTCATTGTTCTTAAGATCGGTAATTTTATTTTGTAAGATTTGTGGTAACATGATTTCAAATGCATTATTTGAAAGGGTGTATCTTATAATTGTTAGATAAAATTTTAAGCTCCCTTGTGAAATAAGAAACACATTGGGTAAACTTGCCAAGTTGCCATGTTTATCAGCATTGTAAA
CTAAGACTTCACAAATGAGAAAGATTTGAAGTTATCCACTTAGCTGGTTGTTGCGAAGCATCAGTATATTGAGTGGATATTTTTCCTTAGTCAGTTTCTTACGTCTTTGTTCTTTCTCTAGGCATTGGCTGCAACAGGTAAATTTTTACATGATCAAGATGCATACTGTAGATAGTATAGAATTTGAACAGCATACCAAG
CTTGTCAAAGAAATGAGAAATGGCAAGACTGAAACAATGGTGGAAGGCTGGAAAAGGGGGAAAATTT