sp|Q42538|SAT5_ARATH ----> Serine acetyltransferase 5

Intron 1 GTAAGGTTTA ... ATAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.79	21	35	18
A2	52.54	14	17	14
A3	42.86	22	27	6
A4	55.81	19	31	17
A5	46.67	4	5	2
A6	23.26	33	19	1
A7	18.28	76	27	7
A8	28	45	29	6
A9	25.65	71	39	10
A10	28.96	65	43	10

>TRINITY_DN111184_c1_g1_i7 from sample A4 / Example spliced isoform

TGGGGCCTGTTTAAAGATCTGATGGAGAGGGCCGAGAAGAAGATCGCCAGGACTTCAAAAATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGTTACCTCGATGCTTCCTGAGTAAAACTTCCTGCACTATCAGGGTTTCTTTTCCCTGGGTCGGTGTATGGTTATGACGAATACTCTCTCACATGC
ATATGATTTTGGGAGAGCTCTGGTCGACTTTGGTTTCGGTCTTCAACTATGGATGAACGCTAACCCGTTTCGGAGCTACATGATCTTTTAAAACCATGGATGTCCGGGTTGCCGAGGACATGGTATCCTTTTAACAAGTTACGACCTATATCGAATTTTTGAGTTATAGTCATGAATATTGATAGGTTGTCATCTTGATA
GACAAACCCACGAAGAGTGTAATGTGGTTGATTATTGTTGTGAGTAAAAGCTTAAATACTCATAGAAATA

>TRINITY_DN111184_c1_g1_i16 from sample A4 / Example unspliced isoform:

GTTGAATTTATGAATCGTTGTTGGGTTAGATTCAATTTCTGATAGGTTGAATTCCTGATGGGTTGAATTTCTGAATCGTTGTTGTATGATATGAATTTAACGGCGCGTTCGAATTTCAAACATCTGGAGGGACTACTTGATACGCTGGGTTTGATATTGATGAATCTGAAGAATCTGTACATTCAAATAAGATAACTTCT
CTTCCGCCATAGATCGCCTACATGAGATCTGATTTAACAGTCCTGAAGCCGAACGACGTGGGTGGCACTCAAGTACGTCTGTGACTAGTAATCGACGTGTCTGAACGGTGCCAGGACTTCAAAAATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGGAGTTGAATCCTGAGTTATATTTGGTTTCC
AGTTACCTCGATGCTTCCTGAGTTAAACTTCCTGCACTATCAGGTAAGAATCCTCTCTCCGAACTGCATTTTGTCTTATATATAAAAACCAACTATCAACATTTTCTCTTATTTCATTACCTTTCTTGAGCTGCCGAGTAGGGATTTTACCTGTAGGGTTTTGGGCTTGAGCTGCCCATTCGTGTTCTCTAACCAGGGTT
TCTTTTCCCTGGGTCGGTGTATGGTTATGACGAATACTCTCTCACATGCATATGATTTTGGGAGAGCTCTGGTCGACTTTGGTTTCGGTCTTCAACTATGGATGAACGCTAACCCGTTTCGGAGCTACATGATCTTTTAAAACCATGgtaaggtttacctacacacatttgttttagggcattaattttacaatatggtc
ttcatattccaacgatacttaactacatataataattgcagGATGTCCGGGTTGCCGAGGACATGGTATCCTTTTAACAAGTTACGACCTATATCGAATTTTTGAGTTATAGTCATGAATATTGATAGGTTGTCATCTTGATAGACAAACCCACGAAGAGTGTAATGTGGTTGATTATTGTTGTGAGTAAAAGCTTAAAT
ACTCATAGAAATATGGTTGAATAAGGGATAGGTATGGTCGTTTTTGTTCAATACTAATTTATGCACATTTTCACTAT

Intron 2 GTGAGTCTCA ... TGAATTTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.96	30	51	8
A2	61.16	47	74	10
A3	61.4	44	70	15
A4	64.21	34	61	8
A5	61.11	14	22	0
A6	35.96	57	32	12
A7	29.07	122	50	27
A8	36.69	88	51	11
A9	34.09	87	45	25
A10	35.8	104	58	18

>TRINITY_DN115810_c0_g1_i5 from sample A3 / Example spliced isoform

TCCTTTAATCGAATGGGATCCAAAAACGGCATTATTCATCGAAAAAGCCAACGGTGATTTATCAATCGAAAAATCCTTTAATCGAATGGGATCCAAGTGTGAGTGCAGTTTGAAATTCAAACAGTTAGGTTAAGGAAAACAGACTTGGCCGAGAAGATATTAGGGTCTCCGTGAATGCAGGCCAGGGGAGTGCTTCCTTA
CATAGAACCGACTTGGCCGATGGGGCCTGTTTAAAGATCTGATGGAGAGGGCCGAGAAGAAGATCGCCTCTGCTATATCTGCCCGAATGCGCCAGTGGCCTTGGCGCGTTCGAATTCCAACCATATGGAGGGACTGCTTGATGAATCTGAAGTATCTGAAGAATCAAATAAGATAACTTCTCTTCCGCCATAGATCGCCT
ACATGAGATCTGATTTAACCGTCCTGAAGCCGAATGACGTGGGTGACTCCCAAGTACGTCGGTGACTAGTAATCGAAGTGGCTGAACGGTGCGAGGAATTCAAATATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGGTATGTTTGTTTCTCTTCTGGTTTTCAATGTGGTAAAAAATGGTGTT

>TRINITY_DN115810_c0_g1_i4 from sample A3 / Example unspliced isoform:

TCCTTTAATCGAATGGGATCCAAAAACGGCATTATTCATCGAAAAAGCCAACGGTGATTTATCAATCGAAAAATCCTTTAATCGAATGGGATCCAAGTGTGAGTGCAGTTTGAAATTCAAACAGTTAGGTTAAGGAAAACAGACTTGGCCGAGAAGATATTAGGGTCTCCGTGAATGCAGGCCAGGGGAGTGCTTCCTTA
CATAGAACCGACTTGGCCGATGGGGCCTGTTTAAAGATCTGATGGAGAGGGCCGAGAAGAAGATCGCCTCTGCTATATCTGCCCGAATGCGCCAGTGGCCTTgtgagtctcagaagtcttgatgttgcttcaaatcacctcaaatctctttctgaagcttaatcgttactattcctgagagcatactaaattgccgctag
gtttatgtcccttcttatgttgaatttatgaatcgttgttgggttagattcaatttctgataggttgaattcctgatgggttgaattcctgtgagtctcagaagtcttgatgttgcttcaaatcacctcaaatctctttctgaagcttaatcgttactattcctgagagcatactcaattgccgctaggtttatatcctt
tcttatgttgaatttatgaatcgttgttgggttatagattcaatttctgaatcgttgttgaatttcttatgttgaatttatgaatcgttattggctttgtgtttataagatattaaattgctggtgatctttttaatgatctagagactggaaaatatatatcggtttggtcttggatccgttctcgtgaactatatgtc
ttttcttatgttgaatttatgaatcgttgttgggttatagattcaatttctgataggtttaatacctgctgggttgaatttctgaatcgttgttgtatgatatgaatttaagGGCGCGTTCGAATTCCAACCATATGGAGGGACTGCTTGATGAATCTGAAGTATCTGAAGAATCAAATAAGATAACTTCTCTTCCGCCA
TAGATCGCCTACATGAGATCTGATTTAACCGTCCTGAAGCCGAATGACGTGGGTGACTCCCAAGTACGTCGGTGACTAGTAATCGAAGTGGCTGAACGGTGCGAGGAATTCAAATATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGGATTGAATTCATATCAAATTTCCTTTGCATTATGTATAG
AAGTTGAATCCTGAGTTATATTTGGTTTCCAGTTACCTCGATGCTTCCTGAGTTAAACTTCCTGCACTATCA

Intron 3 GTAAGAATCC ... CTCTAACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.84	16	23.7	34
A2	65.29	12	17.3	27
A3	65.5	10.7	17.3	23
A4	68.26	10	14.3	29
A5	67.21	4.7	3.7	15.3
A6	51.96	18.3	13.3	25.8
A7	47.44	29.3	10.7	40
A8	42.51	26.3	7.7	27.3
A9	57.27	20.3	13.7	39.7
A10	51.95	32.7	19.7	46.8

>TRINITY_DN111184_c1_g1_i7 from sample A4 / Example spliced isoform

TGGGGCCTGTTTAAAGATCTGATGGAGAGGGCCGAGAAGAAGATCGCCAGGACTTCAAAAATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGTTACCTCGATGCTTCCTGAGTAAAACTTCCTGCACTATCAGGGTTTCTTTTCCCTGGGTCGGTGTATGGTTATGACGAATACTCTCTCACATGC
ATATGATTTTGGGAGAGCTCTGGTCGACTTTGGTTTCGGTCTTCAACTATGGATGAACGCTAACCCGTTTCGGAGCTACATGATCTTTTAAAACCATGGATGTCCGGGTTGCCGAGGACATGGTATCCTTTTAACAAGTTACGACCTATATCGAATTTTTGAGTTATAGTCATGAATATTGATAGGTTGTCATCTTGATA
GACAAACCCACGAAGAGTGTAATGTGGTTGATTATTGTTGTGAGTAAAAGCTTAAATACTCATAGAAATA

>TRINITY_DN111184_c1_g1_i16 from sample A4 / Example unspliced isoform:

GTTGAATTTATGAATCGTTGTTGGGTTAGATTCAATTTCTGATAGGTTGAATTCCTGATGGGTTGAATTTCTGAATCGTTGTTGTATGATATGAATTTAACGGCGCGTTCGAATTTCAAACATCTGGAGGGACTACTTGATACGCTGGGTTTGATATTGATGAATCTGAAGAATCTGTACATTCAAATAAGATAACTTCT
CTTCCGCCATAGATCGCCTACATGAGATCTGATTTAACAGTCCTGAAGCCGAACGACGTGGGTGGCACTCAAGTACGTCTGTGACTAGTAATCGACGTGTCTGAACGGTGCCAGGACTTCAAAAATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGGAGTTGAATCCTGAGTTATATTTGGTTTCC
AGTTACCTCGATGCTTCCTGAGTTAAACTTCCTGCACTATCAGgtaagaatcctctctccgaactgcattttgtcttatatataaaaaccaactatcaacattttctcttatttcattacctttcttgagctgccgagtagggattttacctgtagggttttgggcttgagctgcccattcgtgttctctaaccagGGTT
TCTTTTCCCTGGGTCGGTGTATGGTTATGACGAATACTCTCTCACATGCATATGATTTTGGGAGAGCTCTGGTCGACTTTGGTTTCGGTCTTCAACTATGGATGAACGCTAACCCGTTTCGGAGCTACATGATCTTTTAAAACCATGGTAAGGTTTACCTACACACATTTGTTTTAGGGCATTAATTTTACAATATGGTC
TTCATATTCCAACGATACTTAACTACATATAATAATTGCAGGATGTCCGGGTTGCCGAGGACATGGTATCCTTTTAACAAGTTACGACCTATATCGAATTTTTGAGTTATAGTCATGAATATTGATAGGTTGTCATCTTGATAGACAAACCCACGAAGAGTGTAATGTGGTTGATTATTGTTGTGAGTAAAAGCTTAAAT
ACTCATAGAAATATGGTTGAATAAGGGATAGGTATGGTCGTTTTTGTTCAATACTAATTTATGCACATTTTCACTAT