NA ----> NA

Intron 1 GCATGCATAT ... TTTGTGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	78.06	48.5	132.5	161.5
A2	76.81	31.5	85	96.5
A3	71.64	31	62	74.5
A4	79.88	36	112	132.5
A5	85.54	6.5	29	34.5
A6	74.55	43.5	101.5	117.5
A7	69.5	87	163	182
A8	66.03	86.5	135	153.5
A9	72.56	58	133	146.5
A10	75.28	49.5	118	143

>TRINITY_DN122233_c1_g1_i6 from sample A1 / Example spliced isoform

AGATGGTACACCCAACTCTCAAATTCCTGTATCGGTGTACTGTGGTTTACAATAGACTTCAAACAGAAGAAACTATAAATTCCTGTATCGGTGTACTGTGGTAGTGTGGTTTACAATAGACTGCAGACAGAAGAAACTATATATGTATATGTTGGTTGTGTGAAGTAGAGGCCAAATCGACAACAGATGTTGCAGTATTA
ATTGAGGTGTCAATGAAATTGCATAATATTCAGCATCATTTGAGTGGGAGTTTA

>TRINITY_DN122233_c1_g1_i10 from sample A1 / Example unspliced isoform:

AGATGGTACACCCAACTCTCAAATTCCTGTATCGGTGTACTGTGGTTTACAATAGACTTCAAACAGAAGAAACTATAAATTCCTGTATCGGTGTACTGTGGTAGTGTGGTTTACAATAGACTGCAGACAGAAGAAACTATATATGTATATGTTGGTTGTGTGAAGTAGAGgcatgcatattttgggttgtattttgtgaa
agGCCAAATCGACAACAGATGTTGCAGTATTAATTGAGGTGTCAATGAAATTGCATAATATTCAGCATCATTTGAGTGGGAGTTTAGGTTGTCACGTTGGCAATT

Intron 2 GTATGCGCAT ... GTTGATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.23	130	138	196
A2	50.15	84	79	90
A3	55.47	61	81	71
A4	40.27	112	78	73
A5	57.35	29	29	49
A6	47.4	101	89	93
A7	54.49	162	186	202
A8	59.58	134	212	183
A9	50.83	133	138	137
A10	49.35	117	111	117

>TRINITY_DN122233_c1_g1_i10 from sample A1 / Example spliced isoform

AGATGGTACACCCAACTCTCAAATTCCTGTATCGGTGTACTGTGGTTTACAATAGACTTCAAACAGAAGAAACTATAAATTCCTGTATCGGTGTACTGTGGTAGTGTGGTTTACAATAGACTGCAGACAGAAGAAACTATATATGTATATGTTGGTTGTGTGAAGTAGAGGCATGCATATTTTGGGTTGTATTTTGTGAA
AGGCCAAATCGACAACAGATGTTGCAGTATTAATTGAGGTGTCAATGAAATTGCATAATATTCAGCATCATTTGAGTGGGAGTTTAGGTTGTCACGTTGGCAATT

>TRINITY_DN122233_c1_g1_i1 from sample A1 / Example unspliced isoform:

AGATGGTACACCCAACTCTCAAATTCCTGTATCGGTGTACTGTGGTTTACAATAGACTTCAAACAGAAGAAACTATAAATTCCTGTATCGGTGTACTGTGGTAGTGTGGTTTACAATAGACTGCAGACAGAAGAAACTATATATGTATATGTTGGTTGTGTGAAGTAGAGgtatgcgcattgtactttttaatcacattt
gtaagtatcatagcttttgtcagatttccaattccctatatagatcctttgcttctggatcgttttaattgttgattcagGCATGCATATTTTGGGTTGTATTTTGTGAAAGGCCAAATCGACAACAGATGTTGCAGTATTAATTGAGGTGTCAATGAAATTGCATAATATTCAGCATCATTTGAGTGGGAGTTTA

Intron 3 GTATGCGCAT ... TTTGTGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	90.3	16	138	160
A2	86.05	12	79	69
A3	84.05	13	81	56
A4	86.5	11	78	63
A5	96.72	1	29	30
A6	83.92	16	89	78
A7	82.83	37	186	171
A8	83.29	36	212	147
A9	80.34	29	138	99
A10	84.68	19	111	99

>TRINITY_DN122233_c1_g1_i6 from sample A1 / Example spliced isoform

AGATGGTACACCCAACTCTCAAATTCCTGTATCGGTGTACTGTGGTTTACAATAGACTTCAAACAGAAGAAACTATAAATTCCTGTATCGGTGTACTGTGGTAGTGTGGTTTACAATAGACTGCAGACAGAAGAAACTATATATGTATATGTTGGTTGTGTGAAGTAGAGGCCAAATCGACAACAGATGTTGCAGTATTA
ATTGAGGTGTCAATGAAATTGCATAATATTCAGCATCATTTGAGTGGGAGTTTA

>TRINITY_DN122233_c1_g1_i1 from sample A1 / Example unspliced isoform:

AGATGGTACACCCAACTCTCAAATTCCTGTATCGGTGTACTGTGGTTTACAATAGACTTCAAACAGAAGAAACTATAAATTCCTGTATCGGTGTACTGTGGTAGTGTGGTTTACAATAGACTGCAGACAGAAGAAACTATATATGTATATGTTGGTTGTGTGAAGTAGAGgtatgcgcattgtactttttaatcacattt
gtaagtatcatagcttttgtcagatttccaattccctatatagatcctttgcttctggatcgttttaattgttgattcaggcatgcatattttgggttgtattttgtgaaagGCCAAATCGACAACAGATGTTGCAGTATTAATTGAGGTGTCAATGAAATTGCATAATATTCAGCATCATTTGAGTGGGAGTTTA

Intron 4 GTAAAGCAAA ... GTACACCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	99.01	3	300	12
A2	96.49	8	220	20
A3	98.56	3	205	16
A4	97.66	5	209	24
A5	100	0	139	15
A6	98.86	4	348	18
A7	98.46	9	577	44
A8	96.32	20	524	40
A9	96.69	12	351	28
A10	98.05	9	452	21

>TRINITY_DN46613_c0_g2_i2 from sample A10 / Example spliced isoform

CGGGAGCTATTGATTGAAAGGAAACGGAATTTAGGGTTTATGTAATATGGTCATCAGGGAGAGAATCGGGTATGCGAAAAAGGGTATGCTGTCGCTTCAAGTAGGACCATTTTGAAGCGCTCTCAAATTCCTGTATCAGTGTACTGTGGTTTACAATGGACTTCGAACAGAAGAAACTATATATGTATATGTTTTGTTCT
GTGTGAAGTAGAGGCGAGAGTGAGAAGATGGTTGGATGGGTGCATTTACTATCAGATTGGAGAGC

>TRINITY_DN46613_c0_g2_i9 from sample A10 / Example unspliced isoform:

TTCGGGGTTTGAATCTGATCAAATTTCTTGCAATAATTTGTAATTTTAGGTTTTCTTTTCTATTTTGAAGGTGTAATAGGTATGTGATCTACAGAGTGTGTTTTGATGCACGCAATATCTGCAGTATGCGAAAAAGGGTATGCTGTCGCTTCAAGTAGGACCATTTTGAAGCGgtaaagcaaataatctctctgctctga
tttggaactgaatccactgcaaaatagtttctaacagtgcttctgagccttttgatttttcttcctgttttttaattgtttcatggccgtcatcagatggtacacccagCTCTCAAATTCCTGTATCAGTGTACTGTGGTTTACAATGGACTTCGGGTGTATTGTGGTTTACAATAGACTGCAAACAGAAGAAACTATAT
ATGTATATGTTTTGTTCTGTGTGAAGTAGAGGTATGCGTATTGTACTTTTTAATCACATTTGTAAGTATCATAGCATTGTGGTTGTATTTTGTGAAAGTCCAAATCGACAACAGATGTAGCAGTATTATTTGAGGTGTCAATGAAATTGCAT