NA ----> NA

Intron 1 GTGAGAAAAG ... CATTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.12	203	251	141
A2	52.68	106	133	103
A3	47.31	93	91	76
A4	49.27	108	115.8	94
A5	55.17	52	74	54
A6	48.08	196	202	161
A7	49.36	267	300.5	220
A8	49.13	294	302	266
A9	50.49	216	254.5	186
A10	48.76	186	218	136

>TRINITY_DN110707_c4_g1_i14 from sample A5 / Example spliced isoform

CAGTATGTGAAAAGGGGTATGCTGTGGAGTCAAGTAGTATCAATTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAATATAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGCTGCTTTGAA
GTAGAGATGTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTC
TGGTCTTTCTTCAGGCGAGCGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAAT
GAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAA

>TRINITY_DN110707_c4_g1_i12 from sample A5 / Example unspliced isoform:

GTTTCATGGATCTGTCAGTACCTGGGAGCCTTTCAGATTTTTAAGAGTTTCATGGATCTGTCAGTTCTTGGGAGCCTTTCGGATTTTCTTCCTGTTTTTTAATTTGTTCATGGTCGTTATCAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTA
TGTGTATATGTTGCTGCTTTGAAGTAGAGGTATGCGTATTATACTTTTTGGTCACATTTGTAACTATCATAGCATTTGTTAGATTTCTCATTTCCCTATATAGATCCTTTGCTTCTAGCTCACCTTAATTGTCTAGGTAGGCTTGTTTATTTTGGGTTGTATTTGTGAAAGTTCAAATCTTCAACAGATGTTGCAGCATC
ATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAG
CgtgagaaaagacttcttttaaccaagaacttattatatatatatatcaaagtcttcctgaatctggtgtaatgaattgattgaggttggcatttattgttacctttggcttcatcttgtggcatggacttcaaaactttatagtggcttttctgttcattcttcagGCGAGCGTGAGAAGATGATGCTAGAGGTGCATT
ACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTT
ATTTTCTCTGGATATGGTCTTCGAA

Intron 2 GTATGCGTAT ... TCTTCAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.3	277.4	346	193
A2	44.41	227.8	238	126
A3	48.22	189	244	108
A4	49.69	180.2	208	148
A5	56.05	86.8	142	79
A6	45.94	197.8	205	131
A7	34.27	386.6	273	130
A8	32.08	388.6	226	141
A9	34.88	324	219	128
A10	33.9	305.2	217	96

>TRINITY_DN110707_c4_g1_i14 from sample A5 / Example spliced isoform

CAGTATGTGAAAAGGGGTATGCTGTGGAGTCAAGTAGTATCAATTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAATATAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGCTGCTTTGAA

GTAGAGATGTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTC
TGGTCTTTCTTCAGGCGAGCGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAAT
GAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAA

>TRINITY_DN110707_c4_g1_i12 from sample A5 / Example unspliced isoform:

GTTTCATGGATCTGTCAGTACCTGGGAGCCTTTCAGATTTTTAAGAGTTTCATGGATCTGTCAGTTCTTGGGAGCCTTTCGGATTTTCTTCCTGTTTTTTAATTTGTTCATGGTCGTTATCAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTA
TGTGTATATGTTGCTGCTTTGAAGTAGAGgtatgcgtattatactttttggtcacatttgtaactatcatagcatttgttagatttctcatttccctatatagatcctttgcttctagctcaccttaattgtctaggtaggcttgtttattttgggttgtatttgtgaaagttcaaatcttcaacagATGTTGCAGCATC
ATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAG
CGTGAGAAAAGACTTCTTTTAACCAAGAACTTATTATATATATATATCAAAGTCTTCCTGAATCTGGTGTAATGAATTGATTGAGGTTGGCATTTATTGTTACCTTTGGCTTCATCTTGTGGCATGGACTTCAAAACTTTATAGTGGCTTTTCTGTTCATTCTTCAGGCGAGCGTGAGAAGATGATGCTAGAGGTGCATT
ACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTT
ATTTTCTCTGGATATGGTCTTCGAA

Intron 3 GTTGGCATTT ... CATTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	54.99	106	118	141
A2	57.96	66	79	103
A3	59.17	49	66	76
A4	55.89	73	91	94
A5	56.67	39	48	54
A6	59.13	103	137	161
A7	58.72	148	201	220
A8	52.63	223	229.5	266
A9	59.7	116	157.8	186
A10	57.49	95	121	136

>TRINITY_DN110707_c4_g1_i1 from sample A5 / Example spliced isoform

GTTTCATGGATCTGTCAGTACCTGGGAGCCTTTCAGATTTTTAAGAGTTTCATGGATCTGTCAGTTCTTGGGAGCCTTTCGGATTTTCTTCCTGTTTTTTAATTTGTTCATGGTCGTTATCAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTA
TGTGTATATGTTGCTGCTTTGAAGTAGAGATGTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCT
TCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAGCGTGAGAAAAGACTTCTTTTAACCAAGAACTTATTATATATATATATCAAAGTCTTCCTGAATCTGGTGTAATGAATTGATTGAGGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAA
CATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAA

>TRINITY_DN110707_c4_g1_i13 from sample A5 / Example unspliced isoform:

CAGTATGTGAAAAGGGGTATGCTGTGGAGTCAAGTAGTATCAATTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAATATAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGCTGCTTTGAA
GTAGAGATGTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTC
TGGTCTTTCTTCAGGCGAGCGTGAGAAAAGACTTCTTTTAACCAAGAACTTATTATATATATATATCAAAGTCTTCCTGAATCTGGTGTAATGAATTGATTGAGgttggcatttattgttacctttggcttcatcttgtggcatggacttcaaaactttatagtggcttttctgttcattcttcagGCGAGCGTGAGAAG
ATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGG
GGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAA

Intron 4 GTATGCGTAT ... GAGATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.82	106	346	222
A2	66.33	101	238	160
A3	74.55	64	244	131
A4	75.1	62	208	166
A5	81.95	24	142	76
A6	70.46	74	205	148
A7	62.78	142	273	206
A8	57.81	166	226	229
A9	60.81	136	219	203
A10	59.59	117	217	128

>TRINITY_DN110707_c4_g1_i15 from sample A5 / Example spliced isoform

ATGGTACAACCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGCTGCTTTGAAGTAGAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTAT
CTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAGCGTGAGAAAAGACTTCTTTTAACCAAGAACTTATTATATATATATATCAAAGTCTTCCTGAATCTGGTGTAATGAATTGATTGAGGTTGGCATTTATTGTTACCTTTGGCTTCATCTTG
TGGCATGGACTTCAAAACTTTATAGTGGCTTTTCTGTTCATTCTTCAGGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTC
TTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAA

>TRINITY_DN110707_c4_g1_i12 from sample A5 / Example unspliced isoform:

GTTTCATGGATCTGTCAGTACCTGGGAGCCTTTCAGATTTTTAAGAGTTTCATGGATCTGTCAGTTCTTGGGAGCCTTTCGGATTTTCTTCCTGTTTTTTAATTTGTTCATGGTCGTTATCAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTA
TGTGTATATGTTGCTGCTTTGAAGTAGAGgtatgcgtattatactttttggtcacatttgtaactatcatagcatttgttagatttctcatttccctatatagatcctttgcttctagctcaccttaattgtctaggtaggcttgtttattttgggttgtatttgtgaaagttcaaatcttcaacagatgttgcagcatc
atatgcgttgtcaatgagattgcagACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAG
CGTGAGAAAAGACTTCTTTTAACCAAGAACTTATTATATATATATATCAAAGTCTTCCTGAATCTGGTGTAATGAATTGATTGAGGTTGGCATTTATTGTTACCTTTGGCTTCATCTTGTGGCATGGACTTCAAAACTTTATAGTGGCTTTTCTGTTCATTCTTCAGGCGAGCGTGAGAAGATGATGCTAGAGGTGCATT
ACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTT
ATTTTCTCTGGATATGGTCTTCGAA

Intron 5 GTTGTATGAT ... TCGTCATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.02	198.3	182	259.4
A2	52.72	156.4	168	176.7
A3	57.48	115.4	132.3	177.9
A4	56.94	107	123.9	155.1
A5	61.21	65.7	95.1	111.4
A6	50.88	99.3	68	133.1
A7	54.97	166.7	180.4	221.7
A8	53.3	146.4	136.7	190.3
A9	49.16	162	111	195.9
A10	53.49	121.3	133.6	140.7

>TRINITY_DN110707_c4_g1_i10 from sample A5 / Example spliced isoform

TTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAAAATAGATGGTACAACCAGCTCTCAAAATCCTCTACCGGTGTACTATGTTTTGCCGTAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGATACTTTGAATTAGAGGTATGCGTATTATACTTTTTGATCGCATTTGTAACTA
TCATAGCATTTGTTAGATTTCCATTTCCCTATATAGATCCTTTGCTTCTAGCTCACCTTATTCGCTCTATAGATCCTTTGCTTTTAGCTCACCTTAATTGTTGAGGTAGGCATGTTTATTTTGGGTTTTATTTGTGAAAGTCCAAATCTACCACAGATGTAGCAGCATCAAATGAGTTGTCAAATGAGATTGCAGACTAT
TTAGCATTAACTGAGTCGGAGATGAGGTTGTTATGTTGGCCACTGTTTGCAATTTCTTTGGACAGACTTCAAAGTGTCCTTGTGTATGATTTTTTGAAACTTAAGGGCGGCTCCTGGTCACCTTTGTTCGCCTCTGTACATGGCCTGTTAAGGCAGGCATCAGATGTTG

>TRINITY_DN110707_c4_g1_i16 from sample A5 / Example unspliced isoform:

TTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAAAATAGgttgtatgatctgccatatgtcatttaatggtccataagggttttattgatctgtcggtaattgcgagcctttcggatttaagggtttcatggatatgccagtacttgggagcctttggtttttcgtcctgttttttaattgc
ttcatggtcgtcatcagATGGTACAACCAGCTCTCAAAATCCTCTACCGGTGTACTATGTTTTGCCGTAGACTTCAAACAGAAGATACTCTGTATGTGTGTATGTTGATACTTTGAATTAGAGGTATGCGTATTATACTTTTTGATCGCATTTGTAACTATCATAGCATTTGTTAGATTTCCATTTCCCTATATAGATCC
TTTGCTTCTAGCTCACCTTATTCGCTCTATAGATCCTTTGCTTTTAGCTCACCTTAATTGTTGAGGTAGGCATGTTTATTTTGGGTTTTATTTGTGAAAGTCCAAATCTACCACAGATGTAGCAGCATCAAATGAGTTGTCAAATGAGATTGCAGACTATTTAGCATTAACTGAGTCGGAGATGAGGTTGTTATGTTGGC
CACTGTTTGCAATTTCTTTGGACAGACTTCAAAGTGTCCTTGTGTATGATTTTTTGAAACTTAAGGGCGGCTCCTGGTCACCTTTGTTCGCCTCTGTACATGGCCTGTTAAGGCAGGCATCAGATGTTG

Intron 6 GTGAGAAAAG ... ATTGATTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.36	203	231	106
A2	46.33	106	117	66
A3	43.12	93	92	49
A4	45.59	108	108	73
A5	50.71	52	68	39
A6	42.52	196	187	103
A7	43.61	267	265	148
A8	46.01	294	278	223
A9	44.25	216	227	116
A10	44.23	186	200	95

>TRINITY_DN110707_c4_g1_i14 from sample A5 / Example spliced isoform

CAGTATGTGAAAAGGGGTATGCTGTGGAGTCAAGTAGTATCAATTTGAAGCGGCAAAGCAAATAATCTCTCTGCGCTAAACTAAATCCACTGGAATATAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTATGTGTATATGTTGCTGCTTTGAA
GTAGAGATGTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCTTCAAGCGAGCGTCTGTCTCTTTC
TGGTCTTTCTTCAGGCGAGCGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAACATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAAT
GAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAA

>TRINITY_DN110707_c4_g1_i1 from sample A5 / Example unspliced isoform:

GTTTCATGGATCTGTCAGTACCTGGGAGCCTTTCAGATTTTTAAGAGTTTCATGGATCTGTCAGTTCTTGGGAGCCTTTCGGATTTTCTTCCTGTTTTTTAATTTGTTCATGGTCGTTATCAGATGGTACACCCAGCTCTCAAAATCCTCTACCGGTGTACTATGGTTTGCAATAGACTTCAAACAGAAGATACTCTGTA
TGTGTATATGTTGCTGCTTTGAAGTAGAGATGTTGCAGCATCATATGCGTTGTCAATGAGATTGCAGACTATTCAGCTTTTATCGAGTCGCGGTTGAGTTTGTCACGTTGGCCACTGTTTGCATTTTCCTTGGACATACTTCATAGTGGCCCTTATTTTATCTATCTGCATGACAATATGTGTCCCGTTTTGGTATTTCT
TCAAGCGAGCGTCTGTCTCTTTCTGGTCTTTCTTCAGGCGAGCgtgagaaaagacttcttttaaccaagaacttattatatatatatatcaaagtcttcctgaatctggtgtaatgaattgattgagGCGAGCGTGAGAAGATGATGCTAGAGGTGCATTACTGTCAGATTGCAGAGAGCATTACGAAGATGAAAAATAA
CATGAACACTGCAGGACTACTTCTACTAACTTCTACTTGTTACCAAAGCTTATTGTGATAGTAGATATATCAAAATGTCTTCCTCACCTTGCTGCAATGAATTGAATGAGTTGGCATTTCTTTTTACCTCTGGCTTCTTGGGGCCTTGACTATGGTCTTTATTTTCTCTGGATATGGTCTTCGAA