sp|Q9XH44|CET1_TOBAC ----> CEN-like protein 1

Intron 1 GTAAGCTTAG ... CTGTAACTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	13.12	135.5	22.4	5.3
A2	13.5	89.1	15.6	5.2
A3	11.67	163.4	23.4	7
A4	20.46	57.9	17	4.6
A5	12.44	77.4	11.8	5.8
A6	10.87	1176.1	155.6	34.6
A7	11.2	1080.8	146.8	47.1
A8	11.49	1204.9	170.5	41.6
A9	12.5	1320	206.8	43.2
A10	12.36	945.3	147.4	38.7

>TRINITY_DN124325_c1_g2_i3 from sample A1 / Example spliced isoform

TTTCAAAATCATCGATCTGAATTGGATGACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAGCGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGCCTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAGTTTTCACTACT
TTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATGGTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGGTATCACGGTTGTTTTAATTATTTTGCTGAGCTAGAAATCGGTCGAATGCGCGCCG
TAATGTTAACCGGGTTTTATGTTCATTTCGTTGTGCTGCAGTATTAGATATGCCA

>TRINITY_DN124325_c1_g2_i1 from sample A1 / Example unspliced isoform:

TTTCAAAATCATCGATCTGAATTGGATGACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAGCGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGCCTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAGTTTTCACTACT
TTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATGgtaagcttagattctatttattcattatttttgccataaactttttactatattggtcacattgaaattcacagtattaagcctttcttgaacctgtaactagGTTATGACGAATCCTGACGCTCCTGGCGGCCAGTA
TTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAG

Intron 2 GTATCACGGT ... TGTGCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.09	138.2	8.1	48.2
A2	26.13	108.4	4.8	39.7
A3	19.71	163.3	5.5	40.3
A4	23.08	70.3	2.8	21.5
A5	18.54	81.2	2.5	17.7
A6	21	1158.9	55.2	308.9
A7	23.37	1206	76	375
A8	23.88	1548	76.5	506.5
A9	21.55	1470	69.2	407.4
A10	23.6	974.6	65	311.5

>TRINITY_DN124325_c1_g2_i2 from sample A1 / Example spliced isoform

TTTCAAAATCATCGATCTGAATTGGATGACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAGCGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGCCTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAGTTTTCACTACT
TTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATGGTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGTATTAGATATGCCATGGGAGGAACTGCTTTCCTATGAAGCTCCAAGGGAGGGAACC
CGCAAGATAGTTTGTACAGCATTCAGAACGATTTTATCTCTGCCCAACACCAGTACCAGTACTAATACCAACATCGGTATGTTTGTGCCCAATATCATTATACCAAAGTCGCGGAACTATTTCAGTACCAGAGATTTTGCATCCACTAATGCACTTGGACCTCCGCTGGCTGCTTCTTTTTTCAGAATCAACAATCAGTC
T

>TRINITY_DN124325_c1_g2_i3 from sample A1 / Example unspliced isoform:

TTTCAAAATCATCGATCTGAATTGGATGACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAGCGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGCCTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAGTTTTCACTACT
TTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATGGTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGgtatcacggttgttttaattattttgctgagctagaaatcggtcgaatgcgcgccg
taatgttaaccgggttttatgttcatttcgttgtgctgcagTATTAGATATGCCA

Intron 3 GTATGCTATA ... CGTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	7.53	116	1.6	9.7
A2	5.64	82.6	3	5
A3	6.75	158.4	1.8	13
A4	9.91	59.2	2.6	6.9
A5	5.63	74.4	2.4	5
A6	7.43	1086.1	12.2	94.1
A7	9.11	1036.1	24.7	111.5
A8	9.37	1265.1	21.3	144.9
A9	7.44	1275.8	6	112.3
A10	11.77	843.4	27.2	119.6

>TRINITY_DN45296_c4_g1_i4 from sample A6 / Example spliced isoform

TCAGTACCAGAGATTTTGCATCCAGTAATGCACTTGGACCTCCGCTGGCAGCTTCCTTTTTCAAAATCATCGATCTGAATTGGATGAATTATTCCACAGAGATGTGTTACATTTAACACTAGCGACCTCCATCATTTCATGCTTGTTGATTTGGGATCTTCTGCAGACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAG
CGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGCCTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAGTTTTCACTACTTTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATG
GTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGTATTAGATATGCCATGGGAGGAACTGCTTTCCTATGAAGCTCCAAGGGAGGGAACCCGCAAGATAGTTTGTACAGCATTCAGAACGATTTTATCTCTGCCCAACACCAGTACCAGTAC
TAATACCAACATCGGTATGTTTGTGCCCAATATCATTATACCAAAGTCGCGGAACTATTTCAGTACCAGAGATTTTGCATACTTTAATGCATATGGACCTCCGCTGACTGCTGCTTTTTTCAGAATCATCGATCCGCTGGTTGCTGCTTTTTTCAGAATCGAGTATGACTGGAATGGTAATCAAAGATTTACCCGCATCT
AACGGCCATGTACGGTGAATGCCGTTTGAATATCTGTGAATTGGTTACACGTTTGATGACAAAAGCCAGAATCAAATTTTAACAGGCATGAAAATCTGAGCCGTCTTTATTATCATTTCAAGAGATTGTGAACATTTTAATCTGAAGATTTTGAAGGTTTCAAGAGTTTTCAACGTAATTTTATCTACCATTTCTTAACA
GAATTATTGCACCAGAAATGTTAACATGTAACACTGTAACG

>TRINITY_DN45296_c4_g1_i10 from sample A6 / Example unspliced isoform:

GACCCCACAGTTGTCACAATGGTAAGCTTAGATTCTATTTATTCATTATTTTTGCCATAAACTTTTTACTATATTGGTCACATTGAAATTCACATTATTAAGCCTTTCTTGAACCTGTAACTAGGTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGgtatgctataaacctcccgaagttaaatcaaa
ttaatgattgtaggacaccgaaacccattaaataactattcatgtcaacgttgtgcagGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGGTATCACGGTTGTTTTAATTATTTTGCTGAGCTAGAAATCGGTCGAATGCGCGCCGTAATGTTAACCGGGTTTTATGTTCATTTCGTTGTGCTGCAGTATTAGA
TATGCCATGGGAGGAACTGCTTTCCTATGAAGCTCCAAGGGAGGGAACCCGCAAGATAGTTTGTACAGCATTCAGAACGATTTTATCTCTGCCCAACACCAGTACCAGTACTAATACCAACATCGGTATGTTTGTGCCCAATATCATTATACCAAAGTCGCGGAACTATTTCAGTACCAGAGATTTTGCATACATTAATG
CATATGGACCTCCGCTGACTGCTGCTTTTTTCAGAATCATCGATCCGCTGGTTGCTGCTTTTTTCAGAATCGAGTATGACTGGAATGGTAATCAAAGATTTACCCGCATCTAACGGCCATATACGGTGAATGCTGTTTGAATAT

Intron 4 GTATGTTTGT ... CTTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.87	71	217	206
A2	70	70	177	149.8
A3	71.1	75	205	164
A4	74.5	32	103	84
A5	80.08	26	116	93
A6	71.51	762	1820	2004.8
A7	70.63	760	1861	1793.5
A8	69.65	1207	2488	3050.5
A9	69.37	996	2088	2423
A10	69.93	533	1307.8	1171

>TRINITY_DN48487_c1_g2_i6 from sample A10 / Example spliced isoform

GAGTATTTGCACTGGTATGCTATAAACCTCCCGAAGTTAAATCAAATTAATGATTGTAGGACACCGAAACCCATTAAATAACTATTCATGTCAACGTTGTGCAGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGGTATCACGGTTGTTTTAATTATTTTGCTGAGCTAGAAATCGGTCGAATGCGCGCCGTA
ATGTTAACCGGGTTTTATGTTCATTTCGTTGTGCTGCAGTATTAGATATGCCATGGGAGGAACTGCTTTCCTATGAAGCTCCAAGGGAGGGAACCCGCAAGATAGTTTGTACAGCATTCAGAACGATTTTATCTCTGCCCAACACCAGTACCAGTACTAATACCAACATCGAATCAACAATCAGTCTTAGAATGGTTATC
AAGAATTACACTCACGCACGTTGACTGCAGTTTGAATATCTGTGAACTGGCTACCCATGAATGTTTCAACCGTGTTTATTATCATTTGAATATCTGTGAACTGGCTACCCATGAATGTTTCAACCGTGTTTATTATCATTTGAATAGATTTTGAACGTTTTAGTCAGGAGGTTGTGAAGGTTTCCAAAGTTTCTTATCGT
AATTTTATCTGTGTTTCTTCATACAATTACTGCCCAGGGATGTGTCATATGTTATCTGTCATTTCTTAATGGAGTGATACTGTGGTTAATGTAATAATTTTATATTAAATATATTTATTTTTTAATTCAAGTTTTTTAATATAAAAA

>TRINITY_DN48487_c1_g2_i1 from sample A10 / Example unspliced isoform:

CTTCCTTTTTCAAAATCATCGATCTGAATTGGATGACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAGCGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGCCTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAGTTTT
CACTACTTTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATGGTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGTATTAGATATGCCATGGGAGGAACTGCTTTCCTATGAAGCTCCAAGGGA
GGGAACCCGCAAGATAGTTTGTACAGCATTCAGAACGATTTTATCTCTGCCCAACACCAGTACCAGTACTAATACCAACATCGgtatgtttgtgcccaatatcattataccaaagtcgcggaactatttcagtaccagagattttgcatccactaatgcacttggacctccgctggctgcttcttttttcagAATCAACA
ATCAGTCTTAGAATGGTTATCAAGAATTACACTCACGCACGTTGACTGCAGTTTGAATATCTGTGAACTGGCTACCCATGAATGTTTCAACCGTGTTTATTATCATTTGAATATCTGTGAACTGGCTACCCATGAATGTTTCAACCGTGTTTATTATCATTTGAATAGATTTTGAACGTTTTAGTCAGGAGGTTGTGAAG
GTTTCCAAAGTTTCTTATCGTAATTTTATCTGTGTTTCTTCATACAATTACTGCCCAGGGATGTGTCATATGTTATCTGTCATTTCTTAATGGAGTGATACTGTGGTTAATGTAATAATTTTATATTAAATATATTTATTTTTTAATTCAAGTTTTTTAATATAAAAA

Intron 5 GTAATCAAGA ... TCTTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.12	165	11	39
A2	22.81	88	13	26
A3	37.01	80	4	47
A4	26.06	105	16	37
A5	35.14	48	1	26
A6	31.55	525	11	242
A7	32.17	485	21	230
A8	32.19	455	14	216
A9	30.11	592	22	255
A10	35.38	453	18	248

>TRINITY_DN45296_c4_g1_i3 from sample A6 / Example spliced isoform

TCAGTACCAGAGATTTTGCATCCAGTAATGCACTTGGACCTCCGCTGGCAGCTTCCTTTTTCAAAATCATCGATCTGAATTGGATGACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAGCGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGC
CTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAGTTTTCACTACTTTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATGGTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACA
AGTATTAGATATGCCATGGGAGGAACTGCTTTCCTATGAAGCTCCAAGGGAGGGAACCCGCAAGATAGTTTGTACAGCATTCAGAACGATTTTATCTCTGCCCAACACCAGTACCAGTACTAATACCAACATCGGTATGTTTGTGCCCAATATCATTATACCAAAGTCGCGGAACTATTTCAGTACCAGAGATTTTGCAT
ACTTTAATGCATATGGACCTCCGCTGACTGCTGCTTTTTTCAGAATCATCGATCCGCTGGTTGCTGCTTTTTTCAGAATCGAGTATGACTGGAATGGTAATCAAAGATTTACCCGCATCTAACGGCCATGTACGGTGAATGCCGTTTGAATATCTGTGAATTGGTTACACGTTTGATGACAAAAGCCAGAATCAAATTTT
AACAGGCATGAAAATCTGAGCCGTCTTTATTATCATTTCAAGAGATTGTGAACATTTTAATCTGAAGATTTTGAAGGTTTCAAGAGTTTTCAACGTAATTTTATCTACCATTTCTTAACAGAATTATTGCACCAGAAATGTTAACATGTAACACTGTAACG

>TRINITY_DN45296_c4_g1_i6 from sample A6 / Example unspliced isoform:

TCAGTACCAGAGATTTTGCATCCAGTAATGCACTTGGACCTCCGCTGGCAGCTTCCTTTTTCAAAATCATCGATCTGAATTGGATGgtaatcaagaatcccccaagtgtaacggcggtgtatgttgagtcccgtttaaatatctgtgaactggctgcacgttttatgacaaaaacccgaatagaattttcgcagccatga
aagtttaagccgtgcttattatcatttcaatagattttaaacgttttaatgaggaggtgtgaaggttttcggagtttctcaaagtaattttatctgtaatttcttaatagaaatattccacagagatgtgttacatatatgtctgtgatttcttaacagaattattccacagagatgtgttacatttaacactagcgacc
tccatcatttcatgcttgttgatttgggatcttctgcagACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAGCGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGCCTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAG
TTTTCACTACTTTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATGGTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGTATTAGATATGCCATGGGAGGAACTGCTTTCCTATGAAGCTCCAA
GGGAGGGAACCCGCAAGATAGTTTGTACAGCATTCAGAACGATTTTATCTCTGCCCAACACCAGTACCAGTACTAATACCAACATCGGTATGTTTGTGCCCAATATCATTATACCAAAGTCGCGGAACTATTTCAGTACCAGAGATTTTGCATACTTTAATGCATATGGACCTCCGCTGACTGCTGCTTTTTTCAGAATC
ATCGATCCGCTGGTTGCTGCTTTTTTCAGAATCGAGTATGACTGGAATGGTAATCAAAGATTTACCCGCATCTAACGGCCATGTACGGTGAATGCCGTTTGAATATCTGTGAATTGGTTACACGTTTGATGACAAAAGCCAGAATCAAATTTTAACAGGCATGAAAATCTGAGCCGTCTTTATTATCATTTCAAGAGATT
GTGAACATTTTAATCTGAAGATTTTGAAGGTTTCAAGAGTTTTCAACGTAATTTTATCTACCATTTCTTAACAGAATTATTGCACCAGAAATGTTAACATGTAACACTGTAACG

Intron 6 GTAATCAAGA ... TTCTTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.16	7	11	12
A2	63.27	9	13	18
A3	56.52	5	4	9
A4	63.27	9	16	15
A5	75	1	1	5
A6	50	22	11	33
A7	56.52	25	21	44
A8	47.46	31	14	42
A9	57.41	23	22	40
A10	64.38	13	18	29

>TRINITY_DN45296_c4_g1_i4 from sample A6 / Example spliced isoform

TCAGTACCAGAGATTTTGCATCCAGTAATGCACTTGGACCTCCGCTGGCAGCTTCCTTTTTCAAAATCATCGATCTGAATTGGATGAATTATTCCACAGAGATGTGTTACATTTAACACTAGCGACCTCCATCATTTCATGCTTGTTGATTTGGGATCTTCTGCAGACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAG
CGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGCCTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAGTTTTCACTACTTTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATG
GTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGTATTAGATATGCCATGGGAGGAACTGCTTTCCTATGAAGCTCCAAGGGAGGGAACCCGCAAGATAGTTTGTACAGCATTCAGAACGATTTTATCTCTGCCCAACACCAGTACCAGTAC
TAATACCAACATCGGTATGTTTGTGCCCAATATCATTATACCAAAGTCGCGGAACTATTTCAGTACCAGAGATTTTGCATACTTTAATGCATATGGACCTCCGCTGACTGCTGCTTTTTTCAGAATCATCGATCCGCTGGTTGCTGCTTTTTTCAGAATCGAGTATGACTGGAATGGTAATCAAAGATTTACCCGCATCT
AACGGCCATGTACGGTGAATGCCGTTTGAATATCTGTGAATTGGTTACACGTTTGATGACAAAAGCCAGAATCAAATTTTAACAGGCATGAAAATCTGAGCCGTCTTTATTATCATTTCAAGAGATTGTGAACATTTTAATCTGAAGATTTTGAAGGTTTCAAGAGTTTTCAACGTAATTTTATCTACCATTTCTTAACA
GAATTATTGCACCAGAAATGTTAACATGTAACACTGTAACG

>TRINITY_DN45296_c4_g1_i6 from sample A6 / Example unspliced isoform:

TCAGTACCAGAGATTTTGCATCCAGTAATGCACTTGGACCTCCGCTGGCAGCTTCCTTTTTCAAAATCATCGATCTGAATTGGATGgtaatcaagaatcccccaagtgtaacggcggtgtatgttgagtcccgtttaaatatctgtgaactggctgcacgttttatgacaaaaacccgaatagaattttcgcagccatga
aagtttaagccgtgcttattatcatttcaatagattttaaacgttttaatgaggaggtgtgaaggttttcggagtttctcaaagtaattttatctgtaatttcttaatagaaatattccacagagatgtgttacatatatgtctgtgatttcttaacagAATTATTCCACAGAGATGTGTTACATTTAACACTAGCGACC
TCCATCATTTCATGCTTGTTGATTTGGGATCTTCTGCAGACAATGGAGGTGGCGAAGCAGTTGCTTATAGAAGCGGGAGTTATCGGAGGCATTGTGGGCGATTTTGATCCTCAATTTCATTTGGATGTAATCTACGGCCATAGACGAATATGCCTAGGTGATGAAGTCTTCCCTGCTCAAATGAATCAAAGACCTAGTAG
TTTTCACTACTTTCACGTTCCTGCTTATCCCTGGCGGCTGGAAACCTCCAGGGACCCCACAGTTGTCACAATGGTTATGACGAATCCTGACGCTCCTGGCGGCCAGTATTTGCACTGGTTATGTAGGCAATTCATCGGCGCCGAACAAGAACAAGTATTAGATATGCCATGGGAGGAACTGCTTTCCTATGAAGCTCCAA
GGGAGGGAACCCGCAAGATAGTTTGTACAGCATTCAGAACGATTTTATCTCTGCCCAACACCAGTACCAGTACTAATACCAACATCGGTATGTTTGTGCCCAATATCATTATACCAAAGTCGCGGAACTATTTCAGTACCAGAGATTTTGCATACTTTAATGCATATGGACCTCCGCTGACTGCTGCTTTTTTCAGAATC
ATCGATCCGCTGGTTGCTGCTTTTTTCAGAATCGAGTATGACTGGAATGGTAATCAAAGATTTACCCGCATCTAACGGCCATGTACGGTGAATGCCGTTTGAATATCTGTGAATTGGTTACACGTTTGATGACAAAAGCCAGAATCAAATTTTAACAGGCATGAAAATCTGAGCCGTCTTTATTATCATTTCAAGAGATT
GTGAACATTTTAATCTGAAGATTTTGAAGGTTTCAAGAGTTTTCAACGTAATTTTATCTACCATTTCTTAACAGAATTATTGCACCAGAAATGTTAACATGTAACACTGTAACG