NA ----> NA

Intron 1 GTAAAACACG ... TCGTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.17	7	3.7	3.9
A2	0	19.1	4.5	0
A3	69.05	1.8	4.8	4.8
A4	57	6	0	8.6
A5	58.67	2.1	0.9	3
A6	42.99	52	40.6	40.1
A7	47.65	52.2	35	48.8
A8	52.75	48.7	42.5	56.4
A9	45.43	49.8	36.3	42.9
A10	56.93	18.9	16.4	25.3

>TRINITY_DN129823_c9_g3_i7 from sample A1 / Example spliced isoform

GTATACAAGTTCAAGGATAGAAGGTAAAACACCCAAGAGATATGGTTTTGCCTTTCAACTTAGGCACACAATCCGTTTGATATTTTTTCCTGTATCCCGATTGGGAATTTTTAAACCAATGTTGTTGGTAGTGATCTATTGCCCTGTCTGTTCATCGTTTGTACTATTTTTATGAATTTTCCGCACAAGACAGGCATGTT
GCTTTAGAAGCTGCTGTTGCTCGTGGGAAGGTATTTGTTGCAGGAGCATCAGCTCCACGATCTAAGTGGGAGGAGGATGGTAGTAAGCTGCGTTCAGCGGCAATATCATTTTCACTTGTATAAATCTCAAGTTTGAGACACTGTAGTATAAGCCGTTCAAGCAAACTTGGAGTATACAAGTTCAAGGATAGAAGCTTCTA

CATGAGGCAAAGTTGTGTTGCACTTGAGAAAGAATGCATGACTGTTGACCTGAATCTCTCACGGTCCTTAGAAGGAGAAGACTGATTGTAAACTAGAAATGGGGAGCAACATTGTTTGCTGCTCGGACAATAAAAGTTAAGGACCCAATAATTCCGAGGACATACTATTTTTATGAATTTTCCGCACAAGACAGGCATGT
TGCTTTAGAAGCTGCTGTTGCTCGTGGGAAGGTATTTGTTGCAGGAGCATCAGCTCCACGATCTAAGTGGGAGGAGGATGGTAGTAAGCTGCGTTCAGCGGCAATATCATTTTCACTTGTATAAATCTCGAGTTTAAGACACTGTAGTATAAGCCGTTCAAGCAAACTTGGAGTATACAAGTTCAAGGATAGAAGGTAAA
ACACCCAAGAGATATGGTTTTGCCTTTCAACTTAGGCACACAATCCGTTTGATATTTTTTCCTGTATCCCGATTGGGAATTTTTAAACCAATGTTGTTGGTAGTGATCTATTGCCCTGTCTGTTCATCGTTTGTAGCTTCTACATGAGGCAAAGTTG

>TRINITY_DN129823_c9_g3_i7 from sample A1 / Example unspliced isoform:

GTATACAAGTTCAAGGATAGAAGGTAAAACACCCAAGAGATATGGTTTTGCCTTTCAACTTAGGCACACAATCCGTTTGATATTTTTTCCTGTATCCCGATTGGGAATTTTTAAACCAATGTTGTTGGTAGTGATCTATTGCCCTGTCTGTTCATCGTTTGTACTATTTTTATGAATTTTCCGCACAAGACAGGCATGTT
GCTTTAGAAGCTGCTGTTGCTCGTGGGAAGGTATTTGTTGCAGGAGCATCAGCTCCACGATCTAAGTGGGAGGAGGATGGTAGTAAGCTGCGTTCAGCGGCAATATCATTTTCACTTGTATAAATCTCAAGTTTGAGACACTGTAGTATAAGCCGTTCAAGCAAACTTGGAGTATACAAGTTCAAGGATAGAAGCTTCTA
CATGAGGCAAAGTTGTGTTGCACTTGAGAAAGAATGCATGACTGTTGACCTGAATCTCTCACGGTCCTTAGAAGGAGAAGACTGATTGTAAACTAGAAATGGGGAGCAACATTGTTTGCTGCTCGGACAATAAAAGTTAAGGACCCAATAATTCCGAGGACATACTATTTTTATGAATTTTCCGCACAAGACAGGCATGT
TGCTTTAGAAGCTGCTGTTGCTCGTGGGAAGGTATTTGTTGCAGGAGCATCAGCTCCACGATCTAAGTGGGAGGAGGATGGTAGTAAGCTGCGTTCAGCGGCAATATCATTTTCACTTGTATAAATCTCGAGTTTAAGACACTGTAGTATAAGCCGTTCAAGCAAACTTGGAGTATACAAGTTCAAGGATAGAAGgtaaa
acacccaagagatatggttttgcctttcaacttaggcacacaatccgtttgatattttttcctgtatcccgattgggaatttttaaaccaatgttgttggtagtgatctattgccctgtctgttcatcgtttgtagCTTCTACATGAGGCAAAGTTG

Intron 2 GTGGGTATCT ... CTGTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	63.21	14.8	27	24
A2	48.15	18.8	19	16
A3	55.31	22.8	25.9	30.5
A4	60.06	9.7	14	15
A5	74.36	5	13	16
A6	64.32	24.7	41.9	47
A7	62.95	29.8	52.7	48.3
A8	68.52	20.2	34	54
A9	62.69	32.7	48.8	61
A10	60.16	37.1	45.8	66

>TRINITY_DN129823_c9_g3_i3 from sample A1 / Example spliced isoform

GAAATCTCATTCATTTGAAAAAAACGAAACAACCTCCCCTCCATCATTCTCGCTACTCCCTATCCTTCCTCCATAAGGATAAAACTCAAGCTCCCATCCTTACAATTAAAAGAAACTGTGAGACAGCTCTGGTCCCAATCCTTCAGATACAAACACGCGCCCAAACATTATGATACTTCCGATGGCCAGCTGAAGGTCAG
TAGTAAGAGAATTATGGGTTTGGGTGACTGAGCAAAACTGTACCATATCCATTGGAGGAAGAAGAAGAATAATGCATCTCTCACTTCTCCATCATGGGCAAGATTGAACCAGAACTGAGAATTGTAGCTAATTTAACCACACAGAGCACCATGGAAGACAACAGCTGGGGAGATGATTGGGATAAAATGGTAGTAAAACA
CTCATTACTAGCCATTTCCTCAGAATTTGAGGACATGGGTTCCATGGGTGATGCTTTGTTCTGTTCAAAGAGACAGTTGATAATGGCGGCCCTGATGTTTGCATTATGTTCAGCAAACAATCTCTCAATTACTGCCGAAGCTGCAGAAGACAATGATGGGGATTCGGCAAAGCAGTCTGCTACTTCTAAGATTGTTTCTG
ATACTCCAAAGCAATCTGTGTCGAATAAAAGCATTTTTTCTGGCCTTTTAAATACACAGTTATGGTCGCAGTTCCGAGGAGATGGGTTCTCTCTGCGCGTCCCCCCAGATTTTGATGATCTCACAGAGCCCGAGGTAATTGTCTTGCTAAGCATAATATCATTCCTGAATCACCATGTTTGTGTTCCTATTCTTTCATCA
CCATTTTTTGATTTTGAGTTGGTGAAGCTAGGGCTCAAATGTAACTCCATGCTGATTAAGCACTTACATCTCAAGTCTCACAATGGCTTGGAAGGTCCCTAACAAGGTCCTGGTCAACCTAGTAAAGTACTAATCAACCTTCACTTGACAAAGGCA

>TRINITY_DN129823_c9_g3_i8 from sample A1 / Example unspliced isoform:

GAAATCTCATTCATTTGAAAAAAACGAAACAACCTCCCCTCCATCATTCTCGCTACTCCCTATCCTTCCTCCATAAGGATAAAACTCAAGCTCCCATCCTTACAATTAAAAGAAACTGTGAGACAGCTCTGGTCCCAATCCTTCAGATACAAACACGCGCCCAAACATTATGATACTTCCGATGGCCAGCTGAAGGTCAG
TAGTAAGAGAATTATGGGTTTGGGTGACTGAGCAAAACTGTACCATATCCATTGGAGGAAGAAGAAGAATAATGgtgggtatcttgattttaagccaacccacattggcctttctcagaaatactccccgtgatagaagcaacaagcttactaagtatgcaaatgggttttctgtttacagCATCTCTCACTTCTCCATC
ATGGGCAAGATTGAACCAGAACTGAGAATTGTAGCTAATTTAACCACACAGAGCACCATGGAAGACAACAGCTGGGGAGATGATTGGGATAAAATGGTAGTAAAACACTCATTACTAGCCATTTCCTCAGAATTTGAGGACATGGGTTCCATGGGTGATGCTTTGTTCTGTTCAAAGAGACAGTTGATAATGGCGGCCCT
GATGTTTGCATTATGTTCAGCAAACAATCTCTCAATTACTGCCGAAGCTGCAGAAGACAATGATGGGGATTCGGCAAAGCAGTCTGCTACTTCTAAGATTGTTTCTGATACTCCAAAGCAATCTGTGTCGAATAAAAGCATTTTTTCTGGCCTTTTAAATACACAGTTATGGTCGCAGTTCCGAGGAGATGGGTTCTCTC
TGCGCGTCCCCCCAGATTTTGATGATCTCACAGAGCCCGAGGATTACTCAGCAGGTGCATCATTATATGGAGAACGAGCAAAGCCCAAGACTTTTGCTGCACGTTTTGCATCATCTGATGGGTCAGAAGTATTGAGCGTGGTGATCCGACCTTCCACTGATCTCAAGTTGACTTTTTTGGAGGCCAAGGACATCTCAGAT
TTGGGTTCTTTAAGGGATGCTGCCAGCCTTTTTGTTCCAGGGGGAGCAACATTGTTTGCTGCTCGGACAATAAAAGTTAAGGACCCAATAATTCCGAGGACATACTATTTTTATGAATTTTCCGCACAAGACAGGCATGTTGCTTTAGAAGCTGCTGTTGCTCGTGGGAAGGTATTTGTTGCAGGAGCATCAGCTCCACG
ATCTAAGTGGGAGGAGGATGGTAGTAAGCTGCGTTCAGCGGCAATATCATTTTCACTTGTATAAATCTCAAGTTTGAGACACTGTAGTATAAGCCGTTCAAGCAAACTTGGAGTATACAAGTTCAAGGATAGAAGCTTCTACATGAGGCAAAGTTGTGTTGCACTTGAGAAAGAATGCATGACTGTTGACCTGAATCTCT
CACGGTCCTTAGAAGGAGAAGACTGATTGTAAACTAGAAATGGGGAGCAACATTGTTTGCTGCTCGGACAATAAAAGTTAAGGACCCAATAATTCCGAGGACATACTATTTTTATGAATTTTCCGCACAAGACAGGCATGTTGCTTTAGAAGCTGCTGTTGCTCGTGGGAAGGTATTTGTTGCAGGAGCATCAGCTCCAC
GATCTAAGTGGGAGGAGGATGGTAGTAAGCTGCGTTCAGCGGCAATATCATTTTCACTTGTATAAATCTCAAGTTTGAGACACTGTAGTATAAGCCGTTCAAGCAAACTTGGAGTATACAAGTTCAAGGATAGAAGGTAAAACACCCAAGAGATATGGTTTTGCCTTTCAACTTAGGCACACAATCCGTTTGATATTTTT
TCCTGTATCCCGATTGGGAATTTTTAAACCAATGTTGTTGGTAGTGATCTATTGCCCTGTCTGTTCATCGTTTGTAGCTTCTACATGAGGCAAAGTTG

Intron 3 GTCAGAAGTA ... TAATTCCGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.57	6	9	16
A2	65.22	8	18	12
A3	92.45	1	6	18.5
A4	56	11	17	11
A5	73.33	2	4	7
A6	59.39	40	66	51
A7	57.89	44	70	51
A8	74.68	20	58	60
A9	52.94	56	73	53
A10	71.11	13	31	33

>TRINITY_DN44176_c0_g2_i1 from sample A10 / Example spliced isoform

CTCCATCATTCTCGCTACTCCCTATCCTTCCTCCATAAGGATAAAACTCAAGCTCCCATCCTTACAATTAAAAGAAACTGTGAGACAGCTCTGGTCCCAATCCTTCAGATACAAACACGCGCCCAAACATTATGATACTTCCGATGGCCAGCTGAAGGTCAGTAGTAAGAGAATTATGGGTTTGGGTGACTGAGCAAAAC
TGTACCATATCCATTGGAGGAAGAAGAAGAATAATGGTGGGTATCTTGATTTTAAGCCAACCCACATTGGCCTTTCTCAGAAATACTCCCCGTGATAGAAGCAACAAGCTTACTAAGTATGCAAATGGGTTTTCTGTTTACAGCATCTCTCACTTCTCCATCATGGGCAAGATTGAACCAGAACTGAGAATTGTAGCTAA
TTTAGCCACACAGAGCACCATGAAAGACAACAGCTGGGGAGATGATTGGGATAAAATGGTAGTAAAACACTCATTACTAGCCATTTCCTCAGAATTTGAGGACATGGGTTCCATGGGTGATGCTTTGTTCTGTTCAAAGAGACAGTTGATAATGGCGGCCCTGATGTTTGCATTATGTTCAGCAAACAATCTCTCAATTA
CTGCCGAAGCTGCAGAAGACAATGATGGGGATTCGGCAAAGCAGTCTGCTACTTCTAACATTGTTTCTGATACTCCAAAGCAATCTGTGTCGAATAAAAGCATTTTTTCTGGCCTTTTAAATACACAGTTATGGTCGCAGTTCCGAGGAGATGGGTTCTCTCTGCGCGTCCCCCCAGATTTTGATGATCTCACAGAGCCC
GAGGATTACTCAGCAGGTGCATCATTATATGGAGAACGAGCAAAGCCCAAGACTTTTGCTGCACGTTTTGCATCATCTGATGGGACATACTATTTTTATGAATTTTCCGCACAAGACAGGCATGTTGCTTTAGAAGCTGCTGTTGCTCGTGGGAAGGAGCATCAGCTCCACGATCTAAGTGGGAGGAGGATGGTAGTAAG
CTGCGTTCAGCGGCAATATCATTTTCACTTGTATAAATCTCAAGTTTGAGACACTGTAGTATAAGCCGTTCAAGCAAACTTGGAGTATACAAGTTCAAGGATAGAAGCTTCTACATGAGGCAAAGTTGTGTTGCACTTGAGAAAGAATGCATGACTGTTGACCTGAATCTCTCACGGTCCTTAGAAGGAGAAGACTGATT
GTAAACTAGAAATGTAAGTTTGAGAAATTTTTCAAGTTCTTAAACAGTGCATTTGATGCAATATGTTTTTAATGAATAAAGATTTATAGTGAAGCTCCTCTAAAGGGGAAGCTATCTTGTATTATTAGATGCTTAGAATACTTTTTGTCTACATATCATCCGTTATTGGATTCAGTTCCAGCTCCCACTTCAAATAAAGC
TCTATTTGATTTTAAATTGGCAAAAATACTGGTATGTTTGAGTGTCAACTTGTGATGTAGCTTGTCTGTTTCATCTTTTAAGAGATGAAGCTTGAAAGACTATTGTTATGGTTTTCGAGTTGTCTGGGCTTAGATTTCTTGTTCTTTTAACCTCTCATCATAAACTTGGTGTGTTCCTTCCATCCTGTGTTTTTGGCTTA
TGATTAATAAACCTACCCTTTGAAGAATCTTGTGAGAATGTTAGGATGAAGATGGAGCTAAACCATTGGAATTTTATATTGGTTGATCTGCTCTATTGTTAGTAATACATGAGCC

>TRINITY_DN44176_c0_g2_i5 from sample A10 / Example unspliced isoform:

CTCCATCATTCTCGCTACTCCCTATCCTTCCTCCATAAGGATAAAACTCAAGCTCCCATCCTTACAATTAAAAGAAACTGTGAGACAGCTCTGGTCCCAATCCTTCAGATACAAACACGCGCCCAAACATTATGATACTTCCGATGGCCAGCTGAAGGTCAGTAGTAAGAGAATTATGGGTTTGGGTGACTGAGCAAAAC
TGTACCATATCCATTGCAGGAAGAAGAAGAATAATGCATCTCTCACTTCTCCATCATGGGCAAGATTGAACCAGAACTGAGAATTGTAGCTAATTTAGCCACACAGAGCACCATGAAAGACAACAGCTGGGGAGATGATTGGGATAAAATGGTAGTAAAACACTCATTACTAGCCATTTCCTCAGAATTTGAGGACATGG
GTTCCATGGGTGATGCTTTGTTCTGTTCAAAGAGACAGTTGATAATGGCGGCCCTGATGTTTGCATTATGTTCAGCAAACAATCTCTCAATTACTGCCGAAGCTGCAGAAGACAATGATGGGGATTCGGCAAAGCAGTCTGCTACTTCTAACATTGTTTCTGATACTCCAAAGCAATCTGTGTCGAATAAAAGCATTTTT
TCTGGCCTTTTAAATACACAGTTATGGTCGCAGTTCCGAGGAGATGGGTTCTCTCTGCGCGTCCCCCCAGATTTTGATGATCTCACAGAGCCCGAGGATTACTCAGCAGGTGCATCATTATATGGAGAACGAGCAAAGCCCAAGACTTTTGCTGCACGTTTTGCATCATCTGATGGgtcagaagtattgagcgtggtgat
ccgaccttccactgatctcaagttgacttttttggaggccaaggacatctcagatttgggttctttaagggatgctgccagcctttttgttccagggggagcaacattgtttgctgctcggacaataaaagttaaggacccaataattccgagGACATACTATTTTTATGAATTTTCCGCACAAGACAGGCATGTTGCTT
TAGAAGCTGCTGTTGCTCGTGGGAAGGTATTTGTTGCAGGAGCATCAGCTCCACGATCTAAGTGGGAGGAGGATGGTAGTAAGCTGCGTTCAGCGGCAATATCATTTTCACTTGTATAAATCTCGAGTTTAAGACACTGTAGTATAAGCCGTTCAAGCAAACTTGGAGTATACAAGTTCAAGGATAGAAGCTTCTACATG
AGGCAAAGTTGTGTTGCACTTGAGAAAGAATGCATGACTGTTGACCTGAATCTCTCACGGTCCTTAGAAGGAGAAGACTGATTGTAAACTAGAAATGTAAGTTTGAGAAATTTTTCAAGTTCTTAAACAGTGCATTTGATGCAATATGTTTTTAATGAATAAAGATTTATAGTGAAGCTCCTCTAAAGGGGAAGCTATCT
TGTATTATTAGATGCTTAGAATACTTTTTGTCTACATATCATCCGTTATTGGATTCAGTTCCAGCTCCCACTTCAAATAAAGCTCTATTTGATTTTAAATTGGCAAAAATACTGGTATGTTTGAGTGTCAACTTGTGATGTAGCTTGTCTGTTTCATCTTTTAAGAGATGAAGCTTGAAAGACTATTGTTATGGTTTTCG
AGTTGTCTGGGCTTAGATTTCTTGTTCTTTTAACCTCTCATCATAAACTTGGTGTGTTCCTTCCATCCTGTGTTTTTGGCTTATGATTAATAAACCTACCCTTTGAAGAATCTTGTGAGAATGTTAGGATGAAGATGGAGCTAAACCATTGGAATTTTATATTGGTTGATCTGCTCTATTGTTAGTAATACATGAGCC