sp|Q9SRQ3|PE191_ARATH ----> Peroxisome biogenesis protein 19-1

Intron 1 GTGCCTTGGA ... TTTTGGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50.48	156	164	154
A2	47.66	207	205	172
A3	50.69	178	189	177
A4	49.64	173	177	164
A5	47.42	117	114	97
A6	48.17	440	435	383
A7	49.27	782	806	713
A8	49.22	405	413	372
A9	49.31	715	733	658
A10	49.65	725	759	671

>TRINITY_DN96962_c0_g1_i3 from sample A4 / Example spliced isoform

CCTCTATGATTGCGAATTCTCTTTCCAATACTTGTTGGATAAAACATCTCGAGTATATATCGACTTGATTAACGCAGTTCGACTTTACCTCAAGACCTCTATGATTGCATATTCTCTCTTGCCTGCGGCTTGGACATTGAATACTAAGTTGGATTTGTGCTATTGATGTGAGCTCGACAGTGTCACATTATTGCCTTTGC
AGGGAGGAGGAAGAGGAGGCTGACCAGGAAGCTCGATCTCGATGGCTGATTCTCATAGTTCTCACAATGACTTCGATGATCTTCTTGAGACTTGAATTGGACTCAACTCAGAGGGCTGCAACAACTGGCAATTTATCCAACTCAGTTAAAGAAAGGCAGGAGGAACATTCAGTGCAAGGCTTGGGTATAGGATTGCCGAG
TCCCCCCATCAAGAGGAAGGGGAAGCAGAAGATTGGAACCTCTTCTAAGCCAATGTCTACAGATGCCCATACGGCTGAAACTCTGGAAAAGCTTGCCCAGCAGACCAGACAGACATTGCAGGGTCTGGATTCAGTTGCCCAGGATGATGCTACTGGAGAAAAGCTGGTTGAAAATCTCTTCAAACAATTCGAGGAGATCG
GTGGAACCCAGGATATGCAATCTGTTGTTGACACAATGATGCACCAACTATTGTCTAAGGAGATTCTCCACGAACCAATGAAGGAGATAGGAGAGCGATATCCTAAATGGTTGGAAGAAAGAGCAGCTACAATTAGCAAAGAGGATTATGAGCGCTATTCTCGCCAATATGAACTTATCAAGCAACTTTGCAAAGTATAT
GAGGCAGAGCCAGAAAATTTTGATAAAATAGTTGAACTTATGCAGAAAATGCAAGACTGTGGTCAACCTCCAAGTGATATTGTGCAAGAGCTTGCTCCTGGTCTTAATCTAGGAAGTGAAGGTGTTCCAGCGCTTTCTGAACTTTTTGCAGATAATTCCGGGTCAAATTGCAGTATCATGTAAAGTGGGTACGTTGAAAT
CCCCTTGTGCATGAATCATAGCTCAATTCATTGTGATACTACATTCATTGTCTTACCAAAAGATCGGCTCACAAGAATTCCTGATGGGAGTATACTGAACAACATCACTTTCCATGGAATGCATATCACAATTTGGGACATTTGTCTAAGCTGAAACATATTGTCGTTTTTAGCATCTCATTTGTTTTCCTTCAATCTTC
AGGTGAAAGGTGGGTTGAAATAACTTGTGCTGTCTGCCAATTCTTGTTATGGAAAGAGATATCTTTCCATTTTGATATCTCTGCTTAAAAATATAGGACTGAATTTCATGTAAATTACTTCTTTTATTTAATAAATCCCCGAGTAAAGAAAAATATGTCAATGCGAGTAAACAATTTCTCACATGAAATGACTGTTGACT
GCCTATCTATATTACATAGCAATATGGTTCAATCCCCTC

>TRINITY_DN96962_c0_g1_i4 from sample A4 / Example unspliced isoform:

CCTCTATGATTGCGAATTCTCTTTCCAATACTTGTTGGATAAAACATCTCGAGTATATATCGACTTGATTAACGCAGTTCGACTTTACCTCAAGACCTCTATGATTGCATATTCTCTCTTGCCTGCGGCTTGGACATTGAATACTAAGTTGGATTTGTGCTATTGATGTGAGCTCGACAGTGTCACATTATTGCCTTTGC
AGGGAGGAGGAAGAGGAGGCTGACCAGGAAGCTCGATCTCGATGGCTGATTCTCATAGTTCTCACAATGACTTCGATGATCTTCTTGAGAgtgccttggatgattttggaaagCTTGAATTGGACTCAACTCAGAGGGCTGCAACAACTGGCAATTTATCCAACTCAGTTAAAGAAAGGCAGGAGGAACATTCAGTGCAA
GGCTTGGGTATAGGATTGCCGAGTCCCCCCATCAAGAGGAAGGGGAAGCAGAAGATTGGAACCTCTTCTAAGCCAATGTCTACAGATGCCCATACGGCTGAAACTCTGGAAAAGCTTGCCCAGCAGACCAGACAGACATTGCAGGGTCTGGATTCAGTTGCCCAGGATGATGCTACTGGAGAAAAGCTGGTTGAAAATCT
CTTCAAACAATTCGAGGAGATCGGTGGAACCCAGGATATGCAATCTGTTGTTGACACAATGATGCACCAACTATTGTCTAAGGAGATTCTCCACGAACCAATGAAGGAGATAGGAGAGCGATATCCTAAATGGTTGGAAGAAAGAGCAGCTACAATTAGCAAAGAGGATTATGAGCGCTATTCTCGCCAATATGAACTTA
TCAAGCAACTTTGCAAAGTATATGAGGCAGAGCCAGAAAATTTTGATAAAATAGTTGAACTTATGCAGAAAATGCAAGACTGTGGTCAACCTCCAAGTGATATTGTGCAAGAGCTTGCTCCTGGTCTTAATCTAGGAAGTGAAGGTGTTCCAGCGTATGTTTTCTATATGATGTTTTCTTATAATTTATTTTCAATATGT
AGATGTAACAATAACATTG

Intron 2 GTACGTTGAA ... CAATCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.75	21.8	58.8	29
A2	73.3	14.2	56.5	21
A3	68.12	22.5	61.4	32
A4	71.43	14.2	55.8	11
A5	60.09	13.2	23.9	15
A6	59.17	215.4	334.7	287.2
A7	63.68	184.5	399.8	240
A8	58.25	326.1	450.8	456
A9	59.04	256	402.3	333.2
A10	67.79	106.2	272.1	171

>TRINITY_DN109708_c0_g1_i4 from sample A1 / Example spliced isoform

GGTAAACCCTCTATGATTGCGAATTCTCTTTCCAATACTTGTTGGATAAAACATCTCGAGTATATATCGACTTGATTAACGCAGTTCGACTTTACCTCAAGACCTCTATGATTGCATATTCTCTCTTGCCTGCGGCTTGGACATTGAATACTAAGTTGGATTTGTGCTATTGATGTGAGCTCGACAGTGTCACATTATTG
CCTTTGCAGGGAGGAGGAAGAGGAGGCTGACCAGGAAGCTCGATCTCGATGGCTGATTCTCATAGTTCTCACAATGACTTCGATGATCTTCTTGAGAGTGCCTTGGATGATTTTGGAAAGCTTGAATTGGACTCAACTCAGAGGGCTGCAACAACTGGCAATTTATCCAACTCAGTTAAAGAAAGGCAGGAGGAACATTC
AGTGCAAGGCTTGGGTATAGGATTGCCGAGTCCCCCCATCAAGAGGAAGGGGAAGCAGAAGATTGGAACCTCTTCTAAGCCAATGTCTACAGATGCCCATACGGCTGAAACTCTGGAAAAGCTTGCCCAGCAGACCAGACAGACATTGCAGGGTCTGGATTCAGTTGCCCAGGATGATGCTACTGGAGAAAAGCTGGTTG
AAAATCTCTTCAAACAATTCGAGGAGATCGGTGGAACCCAGGATATGCAATCTGTTGTTGACACAATGATGCACCAACTATTGTCTAAGGAGATTCTCCACGAACCAATGAAGGAGATAGGAGAGCGATATCCTAAATGGTTGGAAGAAAGAGCAGCTACAATTAGCAAAGAGGATTATGAGCGCTATTCTCGCCAATAT
GAACTTATCAAGCAACTTTGCAAAGTATATGAGGCAGAGCCAGAAAATTTTGATAAAATAGTTGAACTTATGCAGAAAATGCAAGACTGTGGTCAACCTCCAAGTGATATTGTGCAAGAGCTTGCTCCTGGTCTTAATCTAGGAAGTGAAGGTGTTCCAGCGCTTTCTGAACTTTTTGCAGATAATTCCGGGTCAAATTG
CAGTATCATGTAAAGTGGGTGAAAGGTGGGTTGAAATAACTTGTGCTGTCTGCCAATTCTTGTTATGGAAAGAGATATCTTTCCATTTTGATATCTCTGCTTAAAAATATAGGACTGAATTTCATGTAAATTACTTCTTTTATTTAATAAATCCCCGAGTAAAGAAAAATATGTCAATGCGAGTAAACAATTTCTCACAT
GAAATGACTGTTGACTGCCTATCTATATTACATAGCAATATGGTTCAATCCCCTATTCTGCATATATTTGTAATCTTTCTTATATTACATTTGCAGATTAGCTAATTCCAAGTGCAATGAAAGTATCTTGGCCTAATTCTGGGCATTTGTTATATAAAGTTAGTAACAGACTTTGTTTTTTCCTTAATTTTTGAAGCCT

>TRINITY_DN109708_c0_g1_i3 from sample A1 / Example unspliced isoform:

GGTAAACCCTCTATGATTGCGAATTCTCTTTCCAATACTTGTTGGATAAAACATCTCGAGTATATATCGACTTGATTAACGCAGTTCGACTTTACCTCAAGACCTCTATGATTGCATATTCTCTCTTGCCTGCGGCTTGGACATTGAATACTAAGTTGGATTTGTGCTATTGATGTGAGCTCGACAGTGTCACATTATTG
CCTTTGCAGGGAGGAGGAAGAGGAGGCTGACCAGGAAGCTCGATCTCGATGGCTGATTCTCATAGTTCTCACAATGACTTCGATGATCTTCTTGAGAGTGCCTTGGATGATTTTGGAAAGCTTGAATTGGACTCAACTCAGAGGGCTGCAACAACTGGCAATTTATCCAACTCAGTTAAAGAAAGGCAGGAGGAACATTC
AGTGCAAGGCTTGGGTATAGGATTGCCGAGTCCCCCCATCAAGAGGAAGGGGAAGCAGAAGATTGGAACCTCTTCTAAGCCAATGTCTACAGATGCCCATACGGCTGAAACTCTGGAAAAGCTTGCCCAGCAGACCAGACAGACATTGCAGGGTCTGGATTCAGTTGCCCAGGATGATGCTACTGGAGAAAAGCTGGTTG
AAAATCTCTTCAAACAATTCGAGGAGATCGGTGGAACCCAGGATATGCAATCTGTTGTTGACACAATGATGCACCAACTATTGTCTAAGGAGATTCTCCACGAACCAATGAAGGAGATAGGAGAGCGATATCCTAAATGGTTGGAAGAAAGAGCAGCTACAATTAGCAAAGAGGATTATGAGCGCTATTCTCGCCAATAT
GAACTTATCAAGCAACTTTGCAAAGTATATGAGGCAGAGCCAGAAAATTTTGATAAAATAGTTGAACTTATGCAGAAAATGCAAGACTGTGGTCAACCTCCAAGTGATATTGTGCAAGAGCTTGCTCCTGGTCTTAATCTAGGAAGTGAAGGTGTTCCAGCGCTTTCTGAACTTTTTGCAGATAATTCCGGGTCAAATTG
CAGTATCATGTAAAGTGGgtacgttgaaatccccttgtgcatgaatcatagctcaattcattgtgatactacattcattgtcttaccaaaagatcggctcacaagaattcctgatgggagtatactgaacaacatcactttccatggaatgcatatcacaatttgggacatttgtctaagctgaaacatattgtcgtttt
tagcatctcatttgttttccttcaatcttcagGTGAAAGGTGGGTTGAAATAACTTGTGCTGTCTGCCAATTCTTGTTATGGAAAGAGATATCTTTCCATTTTGATATCTCTGCTTAAAAATATAGGACTGAATTTCATGTAAATTACTTCTTTTATTTAATAAATCCCCGAGTAAAGAAAAATATGTCAATGCGAGTAA
ACAATTTCTCACATGAAATGACTGTTGACTGCCTATCTATATTACATAGCAATATGGTTCAATCCCCTATTCTGCATATATTTGTAATCTTTCTTATATTACATTTGCAGATTAGCTAATTCCAAGTGCAATGAAAGTATCTTGGCCTAATTCTGGGCATTTGTTATATAAAGTTAGTAACAGACTTTGTTTTTTCCTTA
ATTTTTGAAGCCT