sp|F4JC97|PSME4_ARATH ----> Proteasome activator subunit 4


Intron 1 GTCATTATTA ... TTTGATTTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A112.52871
A225301010
A339.62161011
A415.663549
A529.411282
A620420
A725320
A825320
A920603
A1029.41641



>TRINITY_DN121464_c1_g1_i4 from sample A3 / Example spliced isoform
AACCAAGCGAACCAGCATAAGGGGTTTTGGGCCGATACTTTGTCCACGTGACCAACCAGCAAAAACTTGTTGGGGAAGCCTGCCCAATTGAAGCCACAGTCCAAACAAACTAAACGTACGCCTCAGTATCAAAATTTAGAACACGCAAGACTTTCATCGGAGACATTCATTGGAGGCGACCATAAATGACAATTTCTTGG
CCTTGAAGCAATCGTTCATCGTTAAATTTGTTCCGATTCTTCGTTGCAGAGCTCAGATCCCTCCCGTTGACTCTTTGAATTAAAGCGCACTGCAAAAAGGCCGGACATTTATTTGCAATCGAAGATGCATCTTTACAATGCTTGGTTGCCTCCTCCGGTGGCGGAGCTTGCCGTTAAAGAAAAGGACGCTTTTACGGAAG
TAGTGAACACAGTAAAAGATGCATGGAAGCCTGACGATCCTGGCTCTGCATATGCTACTCTCAAGTGGATTCCGATCTTGCACAATTTTGTAAAAGCAAAAAGTGAAATTGCTTTAGAAGATGTGAAAGTCTTAGTTTTGATTGGGTTGGATCTTCTTCTTTGTTCACAAGATCACCTTGATGTCCAGGTGAGATGGGGC
AATCTCATTTCGAGGTTATTGAGAAAATATGGAAAGAAACTAGATATTACAATACAGTGGCGACCATTATATGATCTTTTGGTACAAACACACTTCACAAGGCATCGGAGTGCTGATGGTTTAAGGTTGAAGCATAGTCACTTGGAGACAATGATATACCTCATTTATTCTTGTCGGCAGTTTTTCTCATCAGGCTCTGC
AGCAGAAATATGGATAGAATTCAGGCCTTTTATGGAGAATTTATGGCATAACTCGTCACTGGAAGCTGCTGGTTTTGTTCAGTTGTTTCTTCCAACAAATGCAGTAAACCTGGACTTTTTTAATTGTGACTGGATGACAGATTGCTTGAACCTATGGGTTGGAATGCCAAATTGTCAATACTGGGATTTTCAATGGGCTG
CTGTCATGGCACGTTGTATAAAACACTCTATGACAATCGAATGGGAGGGTTTTCTTCCAGTGCTTTTCACACAATACTTGAATATGTTCGAGGTACCGGTGGCAAATAACAGTGGGCCATACCCTTTTTCAAGAGATGATGTTCCGAGAAGTACCATATACGCATTTTCTCAAAGCAAGCTAGTTACACCTTCAAAAGAG
ATTGCTAAATCAATTGTATATCTGCTCAAACCTGGTGGTTCGGCTCAAGCTTACCTTGAAAAATTGGTTGACCTTCTAGAACAATATTATCATCCATCTAATGGGGGTCGGTGGAGTTACTCTTTAGAACGCTTTTTGCGATATCTGGTGTACTACTTTCAGAAACGCCTTGCCCAAGAGCAACGAAGTAGAGAGCTTGG
AACAGGCATGGTTCAAAACTTCCTTGGAAGCTCAGAAAGGGAAGCCTTTGTAAGATCTGTTTTGAAATTGATTGAGCGTGGTCAGTACAGCAAGAGTGAATCCCTTGCTGGAACTGCTGCTGTTGCATCTTCTGTGTTATCCTATGTGGAGCCCACTGTTGTTCTGCCCTTTATTGTGTCAAGCTTCCATATTGCACTTG
ACAATGTAATAGCCCCACATCAATTTAAGTCTGCAATCACAGGAATGGCGCTTGCCAGTCGTGCTTTACTTCTAGCTTCTGCAGAAAGTTCTTATTTGGAAACTGACAAATCTGAATGTAATATTTCTCCTACTGGTTACAACCATGCCTTAATGGTTTCCATGTGCAATACTTTACCTGGTATGGATGTCAATGATCCT
CCGAAAACCCTTGCAACTATGCAGCTCTTTGGATCAATATTCTCTAATCTAGCTGAGGTTGGTGATAAAGAAGATGGCAGCACACCTATTCTGGGTATCGATTTATCTGAATGGCTGGATGAATTCTTTTGTCGTCTTTTTTCTCTACTTATACATTTGGAACCAAACAATCAATCAAATGAAGGACAATTTTTATCAAC
ATCTTCAGGAACTTTCCTTGTTGAAGATGGACCTTACTATTTTTGTATGCTGGAAATTTTACTTGGTAAACTCACAAGACCTCTTTACCATCAGGCATTGAAAAGATTTCCAGGTTTGTTCATGGTCATACTCTCCCGGGAGCAGTTGATGAAATTGGATTACTGTGTTCTGCTACATTATATTCTAATCCAGCAGAGGC
ATTAGTGCACCTGCTTCAGCCCATGATAAACTCTGTTATATCTTCTCTTAAAGAATCTCCATCGACTGGATGTAGTAGTCGTGAAATTTCATACGCATCATCTGTAACAGAGGCATCATTATCTCCAGCTTTGGAAGCATCGGTAGCATACCAATTGAATGTTCTTTCTGTTGCCATCAACTTTGGGGGTCCACATATTC
TTCAGTGCAAAGAAGAAATAAAAGAAGCTATTTCAGCTGCTTTTGATGCACCATCTTGGAAGGTGAATGAGGCTGGAAACCATCTTTTGAGGTCCCTTATTGGAAGCCTATCTCTTTACTACCCACTGGACCAGTATAAGTGCTATTCTCGTTATCCTGGCATTGATGGGCTAGAGAAATGGATTAGCACGAAGGAAAAT
CATAATGAAGAAGTTTCAGTGGGCCCTAAATGGCATATTCCAAATAAAGATGAGATAGCATTTGCGAATGAGCTCCTGGACCTGAATCTGAGGTCAGCTTTGGACAAGCTTAAGCAAATCTGTCAATCACAGCATCCCTTAGAATCAGGATATGAAAAGCAGCTCCTGAGAGTCATTCTTTTGCAAATTGATGCATCACT
TCAAGGCGCTCGGTCTTGTTTACCTGATTTTCACCCACAACAAGGAGATGCAAAACATGATGATGAGAAATACAGCACCTCATATATTTCGGGAGCATCTGGTGTATGTATTGGCAGTACTGAACTAAGGCAAGAGGCTGCAGATGTAGTAGATTTTGCCTGCAAATATATGTTGGAGCATAGAGCAGACAATAGCATAT
TGCTGATGCTTCTTGTTCGTATTTTGGATGCATTGGTGAACTATGGGAGCTTGGAATATGATGAGTGGTCAGACCACAAACATGCATGGACAATGGACTCATCCGCAATAAAAGAGCCTCCATCAAACTTTATAACTGGTCATCATGCTCAAGGAAAGCGGAGACCTAGGTGGACACTCATAGATAGATCATACATGCAT
AATACATGGCGAGCATCACAATCAAGTTACCATCAGTTTCGCATTGATAGCAGCATCCGCCCACCAGAGCATATCATTCTTTTGATGAATGATCTTTTGGAACTTTCCTTTAATGACTACGAGGCAGTTCGTCTCCGTGCAGGGAAGTCACTGGTGAAGATGCTGAAGCGATTCCCATCTCTAGTTAAAGATTGTATGCC
AAAACTCACGACTAGCTTAGGAACTCCAGGAATTTCTGAAAATGCTGCCTTGGGGGCTTGTACAGTACTTAAATCCAGAACTGTAATGAGGCACATAATGAAGGATTGGGATGCTTTTTCATCTTTCGTCTTGGCTATACTTGGAAGTTCCCATCAAGGATCATTGAGAGCTCAAAAATCCATTAATGAGCTCTTTCTTG
TGTTTAACATACGTTATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATTCCGATTGCATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTGGCACTTCCTTCAAATAATAATTTTGAT
TTGCCATCAAAAACAAGAGAGGAAATTGCTTGTCATTTCTTGTTACTTCTCAATAGCCATCTTCCTCAAGTAAGGGTTCTTGCAGCTGCCTCCCTTGTTCTTCTTCTGCAAGCATCCCCCAACAAGAAATCAGTTAGTGATAACTTGTTGCATTCTGGATGGTCAAATGTAATTGCTGGTTCTTCTTTCAAGGGTGTTTT
GAATCCAATCATTCAGGAAGAAGGCTTTGTCAATGACATGCTGAATGGTTTATCTTATGACCATGTTTTTCCTGATGCAAACAACTCAAACAACCATGCTATGGCTATGTCCCAAAGTATTCGAGATAAATCAGTGAATGGATGGTATGAGGCATTTTATGCTCCATGGCCACGCTCAAGAAACTGGATTGACCTCTTAA
AAGGAGAGGCATTTGTACCTGGTTTTGCCAAGTTATTTAAATGCCTTGTTAAAGAGTGTGGAGAAACAGTACTTCAATCTTTGCGGGGCCCTTTAGAAGAGTTGGTTGCAAAGGAAGAAAAGGGCAAACAGTGCCTTGCTTCCGAGGTTTTGGCCGGTGTCTTGCATTCTGATGTTAACTATATTTTAGAGGCCTGGGAC
ATTTGGCTATGTGCTGTGCTGCGAACAGGACTACGCCAGTCAACTGTTGAAACAGCTGCTGAGTGGGCTGCCTGTGTGCGATTCGCAGTTACTGGAAAAGGAAGGGATGGGAAAACTATACCATTGTTGCGGCAAAAAATTATGGAATGCCTTGCTGAACCATTACCAAGTACTGTTCCAACTCGCATGGTGGCTAAAAG
GTTTGATCTTCTATGTGCAACGTTAGTAGAGGTGTCCCCTTCCTCCATGTCAGCTTCAGAAATCAACTTACAAGAGAAATTATTAGAGGAGGTTTTAAATTGCTTGGATCATTCTGTTGCTCAGGTACGGGAATCTGTTGGGACCACTTTTAGTGTTTTGAGCTCAAACATTCGGCTTTCCTCATCTTTTGGTGATCGGG
CATCCAATCCAAACAGAAGACTTGATTGGAGTGTGCTTCTTATTGAAAAAGCTGCTATTTTCACGACAAAGATTCACATTGCCAACCAATCTACTGTGGTTGATGAGCAGTTGGTTTCTTCTGATGATAACAATGGTGAAGGTGACACTGAGCAGCAAGAGCATATAAAATGGCTGGAAACAGTGTTCTATTTTCTTATT
TCAGCTTTAAGGTCTGGGAGAGCGTCAGTTTTGACAGATGTCATTGTTGGTCTTCTGTATCCTCTTATTTCTCTGCAGGAGACATCACATAAGGAATTATCTACTTTAGCTAAAAAGGCTTTACAACTGTTAAAGTGGCATATAATTCCACAGCCACATCTGTCAACATCCGTCACTGTACTTATATCTTCAAGTAAGGA
CTCTAGTTGGCATACCAGAGTTGCAACATTAATATTCTTGCAATCCTTCATGTACAGGCATAAGTTCCTCCTGTCAAGCTCCGAGACAGAACTTGTGTGGAATCAATTGCAAAAGCTTCTTGCAGACAATCAAGTTGAGGTTAGAGAGCAAGCAGCCTCTGTGCTATCGGGTTTAATGAGAGGAAGCGACGATAAATTGG
TCAAGCCTTTTCGTGAGAATTCATTCAAAGAAGCGATTTTGCTACAAAAGAGAAGCAAGACAAGGTTGTCAGAAAATGGCCTATCTTTAGCTTCTAGGCATGGCGTGGTGTTAGCTTTGGCATCTTCAATATTATCAGTGCCATATGACCTGCCAAGCTGGCTGCCTGATACTATAACTTTATTGGCACGCTTCATTGGA
GAACCATCACCAATAAGATCAACTGTAATGAAAACAATTGCCGAGTTCCGTAGAACACATGCAGACACTTGGGCCATCCAAAAGGATTCCTTCACTGAAGACCAACTAGAGGTTTTAGCTGACTCATCATCATCGTTGTCATATTTTTCATGATTGAAAAATAGGCTGGGCTCGACTTGTCCTTTGCAATTAAGGAGGTC
TTCCAAAACTTTGTCAGCAATCAATAAAATGGATAATTAATATCACACCAGCATTATTGCAGCTGAAAACAGAGCTCTGGCCTTTACAAATATTTATCTATTGGTGCATTAACACTTTCATTTTTAGCAGACATCAAAGATTTTGTCTATCAAAATTGCAATTAAGCCCGTGAGAACATTTTTGGGTTCTGTATTTTTTG
TGTGATCCTGATTGGTTTGAAAGTGATCACATGTAATAATAATGATGACATCCCCATAGCTGTTAAAAAGCTTGTTCTTGGGGGTGGGTCCACTGGAACTTAAGTCTTATTTCAAATGCAGACTATTATTAATGAGGAG


>TRINITY_DN121464_c1_g1_i11 from sample A3 / Example unspliced isoform:
AACCAAGCGAACCAGCATAAGGGGTTTTGGGCCGATACTTTGTCCACGTGACCAACCAGCAAAAACTTGTTGGGGAAGCCTGCCCAATTGAAGCCACAGTCCAAACAAACTAAACGTACGCCTCAGTATCAAAATTTAGAACACGCAAGACTTTCATCGGAGACATTCATTGGAGGCGACCATAAATGACAATTTCTTGG
CCTTGAAGCAATCGTTCATCGTTAAATTTGTTCCGATTCTTCGTTGCAGAGCTCAGATCCCTCCCGTTGACTCTTTGAATTAAAGCGCACTGCAAAAAGGCCGGACATTTATTTGCAATCGAAGATGCATCTTTACAATGCTTGGTTGCCTCCTCCGGTGGCGGAGCTTGCCGTTAAAGAAAAGGACGCTTTTACGGAAG
TAGTGAACACAGTAAAAGATGCATGGAAGCCTGACGATCCTGGCTCTGCATATGCTACTCTCAAGTGGATTCCGATCTTGCACAATTTTGTAAAAGCAAAAAGTGAAATTGCTTTAGAAGATGTGAAAGTCTTAGTTTTGATTGGGTTGGATCTTCTTCTTTGTTCACAAGATCACCTTGATGTCCAGGTGAGATGGGGC
AATCTCATTTCGAGGTTATTGAGAAAATATGGAAAGAAACTAGATATTACAATACAGTGGCGACCATTATATGATCTTTTGGTACAAACACACTTCACAAGGCATCGGAGTGCTGATGGTTTAAGGTTGAAGCATAGTCACTTGGAGACAATGATATACCTCATTTATTCTTGTCGGCAGTTTTTCTCATCAGGCTCTGC
AGCAGAAATATGGATAGAATTCAGGCCTTTTATGGAGAATTTATGGCATAACTCGTCACTGGAAGCTGCTGGTTTTGTTCAGTTGTTTCTTCCAACAAATGCAGTAAACCTGGACTTTTTTAATTGTGACTGGATGACAGATTGCTTGAACCTATGGGTTGGAATGCCAAATTGTCAATACTGGGATTTTCAATGGGCTG
CTGTCATGGCACGTTGTATAAAACACTCTATGACAATCGAATGGGAGGGTTTTCTTCCAGTGCTTTTCACACAATACTTGAATATGTTCGAGGTACCGGTGGCAAATAACAGTGGGCCATACCCTTTTTCAAGAGATGATGTTCCGAGAAGTACCATATACGCATTTTCTCAAAGCAAGCTAGTTACACCTTCAAAAGAG
ATTGCTAAATCAATTGTATATCTGCTCAAACCTGGTGGTTCGGCTCAAGCTTACCTTGAAAAATTGGTTGACCTTCTAGAACAATATTATCATCCATCTAATGGGGGTCGGTGGAGTTACTCTTTAGAACGCTTTTTGCGATATCTGGTGTACTACTTTCAGAAACGCCTTGCCCAAGAGCAACGAAGTAGAGAGCTTGG
AACAGGCATGGTTCAAAACTTCCTTGGAAGCTCAGAAAGGGAAGCCTTTGTAAGATCTGTTTTGAAATTGATTGAGCGTGGTCAGTACAGCAAGAGTGAATCCCTTGCTGGAACTGCTGCTGTTGCATCTTCTGTGTTATCCTATGTGGAGCCCACTGTTGTTCTGCCCTTTATTGTGTCAAGCTTCCATATTGCACTTG
ACAATGTAATAGCCCCACATCAATTTAAGTCTGCAATCACAGGAATGGCGCTTGCCAGTCGTGCTTTACTTCTAGCTTCTGCAGAAAGTTCTTATTTGGAAACTGACAAATCTGAATGTAATATTTCTCCTACTGGTTACAACCATGCCTTAATGGTTTCCATGTGCAATACTTTACCTGGTATGGATGTCAATGATCCT
CCGAAAACCCTTGCAACTATGCAGCTCTTTGGATCAATATTCTCTAATCTAGCTGAGGTTGGTGATAAAGAAGATGGCAGCACACCTATTCTGGGTATCGATTTATCTGAATGGCTGGATGAATTCTTTTGTCGTCTTTTTTCTCTACTTATACATTTGGAACCAAACAATCAATCAAATGAAGGACAATTTTTATCAAC
ATCTTCAGGAACTTTCCTTGTTGAAGATGGACCTTACTATTTTTGTATGCTGGAAATTTTACTTGGTAAACTCACAAGACCTCTTTACCATCAGGCATTGAAAAAGATTTCCAGGTTTGTTCATGGTCATACTCTCCCGGGAGCAGTTGATGAAATTGGATTACTGTGTTCTGCTACATTATATTCTAATCCAGCAGAGG
CATTAGTGCACCTGCTTCAGCCCATGATAAACTCTGTTATATCTTCTCTTAAAGAATCTCCATCGACTGGATGTAGTAGTCGTGAAATTTCATACGCATCATCTGTAACAGAGgtcattattagttgtagtaaagattgaattatgttttatgatatccattcttgatctatagaacattcagtgaaattttcctttcat
atcctgtattgtttgatttagGCATCATTATCTCCAGCTTTGGAAGCATCGGTAGCATACCAATTGAATGTTCTTTCTGTTGCCATCAACTTTGGGGGTCCACATATTCTTCAGTGCAAAGAAGAAATAAAAGAAGCTATTTCAGCTGCTTTTGATGCACCATCTTGGAAGGTGAATGAGGCTGGAAACCATCTTTTGAG
GTCCCTTATTGGAAGCCTATCTCTTTACTACCCACTGGACCAGTATAAGTGCTATTCTCGTTATCCTGGCATTGATGGGCTAGAGAAATGGATTAGCACGAAGGAAAATCATAATGAAGAAGTTTCAGTGGGCCCTAAATGGCATATTCCAAATAAAGATGAGATAGCATTTGCGAATGAGCTCCTGGACCTGAATCTGA
GGTCAGCTTTGGACAAGCTTAAGCAAATCTGTCAATCACAGCATCCCTTAGAATCAGGATATGAAAAGCAGCTCCTGAGAGTCATTCTTTTGCAAATTGATGCATCACTTCAAGGCGCTCGGTCTTGTTTACCTGATTTTCACCCACAACAAGGAGATGCAAAACATGATGATGAGAAATACAGCACCTCATATATTTCG
GGAGCATCTGGTGTATGTATTGGCAGTACTGAACTAAGGCAAGAGGCTGCAGATGTAGTAGATTTTGCCTGCAAATATATGTTGGAGCATAGAGCAGACAATAGCATATTGCTGATGCTTCTTGTTCGTATTTTGGATGCATTGGTGAACTATGGGAGCTTGGAATATGATGAGTGGTCAGACCACAAACATGCATGGAC
AATGGACTCATCCGCAATAAAAGAGCCTCCATCAAACTTTATAACTGGTCATCATGCTCAAGGAAAGCGGAGACCTAGGTGGACACTCATAGATAGATCATACATGCATAATACATGGCGAGCATCACAATCAAGTTACCATCAGTTTCGCATTGATAGCAGCATCCGCCCACCAGAGCATATCATTCTTTTGATGAATG
ATCTTTTGGAACTTTCCTTTAATGACTACGAGGCAGTTCGTCTCCGTGCAGGGAAGTCACTGGTGAAGATGCTGAAGCGATTCCCATCTCTAGTTAAAGATTGTATGCCAAAACTCACGACTAGCTTAGGAACTCCAGGAATTTCTGAAAATGCTGCCTTGGGGGCTTGTACAGTACTTAAATCCAGAACTGTAATGAGG
CACATAATGAAGGATTGGGATGCTTTTTCATCTTTCGTCTTGGCTATACTTGGAAGTTCCCATCAAGGATCATTGAGAGCTCAAAAATCCATTAATGAGCTCTTTCTTGTGTTTAACATACGTTATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATTCCGATTGCATGAATTTGATCAC
TCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTGGCACTTCCTTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGGAAATTGCTTGTCATTTCTTGTTACTTCTCAATAGCCATCTTCCTCAAGTAAGGGTTCTTGCAGCTGCCT
CCCTTGTTCTTCTTCTGCAAGCATCCCCCAACAAGAAATCAGTTAGTGATAACTTGTTGCATTCTGGATGGTCAAATGTAATTGCTGGTTCTTCTTTCAAGGGTGTTTTGAATCCAATCATTCAGGAAGAAGGCTTTGTCAATGACATGCTGAATGGTTTATCTTATGACCATGTTTTTCCTGATGCAAACAACTCAAAC
AACCATGCTATGGCTATGTCCCAAAGTATTCGAGATAAATCAGTGAATGGATGGTATGAGGCATTTTATGCTCCATGGCCACGCTCAAGAAACTGGATTGACCTCTTAAAAGGAGAGGCATTTGTACCTGGTTTTGCCAAGTTATTTAAATGCCTTGTTAAAGAGTGTGGAGAAACAGTACTTCAATCTTTGCGGGGCCC
TTTAGAAGAGTTGGTTGCAAAGGAAGAAAAGGGCAAACAGTGCCTTGCTTCCGAGGTTTTGGCCGGTGTCTTGCATTCTGATGTTAACTATATTTTAGAGGCCTGGGACATTTGGCTATGTGCTGTGCTGCGAACAGGACTACGCCAGTCAACTGTTGAAACAGCTGCTGAGTGGGCTGCCTGTGTGCGATTCGCAGTTA
CTGGAAAAGGAAGGGATGGGAAAACTATACCATTGTTGCGGCAAAAAATTATGGAATGCCTTGCTGAACCATTACCAAGTACTGTTCCAACTCGCATGGTGGCTAAAAGGTTTGATCTTCTATGTGCAACGTTAGTAGAGGTGTCCCCTTCCTCCATGTCAGCTTCAGAAATCAACTTACAAGAGAAATTATTAGAGGAG
GTTTTAAATTGCTTGGATCATTCTGTTGCTCAGGTACGGGAATCTGTTGGGACCACTTTTAGTGTTTTGAGCTCAAACATTCGGCTTTCCTCATCTTTTGGTGATCGGGCATCCAATCCAAACAGAAGACTTGATTGGAGTGTGCTTCTTATTGAAAAAGCTGCTATTTTCACGACAAAGATTCACATTGCCAACCAATC
TACTGTGGTTGATGAGCAGTTGGTTTCTTCTGATGATAACAATGGTGAAGGTGACACTGAGCAGCAAGAGCATATAAAATGGCTGGAAACAGTGTTCTATTTTCTTATTTCAGCTTTAAGGTCTGGGAGAGCGTCAGTTTTGACAGATGTCATTGTTGGTCTTCTGTATCCTCTTATTTCTCTGCAGGTCATTTCTTGTT
ACTTCTCAATAGCCATCTTCCTCCAGTAAGGGTTCTTGCAGCTGCATCCCTTGTTCTTCTTCTGCAAGC



Intron 2 GTAAGTCTAT ... CTTTTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A128.8769281
A239.9828.5191
A327.0835132
A416.1236.572
A513.332640
A623.3153162
A734.1492.6485
A826.23120.342.715
A921.5598.5274
A1033.7353.1279



>TRINITY_DN48367_c0_g1_i11 from sample A7 / Example spliced isoform
CAGTATCAAAATTTAGAACACGCAAGACTTTCATCGGAGACATTCATTGGAGGCGACCATAAATGACAATTTCTTGGCCTTGAAGCAATCGTTCATCGTTAAATTTGTTCCGATTCTTCGTTGCAGAGCTCAGATCCCTCCCGTTGACTCTTTGAATTAAAGCGCACTGCAAAAAGGCCGGACATTTATTTGCAATCGAA
GATGCATCTTTACAATGCTTGGTTGCCTCCTCCGGTGGCGGAGCTTGCCGTTAAAGAAAAGGACGCTTTTACGGAAGTAGTGAACACAGTAAAAGATGCATGGAAGCCTGACGATCCTGGCTCTGCATATGCTACTCTCAAGTGGATTCCGATCTTGCACAATTTTGTAAAAGCAAAAAGTGAAATTGATTTAGAAGATG
TGAAAGTCTTAGTTTTGATTGGGTTGGATCTTCTTCTTTGTTCACAAGATCACCTTGATGTCCAGGTGAGATGGGGCAATCTCATTTCGAGGTTATTGAGAAAATATGGAAAGAAACTAGATATTACAATACAGTGGCGACCATTATATGATCTTTTGGTACAAACACACTTCACAAGGCATCGGAGTGCTGATGGTTTA
AGGTTGAAGCATAGTCACTTGGAGACAATGATATACCTCATTTATTCTTGTCGGCAGTTTTTCTCATCAGGCTCTGCAGCAGAAATATGGATAGAATTCAGGCCTTTTATGGAGAATTTATGGCATAACTCGTCACTGGAAGCTGCTGGTTTTGTTCAGTTGTTTCTTCCAACAAATGCAGTAAACCTGGACTTTTTTAA
TTGTGACTGGATGACAGATTGCTTGAACCTATGGGTTGGAATGCCAAATTGTCAATACTGGGATTTTCAATGGGCTGCTGTCATGGCACGTTGTATAAAACACTCTATGACAATCGAATGGGAGGGTTTTCTTCCAGTGCTTTTCACACAATACTTGAATATGTTCGAGGTACCGGTGGCAAATAACAGTGGGCCATACC
CTTTTTCAAGAGATGATGTTCCGAGAAGTACCATATACGCATTTTCTCAAAGCAAGCTAGTTACACCTTCAAAAGAGATTGCTAAATCAATTGTATATCTGCTCAAACCTGGTGGTTCGGCTCAAGCTTACCTTGAAAAATTGGTTGACCTTCTAGAACAATATTATCATCCATCTAATGGGGGTCGGTGGAGTTACTCT
TTAGAACGCTTTTTGCGATATCTGGTGTACTACTTTCAGAAACGCCTTGCCCAAGAGCAACGAAGTAGAGAGCTTGGAACAGGCATGGTTCAAAACTTCCTTGGAAGCTCAGAAAGGGAAGCCTTTGTAAGATCTGTTTTGAAATTGATTGAGCGTGGTCAGTACAGCAAGAGTGAATCCCTTGCTGGAACTGCTGCTGT
TGCATCTTCTGTGTTATCCTATGTGGAGCCCACTGTTGTTCTGCCCTTTATTGTGTCAAGCTTCCATATTGCACTTGACAATGTAATAGCCCCACATCAATTTAAGTCTGCAATCACAGGAATGGCGCTTGCCAGTCGTGCTTTACTTCTAGCTTCTGCAGAAAGTTCTTATTTGGAAACTGACAAATCTGAATGTAATA
TTTCTCCTACTGGTTACAACCATGCCTTAATGGTTTCCATGTGCAATACTTTACCTGGTATGGATGTCAATGATCCTCCGAAAACCCTTGCAACTATGCAGCTCTTTGGATCAATATTCTCTAATCTAGCTGAGGTTGGTGATAAAGAAGATGGCAGCACACCTATTCTGGGTATCGATTTATCTGAATGGCTGGATGAA
TTCTTTTGTCGTCTTTTTTCTCTACTTATACATTTGGAACCAAACAATCAATCAAATGAAGGACAATTTTTATCAACATCTTCAGGAACTTTCCTTGTTGAAGATGGACCTTACTATTTTTGTATGCTGGAAATTTTACTTGGTAAACTCACAAGACCTCTTTACCATCAGGCATTGAAAAAGATTTCCAGGTTTGTTCA
TGGTCATACTCTCCCGGGAGCAGTTGATGAAATTGGATTACTGTGTTCTGCTACATTATATTCTAATCCAGCAGAGGCATTAGTGCACCTGCTTCAGCCCATGATAAACTCTGTTATATCTTCTCTTAAAGAATCTCCATCGACTGGATGTAGTAGTCGTGAAATTTCATACGCATCATCTGTAACAGAGGCATCATTAT
CTCCAGCTTTGGAAGCATCGGTAGCATACCAATTGAATGTTCTTTCTGTTGCCATCAACTTTGGGGGTCCACATATTCTTCAGTGCAAAGAAGAAATAAAAGAAGCTATTTCAGCTGCTTTTGATGCACCATCTTGGAAGGTGAATGAGGCTGGAAACCATCTTTTGAGGTCCCTTATTGGAAGCCTATCTCTTTACTAC
CCACTGGACCAGTATAAGTGCTATTCTCGTTATCCTGGCATTGATGGGCTAGAGAAATGGATTAGCACGAAGGAAAATCATAATGAAGAAGTTTCAGTGGGCCCTAAATGGCATATTCCAAATAAAGATGAGATAGCATTTGCGAATGAGCTCCTGGACCTGAATCTGAGGTCAGCTTTGGACAAGCTTAAGCAAATCTG
TCAATCACAGCATCCCTTAGAATCAGGATATGAAAAGCAGCTCCTGAGAGTCATTCTTTTGCAAATTGATGCATCACTTCAAGGCGCTCGGTCTTGTTTACCTGATTTTCACCCACAACAAGGAGATGCAAAACATGATGATGAGAAATACAGCACCTCATATATTTCGGGAGCATCTGGTGTATGTATTGGCAGTACTG
AACTAAGGCAAGAGGCTGCAGATGTAGTAGATTTTGCCTGCAAATATATGTTGGAGCATAGAGCAGACAATAGCATATTGCTGATGCTTCTTGTTCGTATTTTGGATGCATTGGTGAACTATGGGAGCTTGGAATATGATGAGTGGTCAGACCACAAACATGCATGGACAATGGACTCATCCGCAATAAAAGAGCCTCCA
TCAAACTTTATAACTGGTCATCATGCTCAAGGAAAGCGGAGACCTAGGTGGGCACTCATAGATAGATCATACATGCATAATACATGGCGAGCATCACAATCAAGTTACCATCAGTTTCGCATTGATAGCAGCATCCGCCCACCAGAGCATATCATTCTTTTGATGAATGATCTTTTGGAACTTTCCTTTAATGACTACGA
GGCAGTTCGTCTCCGTGCAGGGAAGTCACTGGTGAAGATGCTGAAGCGATTCCCATCTCTAGTTAAAGATTGTATGCCAAAACTCACGACTAGCTTAGGAACTCCAGGAATTTCTGAAAATGCTGCCTTGGGGGCTTGTACAGTACTTAAATCCAGAACTGTAATGAGGCACATAATGAAGGATTGGGATGCTTTTTCAT
CTTTCGTCTTGGCTATACTTGGAAGTTCCCATCAAGGATCATTGAGAGCTCAAAAATCCATTAATGAGCTCTTTCTTGTGTTTAACATACGTTATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATTCCGATTACATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCAT
AGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCACTTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGGAAATTGCTTTTTTCTCTGATAATTGTTTCAGGTGTTATCTGCATTCATTCATTCATCCTGGACTGAATGTGGCTTCTATTTTGCATAATTTTCCACTCTATTTC
CATCAAAAGAAGAGGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCCTAGGAAACAATGGAAGAAAATTGAAATCTCCTTCGCATTCTTGTCTGTCTGTGGATATTCATGCTATTGAAAAAGAGAAAGGTCGAATGGTTCCAAATTGTTATTATCTTTCTGAACTTGTCA
ATAAAGAATTTTTCTCTAC


>TRINITY_DN48367_c0_g1_i10 from sample A7 / Example unspliced isoform:
GTGGAGAGAACCGAAATAGTTGGGGGTCGATTTGGTCAGGATTTGGCGTTAAGGAAAAACCTGCATCAGAGAAGGAAGATGCTCTTGCTGCCACACCCGACGATGATAAACCCACAATCCCTAAAGCCCTAGGACTCTCTGTCTCAATCTGGATTCTTCAGGGTTTAGAACTTTCAGCCGACTCTACGAAAGAAGTATAA
AAAGGGCAAATGAGGGGTATTCTCCTCATTCAACACTTTCATTTTGGGGAAGAAAGCTTGGAGAGCTCTTTGAGTATTTGAGCTAGGGTTTGAGAAAACCCTTGCTTGTTGCGACTGGTTCGGGGGTGAAATATTCCTCCCTAGCTGGTGTGGGTGGATTCATCCAGGATTCACACTTGTACTTCATTGCCATCGTATAT
TATCTTCGATTCCAGTCATATTTGAAGCATTTTGAGGCTAGGGCGCATTTGTGGAGAAATATTCTCAGTCTGGTTTGGATGGCGCACTTGGAGAGGAAGGAACACCATTGCTTGTTAAAGGTGCATTACATCTGCTGATAGGGGTGCACTCGGAGTCTGCTTTGTTCAAGTTTGAGAAGGATGCACTTGAAGATTGTCTT
CATGCTTGGTGGAGGGCGCATTCTAAAAAAGGATCATTCTTATTAACTGTGATATCTAAGTATAAGAAGGGCCCATCTCTTGACTATTAACCAAATCGTGAAGGAGTCTTCTACTACACAAAATCGTAGTCAATAGTGTCCAAACCATTTGCAAAGAAGAGAACAAGTTTCCCCATTGTTCTGCATCTGCAATCACAGTG
GAATAACATCTGGTCGAAACACGAACACTATGGTTGTTTGAAGGGCGAGTTTTGTAGTAACTCTTGCAATCTTGAAGATCGAAATCCAATAGTTTCATGCCAAACAAAGAACAAATTTTGTACAACATTCTGCCATTGAATCTATTAGCCAGGAATGTGTGGGCCTCCTGTACCATCCAGAAATCTGTAGCAGCATCTCC
TTGTTGTTGGGTCAACACACCTCAAAGGCATATATGAATGCCTACAGCTCTTTCTTGTGTTTTAACATATGTTATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATTCCGATTACATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGgtaagt
ctatttccactgtgtttcagaaattacagtttccttgtttctaacagtgcaatattttgtgtttttaatataaatgcaaagattttggatgagtcttacatcttttggctttttgcagATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCACTTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGG
AAATTGCTTGTAAGTTTCTTTGCCCTGTAAGTATTGTAGAGTGAAAAGTAATACCCCAAAACATAATCTTTTTTATCGGCGATGCTGACCATGCTATTTTTAGTTTTCTCTGATAATTGTTTCAGGTGTTATCTGCATTCATTCATTCATCCTGGACTGAATGTGGCTTCTATTTTGCATAATTTTCCACTCTATTTCCA
TCAAAAGAAGAGGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCCTAGGAAACAATGGAAGAAAATTGAAATCTCCTTCGC



Intron 3 GTAAGTTTCT ... CTATTTTTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A137.392510.120
A264.6227.50
A325.55113.64
A417.26177.10
A522.22832
A628.784010.422
A718.34668.921
A824.9412726.658
A920.368820.525
A1032.395411.840



>TRINITY_DN121464_c1_g1_i2 from sample A3 / Example spliced isoform
CCTTTGCATAGCTTGATACCTACACAAAGAAACAGACATGTTAGTCCTCAATTCCATTAATAATCAACATCAAAGGCAGCCTTATCAAGGTCTGAAGTCAACTTTAACAGAGATATGAAAACTACCTTATTACAGTCTGAAGTTTTGTTTACTTGTATGAGCAAAGCACTAAAGATTGAAGATTACATTGCATGCATGGT
ATGAATGCCAATCAACAGTTTCAGATTTAACTTAGAAACAAGTTGGTCATAAACAATGCAATTCAATTGCAATTTTAGATGAACAGATGCTAAACGCTAATGTAATTGACGTCTTGGCAGCTCTTTCTTGTGTTTTAACATATGTTATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATT
CCGATTGCATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCACTTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGGAAATTGCTTTTTTCTCTGATAATTGTTTCAGGTGTTATCTGCATTCATTCA
TTCATCCTGGACTGAATGTGGCTTCTATTTTGCATAATTTTCCACTCTATTTCCATCAAAAGAAGAGGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCCTAGGAAACAATGGAAGAAAATTGAAATCTCCTTCGCATTCTTGTCTGTCTGTGGATATTCATGCTATTGA
AAAAGAGAAAGGTCGAATGGTTCCAAATTGTTATTATCTTTCTGAACTTGTCAATAAAGAATTTT


>TRINITY_DN121464_c1_g1_i5 from sample A3 / Example unspliced isoform:
AACCAAGCGAACCAGCATAAGGGGTTTTGGGCCGATACTTTGTCCACGTGACCAACCAGCAAAAACTTGTTGGGGAAGCCTGCCCAATTGAAGCCACAGTCCAAACAAACTAAACGTACGCCTCAGTATCAAAATTTAGAACACGCAAGACTTTCATCGGAGACATTCATTGGAGGCGACCATAAATGACAATTTCTTGG
CCTTGAAGCAATCGTTCATCGTTAAATTTGTTCCGATTCTTCGTTGCAGAGCTCAGATCCCTCCCGTTGACTCTTTGAATTAAAGCGCACTGCAAAAAGGCCGGACATTTATTTGCAATCGAAGATGCATCTTTACAATGCTTGGTTGCCTCCTCCGGTGGCGGAGCTTGCCGTTAAAGAAAAGGACGCTTTTACGGAAG
TAGTGAACACAGTAAAAGATGCATGGAAGCCTGACGATCCTGGCTCTGCATATGCTACTCTCAAGTGGATTCCGATCTTGCACAAGCATCGGAGTGCTGATGGTTTAAGGTTGAAGCATAGTCACTTGGAGACAATGATATACCTCATTTATTCTTGTCGGCAGTTTTTCTCATCAGGCTCTGCAGCAGAAATATGGATA
GAATTCAGGCCTTTTATGGAGAATTTATGGCATAACTCGTCACTGGAAGCTGCTGGTTTTGTTCAGTTGTTTCTTCCAACAAATGCAGTAAACCTGGACTTTTTTAATTGTGACTGGATGACAGATTGCTTGAACCTATGGGTTGGAATGCCAAATTGTCAATACTGGGATTTTCAATGGGCTGCTGTCATGGCACGTTG
TATAAAACACTCTATGACAATCGAATGGGAGGGTTTTCTTCCAGTGCTTTTCACACAATACTTGAATATGTTCGAGGTACCGGTGGCAAATAACAGTGGGCCATACCCTTTTTCAAGAGATGATGTTCCGAGAAGTACCATATACGCATTTTCTCAAAGCAAGCTAGTTACACCTTCAAAAGAGATTGCTAAATCAATTG
TATATCTGCTCAAACCTGGTGGTTCGGCTCAAGCTTACCTTGAAAAATTGGTTGACCTTCTAGAACAATATTATCATCCATCTAATGGGGGTCGGTGGAGTTACTCTTTAGAACGCTTTTTGCGATATCTGGTGTACTACTTTCAGAAACGCCTTGCCCAAGAGCAACGAAGTAGAGAGCTTGGAACAGGCATGGTTCAA
AACTTCCTTGGAAGCTCAGAAAGGGAAGCCTTTGTAAGATCTGTTTTGAAATTGATTGAGCGTGGTCAGTACAGCAAGAGTGAATCCCTTGCTGGAACTGCTGCTGTTGCATCTTCTGTGTTATCCTATGTGGAGCCCACTGTTGTTCTGCCCTTTATTGTGTCAAGCTTCCATATTGCACTTGACAATGTAATAGCCCC
ACATCAATTTAAGTCTGCAATCACAGGAATGGCGCTTGCCAGTCGTGCTTTACTTCTAGCTTCTGCAGAAAGTTCTTATTTGGAAACTGACAAATCTGAATGTAATATTTCTCCTACTGGTTACAACCATGCCTTAATGGTTTCCATGTGCAATACTTTACCTGGTATGGATGTCAATGATCCTCCGAAAACCCTTGCAA
CTATGCAGCTCTTTGGATCAATATTCTCTAATCTAGCTGAGGTTGGTGATAAAGAAGATGGCAGCACACCTATTCTGGGTATCGATTTATCTGAATGGCTGGATGAATTCTTTTGTCGTCTTTTTTCTCTACTTATACATTTGGAACCAAACAATCAATCAAATGAAGGACAATTTTTATCAACATCTTCAGGAACTTTC
CTTGTTGAAGATGGACCTTACTATTTTTGTATGCTGGAAATTTTACTTGGTAAACTCACAAGACCTCTTTACCATCAGGCATTGAAAAAGATTTCCAGGTTTGTTCATGGTCATACTCTCCCGGGAGCAGTTGATGAAATTGGATTACTGTGTTCTGCTACATTATATTCTAATCCAGCAGAGGCATTAGTGCACCTGCT
TCAGCCCATGATAAACTCTGTTATATCTTCTCTTAAAGAATCTCCATCGACTGGATGTAGTAGTCGTGAAATTTCATACGCATCATCTGTAACAGAGGCATCATTATCTCCAGCTTTGGAAGCATCGGTAGCATACCAATTGAATGTTCTTTCTGTTGCCATCAACTTTGGGGGTCCACATATTCTTCAGTGCAAAGAAG
AAATAAAAGAAGCTATTTCAGCTGCTTTTGATGCACCATCTTGGAAGGTGAATGAGGCTGGAAACCATCTTTTGAGGTCCCTTATTGGAAGCCTATCTCTTTACTACCCACTGGACCAGTATAAGTGCTATTCTCGTTATCCTGGCATTGATGGGCTAGAGAAATGGATTAGCACGAAGGAAAATCATAATGAAGAAGTT
TCAGTGGGCCCTAAATGGCATATTCCAAATAAAGATGAGATAGCATTTGCGAATGAGCTCCTGGACCTGAATCTGAGGTCAGCTTTGGACAAGCTTAAGCAAATCTGTCAATCACAGCATCCCTTAGAATCAGGATATGAAAAGCAGCTCCTGAGAGTCATTCTTTTGCAAATTGATGCATCACTTCAAGGCGCTCGGTC
TTGTTTACCTGATTTTCACCCACAACAAGGAGATGCAAAACATGATGATGAGAAATACAGCACCTCATATATTTCGGGAGCATCTGGTGTATGTATTGGCAGTACTGAACTAAGGCAAGAGGCTGCAGATGTAGTAGATTTTGCCTGCAAATATATGTTGGAGCATAGAGCAGACAATAGCATATTGCTGATGCTTCTTG
TTCGTATTTTGGATGCATTGGTGAACTATGGGAGCTTGGAATATGATGAGTGGTCAGACCACAAACATGCATGGACAATGGACTCATCCGCAATAAAAGAGCCTCCATCAAACTTTATAACTGGTCATCATGCTCAAGGAAAGCGGAGACCTAGGTGGACACTCATAGATAGATCATACATGCATAATACATGGCGAGCA
TCACAATCAAGTTACCATCAGTTTCGCATTGATAGCAGCATCCGCCCACCAGAGCATATCATTCTTTTGATGAATGATCTTTTGGAACTTTCCTTTAATGACTACGAGGCAGTTCGTCTCCGTGCAGGGAAGTCACTGGTGAAGATGCTGAAGCGATTCCCATCTCTAGTTAAAGATTGTATGCCAAAACTCACGACTAG
CTTAGGAACTCCAGGAATTTCTGAAAATGCTGCCTTGGGGGCTTGTACAGTACTTAAATCCAGAACTGTAATGAGGCACATAATGAAGGATTGGGATGCTTTTTCATCTTTCGTCTTGGCTATACTTGGAAGTTCCCATCAAGGATCATTGAGAGCTCAAAAATCCATTAATGAGCTCTTTCTTGTGTTTAACATACGTT
ATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATTCCGATTGCATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTGGCACTTCCTTCAAATAATAATTTTGATTTGCCATCAAAAACA
AGAGAGGAAATTGCTTgtaagtttcttttccctgtaagtattgtagagtgaaaagtaatactccaaaacataatcttttttatcggcgatgctgaccatgctatttttagTTTTCTCTGATAATTGTTTCAGGTGTTATCTGCATTCATTCATTCATCCTGGACTGAATGTGGCTTCTATTTTGCATAATTTTCCACTCT
ATTTCCATCAAAAGAAGAGGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCCTAGGAAACAATGGAAGAAAATTGAAATCTCCTTCGCATTCTTGTCTGTCTGTGGATATTCATGCTATTGAAAAAGAGAAAGGTCGAATGGTTCCAAATTGTTATTATCTTTCTGAACT
TGTCAATAAAGAATTTT



Intron 4 GTAAGTTTCT ... ATTGTTTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A140.48251420
A246.67895
A3301448
A4201881
A533.33862
A632.33451429
A724.27781634
A829.741373581
A925.4943133
A1034.88561347



>TRINITY_DN48427_c0_g1_i9 from sample A10 / Example spliced isoform
ACTGATTCCAAGAGGCCAATTTTTTAATTTAATTGGACTGAATTCAGTATCTTATAGGGAGGCTGTGGACATAGCAGCAGCAGAATTTATTTGCTAAAGGGAATCATTCTGATGGAGGGCCAAATTCATTATATGTCCATTCTATGTGGAGAGAACCGAAATAGTTGGGGGTCAATTTGCTCAGGATTTGGCGTTAAGGA
AAAACCTGCATCAGAGAAGGAAGATGCTCTTGCTGCCACACCCGACGATGATAAACCCACAATCCCTTAAGCCCTAAGACTCTCTGTCTCAATCTGGCTTTAGGGTTTAGAACTTTCAGCCGACTCTACGAAAGAAGTATAAAAAGGGCAAATGAGGGGTATTCTCCTCATTCAATTCTTTCATTTTGGAGAAGAAAGCT
TGGAGAGCTCTTTGAGTATTTGAGCTAGGGTTTGAGAAAACCCCTACTTGTTGCGACTGGTTCGGGGATGAAAGATTCCTCCCTAGCTAGTGTGGGTGGATTCATCCAGGATTCACACTTGTACTTCAATGCCTTCGTATATTATCTTCGATTCCAGTCAGATCTGAAGCATTTTGTGGCTAGGGTAGGCTAGGGCGCAT
TTGTGGAGAAATATTCTCAGTCTGGTTTGGATGGCGCACTTGGAGAGGAACACCATTGCTTGTGAAGGGCGCATTACATATGCTGATAGGGGCGCACTCGAAGTCTGCTTTGTTCAAGTTTGAGAAGGATGCACTTGAAGATTGTCTTCATGCTTGGTGGAGGGCGCATTCTCAAAAACGGATTATTCTTATTAATGATC
ATTCTTATTAACTGTGATATCTAAGTATAAGAAGGGCCCATCTCTTGACCATTAACCAAATCGTGAAGGAGTCTTCTGCTGCACAAAATCGTAGTCAATAGTGTCCAAACCATTTGCAAAGAAGAGAACAAGTTTCCCCATTGTTCTGCGTCTGCAATCACAGTGGAATAACATCTGGTCCAAACACGGACACTGTGGCT
GTTTGAAGGGCGAGTTCTGTAGTAACTCTTGCAATCTTGAAGATCAAAATCCAATAGTTTCATGCCAAACAGAGAACAAATTCTGTACAGCAGTCTGCCATTGAATCTATTAGCCAGGAATGCGTGGGCCTTCTGTACCATCCAGAAATCTGTAGCAGCATCTCCTTGTTGTTGGGTCAACACACCTCAAAGGCATATAT
GAATGCCTACAGCTCTTTCTTGTGTTTTAACATATGTTATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATTCCGATTACATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCAC
TTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGGAAATTGCTTGTGTTATCTGCATTCATTCATTCATCCTGGACTGAATGTGGCTTCTATTTTGCATAATTTTCCACTCTATTTCCATCAAAAGAAGAGGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCC
TAGGAAACAATGGAAGAAAATTGAAATCTCCTTCGCATTCTTGTCTGTCTGTGGATATTCATGCTATTGAAAAAGAGAAAGGTCGAATGGTTCCAAATTGTTATTATCTTTCTGAACTTGTCAATAAAGAATTTTTCTC


>TRINITY_DN48427_c0_g1_i12 from sample A10 / Example unspliced isoform:
ACTGATTCCAAGAGGCCAATTTTTTAATTTAATTGGACTGAATTCAGTATCTTATAGGGAGGCTGTGGACATAGCAGCAGCAGAATTTATTTGCTAAAGGGAATCATTCTGATGGAGGGCCAAATTCATTATATGTCCATTCTATGTGGAGAGAACCGAAATAGTTGGGGGTCAATTTGCTCAGGATTTGGCGTTAAGGA
AAAACCTGCATCAGAGAAGGAAGATGCTCTTGCTGCCACACCCGACGATGATAAACCCACAATCCCTTAAGCCCTAAGACTCTCTGTCTCAATCTGGCTTTAGGGTTTAGAACTTTCAGCCGACTCTACGAAAGAAGTATAAAAAGGGCAAATGAGGGGTATTCTCCTCATTCAATTCTTTCATTTTGGAGAAGAAAGCT
TGGAGAGCTCTTTGAGTATTTGAGCTAGGGTTTGAGAAAACCCCTACTTGTTGCGACTGGTTCGGGGATGAAAGATTCCTCCCTAGCTAGTGTGGGTGGATTCATCCAGGATTCACACTTGTACTTCAATGCCTTCGTATATTATCTTCGATTCCAGTCAGATCTGAAGCATTTTGTGGCTAGGGTAGGCTAGGGCGCAT
TTGTGGAGAAATATTCTCAGTCTGGTTTGGATGGCGCACTTGGAGAGGAACACCATTGCTTGTGAAGGGCGCATTACATATGCTGATAGGGGCGCACTCGAAGTCTGCTTTGTTCAAGTTTGAGAAGGATGCACTTGAAGATTGTCTTCATGCTTGGTGGAGGGCGCATTCTCAAAAACGGATTATTCTTATTAATGATC
ATTCTTATTAACTGTGATATCTAAGTATAAGAAGGGCCCATCTCTTGACCATTAACCAAATCGTGAAGGAGTCTTCTGCTGCACAAAATCGTAGTCAATAGTGTCCAAACCATTTGCAAAGAAGAGAACAAGTTTCCCCATTGTTCTGCGTCTGCAATCACAGTGGAATAACATCTGGTCCAAACACGGACACTGTGGCT
GTTTGAAGGGCGAGTTCTGTAGTAACTCTTGCAATCTTGAAGATCAAAATCCAATAGTTTCATGCCAAACAGAGAACAAATTCTGTACAGCAGTCTGCCATTGAATCTATTAGCCAGGAATGCGTGGGCCTTCTGTACCATCCAGAAATCTGTAGCAGCATCTCCTTGTTGTTGGGTCAACACACCTCAAAGGCATATAT
GAATGCCTACAGCTCTTTCTTGTGTTTTAACATATGTTATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATTCCGATTACATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCAC
TTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGGAAATTGCTTgtaagtttctttgccctgtaagtattgtagagtgaaaagtaataccccaaaacataatcttttttatcggcgatgctgaccatgctatttttagttttctctgataattgtttcagGTGTTATCTGCATTCATTCATTCATCCTGGACTG
AATGTGGCTTCTATTTTGCATAATTTTCCACTCTATTTCCATCAAAAGAAGAGGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCCTAGGAAACAATGGAAGAAAATTGAAATCTCCTTCGCATTCTTGTCTGTCTGTGGATATTCATGCTATTGAAAAAGAGAAAGGTC
GAATGGTTCCAAATTGTTATTATCTTTCTGAACTTGTCAATAAAGAATTTTTCTC



Intron 5 GCATGTAAAC ... GTCTTGGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A127.4532024
A220.6923610
A363.8317306
A448.4817162
A555.56450
A658.58311711
A742.471269325
A854.7117320942
A952.3613114429
A1046.31806925



>TRINITY_DN48367_c0_g1_i7 from sample A7 / Example spliced isoform
AAGGAACACCATTGCTTGTTAAAGGTGCATTACATCTGCTGATAGGGGTGCACTCGGAGTCTGCTTTGTTCAAGTTTGAGAAGGATGCACTTGAAGATTGTCTTCATGCTTGGTGGAGGGCGCATTCTCAAAAAGGATTATTCTTATTAATTGTGATATCTAAGTATAAGAAGGGCCCATCTCTTGACTATTAACCAAAT
CGTGAAGGAGTCTTCTGCTGCACAAAATCGTAGTCAATAGTGTCCAAACCATTTGCAAAGAAGAGAACAAGTTTCCCCATTGTTCTGCATCTGCAATCACAGTGGAATAACATCTGGTCCAAACACGGACACTGTGGCTGTTTGAAGGGCGAGTTCTGTAGTAACTCTTGCAATCTTGAAGATCGAAATCCAATAGTTTC
ATGCCAAACAGAGAACAAATTCTGTACAGCAGTCTGCCATTGAATCTATTAGCCAGGAATGCGTGGGCCTTCTGTACCATCCAGAAATCTGTAGCAGCATCTCCTTGTTGTTGGGTCAACACACCTCAAAGGCATATATGAATGCCTACAGCTCTTTCTTGTGTTTTAACATATGTTATACTGGGATACCAAGCATCAAA
TATCAATTTTCTGGGGAGGCATTTGACAATTCCGATTACATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCG


>TRINITY_DN48367_c0_g1_i3 from sample A7 / Example unspliced isoform:
AAGGAACACCATTGCTTGTTAAAGGTGCATTACATCTGCTGATAGGGGTGCACTCGGAGTCTGCTTTGTTCAAGTTTGAGAAGGATGCACTTGAAGATTGTCTTCATGCTTGGTGGAGGGCGCATTCTCAAAAAGGATTATTCTTATTAATTGTGATATCTAAGTATAAGAAGGGCCCATCTCTTGACTATTAACCAAAT
CGTGAAGGAGTCTTCTGCTGCACAAAATCGTAGTCAATAGTGTCCAAACCATTTGCAAAGAAGAGAACAAGTTTCCCCATTGTTCTGCATCTGCAATCACAGTGGAATAACATCTGGTCCAAACACGGACACTGTGGCTGTTTGAAGGGCGAGTTCTGTAGTAACTCTTGCAATCTTGAAGATCGAAATCCAATAGTTTC
ATGCCAAACAGAGAACAAATTCTGTACAGCAGTCTGCCATTGAATCTATTAGCCAGGAATGCGTGGGCCTTCTGTACCATCCAGAAATCTGTAGCAGCATCTCCTTGTTGTTGGGTCAACACACCTCAAAGGCATATATGAATGCCTACAGgcatgtaaacattgctattttaatgaatatacagatctgatatttttct
attaaatctgagattgtattagaattgctactgtatgttgaatacaacttctctttatacgaaatttcaaacaaacaccaacacacctaagtcagaagaatgttcctagaattggctattgaataggcagctacagcaacaacatacaaagtgtttgtcaaaactcctacctgaatctcaaatagcaagggtgtacggtc
atcagttttaacaagggagtttcaacctacagcaattcatgtgtttgaaacttgtcgacatcattcaaacaactcatctattacgattgggtacctagcttgtgccacttggctatactatagcagtcccactaaggtaaatttcctgtgacgacaaaattctttgccacgattgggtacctagcttgtgccacttggct
atactatagcagtcccactaaggtaaatttcctgtgacgacaaaattctttgccaacttcacaagttgttaggaaacaaagttagttcagattgtcttggaatactacagtggtatcagagcatgaatctcaacagctgtggggttttgatgaaaggtaaacgaattgctacaggagttattgagtatatttggcagtgt
tatctacagcggaacttgcctttccaagacaccttcactttgatattgacaacactgttatactattgttggggtcaccatttacagtcgtagaattactatgcttgaaattagatagattggttaggtgttagtaattatcttggaacaatttagggatgcaacagcctccaaacaggggcccttttccacaatttgta
agcctgctagggaatcatatttttctcaccacaaactcctcctttgcatagcttgatacctacacaaagaaacagacatgttagtcctcaattccattaataatcaacatcaaaggcagccttatcaaggtctgaagtcaactttaacagagatatgaaaactaccttattacagtctgaagttttgtttacttgtatga
gcaaagcactaaagattaaagattacattgcatgcatggtatgactgccaatcaacagtttcagatttaacttagaaacaagttggtaataaacaatgcaattcaattgcaattttagatgaacagatgctaaacgctaatgtaattgacgtcttggcagCTCTTTCTTGTGTTTTAACATATGTTATACTGGGATACCA
AGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATTCCGATTACATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCACTTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGGAAATTGCTTGT
AAGTTTCTTTGCCCTGTAAGTATTGTAGAGTGAAAAGTAATACCCCAAAACATAATCTTTTTTATCGGCGATGCTGACCATGCTATTTTTAGTTTTCTCTGATAATTGTTTCAGGTGTTATCTGCATTCATTCATTCATCCTGGACTGAATGTGGCTTCTATTTTGCATAATTTTCCACTCTATTTCCATCAAAAGAAGA
GGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCCTAGGAAACAATGGAAGAAAATTGAAATCTCCTTCGC



Intron 6 GTATAGTATT ... GCTTTTCTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A123.248821
A222.3340149
A32036513
A424.0741620
A521.6229610
A623.08512
A725.4919013
A814.29301
A920804
A1018.18922



>TRINITY_DN105448_c7_g1_i1 from sample A4 / Example spliced isoform
AGGCCTTTTATGGAGAATTTATGGCATAACTCGTCACTGGAAGCTGCTGGTTTTGTTCAGTTGTTTCTTCCAACAAATGCAGTAAACCTGGACTTTTTTAATTGTGACTGGATGACAGATTGCTTGAACCTATGGGTTGGAATGCCAAATTGTCAATACTGGGATTTTCAATGGGCTGCTGTCATGGCACGTTGTATAAA
ACACTCTATGACAATCGAATGGGAGGGTTTTCTTCCAGTGCTTTTCACACAATACTTGAATATGTTCGAGGTACCGGTGGCAAATAACAGTGGGCCATACCCTTTTTCAAGAGATGATGTTCCGAGAAGTACCATATACGCATTTTCTCAAAGCAAGCTAGTTACACCTTCAAAAGAGATTGCTAAATCAATTGTATATC
TGCTCAAACCTGGTGGTTCGGCTCAAGCTTACCTTGAAAAATTGGTTGACCTTCTAGAACAATATTATCATCCATCTAATGGGGGTCGGTGGAGTTACTCTTTAGAACGCTTTTTGCGATATCTGGTGTACTACTTTCAGAAACGCCTTGCCCAAGAGCAACGAAGTAGAGAGCTTGGAACAGGCATGGTTCAAAACTTC
CTTGGAAGCTCAGAAAGGGAAGCCTTTGTAAGATCTGTTTTGAAATTGATTGAGCGTGGTCAGTACAGCAAGAGTGAATCCCTTGCTGGAACTGCTGCTGTTGCATCTTCTGTGTTATCCTATGTGGAGCCCACTGTTGTTCTGCCCTTTATTGTGTCAAGCTTCCATATTGCACTTGACAATGTAATAGCCCCACATCA
ATTTAAGTCTGCAATCACAGGAATGGCGCTTGCCAGTCGTGCTTTACTTCTAGCTTCTGCAGAAAGTTCTTATTTGGAAACTGACAAATCTGAATGTAATATTTCTCCTACTGGTTACAACCATGCCTTAATGGTTTCCATGTGCAATACTTTACCTGGTATGGATGTCAATGATCCTCCGAAAACCCTTGCAACTATGC
AGCTCTTTGGATCAATATTCTCTAATCTAGCTGAGGTTGGTGATAAAGAAGATGGCAGCACACCTATTCTGGGTATCGATTTATCTGAATGGCTGGATGAATTCTTTTGTCGTCTTTTTTCTCTACTTATACATTTGGAACCAAACAATCAATCAAATGAAGGACAATTTTTATCAACATCTTCAGGAACTTTCCTTGTT
GAAGATGGACCTTACTATTTTTGTATGCTGGAAATTTTACTTGGTAAACTCACAAGACCTCTTTACCATCAGGCATTGAAAAAGATTTCCAGGTTTGTTCATGGTCATACTCTCCCGGGAGCAGTTGATGAAATTGGATTACTGTGTTCTGCTACATTATATTCTAATCCAGCAGAGGCATTAGTGCACCTGCTTCAGCC
CATGATAAACTCTGTTATATCTTCTCTTAAAGAATCTCCATCGACTGGATGTAGTAGTCGTGAAATTTCATACGCATCATCTGTAACAGAGGCATCATTATCTCCAGCTTTGGAAGCATCGGTAGCATACCAATTGAATGTTCTTTCTGTTGCCATCAACTTTGGGGGTCCACATATTCTTCAGTGCAAAGAAGAAATAA
AAGAAGCTATTTCAGCTGCTTTTGATGCACCATCTTGGAAGGTGAATGAGGCTGGAAACCATCTTTTGAGGTCCCTTATTGGAAGCCTATCTCTTTACTACCCACTGGACCAGTATAAGTGCTATTCTCGTTATCCTGGCATTGATGGGCTAGAGAAATGGATTAGCACGAAGGAAAATCATAATGAAGAAGTTTCAGTG
GGCCCTAAATGGCATATTCCAAATAAAGATGAGATAGCATTTGCGAATGAGCTCCTGGACCTGAATCTGAGGTCAGCTTTGGACAAGCTTAAGCAAATCTGTCAATCACAGCATCCCTTAGAATCAGGATATGAAAAGCAGCTCCTGAGAGTCATTCTTTTGCAAATTGATGCATCACTTCAAGGTGCTCGGTCTTGTTT
ACCTGATTTTCACCCACAACAAGGAGATGCAAAACATGATGATGAGAAATACAGCACCTCATATATTTCGGGAGCATCTGGTGTATGTATTGGCAGTACTGAACTAAGGCAAGAGGCTGCAGATGTAGTAGATTTTGCCTGCAAATATATGTTGGAGCATAGAGCAGACAATAGCATATTGCTGATGCTTCTTGTTCGTA
TTTTGGATGCATTGGTGAACTATGGGAGCTTGGAATATGATGAGTGGTCAGACCACAAACATGCATGGACAATGGACTCATCCGCAATAAAAGAGCCTCCATCAAACTTTATAACTGGTCATCATGCTCAAGGAAAGCGGAGACCTAGGTGGGCACTCATAGATAGATCATACATGCATAATACATGGCGAGCATCACAA
TCAAGTTACCATCAGTTTCGCATTGATAGCAGCATCCGCCCACCAGAGCATATCATTCTTTTGATGAATGATCTTTTGGAACTTTCCTTTAATGACTACGAGGCAGTTCGTCTCCGTGCAGGGAAGTCACTGGTGAAGATGCTGAAGCGATTCCCATCTCTAGTTAAAGATTGTATGCCAAAACTCACGACTAGCTTAGG
AACTCCAGGAATTTCTGAAAATGCTGCCTTGGGGGCTTGTACAGTACTTAAATCCAGAACTGTAATGAGGCACATAATGAAGGATTGGGATGCTTTTTCATCTTTCGTCTTGGCTATACTTGGAAGTTCCCATCAAGGATCATTGAGAGCTCAAAAATCCATTAATGAGCTCTTTCTTGTGTTTAACATACGTTATACTG
GGATACCAAGCATCAAATATCAATTTTCTGGGGAGACATTTGACAATTCCGATTGCATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCACTTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGGAAA
TTGCTTTTTTCTCTGATAATTGTTTCAGGTGTTATCTGCATTCATTCATTCATCCTGGACTGAATGTGGCTTCTATTTTGCATAATTTTCCACTCTATTTCCATCAAAAGAAGAGGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCCTAGGAAACAATGGAAGAAAATT
GAAATCTCCTTCGCATTCTTGTCTGTCTGTGGATATTCATGCTATTGAAAAAGAGAAAGGTCGAATGGTTCC


>TRINITY_DN105448_c7_g1_i4 from sample A4 / Example unspliced isoform:
ATATGAAAAGCAGCTCCTGAGAGTCATTCTTTTGCAAATTGATGCATCACTTCAAGGTGCTCGGTCTTGTTTACCTGATTTTCACCCACAACAAGGAGATGCAAAACATGATGATGAGAAATACAGCACCTCATATATTTCGGGAGCATCTGGTGTATGTATTGGCAGTACTGAACTAAGGCAAGAGGCTGCAGATGTAG
TAGATTTTGCCTGCAAATATATGTTGGAGCATAGAGCAGACAATAGCATATTGCTGATGCTTCTTGTTCGTATTTTGGATGCATTGGTGAACTATGgtatagtattgtacctttaactatgggtttcacatagactgaactggagccttgttgccagctgcaaattgttttatcatctagcatttttgaagtttatatgt
tgatacttatgattcaatgagcttttctagGGAGCTTGGAATATGATGAGTGGTCAGACCACAAACATGCATGGACAATGGACTCATCCGCAATAAAAGAGCCTCCATCAAACTTTATAACTGGTCATCATGCTCAAGGAAAGCGGAGACCTAGGTGGGCACTCATAGATAGATCATACATGCATAATACATGGCGAGCA
TCACAATCAAGTTACCATCAGTTTCGCATTGATAGCAGCATCCGCCCACCAGAGCATATCATTCTTTTGATGAATGATCTTTTGGAACTTTCCTTTAATGACTACGAGGCAGTTCGTCTCCGTGCAGGGAAGTCACTGGTGAAGATGCTGAAGCGATTCCCATCTCTAGTTAAAGATTGTATGCCAAAACTCACGACTAG
CTTAGGAACTCCAGGAATTTCTGAAAATGCTGCCTTGGGGGCTTGTACAGTACTTAAATCCAGAACTGTAATGAGGCACATAATGAAGGATTGGGATGCTTTTTCATCTTTCGTCTTGGCTATACTTGGAAGTTCCCATCAAGGATCATTGAGAGCTCAAAAATCCATTAATGAGCTCTTTCTTGTGTTTAACATACGTT
ATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGACATTTGACAATTCCGATTGCATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCACTTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAG
AGGAAATTGCTTGTAAGTTTCTTTGCCCTGTAAGTATTGTAGAGTGAAAAGTAATACCCCAAAACATAATCTTTTTTATCGGCGATGCTGAC



Intron 7 GTAACATTTT ... TTGTATTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A135.88423017
A226.2452289
A339.6453128
A428.77552222.4
A536.5120149
A618.2111223.926
A714.6213.650.622.4
A820.8152.662.717.4
A917.5212.150.739.3
A1020.6189.163.135



>TRINITY_DN46149_c0_g1_i2 from sample A8 / Example spliced isoform
CGATGATTAGAGGCACTCTACAATCTCAACCACGCTCCAAAGCAGATTGGTGTAGAAGAGGGAAAACTAAAATATTAATTTAGGGCTATATTTCAAAAGATTTTGAATTTGGGACTTTCCCCAACAGGCAGCTTTCCAAAAGGCTTGCAGTTGCATTAGCAGGTGTGAGGTGACCATTGATCTGGTAGGAGTAAGCAAAG
CAGGGGGAGGGAAGCAAATCATCTCTGGTGTTGTCTTTCTGTATCATATTTATGGGCAGGGGGTCGACATTGATCGATTATAATGGAGACATTATCAACAGAGGAGAAAGATTTACAATGCATATTCAACCTCAAGAACAAAGTAGCTCTCAAGTTCAAATCCCATCAGTGTTTTTTTCATGAAGTGTTGTTATCCAATG
TGTTATCCCATCAGTCGAATTTATTTGATAAAAGGAATCGTTCTGATGGAGAGCCGAATTCATTCTATGGCCATTCTGTGTGGAGAGAACCGAAATAGTTGGGGGTCGATTTGGTCAGGATTTGGCGTTAAGGAAAAACCTGCATCAGAGAAGGAAGATGCTCTTGCTGCCACACCCGACGATGATAAACCCACAATCCC
TAAAGCCCTAGGACTCTCTGTCTCAATCTGGATTCTTCAGGGTTTAGAACTTTCAGCCGACTCTACGAAAGAAGTATAAAAAGGGCAAATGAGGGGTATTCTCCTCATTCAACACTTTCATTTTGGGGAAGAAAGCTTGGAGAGCTCTTTGAGTATTTGAGCTAGGGTTTGAGAAAACCCTTGCTTGTTGCGACTGGTTC
GGGGGTGAAATATTCCTCCCTAGCTGGTGTGGGTGGATTCATCCAGGATTCACACTTGTACTTCATTGCCATCGTATATTATCTTCGATTCCAGTCATATTTGAAGCATTTTGAGGCTAGGGCGCATTTGTGGAGAAATATTCTCAGTCTGGTTTGGATGGCGCACTTGGAGAGGAAGGAACACCATTGCTTGTTAAAGG
TGCATTACATCTGCTGATAGGGGTGCACTCGGAGTCTGCTTTGTTCATGTTTGAGAAGGATGCACTTGAAGATTGTCTTCATGCTTGGTGGAGGGCGCATTCTAAAAAAGGATCATTCTTATTAACTGTGATATCTAAGTATAAGAAGGGCCCATCTCTTGACTATTAACCAAATCGTGAAGGAGTCTTCTACTACACAA
AATCGTAGTCAATAGTGTCCAAACCATTTGCAAAGAAGAGAACAAGTTTCCCCATTGTTCTGCGTCTGCAATCACAGTGGAATAACATCTGGTCGAAACACGAACACTATGGTTGTTTGAAGGGCGAGTTTTGTAGTAACTCTTGCAATCTTGAAGATCGAAATCCAATAGTTTCATGCCAAACAAAGAACAAATTTTGT
ACAACATTCTGCCATTGAATCTATTAGCCAGGAATGTGTGGGCCTCCTGTACCATCCAGAAATCTGTAGCAGCATCTCCTTGTTGTTGGGTCAACACACCTCAAAGGCATATATGAATTCCTGCAGGCATGTAAACATTGCTATTTTAATGAATATACAGATCTGATATTTTTCTATTAAATCTGAGATTGTATTAGAAT
TGCTACTGTATGTTGAATACAACTTCTCTTTATACGAAATTTCAAACAAACACCAACACACCTAAGTCAGAAGAATGTTCCTAGAATTGGCTATTGAATAGGCAGCTACAGCAACAACATACAAAGTGTTTGTCAAAACTCCTACCTGAATCTCAAATAGCAAGGGTGTACGGTCATCAGTTTTAACAAGGGAGTTTCAA
CCTACAGCAATTCATGTGTTTGAAACTTGTCGACATCATTCAAACAACTCATCTATTACGATTGGGTACCTAGCTTGTGCCACTTGGCTATACTATAGCAGTCCCACTAAGGTAAATTTCCTGTGACGACAAAATTCTTTGCCACGATTGGGTACCTAGCTTGTGCCACTTGGCTATACTATAGCAGTCCCACTAAGGTA
AATTTCCTGTGACGACAAAATTCTTTGCCAACTTCACAAGTTGTTAGGAAACAAAGTTAGTTCAGATTGTCTTGGAATACTACAGTGGTATCAGAGCATGAATCTCAACAGCTGTGGGGTTTTGATGAAAGGTAAACGAATTGCTACAGGAGTTATTGAGTATATTTGGCAGTGTTATCTACAGCGGAACTTGCCTTTCC
AAGACACCTTCACTTTGATATTGACAACACTGTTATACTATTGTTGGGGTCACCATTTACAGTCGTAGAATTACTATGCTTGAAATTAGATAGATTGGTTAGGTGTTAGTAATTATCTTGGAACAATTTAGGGATGCAACAGCCTCCAAACAGGGGCCCTTTTCCACAATTTGTAAGCCTGCTAGGGAATCATATTTTTC
TCACCACAAACTCCTCCTTTGCATAGCTTGATACCTACACAAAGAAACAGACATGTTAGTCCTCAATTCCATTAATAATCAACATCAAAGGCAGCCTTATCAAGGTCTGAAGTCAACTTTAACAGAGATATGAAAACTACCTTATTACAGTCTGAAGTTTTGTTTACTTGTATGAGCAAAGCACTAAAGATTAAAGATTA
CATTGCATGCATGGTATGACTGCCAATCAACAGTTTCAGATTTAACTTAGAAACAAGTTGGTAATAAACAATGCAATTCAATTGCAATTTTAGATGAACAGATGCTAAACGCTAATGTAATTGACGTCTTGGCAGCTCTTTCTTGTGTTTTAACATATGTTATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGG
AGGCATTTGACAATTCCGATTACATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCACTTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGGAAATTGCTTTTTTCTCTGATAATTGTTTCAGGTGTT
ATCTGCATTCATTCATTCATCCTGGACTGAATGTGGCTTCTATTTTGCATAATTTTCCACTCTATTTCCATCAAAAGAAGAGGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCCTAGGAAACAATGGAAGAAAATTGAAATCTCCTTCGCATTCTTGTCTGTCTGTGGA
TATTCATGCTATTGAAAAAGAGAAAGGTCGAATGGTTCCAAATTGTTATTATCTTTCTGAACTTGTCAATAAAGAATTTTTCTCTAA


>TRINITY_DN46149_c0_g1_i3 from sample A8 / Example unspliced isoform:
CGATGATTAGAGGCACTCTACAATCTCAACCACGCTCCAAAGCAGATTGGTGTAGAAGAGGGAAAACTAAAATATTAATTTAGGGCTATATTTCAAAAGATTTTGAATTTGGGACTTTCCCCAACAGGCAGCTTTCCAAAAGGCTTGCAGTTGCATTAGCAGGTGTGAGGTGACCATTGATCTGGTAGGAGTAAGCAAAG
CAGGGGGAGGGAAGCAAATCATCTCTGGTGTTGTCTTTCTGTATCATATTTATGGGCAGGGGGTCGACATTGATCGATTATAATGGAGACATTATCAACAGAGGAGAAAGATTTACAATGCATATTCAACCTCAAGAACAAAGTAGCTCTCAAGTTCAAATCCCATCAGTGTTTTTTTCATGAAGTGTTGTTATCCAATG
TGTTATCCCATCAGTCGAATTTATTTGATAAAAGGAATCGTTCTGATGGAGAGCCGAATTCATTCTATGGCCATTCTGTGTGGAGAGAACCGAAATAGTTGGGGGTCGATTTGGTCAGGATTTGGCGTTAAGGAAAAACCTGCATCAGAGAAGGAAGATGCTCTTGCTGCCACACCCGACGATGATAAACCCACAATgta
acatttttataaccctaacatcttttatttccctgaatgctcacaatggggtttcgaacccttaacagtttcaattttgattggcatctagtttccaatcacatacgcaaattttgttacatgggtttttggtgcagcctagaactgtaataattttatttttttcaattgtattcagCCCTAAAGCCCTAGGACTCTCT
GTCTCAATCTGGATTCTTCAGGGTTTAGAACTTTCAGCCGACTCTACGAAAGAAGTATAAAAAGGGCAAATGAGGGGTATTCTCCTCATTCAACACTTTCATTTTGGGGAAGAAAGCTTGGAGAGCTCTTTGAGTATTTGAGCTAGGGTTTGAGAAAACCCTTGCTTGTTGCGACTGGTTCGGGGGTGAAATATTCCTCC
CTAGCTGGTGTGGGTGGATTCATCCAGGATTCACACTTGTACTTCATTGCCATCGTATATTATCTTCGATTCCAGTCATATTTGAAGCATTTTGAGGCTAGGGCGCATTTGTGGAGAAATATTCTCAGTCTGGTTTGGATGGCGCACTTGGAGAGGAAGGAACACCATTGCTTGTTAAAGGTGCATTACATCTGCTGATA
GGGGTGCACTCGGAGTCTGCTTTGTTCATGTTTGAGAAGGATGCACTTGAAGATTGTCTTCATGCTTGGTGGAGGGCGCATTCTAAAAAAGGATCATTCTTATTAACTGTGATATCTAAGTATAAGAAGGGCCCATCTCTTGACTATTAACCAAATCGTGAAGGAGTCTTCTACTACACAAAATCGTAGTCAATAGTGTC
CAAACCATTTGCAAAGAAGAGAACAAGTTTCCCCATTGTTCTGCGTCTGCAATCACAGTGGAATAACATCTGGTCGAAACACGAACACTATGGTTGTTTGAAGGGCGAGTTTTGTAGTAACTCTTGCAATCTTGAAGATCGAAATCCAATAGTTTCATGCCAAACAAAGAACAAATTTTGTACAACATTCTGCCATTGAA
TCTATTAGCCAGGAATGTGTGGGCCTCCTGTACCATCCAGAAATCTGTAGCAGCATCTCCTTGTTGTTGGGTCAACACACCTCAAAGGCATATATGAATTCCTGCAGGCATGTAAACATTGCTATTTTAATGAATATACAGATCTGATATTTTTCTATTAAATCTGAGATTGTATTAGAATTGCTACTGTATGTTGAATA
CAACTTCTCTTTATACGAAATTTCAAACAAACACCAACACACCTAAGTCAGAAGAATGTTCCTAGAATTGGCTATTGAATAGGCAGCTACAGCAACAACATACAAAGTGTTTGTCAAAACTCCTACCTGAATCTCAAATAGCAAGGGTGTACGGTCATCAGTTTTAACAAGGGAGTTTCAACCTACAGCAATTCATGTGT
TTGAAACTTGTCGACATCATTCAAACAACTCATCTATTACGATTGGGTACCTAGCTTGTGCCACTTGGCTATACTATAGCAGTCCCACTAAGGTAAATTTCCTGTGACGACAAAATTCTTTGCCACGATTGGGTACCTAGCTTGTGCCACTTGGCTATACTATAGCAGTCCCACTAAGGTAAATTTCCTGTGACGACAAA
ATTCTTTGCCAACTTCACAAGTTGTTAGGAAACAAAGTTAGTTCAGATTGTCTTGGAATACTACAGTGGTATCAGAGCATGAATCTCAACAGCTGTGGGGTTTTGATGAAAGGTAAACGAATTGCTACAGGAGTTATTGAGTATATTTGGCAGTGTTATCTACAGCGGAACTTGCCTTTCCAAGACACCTTCACTTTGAT
ATTGACAACACTGTTATACTATTGTTGGGGTCACCATTTACAGTCGTAGAATTACTATGCTTGAAATTAGATAGATTGGTTAGGTGTTAGTAATTATCTTGGAACAATTTAGGGATGCAACAGCCTCCAAACAGGGGCCCTTTTCCACAATTTGTAAGCCTGCTAGGGAATCATATTTTTCTCACCACAAACTCCTCCTT
TGCATAGCTTGATACCTACACAAAGAAACAGACATGTTAGTCCTCAATTCCATTAATAATCAACATCAAAGGCAGCCTTATCAAGGTCTGAAGTCAACTTTAACAGAGATATGAAAACTACCTTATTACAGTCTGAAGTTTTGTTTACTTGTATGAGCAAAGCACTAAAGATTAAAGATTACATTGCATGCATGGTATGA
CTGCCAATCAACAGTTTCAGATTTAACTTAGAAACAAGTTGGTAATAAACAATGCAATTCAATTGCAATTTTAGATGAACAGATGCTAAACGCTAATGTAATTGACGTCTTGGCAGCTCTTTCTTGTGTTTTAACATATGTTATACTGGGATACCAAGCATCAAATATCAATTTTCTGGGGAGGCATTTGACAATTCCGA
TTACATGAATTTGATCACTCAAATAAGATCCTTGATCAGTGATATGCATAGTGTGAATTGGCGATATAATCTGATGGCACATAGGATGCTTCTGCTACTAGCACTTCAAATAATAATTTTGATTTGCCATCAAAAACAAGAGAGGAAATTGCTTTTTTCTCTGATAATTGTTTCAGGTGTTATCTGCATTCATTCATTCA
TCCTGGACTGAATGTGGCTTCTATTTTGCATAATTTTCCACTCTATTTCCATCAAAAGAAGAGGCGGTGTAAGGCTTCAAGTCAATTTTGTTTTAGCATGAGAATATAGAAATGAAGAGATATTCCTAGGAAACAATGGAAGAAAATTGAAATCTCCTTCGCATTCTTGTCTGTCTGTGGATATTCATGCTATTGAAAAA
GAGAAAGGTCGAATGGTTCCAAATTGTTATTATCTTTCTGAACTTGTCAATAAAGAATTTTTCTCTAA