NA ----> NA

Intron 1 GTAATACTTG ... CGGAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.22	17	75	34
A2	58.92	38	79	30
A3	77.63	17	82	36
A4	65.22	24	65	25
A5	76.47	8	27	25
A6	58.95	47	102	33
A7	62.64	65	144	74
A8	68.93	48	132	81
A9	62.63	54	119	62
A10	62.94	73	172	76

>TRINITY_DN43335_c0_g4_i14 from sample A6 / Example spliced isoform

TTGGGAGGAGAAAAGATAGAGCGGAGCCGAGCAGAATTCAGGGAGCATATTTTAGTGTCTGCAGCAGGATGTCGCGGGGAATAGTGGGATTTCATGGTTCTGTGCGTGTCCTCATTGAAGCCGATGGTTCTGGGCGTGTCCTCATTGAAGCTGATGGTTCTGTGCGTGTCCTCCGTGAAGCCGATGGTTCTGAGCCAGAG
CTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTATCGTTGATGCTGCGGAATTTCGGGATTGCAGAGCAATAATCAGGAATCGGTGTTGTCTATGGATTTGACGATTTGGACCGGAAGCTGGCCGCCGTGTTGTCTAAGGATTCGATGATTTGTTTAGTATGGTATTATTGGTGATGGATCTTTGTATCTTGGA
CTGATATGTAATTTTATATATTCCAATGGAAATATAGTATATTTTTTCTGTTGAAAATAGAACTATCTATCACTTGTTGAATTATTAATGTACTTAGAGTTGTTCATTTTGTCTAGAAGTGGATCACCGA

>TRINITY_DN43335_c0_g4_i8 from sample A6 / Example unspliced isoform:

TTGGGAGGAGAAAAGATAGAGCGGAGCCGAGCAGAATTCAGGGAGCATATTTTAGTGTCTGCAGCAGGATGTCGCGGGGAATAGTGGGATTTCATGGCCTACTCCTTGAAGCCGATGGATGGTTCTGGGCGTGTCCTCATTGAAGCTGATGGTTCTGTGCGTGTCCTCCGTGAAGCCGATGGTTCTGgtaatacttgaaa
ttgcaacgcgtggtgtaattgatcagaaccagggccgcgcaacctaattgatagacagatagagaggagcccaggaaaagttagacagatagagaggagccgagcagatttcagggtttgtaagaagcagagatgaagaagagccacatctgctggcttggccagtgccaaaccctgaaaagatgagagagtagagatca
aaatatatgatgagcgtccaataacttttgtgtttgattattgtgtttttctgagggcatggccatgcttattttattttaccttccattctgtgttaatttttttctgtgttgcatttttctttttctctttttatttttattttttataatttttgcattttccttttgggtttgctttgttcgtctctcttttgagt
ttgatttgtttgtgatcatttaagtagtgtgtaaatagctagattgaaatttaatctttattgcattatttcatcatgttctgttttgtcccggagtgcagAGCCAGAGCTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTATCGTTGATGCTGCGGAATTTCGGGATTGCAGAGCAATAATCAGGAATCGGT
GTTGTCTATGGATTTGACGATTTGGACCGGAAGCTGGCCGCCGTGTTGTCTAAGGATTCGATGATTTGTTTAGTATGGTATTATTGGTGATGGATCTTTGTATCTTGGACTGATATGTAATTTTATATATTCCAATGGAAATATAGTATATTTTTTCTGTTGAAAATAGAACTATCTATCACTTGTTGAATTATTAATGT
ACTTAGAGTTGTTCATTTTGTCTAGAAGTGGATCACCGA

Intron 2 GTAATACTTG ... CGGAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.71	17	72	34
A2	60.62	38	87	30
A3	78.48	17	88	36
A4	67.79	24	76	25
A5	78.38	8	33	25
A6	62.7	47	125	33
A7	64.29	65	160	74
A8	68.83	48	131	81
A9	64	54	130	62
A10	64.3	73	187	76

>TRINITY_DN43335_c0_g4_i14 from sample A6 / Example spliced isoform

TTGGGAGGAGAAAAGATAGAGCGGAGCCGAGCAGAATTCAGGGAGCATATTTTAGTGTCTGCAGCAGGATGTCGCGGGGAATAGTGGGATTTCATGGTTCTGTGCGTGTCCTCATTGAAGCCGATGGTTCTGGGCGTGTCCTCATTGAAGCTGATGGTTCTGTGCGTGTCCTCCGTGAAGCCGATGGTTCTGAGCCAGAG
CTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTATCGTTGATGCTGCGGAATTTCGGGATTGCAGAGCAATAATCAGGAATCGGTGTTGTCTATGGATTTGACGATTTGGACCGGAAGCTGGCCGCCGTGTTGTCTAAGGATTCGATGATTTGTTTAGTATGGTATTATTGGTGATGGATCTTTGTATCTTGGA
CTGATATGTAATTTTATATATTCCAATGGAAATATAGTATATTTTTTCTGTTGAAAATAGAACTATCTATCACTTGTTGAATTATTAATGTACTTAGAGTTGTTCATTTTGTCTAGAAGTGGATCACCGA

>TRINITY_DN43335_c0_g4_i16 from sample A6 / Example unspliced isoform:

TTGGGAGGAGAAAAGATAGAGCGGAGCCGAGCAGAATTCAGGGAGCATATTTTAGTGTCTGCAGCAGGATGTCGCGGGGAATAGTGGGATTTCATGGTTCTGTGCGTGTCCTCATTGAAGCCGATGGTTCTGGGCGTGTCCTCATTGAAGCTGATGGTTCTGTGCGTGTCCTCCGTGAAGCCGATGGTTCTGgtaatact
tgaaattgcaacgcgtggtgtaattgatcagaacagatagagaggagcccaggaaaagttagacagatagagaggagccgagcagatttcagggtttgtaagaagcagagatgaagaagagccacatctgctggcttggccagtgccaaaccctgaaaagatgagagagtagagatgaaaatatatgatgagcgtccaat
aacttttgtggtttgattattgtgtttttctgagggcatggccatgcttattttattttaccttccattctgtgttaatttttttctgtgttgcatttttctttttctctttttatttttattttttataatttttgcattttccttttgggtttgctttgttcgtctctcttttgagtttgatttgtttgtgatcattt
aagtagtgtgtaaatagctagattgaaatttaatctttattgcattatttcatcatgttctgttttgtcccggagtgcagAGCCAGAGCTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTATCGTTGATGCTGCGGAATTTCGGGATTGCAGAGCAATAATCAGGAATCGGTGTTGTCTATGGATTTGACGAT
TTGGACCGGAAGCTGGCCGCCGTGTTGTCTAAGGATTCGATGATTTGTTTAGTATGGTATTATTGGTGATGGATCTTTGTATCTTGGACTGATATGTAATTTTATATATTCCAATGGAAATATAGTATATTTTTTCTGTTGAAAATAGAACTATCTATCACTTGTTGAATTATTAATGTACTTAGAGTTGTTCATTTTGT
CTAGAAGTGGATCACCGA

Intron 3 GTAAACAGTT ... ATGTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.58	30	13	16
A2	33.33	21	10	11
A3	29.11	28	10	13
A4	27.27	20	7	8
A5	58.82	7	11	9
A6	7.69	48	1	7
A7	7.09	295	13	32
A8	11.21	289	24	49
A9	7.54	144	4.5	19
A10	6.22	245	1.5	31

>TRINITY_DN124718_c5_g1_i8 from sample A2 / Example spliced isoform

TGGGTTTGCTTTATTCGTCTCTGTTTTGAGTTTGATTTGTTTGGGATCGAGAGGGAGGATGGAGGACTAAAAATATCCAGAAGTGGGTATCGCTGGGTTGTCATCCGCCAAGGCGGAGAAGAGCCAGAGCTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTACTCGGGGTTGCAGCTAGGTGCAGAAATCGTT
GATGCTGCTCGGGGTTGCAGGTGGGTTTTTAAGAAGAATAATCAGGTGCAGAAATCGTTGATGCCGCGGAATTTCGGGATTGCAGAGCAATAATCAGGTGAGGTGGAGAAGAATGCAGGCTAGTTTGAAGTGCCTTCAGAGAAATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAAAGTGCAGGCTAGTTTGAAGT
GCCTTTAGTGAAATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAGAGTGTGCAAGCTATTTTGAAGTGCCTCCACAAAAATATAAGGAAAAGGTGGGTGGATGAAGAGTGCAAGTTATTTTTGCATTGATAGAAAATTTGAAAGCGCTTTGCAGTAGACTAATAATAGAAGATGTAATTAGATAAGGAAAAGGTGG
GTGGATGAAGAGTGCAAGTTATTTTTGCATTGATAGAAAATTTGAAAGCGCTTTGCAGTAGA

>TRINITY_DN124718_c5_g1_i12 from sample A2 / Example unspliced isoform:

CGCGGAATTTCGGGATTGCAGAGCAATAATCAGGTGAGGTGGAGAAGAATGCAGGCTAGTTTGAAGTGCCTTCAGTATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAAAGTGCAGGCTAGTTTGAAGTGCCTTGAAATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAGAGTGCAAGCTATTTTGAAGTGCCT
CCACAAAAATATAAGGAAAAGGTAAACAGTTTTTATTTTGATTAGATTTTAAATTTATCAAAAATATAAGGAAAAGgtaaacagtttttattttgattagattttaaatttattggtaccattttaaaaaattaaatttgtataaattaatcatatttagacttttttattatttttataaatttaaaatatttttgatg
tgtacagGTGGGTGGATGAAGAGTGCAAGTTATTTTTGCATTGATAGAAAATTTGAAAGCGCTTTGCAGTAGACTAATAATAGAAGATGTAATTACATTTTTTTAAATGTATATATTTTTTAATGTAAATACTCAAAAACTATATTCTTAATAAAATTATTATTATTTTTTTTTCTGGGAAGGGGCATGCTCCTTTCCCA
AATCCTTAAAAGATAGCCAGCTATGGCTAGCTATCTTTGAGGAGTGTCTGCGTGTTTTTTGTTCTTAAT

Intron 4 GTGCAGAAAT ... TTGCAGCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	87.98	2.2	15	16
A2	88.2	3.4	26	24
A3	97.8	1.2	60	43
A4	85	2.4	13	11
A5	96.4	0.6	16	14
A6	76.59	19.2	63	58
A7	92.57	19.4	232	243
A8	93.64	13.8	211	193
A9	89.25	13.6	112	102
A10	94.62	14	246	232

>TRINITY_DN43335_c0_g4_i1 from sample A6 / Example spliced isoform

TTGGGAGGAGAAAAGATAGAGCGGAGCCGAGCAGAATTCAGGGAGCATATTTTAGTGTCTGCAGCAGGATGTCGCGGGGAATAGTGGGATTTCATGGTTCTGTGCGTGTCCTCATTGAAGCCGATGGTTCTGGGCGTGTCCTCATTGAAGCTGATGGTTCTGTGCGTGTCCTCCGTGAAGCCGATGGTTCTGGTAATACT
TGAAATTGCAACGCGTGGTGTAATTGATCAGAACAGATAGAGAGGAGCCCAGGAAAAGTTAGACAGATAGAGAGGAGCCGAGCAGATTTCAGGGTTTGTAAGAAGCAGAGATGAAGAAGAGCCACATCTGCTGGCTTGGCCAGTGCCAAACCCTGAAAAGATGAGAGAGTAGAGATGAAAATATATGAGCCAGAGCTACA
AGAGCAATAATCAGGTGCAGAAATCGTTGATGCTATCGTTGATGCTGCGGAATTTCGGGATTGCAGAGCAATAATCAGGACTGATTTCATGAACTAAAGAAGTCAAATTATGGATCCATACTTATTGTAGATGGATGACTTTTATGTTACTGCCATTGACAAGGACACTTCCATGTTCCATGGCATTTGTGGGAAACCTC
CTTGCAGAAGCAGTAAGGAATGCATACAGTCGACTCAAGAAGCGAGATTCAAATTGATTGTAAGCTAACAATATCCAAAGTATGCAGATATATAGGCAATCCCACTAGTGCTTGTGATTGATTGTTCACATAGCATCACTTGGAGGGCTTAGAATACTTTGGAGACAAGAACATAAAGAAATTATTGAAAATCTATGTAG
TAGATATATATTTGACAAAACCAAAAGATTACTAGAGAATTGAACAATAAATTCTTGGTGATAAACAAAATATAACAAAAGGTTGCATGTCCATAAACATAAATGCATTAACAAGGCATTTTGGGCATGTATCTTGTATGGGAATCGAATCTACGAACTTGACATAACAACAGAAAACAATAACATGTTTATTAATTCAA
AAGC

>TRINITY_DN43335_c0_g4_i17 from sample A6 / Example unspliced isoform:

TTTGGGTTTGCTTTATTCGTCTCTGTTTTGAGTTTGATTTGTTTGGGATCGAGAGGGAGGATGGAGGACTAAAAATATCCAGAAGTGGGTATCGCTGGGTTGTCATCCGCCAAGGCGGAGAAGAGCCAGAGCTACAAGAGCAATAATCAGgtgcagaaatcgttgatgctactcggggttgcagctagGTGCAGAAATCG
TTGATGCTGCTCGGGGTTGCAGGTGGGTTTTTAAGAAGAATAATCAGGTGCAGAAATCGTTGATGCTGCGGAATTTCGGGATTGCAGAGCAATAATCAGGTTGGCTTCGTTTAATTGACAATAGAAATGCTCTTTTTGGTTATTAAGCAGTGTTTTGAAGTTTTATAGGTGTTGTTTAGCAGAAGGGGATATAAATTTGA
GCTACCAGGATTTATATATTTATATATTTGTATACAGTTTTTATTTACCCTCATCAAAATCAAATTCCATGTGAGGGTTTGACTTTTTTTTTTTAAATCTTAGGTGACAGTGGTTTTAATGATAGAGATAGATAAGTG

Intron 5 GTTTGTGCTT ... TTTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.79	11	29	27
A2	30.56	25	12	10
A3	26.03	54	15	23
A4	50.94	13	9	18
A5	48.05	20	19	18
A6	24.84	59	18	21
A7	17.3	227	37	58
A8	20.3	210	54	53
A9	17.7	100	19	24
A10	13.91	229	30	44

>TRINITY_DN110095_c0_g3_i9 from sample A5 / Example spliced isoform

CTTATTTTATTTTACCTTCCATTCTGTGTTAATTTTTTTCTGTGTTGCATTTTTCTTTTTCTCTTTTTATTTTTATTTTTTATAATTTTTGCATTTTCCTTTTGGGTTTGCTTTATTCGTCTCTGTTTTGAGTTTGATTTGTTTGGGATCGAGAGGGAGGATGGAGGACTAAAAATATCAAGAAGTGGGTATCGCTGGGT
TGTCATCCGCCAAGGCGGAGAAGAGCCAGAGCTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTACTCGGGGTTGCAGCTAGGTGCAGAAATCGTTGATGCTGCTCGGGGTTGCAGGTGGGTTTTTAAGAAGAATAATCAGGTGCAGAAATCGTTGATGCCGCGGAATTTCGGGATTGCAGAGCAATAATCAGG
TGAGGTGGAGAAGAATGCAGGCTAGTTTGAAGTGCCTTCAGAGATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAAAGTGCAGGCTAGTTTGAAGTGCCTGCCTTTAGAGAAATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAGAGTGCAAGCTATTTTGAAGTGCCTCCACAAAAATATAAGGAAAAGGT
AAACAGTTTTTATTTTGATTAGATTTTAAATTTATCAAAAATATAAGGAAAAGGTAAACAGTTTTTATTTTGATTAGATTTTAAATTTATTGGTACCATTTTAAAAAATTAAATTTGTATAAATTAATCATATTTAGACTTTTTTATTATTTTTATAAATTTAAAATATTTTTGATGTGTACAGATGGGTGGATGAAGAG
TGCAAGTTATTTTTGCATTAATAGAATATTTGAAAGCGC

>TRINITY_DN110095_c0_g3_i5 from sample A5 / Example unspliced isoform:

CTTATTTTATTTTACCTTCCATTCTGTGTTAATTTTTTTCTGTGTTGCATTTTTCTTTTTCTCTTTTTATTTTTATTTTTTATAATTTTTGCATTTTCCTTTTGGGTTTGCTTTATTCGTCTCTGTTTTGAGTTTGATTTGTTTGGGATCGAGAGGGAGGATGGAGGACTAAAAATATCAAGAAGTGGGTATCGCTGGGT
TGTCATCCGCCAAGGCGGAGAAGgtttgtgcttaattaatgtgtaaatatttggattgaaatttaatctttattgcattatttcatcatgttctgttttgtgcagAGCCAGAGCTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTACTCGGGGTTGCAGCTAGGTGCAGAAATCGTTGATGCTGCTCGGGGTT
GCAGGTGGGTTTTTAAGAAGAATAATCAGGTGCAGAAATCGTTGATGCCGCGGAATTTCGGGATTGCAGAGCAATAATCAGGTGAGGTGGAGAAGAATGCAGGCTAGTTTGAAGTGCCTTCAGAGATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAAAGTGCAGGCTAGTTTGAAGTGCCTGCCTTTAGAGAAAT
ATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAGAGTGCAAGCTATTTTGAAGTGCCTCCACAAAAATATAAGGAAAAGGGATCACAACTCCTTGGCAGACAAACTTTCAAAGCACGCTATGGATGGTGAGGGCTTCTTGTTGTGGGAAGAATATCAGTGAACACACATCCAAGATATGGGTGTGTTAAGTTTTTTTGA
TTGAGATCAAGCTTTGAAATTTTAAATTTTACATTTCTCTTGATGTAATATTTTGGGGATTGGGATCATCTCTCTTATGTGTGATGTAATATTTTGGGATTGGGATTATCTCTCATATGTGTTTTTTGTGTTACTCCATTGAAAAAAAAATGAG

Intron 6 GTAAACAGTT ... ATGTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	30	14	16
A2	35.38	21	12	11
A3	36.36	28	19	13
A4	29.82	20	9	8
A5	51.72	7	6	9
A6	21.95	48	20	7
A7	14.99	295	72	32
A8	19.16	289	88	49
A9	16.76	144	39	19
A10	18.06	245	77	31

>TRINITY_DN124718_c5_g1_i8 from sample A2 / Example spliced isoform

TGGGTTTGCTTTATTCGTCTCTGTTTTGAGTTTGATTTGTTTGGGATCGAGAGGGAGGATGGAGGACTAAAAATATCCAGAAGTGGGTATCGCTGGGTTGTCATCCGCCAAGGCGGAGAAGAGCCAGAGCTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTACTCGGGGTTGCAGCTAGGTGCAGAAATCGTT
GATGCTGCTCGGGGTTGCAGGTGGGTTTTTAAGAAGAATAATCAGGTGCAGAAATCGTTGATGCCGCGGAATTTCGGGATTGCAGAGCAATAATCAGGTGAGGTGGAGAAGAATGCAGGCTAGTTTGAAGTGCCTTCAGAGAAATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAAAGTGCAGGCTAGTTTGAAGT
GCCTTTAGTGAAATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAGAGTGTGCAAGCTATTTTGAAGTGCCTCCACAAAAATATAAGGAAAAGGTGGGTGGATGAAGAGTGCAAGTTATTTTTGCATTGATAGAAAATTTGAAAGCGCTTTGCAGTAGACTAATAATAGAAGATGTAATTAGATAAGGAAAAGGTGG
GTGGATGAAGAGTGCAAGTTATTTTTGCATTGATAGAAAATTTGAAAGCGCTTTGCAGTAGA

>TRINITY_DN124718_c5_g1_i3 from sample A2 / Example unspliced isoform:

TGGGTTTGCTTTATTCGTCTCTGTTTTGAGTTTGATTTGTTTGGGATCGAGAGGGAGGATGGAGGACTAAAAATATCCAGAAGTGGGTATCGCTGGGTTGTCATCCGCCAAGGCGGAGAAGAGCCAGAGCTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTACTCGGGGTTGCAGCTAGGTGCAGAAATCGTT
GATGCTGCTCGGGGTTGCAGGTGGGTTTTTAAGAAGAATAATCAGGTGCAGAAATCGTTGATGCCGCGGAATTTCGGGATTGCAGAGCAATAATCAGGTGAGGTGGAGAAGAATGCAGGCTAGTTTGAAGTGCCTTCAGAGAAATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAAAGTGCAGGCTAGTTTGAAGT
GCCTTTAGTGAAATATAAGAAAAAGGTGTAACAGGTGAAGGTGGAGAAGAGTGTGCAAGCTATTTTGAAGTGCCTCCACAAAAATATAAGGAAAAGgtaaacagtttttattttgattagattttaaatttatcaaaaatataaggaaaaggtaaacagtttttattttgattagattttaaatttattggtaccatttt
aaaaaattaaatttgtataaattaatcatatttagacttttttattatttttataaatttaaaatatttttgatgtgtacagGTGGGTGGATGAAGAGTGCAAGTTATTTTTGCATTGATAGAAAATTTGAAAGCGCTTTGCAGTAGACTAATAATAGAAGATGTAATTACATTTTTTTAAATGTATATATTTTTTAATG
TAAATACTCAAAAACTATATTCTTAATAAAATTATTATTATTTTTTTTTCTGGGAAGGGGCATGCTCCTTTCCCAAATCCTTAAAAGATAGCCAGCTATGGCTAGCTATCTTTGAGGAGTGTCTGCGTGTTTTTTGTTCTTAAT

Intron 7 GTAATACTTG ... GGCGGAGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.46	16	72	8
A2	62.7	33	87	23
A3	82	15.5	88	53
A4	66.17	22.5	76	12
A5	79.22	6.5	33	16
A6	70.15	37.5	125	51
A7	77.88	52.5	160	209
A8	79.67	41	130	191
A9	73.62	39.5	130	90
A10	74.03	69.5	187	209

>TRINITY_DN47847_c1_g1_i3 from sample A10 / Example spliced isoform

GTGTAAGCAAATTTCAAACCCATGCTGGCATTGTTGGGAGAAGAAAAGATAGAGCGGAGCCGAGCAGAATTCAGGGAGCATATTTAAGTGTCTGCAGCAGGATGTCGCGGGGACTAGTGGGATTTCATGGTTCTGTGCGTGTCCTCATTGAAGCCGATGGTTCTGGGCGTGTCCTCATTGAAGCTGATGGTTCTGTGCGT
GTCCTCCGTGAAGCCGATGGTTCTGAGCCAGAGCTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTACTCGGGGTTGCAGCTAGGTGCAGAAATCGTTGATGCTGCTCGGGGTTGCA

>TRINITY_DN47847_c1_g1_i4 from sample A10 / Example unspliced isoform:

TTGGATTTCAAAGACGGCATGGGAAATTGTGCTGCAAGTGGATTTTAAAGACGGTTGTTGTAAGTAGATTTCAAATACCACTTTGGGAGACACGTGTAAGCATATTTCAAAACCCATCCATGAGAGGATTCCAAAGATGAGTAACTATAGAACTGAAATTTGAAATCACGAGGAGCGATATTTGGTAGCATTGCTGGGAG
AAGAAAAGGTTGACAGATAGAGAGGGCCGAGCGGATTTCAGGATTTGTAAGAAGCAGAGGTGATTTCAGGGTTTGTAAAAGGCGAGCATATTTCAGGGTTTGTGAGAAGCATAGGTGAGCATTGCTGGTGAGAAGAAAAGGTTGACAGATAGAGAGGAGCCGAGAAGATTTCAGGGAGCATATTTAAGTGTCTGCAGCAG
GATGTCGCGGGGAATAGTGGGATTTCATGGTTCTGTGCGTGTCCTCATTGAAGCCGATGGTTCTGGGCGTGTCCTCATTGAAGCTGATGGTTCTGTGCGTGTCCTCCGTGAAGCCGATGGTTCTGgtaatacttgaaattgcaacgcgtggtgtaattgatcagaacagatagagaggagcccaggaaaagttagacaga
tagagaggagccgagcagatttcagggtttgtaagaagcagagatgaagaagagccacatctgctggcttggccagtgccaaaccctgaaaagatgagagagtagagatcaaaatatatgatgagcgtccaataacttttgtgtttgattattgtgtttttctgagggcatggccatgcttattttattttaccttccat
tctgtgttaatttttttctgtgttgcatttttctttttctctttttatttttattttttataatttttgcattttccttttgggtttgctttattcgtctctgttttgagtttgatttgtttgggatcgagagggaggatggaggactaaaaatatccagaagtgggtatcgctgggttgtcatccgccaaggcggagaa
gAGCCAGAGCTACAAGAGCAATAATCAGGTGCAGAAATCGTTGATGCTACTCGGGGTTGCAGCTAGGTGCAGAAATCGTTGATGCTGCTCGGGGTTGCA

Intron 8 GCTAGTTTGA ... AAAAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.47	26	17	30
A2	41.38	17	7	17
A3	53.23	29	24	42
A4	41.77	23	7	26
A5	41.94	9	3	10
A6	40.3	80	41	67
A7	32.49	398	125	258
A8	32.1	350	100	231
A9	42.4	144	74	138
A10	39.52	303	140	256

>TRINITY_DN42826_c2_g1_i7 from sample A9 / Example spliced isoform

TTGGGTTTGCTTTGTTCGTCTCTGTTTTGAGTTTGATTTGTTTGGGATCGAGAGGGAGGATGGAGGACTAAAAATATCAAGAAGTGGGTATCGCTGGGTTGTCATCTGCGAAGGCGGAGAAGGTTTGTGCTTAATTAATCTTTATTGCATTATTTCATCATGTTCTGTTTTGTGCAGAGCCAGAGCTACAAGAGCAATAA
TCAGGTGCAGAAATCGTTGATGCTGCTCGGGGTTGCAGGTGGGTTTTTAAGAAGAATAATCAGGTGCAGAAATCGTTGATGCTGCGGAATTTCGGGATTGCAGAGCAATAATCAGGTGAGGTGGAGAAGAATGCAGGCTAGTTTGAAGTGCCTTCAGAGAAATATAAGAAAAAGGTGCAACAGGTGAAGGTGGAGAAGAG
TGCAAGCTATTTTGAAGTGCCTCCACAAAAATATAAGGAAAAG

>TRINITY_DN42826_c2_g1_i6 from sample A9 / Example unspliced isoform:

TGAGGTGGAGAAGAATGCAGgctagtttgaagtgccttcagagatataagaaaaaggtgtaacaggtgaaggtggagaaaagtgcagGCTAGTTTGAAGTGCCTTTAGAGAAATATAAGAAAAAGGTGCAACAGGTGAAGGTGGAGAAGAGTGTGCAAGCTATTTTGAAGTGCCTCCACAAAAATATAAGGAAAAGGTGG
GTGGATGAAGAGTGCAAGTTATTTTTGCATTGATAGAAAATTTGAAAGCGCTTTGCAGTAGACTAATAATAGAAGATGTAATTAGATAAGGAAAAGGTGGGTGGATGAAGAGTGCAAGTTATTTTTGCATTAATAGAATATTTGAAAGCGCTTTGCAGTAGACTAATAATAGAAGATGTAATTAGATAAGGAAAAGGTGG
GTGGATGA