sp|F4HT21|IAN9_ARATH ----> Immune-associated nucleotide-binding protein 9

Intron 1 GTGCTATATC ... GAAATTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.95	145.6	32.7	59
A2	24.74	240.1	62.9	95
A3	21.82	292	77	86
A4	25.82	173	61.4	59
A5	27.21	111	45	38
A6	14.73	343	33.5	85
A7	17.79	800.5	128.4	218
A8	18.89	489	77.7	150
A9	19.65	882.6	182.7	249
A10	18.97	803.2	171.1	205

>TRINITY_DN114236_c1_g2_i1 from sample A1 / Example spliced isoform

CCTCCATCATCCCTCCACCCCTCACTGTTCGGAGTCGGACTGGGCGTTAGCGACAAAATAGGCAGGGTTTGACGTTCTCATTATTCGTGGTGTCATCGGTTGCTGAAGCAGAATTCAGCAGCTCTTAGGTTATGCGACAAATACAGGGATTGGACTATAATTGAATTGGTGAACTCGAGCTCAATTTGCTATTTCAGGCG
ATTTAGTGAATTGGAAAAGGATAGGATGGGAGGAAGCGCAATGGAAGAGGTGGATGATTGGGAATTGACTGGACCTACCTCTGGGGAGACCACTCTGGTGTTGGTAGGGCGCACGGGCAATGGTAAAAGTGCAACTGGTAATTCCATCCTTGGTCGCAGGGCATTTACCTCACGTTCTCGTTCTGCTGCTGTTACACTCA
CTTCTGAATTGCAACAAGTCCAGATGAACGATGGCAGAAAATTGAATGTGGTCGATACGCCAGGTCTCTTTGATCCAACTGTCAATCCAGACTTTCTTGGCAAAGAAATTGTTAAATGTATCGACCTTGCAAAGGATGGTGTCCATGGTGTTCTTTTTGTACTTTCTGTTAGAAATCGATTTACTGCAGAAGAAGCAGCT
GCTTTAGAAAGCTTGCAAATGTTGTTTGGTGAGAAAATTCTCAATTACATGGTTGTGATCTTTACTGGTGGTGACGAGTTGGAAGAAAATCTGGAGACATTAGAAGACTACTTGCATGAAAGCCCACTTGAGCTTCAGACACTTCTACGCCAGTGCAATGATAGAAAGGTCCTTTTCAATAATAAAACCACATCAGAAAC
AATGAAGGCAAAGCAGACAGCTGAATTGCTCAAGCAAATTGATATTGTAATTGCTCAAAATGGAGGTCATCCTTATTCTAATGAGTTGTTTCGTGAAGCTCAGGAAAGGTTAAGTCGCCAAAAGGATATCGACTCGGGTGGATATTCAAAGGAAGAAATACAGTTTTTACAGAAACAGATGGAAAATGCATATGCTGAAC
AGCTTAAGCAATTAACTGAAATGGTAGAGGAGAAATTGCGTATAACTACAGAAAGGTTGGAACAAAGGTTGGCTTCTGAGCAGTCTGCAAGAGAACAGGCTGAAAAAAGAGCTCGAATTGAACAAGAAGACTCAGGTGAAAAAATCCGTATGCTTCAAGAAAAATTGCAAAAGGCTGAGGAGGAGACCGAAAATCTCAAG
AAGCAAATGGGCGGTGGCAACAAATGTGTGATTTTGTAATTGTGAATAATTTTGTTTCTAAAACTTATAGCAATATATATTAGCCAAAGGCTTACGATGTCTTGTAGGTATCAATACTGAGAATATGCAATTTAGTTTTGAATTATATGATCATGATTTACTACTTGTATTTCTCTCAACCTTTAATCTTAACCAGCTTT
GTGATACTAGACCTGAACAAGGGATGAGTGCTTCAATTTGGAATCTATGTATATAATTTACCTCTTTATGGTTTTTAAAAAGAGC

>TRINITY_DN114236_c1_g2_i2 from sample A1 / Example unspliced isoform:

CCTCCATCATCCCTCCACCCCTCACTGTTCGGAGTCGGACTGGGCGTTAGCGACAAAATAGGCAGGGTTTGACGTTCTCATTATTCGTGGTGTCATCGGTTGCTGAAGCAGAATTCAGCAGCTCTTAGgtgctatatccagttattcttgaaatatgtatttttaattctgcggcgagattagcccattcccaattttgg
attaatccaagttaagttttttgaaattggaggtttcttttgggcagccgaaagtttaaatcttgactatttctggaaattgaagGTTATGCGACAAATACAGGGATTGGACTATAATTGAATTGGTGAACTCGAGCTCAATTTGCTATTTCAGGCGATTTAGTGAATTGGAAAAGGATAGGATGGGAGGAAGCGCAATG
GAAGAGGTGGATGATTGGGAATTGACTGGACCTACCTCTGGGGAGACCACTCTGGTGTTGGTAGGGCGCACGGGCAATGGTAAAAGTGCAACTGGTAATTCCATCCTTGGTCGCAGGGCATTTACCTCACGTTCTCGTTCTGCTGCTGTTACACTCACTTCTGAATTGCAACAAGTCCAGATGAACGATGGCAGAAAATT
GAATGTGGTCGATACGCCAGGTCTCTTTGATCCAACTGTCAATCCAGACTTTCTTGGCAAAGAAATTGTTAAATGTATCGACCTTGCAAAGGATGGTGTCCATGGTGTTCTTTTTGTACTTTCTGTTAGAAATCGATTTACTGCAGAAGAAGCAGCTGCTTTAGAAAGCTTGCAAATGTTGTTTGGTGAGAAAATTCTCA
ATTACATGGTTGTGATCTTTACTGGTGGTGACGAGTTGGAAGAAAATCTGGAGACATTAGAAGACTACTTGCATGAAAGCCCACTTGAGCTTCAGACACTTCTACGCCAGTGCAATGATAGAAAGGTCCTTTTCAATAATAAAACCACATCAGAAACAATGAAGGCAAAGCAGACAGCTGAATTGCTCAAGCAAATTGAT
ATTGTAATTGCTCAAAATGGAGGTCATCCTTATTCTAATGAGTTGTTTCGTGAAGCTCAGGAAAGGTTAAGTCGCCAAAAGGATATCGACTCGGGTGGATATTCAAAGGAAGAAATACAGTTTTTACAGAAACAGATGGAAAATGCATATGCTGAACAGCTTAAGCAATTAACTGAAATGGTAGAGGAGAAATTGCGTAT
AACTACAGAAAGGTTGGAACAAAGGTTGGCTTCTGAGCAGTCTGCAAGAGAACAGGCTGAAAAAAGAGCTCGAATTGAACAAGAAGACTCAGGTGAAAAAATCCGTATGCTTCAAGAAAAATTGCAAAAGGCTGAGGAGGAGACCGAAAATCTCAAGAAGCAAATGGGCGGTGGCAACAAATGTGTGATTTTGTAATTGT
GAATAATTTTGTTTCTAAAACTTATAGCAATATATATTAGCCAAAGGCTTACGATGTCTTGTAGGTATCAATACTGAGAATATGCAATTTAGTTTTGAATTATATGATCATGATTTACTACTTGTATTTCTCTCAACCTTTAATCTTAACCAGCTTTGTGATACTAGACCTGAACAAGGGATGAGTGCTTCAATTTGGAA
TCTATGTATATAATTTACCTCTTTATGGTTTTTAAAAAGAGC

Intron 2 GTGCGCTCCT ... GCCAACCGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.06	9	8	8
A2	42.86	4	2	4
A3	47.06	9	7	9
A4	40.54	11	5	10
A5	42.86	4	2	4
A6	47.83	12	10	12
A7	46.07	24	19	22
A8	42.86	20	10	20
A9	46.03	17	13	16
A10	45.45	30	26	24

>TRINITY_DN38298_c0_g1_i2 from sample A6 / Example spliced isoform

GTCGACACGTTAACTTTTGAAGCCATTATATGACCTATCAAAAAGCTTCCTTCACCATATTCCTCGCTTCAATTCTTACTGAAGCAGAAGTCCCGCTTTTAATATCGAAATCGGAATCGAATTACGGGGAAGTTGCTAAACCTGGAATCGATTGACAGAAGTATAGGTTGATTTACAGGCGATTTATTGATTTGGAAGTC
CAAAATGATGGGAAGAAGCGAAGTAGACGAGGTGGATGATTGGGAATTGGCTGGACCTGCCACGGGGGAGACCACTGTGGTGTTGGTTGGGCGCACAGGCAATGGTAAAAGCGCAACTGGTAATTCCATTCTTGGGCGCAAGGCCTTCAAGTCTCGTTCCCGTTCTTCCGCTGTTACAGTCAGTTCTGAATTGCAACAAG
TCCAGATGAATGACGGCAGAATATTGAAGGTCGTAGATACACCAGGTCTCTTTGACCCTGCTGTCCATCCAGACTTTGTCGGCAAAGAAATTGTCAAATGTATTGACCTTGCAAAGCATGGCATCCATGGAGTTATTTTCGTACTTTCTGTTAGAAATAGATTTACTGCAGAAGAAGCAGCTGCTGTAGAGGGCTTGCAA
ATGTTGTTTGGGGACAAAATTCTCAACTACATGGTGGTTATCTTTACTGGCGGGGATGAGTTGGAAGAAAATCAAGAGACTTTAGAAGACTACTTGCATGACAGCCCACTTGAGCTTCAGGAGCTTCTCCGCCAGTGCGATAATAGAAAGGTCCTTTTCAATAACAAAGCTACTTCAAAAACAGTGCTGGCAAAACAAGT
AAATGAATTACTCAAGCAAACAGACATTATAATTGCTCAAAATGGCGGTCATCCTTATTCTAATGAGTTGTTCCGTGAAGCTCAGGAAAGGATGAGTCGACAAGAGGACATCGACTCAGGAGGATATTCGAAGGAAGAGATACAGCATTTGAAGATACAGATGGAAAATGCATACGCTGAACAGCTTAAAATATTAACTG
AAATGGTAGAGGAGAAATTGCGCATAACTACAGAAAAATTGGAGCAGCGATTGTCTTCTGAGCAGGCTGCACGAGAACAGGCTGAAAAAAGAGCTAAAAAAGAACAAGAAGAAGCAGGTGTGAAAATCAGTATGCTTCTAGAGAAATTGGAAAAGGCTCAGGAGGAGACTGAAAACCTCAAGAAGCAAATGGGCAGCGGC
AACAAATGTGTTATTTTGTAAGGTAACTGTTTATGTCTCCCAAACATATCAATTGAGTTACCGAAAGGCTCCAATGTTTTCTAGGTGTCTATATTGAGAATGTGTGTTTTTAGCTTTAATATATTTGATCATGTTTTACTGCATTAGTTTCTCTAACCTGTAATCTATATCAGATGTGTAAAATAGACCCAAGCAAGGGA
TGCCTGATTCAATTCGGTATCCAAGTATATATTGATTCCATTGGAATCCATGTATAGTTAAAAAGAGATACATATGATTAAAATATGGACACTAAGCAAGGGTTATCTCCAAGGATGCTAATCTTATAGTAATTTTGTTTTCTTTTTGGTAGTCCTATCATTTGTACTAGATTTAGAGAATCGTGAAGTTCTATGATACC
ATACATAAGAAGTTAGAACTATGTCAATCAATAATCTAGCTTAAAACATCTTTCCTTCAAGGAACCAGCTTGTCTATGGGCAATGAATTTATAGACTTAATTTATTCCTGTCAAACAGTCTAAGACTTTCAGAAATGCAGCGTTTCCGGAATTTTAAGTTTAGCAATTCCTATAATGTGGAGTCATAAATGTGTGATTAT
GTAATGACTGGGAGCATCATATTGAAATGATG

>TRINITY_DN38298_c0_g1_i4 from sample A6 / Example unspliced isoform:

GTCGACACGTTAACTTTTGAAGCCATTATATGACCTATCAAAAAGCTTCCTTCACCATATTCCTCGCTTCAATTCTTACTGAAGCAGAAGTCCCGCTTTTAATATCGAAATCGgtgcgctcctcagccaaccgagGAATCGAATTACGGGGAAGTTGCTAAACCTGGAATCGATTGACAGAAGTATAGGTTGATTTACAG
GCGATTTATTGATTTGGAAGTCCAAAATGATGGGAAGAAGCGAAGTAGACGAGGTGGATGATTGGGAATTGGCTGGACCTGCCACGGGGGAGACCACTGTGGTGTTGGTTGGGCGCACAGGCAATGGTAAAAGCGCAACTGGTAATTCCATTCTTGGGCGCAAGGCCTTCAAGTCTCGTTCCCGTTCTTCCGCTGTTACA
GTCAGTTCTGAATTGCAACAAGTCCAGATGAATGACGGCAGAATATTGAAGGTCGTAGATACACCAGGTCTCTTTGACCCTGCTGTCCATCCAGACTTTGTCGGCAAAGAAATTGTCAAATGTATTGACCTTGCAAAGCATGGCATCCATGGAGTTATTTTCGTACTTTCTGTTAGAAATAGATTTACTGCAGAAGAAGC
AGCTGCTGTAGAGGGCTTGCAAATGTTGTTTGGGGACAAAATTCTCAACTACATGGTGGTTATCTTTACTGGCGGGGATGAGTTGGAAGAAAATCAAGAGACTTTAGAAGACTACTTGCATGACAGCCCACTTGAGCTTCAGGAGCTTCTCCGCCAGTGCGATAATAGAAAGGTCCTTTTCAATAACAAAGCTACTTCAA
AAACAGTGCTGGCAAAACAAGTAAATGAATTACTCAAGCAAACAGACATTATAATTGCTCAAAATGGCGGTCATCCTTATTCTAATGAGTTGTTCCGTGAAGCTCAGGAAAGGATGAGTCGACAAGAGGACATCGACTCAGGAGGATATTCGAAGGAAGAGATACAGCATTTGAAGATACAGATGGAAAATGCATACGCT
GAACAGCTTAAAATATTAACTGAAATGGTAGAGGAGAAATTGCGCATAACTACAGAAAAATTGGAGCAGCGATTGTCTTCTGAGCAGGCTGCACGAGAACAGGCTGAAAAAAGAGCTAAAAAAGAACAAGAAGAAGCAGGTGTGAAAATCAGTATGCTTCTAGAGAAATTGGAAAAGGCTCAGGAGGAGACTGAAAACCT
CAAGAAGCAAATGGGCAGCGGCAACAAATGTGTTATTTTGTAAGGTAACTGTTTATGTCTCCCAAACATATCAATTGAGTTACCGAAAGGCTCCAATGTTTTCTAGGTGTCTATATTGAGAATGTGTGTTTTTAGCTTTAATATATTTGATCATGTTTTACTGCATTAGTTTCTCTAACCTGTAATCTATATCAGATGTG
TAAAATAGACCCAAGCAAGGGATGCCTGATTCAATTCGGTATCCAAGTATATATTGATTCCATTGGAATCCATGTATAGTTAAAAAGAGATACATATGATTAAAATATGGACACTAAGCAAGGGTTATCTCCAAGGATGCTAATCTTATAGTAATTTTGTTTTCTTTTTGGTAGTCCTATCATTTGTACTAGATTTAGAG
AATCGTGAAGTTCTATGATACCATACATAAGAAGTTAGAACTATGTCAATCAATAATCTAGCTTAAAACATCTTTCCTTCAAGGAACCAGCTTGTCTATGGGCAATGAATTTATAGACTTAATTTATTCCTGTCAAACAGTCTAAGACTTTCAGAAATGCAGCGTTTCCGGAATTTTAAGTTTAGCAATTCCTATAATGT
GGAGTCATAAATGTGTGATTATGTAATGACTGGGAGCATCATATTGAAATGATG

Intron 3 GTCTCTTTGA ... TGAGCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	63.64	86	135	166
A2	64.33	73	121.3	142
A3	72.61	54	142.3	144
A4	64.61	66	117	124
A5	62.42	31	53	50
A6	65.76	113	175	259
A7	67.83	236	380	615
A8	67.86	217	315.7	600.7
A9	67.77	249	424	623
A10	67.59	211	323	557

>TRINITY_DN103279_c0_g1_i3 from sample A5 / Example spliced isoform

CCTCACTGTTCGGAGTCGGACTGGGCGTTAGCGACAAAATAGGCAGGGTTTGACGTTCTCATTATTCGTGGTGTCATCGGTTGCTGAAGCAGAATTCAGCAGCTCTTAGGTTATGCGACAAATACAGGGATTGGACTATAATTGAATTGGTGAACTCGAGCTCAATTTGCTATTTCAGGCGATTTAGTGAATTGGAAAAG
GATAGGATGGGAGGAAGCGCAATGGAAGAGGTGGATGATTGGGAATTGACTGGACCTACCTCTGGGGAGACCACTCTGGTGTTGGTAGGGCGCACGGGCAATGGTAAAAGTGCAACTGGTAATTCCATCCTTGGTCGCAGGGCATTTACCTCACGTTCTCGTTCTGCTGCTGTTACACTCACTTCTGAATTACAACAAGT
CCAGATGAACGATGGCAGAAAATTGAATGTGGTCGATACGCCAGACACTTCTACGCCAGTGCAATGATAGAAAGGTCCTTTTCAATAATAAAACCACATCAGAAACAATGAAGGCAAAGCAGACAGCTGAATTGCTCAAGCAAATTGATATTGTAATTGCTCAAAATGGAGGTCATCCTTATTCTAATGAGTTGTTTCGT
GAAGCTCAGGAAAGGTTAAGTCGGCAAAAGGATATCGACTCGGGTGGATATTCAAAGGAAGAAATACAGTTTTTACAGAAACAGATGGAAAATGCATATGCTGAACAGCTTAAGCAATTAACTGAAATGGTAGAGGAGAAATTGCGTATAACTACAGAAAGGTTGGAACAAAGGTTGGCTTCTGAGCAGTCTGCAAGAGA
ACAGGCTGAAAAAAGAGCTCGAATTGAACAAGAAGAATCAGGTGAAAAAATCCGTATGCTTCAAGAGAAATTGCAAAAGGCTGAGGAGGAGACCGAAAATCTCAAGAAGCAAATGGGCGGTGGCAACAAATGTGTGATTTTGTAATTGTGAATAATTTTGTGTCTAAAACTTATAGCAATATATATTAGCCAAAGGCTTA
CGATGTCTTGTAGGTATCAATACTGAGAATATGCAATTTAGTTTTGAATTATATGATCATGATTTACTACTTGTATTTCTCTCAACCTTTAATCTTAACCAGCTTTGTGATACTAGACCTGAACAAGGGATGAGTGCTTCAATTTGGAATCTATGTATATAATTTACCTCTTTATGGTTTTTAAAAAGAGC

>TRINITY_DN103279_c0_g1_i1 from sample A5 / Example unspliced isoform:

CCTCACTGTTCGGAGTCGGACTGGGCGTTAGCGACAAAATAGGCAGGGTTTGACGTTCTCATTATTCGTGGTGTCATCGGTTGCTGAAGCAGAATTCAGCAGCTCTTAGGTTATGCGACAAATACAGGGATTGGACTATAATTGAATTGGTGAACTCGAGCTCAATTTGCTATTTCAGGCGATTTAGTGAATTGGAAAAG
GATAGGATGGGAGGAAGCGCAATGGAAGAGGTGGATGATTGGGAATTGACTGGACCTACCTCTGGGGAGACCACTCTGGTGTTGGTAGGGCGCACGGGCAATGGTAAAAGTGCAACTGGTAATTCCATCCTTGGTCGCAGGGCATTTACCTCACGTTCTCGTTCTGCTGCTGTTACACTCACTTCTGAATTGCAACAAGT
CCAGATGAACGATGGCAGAAAATTGAATGTGGTCGATACGCCAGgtctctttgatccaactgtcaatccagactttcttggcaaagaaattgttaaatgtatcgaccttgcaaaggatggtgtccatggtgttctttttgtactttctgttagaaatcgatttactgcagaagaagcagctgctttagaaagcttgcaaa
tgttgtttggtgagaaaattctcaattacatggttgtgatctttactggcggtgacgagttggaagaaaatctggagacattagaagactacttgcatgaaagcccacttgagcttcagACACTTCTACGCCAGTGCAATGATAGAAAGGTCCTTTTCAATAATAAAACCACATCAGAAACAATGAAGGCAAAGCAGACA
GCTGAATTGCTCAAGCAAATTGATATTGTAATTGCTCAAAATGGAGGTCATCCTTATTCTAATGAGTTGTTTCGTGAAGCTCAGGAAAGGTTAAGTCGGCAAAAGGATATCGACTCGGGTGGATATTCAAAGGAAGAAATACAGTTTTTACAGAAACAGATGGAAAATGCATATGCTGAACAGCTTAAGCAATTAACTGA
AATGGTAGAGGAGAAATTGCGTATAACTACAGAAAGGTTGGAACAAAGGTTGGCTTCTGAGCAGTCTGCAAGAGAACAGGCTGAAAAAAGAGCTCGAATTGAACAAGAAGAATCAGGTGAAAAAATCCGTATGCTTCAAGAGAAATTGCAAAAGGCTGAGGAGGAGACCGAAAATCTCAAGAAGCAAATGGGCGGTGGCA
ACAAATGTGTGATTTTGTAATTGTGAATAATTTTGTGTCTAAAACTTATAGCAATATATATTAGCCAAAGGCTTACGATGTCTTGTAGGTATCAATACTGAGAATATGCAATTTAGTTTTGAATTATATGATCATGATTTACTACTTGTATTTCTCTCAACCTTTAATCTTAACCAGCTTTGTGATACTAGACCTGAACA
AGGGATGAGTGCTTCAATTTGGAATCTATGTATATAATTTACCTCTTTATGGTTTTTAAAAAGAGC