sp|Q8VWK4|TRB1_ARATH ----> Telomere repeat-binding factor 1

Intron 1 GTAGTATCCT ... CTTTTGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.2	247	84	57
A2	19.38	258	85	39
A3	20	302	97	54
A4	21.18	173	62	31
A5	18.52	143	37	28
A6	22.54	330	118	74
A7	21.99	1112	398	229
A8	21.51	487	164	103
A9	21.45	976	352	181
A10	22.34	1022	378	210

>TRINITY_DN110196_c2_g3_i3 from sample A3 / Example spliced isoform

GGAAGACAGAATACTCAACCCACCATGTCAGATATAATTTAAACCCTGGAATATCAAAATTAATGTATTATTTGAAACAACATGGATATGCAGGTAGCGGAAGGGTTTGTATGGTTTGGTAGAGTTTGAATAGATGGGTGCACCGAAGCAGAAATGGACAAAGGAAGAGGAGGCGGCTCTTCGAGCGGGAATAGAAAAAT
ACGGCCCCGGTAAATGGTCTGCAATACTCAAGGATCCTGCCCTCAGTTGCTATCTGGCAACGCGTTCAAACGTCGATCTGAAGGATAAATGGCGAAATATTAATGTTACTGCCCATGGATGGGGGTCGAGGGAGAAAGCAAGATTGGCATTCAAAAAAAGCTCGCAGCTGGCAAAGCAAACTAACAAGCATCTCGCACTT
AGTACAATAACAAACGGCAGATCAAATATTGCAGTGGATGCTAGACCCTTGACAGCTGTAAATACATCTCTACCCAGCCCAGATACTCCCCGCTCTACTCCAAGGTTCGATCATATAGTTTTGGATGCTATCTCTAGTTTGAAGGAACCCAAAGGATCGAACAAATCTATCATAGCGATGTATATTGAGGATAATCACCT
GCCACCTCCAAACTTCAGAAAGTTGTTGTTGTCTCCCTCATTGAAGTCTCTAACAGAATGTGGTAAGCTGGTGAAGATGGGACAAAACTATATGATTGCTGGAAGTCTACTACAAACTGATGGAATGCCCAAAAGTGTACATTCACGACAGGAAGAGATGGAGGGAAAACGTTTTCCCATTCTTCTGAAGGACACTTCAA
GGAGATTGAGAGAAGAAATAAGGCTTATGACAAAATCTGAAATTGACGAAGAGCTTTCAGAGATGACATTGATGAATGCTGAAGAGGCTGCTAAAACTGCTGCTCAAATAGTGGCTGAAGCAGAAATAGCTATTGCTGAAGCAGAACAAGCGGCAAGGGAAGCAGAGGCAGCGGAAGCTGATGTAGAAGCTGCTCAGGCT
TTTGCAGAAGCAGCAATGATGACTCTAAGAAATTCTCGAAATTCTGTCAGAAGCTTGAGATGAGAAGTGCATTAGACAACAGTGTATCATTTGCAGGTCGTCGTCCAAGCAATGGAAAACTGACAATGATGAACTAGTGAAAGGAAATTAGAGGCAAATTTTTTGCTTTTGCTTTTGCATTGGTTACATCCCATTCAGAT
TTAGCCTTTTCCCATTCAGATTTAGCCTTTTATCTGCAATTTGACCAAAAGACACCGGGAGATTCATCATCATTTTTATCTTCTTGAGTTAGATGAAAGCTGTGAATAGTCCACCAATCTGAGTAGGAACTGCATCCATCACATGTATCACTCGGCATTGATGAAACTGAAGCATCTCATGATGAATCTGGGTTATCAAA
CCAGGACATTATAATTCAAAATGATTGTGTTCTGGACAGTACATGTATTACTTGATTGCTTTCAAGTTTCAGTCTTGTGGGATGCTATGATTCCAGCTGGATTTGATTTTCAAGAGAAAAAATCTTTGCTTCCTGGGATTGTACTTTCAAGAGAAATATATCATCGCATGCTAAGCTTAGCTGATTTTTCTTGTGTTTCA
GTGGGGACCGTGTAGTAGAGCTGCCTTTATCCATTTATGGATGCTGAATATTACAGGATTCTCCTGGACAAAATGTATTATCACTGACACACTTTATAGTCGATGTACCATCAAGAGTCTCATAGGTTTAAACGCTGGAATTTGTGCGGTATCTGTTCAAGCTTTGAATTATTATTCTCGAGTATGCTGGTGCTGTAGTT
TGATGAATGTGCATGTTGTAAACATTATTATGTGGTTTTAAGGTCTAAAGTTGCACAGG

>TRINITY_DN110196_c2_g3_i4 from sample A3 / Example unspliced isoform:

GGAAGACAGAATACTCAACCCACCATGTCAGATATAATTTAAACCCTGGAATATCAAAATTAATGTATTATTTGAAACAACATGGATATGCAGGTAGCGGAAGGGTTTGTATGGTTTGGTAGAGTTTGAATAGATGGGTGCACCGAAGCAGAAATGGACAAAGGAAGAGGAGGCGGCTCTTCGAGCGGGAATAGAAAAAT
ACGGCCCCGGTAAATGGTCTGCAATACTCAAGGATCCTGCCCTCAGTTGCTATCTGGCAACGCGTTCAAACGTCGATCTGAAGgtagtatcctgattctgtttttctctttttaaatatatttggttattttaatagttgtttggggtggatgatgggagagaattttgtataacggaccatcatgcttgtgactctttg
gtctgtctcctgtccaatagatgcctttggtgaagttgaggagtctacagtgcaggtctcaaatcctcaccataatggagtctcggtgatccatttttacaccattttctcaaattgcctttgatgagccggtggtctgtctgttctcaagctcaatactgccaatagaatagggttgtttcctactctattgcaccatc
attcgcatcgagggggtggttcccctatcgataactggattggtgaaagcaaggtagtgtcatagctctcgcaatgttcaattcctaaaagaagtgacaggaacacttacaagggggctgttgattagtggggctgccatggctgttaaatcaagccgtgtggtacatgtcccagaaatacatggaagcgccgacctttt
gaaagGATAAATGGCGAAATATTAATGTTACTGCCCATGGATGGGGGTCGAGGGAGAAAGCAAGATTGGCATTCAAAAAAAGCTCGCAGCTGGCAAAGCAAACTAACAAGCATCTCGCACTTAGTACAATAACAAACGGCAGATCAAATATTGCAGTGGATGCTAGACCCTTGACAGCTGTAAATACATCTCTACCCAGC
CCAGATACTCCCCGCTCTACTCCAAGGTTCGATCATATAGTTTTGGATGCTATCTCTAGTTTGAAGGAACCCAAAGGATCGAACAAATCTATCATAGCGATGTATATTGAGGATAATCACCTGCCACCTCCAAACTTCAGAAAGTTGTTGTTGTCTCCCTCATTGAAGTCTCTAACAGAATGTGGTAAGCTGGTGAAGAT
GGGACAAAACTATATGATTGCTGGAAGTCTACTACAAACTGATGGAATGCCCAAAAGTGTACATTCACGACAGGAAGAGATGGAGGGAAAACGTTTTCCCATTCTTCTGAAGGACACTTCAAGGAGATTGAGAGAAGAAATAAGGCTTATGACAAAATCTGAAATTGACGAAGAGCTTTCAGAGATGACATTGATGAATG
CTGAAGAGGCTGCTAAAACTGCTGCTCAAATAGTGGCTGAAGCAGAAATAGCTATTGCTGAAGCAGAACAAGCGGCAAGGGAAGCAGAGGCAGCGGAAGCTGATGTAGAAGCTGCTCAGGCTTTTGCAGAAGCAGCAATGATGACTCTAAGAAATTCTCGAAATTCTGTCAGAAGCTTGAGATGAGAAGTGCATTAGACA
ACAGTGTATCATTTGCAGGTCGTCGTCCAAGCAATGGAAAACTGACAATGATGAACTAGTGAAAGGAAATTAGAGGCAAATTTTTTGCTTTTGCTTTTGCATTGGTTACATCCCATTCAGATTTAGCCTTTTCCCATTCAGATTTAGCCTTTTATCTGCAATTTGACCAAAAGACACCGGGAGATTCATCATCATTTTTA
TCTTCTTGAGTTAGATGAAAGCTGTGAATAGTCCACCAATCTGAGTAGGAACTGCATCCATCACATGTATCACTCGGCATTGATGAAACTGAAGCATCTCATGATGAATCTGGGTTATCAAACCAGGACATTATAATTCAAAATGATTGTGTTCTGGACAGTACATGTATTACTTGATTGCTTTCAAGTTTCAGTCTTGT
GGGATGCTATGATTCCAGCTGGATTTGATTTTCAAGAGAAAAAATCTTTGCTTCCTGGGATTGTACTTTCAAGAGAAATATATCATCGCATGCTAAGCTTAGCTGATTTTTCTTGTGTTTCAGTGGGGACCGTGTAGTAGAGCTGCCTTTATCCATTTATGGATGCTGAATATTACAGGATTCTCCTGGACAAAATGTAT
TATCACTGACACACTTTATAGTCGATGTACCATCAAGAGTCTCATAGGTTTAAACGCTGGAATTTGTGCGGTATCTGTTCAAGCTTTGAATTATTATTCTCGAGTATGCTGGTGCTGTAGTTTGATGAATGTGCATGTTGTAAACATTATTATGTGGTTTTAAGGTCTAAAGTTGCACAGG

Intron 2 GTAGTATCCT ... CTACTCCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	83	84	82
A2	48.37	87	85	78
A3	49.51	104	97	107
A4	50.2	62	62	63
A5	51.11	44	37	55
A6	48.44	132	118	130
A7	44.6	464	398	349
A8	50.6	186	164	217
A9	47.65	396	352	369
A10	44.9	440	378	339

>TRINITY_DN100785_c0_g2_i3 from sample A5 / Example spliced isoform

TGGAGTACGCGGTTTACAATCTATGCGAGCACAAGCCAGTGGATGGGTTTTTCCTGGCCTTGATCAATACATCCGTACTTGAAAACAGTCAGCGGAAGGTAGCGGAAGGGTTTGTATGGTTTGGTAGAGTTTGAATAGATGGGTGCACCGAAGCAGAAATGGACAAAGGAAGAGGAGGCGGCTCTTCGAGCGGGAATAGA
AAAATACGGCCCCGGTAAATGGTCTGCAATACTCAAGGATCCTGCCCTCAGTTGCTATCTGGCAACGCGTTCAAACGTCGATCTGAAGGTTCGATCATATAGTTTTGGATGCTATCTCTAGTTTGAAGGAACCCAAAGGATCGAACAAATCTATCATAGCGATGTATATTGAGGTAGCTTTTTTGTGAATTAGCATTCTG
ACAGTTACACAAATTTAGGGTTGTTATTCAAAGCTAAAATCCAAGTTAATAAGCACAAACATAAAAACAATTATTTGAACATTAAATATGCTTTGAAGTATCC

>TRINITY_DN100785_c0_g2_i7 from sample A5 / Example unspliced isoform:

ATTTTTTGGAAGACAGAATACTCAACCCACCATGTCAGATATAATTTAAACCCTGGAATATCAAAATTAATGTATTATTTGAAACAACATGGATATGCAGGTAGCGGAAGGGTTTGTATGGTTTGGTAGAGTTTGAATAGATGGGTGCACCGAAGCAGAAATGGACAAAGGAAGAGGAGGCGGCTCTTCGAGCGGGAATA
GAAAAATACGGCCCCGGTAAATGGTCTGCAATACTCAAGGATCCTGCCCTCAGTTGCTATCTGGCAACGCGTTCAAACGTCGATCTGAAGgtagtatcctgattctgtttttctctttttaaatatatttggttattttaatagttgtttggggtggatgatgggagagaattttgtataacggaccatcatgcttgtga
ctctttggtctgtctcctgtccaatagatgcctttggtgaagttgaggagtctacagtgcaggtctcaaatcctcaccataatggagtctcggtgatccatttttacaccattttctcaaattgcctttgatgagccggtggtctgtctgttctcaagctcaatactgccaatagaatagggttgtttcctactctattg
caccatcattcgcatcgagggggtggttcccctatcgataactggattggtgaaagcaaggtagtgtcatagctctcgcaatgttcaattcctaaaagaagtgacaggaacacttacaagggggctgttgattagtggggctgccatggctgttaaatcaagccgtgtggtacatgtcccagaaatacatggaagcgccg
accttttgaaaggataaatggcgaaatattaatgttactgcccatggatgggggtcgagggagaaagcaagattggcattcaaaaaaagctcgcagctggcaaagcaaactaacaagcatctcgcacttagtacaataacaaacggcagatcaaatattgcagtggatgctagacccttgacagctgtaaatacatctct
acccagcccagatactccccgctctactccaagGTTCGATCATATAGTTTTGGATGCTATCTCTAGTTTGAAGGAACCCAAAGGATCGAACAAATCTATCATAGCGATGTATATTGAGG

Intron 3 GTTAGTAATA ... TTATCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40.89	144	115	84.2
A2	41.7	79	80	33
A3	39.39	100	79	51
A4	42.49	111	109	55
A5	45.95	30	31	20
A6	40.43	226	156	150.8
A7	40.2	407	284	263.2
A8	45.38	389	351	295.2
A9	37.45	421	253	251.2
A10	45.92	232	238	156

>TRINITY_DN114891_c3_g2_i2 from sample A1 / Example spliced isoform

CGATTTTTTGGAAGACAGAATACTCAACCCACCATGTCAGATATAATTTAAACCCTGGAATATCAAAATTAATGTATTATTTGAAACAACATGGATATGCAGGTAGCGGAAGGGTTTGTATGGTTTGGTAGAGTTTGAATAGATGGGTGCACCGAAGCAGAAATGGACAAAGGAAGAGGAGGCGGCTCTTCGAGCGGGAA
TAGAAAAATACGGCCCCGGTAAATGGTCTGCAATACTCAAGGATCCTGCCCTCAGTTGCTATCTGGCAACGCGTTCAAACGTCGATCTGAAGGATAAATGGCGAAATATTAATGTTACTGCCCATGGATGGGGGTCGAGGGAGAAAGCAAGATTGGCATTCAAAAAAAGCTCGCAGCTGGCAAAGCAAACTAACAAGCAT
CTCGCACTTAGTACAATAACAAACGGCAGATCAAATATTGCAGTGGATGCTAGACCCTTGACAGCTGTAAATACATCTCTACCCAGCCCAGATACTCCCCGCTCTACTCCAAGGTTCGATCATATAGTTTTGGATGCTATCTCTAGTTTGAAGGAACCCAAAGGATCGAACAAATCTATCATAGCGATGTATATTGAGGA
TAATCACCTGCCACCTCCAAACTTCAGAAAGTTGTTGTTGTCTCCCTCATTGAAGTCTCTAACAGAATGTGGTAAGCTGGTGAAGATGGGACAAAACTATATGATTGCTGGAAGTCTACTACAAACTGATGGAATGCCCAAAAGTGTACATTCACGACAGGAAGAGATGGAGGGAAAACGTTTTCCCATTCTTCTGAAGG
ACACTTCAAGGAGATTGAGAGAAGAAATAAGGCTTATGACAAAATCTGAAATTGACGAAGAGCTTTCAGAGATGACATTGATGAATGCTGAAGAGGCTGCTAAAACTGCTGCTCAAATAGTGGCTGAAGCAGAAATAGCTATTGCTGAAGCAGAACAAGCGGCAAGGGAAGCAGAGGCAGCGGAAGCTGATGTAGAAGCT
GCTCAGGCTTTTGCAGAAGCAGCAATGATGACTCTAAGAAATTCTCGAAATTCTGTCAGAAGCTTGAGATGAGAAGTGCATTAGACAACAGTGTATCATTTGCAGGTCGTCGTCCAAGCAATGGAAAACTGACAATGATGAACTAGTGAAAGGAAATTAGAGGCAAATTTTTTGCTTTTGCTTTTGCATTGGTTACATCC
CATTCAGATTTAGCCTTTTCCCATTCAGATTTAGCCTTTTATCTGCAATTTGACCAAAAGACACCGGGAGATTCATCATCATTTTTATCTTCTTGAGTTAGATGAAAGCTGTGAATAGTCCACCAATCTGAGTAGGAACTGCATCCATCACATGTATCACTCGGCATTGATGAAACTGAAGCATCTCATGATGAATCTGG
GTTATCAAACCAGGACATTATAATTCAAAATGATTGTGTTCTGGACAGTACATGTATTACTTGATTGCTTTCAAGTTTCAGTCTTGTGGGATGCTATGATTCCAGCTGGATTTGATTTTCAAGAGAAAAAATCTTTGCTTCCTGGGATTGTACTTTCAAGAGAAATATATCATCGCATGCTAAGCTTAGCTGATTTTTCT
TGTGTTTCAGTGGGGACCGTGTAGTAGAGCTGCCTTTATCCATTTATGGATGCTGAATATTACAGGATTCTCCTGGACAAAATGTATTATCACTGACACACTTTATAGTCGATGTACCATCAAGAGTCTCATAGGTTTAAACGCTGGAATTTGTGCGGTATCTGTTCAAGCTTTGAATTATTATTCTCGAGTATGCTGGT
GCTGTAGTTTGATGAATGTGCATGTTGTAAACATTATTATGTGGTTTTAAGGTCTAAAGTTGCACAGGTCTTAGTTTGGGTTTTTTGTCAATTTTTAAAT

>TRINITY_DN114891_c3_g2_i5 from sample A1 / Example unspliced isoform:

CGATTTTTTGGAAGACAGAATACTCAACCCACCATGTCAGATATAATTTAAACCCTGGAATATCAAAATTAATGTATTATTTGAAACAACATGGATATGCAGGTAGCGGAAGGGTTTGTATGGTTTGGTAGAGTTTGAATAGATGGGTGCACCGAAGCAGAAATGGACAAAGGAAGAGGAGGCGGCTCTTCGAGCGGGAA
TAGAAAAATACGGCCCCGGTAAATGGTCTGCAATACTCAAGGATCCTGCCCTCAGTTGCTATCTGGCAACGCGTTCAAACGTCGATCTGAAGGATAAATGGCGAAATATTAATGTTACTGCCCATGGATGGGGGTCGAGGGAGAAAGCAAGATTGGCATTCAAAAAAAGCTCGCAGCTGGCAAAGCAAACTAACAAGCAT
CTCGCACTTAGTACAATAACAAACGGCAGATCAAATATTGCAGTGGATGCTAGACCCTTGACAGCTGTAAATACATCTCTACCCAGCCCAGATACTCCCCGCTCTACTCCAAGGTTCGATCATATAGTTTTGGATGCTATCTCTAGTTTGAAGGAACCCAAAGGATCGAACAAATCTATCATAGCGATGTATATTGAGGA
TAATCACCTGCCACCTCCAAACTTCAGAAAGTTGTTGTTGTCTCCCTCATTGAAGTCTCTAACAGAATGTGGTAAGCTGGTGAAGATGGGACAAAACTATATGATTGCTGGAAGTCTACTACAAACTGATGGAATGCCCAAAAGTGTACATTCACGACAGGAAGAGATGGAGGGAAAACGTTTTCCCATTCTTCTGAAGG
ACACTTCAAGGAGATTGAGAGAAGAAATAAGGCTTATGACAAAATCTGAAATTGACGAAGAGCTTTCAGAGATGACATTGATGAATGCTGAAGAGGCTGCTAAAACTGCTGCTCAAATAGTGGCTGAAGCAGAAATAGCTATTGCTGAAGCAGAACAAGCGGCAAGGGAAGCAGAGGCAGCGGAAGCTGATGTAGAAGCT
GCTCAGGCTTTTGCAGAAGCAGCAATGATGACTCTAAGAAATTCTCGAAATTgttagtaatagtctctgtatacggtatcctttgatagagccaagtggtagtaatggctttagcttgtgagtaaactggctgtattgattctgtttgttttgacatatcatgtactaactggaaagtctgtatattgttttatctacag


CTGTCAGAAGCTTGAGATGAGAAGTGCATTAGACAACAGTGTATCATTTGCAGGTCGTCGTCCAAGCAATGGAAAACTGACAATGATGAACTAGTGAAAGGAAATTAGAGGCAAATTTTTTGCTTTTGCTTTTGCATTGGTTACATCCCATTCAGATTTAGCCTTTTCCCATTCAGATTTAGCCTTTTATCTGCAATTTG
ACCAAAAGACACCGGGAGATTCATCATCATTTTTATCTTCTTGAGTTAGATGAAAGCTGTGAATAGTCCACCAATCTGAGTAGGAACTGCATCCATCACATGTATCACTCGGCATTGATGAAACTGAAGCATCTCATGATGAATCTGGGTTATCAAACCAGGACATTATAATTCAAAATGATTGTGTTCTGGACAGTACA
TGTATTACTTGATTGCTTTCAAGTTTCAGTCTTGTGGGATGCTATGATTCCAGCTGGATTTGATTTTCAAGAGAAAAAATCTTTGCTTCCTGGGATTGTACTTTCAAGAGAAATATATCATCGCATGCTAAGCTTAGCTGATTTTTCTTGTGTTTCAGTGGGGACCGTGTAGTAGAGCTGCCTTTATCCATTTATGGATG
CTGAATATTACAGGATTCTCCTGGACAAAATGTATTATCACTGACACACTTTATAGTCGATGTACCATCAAGAGTCTCATAGGTTTAAACGCTGGAATTTGTGCGGTATCTGTTCAAGCTTTGAATTATTATTCTCGAGTATGCTGGTGCTGTAGTTTGATGAATGTGCATGTTGTAAACATTATTATGTGGTTTTAAGG
TCTAAAGTTGCACAGGTCTTAGTTTGGGTTTTTTGTCAATTTTTAAAT