sp|Q9FG08|ATXR4_ARATH ----> Histone-lysine N-methyltransferase ATXR4

Intron 1 GTAAGGTCTG ... GCAGGCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	1	3
A2	100	0	2	8
A3	100	0	2	8
A4	63.64	2	3	4
A5	100	0	2	0
A6	76.19	5	8	24
A7	75.76	8	25	25
A8	80.49	4	14	19
A9	76.47	8	32	20
A10	86.96	3	25	15

>TRINITY_DN39981_c0_g1_i3 from sample A6 / Example spliced isoform

GTGCACATAAAATGTTTGGTCCTGGGAATATGAATTTGAATCATTTGGCACTCGTTTACTTCCGCATTATTCCTTCTCAAAATTTCATACATCTACTGTTATTATTTGCTTTTTCATGAAGAAAAACTGACCGCCGAGCATGAAGATGATGAAGAGCAGCAGGAGGTTTTGGGTTTGGCTTCCGCGATTATCATTGTCTC
GTTATCAATCCAACAACGTTTTTTCTTCTTGTTCTTTCCATCCAGAGGCGCCACCACCTCCCGTTCGAGTGGGTTTCACAGAAAACGCGGGCAGAGGAGTTTTCGCTACTCGGGAGATTCGAACCGGCGACCTGATACACACCGCTGATCCCTTTGTGGCTCACCCTTCCCTGGAAGCTTTGGGGCTTTTACGAAATCGA
GATGAAAATGGATTGGTCGTTATATCATGAGCATTGCAGAGTGAAAGGCTTGAAGTACCCATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACCAGCAAATTTTTCTCATGGAATGTCTTCAGCGTGGGAAGATGAGTTTGAGTTATTGGGACACAATT
TCAAGAACGCTGACATTCGTCCTGACCTGTTGACTTTTCTTACAAAAGAATGGTACTCTGGTATTCTGGCTCGCATCCGTGCAAATGTTTTCAGGATTGAATTGATTGGAGGATACCATACCGATCTCCTTGCAGCTGCATCGGCATCTATTTCTGTTGAAGCAACTGTGGGTAGTGCTGTTTACATGCTGCCCTCAATG
TACAATCACGATTGTGATCCAAATGCACACATATTGTGGATAGAAAATGCAAAAGCCAGGTTGAAGGCTCTTCGTAATATTGAATCAGGTGAAGAACTACGTATTTGCTACATCGATGCTAGTATGGATTATGAAGCTCGACAATCTCTACTTTATCAAGGCTTTGGCTTTCGGTGTCAATGCCTTCGATGCAAATCAAA
GGACTAGATCTTATATAAAGGATGACGAACTTTTATTAAATGGATTCCATCATAGTGAGATATATTCAGAGCTAAAGTTACCCCTTGCATGTTACGAATCATATGACATCTCAAAAGCTTACATACGCAACATTGAAGTATTGTTTACTTTCTTAATCATACTCTAAAGTGGGGACTAGAATTTGTGACTACTTACAATT
ATAAAAATAATATAGCAAGAATTATCAAATAAGAGATAGAGAGGTTGTTTATATACTTGATTATGTTTTCTAATTAGAATTGATGTTCTCTTCAAAAACTCATGAATTTCCTCGGAGCCATGGAAAGCATACTTCATCGAGATAATAATGTGATATTTAATTATGC

>TRINITY_DN39981_c0_g1_i2 from sample A6 / Example unspliced isoform:

GTGCACATAAAATGTTTGGTCCTGGGAATATGAATTTGAATCATTTGGCACTCGTTTACTTCCGCATTATTCCTTCTCAAAATTTCATACATCTACTGTTATTATTTGCTTTTTCATGAAGAAAAACTGACCGCCGAGCATGAAGATGATGAAGAGCAGCAGGAGGTTTTGGGTTTGGCTTCCGCGATTATCATTGTCTC
GTTATCAATCCAACAACGTTTTTTCTTCTTGTTCTTTCCATCCAGGTCTTTTTCTGTAATGCCCTTCAACGCGTCAGATTCCTTTACATTTCAAGGTATTTTAATGAACTAAAAGTATTAGTAGCAATATTGTGCAATACCAGAGGCGCCACCACCTCCCGTTCGAGTGGGTTTCACAGAAAACGCGGGCAGAGGAGTTT
TCGCTACTCGGGAGATTCGAACCGGCGACCTGATACACACCGCTGATCCCTTTGTGGCTCACCCTTCCCTGGAAGCTTTGGgtaaggtctgctattcatgtttggggagattgaaagaagtaaaatgccatttgccaactttttcttcaagttcacaatgggagaatgcaagagcatctgcagatgctttacgtgatggt
caaaacgacatttctttttgcagtagtgaatgcacagagcaggcaaagGGCTTTTACGAAATCGAGATGAAAATGGATTGGTCGTTATATCATGAGCATTGCAGAGTGAAAGGCTTGAAGTACCCATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACC
AGCAAATTTTTCTCATGGAATGTCTTCAGCGTGGGAAGATGAGTTTGAGTTATTGGGACACAATTTCAAGAACGCTGACATTCGTCCTGACCTGTTGACTTTTCTTACAAAAGAATGGTACTCTGGTATTCTGGCTCGCATCCGTGCAAATGTTTTCAGGATTGAATTGATTGGAGGATACCATACCGATCTCCTTGCAG
CTGCATCGGCATCTATTTCTGTTGAAGCAACTGTGGGTAGTGCTGTTTACATGCTGCCCTCAATGTACAATCACGATTGTGATCCAAATGCACACATATTGTGGATAGAAAATGCAAAAGCCAGGTTGAAGGCTCTTCGTAATATTGAATCAGGTGAAGAACTACGTATTTGCTACATCGATGCTAGTATGGATTATGAA
GCTCGACAATCTCTACTTTATCAAGGCTTTGGCTTTCGGTGTCAATGCCTTCGATGCAAATCAAAGGACTAGATCTTATATAAAGGATGACGAACTTTTATTAAATGGATTCCATCATAGTGAGATATATTCAGAGCTAAAGTTACCCCTTGCATGTTACGAATCATATGACATCTCAAAAGCTTACATACGCAACATTG
AAGTATTGTTTACTTTCTTAATCATACTCTAAAGTGGGGACTAGAATTTGTGACTACTTACAATTATAAAAATAATATAGCAAGAATTATCAAATAAGAGATAGAGAGGTTGTTTATATACTTGATTATGTTTTCTAATTAGAATTGATGTTCTCTTCAAAAACTCATGAATTTCCTCGGAGCCATGGAAAGCATACTTC
ATCGAGATAATAATGTGATATTTAATTATGC

Intron 2 GTATCCTTCA ... ATGGTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.73	10	1.5	2
A2	0	4	0	0
A3	20.2	7.9	4.8	2
A4	50	4	0	4
A5	0	1	0.1	0
A6	26.72	32.9	3.5	12
A7	11.14	31.9	1.2	4
A8	36.71	25.9	2.5	15
A9	39.06	25	6.5	16
A10	27.96	25.8	2.6	10

>TRINITY_DN39981_c0_g1_i4 from sample A6 / Example spliced isoform

AGGCGCCACCACCTCCCGTTCGAGTGGGTTTCACAGAAAACGCGGGCAGAGGAGTTTTCGCTACTCGGGAGATTCGAACCGGCGACCTGATACACACCGCTGATCCCTTTGTGGCTCACCCTTCCCTGGAAGCTTTGGGTCTGCTATTCATGTTTGGGGAGATTGAAAGAAGTAAAATGCCATTTGCCAACTTTTTCTTC
AAGTTCACAATGGGAGAATGCAAGAGCATCTGCAGATGCTTTACGTGATGGTCAAAACGACATTTCTTTTTGCAGTAGTGAATGCACAGAGCAGGCAAAGAGTGAAAGGCTTGAAGTACCCATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACCAGCA
AATTTTTCTCATGGAATGTCTTCAGCGTGGGAAGATGAGTTTGAGTTATTGGGACACAATTTCAAGAACGCTGACATTCGTCCTGACCTGTTGACTTTTCTTACAAAAGAATGGTACTCTGGTATTCTGGCTCGCATCCGTGCAAATGTTTTCAGGATTGAATTGATTGGAGGATACCATACCGATCTCCTTGCAGCTGC
ATCGGCATCTATTTCTGTTGAAGCAACTGTGGGTAGTGCTGTTTACATGCTGCCCTCAATGTACAATCACGATTGTGATCCAAATGCACACATATTGTGGATAGAAAATGCAAAAGCCAGGTTGAAGGCTCTTCGTAATATTGAATCAGGTGAAGAACTACGTATTTGCTACATCGATGCTAGTATGGATTATGAAGCTC
GACAATCTCTACTTTATCAAGGCTTTGGCTTTCGGTGTCAATGCCTTCGATGCAAATCAAAGGACTAGATCTTATATAAAGGATGACGAACTTTTATTAAATGGATTCCATCATAGTGAGATATATTCAGAGCTAAAGTTACCCCTTGCATGTTACGAATCATATGACATCTCAAAAGCTTACATACGCAACATTGAAGT
ATTGTTTACTTTCTTAATCATACTCTAAAGTGGGGACTAGAATTTGTGACTACTTACAATTATAAAAATAATATAGCAAGAATTATCAAATAAGAGATAGAGAGGTTGTTTATATACTTGATTATGTTTTCTAATTAGAATTGATGTTCTCTTCAAAAACTCATGAATTTCCTCGGAGCCATGGAAAGCATACTTCATCG
AGATAATAATGTGATATTTAATTATGC

>TRINITY_DN39981_c0_g1_i6 from sample A6 / Example unspliced isoform:

GTGCACATAAAATGTTTGGTCCTGGGAATATGAATTTGAATCATTTGGCACTCGTTTACTTCCGCATTATTCCTTCTCAAAATTTCATACATCTACTGTTATTATTTGCTTTTTCATGAAGAAAAACTGACCGCCGAGCATGAAGATGATGAAGAGCAGCAGGAGGTTTTGGGTTTGGCTTCCGCGATTATCATTGTCTC
GTTATCAATCCAACAACGTTTTTTCTTCTTGTTCTTTCCATCCAGAGGCGCCACCACCTCCCGTTCGAGTGGGTTTCACAGAAAACGCGGGCAGAGGAGTTTTCGCTACTCGGGAGATTCGAACCGGCGACCTGATACACACCGCTGATCCCTTTGTGGCTCACCCTTCCCTGGAAGCTTTGGGTCTGCTATTCATGTTT
GGGGAGATTGAAAGAAGTAAAATGCCATTTGCCAACTTTTTCTTCAAGTTCACAATGGGAGAATGCAAGAGCATCTGCAGATGCTTTACGTGATGGTCAAAACGACATTTCTTTTTGCAGTAGTGAATGCACAGAGCAGGCAAAGGGCTTTTACGAAATCGAGATGAAAATGGATTGGTCGTTATATCATGAGCATTGCA
GAGTGAAAGGCTTGAAGTACCCATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACCAGCAAATTTTTCTCATGGAATGTCTTCAGCGTTTACAGTGGGAAGATGAGTTTGAGTTATTGGGACACAATTTCAAGAACGCTGACATTCGTCCTGACCTGTT
GACTTgtatccttcaaaatattttgaattatcatgccaaagtgctagtctgtatgaggggatgtatatctgagtggactatctccaaggaacttttttcttcagcgcttatgtggctttgttgtgctttgacatggtgtcagTTCTTACAAAAGAATGGTACTCTGGTATTCTGGCTCGCATCCGTGCAAATGTTTTCAG
GATTGAATTGATTGGAGGATACCATACCGATCTCCTTGCAGCTGCATCGGCATCTATTTCTGTTGAAGCAACTGTGGGTAGTGCTGTTTACATGCTGCCCTCAATGTACAATCACGATTGTGATCCAAATGCACACATATTGTGGATAGAAAATGCAAAAGCCAGGTTGAAGGCTCTTCGTAATATTGAATCAGGTGAAG
AACTACGTATTTGCTACATCGATGCTAGTATGGATTATGAAGCTCGACAATCTCTACTTTATCAAGGCTTTGGCTTTCGGTGTCAATGCCTTCGATGCAAATCAAAGGACTAGATCTTATATAAAGGATGACGAACTTTTATTAAATGGATTCCATCATAGTGAGATATATTCAGAGCTAAAGTTACCCCTTGCATGTTA
CGAATCATATGACATCTCAAAAGCTTACATACGCAACATTGAAGTATTGTTTACTTTCTTAATCATACTCTAAAGTGGGGACTAGAATTTGTGACTACTTACAATTATAAAAATAATATAGCAAGAATTATCAAATAAGAGATAGAGAGGTTGTTTATATACTTGATTATGTTTTCTAATTAGAATTGATGTTCTCTTCA
AAAACTCATGAATTTCCTCGGAGCCATGGAAAGCATACTTCATCGAGATAATAATGTGATATTTAATTATGC

Intron 3 GTCTTTTTCT ... GCAATACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	8	8	0
A2	100	0	4	2
A3	33.33	8	3	5
A4	100	0	3	3
A5	50	1	1	1
A6	27.27	16	7	5
A7	32.43	25	10	14
A8	48.84	11	11	10
A9	11.97	33.1	3	6
A10	16.01	23	6.8	2

>TRINITY_DN101739_c0_g1_i4 from sample A3 / Example spliced isoform

TGGGAATATGAATTTGAATCATTTGGCACTCGTTTACTTCCGCATTATTCCTTCTCAAAATTTCATACATCTACTGTTATTATTTGCTTTTTCATGAAGAAAAACTTACCGCCGAGCATGAAGATGATGAAGAGCAGCAGGAGGTTTTGGGTTTGGCTTCCGCGATTATCATTGTCTCGTTATCAATCCAACAACGTTTT
TTCTTCTTGTTCTTTCCATCCAGAGGCGCCACCACCTCCCGTTCGAGTGGGTTTCACAGAAAACGCGGGCAGAGGAGTTTTCGCTACTCGGGAGATTCGAACCGGCGACCTGATACACACCGCTGATCCCTTTGTGGCTCACCCTTCCCTGGAAGCTTTGGGTCTGCTATTCATGTTTGGGGAGATTGAAAGAAGTAAAA
TGCCATTTGCCAACTTTTTCTTCAAGTTCACAATGGGAGAATGCAAGAGCATCTGCAGATGCTTTACGTGATGGTCAAAACGACATTTCTTTTTGCAGTAGTGAATGCACAGAGCAGGCAAAGGGCTTTTACGAAATCGAGATGAAAATGGATTGGTCGTTATATCATGAGCATTGCAGAGTGAAAGGCTTGAAGTACCC
ATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACCAGCAAATTTTTCTCATGGAATGTCTTCAGCGTGGGAAGATGAGTTTGAGTTATTGGGACACAATTTCAAGAACGCTGACATTCGTCCTGACCTGTTGACTTTTCTTACAAAAGAATGGTACTCTG
GTATTCTGGCTCGCATCCGTGCAAATGTTTTCAGGATTGAATTGATTGGAGGATACCATACCGATCTCCTTGCAGCTGCATCGGCATCTATTTCTGTTGAAGCAACTGTGGGTAGTGCTGTTTACATGCTGCCCTCAATGTACAATCACGATTGTGATCCAAATGCACACATATTGTGGATAGAAAATGCAAAAGCCAGG
TTGAAGGCTCTTTGTAATATTGAATCAGGTGAAGAACTACGTATTTGCTACATCGATGCTAGTATGGATTATGAAGCTCGACAATCTCTACTTTATCAAGGCTTTGGCTTTCGGTGTCAATGCCTTCGATGCAAATCAAAGGACTAGATCTTATATAAAGGATGACGAACTTTTATTAAATGGATTCCATCATAGTGAGA
TATATTCAGAGCTAAAGTTACCCCTTGCATGTTACGAATCATATGACATCTCAAAAGCTTACATACGCAACATTGAAGTATTGTTTACTTTCTTAATCATACTCTAAAGTGGAG

>TRINITY_DN101739_c0_g1_i2 from sample A3 / Example unspliced isoform:

TGGGAATATGAATTTGAATCATTTGGCACTCGTTTACTTCCGCATTATTCCTTCTCAAAATTTCATACATCTACTGTTATTATTTGCTTTTTCATGAAGAAAAACTTACCGCCGAGCATGAAGATGATGAAGAGCAGCAGGAGGTTTTGGGTTTGGCTTCCGCGATTATCATTGTCTCGTTATCAATCCAACAACGTTTT
TTCTTCTTGTTCTTTCCATCCAGgtctttttctgtaatgcccttcaacgcgtcagattcctttacatttcaaggtattttaatgaactaaaagtattagtagcaatattgtgcaataccagAGGCGCCACCACCTCCCGTTCGAGTGGGTTTCACAGAAAACGCGGGCAGAGGAGTTTTCGCTACTCGGGAGATTCGAAC
CGGCGACCTGATACACACCGCTGATCCCTTTGTGGCTCACCCTTCCCTGGAAGCTTTGGGTCTGCTATTCATGTTTGGGGAGATTGAAAGAAGTAAAATGCCATTTGCCAACTTTTTCTTCAAGTTCACAATGGGAGAATGCAAGAGCATCTGCAGATGCTTTACGTGATGGTCAAAACGACATTTCTTTTTGCAGTAGT
GAATGCACAGAGCAGGCAAAGGGCTTTTACGAAATCGAGATGAAAATGGATTGGTCGTTATATCATGAGCATTGCAGAGTGAAAGGCTTGAAGTACCCATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACCAGCAAATTTTTCTCATGGAATGTCTTC
AGCGTGGGAAGATGAGTTTGAGTTATTGGGACACAATTTCAAGAACGCTGACATTCGTCCTGACCTGTTGACTTTTCTTACAAAAGAATGGTACTCTGGTATTCTGGCTCGCATCCGTGCAAATGTTTTCAGGATTGAATTGATTGGAGGATACCATACCGATCTCCTTGCAGCTGCATCGGCATCTATTTCTGTTGAAG
CAACTGTGGGTAGTGCTGTTTACATGCTGCCCTCAATGTACAATCACGATTGTGATCCAAATGCACACATATTGTGGATAGAAAATGCAAAAGCCAGGTTGAAGGCTCTTTGTAATATTGAATCAGGTGAAGAACTACGTATTTGCTACATCGATGCTAGTATGGATTATGAAGCTCGACAATCTCTACTTTATCAAGGC
TTTGGCTTTCGGTGTCAATGCCTTCGATGCAAATCAAAGGACTAGATCTTATATAAAGGATGACGAACTTTTATTAAATGGATTCCATCATAGTGAGATATATTCAGAGCTAAAGTTACCCCTTGCATGTTACGAATCATATGACATCTCAAAAGCTTACATACGCAACATTGAAGTATTGTTTACTTTCTTAATCATAC
TCTAAAGTGGAG

Intron 4 GTCTGCTATT ... GCAGGCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	2	3
A2	100	0	2	8
A3	100	0	3	8
A4	55.56	2	1	4
A5	100	0	2	0
A6	76.19	5	8	24
A7	75.76	8	25	25
A8	80	4	14	18
A9	76.81	8	32	21
A10	87.23	3	26	15

>TRINITY_DN39981_c0_g1_i3 from sample A6 / Example spliced isoform

GTGCACATAAAATGTTTGGTCCTGGGAATATGAATTTGAATCATTTGGCACTCGTTTACTTCCGCATTATTCCTTCTCAAAATTTCATACATCTACTGTTATTATTTGCTTTTTCATGAAGAAAAACTGACCGCCGAGCATGAAGATGATGAAGAGCAGCAGGAGGTTTTGGGTTTGGCTTCCGCGATTATCATTGTCTC
GTTATCAATCCAACAACGTTTTTTCTTCTTGTTCTTTCCATCCAGAGGCGCCACCACCTCCCGTTCGAGTGGGTTTCACAGAAAACGCGGGCAGAGGAGTTTTCGCTACTCGGGAGATTCGAACCGGCGACCTGATACACACCGCTGATCCCTTTGTGGCTCACCCTTCCCTGGAAGCTTTGGGGCTTTTACGAAATCGA
GATGAAAATGGATTGGTCGTTATATCATGAGCATTGCAGAGTGAAAGGCTTGAAGTACCCATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACCAGCAAATTTTTCTCATGGAATGTCTTCAGCGTGGGAAGATGAGTTTGAGTTATTGGGACACAATT
TCAAGAACGCTGACATTCGTCCTGACCTGTTGACTTTTCTTACAAAAGAATGGTACTCTGGTATTCTGGCTCGCATCCGTGCAAATGTTTTCAGGATTGAATTGATTGGAGGATACCATACCGATCTCCTTGCAGCTGCATCGGCATCTATTTCTGTTGAAGCAACTGTGGGTAGTGCTGTTTACATGCTGCCCTCAATG
TACAATCACGATTGTGATCCAAATGCACACATATTGTGGATAGAAAATGCAAAAGCCAGGTTGAAGGCTCTTCGTAATATTGAATCAGGTGAAGAACTACGTATTTGCTACATCGATGCTAGTATGGATTATGAAGCTCGACAATCTCTACTTTATCAAGGCTTTGGCTTTCGGTGTCAATGCCTTCGATGCAAATCAAA
GGACTAGATCTTATATAAAGGATGACGAACTTTTATTAAATGGATTCCATCATAGTGAGATATATTCAGAGCTAAAGTTACCCCTTGCATGTTACGAATCATATGACATCTCAAAAGCTTACATACGCAACATTGAAGTATTGTTTACTTTCTTAATCATACTCTAAAGTGGGGACTAGAATTTGTGACTACTTACAATT
ATAAAAATAATATAGCAAGAATTATCAAATAAGAGATAGAGAGGTTGTTTATATACTTGATTATGTTTTCTAATTAGAATTGATGTTCTCTTCAAAAACTCATGAATTTCCTCGGAGCCATGGAAAGCATACTTCATCGAGATAATAATGTGATATTTAATTATGC

>TRINITY_DN39981_c0_g1_i7 from sample A6 / Example unspliced isoform:

GTGCACATAAAATGTTTGGTCCTGGGAATATGAATTTGAATCATTTGGCACTCGTTTACTTCCGCATTATTCCTTCTCAAAATTTCATACATCTACTGTTATTATTTGCTTTTTCATGAAGAAAAACTGACCGCCGAGCATGAAGATGATGAAGAGCAGCAGGAGGTTTTGGGTTTGGCTTCCGCGATTATCATTGTCTC
GTTATCAATCCAACAACGTTTTTTCTTCTTGTTCTTTCCATCCAGAGGCGCCACCACCTCCCGTTCGAGTGGGTTTCACAGAAAACGCGGGCAGAGGAGTTTTCGCTACTCGGGAGATTCGAACCGGCGACCTGATACACACCGCTGATCCCTTTGTGGCTCACCCTTCCCTGGAAGCTTTGGgtctgctattcatgttt
ggggagattgaaagaagtaaaatgccatttgccaactttttcttcaagttcacaatgggagaatgcaagagcatctgcagatgctttacgtgatggtcaaaacgacatttctttttgcagtagtgaatgcacagagcaggcaaagGGCTTTTACGAAATCGAGATGAAAATGGATTGGTCGTTATATCATGAGCATTGCA
GAGTGAAAGGCTTGAAGTACCCATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACCAGCAAATTTTTCTCATGGAATGTCTTCAGCGTGGGAAGATGAGTTTGAGTTATTGGGACACAATTTCAAGAACGCTGACATTCGTCCTGACCTGTTGACTTTT
CTTACAAAAGAATGGTACTCTGGTATTCTGGCTCGCATCCGTGCAAATGTTTTCAGGATTGAATTGATTGGAGGATACCATACCGATCTCCTTGCAGCTGCATCGGCATCTATTTCTGTTGAAGCAACTGTGGGTAGTGCTGTTTACATGCTGCCCTCAATGTACAATCACGATTGTGATCCAAATGCACACATATTGTG
GATAGAAAATGCAAAAGCCAGGTTGAAGGCTCTTCGTAATATTGAATCAGGTGAAGAACTACGTATTTGCTACATCGATGCTAGTATGGATTATGAAGCTCGACAATCTCTACTTTATCAAGGCTTTGGCTTTCGGTGTCAATGCCTTCGATGCAAATCAAAGGACTAGATCTTATATAAAGGATGACGAACTTTTATTA
AATGGATTCCATCATAGTGAGATATATTCAGAGCTAAAGTTACCCCTTGCATGTTACGAATCATATGACATCTCAAAAGCTTACATACGCAACATTGAAGTATTGTTTACTTTCTTAATCATACTCTAAAGTGGGGACTAGAATTTGTGACTACTTACAATTATAAAAATAATATAGCAAGAATTATCAAATAAGAGATA
GAGAGGTTGTTTATATACTTGATTATGTTTTCTAATTAGAATTGATGTTCTCTTCAAAAACTCATGAATTTCCTCGGAGCCATGGAAAGCATACTTCATCGAGATAATAATGTGATATTTAATTATGC

Intron 5 GTAAGGTTTC ... AAGTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.33	5.2	2	4
A2	49.72	3.2	3	0
A3	47.69	6.6	6	0
A4	50	4	4	1
A5	50	1	1	1
A6	38.3	17.8	11	0
A7	17.46	10	2	2
A8	23.85	9.6	3	1
A9	33.33	24	12	3
A10	17.62	18.8	4	1

>TRINITY_DN39195_c0_g1_i3 from sample A8 / Example spliced isoform

GGGTAGTTCTATGCCAAGAGATGCTTGGTTTGAAATCCACCTTGGTGAGGTGCACTAGTTTATGCTAACAAGTTTACTAGGGATGACATCATGAATGTGGGGAATCATCCCTTTGGTTTCTTGCAAGCTTTTCCTTGGGAATAAAAAGAGAAACGATTTACTTTTTTTTGCTAATTTGTAACTTATAAATTTATATCGTG
TTGTAGGGCTTTTACGAAATCGAGATGAAAATGGATTGGTCGTTATATCATGAGCATTGCAGAGTGAAAGGCTTGAAGTACCCATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACCAGCAAATTTTTCTCATGGAATGTCTTCAGCGTGGGAAGATGA
GTTTGAGTTATTGGGACACAATTTCAAGAACGCTGACATTCGTCCTGACCTGTTGACTTGTATCCTTCAAAATATTTTGAATTATCATGCCAAAGTGCTAGTCTGTATGAGGGGATGTATATCTGAGTGGACTATCTCCAAGGAACTTTTTTCTTCAGCGCTTATGTGGCTTTGTTGTGCTTTGACATGGTGTCAGTTCT
TACAAAAGAATGGTACTCTGGTATTCTGGCTCGCATCCGTGCAAATGTTTTCAGGATTGAATTGATTGGAGGATACCATACCGATCTCCTTGCAGCTGCATCGGCATCTATTTCTGTTGAAGCGACTGTGGGTAGTGCTGTTTACATGCTGCCCTCAATGTACAATCACGATTGTGGTATGTTTCAGCTTCTTCTTGTTT
CTCTTCATTTTTCCTTCTTAATTGAAAGATAACCTGTTGACTATTTGGTGCACTGTAACTCATACACTGTCACTAGCAGCCCTAGCCTTTGGTGGTAATGATAAGGCCAATGTGCCATTTCCAATTGATTTGCAATAGACCATTATGTTGTTTCTGAAATAGACCTATATTTACCTCCTTTCTCAATACTACAGGCAATT
ATGTACTTTATTTCCTGCAATTCAAGGCAAAAAATATAAGCTTATGTCCAA

>TRINITY_DN39195_c0_g1_i5 from sample A8 / Example unspliced isoform:

GGGTAGTTCTATGCCAAGAGATGCTTGGTTTGAAATCCACCTTGGTGAGGTGCACTAGTTTATGCTAACAAGTTTACTAGGGATGACATCATGAATGTGGGGAATCATCCCTTTGGTTTCTTGCAAGCTTTTCCTTGGGAATAAAAAGAGAAACGATTTACTTTTTTTTGCTAATTTGTAACTTATAAATTTATATCGTG
TTGTAGGGCTTTTACGAAATCGAGATGAAAATGGATTGGTCGTTATATCATGAGCATTGCAGAGTGAAAGGCTTGAAGTACCCATTGCTGGTAAAGCGCTTTGCGTGCATGGTTATATCAGGCATGCAATCTGCAGAGAGCCTTGATATTCTTCAACCAGCAAATTTTTCTCATGGAATGTCTTCAGCGgtaaggtttct
cagattctctctagttttatttttatgacaaagcagatgtcatctagtaattgatgggttttcattccaagtattggtgaggtgttcaattactgtgttccgtaactttcctgtgacaatttgtacttccttttatgtcaaatgcgggagtagctgggagtggggtaccagtttggcaactcagttaaacttcgtttctt
ctgcgaagttataatgttacattgtttgaaaatatgcttgaaggatgagtttgtttcttgcaataaatgaaacgcttcaaatttggatgaagatgtctaatacaaactcattatgtgtttcctcaagtttacagTGGGAAGATGAGTTTGAGTTATTGGGACACAATTTCAAGAACGCTGACATTCGTCCTGACCTGTTG
ACTT