sp|Q2HU68|H2A1_MEDTR ----> Probable histone H2A.1

Intron 1 GTATATATTC ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.58	68	8	21
A2	16.38	97	14	24
A3	12.15	94	11	15
A4	17.98	73	15	17
A5	23.61	55	12	22
A6	16.77	675	105	167
A7	17.17	533	116	105
A8	15.42	554	83	119
A9	17.87	586	102	153
A10	14.63	528	80	101

>TRINITY_DN110571_c0_g1_i6 from sample A2 / Example spliced isoform

ATGTCACTGAGCGTATTAAGTCGTTGGGATTTTGTTATTTTTTGAGCAGGCTTTTATTTGGAGTTGAACCAATTTCAGAGGCAGTGGCGATGGATTCTTCAAAGGCTGGTACTGGTAAAGGTGCAGGTGGAAGAAAGGGAGGAGCAAAGAAGAAATCAGTCTCTAGATCCACGAAAGCTGGTCTTCAATTCCCAGTTGCA
AGAATCGGAAGGTATTTGAAGAAGGGTAGATATGCCAAGCGAGTCGGCACAGGAGCTCCTGTGTATCTTGCAGCGGTCATGGAGTATCTTGCAGCTGAGGTTCTTGAATTGGCTGGAAATGCGGCAAAGGATAACAAGAAGAACAGAATTATCCCAAGGCACATTCTTTTGGCTGTAAGGAATGATGATGAGCTTGGGAA
GCTGTTGATGGGTGTGACCATTGCCCATGGAGGAGTGCTGCCTAACATTCACCAGGTGCTTTTGCCTAAGAAGACTGCTGAGAAGACTACTACTAAGGAGCCCAAGTCTCCCAAGAAATGAGCTTGCTTGCTTCATTTAATTTTCTCTTTTGGTGTTTATAAACACCACCTTTAGCTGGAATCGTTATCCATTTAGAGCC
AGTGGTGTCTGCCCGTTTCTTCGCTAAATATGGCCGTATAAGGGTGAGCCTGTCTGTTAGGAATATTGTCTGTAGTTTAAGCTAACTTTTATTAGTACTTGCAGATTGCGTCTGTGAAATAGCTTTTGGAGGGTGAAATGTGATTATTGGGATATCAACCTGGTTGATGTCATATATATATAGTCCTTTGCAAAATTTAA
ATTTAAAAAGGCGCGAAGATTTGGACTGGTCTTTATTTTCTTACTCCCTAATCCTCGGTGATTAAAAGTACTCGTAATCAGCAATGAAGCAAATAACAAAATTCAATCGATCCGTGGGAAATATTGCAGTCTAAAAGCTTTATGAGTAGTCAGATTTTTTCAATATGATTTTGAAAAACAAAGTTAGATTTTTTCAAAAT
CTAGGTCACGC

>TRINITY_DN110571_c0_g1_i1 from sample A2 / Example unspliced isoform:

ATGTCACTGAGCGTATTAAGTCGTTGGGATTTTGTTATTTTTTGAGCAGGCTTTTATTTGGAGTTGAACCAATTTCAGAGGCAGTGGCGATGGATTCTTCAAAGGCTGGTACTGGTAAAGGTGCAGGTGGAAGAAAGGGAGGAGCAAAGAAGAAATCAGTCTCTAGATCCACGAAAGCTGGTCTTCAATTCCCAGTTGCA
AGAATCGGAAGGTATTTGAAGAAGGGTAGATATGCCAAGCGAGTCGGCACAGGAGCTCCTGTGTATCTTGCAGCGGTCATGGAGTATCTTGCAGCTGAGgtatatattcactaatcttgtaatattcttcaattttgtggtgtaagatctgtcgttggaagattggaatggcagggtttgtgcactagggttttgatttc
gcggtctcattttaaatttttggcggttatttattttgcagGTTCTTGAATTGGCTGGAAATGCGGCAAAGGATAACAAGAAGAACAGAATTATCCCAAGGCACATTCTTTTGGCTGTAAGGAATGATGATGAGCTTGGGAAGCTGTTGATGGGTGTGACCATTGCCCATGGAGGAGTGCTGCCTAACATTCACCAGGTG
CTTTTGCCTAAGAAGACTGCTGAGAAGACTACTACTAAGGAGCCCAAGTCTCCCAAGAAATGAGCTTGCTTGCTTCATTTAATTTTCTCTTTTGGTGTTTATAAACACCACCTTTAGCTGGAATCGTTATCCATTTAGAGCCAGTGGTGTCTGCCCGTTTCTTCGCTAAATATGGCCGTATAAGGGTGAGCCTGTCTGTT
AGGAATATTGTCTGTAGTTTAAGCTAACTTTTATTAGTACTTGCAGATTGCGTCTGTGAAATAGCTTTTGGAGGGTGAAATGTGATTATTGGGATATCAACCTGGTTGATGTCATATATATATAGTCCTTTGCAAAATTTAAATTTAAAAAGGCGCGAAGATTTGGACTGGTCTTTATTTTCTTACTCCCTAATCCTCGG
TGATTAAAAGTACTCGTAATCAGCAATGAAGCAAATAACAAAATTCAATCGATCCGTGGGAAATATTGCAGTCTAAAAGCTTTATGAGTAGTCAGATTTTTTCAATATGATTTTGAAAAACAAAGTTAGATTTTTTCAAAATCTAGGTCACGC

Intron 2 GTATATATTC ... TTATGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.03	46	11	13
A2	25	63	3	21
A3	22.22	56	5	16
A4	20.37	43	2	11
A5	22	39	5	11
A6	21.29	414	20	112
A7	19.35	275	4	66
A8	17.04	331	12	68
A9	20.54	352	13	91
A10	19.79	312	6	77

>TRINITY_DN116337_c0_g2_i1 from sample A1 / Example spliced isoform

GTTTTCTAAGTTAATTGAGATCTTTTGAGTAGTTGCAGAGTTAAATTAACAGGTAGGAGCAATGGAGTCTTCAAAGGCTACTGGGAAAGGTGCAGGCGGGAGAAAGGGAGGAGCAAAAAAGAAGTCAGTATCCAGATCTACAAAAGCTGGTCTTCAATTCCCCGTTGCAAGAATCGGAAGGTACCTCAAGAAGGGCAGAT
ATTCCAAGAGAGTCGGCACTGGAGCAGCTGTGTATCTTGCAGCAGTCATGGAATATCTTGCAGCCGAGGTTCTTGAATTGGCTGGAAATGCAGCAAAGGACAACAAGAAGAACAGGATTATTCCAAGGCACATTCTTTTGGCTGTAAGGAACGATGACGAGCTTGGAAAGCTGTTGGTAGGTGTGACCATTGCCCATGGA
GGAGTGCTGCCTAACATTCACCAGGTGCTTCTACCTAAGAAGACTGCAGAGAAGACTACTACTACTAAGGAGCCCAAGTCTCCCAAGAAATAAGCTTGGAAACTTCTTCAAGCTTCTTTTTCTTTTTCAATTGGTGTTTATAAACACCACCTTTGTGTGAAATAATTGACCGCTTTTGAGCAAGAAGGGCCTGACATTGT
GACTGTGAATTAGGGTTAACACTGATTCTAGATTCAGGTATTTCCATTGCCATGGAATAACTCTTCTAGGTTTTGGAAGGTTTGGCTGTCATCAACTCTGTAAATATTCCCCTTTTAAACATTGCAAAGTTAAATAACCAGAACGTTTTATTTATGTTCAATTTAGAACAAATTGGGTTGGAAGGA

>TRINITY_DN116337_c0_g2_i2 from sample A1 / Example unspliced isoform:

GTTTTCTAAGTTAATTGAGATCTTTTGAGTAGTTGCAGAGTTAAATTAACAGGTAGGAGCAATGGAGTCTTCAAAGGCTACTGGGAAAGGTGCAGGCGGGAGAAAGGGAGGAGCAAAAAAGAAGTCAGTATCCAGATCTACAAAAGCTGGTCTTCAATTCCCCGTTGCAAGAATCGGAAGGTACCTCAAGAAGGGCAGAT
ATTCCAAGAGAGTCGGCACTGGAGCAGCTGTGTATCTTGCAGCAGTCATGGAATATCTTGCAGCCGAGgtatatattcactggtttctttttaagaattcgccatttggaattttgaagtattgcgagggcttattttaaattttatgtgcagGTTCTTGAATTGGCTGGAAATGCAGCAAAGGACAACAAGAAGAACAG
GATTATTCCAAGGCACATTCTTTTGGCTGTAAGGAACGATGACGAGCTTGGAAAGCTGTTGGTAGGTGTGACCATTGCCCATGGAGGAGTGCTGCCTAACATTCACCAGGTGCTTCTACCTAAGAAGACTGCAGAGAAGACTACTACTACTAAGGAGCCCAAGTCTCCCAAGAAATAAGCTTGGAAACTTCTTCAAGCTT
CTTTTTCTTTTTCAATTGGTGTTTATAAACACCACCTTTGTGTGAAATAATTGACCGCTTTTGAGCAAGAAGGGCCTGACATTGTGACTGTGAATTAGGGTTAACACTGATTCTAGATTCAGGTATTTCCATTGCCATGGAATAACTCTTCTAGGTTTTGGAAGGTTTGGCTGTCATCAACTCTGTAAATATTCCCCTTT
TAAACATTGCAAAGTTAAATAACCAGAACGTTTTATTTATGTTCAATTTAGAACAAATTGGGTTGGAAGGA