sp|Q8W3L1|MFDR_ARATH ----> NADPH:adrenodoxin oxidoreductase, mitochondrial

Intron 1 GTTAGTTATA ... GCATTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.81	8	4	1
A2	39.13	7	8	1
A3	43.75	9	9	5
A4	77.78	1	5	2
A5	36.84	6	7	0
A6	20	10	2	3
A7	17.24	24	0	10
A8	18.18	18	2	6
A9	21.05	30	5	11
A10	13.33	13	4	0

>TRINITY_DN123210_c4_g1_i8 from sample A3 / Example spliced isoform

GTGCATCGCTTGTATGATATTTAGAGTGCTTTACAACTGAGTATTGATTTGAGGGATTTCATGGGTAAAGGAGTATATTTATCTTTGCCACTAGTGGCATGTAATTTTCAATGGTTTGGTTATATAACTGAGATAATAGACAGAAAATGATGACGATGCTAGAGAATTTTAAGGACCCCATGTTTAATACTCTGTCATTT
TACAGGGAAATGTAGCTCTTGATGTTGCTCGAATGCTTCTTCGTCCATTAAATGAGCTGGCTACCACTGATATTGCAGAACATGCTTTATCTGCTCTCCAGAAGAGTTGCATCAGGAAAGTGTATTTAGTGGGAAGGCGTGGACCTGTCCAAGCAGCTTGCACAGCAAAGGAATTGCGGGAAATTCTTGGTATACAAGGA
GTGAATGTGCACATGAAGGAAGCAGATTTAGTCACCTCATCATTGGATGAGAAAGCCAGAAAGATTTTTACCCTCCATTGACCAGAGAAAGGTGGGTGGAGTGCGCTTAGAGAAGACTATTTTGAAAGAAGATGATACTACCAGCTACCAGTATGCTGTGGGAGCTGGAGAGTTCGAACAGATTGAATCCGGGTTGAAAG
CATTTCAGAGGATATGAGAACCGGCTTGTTAGATACATGTCACTCTTCACATCCTGGAAGATTGGGCCTTCTTGAAGCATTACAAACAAAAGATGTTCAGTATGTGTCATTCAAGGACTGGGAAAGGATTGATGCAAAAGAAAAAGAAATGGGATTTGTGAGAGGTAAACCTAGGGAAAAGATTACTTCATGGCAAGAGT
TGTTGAAAGTTGTAAATGAATAGCTGTGCAGCAAGGAAAATTCTCTTGCCATTTTATCTGCATTCTGAAGCAGCCTATAGTGGTACAGTGATATTATTGTCACCATGAATGCTTAGCAATGTTGCAAACATCATCTCGGCCAAAAAATCGGCAGGGCCC

>TRINITY_DN123210_c4_g1_i9 from sample A3 / Example unspliced isoform:

AACTACGGTCTTCATTTCCTTACCCTTTTATATGCTCGCGGGCCTTTTATCCAATTTACAATACAATATTTTGGACTGTCCGATACAAATGTCTCTGGTTTTCGTAAATTCTAAGGCAGTGGACTGCGAATTTATCCACTCAAACCCCATTATGGAAACGAATTTATTCTGAATTCTTCGCAGTTTGTTGTAAATTCTCT
CTTCAGGGTAGTTCATGGATGCAAGTTCTAAAGTCAGGGATTGAAATGGCTGCAAGATGGGCAAGCACGTGTCTGAGAAAATATTTAAAAGGAAGAAACTATTCCTCCTCCTGTATTTCCGCCAAGCGTTTGCATGTCTGCATTGTAGGGAGCGGGCCGGCTGGATTTTACACAGCAGAGAAGATGCTGAAAAGACATGA
GCAGGCAAATGTAGATATCATAGAAAGATTGCCTACACCATTTGGCTTGGTGCGTTCTGGAGTGGCGCCTGACCATCCCGAAACAAAGGTTCGCGGAAACAAACCCTAAGAATTTTCCTTTGAAGGGAAATGTAGCTCTTGATGTTGCTCGAATGCTTCTTCGTCCATTAAATGAGCTGGCTACCACTGATATTGCAGAA
CATGCTTTATCTGCTCTCCAGAAGAGTTGCATCAGGAAAGTGTATTTAGTGGGAAGGCGTGGACCTGTCCAAGCAGCTTGCACAGCAAAGGAATTGCGGGAAATTCTTGGTATACAAGGAGTGAATGTGCACATGAAGGAAGCAGATTTAGTCACCTCATCATTGGATGAGGAAGAAATGAAAAAGAGTAGAAGTAAAAA
ACGTGTATATGAGCTGTTTTGCAAGGCTGCCAAATTGAAGCCTTCCCAAGAATTCTCAGCCCCTGATCGTGACCTTTACTTTGTTTTCTTTAGAAAGCCAGAAAGATTTTTACCCTCCATTGACCAGAGAAAGGTGGGTGGAGTGCGCTTAGAGAAGACTATTTTGAAAGgttagttatatactgtgactaaaattctat
cagtcaactgtttttcagtgcctgtatattgtacttccctatttttctttattatatagggttttcaattctagagcagtgtctttagttctaccacacttccatgtttcttgcactctcgagcctttaaccttatttaacttgttttcttgcaaaatatttttttcaactcaataaacacttgataagatttactacaa
ttaaaagtcaaaagcctagccaagtgaggctggcttggaaagttgtccaatcattttatttcagttttatggtggtgtaatttaataccagtttgattgcatttacagAAGATGATACTACCAGCTACCAGTATGCTGTGGGAGCTGGAGAGTTCGAACAGATTGAATCCGGGTTGAAAGCATTTCAGAGGATATGAGAA
CCGGCTTGTTAGATACATGTCACTCTTCACATCCTGGAAGATTGGGCCTTCTTGAAGCATTACAAACAAAAGATGTTCAGTATGTGTCATTCAAGGACTGGGAAAGGATTGATGCAAAAGAAAAAGAAATGGGATTTGTGAGAGGTAAACCTAGGGAAAAGATTACTTCATGGCAAGAGTTGTTGAAAGTTGTAAATGAA
TAGCTGTGCAGCAAGGAAAATTCTCTTGCCATTTTATCTGCATTCTGAAGCAGCCTATAGTGGTACAGTGATATTATTGTCACCATGAATGCTTAGCAATGTTGCAAACATCATCTCGGCCAAAAAATCGGCAGGGCCC

Intron 2 GTTCGCGGAA ... TCCTTTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.18	5	5	2
A2	40.43	14	11	8
A3	47.83	6	8	3
A4	31.25	11	7	3
A5	68.42	3	8	5
A6	37.93	9	7	4
A7	33.33	13	8	5
A8	44.44	5	4	4
A9	25.93	10	5	2
A10	21.43	11	2	4

>TRINITY_DN119247_c2_g1_i5 from sample A2 / Example spliced isoform

CTTTTATCCAATTTACAATACAATATTTTGGACTGTCCGATACAAATGTCTCTGGTTTTCGTAAATTCTAAGGCAGTGGACTGCGAATTTATCCACTCAAACCCCATTATGGAAACGAATTTATTCTGAATTCTTCGCAGTTTGTTGTAAATTCTCTCTTCAGGGTAGTTCATGGATGCAAGTTCTAAAGTCAGGGATTG
AAATGGCTGCAAGATGGGCAAGCACGTGTCTGAGAAAATATTTAAAAGGAAGAAACTATTCCTCCTCCTGTATTTCCGCCAAGCGTTTGCATGTCTGCATTGTAGGGAGCGGGCCGGCTGGATTTTACACAGCAGAGAAGATGCTGAAAAGACATGAGCAGGCAAATGTAGATATCATAGAAAGATTGCCTACACCATTT
GGCTTGGTGCGTTCTGGAGTGGCGCCTGACCATCCCGAAACAAAGGTTGTAGTTAACCAGTTTTCTCGAGTTGGTGCAAATGATCGGTGCTCCTTTTTTGGAAATGTATCCTTAGGAAAGGATATATCTCTTACGGAAG

>TRINITY_DN119247_c2_g1_i1 from sample A2 / Example unspliced isoform:

CTTTTATCCAATTTACAATACAATATTTTGGACTGTCCGATACAAATGTCTCTGGTTTTCGTAAATTCTAAGGCAGTGGACTGCGAATTTATCCACTCAAACCCCATTATGGAAACGAATTTATTCTGAATTCTTCGCAGTTTGTTGTAAATTCTCTCTTCAGGGTAGTTCATGGATGCAAGTTCTAAAGTCAGGGATTG
AAATGGCTGCAAGATGGGCAAGCACGTGTCTGAGAAAATATTTAAAAGGAAGAAACTATTCCTCCTCCTGTATTTCCGCCAAGCGTTTGCATGTCTGCATTGTAGGGAGCGGGCCGGCTGGATTTTACACAGCAGAGAAGATGCTGAAAAGACATGAGCAGGCAAATGTAGATATCATAGAAAGATTGCCTACACCATTT
GGCTTGGTGCGTTCTGGAGTGGCGCCTGACCATCCCGAAACAAAGgttcgcggaaacaaaccctaagaattttcctttgaagGTTGTAGTTAACCAGTTTTCTCGAGTTGGTGCAAATGATCGGTGCTCCTTTTTTGGAAATGTATCCTTAGGAAAGGATATATCTCTTACGGATCTTCGCAAACTGTACCATGTGGTAG
TACTTGCTTATGGGGCAGAAAGTGATAGAGAACTTGGTATTCCAGGAGAGAATTTAGCTGGAATTCATTCTGCAAGAGAGTTTGTTTGGTGGTACAATGGTCATCCTGATTGCACCAGTTTTCCGGTGGATTTGCAGAGCACTGATACAGCAGTTGTCCTTGGTCAGGGAAATGTAGCTCTTGATGTTGCTCGAATGCTT
CTTCGTCCATTAAATGAGCTGGCTACCACTGATATTGCAGAACATGCTTTATCTGCTCTCCAGAAGAGTTGCATCAGGAAAGTGTATTTAGTGGGAAGGCGTGGACCTGTCCAAGCAGCTTGCACAGCAAAGGAATTGCGGGAAATTCTTGGGAAATGTAGCTCTTGATGTTGCTCGAATGCTTCTTCGTCCATTAAATG
AGCTGGCTACCACTGATATTGCAGAACATGCTTTATCTGCTCTCCAGAAGAGTTGC

Intron 3 GTATTATTGC ... ATTTTGATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.48	16.3	5	8
A2	28.57	5	4	0
A3	49.35	9	7.3	10
A4	8.84	10.3	1	1
A5	65.45	2.7	5.7	4
A6	13.03	11	3.3	0
A7	15.79	8	3	0
A8	13.04	10	3	0
A9	11.11	4	1	0
A10	22.22	7	4	0

>TRINITY_DN128201_c5_g1_i1 from sample A1 / Example spliced isoform

CCAATTTACAATACAATATTTTGGACTGTCCGATACAAATGTCTCTGGTTTTCGTAAATTCTAAGGCAGTGGACTGCGAATTTATCCACTCAAACCCCATTATGGAAACGAATTTATTCTGAATTCTTCGCAGTTTGTTGTAAATTCTCTCTTCAGGGTAGTTCATGGATGCAAGTTCTAAAGTCAGGGATTGAAATGGC
TGCAAGATGGGCAAGCACGTGTCTGAGAAAATATTTAAAAGGAAGAAACTATTCCTCCTCCTGTATTTCCGCCAAGCGTTTGCATGTCTGCATTGTAGGGAGCGGGCCGGCTGGATTTTACACAGCAGAGAAGATGCTGAAAAGACATGAGCAGGCAAATGTAGATATCATAGAAAGATTGCCTACACCATTTGGCTTGG
TGCGTTCTGGAGTGGCGCCTGACCATCCCGAAACAAAGTTAACCAGTTTTCTCGAGTTGGTGCAAATGATCGGTGCTCCTTTTTTGGAAATGTATCCTTAGGAAAGGATATATCTCTTACGGATCTTCGCAAACTGTACCATGTGGTAGTACTTGCTTATGGGGCAGAAAGTGATAGAGAACTTGGTATTCCAGGAGAGA
ATTTAGCTGGAATTCATTCTGCAAGAGAGTTTGTTTGGTGGTACAATGGTCATCCTGATTGCACCAGTTTTCCGGTGGATTTGCAGAGCACTGATACAGCAGTTGTCCTTGGTCAGGGAAATGTAGCTCTTGATGTTGCTCGAATGCTTCTTCGTCCATTAAATGAGCTGGCTACCACTGATATTGCAGAACATGCTTTA
TCTGCTCTCCAGAAGAGTTGCATCAGGAAAGTGTATTTAGTGGGAAGGCGTGGACCTGTCCAAGCAGCTTGCACAGCAAAGGAATTGCGGGAAATTCTTGGTATACAAGGAGTGAATGTGCACATGAAGGAAGCAGATTTAGTCACCTCATCATTGGATGAGAAAGCCAGAAAGATTTTTACCCTCCATTGACCAGAGAA
AGGTGGGTGGAGTGCGCTTAGAGAAGACTATTTTGAAAGAAGATGATACTACCAGCTACCAGTATGCTGTGGGAGCTGGAGAGTTCGAACAGATTGAATCCGGATTGGTTCTAAAAAGTATTGGATACAAGTCATTGTGTGTAGATGAACTCCCTTTTGACCATAACAGAGGTACACAAAGCTGCAGAAATAATTATGTT
CATTCTCCAACTCATTATGATGTTATGTGTTCACTTCTTGATGAAAATGCTTGCTTGAGACGATTTCAGTCTACTCACATTTTTTCCATAATGATAACATGTCTTTGAGAATTTTGGTGGCATCCTTATACATTGTTGAAAACATCTGATTGTTAAACATAAAACGTGCAATTAAGGACGTTTCTCACAAGTAGTTAAAT
GATAATATTTTTTCCATTTCCTTAATAGTTGATACCTTTTTCTAAAAGTTGATTAAGTTTAATGGTCTCTTAAATAGCTGCAGTGGTAGTAATGCTTTAATCATCTTTTAAAAGGTACAAAGATTTAAAAATTGAATTTGATAAGTGTATTTCACAATGTTTAAGTGGAAGGAAAATAAAAGATTTCATCTTTTCATTTT
CTTGTCTCAATTGAACAACTGTTTCTTTTTCTTGCTGGTCATGGATGGCAGTCAATCCTTTAGCCAATAAAATATTCTTTATAAACTAAGTCACCTGGCACAAAGACATGGGACATGGAAATATGAGTATCCGACACGAACACAATGACCCGTGGAATCAGGAAACGAAGAACACATTATATATGTATATATATATATTT
TAAAATTATATATATTAAAAATATAGACATATAGATCCAATGTTTTTTAG

>TRINITY_DN128201_c5_g1_i7 from sample A1 / Example unspliced isoform:

CCAATTTACAATACAATATTTTGGACTGTCCGATACAAATGTCTCTGGTTTTCGTAAATTCTAAGGCAGTGGACTGCGAATTTATCCACTCAAACCCCATTATGGAAACGAATTTATTCTGAATTCTTCGCAGTTTGTTGTAAATTCTCTCTTCAGGGTAGTTCATGGATGCAAGTTCTAAAGTCAGGGATTGAAATGGC
TGCAAGATGGGCAAGCACGTGTCTGAGAAAATATTTAAAAGGAAGAAACTATTCCTCCTCCTGTATTTCCGCCAAGCGTTTGCATGTCTGCATTGTAGGGAGCGGGCCGGCTGGATTTTACACAGCAGAGAAGATGCTGAAAAGACATGAGCAGGCAAATGTAGATATCATAGAAAGATTGCCTACACCATTTGGCTTGG
TGCGTTCTGGAGTGGCGCCTGACCATCCCGAAACAAAGGTTGTAGTTAACCAGTTTTCTCGAGTTGGTGCAAATGATCGGTGCTCCTTTTTTGGAAATGTATCCTTAGGAAAGGATATATCTCTTACGGATCTTCGCAAACTGTACCATGTGgtattattgccattattagctgtccagatttgatatgaaaatatattt
gttttgtctcttactcgcaaacccattattgcccttaagctcattcttttgatctcaaacaagagctctctataaaatattattgtgattttgatagGTAGTACTTGCTTATGGGGCAGAAAGTGATAGAGAACTTGGTATTCCAGGAGAGGTATGTCCTTGTGTAGTAAATCATTTCATACTTCATTTTGGTGTTTGGT
TGCATGGG

Intron 4 GTTGTAGTTA ... CCTTGGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.82	7	5	15
A2	57.89	12	17	16
A3	40.43	14	8	11
A4	47.37	10	12	6
A5	55.56	6	3	12
A6	55	9	13	9
A7	50	15	16	14
A8	52.38	10	9	13
A9	60	7	11	10
A10	72.97	5	15	12

>TRINITY_DN47684_c4_g2_i12 from sample A7 / Example spliced isoform

CGGGCCTTTTATCCAATTTACAATACAATATTTTGGACTGTCCGATACAAATGTCTCTGGTTTTCGTAAATTCTAAGGCAGTGGACTGCGAATTTATCCACTCAAACCCCATTATGGAAACGAATTTATTCTGAATTCTTCGCAGTTTGTTGTAAATTCTCTCTTCAGGGTAGTTCATGGATGCAAGTTCTAAAGTCAGG
GATTGAAATGGCTGCAAGATGGGCAAGCACGTGTCTGAGAAAATATTTAAAAGGAAGAAACTATTCCTCCTCCTGTATTTCCGCCAAGCGTTTGCATGTCTGCATTGTAGGGAGCGGGCCGGCTGGATTTTACACAGCAGAGAAGATGCTGAAAAGACATGAGCAGGCAAATGTAGATATCATAGAAAGATTGCCTACAC
CATTTGGCTTGGTGCGTTCTGGAGTGGCGCCTGACCATCCCGAAACAAAGGGAAATGTAGCTCTTGATGTTGCTCGAATGCTTCTTCGTCCATTAAATGAGCTGGCTACCACTGATATTGCAGAACATGCTTTATCTGCTCTCCAGAAGAGTTGCATCAGGAAAGTGTATTTAGTGGGAAGGCGTGGACCTGTCCAAGCA
GCTTGCACAGCAAAGGAATTGCGGGAAATTCTTGGTATACAAGGAGTGAATGTGCACATGAAGGAAGCAGATTTAGTCACCTCATCATTGGATGAGGAAGAAATGAAAAAGAGTAGAAGTAAAAAACGTGTATATGAGCTGTTTTGCAAGGCTGCCAAATTGAAGCCTTCCCAAGAATTCTCAGCCCCTGATCGTGACCT
TTACTTTGTTTTCTTTAGAAAGCCAGAAAGATTTTTACCCTCCATTGACCAGAGAAAGGTGGGTGGAGTGCGCTTAGAGAAGACTATTTTGAAAGAAGATGATACTACCAGCTACCAGTATGCTGTGGGAGCTGGAGAGTTCGAACAGATTGAATCCGGATTGGTTCTAAAAAGTATTGGATACAAGTCATTGTGTGTAG
ATGAACTCCCTTTTGACCATAACAGAGGTTGAAAGCATTTCAGAGGATATGAGAACCGGCTTGTTAGATACATGTCACTCTTCACATCCTGGAAGATTGGGCCTTCTTGAAGCATTACAAACAAAAGATGTTCAGTATGTGTCATTCAAGGACTGGGAAAGGATTGATGCAAAAGAAAAAGAAATGGGATTTGTGAGAGG
TAAACCTAGGGAAAAGATTACTTCATGGCAAGAGTTGTTGAAAGTTGTAAATGAATAGCTGTGCAGCAAGGAAAATTCTCTTGCCATTTTATCTGCATTCTGAAGCAGCCTATAGTGGTACAGTGATATTATTGTCACCATGAATGCTTAGCAATGTTGCAAACATCATCTCGGCCAAAAAATCGGCAGGGTCCAGAAAA
CATTATTTCGGAATATCTTGGGAACACATCGGAAAACATCGGCAATATACCAGAAAACATTATTTCGGAATATCTTGGGAACACATCGGAAAACATCGGCAATATATCAGAAAACTTAAAAAAATACAAAAAATATTCTTTTTTTTCAATTTTTTGAACATCTAAAGCAACAATAACATCAAATAAACACATTGCCACAT
GAATATGGAAAGAAAAC

>TRINITY_DN47684_c4_g2_i15 from sample A7 / Example unspliced isoform:

CGGGCCTTTTATCCAATTTACAATACAATATTTTGGACTGTCCGATACAAATGTCTCTGGTTTTCGTAAATTCTAAGGCAGTGGACTGCGAATTTATCCACTCAAACCCCATTATGGAAACGAATTTATTCTGAATTCTTCGCAGTTTGTTGTAAATTCTCTCTTCAGGGTAGTTCATGGATGCAAGTTCTAAAGTCAGG
GATTGAAATGGCTGCAAGATGGGCAAGCACGTGTCTGAGAAAATATTTAAAAGGAAGAAACTATTCCTCCTCCTGTATTTCCGCCAAGCGTTTGCATGTCTGCATTGTAGGGAGCGGGCCGGCTGGATTTTACACAGCAGAGAAGATGCTGAAAAGACATGAGCAGGCAAATGTAGATATCATAGAAAGATTGCCTACAC
CATTTGGCTTGGTGCGTTCTGGAGTGGCGCCTGACCATCCCGAAACAAAGgttgtagttaaccagttttctcgagttggtgcaaatgatcggtgctccttttttggaaatgtatccttaggaaaggatatatctcttacggatcttcgcaaactgtaccatgtggtagtacttgcttatggggcagaaagtgatagagaa
cttggtattccaggagagaatttagctggaattcattctgcaagagagtttgtttggtggtacaatggtcatcctgattgcaccagttttccggtggatttgcagagcactgatacagcagttgtccttggtcagGGAAATGTAGCTCTTGATGTTGCTCGAATGCTTCTTCGTCCATTAAATGAGCTGGCTACCACTGA
TATTGCAGAACATGCTTTATCTGCTCTCCAGAAGAGTTGCATCAGGAAAGTGTATTTAGTGGGAAGGCGTGGACCTGTCCAAGCAGCTTGCACAGCAAAGGAATTGCGGGAAATTCTTGGTATACAAGGAGTGAATGTGCACATGAAGGAAGCAGATTTAGTCACCTCATCATTGGATGAGGAAGAAATGAAAAAGAGTA
GAAGTAAAAAACGTGTATATGAGCTGTTTTGCAAGGCTGCCAAATTGAAGCCTTCCCAAGAATTCTCAGCCCCTGATCGTGACCTTTACTTTGTTTTCTTTAGAAAGCCAGAAAGATTTTTACCCTCCATTGACCAGAGAAAGGTGGGTGGAGTGCGCTTAGAGAAGACTATTTTGAAAGAAGATGATACTACCAGCTAC
CAGTATGCTGTGGGAGCTGGAGAGTTCGAACAGATTGAATCCGGATTGGTTCTAAAAAGTATTGGATACAAGTCATTGTGTGTAGATGAACTCCCTTTTGACCATAACAGAGGTTGAAAGCATTTCAGAGGATATGAGAACCGGCTTGTTAGATACATGTCACTCTTCACATCCTGGAAGATTGGGCCTTCTTGAAGCAT
TACAAACAAAAGATGTTCAGTATGTGTCATTCAAGGACTGGGAAAGGATTGATGCAAAAGAAAAAGAAATGGGATTTGTGAGAGGTAAACCTAGGGAAAAGATTACTTCATGGCAAGAGTTGTTGAAAGTTGTAAATGAATAGCTGTGCAGCAAGGAAAATTCTCTTGCCATTTTATCTGCATTCTGAAGCAGCCTATAG
TGGTACAGTGATATTATTGTCACCATGAATGCTTAGCAATGTTGCAAACATCATCTCGGCCAAAAAATCGGCAGGGTCCAGAAAACATTATTTCGGAATATCTTGGGAACACATCGGAAAACATCGGCAATATACCAGAAAACATTATTTCGGAATATCTTGGGAACACATCGGAAAACATCGGCAATATATCAGAAAAC
TTAAAAAAATACAAAAAATATTCTTTTTTTTCAATTTTTTGAACATCTAAAGCAACAATAACATCAAATAAACACATTGCCACATGAATATGGAAAGAAAACTGGTATATAAGATATATAATATGTTTAGTGATACATCACAGATAAAAAATGAATTGTGTCCTGAATCCCGAGTCATCAAAAAATATAACAAACGTATC
TCATGATCATCATCATGACTCATGAGTCATCTACAATGAAATCTTTGCATTTTCATCCAGTACATTGGTTCCAACTTCTGCTGGATGTGGTAATGCCCTTGAATTGTTTATGTGATTTGACATATTTTCATTGCTAAGCATTTTTATACAAATGTATTTTTTTAACAAAAGAATTGTTTTCTTTGCATTGCTACCAAACC
ATTTTGTAATTACTCTCAGATCTCTTTACTGG

Intron 5 GTTCTATCTT ... TTGATAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40	21	15	13
A2	41.94	9	10	3
A3	20	12	5	1
A4	41.18	10	10	4
A5	28.57	15	3	9
A6	24.14	11	3	4
A7	16	21	3	5
A8	26.32	21	11	4
A9	15.49	30	8	3
A10	11.11	12	3	0

>TRINITY_DN96798_c0_g1_i1 from sample A5 / Example spliced isoform

ATCTGTTTCAAAGCAACATAGGTGTTTAGGTTTAAGCATTTTTATGCCAAGAATTTTTATTTTGTGGTCTTGTTTTTTGCTCTCAGATTGGTTCTAAAAAGTATTGGATACAAGTCATTGTGTGTAGATGAACTCCCTTTTGACCATAACAGAGGGAAATGTAGCTCTTGATGTTGCTCGAATGCTTCTTCGTCCATTAA
ATGAGCTGGCTACCACTGATATTGCAGAACATGCTTTATCTGCTCTCCAGAAGAGTTGCATCAGGAAAGTGTATTTAGTGGGAAGGCGTGGACCTGTCCAAGCAGCTTGCACAGCAAAGGAATTGCGGGAAATTCTTGGTATACAAGGAGTGAATGTGCACATGAAGGAAGCAGATTTAGTCACCTCATCATTGGATGAG


GAAGAAATGAAAAAGAGTAGAAGTAAAAAACGTGTATATGAGCTGTTTTGCAAGGCTGCCAAATTGAAGCCTTCCCAAGAATTCTCAGCCCCTGATCGTGACCTTTACTTTGTTTTCTTTAGAAAGCCAGAAAGATTTTTACCCTCCATTGACCAGAGAAAGGTGGGTGGAGTGCGCTTAGAGAAGACTATTTTGAAAGA
AGATGATACTACCAGCTACCAGTATGCTGTGGGAGCTGGAGAGTTCGAACAGATTGAATCCGGATTGGTTCTAAAAAGTATTGGATACAAGTCATTGTGTGTAGATGAACTCCCTTTTGACCATAACAGAGGGAAATGTAGCTCTTGATGTT

>TRINITY_DN96798_c0_g1_i4 from sample A5 / Example unspliced isoform:

GGCATCAGTTACTTCATTTCCCTTATTTCTATTGTTTTATGGTTGAGGGTTCTCTAATCCATCAATGCCTTTTGACTGACTCAGAATTTAGCTGGAATTCATTCTGCAAGAGAGTTTGTTTGGTGGTACAATGGTCATCCTGATTGCACCAGTTTTCCGGTGGATTTGCAGAGCACTGATACAGCAGTTGTCCTTGGTCA
GGGAAATGTAGCTCTTGATGTTGCTCGAATGCTTCTTCGTCCATTAAATGAGCTGGCTACCACTGATATTGCAGAACATGCTTTATCTGCTCTCCAGAAGAGTTGCATCAGGAAAGTGTATTTAGTGGGAAGGCGTGGACCTGTCCAAGCAGCTTGCACAGCAAAGGAATTGCGGGAAATTCTTGGTATACAAGGAGTGA
ATGTGCACATGAAGGAAGCAGATTTAGTCACCTCATCATTGGATGAGgttctatcttcttttctcggtcctatgatttgaaaagtcaaacctacagtgtttctttttttcctttttcatctgttcaaaataatatctccattgattgagctggtaaacatccaataggcaatgttgtaatttatttgtcaattgtttgtt
aacaaatggttggattctaatgttggcatgtttattatgaatactgtttgtgcattcatggtatttacttgtttggatcttgaattgagaatattaatttttgtttctacaaagtgctcttattgtggttgataacagGAAGAAATGAAAAAGAGTAGAAGTAAAAAACGTGTATATGAGCTGTTTTGCAAGGCTGCCAA
ATTGAAGCCTTCCCAAGAATTCTCAGCCCCTGATCGTGACCTTTACTTTGTTTTCTTTAGAAAGCCAGAAAGATTTTTACCCTCCATTGACCAGAGAAAGGTGGGTGGAGTGCGCTTAGAGAAGACTATTTTGAAAGAAGATGATACTACCAGCTACCAGTATGCTGTGGGAGCTGGAGAGTTCGAACAGATTGAATCCG
GATTGGTTCTAAAAAGTATTGGATACAAGTCATTGTGTGTAGATGAACTCCCTTTTGACCATAACAGAGGGAAATGTAGCTCTTGATGTT