sp|Q06396|ARF1_ORYSJ ----> ADP-ribosylation factor 1

Intron 1 GTTTGTACTT ... TCTTAAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.95	103	17.5	38
A2	20.72	88	17.5	23
A3	31.4	83	16	38
A4	19.23	63	6	15
A5	31.11	31	1.7	14
A6	22.39	454	61.8	131
A7	25.12	477	89.2	160
A8	24.36	431.7	67	139
A9	21.9	492	60.8	138
A10	23.04	354	47.5	106

>TRINITY_DN110434_c7_g3_i6 from sample A3 / Example spliced isoform

TGGAGAAAATATAAAGAATATAGATTAGAATAACAAGTGGAATTCTATTTTTATTTTGAAAGTCATTAATTTCTTCCGTATCGTCCAAGACGACAATCGGCAGCGCAGAGCATTACAGGTGGGTGGCACAGAAGCAGATATGGGGCAGGCATTCCGCAAGCTCTTTGATAGTATCTTCGGCAACAAAGAGATGAGAGTTG
TTATGCTTGGTTTGGATGCAGCTGGTAAGACGACTATATTGTACAAGCTACATATTGGAGAAGTTTTATCTACCGTGCCAACCATTGGGTTTAATGTTGAAAAAGTTCAATACAAGAATGTGCAGTTCACAGTTTGGGATGTGGGTGGCCAGGAGAAGCTCAGGCCTCTTTGGAGGCATTACTTCAACAACACTGATGGC
CTGATATATGTTGTTGATTCATTGGACAGGGAGAGGATTGGAAAGGCCAAGGCAGAATTTCAGGCCATCATCAATGACCCTTTCATGAGGAACAGTGCTATTTTAGTATTTGCAAACAAACAAGATATGAAAGGTGCAATGACTCCAATGGAAATATGCGAAGCTCTTGGGCTTTATGATCTACGAAATAGGAGGTGGCA
TATTCAAGGTACCTGTGCTACAAAAGGAGAAGGCTTATATGAAGGACTGGACTGGTTAGCTACAACTCTGAAAGAAATGCAAGCTAGCGGACTATCTACAGCAGTTGGTGGTTCGAGAATTGGATAAAACATGGGATTATTCTGGTATTTGGAATGGCAGGCGTCCATGTTAGTTGTCATGGGTCTATAACTTGGATATT
TGTCTCTTATATATTTTATGTGAATGAGCATCACACGAACTCACACATGCTGAAGGGAAAGCTATATGCATCATGTACCCTTCGCCATTACGGAGTTTCTCTCTAGCTGCACAACTATTCAATTGTATGCTCAACTTGCCCGTCTTCTGAATACTTTATTGTATATTTTAGCTATGTATAAGCTAACCCTTGCATATGCC
AAGGTACATTTGCACAAATACATTCAGATCGCCACGTGACTGATTTTTAGTCCCTTTGGGCTATTGTTTTCTGCTACGCCAGAACATGGAGGTTTTATTTGTGTTAGATGCGACGACCT

>TRINITY_DN110434_c7_g3_i5 from sample A3 / Example unspliced isoform:

TATTTGCAAACAAACAAGATATGgtttgtacttgtgtttaaaattgctttctttacgttccaactgaactttgatttctattatcttttttttgtaagtacccacggggatttgaacttgtgaccttccatttacaacttctcatctcaattactcattaccaattgctttacctcccgtgggcaactttgattttgatt
atcgtttaaacatttgattgtaatttctggtggtcttaaatagAAAGGTGCAATGACTCCAATGGAAATATGCGAAGCTCTTGGGCTTTATGATCTACGAAATAGGAGGTGGCATATTCAAGGTACCTGTGCTACAAAAGGAGAAGGCTTATATGAAGGACTGGACTGGTTAGCTACAACTCTGAAAGAAATGCAAGCTA
GCGGACTATCTACAGCAGTTGGTGGTTCGAGAATTGGATAAAACATGGTACAACTCATTCCTGACCTTTACCCAGTTAGGTCCCTTTCGCCATAATCACTGACATGATAGACCCTTTTTAATTGGCAGGGATTATTCTGGTATTTGGAATGGCAGGCGTCCATGTTAGTTGTCATGGGTCTATAACTTGGATATTTGTCT
CTTATATATTTTATGTGAATGAGCATCACACGAACTCACACATGCTGAAGGGAAAGCTATATGCATCATGTACCCTTCGCCATTACGGAGTTTCTCTCTAGCTGCACAACTATTCAATTGTATGCTCAACTTGCCCGTCTTCTGAATACTTTATTGTATATTTTAGCTATGTATAAGCTAACCCTTGCATATGCCAAGGT
ACATTTGCACAAATACATTCAGATCGCCACGTGACTGATTTTTAGTCCCTTTGGGCTATTGTTTTCTGCTACGCCAGAACATGGAGGTTTTATTTGTGTTAGATGCGACGACCT

Intron 2 GTTCTGGTTG ... TTATGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	1.08	93	1	1
A2	6.44	89.7	6	2
A3	3.14	63.3	2	0
A4	1.96	51.7	1	0
A5	0	25.3	0	0
A6	0.26	405.7	1	1
A7	0	414.3	0	0
A8	0.56	365.3	2	0
A9	0	374.3	0	0
A10	0	341.7	0	0

>TRINITY_DN113938_c3_g1_i4 from sample A2 / Example spliced isoform

GAAAGTTCGAAGGTACTGTGGAGAAAATATAAAGAATATAGATTAGAATAACAAGTGGAATTCTATTTTTATTTTGAAAGTCATTAATTTCTTCCGTATCGTCCAAGACGACAATCGGCAGAGCAGAGCATTACAGGTGGGTGGCACAGAAGCAGATATGGGGCAGGCATTCCGCAAGCTCTTTGATAGTATCTTCGGCA
ACAAAGAGATGAGAGTTGTTATGCTTGGTTTGGATGCAGCTGGTAAGACGACTATATTGTACAAGCTACATATTGGAGAAGTTTTATCTACCGTGCCAACCATTGGGTTTAATGTTGAAAAAGTTCAATACAAGAATGTGCAGTTCACAGTTTGGGATGTGGGTGGCCAGGAGAAGCTCAGGCCTCTTTGGAGGCATTAC
TTCAACAACACTGATGGCCTGATATATGTTGTTGATTCATTGGACAGGGAGAGGATTGGAAAGGCCAAGGCAGAATTTCAGGCCATCATCAATGACCCTTTCATGAGGAACAGTGCTATTTTAGTATTTGCAAACAAACAAGATATGAAAGGTGCAATGACTCCAATGGAAATATGCGAAGCTCTTGGGCTTTATGATCT
ACGAAATAGGAGGTGGCATATTCAAGGTACCTGTGCTACAAAAGGAGAAGGCTTATATGAAGGACTGGACTGGTTAGCTACAACTCTGAAAGAAATGCAAGCTAGCGGACTATCTACAGCAGTTGGTGGTTCGAGAATTGGATAAAACATGGGATTATTCTGGTATTTGGAATGGCAGGCGTCCATGTTAGTTGTCATGG
GTCTATAACTTGGATATTTGTCTCTTATATATTTTATGTGAATGAGCATCACACGAACTCACACATGCTGAAGGGAAAGCTATATGCATCATGTACCCTTCGCCATTACGGAGTTTCTCTCTAGCTGCACAACTATTCAATTGTATGCTCAACTTGCCCGTCTTCTGAATACTTTATTGTATATTTTAGCTATGTATAAG
CTAACCCTTGCATATGCCAAGGTACATTTGCACAAATACATTCAGATCGCCACGTGACTGATTTTTAGTCCCTTTGGGCTATTGTTTTCTGCTACGCCAGAACATGGAGGTTTTATTTGTGTTAGATGCGACGATCTAAATTTTTATTTTCTAATTTTCCATTGTAAAAAGTGTGACTTCTTGGTTTACTTTGTTCTTAT
TCCAAGTTTGTGACATCCATAAGATGTACATAGTCAAATCTTATCAATTGTGTGGTCGATGCTTTGTTTCAAGCCTTTTATAGCATTCAACAAACTCAAAGTTTGTTAAAGTTTGTGTGGATGATACTATATCATAACAGAAATATAAATGGTACACTCTGAAAGTTTGTGTGGATAAATACTAC

>TRINITY_DN113938_c3_g1_i7 from sample A2 / Example unspliced isoform:

CGACTACCGAGCTTTTATGAGAGAATCATCTCACCTTTGCCTCCAGCTTAAACCCAGCATATGCAGTGTGCACAAGCAGGAATTGGGATTTCATTTTATACTGATTAATGTTCAAATGTGCAGATATATGTTGTTGATTCATTGGACAGGGAGAGGATTGGAAAGGCCAAGGCAGAATTTCAGgttctggttgtgtttaa
tcataatgtgatattctttatgcagctatcatatatttcagtagtttcttgtcttttctgaagctgttattgtcattgtgatattatgttaaagctttgcatttttatgcacagGCCATCATCAATGACCCTTT

Intron 3 GTACAACTCA ... TAATTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.92	97	36	25
A2	31.93	97	54	37
A3	32.54	85	37	45
A4	32.28	64	27	34
A5	26.32	49	19	16
A6	27.93	750	237.3	344
A7	30.72	690	260	352
A8	27.24	633	203	271
A9	30.03	650	254	304
A10	27.65	487.1	163.3	209

>TRINITY_DN118626_c0_g1_i2 from sample A1 / Example spliced isoform

TATAGATTAGAATAACAAGTGGAATTCTATTTTTATTTTGAAAGTCATTAATTTCTTCCGTATCGTCCAAGACGACAATCGGCAGCGCAGAGCATTACAGGTGGGTGGCACAGAAGCAGATATGGGGCAGGCATTCCGCAAGCTCTTTGATAGTATCTTCGGCAACAAAGAGATGAGAGTTGTTATGCTTGGTTTGGATG
CAGCTGGTAAGACGACTATATTGTACAAGCTACATATTGGAGAAGTTTTATCTACCGTGCCAACCATTGGGTTTAATGTTGAAAAAGTTCAATACAAGAATGTGCAGTTCACAGTTTGGGATGTGGGTGGCCAGGAGAAGCTCAGGCCTCTTTGGAGGCATTACTTCAACAACACTGATGGCCTGATATATGTTGTTGAT
TCATTGGACAGGGAGAGGATTGGAAAGGCCAAGGCAGAATTTCAGGCCATCATCAATGACCCTTTCATGAGGAACAGTGCTATTTTAGTATTTGCAAACAAACAAGATATGAAAGGTGCAATGACTCCAATGGAAATATGCGAAGCTCTTGGGCTTTATGATCTACGAAATAGGAGGTGGCATATTCAAGGTACCTGTGC
TACAAAAGGAGAAGGCTTATATGAAGGACTGGACTGGTTAGCTACAACTCTGAAAGAAATGCAAGCTAGCGGACTATCTACAGCAGTTGGTGGTTCGAGAATTGGATAAAACATGGGATTATTCTGGTATTTGGAATGGCAGGCGTCCATGTTAGTTGTCATGGGTCTATAACTTGGATATTTGTCTCTTATATATTTTA
TGTGAATGAGCATCACACGAACTCACACATGCTGAAGGGAAAGCTATATGCATCATGTACCCTTCGCCATTACGGAGTTTCTCTCTAGCTGCACAACTATTCAATTGTATGCTCAACTTGCCCGTCTTCTGAATACTTTATTGTATATTTTAGCTATGTATAAGCTAACCCTTGCATATGCCAAGGTACATTTGCACAAA
TACATTCAGATCGCCACGTGACTGATTTTTAGTCCCTTTGGGCTATTGTTTTCTGCTACGCCAGAACATGGAGGTTTTATTTGTGTTAGATGCGACGATCTAAATTTTTATTTTCTAATTTTCCATTGTAAAAAGTGTGACTTCTTGGTTTACTTTGTTCTTATTCCAAGTTTGTGACATCCATAAGATGTACATAGTCA
AATCTTATCAATTGTGTGGTCGATGCTTTGTTTCAAGCCTTTTATAGCATTCAACAAACTCAAAGTTTGTTAAAGTTTGTGTGGATAATACTATATCATAAC

>TRINITY_DN118626_c0_g1_i4 from sample A1 / Example unspliced isoform:

TATAGATTAGAATAACAAGTGGAATTCTATTTTTATTTTGAAAGTCATTAATTTCTTCCGTATCGTCCAAGACGACAATCGGCAGCGCAGAGCATTACAGGTGGGTGGCACAGAAGCAGATATGGGGCAGGCATTCCGCAAGCTCTTTGATAGTATCTTCGGCAACAAAGAGATGAGAGTTGTTATGCTTGGTTTGGATG
CAGCTGGTAAGACGACTATATTGTACAAGCTACATATTGGAGAAGTTTTATCTACCGTGCCAACCATTGGGTTTAATGTTGAAAAAGTTCAATACAAGAATGTGCAGTTCACAGTTTGGGATGTGGGTGGCCAGGAGAAGCTCAGGCCTCTTTGGAGGCATTACTTCAACAACACTGATGGCCTGATATATGTTGTTGAT
TCATTGGACAGGGAGAGGATTGGAAAGGCCAAGGCAGAATTTCAGGCCATCATCAATGACCCTTTCATGAGGAACAGTGCTATTTTAGTATTTGCAAACAAACAAGATATGAAAGGTGCAATGACTCCAATGGAAATATGCGAAGCTCTTGGGCTTTATGATCTACGAAATAGGAGGTGGCATATTCAAGGTACCTGTGC
TACAAAAGGAGAAGGCTTATATGAAGGACTGGACTGGTTAGCTACAACTCTGAAAGAAATGCAAGCTAGCGGACTATCTACAGCAGTTGGTGGTTCGAGAATTGGATAAAACATGgtacaactcattcctgacctttacccagttaggtccctttcgccataatcactgacatgatagaccctttttaattggcagGGAT
TATTCTGGTATTTGGAATGGCAGGCGTCCATGTTAGTTGTCATGGGTCTATAACTTGGATATTTGTCTCTTATATATTTTATGTGAATGAGCATCACACGAACTCACACATGCTGAAGGGAAAGCTATATGCATCATGTACCCTTCGCCATTACGGAGTTTCTCTCTAGCTGCACAACTATTCAATTGTATGCTCAACTT
GCCCGTCTTCTGAATACTTTATTGTATATTTTAGCTATGTATAAGCTAACCCTTGCATATGCCAAGGTACATTTGCACAAATACATTCAGATCGCCACGTGACTGATTTTTAGTCCCTTTGGGCTATTGTTTTCTGCTACGCCAGAACATGGAGGTTTTATTTGTGTTAGATGCGACGATCTAAATTTTTATTTTCTAAT
TTTCCATTGTAAAAAGTGTGACTTCTTGGTTTACTTTGTTCTTATTCCAAGTTTGTGACATCCATAAGATGTACATAGTCAAATCTTATCAATTGTGTGGTCGATGCTTTGTTTCAAGCCTTTTATAGCATTCAACAAACTCAAAGTTTGTTAAAGTTTGTGTGGATAATACTATATCATAAC