sp|Q9SR22|PAE5_ARATH ----> Pectin acetylesterase 5

Intron 1 GTATTTATAT ... AGCATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.35	32	11	53
A2	57.39	49	0	66
A3	64.1	28	5	50
A4	65.33	26	6	49
A5	58.18	23	7	32
A6	40.91	13	3	9
A7	45.1	28	3	23
A8	32.86	47	7	23
A9	44.19	24	4	19
A10	53.49	20	1	23

>TRINITY_DN42225_c1_g1_i6 from sample A9 / Example spliced isoform

GTTTACTACATATTTAGAAAATATGAAAATTTTCCTTATCATTAAATTTTCGTTTATCATTAAAACAATGAAAAAGCCCCATTAATCTTAACTGGGCTTTGGATGAGGAATATTTTCAAATCTTGGAATCCGATCCATCCATCGTAACAGAAATGTCGGTCTCTAACAAATCAAATCATCGTAACAGAACTGTCAGAAAA
ATCAATTTGTAGTGTAGAGATAATTGGCATTGCTTATCAAACAGGGAGTGGCTCTGGGATCTCAAAGTATTGAGACCGTCCAGACTTCCAGGAATCAATTTACAGTGGCTTTGGGATCTCAAGGCATTGAAACTGTCCAAATTCCCATTGCCATTGGCATTCCCTCGCCTCGCCTTGCCTCTCCTCTTCCTCCTCTCACG
CTGCCTGCATGTGTTGCGTATAGTCTCGTGTTTGATCTGGAGCTCCCTGTAGAGGTGGAGACAGGTAGGCAGGCCCTCAATTTTTGCATAAATAGAAAACCACGAGGAATTCAATTGCAACCATGCCAGTGGAATCGACCATGGGGAATTAAGGTGCAACCATACCAGTGGAATCAACAATGTATGGGAATTCAATTGCA
ACCATACCTTTGTATGGGACAAAGTGTAGCAAACTCTGTTTGAGAGGAAAAAGGATATGGCAAGCTGTAATGGAAGATATTTTGGCACATGGGATGAACAAAGCCGAACAGGCTATTCTTACTGGTTGCTCAGCAGGTGGTCTGGAAACGGTTATTCTCAGCGCTGACTATGTGCAGGATTGGACCCAGGACAGAGCAGA
GGACAGCGGATTCCACTTTACAGCTAATCTACAAGCTCCAACATTGATTGTCCTTATGGCCAAAGTTTCTCTTCATCCAAATTCAGAGGACAAAAGGAATAATATGCATAGCCATGGCATATGAAGTATCTACATGTTCATGATAGAAGACAATAAAAGAGTGTAACATTTATATTTATTTATTATGTAATTACTCTCGA
ATATGTCATAATAAAAAAATTATTATTTTGTTTGAAGGGGCTAAAAAGCCCCTTCTCAATGCCCTTAAGAATGAACCATATGGTTCATTCTTAAGGGTTTGTGCACTCTCTTTTCTCCACTTTTTTTTTTCTTTTTTTTTCAATCTTTAAAAGTAGTTTTTTAAAACGTAATAAATTTTAAATTAAAACTATTTAATAAT
ACATCGA

>TRINITY_DN42225_c1_g1_i7 from sample A9 / Example unspliced isoform:

GTTTACTACATATTTAGAAAATATGAAAATTTTCCTTATCATTAAATTTTCGTTTATCATTAAAACAATGAAAAAGCCCCATTAATCTTAACTGGGCTTTGGATGAGGAATATTTTCAAATCTTGGAATCCGATCCATCCATCGTAACAGAAATGTCGGTCTCTAACAAATCAAATCATCGTAACAGAACTGTCAGAAAA
ATCAATTTGTAGTGTAGAGATAATTGGCATTGCTTATCAAACAGGGAGTGGCTCTGGGATCTCAAAGTATTGAGACCGTCCAGACTTCCAGGAATCAATTTACAGTGGCTTTGGGATCTCAAGGCATTGAAACTGTCCAAATTCCCATTGCCATTGGCATTCCCTCGCCTCGCCTTGCCTCTCCTCTTCCTCCTCTCACG
CTGCCTGCATGTGTTGCGTATAGTCTCGTGTTTGATCTGGAGCTCCCTGTAGAGGTGGAGACAGGTAGGCAGGCCCTCAATTTTTGCATAAATAGAAAACCACGAGGAATTCAATTGCAACCATGCCAGTGGAATCGACCATGGGGAATTAAGGTGCAACCATACCAGTGGAATCAACAATGTATGGGAATTCAATTGCA
ACCATACCTTTGTATGGGACAAAGgtatttatatattccttagacccggtatgttgatttgaatacttagctaacacaattgtaatagtctatcaactaagatcttgcaatttgatatcagttgaaactttcgtacctaattatgagtagcatttcagTGTAGCAAACTCTGTTTGAGAGGAAAAAGGATATGGCAAGCT
GTAATGGAAGATATTTTGGCACATGGGATGAACAAAGCCGAACAGGCTATTCTTACTGGTTGCTCAGCAGGTGGTCTGGAAACGGTTATTCTCAGCGCTGACTATGTGCAGGATTGGACCCAGGACAGAGCAGAGGACAGCGGATTCCACTTTACAGCTAATCTACAAGCTCCAACATTGATTGTCCTTATGGCCAAAGT
TTCTCTTCATCCAAATTCAGAGGACAAAAGGAATAATATGCATAGCCATGGCATATGAAGTATCTACATGTTCATGATAGAAGACAATAAAAGAGTGTAACATTTATATTTATTTATTATGTAATTACTCTCGAATATGTCATAATAAAAAAATTATTATTTTGTTTGAAGGGGCTAAAAAGCCCCTTCTCAATGCCCTT
AAGAATGAACCATATGGTTCATTCTTAAGGGTTTGTGCACTCTCTTTTCTCCACTTTTTTTTTTCTTTTTTTTTCAATCTTTAAAAGTAGTTTTTTAAAACGTAATAAATTTTAAATTAAAACTATTTAATAATACATCGA

Intron 2 GTTGTTGCAA ... TTGATTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.88	25	16	7.3
A2	23.01	37.2	16	6
A3	28.84	55.5	25	19.3
A4	28.01	46.2	22.7	12
A5	27.86	33	14	11.2
A6	15.62	58	13	8.3
A7	25.59	76.5	29	23.3
A8	27.26	136.3	55	44.5
A9	26.95	75.2	26	28.3
A10	28.09	51	25.7	13.7

>TRINITY_DN119235_c0_g4_i4 from sample A3 / Example spliced isoform

CCAGAAAACCATGAGGAATTCCATTGCAACAAAGCCAATGGAATCAACCATGGGGAATTCGATTGCAACCATACCAGTGGAATCAACAATGTACGGACAAAGCATTTCAAGGTAGCAAACTCCATTTGAGAGGACAAAGAATACGGCAAGCTGTAATGGAAGATCTTTTGTCACAGGGAATGAACAAAGCCTAAAAGGCT
ATTCTTACGGGCTGCTCAGCAGGTGGTCTGGCCACGGTTATTCTCAGCGCTGACTACGTGCAGGATTGGACCCAGGACAGAGCAGAGGACAGCGGACTCCACTTTACAGCTAATCTACAAGCTCCAACATTGATTGTCCTTATGAGCCAAAGTTTCTCTTCATCCAAATTCAGAGGACAAAAGAAATAATATGCATGGCC
ATGGCATATGAAGTATCTACATGTTCATGATAGA

>TRINITY_DN119235_c0_g4_i3 from sample A3 / Example unspliced isoform:

CCAGAAAACCATGAGGAATTCCATTGCAACAAAGCCAATGGAATCAACCATGGGGAATTCGATTGCAACCATACCAGTGGAATCAACAATGTACGGACAAAGCATTTCAAGGTAGCAAACTCCATTTGAGAGGACAAAGAATACGGCAAGCTGTAATGGAAGATCTTTTGTCACAGGGAATGAACAAAGCCTAAAAGGCT
ATTCTTACGGGCTGCTCAGCAGGTGGTCTGGCCACGGTTATTCTCAGCGCTGACTACGTGCAGGATTGGACCCAGGACAGAGCAGAGGACAGCGGACTCCACTTTACAGgttgttgcaaatgtattgagtggctatgtttcttttgattttagCTAATCTACAAGCTCCAACATTGATTGTCCTTATGAGCCAAAGTTTC
TCTTCATCCAAATTCAGAGGACAAAAGAAATAATATGCATGGCCATGGCATATGAAGTATCTACATGTTCATGATAGA