sp|Q9XIF8|GSTUG_ARATH ----> Glutathione S-transferase U16

Intron 1 GTAATAACTT ... CATGGGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.05	173	131	189
A2	53.94	118	88.3	188
A3	45.63	196	105	224
A4	47.99	136	76	175
A5	47.62	126.7	74.3	156
A6	48.6	1622	1006.7	2060
A7	48.25	1451	836.3	1870
A8	46.72	1468.3	756.7	1819
A9	47.82	1597.3	897	2031
A10	47.54	1469.7	821.7	1842

>TRINITY_DN109814_c0_g7_i3 from sample A3 / Example spliced isoform

GTATGAGATGGCAATTAAACGACGTGGCATGGGAAAAAGAAACAAATACCGGTGCAAATCAAGAATAGGGGCATATCAATATTTCTAACGGCTCTATTAGTGGACGGGTGCCTGCGGAGCTGTAGGTAGGTTTCGTTCCTATTTATTTACATCGAGGGATGAGGCACATTACATTATATTTTCGGAGTAATGGCAATGGA
AGCCTGTGGAGAAGAGCGGCAAGTGAAGCTGATAGGTGCGAGTCTCCACCCCTTTGTGCTGCGGGTTCGCATAGCACTTGCTCTTAAAGGGATCGATTACCACTTCATTGAAGATGCTGTCATTCAAAATCAGAGCCAGCTGCTGCTGCAATCAAACCCTGTTTACAACAAAATTCCAGTGCTCATCCACAATGGAAAAC
CTATTTGTGAAGCGATGAATATTGTGCAATACATAGACGAGGCATGGGACACCAAGGCGCCAATTCTTATTCCCAAAGATCCATATGACCGAGCCATTGCCCGCTTCTGGGCTTCCTTTGTAGACATTAGGGGTGAACATCCACAGAAAGCGGTTGAAGAGTTGGTGGCAAAGTTTCTTTTCTTGGAGGAAACCCTGCAA
ACAAGCGGCTGCTTGTTGGGAAAAACGTATTTCGGAGGAGCTGAGATCGGCGTTGTTGACATCGTATTGGGTGGTACATCAGTATTCGTCAAAGCCCTCGAGAAGCTTACTAATTCAGTTTTAATAGACGCGGAGAAGATGCCGCTGTTAAGCGCATGGACCGATCGATTCTGTGAGGCCGATGGAGTAAAAGAAATTAT
GCCTGATCCGGTCAAGCTGCTTGAATTTATATGCGCCATGAGAATCAGATTTACATCAACACCTGCTGCATCACCACCTGCTGCATTTGAAGAGATGACAGATAGCACAGAAGACCGTTTATAGATGGATGTTATCCTCTCTCTGTTTTGTAATTTTACTTTTATGTTCTCAGTTTGAATGTTATGATTTCTTTAATAGT
TGGTATTTAATGGCAATGAGTTTCTTGATTATCCATCTTCTTAATTTTATGTTCAGTTTGAAAAAAA

>TRINITY_DN109814_c0_g7_i1 from sample A3 / Example unspliced isoform:

GTATGAGATGGCAATTAAACGACGTGGCATGGGAAAAAGAAACAAATACCGGTGCAAATCAAGAATAGGGGCATATCAATATTTCTAACGGCTCTATTAGTGGACGGGTGCCTGCGGAGCTGTAGGTAGGTTTCGTTCCTATTTATTTACATCGAGGGATGAGGCACATTACATTATATTTTCGGAGTAATGGCAATGGA
AGCCTGTGGAGAAGAGCGGCAAGTGAAGCTGATAGGTGCGAGTCTCCACCCCTTTGTGCTGCGGGTTCGCATAGCACTTGCTCTTAAAGGGATCGATTACCACTTCATTGAAGATGCTGTCATTCAAAATCAGAGCCAGCTGCTGCTGCAATCAAACCCTGTTTACAACAAAATTCCAGTGCTCATCCACAATGGAAAAC
CTATTTGTGAAGCGATGAATATTGTGCAATACATAGACGAGGCATGGGACACCAAGGCGCCAATTCTTATTCCCAAAGATCCATATGACCGAGCCATTGCCCGCTTCTGGGCTTCCTTTGTAGACATTAGGgtaataacttccctctgtaattgcctctcattgctttccttatcgctccctatgtgtttcttgaaaaac
tttctttgtaatccgtatttaatacgctcatgctctttatggctggccacagctcatgccatttcttgggaatgttatcatgggccagGGTGAACATCCACAGAAAGCGGTTGAAGAGTTGGTGGCAAAGTTTCTTTTCTTGGAGGAAACCCTGCAAACAAGCGGCTGCTTGTTGGGAAAAACGTATTTCGGAGGAGCTG
AGATCGGCGTTGTTGACATCGTATTGGGTGGTACATCAGTATTCGTCAAAGCCCTCGAGAAGCTTACTAATTCAGTTTTAATAGACGCGGAGAAGATGCCGCTGTTAAGCGCATGGACCGATCGATTCTGTGAGGCCGATGGAGTAAAAGAAATTATGCCTGATCCGGTCAAGCTGCTTGAATTTATATGCGCCATGAGA
ATCAGATTTACATCAACACCTGCTGCATCACCACCTGCTGCATTTGAAGAGATGACAGATAGCACAGAAGACCGTTTATAGATGGATGTTATCCTCTCTCTGTTTTGTAATTTTACTTTTATGTTCTCAGTTTGAATGTTATGATTTCTTTAATAGTTGGTATTTAATGGCAATGAGTTTCTTGATTATCCATCTTCTTA
ATTTTATGTTCAGTTTGAAAAAAA

Intron 2 GTAATAACTT ... CTGGCCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.87	271	131	111
A2	33.51	213.4	88.1	127
A3	29.57	262	105	115
A4	29.57	187	76	81
A5	30.7	184.1	74.1	89
A6	30.45	2476.1	1003.4	1165
A7	30.3	2148.7	833.3	1035
A8	29.48	1991.6	755.5	910
A9	28.66	2526.6	895.6	1135
A10	31.58	1950.4	820.5	980

>TRINITY_DN115243_c0_g4_i1 from sample A1 / Example spliced isoform

TGGCAATTAAACGACGTGGCATGGGAAAAAGAAACAAATACCGGTGCAAATCAAGAATAGGGGCATATCAATATTTCTAACGGCTCTATTAGTGGACGGGTGCCTGCGGAGCTGTAGGTAGGTTTCGTTCCTATTTATTTACATCGAGGGATGAGGCACATTACATTATATTTTCGGAGTAATGGCAATGGAAGCCTGTG
GAGAAGAGCGGCAAGTGAAGCTGATAGGTGCGAGTCTCCACCCCTTTGTGCTGCGGGTTCGCATAGCACTTGCTCTTAAAGGGATCGATTACCACTTCATTGAAGATGCTGTCATTCAAAATCAGAGCCAGCTGCTGCTGCAATCAAACCCTGTTTACAACAAAATTCCAGTGCTCATCCACAATGGAAAACCTATTTGT
GAAGCGATGAATATTGTGCAATACATAGACGAGGCATGGGACACCAAGGCGCCAATTCTTATTCCCAAAGATCCATATGACCGAGCCATTGCCCGCTTCTGGGCTTCCTTTGTAGACATTAGGCTCATGCCATTTCTTGGGAATGTTATCATGGGCCAGGGTGAACATCCACAGAAAGCGGTTGAAGAGTTGGTGGCAAA
GTTTCTTTTCTTGGAGGAAACCCTGCAAACAAGCGGCTGCTTGTTGGGAAAAACGTATTTCGGAGGAGCTGAGATCGGCGTTGTTGACATCGTATTGGGTGGTACATCAGTATTCGTCAAAGCCCTCGAGAAGCTTACTAATTCAGTTTTAATAGACGCGGAGAAGATGCCGCTGTTAAGCGCATGGACCGATCGATTCT
GTGAGGCCGATGGAGTAAAAGAAATTATGCCTGATCCGGTCAAGCTGCTTGAATTTATATGCGCCATGAGAATCAGATTTACATCAACACCTGCTGCATCACCACCTGCTGCATTTGAAGAGATGACAGATAGCACAGAAGACCGTTTATAGATGGATGTTATCCTCTGTTTTGTAATTTTACTTTTATGTTCTCAGTTT
GAATGTTATCATTTCTTTAATAGTTGGTATTTAATGGCAATGAGTTTCTTGATTAAAAAAA

>TRINITY_DN115243_c0_g4_i2 from sample A1 / Example unspliced isoform:

TGGCAATTAAACGACGTGGCATGGGAAAAAGAAACAAATACCGGTGCAAATCAAGAATAGGGGCATATCAATATTTCTAACGGCTCTATTAGTGGACGGGTGCCTGCGGAGCTGTAGGTAGGTTTCGTTCCTATTTATTTACATCGAGGGATGAGGCACATTACATTATATTTTCGGAGTAATGGCAATGGAAGCCTGTG
GAGAAGAGCGGCAAGTGAAGCTGATAGGTGCGAGTCTCCACCCCTTTGTGCTGCGGGTTCGCATAGCACTTGCTCTTAAAGGGATCGATTACCACTTCATTGAAGATGCTGTCATTCAAAATCAGAGCCAGCTGCTGCTGCAATCAAACCCTGTTTACAACAAAATTCCAGTGCTCATCCACAATGGAAAACCTATTTGT
GAAGCGATGAATATTGTGCAATACATAGACGAGGCATGGGACACCAAGGCGCCAATTCTTATTCCCAAAGATCCATATGACCGAGCCATTGCCCGCTTCTGGGCTTCCTTTGTAGACATTAGGgtaataacttccctctgtaattgcctctcattgctttccttatcgctccctatgtgtttcttgaaaaactttctttg
taatccgtatttaatacgctcatgctctttatggctggccacagCTCATGCCATTTCTTGGGAATGTTATCATGGGCCAGGGTGAACATCCACAGAAAGCGGTTGAAGAGTTGGTGGCAAAGTTTCTTTTCTTGGAGGAAACCCTGCAAACAAGCGGCTGCTTGTTGGGAAAAACGTATTTCGGAGGAGCTGAGATCGGC
GTTGTTGACATCGTATTGGGTGGTACATCAGTATTCGTCAAAGCCCTCGAGAAGCTTACTAATTCAGTTTTAATAGACGCGGAGAAGATGCCGCTGTTAAGCGCATGGACCGATCGATTCTGTGAGGCCGATGGAGTAAAAGAAATTATGCCTGATCCGGTCAAGCTGCTTGAATTTATATGCGCCATGAGAATCAGATT
TACATCAACACCTGCTGCATCACCACCTGCTGCATTTGAAGAGATGACAGATAGCACAGAAGACCGTTTATAGATGGATGTTATCCTCTGTTTTGTAATTTTACTTTTATGTTCTCAGTTTGAATGTTATCATTTCTTTAATAGTTGGTATTTAATGGCAATGAGTTTCTTGATTAAAAAAA